﻿                         序列表

<110>  安徽丰原生物技术股份有限公司

<120>  一种生产L-丙氨酸的基因工程菌株及其构建方法和应用

<130>  2021

<160>  32

<170>  SIPOSequenceListing 1.0

<210>  3
<211>  1119
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  3
atgaaaatcg gcatcccgaa agaaatcaaa aacaacgaaa accgcgtcgc gatcacgccg        60
gcgggcgtca tgacgctggt caaagcgggc catgacgtct atgtcgaaac ggaagcgggc       120
gcgggcagcg gctttagcga cagcgaatat gaaaaagcgg gcgcggtcat cgtcccgaac       180
gcggaagacg cgtggacggc ggaaatggtc ctgaaagtca aagaaccgct ggcggaagaa       240
tttcgctatt ttcgcccggg cctgatcctg tttacgtatc tgcatctggc ggcggcggaa       300
gcgctgacga aagcgctggt cgaacagaaa gtcgtcggca tcgcgtatga aacggtccag       360
ctggcgaacg gcagcctgcc gctgctgacg ccgatgagcg aagtcgcggg ccgcatgagc       420
gtccaggtcg gcgcgcagtt tctggaaaaa ccgcatggcg gcaaaggcat cctgctgggc       480
ggcgtcccgg gcgtccgccg cggcaaagtc acgatcatcg gcggcggcac ggcgggcacg       540
aacgcggcga aaatcgcggt cggcctgggc agcgacgtca cgatcctgga catcaacgcg       600
gaacgcctgc gcgaactgga cgacctgttt ggcgaccatg tcacgacgct gatgagcaac       660
agctatcata tcgcggaatg cgtccgcgaa agcgacctgg tcgtcggcgc ggtcctgatc       720
ccgggcgcga aagcgccgaa actggtcacg gaagaaatgg tccgcagcat gacgccgggc       780
agcgtcctgg tcgacatcgc gatcgaccag ggcggcatct ttgaaacgac ggaccgcgtc       840
acgacgcatg acgacccgac gtatgtcaaa catggcgtcg tccattatgc ggtcgcgaac       900
atgccgggcg cggtcccgcg cacgagcacg tttgcgctga cgaacgtcac gatcccgtat       960
gcgctgcaga tcgcgaacaa aggctatcgc gcggcgtgcc tggacaaccc ggcgctgctg      1020
aaaggcatca acacgctgga cggccatatc gtctatgaag cggtcgcggc ggcgcataac      1080
atgccgtata cggacgtcca tagcctgctg catggctaa                             1119

<210>  2
<211>  300
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  2
aaggtgacgc ctatttcact ttctagctgt ttaatctgct ggctgagcgg aggctgagtc        60
atgttcagcc gaagagctgc ttttccgaaa tgcagttctt cggcaacaac cataaaataa       120
cgaagatggc gcagctccat taatcactca ttcctttctg aatgcgattt cagtcgtttt       180
acatattaat tgtaagacaa agaagtattg gaaaacaatt tccacaagat gtatatttaa       240
taatacaata attttattaa aaattcattg taaatgaatg aaaatggagg agtgagggct       300

<210>  3
<211>  48
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  3
cctcgcgtcg ggcgatatcg gatccgaagg ggaaagtctt cgatttct                     48

<210>  4
<211>  37
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  4
agagggcttt ttcatgctga agaggtcaaa aagagcc                                 37

<210>  5
<211>  38
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  5
tttgacctct tcagcatgaa aaagccctct ttgaaaag                                38

<210>  6
<211>  47
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  6
ccatggtacc cgggagctcg aattccataa gaccgctgat gacaagc                      47

<210>  7
<211>  47
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  7
tccagcctcg cgtcgggcga tatcgtcccc ataacaacgg aatcatc                      47

<210>  8
<211>  37
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  8
aataggcgtc accttgactc atcattcctt tgccgtt                                 37

<210>  9
<211>  38
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  9
aaggaatgat gagtcaaggt gacgcctatt tcactttc                                38

<210>  10
<211>  36
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  10
tccaattcgc ttcatagccc tcactcctcc attttc                                  36

<210>  11
<211>  39
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  11
ggaggagtga gggctatgaa gcgaattgga gtattgaca                               39

<210>  12
<211>  36
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  12
cttcatggtg ttcagttaca atacagtcgc atggcc                                  36

