                         SEQUENCE LISTING

<110>  ɶ̼Ƽ޹˾
 
<120>  ׵׵ͻ塢׿׼Ӧ

<130>  PCT21129CN

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  289
<212>  PRT
<213>  Simiduia agarivorans

<400>  1

Met Phe Arg Ser Ala Leu Leu Leu Cys Ala Gly Ala Cys Leu Phe Ile 
1               5                   10                  15      


Ser Gly Cys Gln Thr Ala Glu Lys Thr Ser Ala Ala Leu Phe Gly Pro 
            20                  25                  30          


Gly Val Glu His Ala Ser Leu Thr Pro Arg Ala Ser Thr Tyr Ser Asp 
        35                  40                  45              


Leu Lys Ser Leu Pro Pro Pro Arg Gly Lys Ile Val Ala Thr Val Tyr 
    50                  55                  60                  


Asn Phe Arg Asp Gln Thr Gly Gln Tyr Lys Pro Ile Pro Ala Ser Ser 
65                  70                  75                  80  


Phe Ser Thr Ala Val Thr Gln Gly Ala Thr Ala Met Leu Val Asn Ala 
                85                  90                  95      


Met Asn Glu Ser Gly Trp Phe Ile Pro Leu Glu Arg Glu Gly Leu Gln 
            100                 105                 110         


Asn Leu Leu Thr Glu Arg Lys Ile Ile Arg Ala Ala Leu Lys Lys Pro 
        115                 120                 125             


Gly Thr Pro Thr Asn Asn Asp Asp Asp Leu Pro Ser Leu Leu Ala Ala 
    130                 135                 140                 


Asn Val Leu Ile Glu Gly Gly Ile Val Ala Tyr Glu Ser Asn Ile Lys 
145                 150                 155                 160 


Thr Gly Gly Ala Gly Ala Arg Tyr Phe Gly Ile Gly Ala Ser Glu Gln 
                165                 170                 175     


Tyr Arg Thr Asp Gln Val Thr Val Asn Leu Arg Ala Ile Asp Ile Arg 
            180                 185                 190         


Ser Gly Arg Ile Leu His Ser Val Leu Thr Thr Lys Ser Ile Leu Ser 
        195                 200                 205             


Gln Glu Ile Thr Thr Gly Val Phe Arg Phe Val Glu Tyr Lys Arg Leu 
    210                 215                 220                 


Leu Glu Ile Glu Ala Gly Thr Thr Thr Asn Glu Pro Ala Gln Leu Cys 
225                 230                 235                 240 


Val Leu Ser Ala Ile Glu Ala Ala Leu Ile His Leu Ile Val Asp Gly 
                245                 250                 255     


Ile Glu Lys Asn Ser Trp Thr Leu Met Asn Asn Glu Asp Ile Asn Asn 
            260                 265                 270         


Ala Thr Leu Lys Gln Tyr Met Ser Ala Lys Pro Ala Leu Gln Lys Gln 
        275                 280                 285             


Thr 
    


<210>  2
<211>  870
<212>  DNA
<213>  Simiduia agarivorans

<400>  2
atgttccgat ccgcattgct tttgtgcgca ggcgcctgcc tgtttatttc tggctgccag       60

