                         SEQUENCE LISTING

<110>  Syngenta Crop Protection AG
       Syngenta Group Co., Ltd.
       Gao, Yang
       Liu, Chunxia
       Zhang, Yuguo
       Chen, Yanhui
       Liang, Dawei
       Chen, Xi
 
<120>  METHODS AND COMPOSITIONS FOR MODIFYING FLOWERING TIME GENES IN PLANTS

<130>  82490-WO-REG-ORG-P-1

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  582
<212>  DNA
<213>  Glycine max

<400>  1
atgagtttac aatctcaaag cccatcaaag ttcacgaccc tattcatctc ttccaacatg       60

agcaaccctt cagatgaaag ggagcagtgt caaaagaaga ggaaatccac catatgcgaa      120

gcctctaact ttaggacatc aaggagaaga ttctgcagca acaacaaaaa tgaagaggag      180

atgaacaata agggagtttc aacaacactg aagctttacg atgatccttg gaagatcaag      240

aagacgctaa ccgatagcga tttgggaatc ctaagtagac tcttgctggc tgcagatttg      300

gtgaagaaac aaattttgcc tatgttgggt gcatatcatg caagagctgc agaaactgaa      360

gggaccccag ttagagtttg ggacatggac accaaatcca tgcaccaact cgttctaaag      420

cgatggtctt catccaagag ctatgttctt attggaaagt ggaaccaaga tttcgtcaga      480

agaagagatc tcaggaaagg tgatgagatc ggatttcatt gggatccata taattgcgtt      540

ttcaatttct gtgtccttaa acaagctatg ccagagaatt aa                         582


<210>  2
<211>  193
<212>  PRT
<213>  Glycine max

<400>  2

Met Ser Leu Gln Ser Gln Ser Pro Ser Lys Phe Thr Thr Leu Phe Ile 
1               5                   10                  15      


Ser Ser Asn Met Ser Asn Pro Ser Asp Glu Arg Glu Gln Cys Gln Lys 
            20                  25                  30          


Lys Arg Lys Ser Thr Ile Cys Glu Ala Ser Asn Phe Arg Thr Ser Arg 
        35                  40                  45              


Arg Arg Phe Cys Ser Asn Asn Lys Asn Glu Glu Glu Met Asn Asn Lys 
    50                  55                  60                  


Gly Val Ser Thr Thr Leu Lys Leu Tyr Asp Asp Pro Trp Lys Ile Lys 
65                  70                  75                  80  


Lys Thr Leu Thr Asp Ser Asp Leu Gly Ile Leu Ser Arg Leu Leu Leu 
                85                  90                  95      


Ala Ala Asp Leu Val Lys Lys Gln Ile Leu Pro Met Leu Gly Ala Tyr 
            100                 105                 110         


His Ala Arg Ala Ala Glu Thr Glu Gly Thr Pro Val Arg Val Trp Asp 
        115                 120                 125             


Met Asp Thr Lys Ser Met His Gln Leu Val Leu Lys Arg Trp Ser Ser 
    130                 135                 140                 


Ser Lys Ser Tyr Val Leu Ile Gly Lys Trp Asn Gln Asp Phe Val Arg 
145                 150                 155                 160 


Arg Arg Asp Leu Arg Lys Gly Asp Glu Ile Gly Phe His Trp Asp Pro 
                165                 170                 175     


Tyr Asn Cys Val Phe Asn Phe Cys Val Leu Lys Gln Ala Met Pro Glu 
            180                 185                 190         