<210>  13
<211>  42
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  13
gcgactgtat tgtaactgaa caccatgaag atactaacat ca                           42

<210>  14
<211>  48
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  14
actagacaga tctatcgatg catgctttcc cttattcctt taaacccg                     48

<210>  15
<211>  37
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  15
aataggcgtc accttgctga agaggtcaaa aagagcc                                 37

<210>  16
<211>  39
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  16
tttgacctct tcagcaaggt gacgcctatt tcactttct                               39

<210>  17
<211>  36
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  17
agagggcttt ttcatttagc catgcagcag gctatg                                  36

<210>  18
<211>  38
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  18
ctgctgcatg gctaaatgaa aaagccctct ttgaaaag                                38

<210>  19
<211>  50
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  19
cctcgcgtcg ggcgatatcg gatccaaaat atgacgctgt ctcaaattga                   50

<210>  20
<211>  40
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  20
ctaattcatc aatttgacac ttcctgttcc ttgtttcact                              40

<210>  21
<211>  39
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  21
ggaacaggaa gtgtcaaatt gatgaattag cggaaaaac                               39

<210>  22
<211>  48
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  22
ccatggtacc cgggagctcg aattccggag tctctttcaa aaccgtag                     48

<210>  23
<211>  47
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  23
cctcgcgtcg ggcgatatcg gatccaaaat catgtaagcc cattccg                      47

<210>  24
<211>  37
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  24
gtgagtatgg gaaaacaacg ctcccttctt tcttgtc                                 37

<210>  25
<211>  36
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  25
aagaagggag cgttgttttc ccatactcac aggccg                                  36

<210>  26
<211>  47
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  26
ccatggtacc cgggagctcg aattctaaaa tgaaggtggt ccgggat                      47

<210>  27
<211>  963
<212>  DNA
<213>  凝结芽孢杆菌(Bacillus coagulans)

<400>  27
atgaagcgaa ttggagtatt gacaagcggc ggcgatgcac cggggatgaa tgcggcggtc        60
cgcgcgattg cccgtaaagg gatttatcac ggcctggaag tttacggcat tcgccaaggt       120
tataacggat tgattcaagg aaacatccaa aagctcgaag caggatctgt tggcgatatt       180
ctccagcggg gcggcacggt tttgcagtcg gcaagaagcg aagaattcaa aacgccggaa       240
gggcagcaaa aagcgatcag gcagctgaaa gaccatggca ttgaagcgct cgttgtgatc       300
ggcggcgacg gttcctacca aggggccaaa aagttgacgg aacagggctt taactgcatt       360
ggtgtgccag ggacaatcga taacgacatc ccggggacgg attttacaat cggttttgat       420
acggcattga acacagtgct tgatgcgatt gataaaattc gcgacaccgc ttcttcccac       480
gaacgcacct ttattattga agtcatgggc agaaatgccg gggatatcgc gctctggtcc       540
ggcctggccg gcggagccga atcgattatt attccggaag aaaaatatga cttaaaagat       600
gtcgtggagc gtcttgaaca ggggagaaaa cgcggcaaac gccacagcat catcattgtc       660
gcggaaggcg tgatgagcgg caacgagttt gctgaacaat tgaaaaaaac cggtgtgatc       720
ggcgataccc gcgtttctgt tctcggccat atccagcgcg gcggttctcc gacggcattt       780
gaccgcgtgc ttgcaagccg cctcggcgca agggctgttg aactgctgct tgaaggaaaa       840
gggggccgcg ctgtcggcat tcaaaataac cagctggttg accacgatat ccttgagatt       900
ctcggaaaac cgcacgccgt taataaaaac atgtacaagc tgtcgaaaga attgtcgatc       960
taa                                                                     963

<210>  28
<211>  1761
<212>  DNA
<213>  凝结芽孢杆菌(Bacillus coagulans)

