                         SEQUENCE LISTING

<110>  BioHarmony Therapeutics Inc.
 
<120>  Endolysin polypeptide compositions and methods of use

<130>  101457-5

<150>  US 63/250,140
<151>  2021-09-29

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  218
<212>  PRT
<213>  Helicobacter pylori

<400>  1

Met Ile Leu Val Ala Ser Phe Leu Ile Val Asp Leu Glu Gly Phe Ser 
1               5                   10                  15      


Pro Ser Ile Tyr Thr Asp Lys Thr Gly Asn Pro Thr Ile Gly Tyr Gly 
            20                  25                  30          


Tyr Asn Leu Ser Val Tyr Ser Tyr Glu Gly Lys Arg Ile Thr Lys Ala 
        35                  40                  45              


Tyr Gly Leu Leu Thr Asp Ile Leu Lys Glu Asn Tyr Lys Ala Ile Leu 
    50                  55                  60                  


Ser Tyr Gly Trp Tyr Lys Asn Leu Asp Ala Met Arg Arg Met Val Ile 
65                  70                  75                  80  


Leu Asp Leu Ser Tyr Asn Leu Gly Leu Asn Gly Leu Leu Lys Phe Lys 
                85                  90                  95      


Gln Phe Ile Lys Ala Ile Glu Asp Lys Asn Tyr Ala Leu Ala Val Glu 
            100                 105                 110         


Arg Leu Gln Lys Ser Pro Tyr Phe Asn Gln Val Lys Lys Arg Ala Ser 
        115                 120                 125             


Arg Asn Met Glu Ile Leu Lys Leu Gly Gly Cys Glu Lys His Cys Lys 
    130                 135                 140                 


Lys Lys Tyr Thr Ile Glu Lys Val Ile Lys Glu Val Gly Leu Glu Leu 
145                 150                 155                 160 


Lys Ser Lys Ser Val Met Pro Tyr Phe Phe Asn Glu Tyr Asp Leu Thr 
                165                 170                 175     


Lys Asn Ala Asn Arg Glu Glu Pro Glu Arg Leu Arg Ser Gln Ile Ile 
            180                 185                 190         


Ser Ile Leu Met Lys Thr Pro Lys Gly Arg Glu Ile Leu Lys Glu Tyr 
        195                 200                 205             


Leu Asn Glu Gly Cys Ile Leu Leu Gly Glu 
    210                 215             


<210>  2
<211>  87
<212>  PRT
<213>  Helicobacter pylori

<400>  2

Met Asp Ala Met Arg Arg Met Val Ile Leu Asp Leu Ser Tyr Asn Leu 
1               5                   10                  15      


Gly Leu Ser Gly Leu Leu Lys Phe Lys Gln Phe Ile Lys Ala Ile Glu 
            20                  25                  30          


Asp Lys Asn Tyr Ala Leu Ala Val Glu Arg Leu Gln Lys Ser Pro Tyr 
        35                  40                  45              


Phe Asn Gln Val Arg Lys Arg Ala Ser Arg Asn Met Glu Val Leu Lys 
    50                  55                  60                  


Leu Gly Gly Cys Glu Lys His Cys Lys Lys Lys Tyr Thr Ile Lys Lys 
65                  70                  75                  80  


Val Ile Lys Glu Gly Gly Ala 
                85          


<210>  3
<211>  112
<212>  PRT
<213>  Helicobacter pylori

<400>  3

Met Leu Cys Leu Ser Lys Leu Ile Leu Ala Ala Ser Phe Leu Ile Val 
1               5                   10                  15      


Asp Ser Glu Gly Phe Ser Pro Ser Val Tyr Thr Asp Lys Thr Gly His 
            20                  25                  30          


Pro Thr Ile Gly Tyr Gly Tyr Asn Leu Ser Val Tyr Ser Tyr Glu Ser 
        35                  40                  45              


Lys Arg Ile Thr Lys Pro Gln Ala Tyr Gly Leu Leu Thr Asp Ile Leu 
    50                  55                  60                  


Lys Glu Asn His Lys Ala Leu Leu Ser Tyr Gly Trp Tyr Lys Asn Leu 
65                  70                  75                  80  


Asp Ala Met Arg Arg Met Val Ile Leu Asp Leu Ser Tyr Asn Leu Gly 
                85                  90                  95      


Leu Ser Gly Leu Leu Lys Phe Lys Gln Phe Ile Lys Gly Tyr Arg Gly 
            100                 105                 110         


<210>  4
<211>  147
<212>  PRT
<213>  Helicobacter phage

<400>  4

Met Thr Asn Leu Glu Asn Ala Leu Asn Asn Gly Asn Phe Lys Glu Gln 
1               5                   10                  15      