Asn 
    


<210>  3
<211>  579
<212>  DNA
<213>  Glycine max

<400>  3
atgagtttac aatcacaaat cccatcaaag tttaaaataa tattcatctc ttccaacatg       60

agcaatcatt cagatgagaa ggagcagtgt caaaagaaga ggaaatccac catatgtgaa      120

gcctccaact ttaggacatc aaggagaaga ttctgcagca acaaaaatga agaggagatg      180

aacaagggag tttcaacaac actgaagctt tatgatgacc cttggaagat caagaagacg      240

ctaaccgata gcgatttggg aatcctaagt agactctcgc tggctacaga tttggtgaag      300

aagcaaattt tgcctatgtt gggtgcagat catgcaagag ctgcagaaac tgaagaaggg      360

accccagtta gagtttggga catggacacc aaatccatgc accagctcgt tctaaagcga      420

tggtcttctt ccaagagcta tgttcttatt gcaaagtgga accaagattt cgtgagaaga      480

agagacctca agaaagggga tgagatcgga tttcattggg atccatataa ttgcgttttc      540

aatttctgtg tccttaaacg agctatgcca gagaattaa                             579


<210>  4
<211>  192
<212>  PRT
<213>  Glycine max

<400>  4

Met Ser Leu Gln Ser Gln Ile Pro Ser Lys Phe Lys Ile Ile Phe Ile 
1               5                   10                  15      


Ser Ser Asn Met Ser Asn His Ser Asp Glu Lys Glu Gln Cys Gln Lys 
            20                  25                  30          


Lys Arg Lys Ser Thr Ile Cys Glu Ala Ser Asn Phe Arg Thr Ser Arg 
        35                  40                  45              


Arg Arg Phe Cys Ser Asn Lys Asn Glu Glu Glu Met Asn Lys Gly Val 
    50                  55                  60                  


Ser Thr Thr Leu Lys Leu Tyr Asp Asp Pro Trp Lys Ile Lys Lys Thr 
65                  70                  75                  80  


Leu Thr Asp Ser Asp Leu Gly Ile Leu Ser Arg Leu Ser Leu Ala Thr 
                85                  90                  95      


Asp Leu Val Lys Lys Gln Ile Leu Pro Met Leu Gly Ala Asp His Ala 
            100                 105                 110         


Arg Ala Ala Glu Thr Glu Glu Gly Thr Pro Val Arg Val Trp Asp Met 
        115                 120                 125             


Asp Thr Lys Ser Met His Gln Leu Val Leu Lys Arg Trp Ser Ser Ser 
    130                 135                 140                 


Lys Ser Tyr Val Leu Ile Ala Lys Trp Asn Gln Asp Phe Val Arg Arg 
145                 150                 155                 160 


Arg Asp Leu Lys Lys Gly Asp Glu Ile Gly Phe His Trp Asp Pro Tyr 
                165                 170                 175     


Asn Cys Val Phe Asn Phe Cys Val Leu Lys Arg Ala Met Pro Glu Asn 
            180                 185                 190         


<210>  5
<211>  574
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1-D8 mutant allele

<400>  5
atgagtttac aatctcaaag cccatcaaag ttcacgaccc tattcatctc ttccaacatg       60

agcaaccctt cagatgaaag ggagcagtgt caaaagaaga ggaaatccac catatgcgaa      120

gcctctaact ttaggacatc aaggaggcag caacaacaaa aatgaagagg agatgaacaa      180

taagggagtt tcaacaacac tgaagcttta cgatgatcct tggaagatca agaagacgct      240

aaccgatagc gatttgggaa tcctaagtag actcttgctg gctgcagatt tggtgaagaa      300

acaaattttg cctatgttgg gtgcatatca tgcaagagct gcagaaactg aagggacccc      360

agttagagtt tgggacatgg acaccaaatc catgcaccaa ctcgttctaa agcgatggtc      420

ttcatccaag agctatgttc ttattggaaa gtggaaccaa gatttcgtca gaagaagaga      480

tctcaggaaa ggtgatgaga tcggatttca ttgggatcca tataattgcg ttttcaattt      540

ctgtgtcctt aaacaagcta tgccagagaa ttaa                                  574


<210>  6
<211>  54
<212>  PRT
<213>  Artificial sequence

<220>
<223>  E1-D8 mutant protein

<400>  6

Met Ser Leu Gln Ser Gln Ser Pro Ser Lys Phe Thr Thr Leu Phe Ile 
1               5                   10                  15      