accgctgaaa aaacctctgc cgccctgttt gggcccggcg ttgaacacgc atcactcaca      120

cccagggcga gtacttacag cgacctgaag tcattgccgc cgcccagggg gaagattgtc      180

gcaacggttt acaatttccg cgaccagacc gggcaataca agccgattcc tgccagctcc      240

ttttccaccg cggtaacgca aggtgccacc gccatgctgg tgaatgccat gaatgaatcc      300

ggttggttca ttccgcttga acgggaaggc ctgcagaatt tgcttaccga acgaaaaatt      360

attcgcgccg cgctgaaaaa gccgggcacc cccacaaata atgacgatga tttgccttct      420

ttgctggccg ccaatgtact gatagagggc ggaattgtgg cctatgagtc caacataaaa      480

accggcggcg ctggcgcccg ctattttggt attggcgcct cagagcagta ccgtaccgac      540

caggtgactg taaacttacg ggctatcgac atccgctccg gcagaatcct gcacagcgtt      600

ctgaccacca agtcaatttt gtcccaagag atcaccaccg gcgtattccg ctttgtggaa      660

tacaaacgac tgctggaaat cgaggccggc accaccacta atgagcctgc acaactgtgc      720

gttttatccg ccatagaagc agcgctaata cacctgattg ttgatggcat cgaaaaaaat      780

tcctggacgc taatgaacaa tgaagacatc aacaatgcaa ctttaaagca atatatgtcg      840

gctaaacctg cgctgcaaaa acaaacgtag                                       870


<210>  3
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  3
tttttttttt                                                              10


<210>  4
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  4
aatgtacttc gttcagttac gtattgct                                          28


<210>  5
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  5
gcaatacgta actgaacgaa gttcactatc gcattctcat ga                          42


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  6
tcatgagaat gcgatagtga                                                   20


<210>  7
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  7
aaaaaaaaaa aaaaaaaaaa aaaaaaaa                                          28


<210>  8
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  8
aaaaaaaaaa aa                                                           12


<210>  9
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  9
aaaaaaaaaa aaaatctctg aatctctgaa tctctgaatc tctaaaaaaa aaaaagaaaa       60

aaaaaaaaca aaaaaaaaaa ataaaaaaaa aaaaagcaat acgtaactga acgaagtaca      120

ttaaaaaaaa aa                                                          132


<210>  10
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  10
atcctttttt ttttaatgta cttcgttcag ttacgtattg ct                          42


<210>  11
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  11
tttttttttt ttattttttt tttttgtttt ttttttttct tttttttttt tagagattca       60

gagattcaga gattcagaga tttttttttt tttt                                   94


<210>  12
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  12
tttttttttt tt                                                           12


<210>  13
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  13
tttttttttt tttttttttt tttttttt                                          28


<210>  14
<211>  441
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  helicase

<400>  14

Met Ile Thr Ile Asp Gln Leu Thr Glu Gly Gln Phe Asp Ser Leu Gln 
1               5                   10                  15      


Arg Ala Lys Val Leu Ile Gln Glu Ala Thr Lys Asn Asp Gly Asn Trp 
            20                  25                  30          


Asn His Arg Thr Lys His Leu Thr Ile Asn Gly Pro Ala Gly Thr Gly 
        35                  40                  45              


Lys Thr Thr Met Met Lys Phe Leu Val Ser Trp Leu Arg Asp Glu Gly 
    50                  55                  60                  


Ile Thr Gly Val Ala Leu Ala Ala Pro Thr His Ala Ala Lys Lys Val 
65                  70                  75                  80  


Leu Ala Asn Ala Val Gly Glu Glu Val Ser Thr Ile His Ser Ile Leu 
                85                  90                  95      


Lys Ile Asn Pro Thr Thr Tyr Glu Cys Lys Gln Phe Phe Glu Gln Ser 
            100                 105                 110         


Ala Pro Pro Asp Leu Ser Lys Ile Arg Ile Leu Ile Cys Glu Glu Cys 
        115                 120                 125             


Ser Phe Tyr Asp Ile Lys Leu Phe Glu Ile Leu Met Asn Ser Ile Gln 
    130                 135                 140                 


Pro Trp Thr Ile Ile Ile Gly Ile Gly Asp Arg Ala Gln Leu Arg Pro 
145                 150                 155                 160 


Ala Asp Asp Lys Gly Ile Ser Arg Phe Phe Thr Asp Gln Arg Phe Glu 
                165                 170                 175     


Gln Thr Tyr Leu Thr Glu Ile Lys Arg Ser Asn Met Pro Ile Ile Glu 
            180                 185                 190         