Val Tyr Ser Ser Leu Glu Gly Val Tyr Gln Ile Ser Lys Val Leu Asn 
            20                  25                  30          


Gln Leu Asp Leu Leu Lys Asn Phe Ser Asp His Asp Leu Glu Ile Asn 
        35                  40                  45              


Ser Phe Tyr Gln Asn Lys Thr Ala Asn Leu Arg Asp Gly Asn Lys Ile 
    50                  55                  60                  


Val Ser Thr Tyr Arg Thr Thr Cys Ile Phe Glu Thr Pro Ser Glu Gln 
65                  70                  75                  80  


Ala Asp Tyr Lys Ile Ala Val Phe Ala Arg Lys His Lys Asp Leu Trp 
                85                  90                  95      


Val Asn Met Asn Tyr Thr Ala Asn Thr Ser Gly Phe Glu Thr Ser Phe 
            100                 105                 110         


Leu Asn Asn Ala Asn Phe Arg Gly Leu Thr Thr Gln Ser Ile Pro Thr 
        115                 120                 125             


Thr Tyr Thr Thr Thr Gly Tyr Phe Thr Ser Ile Pro Lys Arg Ser Phe 
    130                 135                 140                 


Met Lys Leu 
145         


<210>  5
<211>  70
<212>  PRT
<213>  Unknown

<220>
<223>  endoendolysin YP_007005686

<400>  5

Met Asp Leu Thr Asn Leu Glu Asp Ala Leu Asn Asn Gly Asn Phe Lys 
1               5                   10                  15      


Glu Gln Val Tyr Ser Gly Leu Asp Gly Val Tyr Arg Ile Ser Lys Val 
            20                  25                  30          


Leu Asn Gln Leu Asp Leu Leu Lys Asn Phe Ser Glu His Asp Leu Glu 
        35                  40                  45              


Ile Val Gly Gly Asn Gly Trp Val Phe His Glu His Ser Gln Ala Ile 
    50                  55                  60                  


Val Tyr Glu Ile Leu Lys 
65                  70  


<210>  6
<211>  133
<212>  PRT
<213>  Helicobacter pylori

<400>  6

Met Ile Leu Ala Ala Ser Phe Leu Ile Val Asp Ser Glu Gly Phe Ser 
1               5                   10                  15      


Pro Ser Ile Tyr Thr Asp Lys Thr Gly His Pro Thr Ile Gly Tyr Gly 
            20                  25                  30          


Tyr Asn Leu Ser Val Tyr Ser Tyr Glu Gly Lys Arg Ile Thr Lys Ala 
        35                  40                  45              


Tyr Gly Leu Leu Thr Asp Ile Leu Lys Glu Asn Tyr Lys Ala Leu Leu 
    50                  55                  60                  


Ser Tyr Gly Trp Tyr Lys Asn Leu Asp Ala Met Arg Arg Met Val Ile 
65                  70                  75                  80  


Leu Asp Leu Ser Tyr Asn Leu Gly Leu Ser Gly Leu Leu Lys Phe Lys 
                85                  90                  95      


Gln Phe Ile Lys Ala Ile Glu Asp Lys Asn Tyr Ala Leu Ala Val Glu 
            100                 105                 110         


Arg Leu Gln Lys Ser Pro Tyr Phe Asn Gln Val Lys Lys Glu Arg Gln 
        115                 120                 125             


Gly Ile Trp Lys Phe 
    130             


<210>  7
<211>  116
<212>  PRT
<213>  Helicobacter pylori

<400>  7

Met Asp Ser Glu Gly Phe Ser Pro Ser Ile Tyr Thr Asp Lys Thr Gly 
1               5                   10                  15      


His Pro Thr Ile Gly Tyr Gly Tyr Asn Leu Ser Val Tyr Ser Tyr Glu 
            20                  25                  30          


Gly Lys Arg Ile Thr Lys Thr Tyr Gly Leu Leu Thr Asp Ile Leu Ser 
        35                  40                  45              


Tyr Gly Trp Tyr Lys Asn Leu Asp Ala Met Arg Arg Met Val Ile Leu 
    50                  55                  60                  


Asp Leu Ser Tyr Asn Leu Gly Leu Asn Gly Leu Leu Lys Phe Lys Gln 
65                  70                  75                  80  


Phe Ile Lys Ala Ile Glu Asp Lys Asn Tyr Ala Leu Ala Val Glu Arg 
                85                  90                  95      


Leu Gln Lys Ser Pro Tyr Phe Asn Gln Val Lys Lys Glu Arg Gln Gly 
            100                 105                 110         


