<110>	Sahin, Fikret
<120>	AN IMMUNOGENIC COMPOSITION OF DISEASE-ASSOCIATED ANTIGENS FOR USE IN A VACCINE, ANTIBODY PRODUCTION AND IMMUNODIAGNOSTIC TESTS
<130>	3001-52431PCT
<140>	PCT/TR2022/050090
<141>	2022-02-01
<150>	None
<151>	None
<160>	4
<210>	1 
<211>	1273 
<212>	PRT
<213>	Severe acute respiratory syndrome coronavirus 2
<220>
<223> PRT  of surface glycoprotein (Spike protein) complete-wt

<400>	1

   
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys 
                5                   10                  15
Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn 
                20                  25                  30
Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
                35                  40                  45
Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser 
                50                  55                  60
Asn Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly 
                65                  70                  75
Thr Lys Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val 
                80                  85                  90
Tyr Phe Ala Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile 
                95                  100                 105
Phe Gly Thr Thr Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val 
                110                 115                 120
Asn Asn Ala Thr Asn Val Val Ile Lys Val Cys Glu Phe Gln Phe 
                125                 130                 135
Cys Asn Asp Pro Phe Leu Gly Val Tyr Tyr His Lys Asn Asn Lys 
                140                 145                 150
Ser Trp Met Glu Ser Glu Phe Arg Val Tyr Ser Ser Ala Asn Asn 
                155                 160                 165
Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp Leu Glu 
                170                 175                 180
Gly Lys Gln Gly Asn Phe Lys Asn Leu Met Glu Phe Val Phe Lys 
                185                 190                 195
Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile 
                200                 205                 210
Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro 
                215                 220                 225
Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
                230                 235                 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser 
                245                 250                 255
Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu 
                260                  265                270
Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
                275                 280                 285
Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys 
                290                 295                 300
Cys Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr 
                305                 310                 315
Ser Asn Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro 
                320                 325                 330
Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr 
                335                 340                 345
Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn 
                350                 355                 360
Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser 
                365                 370                 375
Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu 
                380                 385                 390
Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp 
                395                 400                 405
Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp 
                410                 415                 420
Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
                425                 430                 435
Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn 
                440                 445                 450
Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu 
                455                 460                 465
Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
                470                 475                 480
Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr 
                485                 490                 495
Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
                500                 505                 510
Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
                515                 520                 525
Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn 
                530                 535                 540
Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser 
                545                 550                 555
Asn Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala 
                560                 565                 570
Asp Thr Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu 
                575                 580                 585
Asp Ile Thr Pro Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro 
                590                 595                 600
Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu Tyr Gln Gly Val 
                605                 610                 615
Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp Gln Leu Thr 
                620                 625                 630
Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe Gln Thr 
                635                 640                 645
Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser Tyr 
                650                 655                 660
Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln 
                665                 670                 675
Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala Ser Gln 
                680                 685                 690
Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val 
                695                 700                 705
Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
                710                 715                 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser 
                725                 730                 735
Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser 
                 740                745                 750
Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
                755                 760                 765
Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu 
                770                 775                 780
Val Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys 
                785                 790                 795
Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser 
                800                 805                 810
Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys 
                815                 820                 825
Val Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys 
                830                 835                 840
Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe 
                845                 850                 855
Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile 
                860                 865                 870
Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly 
                875                 880                 885
Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met 
                890                 895                 900
Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val 
                905                 910                 915
Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala 
                920                 925                 930
Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu 
                935                 940                 945
Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
                950                 955                 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser 
                965                 970                 975
Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu 
                980                 985                 990
Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
                995                 1000                1005
Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala 
                1010                1015                1020
Ser Ala Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly 
                1025                1030                1035
Gln Ser Lys Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met 
                1040                1045                1050
Ser Phe Pro Gln Ser Ala Pro His Gly Val Val Phe Leu His Val 
                1055                1060                1065
Thr Tyr Val Pro Ala Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala 
                1070                1075                1080
Ile Cys His Asp Gly Lys Ala His Phe Pro Arg Glu Gly Val Phe 
                1085                1090                1095
Val Ser Asn Gly Thr His Trp Phe Val Thr Gln Arg Asn Phe Tyr 
                1100                1105                1110
Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr Phe Val Ser Gly Asn 
                1115                1120                1125
Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr Val Tyr Asp Pro 
                1130                1135                1140
Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys Tyr 
                1145                1150                1155
Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile Ser 
                1160                1165                1170
Gly Ile Asn Ala Ser Phe Val Asn Ile Gln Lys Glu Ile Asp Arg 
                1175                1180                1185
Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 
                1190                1195                1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr 
                1205                1210                1215
Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val 
                1220                1225                1230
Thr Ile Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys 
                1235                1240                1245
Gly Cys Cys Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp 
                1250                1255                1260
Ser Glu Pro Val Leu Lys Gly Val Lys Leu His Tyr Thr   
                165                 1270             

<210>	2 
<211>	1252
<212>	PRT
<213>	Severe acute respiratory syndrome coronavirus 2
<220>	

<223>	Incomplete surface glycoprotein (Spike protein Δ21). 21 amino acids removed from the C-terminal region. 

