                         SEQUENCE LISTING

<110>  City University of Hong Kong Shenzhen Research Institute
       BGI-Shenzhen
 
<120>  High-affinity anti-EGFP and anti-SARS-CoV-2 vNAR single domain 
       antibodies and use thereof

<130>  WPI21CN0222X

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  114
<212>  PRT
<213>  Chiloscyllium plagiosum

<400>  1

Glu Ser Pro Arg Val Glu Gln Thr Pro Thr Thr Thr Thr Lys Glu Ala 
1               5                   10                  15      


Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Lys Gly Ser Ser Cys Ala 
            20                  25                  30          


Leu Gly Tyr Thr Tyr Trp Tyr Phe Thr Lys Lys Gly Ala Thr Lys Lys 
        35                  40                  45              


Thr Ser Leu Ser Thr Gly Gly Arg Tyr Ser Asp Thr Lys Asn Thr Ala 
    50                  55                  60                  


Ser Lys Ser Phe Ser Leu Arg Ile Ser Asp Leu Arg Val Glu Asp Ser 
65                  70                  75                  80  


Gly Thr Tyr His Cys Glu Ala Tyr Glu Ala Trp Asp Glu Ser Asp Ser 
                85                  90                  95      


Trp Asn Cys Gly Asp Tyr Tyr Glu Gly Gly Gly Thr Val Val Ala Val 
            100                 105                 110         


Lys Pro 
        


<210>  2
<211>  110
<212>  PRT
<213>  Chiloscyllium plagiosum

<400>  2

Glu Thr Pro Arg Val Glu Gln Thr Pro Thr Thr Thr Thr Lys Glu Ala 
1               5                   10                  15      


Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Lys Gly Ser Ile Cys Ala 
            20                  25                  30          


Leu Gly Asn Thr Tyr Trp Tyr Phe Thr Lys Lys Gly Ala Thr Lys Lys 
        35                  40                  45              


Ala Ser Leu Ser Thr Gly Gly Arg Tyr Ser Asp Thr Lys Asn Thr Ala 
    50                  55                  60                  


Ser Lys Ser Phe Ser Leu Arg Ile Ser Asp Leu Arg Val Glu Asp Ser 
65                  70                  75                  80  


Gly Thr Tyr His Cys Glu Ala Tyr Arg Gly Pro Ser Cys Gly Gly Asn 
                85                  90                  95      


Trp Ala Tyr Glu Gly Gly Gly Thr Val Leu Thr Val Asn Pro 
            100                 105                 110 


<210>  3
<211>  112
<212>  PRT
<213>  Chiloscyllium plagiosum

<400>  3

Glu Ser Ala Arg Val Glu Gln Thr Pro Thr Thr Thr Thr Lys Glu Ala 
1               5                   10                  15      


Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Lys Gly Ser Arg Cys Thr 
            20                  25                  30          


Val Asp Arg Thr Tyr Trp Tyr Phe Thr Lys Lys Gly Ala Thr Lys Lys 
        35                  40                  45              


Ala Ser Leu Ser Thr Gly Gly Arg Tyr Ser Asp Thr Lys Asn Thr Ala 
    50                  55                  60                  


Ser Lys Ser Phe Ser Leu Arg Ile Ser Asp Leu Arg Val Glu Asp Ser 
65                  70                  75                  80  


Gly Thr Tyr His Cys Lys Ala Tyr Lys Cys Ser Trp Glu Arg Asn Pro 
                85                  90                  95      


Trp Asp Asp Tyr Tyr Glu Gly Gly Gly Thr Val Val Thr Val Lys Pro 
            100                 105                 110         


<210>  4
<211>  112
<212>  PRT
<213>  Chiloscyllium plagiosum

<400>  4

Glu Ser Ala Arg Val Glu Gln Thr Pro Thr Thr Thr Thr Lys Glu Ala 
1               5                   10                  15      


Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Arg Asp Ser Thr Cys Ala 
            20                  25                  30          


Leu Gly Asn Thr Tyr Trp Tyr Phe Thr Lys Lys Gly Ala Thr Lys Lys 
        35                  40                  45              