Ser Ser Asn Met Ser Asn Pro Ser Asp Glu Arg Glu Gln Cys Gln Lys 
            20                  25                  30          


Lys Arg Lys Ser Thr Ile Cys Glu Ala Ser Asn Phe Arg Thr Ser Arg 
        35                  40                  45              


Arg Gln Gln Gln Gln Lys 
    50                  


<210>  7
<211>  569
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1-D13 mutant allele

<400>  7
atgagtttac aatctcaaag cccatcaaag ttcacgaccc tattcatctc ttccaacatg       60

agcaaccctt cagatgaaag ggagcagtgt caaaagaaga ggaaatccac catatgcgaa      120

gcctctaact ttaggacatc aagagcaaca acaaaaatga agaggagatg aacaataagg      180

gagtttcaac aacactgaag ctttacgatg atccttggaa gatcaagaag acgctaaccg      240

atagcgattt gggaatccta agtagactct tgctggctgc agatttggtg aagaaacaaa      300

ttttgcctat gttgggtgca tatcatgcaa gagctgcaga aactgaaggg accccagtta      360

gagtttggga catggacacc aaatccatgc accaactcgt tctaaagcga tggtcttcat      420

ccaagagcta tgttcttatt ggaaagtgga accaagattt cgtcagaaga agagatctca      480

ggaaaggtga tgagatcgga tttcattggg atccatataa ttgcgttttc aatttctgtg      540

tccttaaaca agctatgcca gagaattaa                                        569


<210>  8
<211>  56
<212>  PRT
<213>  Artificial sequence

<220>
<223>  E1-D13 mutant protein

<400>  8

Met Ser Leu Gln Ser Gln Ser Pro Ser Lys Phe Thr Thr Leu Phe Ile 
1               5                   10                  15      


Ser Ser Asn Met Ser Asn Pro Ser Asp Glu Arg Glu Gln Cys Gln Lys 
            20                  25                  30          


Lys Arg Lys Ser Thr Ile Cys Glu Ala Ser Asn Phe Arg Thr Ser Arg 
        35                  40                  45              


Ala Thr Thr Lys Met Lys Arg Arg 
    50                  55      


<210>  9
<211>  557
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1-D25 mutant allele

<400>  9
atgagtttac aatctcaaag cccatcaaag ttcacgaccc tattcatctc ttccaacatg       60

agcaaccctt cagatgaaag ggagcagtgt caaaagaaga ggaaatccac catatgcgaa      120

gcctctaact cagcaacaac aaaaatgaag aggagatgaa caataaggga gtttcaacaa      180

cactgaagct ttacgatgat ccttggaaga tcaagaagac gctaaccgat agcgatttgg      240

gaatcctaag tagactcttg ctggctgcag atttggtgaa gaaacaaatt ttgcctatgt      300

tgggtgcata tcatgcaaga gctgcagaaa ctgaagggac cccagttaga gtttgggaca      360

tggacaccaa atccatgcac caactcgttc taaagcgatg gtcttcatcc aagagctatg      420

ttcttattgg aaagtggaac caagatttcg tcagaagaag agatctcagg aaaggtgatg      480

agatcggatt tcattgggat ccatataatt gcgttttcaa tttctgtgtc cttaaacaag      540

ctatgccaga gaattaa                                                     557


<210>  10
<211>  52
<212>  PRT
<213>  Artificial sequence

<220>
<223>  E1-D25 mutant protein

<400>  10

Met Ser Leu Gln Ser Gln Ser Pro Ser Lys Phe Thr Thr Leu Phe Ile 
1               5                   10                  15      


Ser Ser Asn Met Ser Asn Pro Ser Asp Glu Arg Glu Gln Cys Gln Lys 
            20                  25                  30          


Lys Arg Lys Ser Thr Ile Cys Glu Ala Ser Asn Ser Ala Thr Thr Lys 
        35                  40                  45              


