                         SEQUENCE LISTING

<110>  Zhong, Minghong
 
<120>  Segmented Nucleic Acids

<130>  01-00001

<150>  US 63284025
<151>  2021-11-30

<160>  45    

<170>  PatentIn version 3.5

<210>  1
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3' Azido crgRNA, truncated. a synthetic oligonucleotide.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  1
nnnnnnnnnn nnnnnnnnnn guuuuagagc ua                                     32


<210>  2
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-alkynyl 3'-amino tracrg1RNA, a synthetic oligonucleotide.

<400>  2
uagcaaguua aaauaaggcu aguccguuau caacuu                                 36


<210>  3
<211>  68
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-Amino RNA segment comprising crgRNA and tracrg1RNA joined by a
       nNt-linker between a32 and u33.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  3
nnnnnnnnnn nnnnnnnnnn guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuu                                                                68


<210>  4
<211>  68
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-Azido RNA segment comprising crgRNA and tracrg1RNA joined by a
       nNt-linker between a32 and u33.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  4
nnnnnnnnnn nnnnnnnnnn guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuu                                                                68


<210>  5
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-cyclooctynyl tracrg2RNA, a synthetic oligonucleotide.

<400>  5
aaguggcacc gagucggugc uu                                                22


<210>  6
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  

       l2gRNA joined by two non-nucleotide linkers between a32 and u33, 
       and u68 and a69, respectively.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  6
nnnnnnnnnn nnnnnnnnnn guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuaa guggcaccga gucggugcuu                                        90


<210>  7
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-alkynyl tracrg2RNA, a synthetic oligonucleotide.

<400>  7
aaguggcacc gagucggugc uu                                                22


<210>  8
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  l2gRNA joined by two non-nucleotide linkers between a32 and u33, 
       and u68 and a69, respectively.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  8
nnnnnnnnnn nnnnnnnnnn guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuaa guggcaccga gucggugcuu                                        90


<210>  9
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-triphenylphosphine l tracrg2RNA, a synthetic oligonucleotide.

<400>  9
aaguggcacc gagucggugc uu                                                22


<210>  10
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  l2gRNA joined by two non-nucleotide linkers between a32 and u33, 
       and u68 and a69, respectively.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  10
nnnnnnnnnn nnnnnnnnnn guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuaa guggcaccga gucggugcuu                                        90


<210>  11
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-alkynyl, 3'-amino tracrg2RNA, a synthetic oligonucleotide.

<400>  11
aaguggcacc gagucggugc uu                                                22


<210>  12
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-amino l2gRNA joined by two non-nucleotide linkers between a32 
       and u33, and u68 and a69, respectively.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  12
nnnnnnnnnn nnnnnnnnnn guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuaa guggcaccga gucggugcuu                                        90


<210>  13
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-conjugates of l2gRNA joined by two non-nucleotide linkers 
       between a32 and u33, and u68 and a69, respectively.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  13
nnnnnnnnnn nnnnnnnnnn guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuaa guggcaccga gucggugcuu                                        90


<210>  14
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-Amino, 3'-alkynyl crgRNA, truncated, a synthetic 
       oligonucleotide.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  14
nnnnnnnnnn nnnnnnnnnn guuuuagagc ua                                     32


<210>  15
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-Amino 3'-azido tracrg1RNA, a synthetic oligonucleotide.

<400>  15
uagcaaguua aaauaaggcu aguccguuau caacuu                                 36


<210>  16
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-alkynyl tracrg2RNA, a synthetic oligonucleotide.

<400>  16
aaguggcacc gagucggugc uut                                               23


<210>  17
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-amino l-tracrgRNA comprising a nNt-linker between u36 and a37,
       a synthetic oligonucleotide.

<400>  17
uagcaaguua aaauaaggcu aguccguuau caacuuaagu ggcaccgagu cggugcuuc        59


<210>  18
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-azido l-tracrgRNA comprising a nNt-linker between u36 and a37,
       a synthetic oligonucleotide.