Ala Ser Leu Ser Thr Gly Gly Arg Tyr Ala Glu Thr Val Asn Lys Ala 
    50                  55                  60                  


Ser Lys Ser Phe Ser Leu Arg Ile Ser Asp Leu Arg Val Glu Asp Ser 
65                  70                  75                  80  


Gly Thr Tyr Arg Cys Lys Ala Tyr Pro Pro Leu Asp Gly Gly Cys Tyr 
                85                  90                  95      


Thr Ala Asn Ile Ala Glu Gly Gly Gly Thr Val Leu Thr Val Lys Pro 
            100                 105                 110         


<210>  5
<211>  110
<212>  PRT
<213>  Chiloscyllium plagiosum

<400>  5

Glu Thr Ala Arg Val Glu Gln Thr Pro Thr Thr Thr Thr Lys Glu Ala 
1               5                   10                  15      


Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Arg Asp Ser Ser Cys Ala 
            20                  25                  30          


Leu Gly Ser Thr Tyr Trp His Phe Thr Lys Lys Gly Ala Thr Lys Lys 
        35                  40                  45              


Ala Ile Leu Ser Thr Gly Gly Arg Tyr Ala Glu Thr Val Asn Lys Ala 
    50                  55                  60                  


Ser Lys Ser Phe Ser Leu Arg Ile Ser Asp Leu Arg Val Glu Asp Ser 
65                  70                  75                  80  


Gly Thr Tyr His Cys Arg Ala Tyr Pro Gln Met Gly Cys Arg Ser Ala 
                85                  90                  95      


Gly Ile Gly Glu Gly Gly Gly Thr Ala Val Thr Val Asn Pro 
            100                 105                 110 


<210>  6
<211>  110
<212>  PRT
<213>  Chiloscyllium plagiosum

<400>  6

Glu Thr Pro Arg Val Glu Gln Thr Pro Thr Thr Thr Thr Lys Glu Ala 
1               5                   10                  15      


Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Lys Gly Ser Ile Cys Gly 
            20                  25                  30          


Leu Gly Ser Thr Tyr Trp Tyr Phe Thr Lys Lys Gly Ala Thr Lys Lys 
        35                  40                  45              


Ala Thr Leu Ser Thr Gly Gly Arg Tyr Ser Asp Thr Lys Asn Thr Ala 
    50                  55                  60                  


Ser Lys Ser Phe Ser Leu Arg Ile Ser Asp Leu Arg Val Glu Asp Ser 
65                  70                  75                  80  


Gly Thr Tyr His Cys Glu Ala Ser Arg Asp Gln Tyr Cys Gly Gly Arg 
                85                  90                  95      


Trp Val Tyr Glu Gly Gly Gly Thr Ser Leu Thr Val Lys Pro 
            100                 105                 110 


<210>  7
<211>  110
<212>  PRT
<213>  Chiloscyllium plagiosum

<400>  7

Glu Ser Ala Arg Val Glu Gln Thr Pro Thr Thr Thr Thr Lys Glu Ala 
1               5                   10                  15      


Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Lys Gly Ser Ile Cys Gly 
            20                  25                  30          


Leu Gly Arg Thr His Trp Tyr Phe Thr Lys Lys Gly Ala Thr Lys Lys 
        35                  40                  45              


Ala Ser Leu Ser Thr Gly Gly Arg Tyr Ser Asp Thr Lys Asn Thr Ala 
    50                  55                  60                  


Ser Lys Ser Phe Ser Leu Arg Val Ser Asp Leu Arg Ile Glu Asp Ser 
65                  70                  75                  80  


Gly Thr Tyr His Cys Glu Ala Phe Arg Asp Glu Tyr Cys Gly Gly Glu 
                85                  90                  95      


Phe Pro Tyr Glu Gly Gly Gly Thr Thr Leu Thr Val Asn Pro 
            100                 105                 110 


