                         SEQUENCE LISTING

<110>  ൺջݿƼ޹˾
 
<120>  ӫøͻ弰Ӧ

<130>  POI213636P

<160>  34    

<170>  PatentIn version 3.3

<210>  1
<211>  540
<212>  DNA
<213>  Artificial

<220>
<223>  źĵҰͽˮӫø(WT Pxluc)Ļ

<400>  1
atgtatataa aagtttggtt tggtctggct tgtctttcat tggttctggc ccaaccaact     60

gaaaacaagc aggagtctca tattgtagat tcagatcttg atggcgaccg tggtaggaag    120

ttgcccggaa aaaagcttcc tatagaagta ctcaaaatca tggaagccaa tgccaggaga    180

gctggttgca ctagaggatg tctcatatgt ctttcaaaaa tcaagtgtac agccaaaatg    240

aagcgataca ttccagggag atgtcatact tatgaaggag ataaatctat tggacaggga    300

ggcataggtg gccctattgt tgatattcct gaaattattg gattcaagaa catggaaccc    360

atggatcagt tcatcgcaca agttgatctg tgcgccgact gtacaactgg gtgcctgaaa    420

ggccttgcta atgttaggtg caatgacttg ctgaagaaat ggctgcctga cagatgtgct    480

ggttttgccg acaaaattca aaatgaagtg gatagtatca agggcatggc tggggatcgc    540


<210>  2
<211>  180
<212>  PRT
<213>  Artificial

<220>
<223>  źĵҰͽˮӫø(WT Pxluc)İ

<400>  2

Met Tyr Ile Lys Val Trp Phe Gly Leu Ala Cys Leu Ser Leu Val Leu 
1               5                   10                  15      


Ala Gln Pro Thr Glu Asn Lys Gln Glu Ser His Ile Val Asp Ser Asp 
            20                  25                  30          


Leu Asp Gly Asp Arg Gly Arg Lys Leu Pro Gly Lys Lys Leu Pro Ile 
        35                  40                  45              


Glu Val Leu Lys Ile Met Glu Ala Asn Ala Arg Arg Ala Gly Cys Thr 
    50                  55                  60                  


Arg Gly Cys Leu Ile Cys Leu Ser Lys Ile Lys Cys Thr Ala Lys Met 
65                  70                  75                  80  


Lys Arg Tyr Ile Pro Gly Arg Cys His Thr Tyr Glu Gly Asp Lys Ser 
                85                  90                  95      


Ile Gly Gln Gly Gly Ile Gly Gly Pro Ile Val Asp Ile Pro Glu Ile 
            100                 105                 110         


Ile Gly Phe Lys Asn Met Glu Pro Met Asp Gln Phe Ile Ala Gln Val 
        115                 120                 125             


Asp Leu Cys Ala Asp Cys Thr Thr Gly Cys Leu Lys Gly Leu Ala Asn 
    130                 135                 140                 


Val Arg Cys Asn Asp Leu Leu Lys Lys Trp Leu Pro Asp Arg Cys Ala 
145                 150                 155                 160 


Gly Phe Ala Asp Lys Ile Gln Asn Glu Val Asp Ser Ile Lys Gly Met 
                165                 170                 175     


Ala Gly Asp Arg 
            180 


<210>  3
<211>  489
<212>  DNA
<213>  Artificial

<220>
<223>  źĵҰͽˮӫøWT-NS PxlucĻ

<400>  3
caaccaactg aaaacaagca ggagtctcat attgtagatt cagatcttga tggcgaccgt     60