<400>  18
uagcaaguua aaauaaggcu aguccguuau caacuuaagu ggcaccgagu cggugcuuc        59


<210>  19
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  l2gRNA joined by two non-nucleotide linkers between a32 and u33, 
       and u68 and a69, respectively.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, t or u

<400>  19
nnnnnnnnnn nnnnnnnnnn guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuaa guggcaccga gucggugcuu c                                      91


<210>  20
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  segRNA joined by two non-nucleotide linkers between a32 and u33, 
       and u68 and a69, respectively.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (91)..(120)
<223>  n is a, c, g, t or u

<400>  20
nnnnnnnnnn nnnnnnnnnn guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuaa guggcaccga gucggugcuu nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn      120


<210>  21
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Complementary sequence of ssDNA adaptor in segRNA of Sequence No.
       20..


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  n is a, c, g, or t

<400>  21
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn                                        30


<210>  22
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  segRNA joined by two non-nucleotide linkers between a32 and u33, 
       and u68 and a69, respectively. Only one strand of dsDNA adaptor 
       segment is shown.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (91)..(120)
<223>  n is a, c, g, t or u

<400>  22
nnnnnnnnnn nnnnnnnnnn guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuaa guggcaccga gucggugcuu nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn      120


<210>  23
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  segRNA joined by two non-nucleotide linkers between a32 and u33, 
       and u68 and a69, respectively. The dsDNA is processed with an 
       endouclease to give a sticky end. Only the covalently joined 
       strand of dsDNA adaptro is shown.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (91)..(103)
<223>  n is a, c, g, t or u

<400>  23
nnnnnnnnnn nnnnnnnnnn guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuaa guggcaccga gucggugcuu nnnnnnnnnn nnn                        103


<210>  24
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  segRNA joined by two non-nucleotide linkers between a32 and u33, 
       and u68 and a69, respectively. The dsDNA is a gene editing 
       template. Only the covalently joined strand of the dsDNA template
       is shown. Incomplete sequence.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (91)..(147)
<223>  n is a, c, g, t or u

<400>  24
nnnnnnnnnn nnnnnnnnnn guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuaa guggcaccga gucggugcuu nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn      120

nnnnnnnnnn nnnnnnnnnn nnnnnnn                                          147


<210>  25
<211>  115
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  segRNA joined by two non-nucleotide linkers between a32 and u33, 
       and u68 and a69, respectively. The ssDNA hybridized with the 
       adaptor is a gene editing template. Only the covalently joined 
       strand of ssDNA adaptor segment is shown. Incomplete sequence.


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (91)..(115)
<223>  n is a, c, g, t or u

<400>  25
nnnnnnnnnn nnnnnnnnnn guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuaa guggcaccga gucggugcuu nnnnnnnnnn nnnnnnnnnn nnnnn           115


<210>  26
<211>  44
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  A two-segment nucleic acid comprising ADAR guide RNA's 
       specificity domain and 3'-segment of its recruiting domain, 
       joined by a nNt-linker.


<220>
<221>  misc_feature
<222>  (27)..(44)
<223>  n is a, c, g, or u

<400>  26
aauguuguuc ucgucuccuc gacaccnnnn nnnnnnnnnn nnnn                        44


<210>  27
<211>  69
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  A three-segment ADAR guide RNA joined by two nNt-linkers between 
       u23 and a24, and c51 and n52, respectively.


<220>
<221>  misc_feature
<222>  (52)..(69)
<223>  n is a, c, g, or u

<400>  27
ggugucgaga agaggagaac aauauaaugu uguucucguc uccucgacac cnnnnnnnnn       60

nnnnnnnnn                                                               69


<210>  28
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3' Azido crgRNA, truncated, a synthetic oligonucleotide targeting
       eGFP.

<400>  28
cucgugacca cccugaccua guuuuagagc ua                                     32


<210>  29
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-alkynyl tracrgRNA.

<400>  29
uagcaaguua aaauaaggcu aguccguuau caacuugaaa aaguggcacc gagucggugc       60

uuut                                                                    64


<210>  30
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  lgRNA targeting eGFP and joined by a non-nucleotide linker 
       between a32 and u33.

<400>  30
cucgugacca cccugaccua guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuga aaaaguggca ccgagucggu gcuuut                                 96


<210>  31
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-Amino, 3' azido crgRNA, truncated, a synthetic oligonucleotide
       targeting eGFP.