<210>  8
<211>  118
<212>  PRT
<213>  Chiloscyllium plagiosum

<400>  8

Gln Val Gln Leu Gln Glu Thr Gln Arg Val Glu Gln Thr Pro Thr Thr 
1               5                   10                  15      


Thr Thr Lys Glu Ala Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Lys 
            20                  25                  30          


Gly Ser Ser Cys Pro Leu Gly Asn Thr Arg Trp Tyr Phe Thr Lys Lys 
        35                  40                  45              


Gly Ala Thr Lys Lys Ala Asn Leu Ser Thr Gly Gly Arg Tyr Ser Asp 
    50                  55                  60                  


Thr Lys Asn Thr Ala Ser Lys Ser Phe Ser Leu Arg Ile Ser Asp Leu 
65                  70                  75                  80  


Arg Val Glu Asp Ser Gly Thr Tyr His Cys Glu Ala Tyr Thr Cys Trp 
                85                  90                  95      


Asp Ser Gly His Thr Gly Gly Tyr Lys Asp Phe Val Gly Gly Gly Thr 
            100                 105                 110         


Val Leu Ala Val Asn Pro 
        115             


<210>  9
<211>  124
<212>  PRT
<213>  Chiloscyllium plagiosum

<400>  9

Gln Val Gln Leu Gln Glu Thr Ala Arg Val Glu Gln Thr Pro Thr Thr 
1               5                   10                  15      


Thr Thr Lys Glu Ala Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Lys 
            20                  25                  30          


Gly Ser Arg Tyr Glu Leu Cys Ser Ser His Trp Tyr Phe Thr Arg Lys 
        35                  40                  45              


Gly Ala Thr Lys Lys Glu Ser Leu Ser Asn Gly Gly Arg Tyr Ala Glu 
    50                  55                  60                  


Thr Val Asn Lys Ala Ser Lys Ser Phe Ser Leu Arg Ile Ser Asp Leu 
65                  70                  75                  80  


Arg Val Glu Asp Ser Gly Thr Tyr His Cys Glu Ala Tyr Arg Cys Leu 
                85                  90                  95      


Thr Ala Gly Arg Asp Arg Trp Asp Thr Ile Asp Gly Gly Ser Asp Tyr 
            100                 105                 110         


Tyr Glu Gly Gly Gly Thr Ala Val Ala Val Asn Pro 
        115                 120                 


<210>  10
<211>  118
<212>  PRT
<213>  Chiloscyllium plagiosum

<400>  10

Gln Val Gln Leu Gln Glu Ser Pro Arg Val Glu Gln Thr Pro Thr Thr 
1               5                   10                  15      


Thr Thr Lys Lys Ala Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Lys 
            20                  25                  30          


Gly Ser Ser Cys Ala Leu Gly Ser Thr Tyr Trp Tyr Phe Thr Lys Lys 
        35                  40                  45              


Gly Ala Thr Lys Lys Ala Ser Leu Ser Thr Gly Gly Arg Tyr Ser Asp 
    50                  55                  60                  


Thr Lys Asn Thr Ala Ser Lys Ser Phe Ser Leu Arg Ile Ser Asp Leu 
65                  70                  75                  80  


Arg Val Glu Asp Ser Gly Thr Tyr His Cys Glu Ala Arg Tyr Ser Trp 
                85                  90                  95      


Tyr Cys Tyr Ser Pro Asp Ser Ser Asn Tyr Ile Glu Gly Gly Gly Thr 
            100                 105                 110         


Ala Leu Thr Val Lys Pro 
        115             


<210>  11
<211>  117
<212>  PRT
<213>  Chiloscyllium plagiosum

<400>  11

Gln Val Gln Leu Gln Glu Ser Gln Arg Val Glu Gln Thr Pro Thr Thr 
1               5                   10                  15      


Thr Thr Lys Glu Ala Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Lys 
            20                  25                  30          


Gly Ser Ser Cys Ala Leu Gly Ser Thr Tyr Trp Tyr Phe Thr Lys Lys 
        35                  40                  45              


Gly Ala Thr Lys Lys Ala Ser Leu Ser Thr Gly Gly Arg Tyr Ser Asp 
    50                  55                  60                  


