﻿                         序列表

<110>  绍兴守仁医疗健康科技有限公司

<120>  人PAK1蛋白的真核表达载体的构建方法、表达和纯化方法

<160>  4

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  人工序列

<400>  1
agctcaacta tgatcttttt                                                    20

<210>  2
<211>  20
<212>  DNA
<213>  人工序列

<400>  2
gaatattttc atctcctttt                                                     20

<210>  3
<211>  3266
<212>  DNA
<213>  人工序列

<400>  3
agctcaacta tgatcttttt tgagaaacct cggatggtct gagttctacc agttctgcta        60

ctcattagtg aaagaaaaca taatacatgt ctggctgtga gatcacacga catcggatag       120

ttttcaaatt cagcccttga caacgagctc atagatgcgg tggtgctttg ttaacctgaa       180

aaggaccctc agcgccggaa gttagccgtc tttctccatc ctgactacta cagtagccct       240

cccagaaatc gtcccaaaat tgtctgatag aaaggctgca cggaaacaac ccgccacgga       300

cagcggaccg agaacagtga cgcgacgcga tgacgtcacg cagcaagcgg tagctgctgc       360

tggtggtgac aatgtcaaat aacggcctag acattcaaga caaaccccca gcccctccga       420

tgagaaatac cagcactatg attggagccg gcagcaaaga tgctggaacc ctaaaccatg       480

gttctaaacc tctgcctcca aacccagagg agaagaaaaa gaaggaccga ttttaccgat       540

ccattttacc tggagataaa acaaataaaa agaaagagaa agagcggcca gagatttctc       600

tcccttcaga ttttgaacac acaattcatg tcggttttga tgctgtcaca ggggagttta       660

cgggaatgcc agagcagtgg gcccgcttgc ttcagacatc aaatatcact aagtcggagc       720

agaagaaaaa cccgcaggct gttctggatg tgttggagtt ttacaactcg aagaagacat       780

ccaacagcca gaaatacatg agctttacag ataagtcagc tgaggattac aattcttcta       840

atgccttgaa tgtgaaggct gtgtctgaga ctcctgcagt gccaccagtt tcagaagatg       900

aggatgatga tgatgatgat gctaccccac caccagtgat tgctccacgc ccagagcaca       960

caaaatctgt atacacacgg tctgtgattg aaccacttcc tgtcactcca actcgggacg      1020

tggctacatc tcccatttca cctactgaaa ataacaccac tccaccagat gctttgaccc      1080

ggaatactga gaagcagaag aagaagccta aaatgtctga tgaggagatc ttggagaaat      1140

tacgaagcat agtgagtgtg ggcgatccta agaagaaata tacacggttt gagaagattg      1200

gacaaggtgc ttcaggcacc gtgtacacag caatggatgt ggccacagga caggaggtgg      1260

ccattaagca gatgaatctt cagcagcagc ccaagaaaga gctgattatt aatgagatcc      1320

tggtcatgag ggaaaacaag aacccaaaca ttgtgaatta cttggacagt tacctcgtgg      1380

gagatgagct gtgggttgtt atggaatact tggctggagg ctccttgaca gatgtggtga      1440

cagaaacttg catggatgaa ggccaaattg cagctgtgtg ccgtgagtgt ctgcaggctc      1500

tggagttctt gcattcgaac caggtcattc acagagacat caagagtgac aatattctgt      1560