<400>   2

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys 
                5                   10                  15
Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn 
                20                  25                  30
Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser 
                35                  40                  45
Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser 
                50                  55                  60
Asn Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly 
                65                  70                  75
Thr Lys Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val 
                80                  85                  90
Tyr Phe Ala Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile 
                95                  100                 105
Phe Gly Thr Thr Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val 
                110                 115                 120
Asn Asn Ala Thr Asn Val Val Ile Lys Val Cys Glu Phe Gln Phe 
                125                 130                 135
Cys Asn Asp Pro Phe Leu Gly Val Tyr Tyr His Lys Asn Asn Lys 
                140                 145                 150
Ser Trp Met Glu Ser Glu Phe Arg Val Tyr Ser Ser Ala Asn Asn 
                155                 160                 165
Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp Leu Glu 
                170                 175                 180
Gly Lys Gln Gly Asn Phe Lys Asn Leu Met Glu Phe Val Phe Lys 
                185                 190                 195
Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile 
                200                 205                 210
Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro 
                215                 220                 225
Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
                230                 235                 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser 
                245                 250                 255
Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu 
                260                 265                 270
Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile 
                275                 280                 285
Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys 
                290                 295                 300
Cys Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr 
                305                 310                 315
Ser Asn Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro 
                320                 325                 330
Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr 
                335                 340                 345
Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn 
                350                 355                 360
Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser 
                365                 370                 375
Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu 
                380                 385                 390
Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp 
                395                 400                 405
Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp 
                410                 415                 420
Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
                425                 430                 435
Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn 
                440                 445                 450
Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu 
                455                 460                 465
Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
                470                 475                 480
Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr 
                485                 490                 495
Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
                500                 505                 510
Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
                515                 520                 525
Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn 
                530                 535                 540
Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser 
                545                 550                 555
Asn Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala 
                560                 565                 570
Asp Thr Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu 
                575                 580                 585
Asp Ile Thr Pro Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro 
                590                 595                 600
Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu Tyr Gln Gly Val 
                605                 610                 615
Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp Gln Leu Thr 
                620                 625                 630
Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe Gln Thr 
                635                 640                 645
Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser Tyr 
                650                 655                 660
Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln 
                665                 670                 675
Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala Ser Gln 
                680                 685                 690
Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val 
                695                 700                 705
Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
                710                 715                 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser 
                725                 730                 735
Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser 
                 740                745                 750
Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg 
                755                 760                 765
Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu 
                770                 775                 780
Val Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys 
                785                 790                 795
Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser 
                800                 805                 810
Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys 
                815                 820                 825
Val Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys 
                830                 835                 840
Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe 
                845                 850                 855
Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile 
                860                 865                 870
Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly 
                875                 880                 885
Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met 
                890                 895                 900
Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val 
                905                 910                 915
Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala 
                920                 925                 930
Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu 
                935                 940                 945
Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
                950                 955                 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser 
                965                 970                 975
Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu 
                980                 985                 990
Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
                995                 1000                1005
Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala 
                1010                1015                1020
Ser Ala Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly 
                1025                1030                1035
Gln Ser Lys Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met 
                1040                1045                1050
Ser Phe Pro Gln Ser Ala Pro His Gly Val Val Phe Leu His Val 
                1055                1060                1065
Thr Tyr Val Pro Ala Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala 
                1070                1075                1080
Ile Cys His Asp Gly Lys Ala His Phe Pro Arg Glu Gly Val Phe 
                1085                1090                1095
Val Ser Asn Gly Thr His Trp Phe Val Thr Gln Arg Asn Phe Tyr 
                1100                1105                1110
Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr Phe Val Ser Gly Asn 
                1115                1120                1125
Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr Val Tyr Asp Pro 
                1130                1135                1140
Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys Tyr 
                1145                1150                1155
Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile Ser 
                1160                1165                1170
Gly Ile Asn Ala Ser Phe Val Asn Ile Gln Lys Glu Ile Asp Arg 
                1175                1180                1185
Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 
                1190                1195                1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr 
                1205                1210                1215
Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val 
                1220                1225                1230
Thr Ile Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys 
                1235                1240                1245
Gly Cys Cys Ser Cys Gly Ser 
                1250            