ggtaggaagt tgcccggaaa aaagcttcct atagaagtac tcaaaatcat ggaagccaat    120

gccaggagag ctggttgcac tagaggatgt ctcatatgtc tttcaaaaat caagtgtaca    180

gccaaaatga agcgatacat tccagggaga tgtcatactt atgaaggaga taaatctatt    240

ggacagggag gcataggtgg ccctattgtt gatattcctg aaattattgg attcaagaac    300

atggaaccca tggatcagtt catcgcacaa gttgatctgt gcgccgactg tacaactggg    360

tgcctgaaag gccttgctaa tgttaggtgc aatgacttgc tgaagaaatg gctgcctgac    420

agatgtgctg gttttgccga caaaattcaa aatgaagtgg atagtatcaa gggcatggct    480

ggggatcgc                                                            489


<210>  4
<211>  163
<212>  PRT
<213>  Artificial

<220>
<223>  źĵҰͽˮӫøWT-NS Pxlucİ

<400>  4

Gln Pro Thr Glu Asn Lys Gln Glu Ser His Ile Val Asp Ser Asp Leu 
1               5                   10                  15      


Asp Gly Asp Arg Gly Arg Lys Leu Pro Gly Lys Lys Leu Pro Ile Glu 
            20                  25                  30          


Val Leu Lys Ile Met Glu Ala Asn Ala Arg Arg Ala Gly Cys Thr Arg 
        35                  40                  45              


Gly Cys Leu Ile Cys Leu Ser Lys Ile Lys Cys Thr Ala Lys Met Lys 
    50                  55                  60                  


Arg Tyr Ile Pro Gly Arg Cys His Thr Tyr Glu Gly Asp Lys Ser Ile 
65                  70                  75                  80  


Gly Gln Gly Gly Ile Gly Gly Pro Ile Val Asp Ile Pro Glu Ile Ile 
                85                  90                  95      


Gly Phe Lys Asn Met Glu Pro Met Asp Gln Phe Ile Ala Gln Val Asp 
            100                 105                 110         


Leu Cys Ala Asp Cys Thr Thr Gly Cys Leu Lys Gly Leu Ala Asn Val 
        115                 120                 125             


Arg Cys Asn Asp Leu Leu Lys Lys Trp Leu Pro Asp Arg Cys Ala Gly 
    130                 135                 140                 


Phe Ala Asp Lys Ile Gln Asn Glu Val Asp Ser Ile Lys Gly Met Ala 
145                 150                 155                 160 


Gly Asp Arg 
            


<210>  5
<211>  627
<212>  DNA
<213>  Artificial

<220>
<223>  pET28aĺźĵĽˮӫøpET28a Pxluc 
       WT

<400>  5
aagcttgccg ccaccatgta tataaaagtt tggtttggtc tggcttgtct ttcattggtt     60

ctggcccaac caactgaaaa caagcaggag tctcatattg tagattcaga tcttgatggc    120

gaccgtggta ggaagttgcc cggaaaaaag cttcctatag aagtactcaa aatcatggaa    180

gccaatgcca ggagagctgg ttgcactaga ggatgtctca tatgtctttc aaaaatcaag    240

tgtacagcca aaatgaagcg atacattcca gggagatgtc atacttatga aggagataaa    300

tctattggac agggaggcat aggtggccct attgttgata ttcctgaaat tattggattc    360

aagaacatgg aacccatgga tcagttcatc gcacaagttg atctgtgcgc cgactgtaca    420

actgggtgcc tgaaaggcct tgctaatgtt aggtgcaatg acttgctgaa gaaatggctg    480

cctgacagat gtgctggttt tgccgacaaa attcaaaatg aagtggatag tatcaagggc    540

atggctgggg atcgcggtgg cagtgaaaat ctgtattttc agagcggcgg ccatcatcat    600

catcatcatg gcagctgatg agaattc                                        627


<210>  6
<211>  586
<212>  DNA
<213>  Artificial

<220>
<223>  pColdĺźĵĽˮӫø׵Ļ

<400>  6
caccatgaat cacaaagtgc atatgatgta tataaaagtt tggtttggtc tggcttgtct     60