Val Ala Thr Glu Ile Arg Asn Gly Gly Trp Ile Arg Glu Asn Ile Ile 
        195                 200                 205             


Asp Asp Leu Gly Val Lys Gln Asp Lys Ser Val Ser Glu Phe Met Thr 
    210                 215                 220                 


Asn Tyr Phe Lys Val Val Lys Ser Ile Asp Asp Leu Tyr Glu Thr Arg 
225                 230                 235                 240 


Met Tyr Ala Tyr Thr Asn Asn Ser Val Asp Thr Leu Asn Lys Ile Ile 
                245                 250                 255     


Arg Lys Lys Leu Tyr Glu Thr Glu Gln Asp Phe Ile Val Gly Glu Pro 
            260                 265                 270         


Ile Val Met Gln Glu Pro Leu Ile Arg Asp Ile Asn Tyr Glu Gly Lys 
        275                 280                 285             


Arg Phe Gln Glu Ile Val Phe Asn Asn Gly Glu Tyr Leu Glu Val Ser 
    290                 295                 300                 


Glu Ile Lys Pro Met Glu Ser Val Leu Lys Cys Arg Asn Ile Asp Tyr 
305                 310                 315                 320 


Gln Leu Val Leu His Tyr Tyr Gln Leu Lys Val Lys Ser Ile Asp Thr 
                325                 330                 335     


Gly Glu Ser Gly Leu Ile Asn Thr Ile Ser Asp Lys Asn Glu Leu Asn 
            340                 345                 350         


Lys Phe Tyr Met Phe Leu Gly Lys Val Cys Gln Asp Tyr Lys Ser Gly 
        355                 360                 365             


Thr Ile Lys Ala Phe Trp Asp Asp Phe Trp Lys Ile Lys Asn Asn Tyr 
    370                 375                 380                 


His Arg Val Lys Pro Leu Pro Val Ser Thr Ile His Lys Gly Gln Gly 
385                 390                 395                 400 


Ser Thr Val Asp Asn Ser Phe Leu Tyr Thr Pro Cys Ile Thr Lys Tyr 
                405                 410                 415     


Ala Glu Pro Asp Leu Ala Ser Gln Leu Leu Tyr Val Gly Val Thr Arg 
            420                 425                 430         


Ala Arg His Asn Val Asn Phe Val Gly 
        435                 440     


<210>  15
<211>  1326
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  helicase

<400>  15
atgatcacca tcgaccagct gaccgaaggt cagttcgact ctctgcagcg tgctaaagtt       60