<400>  31
cucgugacca cccugaccua guuuuagagc ua                                     32


<210>  32
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-Amino lgRNA targeting eGFP and joined by a non-nucleotide 
       linkers between a32 and u33.

<400>  32
cucgugacca cccugaccua guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuga aaaaguggca ccgagucggu gcuuut                                 96


<210>  33
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-Azido lgRNA targeting eGFP and joined by a non-nucleotide 
       linker between a32 and u33.

<400>  33
cucgugacca cccugaccua guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuga aaaaguggca ccgagucggu gcuuut                                 96


<210>  34
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-Alkynyl ssDNA template for eGFP --> BFP editing.

<400>  34
ctggcccacc ctcgtgacca ccctgagcca cggggtgcag tgcttcagcc g                51


<210>  35
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  segRNA (eGFP) joined by two nNT-linkers, between c1 and c52, and 
       a83 and u84, respectively. There is no bond between g51 and c52.

<400>  35
ctggcccacc ctcgtgacca ccctgagcca cggggtgcag tgcttcagcc gcucgugacc       60

acccugaccu aguuuuagag cuauagcaag uuaaaauaag gcuaguccgu uaucaacuug      120

aaaaaguggc accgagucgg ugcuuut                                          147


<210>  36
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  segRNA (eGFP) joined by two nNT-linkers, between c1 and c52, and 
       a83 and u84, respectively. There is no bond between g51 and c52.

<400>  36
cggctgaagc actgcacccc gtggctcagg gtggtcacga gggtgggcca gcucgugacc       60

acccugaccu aguuuuagag cuauagcaag uuaaaauaag gcuaguccgu uaucaacuug      120

aaaaaguggc accgagucgg ugcuuut                                          147


<210>  37
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-alkynyl 3'-amino tracrg1RNA, a synthetic oligonucleotide.

<400>  37
uagcaaguua aaauaaggcu aguccguuau caacuu                                 36


<210>  38
<211>  68
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-Amino RNA segment comprising crRNA (eGFP) and tracrg1RNA 
       joined by a nNt-linker between a32 and u33.

<400>  38
cucgugacca cccugaccua guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuu                                                                68


<210>  39
<211>  68
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-Azido RNA segment comprising crRNA (eGFP) and tracrg1RNA 
       joined by a nNt-linker between a32 and u33.

<400>  39
cucgugacca cccugaccua guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuu                                                                68


<210>  40
<211>  68
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-Azido RNA segment comprising crgRNA (eGFP) and tracrg1RNA 
       joined by a nNt-linker between a32 and u33.

<400>  40
cucgugacca cccugaccua guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuu                                                                68


<210>  41
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-alkynyl tracrg2RNA, a synthetic oligonucleotide.

<400>  41
aaguggcacc gagucggugc uuc                                               23


<210>  42
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  l2gRNA targeting eGFP, joined by two non-nucleotide linkers 
       between a32 and u33, and u68 and a69, respectively.

<400>  42
cucgugacca cccugaccua guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuaa guggcaccga gucggugcuu c                                      91


<210>  43
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-Alkynyl tracrg2RNA-ssDNA (eGFP).

<400>  43
aaguggcacc gagucggugc uuuctggccc accctcgtga ccaccctgag ccacggggtg       60

cagtgcttca gccg                                                         74


<210>  44
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  segRNA targeting sGFP, joined by two non-nucleotide linkers 
       between a32 and u33, and u68 and a69, respectively.

<400>  44
cucgugacca cccugaccua guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuaa guggcaccga gucggugcuu uctggcccac cctcgtgacc accctgagcc      120

acggggtgca gtgcttcagc cg                                               142


<210>  45
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  segRNA targeting sGFP, joined by two non-nucleotide linkers 
       between a32 and u33, and u68 and a69, respectively.

<400>  45
cucgugacca cccugaccua guuuuagagc uauagcaagu uaaaauaagg cuaguccguu       60

aucaacuuaa guggcaccga gucggugcuu ucggctgaag cactgcaccc cgtggctcag      120

ggtggtcacg agggtgggcc ag                                               142