Thr Lys Asn Thr Ala Ser Lys Ser Phe Ser Leu Arg Ile Ser Asp Leu 
65                  70                  75                  80  


Arg Val Glu Asp Ser Gly Thr Tyr His Cys Glu Ala Tyr Ser Asp Cys 
                85                  90                  95      


Trp Val Glu Asp Gly Gly Ala Pro Tyr Ile Glu Gly Gly Gly Thr Val 
            100                 105                 110         


Val Thr Val Asn Pro 
        115         


<210>  12
<211>  118
<212>  PRT
<213>  Chiloscyllium plagiosum

<400>  12

Gln Val Gln Leu Gln Glu Ser Gln Arg Val Glu Gln Thr Pro Thr Thr 
1               5                   10                  15      


Thr Thr Lys Glu Ala Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Leu 
            20                  25                  30          


Gly Ser Ser Cys Ala Leu Gly Ser Thr Asn Trp Tyr Phe Thr Lys Lys 
        35                  40                  45              


Gly Ala Thr Lys Lys Ala Ser Leu Ser Thr Gly Gly Arg Tyr Ser Asp 
    50                  55                  60                  


Thr Lys Asn Thr Ala Ser Lys Ser Phe Ser Leu Arg Ile Ser Asp Leu 
65                  70                  75                  80  


Arg Val Glu Asp Ser Gly Thr Tyr His Cys Glu Ala Tyr Thr Cys Trp 
                85                  90                  95      


Asp Ser Gly His Thr Gly Gly Tyr Lys Asp Phe Val Gly Gly Gly Thr 
            100                 105                 110         


Thr Leu Thr Val Lys Pro 
        115             


<210>  13
<211>  118
<212>  PRT
<213>  Chiloscyllium plagiosum

<400>  13

Gln Val Gln Leu Gln Glu Ser Pro Arg Val Glu Gln Thr Pro Thr Thr 
1               5                   10                  15      


Thr Thr Lys Lys Ala Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Lys 
            20                  25                  30          


Gly Ser Ser Cys Ala Leu Gly Ser Thr Tyr Trp Tyr Phe Thr Lys Lys 
        35                  40                  45              


Gly Ala Thr Lys Lys Ala Ser Leu Ser Thr Gly Gly Arg Tyr Ser Asp 
    50                  55                  60                  


Thr Lys Asn Thr Ala Ser Lys Ser Phe Ser Leu Arg Ile Ser Asp Leu 
65                  70                  75                  80  


Arg Val Glu Asp Ser Gly Thr Tyr His Cys Glu Ala Arg Tyr Ser Trp 
                85                  90                  95      


Tyr Cys Tyr Ser Pro Asp Ser Ser Asn Tyr Ile Glu Gly Gly Gly Thr 
            100                 105                 110         


Ile Leu Thr Val Asn Pro 
        115             


<210>  14
<211>  118
<212>  PRT
<213>  Chiloscyllium plagiosum

<400>  14

Gln Val Gln Leu Gln Glu Ser Gln Arg Val Glu Gln Thr Pro Thr Thr 
1               5                   10                  15      


Thr Thr Lys Glu Ala Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Leu 
            20                  25                  30          


Gly Ser Ser Cys Ala Leu Gly Ser Thr Asn Trp Tyr Phe Thr Lys Lys 
        35                  40                  45              


Gly Ala Thr Lys Lys Ala Ser Leu Ser Thr Gly Gly Arg Tyr Ser Asp 
    50                  55                  60                  


Thr Lys Asn Thr Ala Ser Lys Ser Phe Ser Leu Arg Ile Ser Asp Leu 
65                  70                  75                  80  


Arg Val Glu Asp Ser Gly Thr Tyr His Cys Glu Ala Tyr Thr Cys Trp 
                85                  90                  95      


Asp Ser Gly His Thr Gly Gly Tyr Lys Asp Phe Val Gly Gly Gly Thr 
            100                 105                 110         


Thr Leu Thr Val Lys Pro 
        115             