Met Lys Arg Arg 
    50          


<210>  11
<211>  573
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1-D9 mutant allele

<400>  11
atgagtttac aatctcaaag cccatcaaag ttcacgaccc tattcatctc ttccaacatg       60

agcaaccctt cagatgaaag ggagcagtgt caaaagaaga ggaaatccac catatgcgaa      120

gcctctaact ttaggacatc aaggagcagc aacaacaaaa atgaagagga gatgaacaat      180

aagggagttt caacaacact gaagctttac gatgatcctt ggaagatcaa gaagacgcta      240

accgatagcg atttgggaat cctaagtaga ctcttgctgg ctgcagattt ggtgaagaaa      300

caaattttgc ctatgttggg tgcatatcat gcaagagctg cagaaactga agggacccca      360

gttagagttt gggacatgga caccaaatcc atgcaccaac tcgttctaaa gcgatggtct      420

tcatccaaga gctatgttct tattggaaag tggaaccaag atttcgtcag aagaagagat      480

ctcaggaaag gtgatgagat cggatttcat tgggatccat ataattgcgt tttcaatttc      540

tgtgtcctta aacaagctat gccagagaat taa                                   573


<210>  12
<211>  190
<212>  PRT
<213>  Artificial sequence

<220>
<223>  E1-D9 mutant protein

<400>  12

Met Ser Leu Gln Ser Gln Ser Pro Ser Lys Phe Thr Thr Leu Phe Ile 
1               5                   10                  15      


Ser Ser Asn Met Ser Asn Pro Ser Asp Glu Arg Glu Gln Cys Gln Lys 
            20                  25                  30          


Lys Arg Lys Ser Thr Ile Cys Glu Ala Ser Asn Phe Arg Thr Ser Arg 
        35                  40                  45              


Ser Ser Asn Asn Lys Asn Glu Glu Glu Met Asn Asn Lys Gly Val Ser 
    50                  55                  60                  


Thr Thr Leu Lys Leu Tyr Asp Asp Pro Trp Lys Ile Lys Lys Thr Leu 
65                  70                  75                  80  


Thr Asp Ser Asp Leu Gly Ile Leu Ser Arg Leu Leu Leu Ala Ala Asp 
                85                  90                  95      


Leu Val Lys Lys Gln Ile Leu Pro Met Leu Gly Ala Tyr His Ala Arg 
            100                 105                 110         


Ala Ala Glu Thr Glu Gly Thr Pro Val Arg Val Trp Asp Met Asp Thr 
        115                 120                 125             


Lys Ser Met His Gln Leu Val Leu Lys Arg Trp Ser Ser Ser Lys Ser 
    130                 135                 140                 


Tyr Val Leu Ile Gly Lys Trp Asn Gln Asp Phe Val Arg Arg Arg Asp 
145                 150                 155                 160 


Leu Arg Lys Gly Asp Glu Ile Gly Phe His Trp Asp Pro Tyr Asn Cys 
                165                 170                 175     


Val Phe Asn Phe Cys Val Leu Lys Gln Ala Met Pro Glu Asn 
            180                 185                 190 


<210>  13
<211>  582
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1-S15 mutant allele

<400>  13
atgagtttac aatctcaaag cccatcaaag ttcacgaccc tattcatctc ttccaacatg       60