<210>	3 
<211>	3822 
<212>	DNA
<213>	Severe acute respiratory syndrome coronavirus 2
<220>	

<223>	wild type cDNA of the surface glycoprotein (Spike protein-wt), codon optimized for mouse

<400>	3



   
atgttcgtct ttctggtgct cctgcctctc gtgtcatcac agtgcgtcaa tctcacaact 60
cggactcagc tcccccctgc ttatacaaac agcttcacaa ggggcgtgta ctaccctgac 120
aaggtgttta gaagctccgt gctgcactcc acacaggatc tgtttctgcc attcttttct 180
aacgtgacct ggttccacgc tatccacgtg tccggcacca acggaacaaa gaggttcgac 240
aaccccgtgc tgccttttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc 300
atcagaggct ggatctttgg aaccacactg gacagcaaga cacagtccct gctgatcgtg 360
aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa cgatcccttc 420
ctgggcgtgt actaccacaa gaacaacaag tcttggatgg agagcgagtt tcgggtgtac 480
tctagcgcca acaactgcac atttgagtac gtgtcccagc ctttcctgat ggacctggag 540
ggcaagcagg gaaacttcaa gaacctgatg gagttcgtgt ttaagaacat cgatggctac 600
ttcaaaatct actccaagca cacccccatc aacctggtgc gcgacctgcc tcagggcttc 660
tctgccctgg agcccctggt ggatctgcct atcggaatca acatcaccag gtttcagaca 720
ctgctggccc tgcacagaag ctacctgaca ccaggcgact cctctagcgg atggaccgct 780
ggagccgctg cctactacgt gggctacctg cagcctcgga ccttcctgct gaagtacaac 840
gagaacggaa ccatcacaga cgctgtggat tgcgccctgg accccctgtc tgagacaaag 900
tgtacactga agagctttac cgtggagaag ggcatctacc agacaagcaa cttccgggtg 960
cagccaaccg agtccatcgt gcgctttcct aacatcacaa acctgtgccc atttggagag 1020
gtgttcaacg ctacccgctt cgcctccgtg tacgcttgga accggaagcg catctccaac 1080
tgcgtggccg actactctgt gctgtacaac tccgcttctt tcagcacctt taagtgctac 1140
ggcgtgagcc caacaaagct gaacgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcaggg gagacgaggt gcgccagatc gctcctggac agacaggaaa gatcgctgac 1260
tacaactaca agctgccaga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaac 1320
ctggatagca aagtgggcgg aaactacaac tacctgtaca ggctgtttag aaagtctaac 1380
ctgaagccct tcgagaggga catctccaca gaaatctacc aggccggctc taccccttgc 1440
aacggcgtga agggcttcaa ctgttacttc ccactgcaga gctacggatt ccagcccaca 1500
aacggcgtgg gataccagcc ttacagagtg gtggtgctgt cttttgagct gctgcacgct 1560
ccagctacag tgtgcggacc caagaagagc accaacctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttta acggactgac cggcacagga gtgctgaccg agtctaacaa gaagttcctg 1680
ccatttcagc agttcggccg ggacatcgcc gataccacag acgctgtgcg cgacccacag 1740
accctggaga tcctggacat cacaccctgc tccttcggcg gagtgtctgt gatcacaccc 1800
