               SEQUENCE LISTING

<110> Buzzard Pharmaceuticals AB

<120> Methods

<130> BUZBN/P80650PC

<140> PCT/EP2022/056269
<141> 2022-03-10

<160> 45

<170> BiSSAP 1.3.6

<210> 1
<211> 153
<212> PRT
<213> Artificial Sequence


<220> 
<223> P01

<400> 1
Ala Pro Val Arg Ser Leu Ala Phe Arg Ile Trp Asp Val Asn Gln Lys 
1               5                   10                  15      
Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala Gly Tyr Leu Gln Gly 
            20                  25                  30          
Pro Asn Val Asn Leu Glu Glu Lys Ile Asp Val Ser Phe Val Gln Gly 
        35                  40                  45              
Glu Glu Ser Asn Asp Lys Ile Pro Val Ala Leu Gly Ile His Gly Gly 
    50                  55                  60                  
Lys Met Cys Leu Ser Cys Val Lys Ser Gly Asp Glu Thr Arg Leu Gln 
65                  70                  75                  80  
Leu Glu Ala Val Asp Pro Lys Asn Tyr Pro Lys Lys Lys Met Glu Lys 
                85                  90                  95      
Arg Phe Val Phe Asn Lys Ile Glu Ile Asn Asn Lys Leu Ser Phe Glu 
            100                 105                 110         
Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Tyr Met Gln Phe Val Ser Ser 
145                 150             

<210> 2
<211> 153
<212> PRT
<213> Artificial Sequence


<220> 
<223> P02

<400> 2
Ala Pro Val Arg Ser Leu Ala Phe Arg Ile Trp Asp Val Asn Gln Lys 
1               5                   10                  15      
Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala Gly Tyr Leu Gln Gly 
            20                  25                  30          
Pro Asn Val Asn Leu Glu Glu Lys Ile Asp Val Ser Phe Val Gln Gly 
        35                  40                  45              
Glu Glu Ser Asn Asp Lys Ile Pro Val Ala Leu Gly Ile His Gly Gly 
    50                  55                  60                  
Lys Met Cys Leu Ser Cys Val Lys Ser Gly Asp Glu Thr Arg Leu Gln 
65                  70                  75                  80  
Leu Glu Ala Val Asp Pro Lys Asn Tyr Pro Lys Lys Lys Met Glu Lys 
                85                  90                  95      
Arg Phe Val Phe Asn Lys Ile Glu Ile Asn Asn Lys Leu Ser Phe Glu 
            100                 105                 110         
Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Tyr Met Gln Phe Val Ser Ser 
145                 150             

<210> 3
<211> 153
<212> PRT
<213> Artificial Sequence


<220> 
<223> P03

<400> 3
Ala Pro Val Arg Ser Leu Ala Phe Arg Ile Trp Asp Val Asn Gln Lys 
1               5                   10                  15      
Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala Gly Tyr Leu Gln Gly 
            20                  25                  30          
Pro Asn Val Asn Leu Glu Glu Lys Phe Ser Met Ser Phe Val Gln Gly 
        35                  40                  45              
Glu Glu Ser Asn Asp Lys Ile Pro Val Ala Leu Gly Leu Lys Glu Lys 
    50                  55                  60                  
Asn Leu Tyr Leu Ser Cys Val Leu Lys Asp Asp Lys Pro Thr Leu Gln 
65                  70                  75                  80  
Leu Glu Ser Val Asp Pro Lys Asn Tyr Pro Lys Lys Lys Met Glu Lys 
                85                  90                  95      
Arg Phe Val Phe Ile Arg Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu 
            100                 105                 110         
Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Thr Met Gln Phe Val Ser Ser 
145                 150             

<210> 4
<211> 153
<212> PRT
<213> Artificial Sequence


<220> 
<223> P04

<400> 4
Ala Pro Val Arg Ser Leu Ala Phe Arg Ile Trp Asp Val Asn Gln Lys 
1               5                   10                  15      
Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala Gly Tyr Leu Gln Gly 
            20                  25                  30          
Pro Asn Val Asn Leu Glu Glu Lys Phe Ser Met Ser Phe Val Gln Gly 
        35                  40                  45              
Glu Glu Ser Asn Asp Lys Ile Pro Val Ala Leu Gly Leu Lys Glu Lys 
    50                  55                  60                  
Asn Leu Tyr Leu Ser Cys Val Leu Lys Asp Asp Lys Pro Thr Leu Gln 
65                  70                  75                  80  
Leu Glu Ser Val Asp Pro Lys Asn Tyr Pro Lys Lys Lys Met Glu Lys 
                85                  90                  95      
Arg Phe Val Phe Asn Lys Ile Glu Ile Asn Asn Lys Leu Glu Phe Glu 
            100                 105                 110         
Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Thr Met Gln Phe Val Ser Ser 
145                 150             