Ile Trp Lys Phe 
        115     


<210>  8
<211>  218
<212>  PRT
<213>  Helicobacter pylori

<400>  8

Met Ile Leu Ala Ala Ser Phe Leu Ile Val Asp Ser Glu Gly Phe Ser 
1               5                   10                  15      


Pro Ser Ile Tyr Thr Asp Lys Thr Glu His Pro Thr Ile Gly Tyr Gly 
            20                  25                  30          


Tyr Asn Leu Ser Val Tyr Ser Tyr Glu Gly Lys Arg Ile Thr Lys Ala 
        35                  40                  45              


Tyr Gly Leu Leu Thr Asp Ile Leu Lys Glu Asn Tyr Lys Ala Leu Leu 
    50                  55                  60                  


Ser Tyr Gly Trp Tyr Lys Asn Leu Asp Ala Met Arg Arg Met Val Ile 
65                  70                  75                  80  


Leu Asp Leu Ser Tyr Asn Leu Gly Leu Asn Gly Leu Leu Lys Phe Lys 
                85                  90                  95      


Gln Phe Ile Lys Ala Ile Glu Asp Lys Asn Tyr Ala Leu Ala Val Glu 
            100                 105                 110         


Arg Leu Gln Lys Ser Pro Tyr Phe Asn Gln Val Lys Lys Arg Ala Ser 
        115                 120                 125             


Arg Asn Met Glu Ile Leu Lys Leu Gly Gly Cys Glu Lys His Cys Lys 
    130                 135                 140                 


Lys Lys Tyr Thr Ile Glu Lys Val Ile Lys Glu Val Gly Leu Glu Leu 
145                 150                 155                 160 


Lys Ser Lys Ser Val Met Pro Tyr Phe Phe Asn Glu Tyr Asp Leu Thr 
                165                 170                 175     


Lys Asn Thr Asn Arg Glu Glu Leu Glu Arg Leu Arg Ser Gln Ile Ile 
            180                 185                 190         


Ser Ile Leu Met Lys Thr Pro Lys Gly Arg Glu Ile Leu Lys Glu Tyr 
        195                 200                 205             


Leu Asn Glu Gly Cys Ile Leu Leu Gly Glu 
    210                 215             


<210>  9
<211>  218
<212>  PRT
<213>  Helicobacter pylori

<400>  9

Met Ile Leu Ala Ala Ser Phe Leu Ile Val Asp Ser Glu Gly Phe Ser 
1               5                   10                  15      


Pro Ser Ile Tyr Thr Asp Lys Thr Glu His Pro Thr Ile Gly Tyr Gly 
            20                  25                  30          


Tyr Asn Leu Ser Val Tyr Ser Tyr Glu Ser Lys Arg Ile Thr Lys Ala 
        35                  40                  45              


Tyr Gly Leu Leu Thr Asp Ile Leu Lys Glu Asn Tyr Lys Ala Leu Leu 
    50                  55                  60                  


Ser Tyr Gly Trp Tyr Lys Asn Leu Asp Ala Met Arg Arg Met Val Ile 
65                  70                  75                  80  


Leu Asp Leu Ser Tyr Asn Leu Gly Leu Asn Gly Leu Leu Lys Phe Lys 
                85                  90                  95      


Gln Phe Ile Lys Ala Ile Glu Asp Lys Asn Tyr Ala Leu Ala Val Glu 
            100                 105                 110         


Arg Leu Gln Lys Ser Pro Tyr Phe Asn Gln Val Lys Lys Arg Ala Ser 
        115                 120                 125             


Arg Asn Met Glu Ile Leu Lys Leu Gly Gly Cys Glu Lys His Cys Lys 
    130                 135                 140                 


Lys Lys Tyr Thr Ile Glu Lys Val Ile Lys Glu Val Gly Leu Glu Leu 
145                 150                 155                 160 


Lys Ser Lys Ser Val Met Pro Tyr Phe Phe Asn Glu Tyr Asp Leu Thr 
                165                 170                 175     


Lys Asn Ala Asn Arg Glu Glu Pro Glu Arg Leu Arg Ser Gln Ile Ile 
            180                 185                 190         


Ser Ile Leu Met Lys Thr Pro Lys Gly Arg Glu Ile Leu Lys Glu Tyr 
        195                 200                 205             


Leu Asn Glu Gly Cys Ile Leu Leu Gly Glu 
    210                 215             


<210>  10
<211>  192
<212>  PRT
<213>  Helicobacter pylori

<400>  10

Met Leu Cys Leu Ser Lys Leu Ile Leu Ala Val Ser Phe Leu Ile Val 
1               5                   10                  15      