agcaaccctt cagatgaaag ggagcagtgt caaaagaaga ggaaatccac catatgcgaa      120

gcctctaact ttaggacatc aaggagtttc aacaacatga acaacaaaaa tgaagaggag      180

atgaacaata agggagtttc aacaacactg aagctttacg atgatccttg gaagatcaag      240

aagacgctaa ccgatagcga tttgggaatc ctaagtagac tcttgctggc tgcagatttg      300

gtgaagaaac aaattttgcc tatgttgggt gcatatcatg caagagctgc agaaactgaa      360

gggaccccag ttagagtttg ggacatggac accaaatcca tgcaccaact cgttctaaag      420

cgatggtctt catccaagag ctatgttctt attggaaagt ggaaccaaga tttcgtcaga      480

agaagagatc tcaggaaagg tgatgagatc ggatttcatt gggatccata taattgcgtt      540

ttcaatttct gtgtccttaa acaagctatg ccagagaatt aa                         582


<210>  14
<211>  193
<212>  PRT
<213>  Artificial sequence

<220>
<223>  E1-S15 mutant protein

<400>  14

Met Ser Leu Gln Ser Gln Ser Pro Ser Lys Phe Thr Thr Leu Phe Ile 
1               5                   10                  15      


Ser Ser Asn Met Ser Asn Pro Ser Asp Glu Arg Glu Gln Cys Gln Lys 
            20                  25                  30          


Lys Arg Lys Ser Thr Ile Cys Glu Ala Ser Asn Phe Arg Thr Ser Arg 
        35                  40                  45              


Ser Phe Asn Asn Met Asn Asn Lys Asn Glu Glu Glu Met Asn Asn Lys 
    50                  55                  60                  


Gly Val Ser Thr Thr Leu Lys Leu Tyr Asp Asp Pro Trp Lys Ile Lys 
65                  70                  75                  80  


Lys Thr Leu Thr Asp Ser Asp Leu Gly Ile Leu Ser Arg Leu Leu Leu 
                85                  90                  95      


Ala Ala Asp Leu Val Lys Lys Gln Ile Leu Pro Met Leu Gly Ala Tyr 
            100                 105                 110         


His Ala Arg Ala Ala Glu Thr Glu Gly Thr Pro Val Arg Val Trp Asp 
        115                 120                 125             


Met Asp Thr Lys Ser Met His Gln Leu Val Leu Lys Arg Trp Ser Ser 
    130                 135                 140                 


Ser Lys Ser Tyr Val Leu Ile Gly Lys Trp Asn Gln Asp Phe Val Arg 
145                 150                 155                 160 


Arg Arg Asp Leu Arg Lys Gly Asp Glu Ile Gly Phe His Trp Asp Pro 
                165                 170                 175     


Tyr Asn Cys Val Phe Asn Phe Cys Val Leu Lys Gln Ala Met Pro Glu 
            180                 185                 190         


Asn 
    


<210>  15
<211>  572
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1Lb-D7 mutant allele

<400>  15
atgagtttac aatcacaaat cccatcaaag tttaaaataa tattcatctc ttccaacatg       60

agcaatcatt cagatgagaa ggagcagtgt caaaagaaga ggaaatccac catatgtgaa      120

gcctccaact ttaggacatc aaggagaaca gcaacaaaaa tgaagaggag atgaacaagg      180

gagtttcaac aacactgaag ctttatgatg acccttggaa gatcaagaag acgctaaccg      240

atagcgattt gggaatccta agtagactct cgctggctac agatttggtg aagaagcaaa      300

ttttgcctat gttgggtgca gatcatgcaa gagctgcaga aactgaagaa gggaccccag      360

ttagagtttg ggacatggac accaaatcca tgcaccagct cgttctaaag cgatggtctt      420

cttccaagag ctatgttctt attgcaaagt ggaaccaaga tttcgtgaga agaagagacc      480

tcaagaaagg ggatgagatc ggatttcatt gggatccata taattgcgtt ttcaatttct      540

gtgtccttaa acgagctatg ccagagaatt aa                                    572


<210>  16
<211>  57
<212>  PRT
<213>  Artificial sequence

<220>
<223>  E1Lb-D7 mutant protein

<400>  16

Met Ser Leu Gln Ser Gln Ile Pro Ser Lys Phe Lys Ile Ile Phe Ile 
1               5                   10                  15      


Ser Ser Asn Met Ser Asn His Ser Asp Glu Lys Glu Gln Cys Gln Lys 
            20                  25                  30          


Lys Arg Lys Ser Thr Ile Cys Glu Ala Ser Asn Phe Arg Thr Ser Arg 
        35                  40                  45              