<400>  28
atgaaaaaaa ccaaaattgt atgtacaatc ggacctgcca gtgaaagtgt ggaaatgctt        60
gaaagattaa tggcaaacgg gatggatgtt tgccgcctga acttctcgca cggcagccat       120
gaggaacatc ttgcccggat taaaaatatc cgtgaagctg caaaaaacca aaacaaaacg       180
atcgggcttc tgctcgatac aaagggcccg gaaatccgca cccatgatat gaaagacggc       240
ggattcgagc tcgttgaagg catgacaccg gtcatttcaa tgacagaagt gctcgggaca       300
ccggaaaaat tttcggtcac atatgaaggg ctgattgatg atgtgcacgt tggctctaaa       360
attttacttg atgacggttt gattgaactg gaagtgacgg ccatcgataa aaacgccggt       420
gaaatccata caaaagtgct gaaccgcggc gttttgaaaa acaaaaaagg tgttaacgtc       480
ccgggtgttt ccgtgaacct tccgggcatc accgaaaaag acgtgagcga tatcctgttc       540
gggcttgaac aaggcattga cttcattgcg gcttcgtttg tacgccggcc gtccgacgtt       600
ttggaaatcc gccagctcct tgaagaacac gatgctttgc atgtgaaaat tttccctaaa       660
attgaaaacc aggaaggcgt cgacaatatc gatgaaatcc ttgcggtatc agacggctta       720
atggttgccc gcggcgacct cggcgttgaa attccgaccg aagcggtgcc gctcgtacaa       780
aaagaaatga tcagaaaatg taatacgctc ggcaaaccgg tgattaccgc aacgcaaatg       840
cttgattcga tgcaacgcaa cccgcgcccg acccgcgcgg aagcaagcga cgtggccaac       900
gccatttttg acggcacgga tgcgatcatg ctttccggcg aaacggcagc cgggaaatat       960
cctgctgaag cggttaagac gatgtacaat attgcggttc atgtggaaaa agcaattaac      1020
catcgcgata ttctgaacaa gcgcagcaag agcacggacc ataatatgac agacgctatc      1080
tgccagtccg ttgcccatac ggctttaaat cttgatgtga atgccattat tgcgccgact      1140
gaaagcggct atacggcacg catgatctcc aaataccgcc cggcggcccc aatcattgct      1200
gtcacgagcg atccgaaagt acaacgcggc ttaactgttg tgtccggcgt atacccacaa      1260
ttgggcacaa aggcaaacaa tacggatgaa atgcttgaaa ttgcagtgga ggaagcgttg      1320
aaatccgaaa tcgtccatca cggcgacctt gtgatcatta cagcaggcgt cccggttggt      1380
gggaaaggca ccaccaacct gatgaaagtg cacctgatcg gtgatatatt ggcaaaaggc      1440
cagggaatcg gcagaaaatc ggcattcggc ccggtcatcg ttgctgaaag ccctgaagaa      1500
gcaaacgcaa aggcaacaga aggttgtgtg ctcgtcacga gaacgaccga caaagaaatc      1560
atgccggcca ttgaaaaatg cgccgcgctg attacggaag aaggcggctt gacaagccat      1620
gctgcagttg ttggcatcaa tgtcggcatt ccggtcattg ttggcgttga aaaagccgtt      1680
tccatttttg aagacgggca ggaagttacg gtagatgcgg caaccggctc ggtttacaac      1740
ggccatgcga ctgtattgta a                                                1761

<210>  29
<211>  1173
<212>  DNA
<213>  地衣芽孢杆菌(Bacillus licheniformis)

<400>  29
atgatgagct taaaaccatt ctatagaaag acatgggccg aaatcgattt aacggcttta        60
aaagaaaacg tccgcaatat gaagcggcac atcggcgagc atgtccgcct gatggccgtc       120
gttaaagcga atgcctacgg acacggggat gcacaggtag cgaaggcggc tcttgcagaa       180
ggggcgtcca ttcttgctgt ggctttattg gatgaagcgc tttcgctgag ggcgcagggg       240
attgaagaac cgattcttgt cctcggtgca gtgccgaccg aatatgcaag cattgccgcg       300
gaaaagcgca ttatcgtgac tggctactcc gtcggctggc tgaaagacgt gctcggtttt       360
ctgaatgagg ccgaagctcc tcttgaatat catttgaaga tcgacacggg catgggccgc       420
cttggctgca aaacggaaga agagatcaaa gaaatgatgg agatgaccga atcgaacgat       480
aagctcaatt gtacgggcgt gttcactcat ttcgccacgg cggacgaaaa ggacaccgat       540
tatttcaaca tgcagcttga ccgctttaaa gagctgatca gccccctccc gcttgaccgt       600
ttgatggtgc attcgtcaaa cagcgccgcg ggtctgcgct tcagggaaca gctatttaat       660
gccgtccgct tcggcatcgg catgtacggt ttggcgccgt caaccgaaat aaaagacgag       720
ctgccgtttc gtctgcggga agtgttttcg cttcataccg aactcaccca tgtgaaaaaa       780
attaaaaaag gcgagagcgt cagctacggg gcgacatata cagctcagcg cgacgaatgg       840
atcgggacag tccccgtcgg gtatgccgac ggatggctga ggcgcctggc cggaacggaa       900
gtgctgatcg acggaaaacg ccaaaaaata gcagggagaa tctgcatgga ccagttcatg       960
atttcccttg ccgaagaata ccctgtcggc acaaaggtta ccttgatcgg aaagcaaaaa      1020
gacgaatgga tctcagtcga cgaaatcgcc caaaatttgc agacgatcaa ttatgaaatt      1080
acctgtatga taagttcaag ggtgccccgt atgtttttgg aaaatgggag tataatggaa      1140
ataaggaatc cgatcttgcc tgatcaatcc tga                                   1173