ggcaccaaca caagcaacca ggtggccgtg ctgtaccagg gagtgaactg taccgaggtg 1860
cctgtggcta tccacgccga ccagctgacc ccaacatgga gggtgtacag caccggctcc 1920
aacgtgttcc agacaagagc cggctgtctg atcggagctg agcacgtgaa caacagctac 1980
gagtgcgaca tcccaatcgg cgccggaatc tgtgcttcct accagaccca gacaaactcc 2040
cccaggagag ccaggtctgt ggcttcccag tctatcatcg cctacaccat gagcctgggc 2100
gccgagaact ccgtggctta ctctaacaac agcatcgcta tcccaaccaa cttcacaatc 2160
tccgtgacca cagagatcct gcccgtgagc atgaccaaga catccgtgga ctgcacaatg 2220
tacatctgtg gagattccac cgagtgctct aacctgctgc tgcagtacgg ctctttttgt 2280
acccagctga acagagccct gacaggaatc gctgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca aatctacaag acccccccta tcaaggactt tggcggattc 2400
aacttttccc agatcctgcc tgatccatcc aagccctcta agcggagctt tatcgaggac 2460
ctgctgttca acaaggtgac cctggctgat gccggcttca tcaagcagta cggcgattgc 2520
ctgggagaca tcgctgcccg cgacctgatc tgtgcccaga agtttaacgg actgaccgtg 2580
ctgccacccc tgctgacaga tgagatgatc gctcagtaca catctgctct gctggccggc 2640
accatcacaa gcggatggac cttcggcgct ggagctgccc tgcagatccc ttttgccatg 2700
cagatggctt accggttcaa cggcatcgga gtgacccaga acgtgctgta cgagaaccag 2760
aagctgatcg ccaaccagtt taactccgct atcggcaaga tccaggactc tctgtcctct 2820
acagccagcg ccctgggaaa gctgcaggat gtggtgaacc agaacgctca ggccctgaac 2880
accctggtga agcagctgag cagcaacttc ggcgccatct ctagcgtgct gaacgacatc 2940
ctgagcaggc tggacaaggt ggaggctgag gtgcagatcg acaggctgat cacaggaaga 3000
ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggctgctga gatcagggct 3060
tctgccaacc tggctgccac caagatgagc gagtgcgtgc tgggccagtc caagagagtg 3120
gacttttgtg gcaagggata ccacctgatg agcttccctc agtccgcccc acacggagtg 3180
gtgtttctgc acgtgaccta cgtgcccgct caggagaaga acttcaccac agctcctgcc 3240
atctgccacg acggcaaggc ccactttcca cgggagggcg tgttcgtgag caacggaacc 3300
cactggtttg tgacacagcg caacttctac gagccccaga tcatcaccac agacaacaca 3360
ttcgtgtccg gcaactgtga cgtggtcatc ggaatcgtga acaacaccgt gtacgatcca 3420
ctgcagcccg agctggactc ttttaaggag gagctggata agtacttcaa gaaccacaca 3480
agccctgacg tggatctggg cgacatctct ggaatcaacg ccagcttcgt gaacatccag 3540
aaggagatcg acagactgaa cgaggtggct aagaacctga acgagagcct gatcgatctg 3600
caggagctgg gcaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttc 3660
atcgccggac tgatcgctat cgtgatggtg accatcatgc tgtgttgtat gacctcctgt 3720
tgctcttgcc tgaagggatg ctgttcttgc ggtagttgtt gcaagtttga tgaagatgat 3780
tctgaacccg tgctcaaggg cgtaaagctc cactatacat ga                    3822