Arg Thr Ala Thr Lys Met Lys Arg Arg 
    50                  55          


<210>  17
<211>  440
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1Lb-D139 mutant allele

<400>  17
atgagtttac aatctgcagc aacaaaaatg aagaggagat gaacaaggga gtttcaacaa       60

cactgaagct ttatgatgac ccttggaaga tcaagaagac gctaaccgat agcgatttgg      120

gaatcctaag tagactctcg ctggctacag atttggtgaa gaagcaaatt ttgcctatgt      180

tgggtgcaga tcatgcaaga gctgcagaaa ctgaagaagg gaccccagtt agagtttggg      240

acatggacac caaatccatg caccagctcg ttctaaagcg atggtcttct tccaagagct      300

atgttcttat tgcaaagtgg aaccaagatt tcgtgagaag aagagacctc aagaaagggg      360

atgagatcgg atttcattgg gatccatata attgcgtttt caatttctgt gtccttaaac      420

gagctatgcc agagaattaa                                                  440


<210>  18
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  E1Lb-D139 mutant protein

<400>  18

Met Ser Leu Gln Ser Ala Ala Thr Lys Met Lys Arg Arg 
1               5                   10              


<210>  19
<211>  576
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1Lb-D3 mutant allele

<400>  19
atgagtttac aatcacaaat cccatcaaag tttaaaataa tattcatctc ttccaacatg       60

agcaatcatt cagatgagaa ggagcagtgt caaaagaaga ggaaatccac catatgtgaa      120

gcctccaact ttaggacatc aaggagaatc tgcagcaaca aaaatgaaga ggagatgaac      180

aagggagttt caacaacact gaagctttat gatgaccctt ggaagatcaa gaagacgcta      240

accgatagcg atttgggaat cctaagtaga ctctcgctgg ctacagattt ggtgaagaag      300

caaattttgc ctatgttggg tgcagatcat gcaagagctg cagaaactga agaagggacc      360

ccagttagag tttgggacat ggacaccaaa tccatgcacc agctcgttct aaagcgatgg      420

tcttcttcca agagctatgt tcttattgca aagtggaacc aagatttcgt gagaagaaga      480

gacctcaaga aaggggatga gatcggattt cattgggatc catataattg cgttttcaat      540

ttctgtgtcc ttaaacgagc tatgccagag aattaa                                576


<210>  20
<211>  191
<212>  PRT
<213>  Artificial sequence

<220>
<223>  E1Lb-D3 mutant protein

<400>  20

Met Ser Leu Gln Ser Gln Ile Pro Ser Lys Phe Lys Ile Ile Phe Ile 
1               5                   10                  15      


Ser Ser Asn Met Ser Asn His Ser Asp Glu Lys Glu Gln Cys Gln Lys 
            20                  25                  30          


Lys Arg Lys Ser Thr Ile Cys Glu Ala Ser Asn Phe Arg Thr Ser Arg 
        35                  40                  45              


Arg Ile Cys Ser Asn Lys Asn Glu Glu Glu Met Asn Lys Gly Val Ser 
    50                  55                  60                  


Thr Thr Leu Lys Leu Tyr Asp Asp Pro Trp Lys Ile Lys Lys Thr Leu 
65                  70                  75                  80  


Thr Asp Ser Asp Leu Gly Ile Leu Ser Arg Leu Ser Leu Ala Thr Asp 
                85                  90                  95      


Leu Val Lys Lys Gln Ile Leu Pro Met Leu Gly Ala Asp His Ala Arg 
            100                 105                 110         


Ala Ala Glu Thr Glu Glu Gly Thr Pro Val Arg Val Trp Asp Met Asp 
        115                 120                 125             


Thr Lys Ser Met His Gln Leu Val Leu Lys Arg Trp Ser Ser Ser Lys 
    130                 135                 140                 


Ser Tyr Val Leu Ile Ala Lys Trp Asn Gln Asp Phe Val Arg Arg Arg 
145                 150                 155                 160 