ttcattggtt ctggcccaac caactgaaaa caagcaggag tctcatattg tagattcaga    120

tcttgatggc gaccgtggta ggaagttgcc cggaaaaaag cttcctatag aagtactcaa    180

aatcatggaa gccaatgcca ggagagctgg ttgcactaga ggatgtctca tatgtctttc    240

aaaaatcaag tgtacagcca aaatgaagcg atacattcca gggagatgtc atacttatga    300

aggagataaa tctattggac agggaggcat aggtggccct attgttgata ttcctgaaat    360

tattggattc aagaacatgg aacccatgga tcagttcatc gcacaagttg atctgtgcgc    420

cgactgtaca actgggtgcc tgaaaggcct tgctaatgtt aggtgcaatg acttgctgaa    480

gaaatggctg cctgacagat gtgctggttt tgccgacaaa attcaaaatg aagtggatag    540

tatcaagggc atggctgggg atcgcggtgg cagtgagaac ctgtac                   586


<210>  7
<211>  531
<212>  DNA
<213>  Artificial

<220>
<223>  pColdȥźĵĽˮӫø׵Ļ

<400>  7
catgaatcac aaagtgcata tgcaaccaac tgaaaacaag caggagtctc atattgtaga     60

ttcagatctt gatggcgacc gtggtaggaa gttgcccgga aaaaagcttc ctatagaagt    120

actcaaaatc atggaagcca atgccaggag agctggttgc actagaggat gtctcatatg    180

tctttcaaaa atcaagtgta cagccaaaat gaagcgatac attccaggga gatgtcatac    240

ttatgaagga gataaatcta ttggacaggg aggcataggt ggccctattg ttgatattcc    300

tgaaattatt ggattcaaga acatggaacc catggatcag ttcatcgcac aagttgatct    360

gtgcgccgac tgtacaactg ggtgcctgaa aggccttgct aatgttaggt gcaatgactt    420

gctgaagaaa tggctgcctg acagatgtgc tggttttgcc gacaaaattc aaaatgaagt    480

ggatagtatc aagggcatgg ctggggatcg cggtggcagt gagaacctgt a             531


<210>  8
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer insert-F1WT Pxluc

<400>  8
caccatgaat cacaaagtgc atatgtatat aaaagtttgg                           40


<210>  9
<211>  44
<212>  DNA
<213>  Artificial

<220>
<223>  Primer insert-F2 (WT-NS Pxluc)

<400>  9
catgaatcac aaagtgcata tgcaaccaac tgaaaacaag cagg                      44


<210>  10
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer insert-R

<400>  10
gtacaggttc tcactgccac cgcgatcccc agccatgccc                           40


<210>  11
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer vector-F1

<400>  11
gggcatggct ggggatcgcg gtggcagtga gaacctgtac                           40


<210>  12
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer vector-R1 (WT Pxluc)

<400>  12
ccaaactttt atatacatat gcactttgtg attcatggtg                           40


<210>  13
<211>  44
<212>  DNA
<213>  Artificial

<220>
<223>  Primer vector-R2 (WT-NS Pxluc)

<400>  13
cctgcttgtt ttcagttggt tgcatatgca ctttgtgatt catg                      44


<210>  14
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer F1


<220>
<221>  misc_feature
<222>  (13)..(14)
<223>  n a, c, g,  t

<400>  14
gataaatcta ttnnkcaggg aggcataggt ggccctattg                           40


<210>  15
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer R1


<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n a, c, g, t

<400>  15
tagggccacc tatgcctccc tgmnnaatag atttatctcc                           40


<210>  16
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer F2


<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n a, c, g,  t

<400>  16
gataaatcta ttggannkgg aggcataggt ggccctattg                           40


<210>  17
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer R2


<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  n a, c, g,  t

<400>  17
tagggccacc tatgcctccm nntccaatag atttatctcc                           40


<210>  18
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer F3


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n a, c, g,  t

<400>  18
gataaatcta ttggacagnn kggcataggt ggccctattg                           40


<210>  19
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer R3


<220>
<221>  misc_feature
<222>  (18)..(19)
<223>  n a, c, g, t

<400>  19
tagggccacc tatgccmnnc tgtccaatag atttatctcc                           40