tgggaatgga tggctctgtc aagctaactg actttggatt ctgtgcacag ataaccccag      1620

agcagagcaa acggagcacc atggtaggaa ccccatactg gatggcacca gaggttgtga      1680

cacgaaaggc ctatgggccc aaggttgaca tctggtccct gggcatcatg gccatcgaaa      1740

tgattgaagg ggagcctcca tacctcaatg aaaaccctct gagagccttg tacctcattg      1800

ccaccaatgg gaccccagaa cttcagaacc cagagaagct gtcagctatc ttccgggact      1860

ttctgaaccg ctgtctcgag atggatgtgg agaagagagg ttcagctaaa gagctgctac      1920

agcatcaatt cctgaagatt gccaagcccc tctccagcct cactccactg attgctgcag      1980

ctaaggaggc aacaaagaac aatcactaaa accacactca ccccagcctc attgtgccaa      2040

gccttctgtg agataaatgc acatttcaga aattccaact cctgatgccc tcttctcctt      2100

gccttgcttc tcccatttcc tgatctagca ctcctcaaga ctttgatcct tggaaaccgt      2160

gtgtccagca ttgaagagaa ctgcaactga atgactaatc agatgatggc catttctaaa      2220

taaggaattt cctcccaatt catggatatg agggtggttt atgattaagg gtttatataa      2280

ataaatgttt ctagtcttcc gtgtgtcaaa atcctcacct ccttcataac catctcccac      2340

aattaattct tgactatata aatttatggt ttgataatat tatcaatttg taatcaattg      2400

agatttcttt agtgcttgct tttctgtgac tcaactgccc agacacctca ttgtacttga      2460

aaactggaac agcttgggaa tgccatgggg tttgataatc tgccagggac atgaagaggc      2520

tcagcttcct ggaccatgac tttggctcag ctgatcctga catgggagaa caaccacatt      2580

tttctttgtg tgtgcttcta gcagctgttc gggaggacct tgacccaata gtgttcccat      2640

gctgtttctt gtgaaatgct ctcggctatg tagcagcttt tgattccctg cataccctag      2700

gctgctgccc ctatcctgtc ccttgtttat aacattgaga ggttttctag ggcacatact      2760

gagtgagagc agtgttgaga agtcggggaa aatggtgact acttttagag caaggctggg      2820

catcagcacc tgtccagctc tacttgtgtg atgtttcagg aactcagccc ctttttctgc      2880

ctaggataag gagctgaaag attaacttgg atcttctaat ggtccaaatc ttttggtcac      2940

aataaagagt ctccaaatta gagactgcat gttagttctg gatggatttg gtggcctgac      3000

atgataccct gccagctgtg aggggacccc gtttttaaga tgcatggcca agctctctgc      3060

aaatggaaat gcttacactg ggtgttgggg atgtttgcta cctcctgcta tttttgtggt      3120

tttggttctc ccactatggt aggacccctg gccagcattg tggcttgtca tgtcagcccc      3180

attgactacc ttctcatgct ctgaggtact actgcctctg cagcacaaat ttctatttct      3240

gtcaataaaa ggagatgaaa atattc                                           3266

<210>  4
<211>  3266
<212>  DNA
<213>  人工序列

<400>  4
agctcaacta tgatcttttt tgagaaacct cggatggtct gagttctacc agttctgcta        60