<210> 5
<211> 153
<212> PRT
<213> Artificial Sequence


<220> 
<223> P05

<400> 5
Ala Pro Val Arg Ser Leu Asn Cys Arg Ile Trp Asp Val Asn Gln Lys 
1               5                   10                  15      
Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala Gly Tyr Leu Gln Gly 
            20                  25                  30          
Pro Asn Val Asn Leu Glu Glu Lys Phe Ser Met Ser Phe Val Gln Gly 
        35                  40                  45              
Glu Glu Ser Asn Asp Lys Ile Pro Val Ala Leu Gly Leu Lys Glu Lys 
    50                  55                  60                  
Asn Leu Tyr Leu Ser Cys Val Leu Lys Asp Asp Lys Pro Thr Leu Gln 
65                  70                  75                  80  
Leu Glu Ser Val Asp Pro Lys Asn Tyr Pro Lys Lys Lys Met Glu Lys 
                85                  90                  95      
Arg Phe Val Phe Asn Lys Ile Glu Ile Asn Asn Lys Leu Glu Phe Glu 
            100                 105                 110         
Ser Ala Gln Phe Pro Asn Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Tyr Met Gln Phe Val Ser Ser 
145                 150             

<210> 6
<211> 151
<212> PRT
<213> Homo sapiens


<220> 
<223> IL-1Ra (human)

<400> 6
Arg Pro Ser Gly Arg Lys Ser Ser Lys Met Gln Ala Phe Arg Ile Trp 
1               5                   10                  15      
Asp Val Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala 
            20                  25                  30          
Gly Tyr Leu Gln Gly Pro Asn Val Asn Leu Glu Glu Lys Ile Asp Val 
        35                  40                  45              
Val Pro Ile Glu Pro His Ala Leu Phe Leu Gly Ile His Gly Gly Lys 
    50                  55                  60                  
Met Cys Leu Ser Cys Val Lys Ser Gly Asp Glu Thr Arg Leu Gln Leu 
65                  70                  75                  80  
Glu Ala Val Asn Ile Thr Asp Leu Ser Glu Asn Arg Lys Gln Asp Lys 
                85                  90                  95      
Arg Phe Ala Phe Ile Arg Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu 
            100                 105                 110         
Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             
Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 
Lys Phe Tyr Phe Gln Glu Asp 
145                 150     

<210> 7
<211> 153
<212> PRT
<213> Homo sapiens


<220> 
<223> IL-1b (beta; human)

<400> 7
Ala Pro Val Arg Ser Leu Asn Cys Thr Leu Arg Asp Ser Gln Gln Lys 
1               5                   10                  15      
Ser Leu Val Met Ser Gly Pro Tyr Glu Leu Lys Ala Leu His Leu Gln 
            20                  25                  30          
Gly Gln Asp Met Glu Gln Gln Val Val Phe Ser Met Ser Phe Val Gln 
        35                  40                  45              
Gly Glu Glu Ser Asn Asp Lys Ile Pro Val Ala Leu Gly Leu Lys Glu 
    50                  55                  60                  
Lys Asn Leu Tyr Leu Ser Cys Val Leu Lys Asp Asp Lys Pro Thr Leu 
65                  70                  75                  80  
Gln Leu Glu Ser Val Asp Pro Lys Asn Tyr Pro Lys Lys Lys Met Glu 
                85                  90                  95      
Lys Arg Phe Val Phe Asn Lys Ile Glu Ile Asn Asn Lys Leu Glu Phe 
            100                 105                 110         
Glu Ser Ala Gln Phe Pro Asn Trp Tyr Ile Ser Thr Ser Gln Ala Glu 
        115                 120                 125             
Asn Met Pro Val Phe Leu Gly Gly Thr Lys Gly Gly Gln Asp Ile Thr 
    130                 135                 140                 
Asp Phe Thr Met Gln Phe Val Ser Ser 
145                 150             

<210> 8
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: PCR primer - Kras exon 1F

<400> 8
tcttagagtt ttacacacaa aggtgag                                        27


<210> 9
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: PCR primer - Kras exon 1R

<400> 9
ttcaaagcgg ctggctgc                                                  18


<210> 10
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: PCR primer - Kras exon 2F

<400> 10
ttgagctgtt tacatcacct tg                                             22


<210> 11
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: PCR primer - Kras exon 2R

<400> 11
gcaaagaatc aataaatgta agctatc                                        27


<210> 12
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: PCR primer - Kras exon 3F