<210>  20
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer F4


<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  n a, c, g,  t

<400>  20
gataaatcta ttggacaggg annkataggt ggccctattg                           40


<210>  21
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer R4


<220>
<221>  misc_feature
<222>  (15)..(16)
<223>  n a, c, g, t

<400>  21
tagggccacc tatmnntccc tgtccaatag atttatctcc                           40


<210>  22
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer F5


<220>
<221>  misc_feature
<222>  (13)..(14)
<223>  n a, c, g,  t

<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n a, c, g,  t

<400>  22
gataaatcta ttnnknnkgg aggcataggt ggccctattg                           40


<210>  23
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer R5


<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  n a, c, g, t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  na, c, g, t

<400>  23
tagggccacc tatgcctccm nnmnnaatag atttatctcc                           40


<210>  24
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer F6


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n  a, c, g, t

<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  n  a, c, g,  t

<400>  24
gataaatcta ttggacagnn knnkataggt ggccctattg                           40


<210>  25
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer R6


<220>
<221>  misc_feature
<222>  (15)..(16)
<223>  n a, c, g,  t

<220>
<221>  misc_feature
<222>  (18)..(19)
<223>  na, c, g,t

<400>  25
tagggccacc tatmnnmnnc tgtccaatag atttatctcc                           40


<210>  26
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer F7


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n  a, c, g,  t

<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  n a, c, g, t

<220>
<221>  misc_feature
<222>  (25)..(26)
<223>  n a, c, g, t

<220>
<221>  misc_feature
<222>  (28)..(29)
<223>  n a, c, g,  t

<400>  26
gaaggagata aatctattnn knnknnknnk ataggtggcc ctattgttga ta             52


<210>  27
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer R7


<220>
<221>  misc_feature
<222>  (18)..(19)
<223>  n a, c, g,  t

<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  na, c, g, t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n a, c, g, t

<220>
<221>  misc_feature
<222>  (27)..(28)
<223>  n  a, c, g,  t

<400>  27
caatagggcc acctatmnnm nnmnnmnnaa tagatttatc tccttcataa gt             52


<210>  28
<211>  585
<212>  DNA
<213>  Artificial

<220>
<223>  pEE12.4ĺźĵĽˮӫø

<400>  28
atgtatataa aagtttggtt tggtctggct tgtctttcat tggttctggc ccaccaccac     60

caccatcacg gcagccaacc aactgaaaac aagcaggagt ctcatattgt agattcagat    120

cttgatggcg accgtggtag gaagttgccc ggaaaaaagc ttcctataga agtactcaaa    180

atcatggaag ccaatgccag gagagctggt tgcactagag gatgtctcat atgtctttca    240

aaaatcaagt gtacagccaa aatgaagcga tacattccag ggagatgtca tacttatgaa    300

ggagataaat ctattggaca gggaggcata ggtggcccta ttgttgatat tcctgaaatt    360

attggattca agaacatgga acccatggat cagttcatcg cacaagttga tctgtgcgcc    420

gactgtacaa ctgggtgcct gaaaggcctt gctaatgtta ggtgcaatga cttgctgaag    480

aaatggctgc ctgacagatg tgctggtttt gccgacaaaa ttcaaaatga agtggatagt    540

atcaagggca tggctgggga tcgcggctcc ggactgaatg atatc                    585


<210>  29
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Primer insert-F

<400>  29
caccaccatc acggcagcca accaactgaa aacaag                               36


<210>  30
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Primer insert-R

<400>  30
gatatcattc agtccggagc cgcgatcccc agccatg                              37


<210>  31
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  

<400>  31
catggctggg gatcgcggct ccggactgaa tgatatc                              37


<210>  32
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  

<400>  32
cttgttttca gttggttggc tgccgtgatg gtggtg                               36


<210>  33
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  

<400>  33
gataaatcta ttggacaggc gcctataggt ggccctattg                           40


<210>  34
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  

<400>  34
tagggccacc tataggcgcc tgtccaatag atttatctcc                           40