ctcattagtg aaagaaaaca taatacatgt ctggctgtga gatcacacga catcggatag       120

ttttcaaatt cagcccttga caacgagctc atagatgcgg tggtgctttg ttaacctgaa       180

aaggaccctc agcgccggaa gttagccgtc tttctccatc ctgactacta cagtagccct       240

cccagaaatc gtcccaaaat tgtctgatag aaaggctgca cggaaacaac ccgccacgga       300

cagcggaccg agaacagtga cgcgacgcga tgacgtcacg cagcaagcgg tagctgctgc       360

tggtggtgac aatgtcaaat aacggcctag acattcaaga caaaccccca gcccctccga       420

tgagaaatac cagcactatg attggagccg gcagcaaaga tgctggaacc ctaaaccatg       480

gttctaaacc tctgcctcca aacccagagg agaagaaaaa gaaggaccga ttttaccgat       540

ccattttacc tggagataaa acaaataaaa agaaagagaa agagcggcca gagatttctc       600

tcccttcaga ttttgaacac acaattcatg tcggttttga tgctgtcaca ggggagttta       660

cgggaatgcc agagcagtgg gcccgcttgc ttcagacatc aaatatcact aagtcggagc       720

agaagaaaaa cccgcaggct gttctggatg tgttggagtt ttacaactcg aagaagacat       780

ccaacagcca gaaatacatg agctttacag ataagtcagc tgaggattac aattcttcta       840

atgccttgaa tgtgaaggct gtgtctgaga ctcctgcagt gccaccagtt tcagaagatg       900

aggatgatga tgatgatgat gctaccccac caccagtgat tgctccacgc ccagagcaca       960

caaaatctgt atacacacgg tctgtgattg aaccacttcc tgtcactcca actcgggacg      1020

tggctacatc tcccatttca cctactgaaa ataacaccac tccaccagat gctttgaccc      1080

ggaatactga gaagcagaag aagaagccta aaatgtctga tgaggagatc ttggagaaat      1140

tacgaagcat agtgagtgtg ggcgatccta agaagaaata tacacggttt gagaagattg      1200

gacaaggtgc ttcaggcacc gtgtacacag caatggatgt ggccacagga caggaggtgg      1260

ccattaagca gatgaatctt cagcagcagc ccaagaaaga gctgattatt aatgagatcc      1320

tggtcatgag ggaaaacaag aacccaaaca ttgtgaatta cttggacagt tacctcgtgg      1380

gagatgagct gtgggttgtt atggaatact tggctggagg ctccttgaca gatgtggtga      1440

cagaaacttg catggatgaa ggccaaattg cagctgtgtg ccgtgagtgt ctgcaggctc      1500

tggagttctt gcattcgaac caggtcattc acagagacat caagagtgac aatattctgt      1560

tgggaatgga tggctctgtc aagctaactg actttggatt ctgtgcacag ataaccccag      1620

agcagagcaa acggagcacc atggtaggaa ccccatactg gatggcacca gaggttgtga      1680

cacgaaaggc ctatgggccc aaggttgaca tctggtccct gggcatcatg gccatcgaaa      1740

tgattgaagg ggagcctcca tacctcaatg aaaaccctct gagagccttg tacctcattg      1800

ccaccaatgg gaccccagaa cttcagaacc cagagaagct gtcagctatc ttccgggact      1860

ttctgaaccg ctgtctcgag atggatgtgg agaagagagg ttcagctaaa gagctgctac      1920

agcatcaatt cctgaagatt gccaagcccc tctccagcct cactccactg attgctgcag      1980

ctaaggaggc aacaaagaac aatcactaaa accacactca ccccagcctc attgtgccaa      2040

gccttctgtg agataaatgc acatttcaga aattccaact cctgatgccc tcttctcctt      2100

gccttgcttc tcccatttcc tgatctagca ctcctcaaga ctttgatcct tggaaaccgt      2160

gtgtccagca ttgaagagaa ctgcaactga atgactaatc agatgatggc catttctaaa      2220

taaggaattt cctcccaatt catggatatg agggtggttt atgattaagg gtttatataa      2280

ataaatgttt ctagtcttcc gtgtgtcaaa atcctcacct ccttcataac catctcccac      2340

aattaattct tgactatata aatttatggt ttgataatat tatcaatttg taatcaattg      2400

agatttcttt agtgcttgct tttctgtgac tcaactgccc agacacctca ttgtacttga      2460

aaactggaac agcttgggaa tgccatgggg tttgataatc tgccagggac atgaagaggc      2520

tcagcttcct ggaccatgac tttggctcag ctgatcctga catgggagaa caaccacatt      2580

tttctttgtg tgtgcttcta gcagctgttc gggaggacct tgacccaata gtgttcccat      2640

gctgtttctt gtgaaatgct ctcggctatg tagcagcttt tgattccctg cataccctag      2700

gctgctgccc ctatcctgtc ccttgtttat aacattgaga ggttttctag ggcacatact      2760

gagtgagagc agtgttgaga agtcggggaa aatggtgact acttttagag caaggctggg      2820

catcagcacc tgtccagctc tacttgtgtg atgtttcagg aactcagccc ctttttctgc      2880

ctaggataag gagctgaaag attaacttgg atcttctaat ggtccaaatc ttttggtcac      2940

aataaagagt ctccaaatta gagactgcat gttagttctg gatggatttg gtggcctgac      3000

atgataccct gccagctgtg aggggacccc gtttttaaga tgcatggcca agctctctgc      3060

aaatggaaat gcttacactg ggtgttgggg atgtttgcta cctcctgcta tttttgtggt      3120

tttggttctc ccactatggt aggacccctg gccagcattg tggcttgtca tgtcagcccc      3180

attgactacc ttctcatgct ctgaggtact actgcctctg cagcacaaat ttctatttct      3240

gtcaataaaa ggagatgaaa atattc                                           3266



