﻿                         序列表

<110>  京东方科技集团股份有限公司
       成都京东方光电科技有限公司

<120>  DNA末端修复、连接接头试剂、试剂盒及DNA文库构建方法

<130>  214553PCT-JDF/Z

<160>  3

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  301
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  1
Met Phe Lys Arg Lys Ser Thr Ala Glu Leu Ala Ala Gln Met Ala Lys 
1               5                   10                  15      
Leu Asn Gly Asn Lys Gly Phe Ser Ser Glu Asp Lys Gly Glu Trp Lys 
            20                  25                  30          
Leu Lys Leu Asp Asn Ala Gly Asn Gly Gln Ala Val Ile Arg Phe Leu 
        35                  40                  45              
Pro Ser Lys Asn Asp Glu Gln Ala Pro Phe Ala Ile Leu Val Asn His 
    50                  55                  60                  
Gly Phe Lys Lys Asn Gly Lys Trp Tyr Ile Glu Thr Cys Ser Ser Thr 
65                  70                  75                  80  
His Gly Asp Tyr Asp Ser Cys Pro Val Cys Gln Tyr Ile Ser Lys Asn 
                85                  90                  95      
Asp Leu Tyr Asn Thr Asp Asn Lys Glu Tyr Ser Leu Val Lys Arg Lys 
            100                 105                 110         
Thr Ser Tyr Trp Ala Asn Ile Leu Val Val Lys Asp Pro Ala Ala Pro 
        115                 120                 125             
Glu Asn Glu Gly Lys Val Phe Lys Tyr Arg Phe Gly Lys Lys Ile Trp 
    130                 135                 140                 
Asp Lys Ile Asn Ala Met Ile Ala Val Asp Val Glu Met Gly Glu Thr 
145                 150                 155                 160 
Pro Val Asp Val Thr Cys Pro Trp Glu Gly Ala Asn Phe Val Leu Lys 
                165                 170                 175     
Val Lys Gln Val Ser Gly Phe Ser Asn Tyr Asp Glu Ser Lys Phe Leu 
            180                 185                 190         
Asn Gln Ser Ala Ile Pro Asn Ile Asp Asp Glu Ser Phe Gln Lys Glu 
        195                 200                 205             
Leu Phe Glu Gln Met Val Asp Leu Ser Glu Met Thr Ser Lys Asp Lys 
    210                 215                 220                 
Phe Lys Ser Phe Glu Glu Leu Asn Thr Lys Phe Gly Gln Val Met Gly 
225                 230                 235                 240 
Thr Ala Val Met Gly Gly Ala Ala Ala Thr Ala Ala Lys Lys Ala Asp 
                245                 250                 255     
Lys Val Ala Asp Asp Leu Asp Ala Phe Asn Val Asp Asp Phe Asn Thr 
            260                 265                 270         
Lys Thr Glu Asp Asp Phe Met Ser Ser Ser Ser Gly Ser Ser Ser Ser 
        275                 280                 285             
Ala Asp Asp Thr Asp Leu Asp Asp Leu Leu Asn Asp Leu 
    290                 295                 300     