<210>  30
<211>  1182
<212>  DNA
<213>  地衣芽孢杆菌(Bacillus licheniformis)

<400>  30
atgaaaaagc tttgccgtga agtttgggta gaggtaaatc ttgatgcgat caaaaaaaat        60
ttgcgcgcgt ttcggcggca tattccgaaa aagagcaaaa ttatggctgt cgtaaaagcg       120
aatgcttatg gtcacggatc ggtggaagtt gcacgccatg cacttgaaca tggtgcgagt       180
gagctcgccg ttgcctcggt ggaggaagcg gtcgttttac gaaaagcggg gattaaagcg       240
ccgatccttg tgcttggttt caccccgctg agctgtgtga aagaagcggc agcttggaat       300
atatcgttat cagcttttca agttgactgg attaaagaag cgaacgagat attggaaaat       360
gaagcagatc ctaaccggct ggctgttcat atcaatgtgg ataccggcat ggggcgttta       420
ggtgtacgaa caaaggaaaa gcttttagca atcgtggaag cgctgacggc aagtgaaaac       480
ctcgaatggg aaggaatttt tacgcatttt tccacagctg acgaaccgga tactgagcta       540
accatgattc aacacgaaaa gtttatcagc tttcttcgct ttctgaaaga acaaggcttt       600
aagctgccta cggtgcatat gaacaatacg gccgcggcga tcgcttttcc ggaattcagc       660
gctgatatga ttcgcttagg catcggaatg tatggattat atccttccga ttatatcagg       720
cagcttaatc tcgttaagct tgtgcctgca ctaagcttga aggcgcgaat cgcttatgtg       780
aaaaccatgt tgactgaacc gcggacggtt agttatggtg ctacatatgt tgcagagcgc       840
ggggaagtca ttgccacaat tccggtcggc tatgctgacg gctattcccg tgaactttcc       900
aaccgcggtt ttattcttca tcgaggaaga cgagtgccgg tggcgggaag agtaacaatg       960
gatatgataa tggtcagtct gggagagggt gaaggtaaac aaggagagga agtcgtgatt      1020
tacggccggc aaaagggagc agagatatct gttgatgaaa ttgcggaaat gcttgatacg      1080
atcaactatg aagtggtatc taccataagc tggcgcgtcc ctcgttttta tataagagac      1140
ggcgagattt ttaaaaagtc gaccccgctg ttatacgtgt ag                         1182

<210>  31
<211>  978
<212>  DNA
<213>  地衣芽孢杆菌(Bacillus licheniformis)