ctgatccagg aagctaccaa aaacgacggt aactggaacc accgtaccaa acacctgacc      120

atcaacggtc cggctggtac cggtaaaacc accatgatga aattcctggt ttcttggctg      180

cgtgacgaag gtatcaccgg tgttgctctg gctgctccga cccacgctgc taaaaaagtt      240

ctggctaacg ctgttggtga agaagtttct accatccact ctatcctgaa aatcaacccg      300

accacctacg aatgcaaaca gttcttcgaa cagtctgctc cgccggacct gtctaaaatc      360

cgtatcctga tctgcgaaga atgctctttc tacgacatca aactgttcga aatcctgatg      420

aactctatcc agccgtggac catcatcatc ggtatcggtg accgtgctca gctgcgtccg      480

gctgacgaca aaggtatctc tcgtttcttc accgaccagc gtttcgaaca gacctacctg      540

accgaaatca aacgttctaa catgccgatc atcgaagttg ctaccgaaat ccgtaacggt      600

ggttggattc gtgaaaacat catcgacgac ctgggtgtta aacaggacaa atctgtttct      660

gaatttatga ccaactactt caaagttgtt aaatctatcg acgacctgta cgaaacccgt      720

atgtacgctt acaccaacaa ctctgttgac accctgaaca aaatcatccg taaaaaactg      780

tacgaaaccg aacaggactt catcgttggt gaaccgatcg ttatgcagga accgctgatc      840

cgtgacatca actacgaagg taaacgtttc caggaaatcg ttttcaacaa cggtgaatac      900

ctggaagttt ctgaaatcaa accgatggaa tctgttctga aatgccgtaa catcgactac      960

cagctggttc tgcactacta ccagctgaaa gttaaatcta tcgacaccgg tgaatctggt     1020

ctgatcaaca ccatctctga caaaaacgaa ctgaacaaat tctacatgtt cctgggtaaa     1080

gtttgccagg actacaaatc tggtaccatc aaagcgttct gggacgactt ctggaaaatc     1140

aaaaacaact accaccgtgt taaaccgctg ccggtttcta ccatccacaa aggtcagggt     1200

tctaccgttg acaactcttt cctgtacacc ccgtgcatca ccaaatacgc tgaaccggac     1260

ctggcttctc agctgctgta cgttggtgtt acccgtgctc gtcacaacgt taacttcgtt     1320

ggttaa                                                                1326


<210>  16
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 1

<400>  16

Met Phe Arg Ser Ala Leu Leu Leu Cys Ala Gly Ala Cys Leu Phe Ile 
1               5                   10                  15      


Ser Gly Cys Gln Thr Ala Glu Lys Thr Ser Ala Ala Leu Phe Gly Pro 
            20                  25                  30          


Gly Val Glu His Ala Ser Leu Thr Pro Arg Ala Ser Thr Tyr Ser Asp 
        35                  40                  45              


Leu Lys Ser Leu Pro Pro Pro Arg Gly Lys Ile Val Ala Thr Val Tyr 
    50                  55                  60                  


Asn Phe Arg Asp Gln Thr Gly Gln Tyr Lys Pro Tyr Pro Ala Val Ala 
65                  70                  75                  80  


Ser Thr Ala Val Thr Gln Gly Ala Thr Ala Met Leu Val Asn Ala Met 
                85                  90                  95      


Asn Glu Ser Gly Trp Phe Ile Pro Leu Glu Arg Glu Gly Leu Gln Asn 
            100                 105                 110         


Leu Leu Thr Glu Arg Lys Ile Ile Arg Ala Ala Leu Lys Lys Pro Gly 
        115                 120                 125             


Thr Pro Thr Asn Asn Asp Asp Asp Leu Pro Ser Leu Leu Ala Ala Asn 
    130                 135                 140                 


Val Leu Ile Glu Gly Gly Ile Val Ala Tyr Glu Ser Asn Ile Lys Thr 
145                 150                 155                 160 


Gly Gly Ala Gly Ala Arg Tyr Phe Gly Ile Gly Ala Ser Glu Gln Tyr 
                165                 170                 175     


Arg Thr Asp Gln Val Thr Val Asn Leu Arg Ala Ile Asp Ile Arg Ser 
            180                 185                 190         


Gly Arg Ile Leu His Ser Val Leu Thr Thr Lys Ser Ile Leu Ser Gln 
        195                 200                 205             


Glu Ile Thr Thr Gly Val Phe Arg Phe Val Glu Tyr Lys Arg Leu Leu 
    210                 215                 220                 


Glu Ile Glu Ala Gly Thr Thr Thr Asn Glu Pro Ala Gln Leu Cys Val 
225                 230                 235                 240 


Leu Ser Ala Ile Glu Ala Ala Leu Ile His Leu Ile Val Asp Gly Ile 
                245                 250                 255     


Glu Lys Asn Ser Trp Thr Leu Met Asn Asn Glu Asp Ile Asn Asn Ala 
            260                 265                 270         


Thr Leu Lys Gln Tyr Met Ser Ala Lys Pro Ala Leu Gln Lys Gln Thr 
        275                 280                 285             


<210>  17
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 2

<400>  17

Met Phe Arg Ser Ala Leu Leu Leu Cys Ala Gly Ala Cys Leu Phe Ile 
1               5                   10                  15      


Ser Gly Cys Gln Thr Ala Glu Lys Thr Ser Ala Ala Leu Phe Gly Pro 
            20                  25                  30          