Asp Leu Lys Lys Gly Asp Glu Ile Gly Phe His Trp Asp Pro Tyr Asn 
                165                 170                 175     


Cys Val Phe Asn Phe Cys Val Leu Lys Arg Ala Met Pro Glu Asn 
            180                 185                 190     


<210>  21
<211>  573
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1Lb-D6 mutant allele

<400>  21
atgagtttac aatcacaaat cccatcaaag tttaaaataa tattcatctc ttccaacatg       60

agcaatcatt cagatgagaa ggagcagtgt caaaagaaga ggaaatccac catatgtgaa      120

gcctccaact ttaggacatc aaggagaagc agcaacaaaa atgaagagga gatgaacaag      180

ggagtttcaa caacactgaa gctttatgat gacccttgga agatcaagaa gacgctaacc      240

gatagcgatt tgggaatcct aagtagactc tcgctggcta cagatttggt gaagaagcaa      300

attttgccta tgttgggtgc agatcatgca agagctgcag aaactgaaga agggacccca      360

gttagagttt gggacatgga caccaaatcc atgcaccagc tcgttctaaa gcgatggtct      420

tcttccaaga gctatgttct tattgcaaag tggaaccaag atttcgtgag aagaagagac      480

ctcaagaaag gggatgagat cggatttcat tgggatccat ataattgcgt tttcaatttc      540

tgtgtcctta aacgagctat gccagagaat taa                                   573


<210>  22
<211>  190
<212>  PRT
<213>  Artificial sequence

<220>
<223>  E1Lb-D6 mutant protein

<400>  22

Met Ser Leu Gln Ser Gln Ile Pro Ser Lys Phe Lys Ile Ile Phe Ile 
1               5                   10                  15      


Ser Ser Asn Met Ser Asn His Ser Asp Glu Lys Glu Gln Cys Gln Lys 
            20                  25                  30          


Lys Arg Lys Ser Thr Ile Cys Glu Ala Ser Asn Phe Arg Thr Ser Arg 
        35                  40                  45              


Arg Ser Ser Asn Lys Asn Glu Glu Glu Met Asn Lys Gly Val Ser Thr 
    50                  55                  60                  


Thr Leu Lys Leu Tyr Asp Asp Pro Trp Lys Ile Lys Lys Thr Leu Thr 
65                  70                  75                  80  


Asp Ser Asp Leu Gly Ile Leu Ser Arg Leu Ser Leu Ala Thr Asp Leu 
                85                  90                  95      


Val Lys Lys Gln Ile Leu Pro Met Leu Gly Ala Asp His Ala Arg Ala 
            100                 105                 110         


Ala Glu Thr Glu Glu Gly Thr Pro Val Arg Val Trp Asp Met Asp Thr 
        115                 120                 125             


Lys Ser Met His Gln Leu Val Leu Lys Arg Trp Ser Ser Ser Lys Ser 
    130                 135                 140                 


Tyr Val Leu Ile Ala Lys Trp Asn Gln Asp Phe Val Arg Arg Arg Asp 
145                 150                 155                 160 


Leu Lys Lys Gly Asp Glu Ile Gly Phe His Trp Asp Pro Tyr Asn Cys 
                165                 170                 175     


Val Phe Asn Phe Cys Val Leu Lys Arg Ala Met Pro Glu Asn 
            180                 185                 190 


<210>  23
<211>  570
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1Lb-D9 mutant allele