<210>  2
<211>  605
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  2
Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu Glu Thr Leu 
1               5                   10                  15      
Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe Ala Phe Asp 
            20                  25                  30          
Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu Val Gly Leu 
        35                  40                  45              
Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro Val Ala His 
    50                  55                  60                  
Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg Ala Leu Glu 
65                  70                  75                  80  
Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys Val Gly Gln 
                85                  90                  95      
Asn Leu Lys Tyr Asp Arg Gly Ile Leu Ala Asn Tyr Gly Ile Glu Leu 
            100                 105                 110         
Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile Leu Asn Ser 
        115                 120                 125             
Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg Trp Leu Lys 
    130                 135                 140                 
His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly Lys Asn Gln 
145                 150                 155                 160 
Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg Tyr Ala Ala 
                165                 170                 175     
Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met Trp Pro Asp 
            180                 185                 190         
Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn Ile Glu Met 
        195                 200                 205             
Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly Val Lys Ile 
    210                 215                 220                 
Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr Leu Arg Leu 
225                 230                 235                 240 
Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu Glu Phe Asn 
                245                 250                 255     
Leu Ser Ser Thr Lys Gln Leu Gln Thr Ile Leu Phe Glu Lys Gln Gly 
            260                 265                 270         
Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser Thr Ser Glu 
        275                 280                 285             
Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro Lys Val Ile 
    290                 295                 300                 
Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr Thr Asp Lys 
305                 310                 315                 320 
Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His Thr Ser Tyr 
                325                 330                 335     
His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr Asp Pro Asn 
            340                 345                 350         
Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg Ile Arg Gln 
        355                 360                 365             
Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala Asp Tyr Ser 
    370                 375                 380                 
Gln Ile Glu Leu Arg Ile Met Ala His Leu Ser Arg Asp Lys Gly Leu 
385                 390                 395                 400 
Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala Thr Ala Ala 
                405                 410                 415     
Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu Gln Arg Arg 
            420                 425                 430         
Ser Ala Lys Ala Ile Asn Tyr Gly Leu Ile Tyr Gly Met Ser Ala Phe 
        435                 440                 445             
Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala Gln Lys Tyr 
    450                 455                 460                 
Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu Tyr Met Glu 
465                 470                 475                 480 
Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu Thr Leu Asp 
                485                 490                 495     
Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn Gly Ala Arg 
            500                 505                 510         
Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met Gln Gly Thr 
        515                 520                 525             
Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp Ala Trp Leu 
    530                 535                 540                 
Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val His Asp Glu 
545                 550                 555                 560 
Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val Ala Lys Gln 
                565                 570                 575     
Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val Pro Leu Leu 
            580                 585                 590         
Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        595                 600                 605 

<210>  3
<211>  605
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  3
Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu Glu Thr Leu 
1               5                   10                  15      
Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe Ala Phe Asp 
            20                  25                  30          
Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu Val Gly Leu 
        35                  40                  45              
Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro Val Ala His 
    50                  55                  60                  
Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg Ala Leu Glu 
65                  70                  75                  80  
Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys Val Gly Gln 
                85                  90                  95      
Asn Leu Lys Tyr Asp Arg Gly Ile Leu Ala Asn Tyr Gly Ile Glu Leu 
            100                 105                 110         
Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile Leu Asn Ser 
        115                 120                 125             
Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg Trp Leu Lys 
    130                 135                 140                 
His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly Lys Asn Gln 
145                 150                 155                 160 
Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg Tyr Ala Ala 
                165                 170                 175     
Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met Trp Pro Asp 
            180                 185                 190         
Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn Ile Glu Met 
        195                 200                 205             
Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly Val Lys Ile 
    210                 215                 220                 
Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr Leu Arg Leu 
225                 230                 235                 240 
Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu Glu Phe Asn 
                245                 250                 255     
Leu Ser Ser Thr Lys Gln Leu Gln Thr Ile Leu Phe Glu Lys Gln Gly 
            260                 265                 270         
Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser Thr Ser Glu 
        275                 280                 285             
Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro Lys Val Ile 
    290                 295                 300                 
Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr Thr Asp Lys 
305                 310                 315                 320 
Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His Thr Ser Tyr 
                325                 330                 335     
His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr Asp Pro Asn 
            340                 345                 350         
Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg Ile Arg Gln 
        355                 360                 365             
Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala Asp Tyr Ser 
    370                 375                 380                 
Gln Ile Glu Leu Arg Ile Met Ala His Leu Ser Arg Asp Lys Gly Leu 
385                 390                 395                 400 
Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala Thr Ala Ala 
                405                 410                 415     
Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu Gln Arg Arg 
            420                 425                 430         
Ser Ala Lys Ala Ile Asn Phe Gly Leu Ile Tyr Gly Met Ser Ala Phe 
        435                 440                 445             
Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala Gln Lys Tyr 
    450                 455                 460                 
Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu Tyr Met Glu 
465                 470                 475                 480 
Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu Thr Leu Asp 
                485                 490                 495     
Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn Gly Ala Arg 
            500                 505                 510         
Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met Gln Gly Thr 
        515                 520                 525             
Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp Ala Trp Leu 
    530                 535                 540                 
Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val His Asp Glu 
545                 550                 555                 560 
Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val Ala Lys Gln 
                565                 570                 575     
Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val Pro Leu Leu 
            580                 585                 590         
Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        595                 600                 605 