Gly Val Glu His Ala Ser Leu Thr Pro Arg Ala Ser Thr Tyr Ser Asp 
        35                  40                  45              


Leu Lys Ser Leu Pro Pro Pro Arg Gly Lys Ile Val Ala Thr Val Tyr 
    50                  55                  60                  


Asn Phe Arg Asp Leu Thr Gly Gln Tyr Lys Pro Tyr Pro Ala Asn Ala 
65                  70                  75                  80  


Ser Thr Ala Val Thr Gln Gly Ala Thr Ala Met Leu Val Asn Ala Met 
                85                  90                  95      


Asn Glu Ser Gly Trp Phe Ile Pro Leu Glu Arg Glu Gly Leu Gln Asn 
            100                 105                 110         


Leu Leu Thr Glu Arg Lys Ile Ile Arg Ala Ala Leu Lys Lys Pro Gly 
        115                 120                 125             


Thr Pro Thr Asn Asn Asp Lys Asp Leu Pro Ser Leu Leu Ala Ala Asn 
    130                 135                 140                 


Val Leu Ile Glu Gly Gly Ile Val Ala Tyr Glu Ser Asn Ile Lys Thr 
145                 150                 155                 160 


Gly Gly Ala Gly Ala Arg Tyr Phe Gly Ile Gly Ala Ser Glu Gln Tyr 
                165                 170                 175     


Arg Thr Asp Gln Val Thr Val Asn Leu Arg Ala Ile Asp Ile Arg Ser 
            180                 185                 190         


Gly Arg Ile Leu His Ser Val Leu Thr Thr Lys Ser Ile Leu Ser Gln 
        195                 200                 205             


Glu Ile Thr Thr Gly Val Phe Arg Phe Val Ile Tyr Lys Arg Leu Leu 
    210                 215                 220                 


Glu Ile Glu Ala Gly Thr Thr Thr Asn Glu Pro Ala Gln Leu Cys Val 
225                 230                 235                 240 


Leu Ser Ala Ile Glu Ala Ala Leu Ile His Leu Ile Val Asp Gly Ile 
                245                 250                 255     


Glu Lys Asn Ser Trp Thr Leu Met Asn Asn Glu Asp Ile Asn Asn Ala 
            260                 265                 270         


Thr Leu Lys Gln Tyr Met Ser Ala Lys Pro Ala Leu Gln Lys Gln Thr 
        275                 280                 285             


<210>  18
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 3

<400>  18

Met Phe Arg Ser Ala Leu Leu Leu Cys Ala Gly Ala Cys Leu Phe Ile 
1               5                   10                  15      


Ser Gly Cys Gln Thr Ala Glu Lys Thr Ser Ala Ala Leu Phe Gly Pro 
            20                  25                  30          


Gly Val Glu His Ala Ser Leu Thr Pro Arg Ala Ser Thr Tyr Ser Asp 
        35                  40                  45              


Leu Lys Ser Leu Pro Pro Pro Arg Gly Lys Ile Val Ala Thr Val Tyr 
    50                  55                  60                  


Asn Phe Arg Asp Gln Thr Gly Gln Tyr Lys Pro Tyr Pro Ala Gly Gly 
65                  70                  75                  80  


Ser Thr Ala Val Thr Gln Gly Ala Thr Ala Met Leu Val Asn Ala Met 
                85                  90                  95      


Asn Glu Ser Gly Trp Phe Ile Pro Leu Glu Arg Glu Gly Leu Gln Asn 
            100                 105                 110         


Leu Leu Thr Glu Arg Lys Ile Ile Arg Ala Ala Leu Lys Lys Pro Gly 
        115                 120                 125             


Thr Pro Thr Asn Asn Asp Asp Asp Leu Pro Ser Leu Leu Ala Ala Asn 
    130                 135                 140                 


Val Leu Ile Glu Gly Gly Ile Val Ala Tyr Glu Ser Asn Ile Lys Thr 
145                 150                 155                 160 