<210>	4
<211>	3762 
<212>	DNA
<213>	Severe acute respiratory syndrome coronavirus 2
<220>	
	
<223>	Incomplete cDNA of the surface glycoprotein (Spike protein-Δ63), codon optimized for Mouse. 63 base pair nucleotides removed from the C-terminal region.

<400>   4
	
atgttcgtct ttctggtgct cctgcctctc gtgtcatcac agtgcgtcaa tctcacaact 60
cggactcagc tcccccctgc ttatacaaac agcttcacaa ggggcgtgta ctaccctgac 120
aaggtgttta gaagctccgt gctgcactcc acacaggatc tgtttctgcc attcttttct 180
aacgtgacct ggttccacgc tatccacgtg tccggcacca acggaacaaa gaggttcgac 240
aaccccgtgc tgccttttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc 300
atcagaggct ggatctttgg aaccacactg gacagcaaga cacagtccct gctgatcgtg 360
aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa cgatcccttc 420
ctgggcgtgt actaccacaa gaacaacaag tcttggatgg agagcgagtt tcgggtgtac 480
tctagcgcca acaactgcac atttgagtac gtgtcccagc ctttcctgat ggacctggag 540
ggcaagcagg gaaacttcaa gaacctgatg gagttcgtgt ttaagaacat cgatggctac 600
ttcaaaatct actccaagca cacccccatc aacctggtgc gcgacctgcc tcagggcttc 660
tctgccctgg agcccctggt ggatctgcct atcggaatca acatcaccag gtttcagaca 720
ctgctggccc tgcacagaag ctacctgaca ccaggcgact cctctagcgg atggaccgct 780
ggagccgctg cctactacgt gggctacctg cagcctcgga ccttcctgct gaagtacaac 840
gagaacggaa ccatcacaga cgctgtggat tgcgccctgg accccctgtc tgagacaaag 900
tgtacactga agagctttac cgtggagaag ggcatctacc agacaagcaa cttccgggtg 960
cagccaaccg agtccatcgt gcgctttcct aacatcacaa acctgtgccc atttggagag 1020
gtgttcaacg ctacccgctt cgcctccgtg tacgcttgga accggaagcg catctccaac 1080
tgcgtggccg actactctgt gctgtacaac tccgcttctt tcagcacctt taagtgctac 1140
ggcgtgagcc caacaaagct gaacgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcaggg gagacgaggt gcgccagatc gctcctggac agacaggaaa gatcgctgac 1260
tacaactaca agctgccaga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaac 1320
ctggatagca aagtgggcgg aaactacaac tacctgtaca ggctgtttag aaagtctaac 1380
ctgaagccct tcgagaggga catctccaca gaaatctacc aggccggctc taccccttgc 1440
aacggcgtga agggcttcaa ctgttacttc ccactgcaga gctacggatt ccagcccaca 1500
aacggcgtgg gataccagcc ttacagagtg gtggtgctgt cttttgagct gctgcacgct 1560
ccagctacag tgtgcggacc caagaagagc accaacctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttta acggactgac cggcacagga gtgctgaccg agtctaacaa gaagttcctg 1680
ccatttcagc agttcggccg ggacatcgcc gataccacag acgctgtgcg cgacccacag 1740
accctggaga tcctggacat cacaccctgc tccttcggcg gagtgtctgt gatcacaccc 1800
ggcaccaaca caagcaacca ggtggccgtg ctgtaccagg gagtgaactg taccgaggtg 1860
cctgtggcta tccacgccga ccagctgacc ccaacatgga gggtgtacag caccggctcc 1920
aacgtgttcc agacaagagc cggctgtctg atcggagctg agcacgtgaa caacagctac 1980
gagtgcgaca tcccaatcgg cgccggaatc tgtgcttcct accagaccca gacaaactcc 2040
cccaggagag ccaggtctgt ggcttcccag tctatcatcg cctacaccat gagcctgggc 2100
gccgagaact ccgtggctta ctctaacaac agcatcgcta tcccaaccaa cttcacaatc 1160
tccgtgacca cagagatcct gcccgtgagc atgaccaaga catccgtgga ctgcacaatg 2220
tacatctgtg gagattccac cgagtgctct aacctgctgc tgcagtacgg ctctttttgt 2280
acccagctga acagagccct gacaggaatc gctgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca aatctacaag acccccccta tcaaggactt tggcggattc 2400
aacttttccc agatcctgcc tgatccatcc aagccctcta agcggagctt tatcgaggac 2460
ctgctgttca acaaggtgac cctggctgat gccggcttca tcaagcagta cggcgattgc 2520
ctgggagaca tcgctgcccg cgacctgatc tgtgcccaga agtttaacgg actgaccgtg 2580
ctgccacccc tgctgacaga tgagatgatc gctcagtaca catctgctct gctggccggc 2640
accatcacaa gcggatggac cttcggcgct ggagctgccc tgcagatccc ttttgccatg 2700
cagatggctt accggttcaa cggcatcgga gtgacccaga acgtgctgta cgagaaccag 2760
aagctgatcg ccaaccagtt taactccgct atcggcaaga tccaggactc tctgtcctct 2820
acagccagcg ccctgggaaa gctgcaggat gtggtgaacc agaacgctca ggccctgaac 2880
accctggtga agcagctgag cagcaacttc ggcgccatct ctagcgtgct gaacgacatc 2940
ctgagcaggc tggacaaggt ggaggctgag gtgcagatcg acaggctgat cacaggaaga 3000
ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggctgctga gatcagggct 3060
tctgccaacc tggctgccac caagatgagc gagtgcgtgc tgggccagtc caagagagtg 3120
gacttttgtg gcaagggata ccacctgatg agcttccctc agtccgcccc acacggagtg 3180
gtgtttctgc acgtgaccta cgtgcccgct caggagaaga acttcaccac agctcctgcc 3240
atctgccacg acggcaaggc ccactttcca cgggagggcg tgttcgtgag caacggaacc 3300
cactggtttg tgacacagcg caacttctac gagccccaga tcatcaccac agacaacaca 3360
ttcgtgtccg gcaactgtga cgtggtcatc ggaatcgtga acaacaccgt gtacgatcca 3420
ctgcagcccg agctggactc ttttaaggag gagctggata agtacttcaa gaaccacaca 3480
agccctgacg tggatctggg cgacatctct ggaatcaacg ccagcttcgt gaacatccag 3540
aaggagatcg acagactgaa cgaggtggct aagaacctga acgagagcct gatcgatctg 3600
caggagctgg gcaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttc 3660
atcgccggac tgatcgctat cgtgatggtg accatcatgc tgtgttgtat gacctcctgt 3720
tgctcttgcc tgaagggatg ctgttcttgc ggtagttgat aa                    3762