<400>  31
atgaaagtaa tttttttttc tatgcacccg tatgaagagg aatttctggg tccgattctg        60
ccgtctgact gggacgtaga aatgaccccg gactttctgg acgaaaccac cgtggaaaag       120
gctaaaggtg cccaggtagt aagcctgttt gtttctgaca aagctgatgg tccggtactg       180
gaagcgctgc attcttacgg tgtgggcctg ctggccctgc gttctgctgg ctatgatcac       240
atcgatattg agaccgcaaa acgcctgggt atcaaagtag ttaacgtgcc agcctattct       300
ccgcacgcta tcgctgacca tactctggct atcatgctgg ctctgattcg tcgtctgcac       360
cgtgcccatg ataaagtgcg cctgggtgat tttgatctgg atggtctgat gggctttgat       420
ctgaacggca aagttgctgg tgtaattggt ctgggtaaaa tcggtcgcct ggtagctacc       480
cgcctgaaag cgtttggttg caaagttctg ggctatgatc catacattca gccggaaatc       540
gtagaaaacg ttgatctgga taccctgatc actcaggctg atatcatttc tattcattgt       600
ccgctgaccc gtgaaaactt tcatatgttt aacgaagaga cttttaagcg tatgaaaccg       660
ggtgctattc tggttaacac cgcgcgtggt ggtctgatcg ataccaaggc cctgctggag       720
gccctgaagt ctggtaaact gggcggcgca gccctggatg tgtatgaata tgaacgtggc       780
ctgtttttta aaaaccacca aaaagaaggt atcaaagacc cgtatctggc ccagctgctg       840
ggtctggcca acgtagtgct gaccggtcat caggcctttc tgacccgtga ggctgtaaaa       900
aacatcgaag aaactaccgt agaaaacatt ctggaatggc aaaagaaccc gcaggcaaag       960
ctgaaaaacg aaatctaa                                                     978

<210>  32
<211>  372
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  32
Met Lys Ile Gly Ile Pro Lys Glu Ile Lys Asn Asn Glu Asn Arg Val 
1               5                   10                  15      
Ala Ile Thr Pro Ala Gly Val Met Thr Leu Val Lys Ala Gly His Asp 
            20                  25                  30          
Val Tyr Val Glu Thr Glu Ala Gly Ala Gly Ser Gly Phe Ser Asp Ser 
        35                  40                  45              
Glu Tyr Glu Lys Ala Gly Ala Val Ile Val Pro Asn Ala Glu Asp Ala 
    50                  55                  60                  
Trp Thr Ala Glu Met Val Leu Lys Val Lys Glu Pro Leu Ala Glu Glu 
65                  70                  75                  80  
Phe Arg Tyr Phe Arg Pro Gly Leu Ile Leu Phe Thr Tyr Leu His Leu 
                85                  90                  95      
Ala Ala Ala Glu Ala Leu Thr Lys Ala Leu Val Glu Gln Lys Val Val 
            100                 105                 110         
Gly Ile Ala Tyr Glu Thr Val Gln Leu Ala Asn Gly Ser Leu Pro Leu 
        115                 120                 125             
Leu Thr Pro Met Ser Glu Val Ala Gly Arg Met Ser Val Gln Val Gly 
    130                 135                 140                 
Ala Gln Phe Leu Glu Lys Pro His Gly Gly Lys Gly Ile Leu Leu Gly 
145                 150                 155                 160 
Gly Val Pro Gly Val Arg Arg Gly Lys Val Thr Ile Ile Gly Gly Gly 
                165                 170                 175     
Thr Ala Gly Thr Asn Ala Ala Lys Ile Ala Val Gly Leu Gly Ser Asp 
            180                 185                 190         
Val Thr Ile Leu Asp Ile Asn Ala Glu Arg Leu Arg Glu Leu Asp Asp 
        195                 200                 205             
Leu Phe Gly Asp His Val Thr Thr Leu Met Ser Asn Ser Tyr His Ile 
    210                 215                 220                 
Ala Glu Cys Val Arg Glu Ser Asp Leu Val Val Gly Ala Val Leu Ile 
225                 230                 235                 240 
Pro Gly Ala Lys Ala Pro Lys Leu Val Thr Glu Glu Met Val Arg Ser 
                245                 250                 255     
Met Thr Pro Gly Ser Val Leu Val Asp Ile Ala Ile Asp Gln Gly Gly 
            260                 265                 270         
Ile Phe Glu Thr Thr Asp Arg Val Thr Thr His Asp Asp Pro Thr Tyr 
        275                 280                 285             
Val Lys His Gly Val Val His Tyr Ala Val Ala Asn Met Pro Gly Ala 
    290                 295                 300                 
Val Pro Arg Thr Ser Thr Phe Ala Leu Thr Asn Val Thr Ile Pro Tyr 
305                 310                 315                 320 
Ala Leu Gln Ile Ala Asn Lys Gly Tyr Arg Ala Ala Cys Leu Asp Asn 
                325                 330                 335     
Pro Ala Leu Leu Lys Gly Ile Asn Thr Leu Asp Gly His Ile Val Tyr 
            340                 345                 350         
Glu Ala Val Ala Ala Ala His Asn Met Pro Tyr Thr Asp Val His Ser 
        355                 360                 365             
Leu Leu His Gly 
    370         