Gly Gly Ala Gly Ala Arg Tyr Phe Gly Ile Gly Ala Ser Glu Gln Tyr 
                165                 170                 175     


Arg Thr Asp Gln Val Thr Val Asn Leu Arg Ala Ile Asp Ile Arg Ser 
            180                 185                 190         


Gly Arg Ile Leu His Ser Val Leu Thr Thr Lys Ser Ile Leu Ser Gln 
        195                 200                 205             


Glu Ile Thr Thr Gly Val Phe Arg Phe Val Glu Tyr Lys Arg Leu Leu 
    210                 215                 220                 


Glu Ile Glu Ala Gly Thr Thr Thr Asn Glu Pro Ala Gln Leu Cys Val 
225                 230                 235                 240 


Leu Ser Ala Ile Glu Ala Ala Leu Ile His Leu Ile Val Asp Gly Ile 
                245                 250                 255     


Glu Lys Asn Ser Trp Thr Leu Met Asn Asn Glu Asp Ile Asn Asn Ala 
            260                 265                 270         


Thr Leu Lys Gln Tyr Met Ser Ala Lys Pro Ala Leu Gln Lys Gln Thr 
        275                 280                 285             


<210>  19
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 4

<400>  19

Met Phe Arg Ser Ala Leu Leu Leu Cys Ala Gly Ala Cys Leu Phe Ile 
1               5                   10                  15      


Ser Gly Cys Gln Thr Ala Glu Lys Thr Ser Ala Ala Leu Phe Gly Pro 
            20                  25                  30          


Gly Val Glu His Ala Ser Leu Thr Pro Arg Ala Ser Thr Tyr Ser Asp 
        35                  40                  45              


Leu Lys Ser Leu Pro Pro Pro Arg Gly Lys Ile Val Ala Thr Val Tyr 
    50                  55                  60                  


Asn Phe Arg Asp Gln Thr Gly Gln Tyr Lys Pro Ile Pro Ala Val Ala 
65                  70                  75                  80  


Ser Thr Ala Val Thr Gln Gly Ala Thr Ala Met Leu Val Asn Ala Met 
                85                  90                  95      


Asn Glu Ser Gly Trp Phe Ile Pro Leu Glu Arg Glu Gly Leu Gln Asn 
            100                 105                 110         


Leu Leu Thr Glu Arg Lys Ile Ile Arg Ala Ala Leu Lys Lys Pro Gly 
        115                 120                 125             


Thr Pro Thr Asn Asn Asp Asp Asp Leu Pro Ser Leu Leu Ala Ala Asn 
    130                 135                 140                 


Val Leu Ile Glu Gly Gly Ile Val Ala Tyr Glu Ser Asn Ile Lys Thr 
145                 150                 155                 160 


Gly Gly Ala Gly Ala Arg Tyr Phe Gly Ile Gly Ala Ser Glu Gln Tyr 
                165                 170                 175     


Arg Thr Asp Gln Val Thr Val Asn Leu Arg Ala Ile Asp Ile Arg Ser 
            180                 185                 190         


Gly Arg Ile Leu His Ser Val Leu Thr Thr Lys Ser Ile Leu Ser Gln 
        195                 200                 205             


Glu Ile Thr Thr Gly Val Phe Arg Phe Val Glu Tyr Lys Arg Leu Leu 
    210                 215                 220                 


Glu Ile Glu Ala Gly Thr Thr Thr Asn Glu Pro Ala Gln Leu Cys Val 
225                 230                 235                 240 


Leu Ser Ala Ile Glu Ala Ala Leu Ile His Leu Ile Val Asp Gly Ile 
                245                 250                 255     


Glu Lys Asn Ser Trp Thr Leu Met Asn Asn Glu Asp Ile Asn Asn Ala 
            260                 265                 270         


Thr Leu Lys Gln Tyr Met Ser Ala Lys Pro Ala Leu Gln Lys Gln Thr 
        275                 280                 285             