<400>  23
atgagtttac aatcacaaat cccatcaaag tttaaaataa tattcatctc ttccaacatg       60

agcaatcatt cagatgagaa ggagcagtgt caaaagaaga ggaaatccac catatgtgaa      120

gcctccaact ttaggacatc aaggagaagc aacaaaaatg aagaggagat gaacaaggga      180

gtttcaacaa cactgaagct ttatgatgac ccttggaaga tcaagaagac gctaaccgat      240

agcgatttgg gaatcctaag tagactctcg ctggctacag atttggtgaa gaagcaaatt      300

ttgcctatgt tgggtgcaga tcatgcaaga gctgcagaaa ctgaagaagg gaccccagtt      360

agagtttggg acatggacac caaatccatg caccagctcg ttctaaagcg atggtcttct      420

tccaagagct atgttcttat tgcaaagtgg aaccaagatt tcgtgagaag aagagacctc      480

aagaaagggg atgagatcgg atttcattgg gatccatata attgcgtttt caatttctgt      540

gtccttaaac gagctatgcc agagaattaa                                       570


<210>  24
<211>  189
<212>  PRT
<213>  Artificial sequence

<220>
<223>  E1Lb-D9 mutant protein

<400>  24

Met Ser Leu Gln Ser Gln Ile Pro Ser Lys Phe Lys Ile Ile Phe Ile 
1               5                   10                  15      


Ser Ser Asn Met Ser Asn His Ser Asp Glu Lys Glu Gln Cys Gln Lys 
            20                  25                  30          


Lys Arg Lys Ser Thr Ile Cys Glu Ala Ser Asn Phe Arg Thr Ser Arg 
        35                  40                  45              


Arg Ser Asn Lys Asn Glu Glu Glu Met Asn Lys Gly Val Ser Thr Thr 
    50                  55                  60                  


Leu Lys Leu Tyr Asp Asp Pro Trp Lys Ile Lys Lys Thr Leu Thr Asp 
65                  70                  75                  80  


Ser Asp Leu Gly Ile Leu Ser Arg Leu Ser Leu Ala Thr Asp Leu Val 
                85                  90                  95      


Lys Lys Gln Ile Leu Pro Met Leu Gly Ala Asp His Ala Arg Ala Ala 
            100                 105                 110         


Glu Thr Glu Glu Gly Thr Pro Val Arg Val Trp Asp Met Asp Thr Lys 
        115                 120                 125             


Ser Met His Gln Leu Val Leu Lys Arg Trp Ser Ser Ser Lys Ser Tyr 
    130                 135                 140                 


Val Leu Ile Ala Lys Trp Asn Gln Asp Phe Val Arg Arg Arg Asp Leu 
145                 150                 155                 160 


Lys Lys Gly Asp Glu Ile Gly Phe His Trp Asp Pro Tyr Asn Cys Val 
                165                 170                 175     


Phe Asn Phe Cys Val Leu Lys Arg Ala Met Pro Glu Asn 
            180                 185                 


<210>  25
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1Lb forward primer

<400>  25
gttatcatgc ttgaaaatga tgatg                                             25


<210>  26
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1Lb reverse primer

<400>  26
gtgcatagat ctcatgctag acct                                              24


<210>  27
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1 forward primer

<400>  27
tccattgttt gtagcgcatg gtc                                               23


<210>  28
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1 reverse primer

<400>  28
catagatctc ctgctagcta gacc                                              24


<210>  29
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1 sequencing primer

<400>  29
gctagtagtg taaacactca aaacac                                            26


<210>  30
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  E1Lb sequencing primer

<400>  30
tgcctttcta ctggctatga c                                                 21


<210>  31
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Cas12a forward primer

<400>  31
gggcaagctc tacatgttcc ag                                                22


<210>  32
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Cas12a reverse primer

<400>  32
ccgtggttgt tctcgtcga                                                    19


<210>  33
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Adh Forward primer

<400>  33
aggtgtggat cgggctgtt                                                    19


<210>  34
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Adh Reverse primer

<400>  34
catcgtggac gcattcga                                                     18


<210>  35
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Cas12a Probe

<400>  35
cgtggctctt gtcggagaag tccttgtt                                          28


<210>  36
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Adh Probe

<400>  36
actggcagca tccaagccat ggtct                                             25


<210>  37
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  gRNA (PAM: TTTA)

<400>  37
tttaggacat caaggagaag attctgc                                           27