<400> 12
gttgagggca aggtttgaag                                                20


<210> 13
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: PCR primer - Kras exon 3R

<400> 13
acgaacacca tctggagcac                                                20


<210> 14
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: PCR primer - Mycoplasma Spp. F

<400> 14
gggagcaaac aggattagat accct                                          25


<210> 15
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: PCR primer - Mycoplasma Spp. R

<400> 15
tgcaccatct gtcactctgt taacctc                                        27


<210> 16
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: qPCR primer - mVcan F

<400> 16
ccagaacgga aatatcaaga ttgg                                           24


<210> 17
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: qPCR primer - mVcan R

<400> 17
tgaaacacaa caccatccac                                                20


<210> 18
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: qPCR primer - mIl1b F

<400> 18
tttgacagtg atgagaatga cc                                             22


<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: qPCR primer - mIl1b R

<400> 19
aatgagtgat actgcctgcc                                                20


<210> 20
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: qPCR primer - mTnf F

<400> 20
gtccccaaag ggatgagaag t                                              21


<210> 21
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: qPCR primer - mTnf R

<400> 21
tttgctacga cgtgggctac                                                20


<210> 22
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: qPCR primer - mGusb F

<400> 22
ttactttaag acgctgatca cc                                             22


<210> 23
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: qPCR primer - mGusb R

<400> 23
acctccaaat gcccatagtc                                                20


<210> 24
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: qPCR primer - hACTB F

<400> 24
gagcacagag cctcgccttt                                                20


<210> 25
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: qPCR primer - hACTB R

<400> 25
tcatcatcca tggtgagctg g                                              21


<210> 26
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: qPCR primer - hVCAN F

<400> 26
gaatgtcact ctaatccctg tc                                             22


<210> 27
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Assay: qPCR primer - hVCAN R

<400> 27
tgtctcggta tcttgctcac                                                20


<210> 28
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> Vcan (shVcan, sc-41904–V) shRNA target sequence 1

<400> 28
caaccaccat cgaaatgaa                                                 19


<210> 29
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> Vcan (shVcan, sc-41904–V) shRNA target sequence 2

<400> 29
ctcacaggct gtaattaca                                                 19


<210> 30
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> Vcan (shVcan, sc-41904–V) shRNA target sequence 3

<400> 30
gtagacatat cccatacta                                                 19


<210> 31
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> Kras (shKras, sc-43876-V) shRNA target sequence 1

<400> 31
ctacaggaaa caagtagta                                                 19


<210> 32
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> Kras (shKras, sc-43876-V) shRNA target sequence 2

<400> 32
gaacagtaga cacgaaaca                                                 19


<210> 33
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> Kras (shKras, sc-43876-V) shRNA target sequence 3

<400> 33
ccattcagtt tccatgtta                                                 19


<210> 34
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Chuk (shChuk, sc-29366-V) shRNA target sequence 1

<400> 34
ccatggtgtt tgaatgtatt t                                              21


<210> 35
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Chuk (shChuk, sc-29366-V) shRNA target sequence 2

<400> 35
ctctcagtgt gttctagatt t                                              21


<210> 36
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Chuk (shChuk, sc-29366-V) shRNA target sequence 3

<400> 36
gcaagcagaa gattattgat t                                              21


<210> 37
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Ikbkb (shIkbkb, sc-35645-V) shRNA target sequence 1

<400> 37
gatgacatct tgaacttgat t                                              21


<210> 38
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Ikbkb (shIkbkb, sc-35645-V) shRNA target sequence 2

<400> 38
ctgcacattt gaatctgtat t                                              21


<210> 39
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Ikbkb (shIkbkb, sc-35645-V) shRNA target sequence 3

<400> 39
cagctctctt agacagttat t                                              21


<210> 40
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Ikbke (shIkbke, sc-39057-V) shRNA target sequence 1

<400> 40
gagatcatgt acagaatcat t                                              21


<210> 41
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Ikbke (shIkbke, sc-39057-V) shRNA target sequence 2

<400> 41
cagtgttgtt tggacaagat t                                              21


<210> 42
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Ikbke (shIkbke, sc-39057-V) shRNA target sequence 3

<400> 42
ccaacaaact agcattactt t                                              21


<210> 43
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Tbk1 (shTbk1, sc-39059-V) shRNA target sequence 1

<400> 43
gtaggactga gatatgaaat t                                              21


<210> 44
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Tbk1 (shTbk1, sc-39059-V) shRNA target sequence 2

<400> 44
gcatcacaga gatttactat t                                              21


<210> 45
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Tbk1 (shTbk1, sc-39059-V) shRNA target sequence 3

<400> 45
gaagttctag tttgcacaat t                                              21