Asp Ser Glu Gly Phe Ser Pro Ser Ile Tyr Thr Asp Lys Thr Gly His 
            20                  25                  30          


Pro Thr Ile Gly Tyr Gly Tyr Asn Leu Ser Val Tyr Ser Tyr Glu Ser 
        35                  40                  45              


Lys Arg Ile Thr Lys Pro Gln Ala Tyr Gly Leu Leu Thr Asp Ile Leu 
    50                  55                  60                  


Lys Glu Asn His Lys Ala Leu Leu Ser Tyr Gly Trp Tyr Lys Asn Leu 
65                  70                  75                  80  


Asp Ala Met Arg Arg Met Val Ile Leu Asp Leu Ser Tyr Asn Leu Gly 
                85                  90                  95      


Leu Asn Gly Leu Leu Lys Phe Lys Gln Phe Ile Lys Ala Ile Glu Asp 
            100                 105                 110         


Lys Asn Tyr Ala Leu Ala Val Glu Arg Leu Gln Lys Ser Pro Tyr Phe 
        115                 120                 125             


Asn Gln Val Arg Lys Arg Ala Ser Arg Asn Met Glu Ile Leu Lys Leu 
    130                 135                 140                 


Gly Gly Cys Glu Lys His Cys Lys Lys Lys Tyr Thr Ile Glu Lys Val 
145                 150                 155                 160 


Ile Lys Glu Val Gly Leu Leu Glu Lys Ser Lys Ser Val Met Pro Tyr 
                165                 170                 175     


Phe Phe Asn Glu Tyr Asp Leu Ile Lys Lys Arg Gln Gln Arg Arg Ala 
            180                 185                 190         


<210>  11
<211>  151
<212>  PRT
<213>  Helicobacter pylori

<400>  11

Met Asp Ser Glu Gly Phe Ser Pro Ser Ile Tyr Thr Asp Lys Thr Gly 
1               5                   10                  15      


His Pro Thr Ile Gly Tyr Gly Tyr Asn Leu Ser Val Tyr Ser Tyr Glu 
            20                  25                  30          


Ser Lys Arg Ile Thr Lys Pro Gln Ala Tyr Gly Leu Leu Thr Asp Ile 
        35                  40                  45              


Leu Lys Glu Asn His Lys Ala Leu Leu Ser Tyr Gly Trp Tyr Lys Asn 
    50                  55                  60                  


Leu Asp Ala Met Arg Arg Met Val Ile Leu Asp Leu Ser Tyr Asn Leu 
65                  70                  75                  80  


Gly Leu Ser Gly Leu Leu Lys Phe Lys Gln Phe Ile Lys Ala Ile Glu 
                85                  90                  95      


Asp Lys Asn Tyr Ala Leu Ala Val Glu Arg Leu Gln Lys Ser Pro Tyr 
            100                 105                 110         


Phe Asn Gln Val Arg Lys Arg Ala Ser Arg Asn Met Glu Ile Leu Lys 
        115                 120                 125             


Leu Gly Gly Cys Glu Lys His Cys Lys Lys Lys Tyr Thr Ile Glu Lys 
    130                 135                 140                 


Val Ile Lys Glu Gly Gly Ala 
145                 150     


<210>  12
<211>  8
<212>  PRT
<213>  Hepatitis C virus

<400>  12

Ser Gln Ser Arg Glu Ser Gln Cys 
1               5               


<210>  13
<211>  8
<212>  PRT
<213>  Hepatitis C virus

<400>  13

Cys Ser Gln Arg Gln Ser Glu Ser 
1               5               


<210>  14
<211>  1049
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized HP-Lysozyme inserted into E. coli expression 
       vector pET30

<400>  14
tagaataatt ttgtttaact ttaagaagga gatatacata tgcaccacca ccaccaccac       60

ggcggcgggg aattcatgga ttctgagggg ttttcccctt ctatttatac cgacaagact      120

gggcatccga ccattggcta tggttacaat ctgtctgttt attcttatga gggtaagcgt      180

atcaccaaaa cctatgggct tctgactgac atcctgtctt atgggtggta taaaaatctg      240

gacgcgatgc gtcgtatggt catcctggat ctgtcttaca atctgggcct gaacggtctg      300

ctcaaattca agcaattcat caaggcgatc gaggataaaa attatgctct ggctgttgag      360

cgtctgcaaa aatctccgta tttcaatcaa gtgaaaaaag agcgtcaagg tatctggaaa      420

ttttgactcg agcaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag      480

ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc      540

ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct      600

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg      660

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg      720

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac      780

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct      840

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt      900

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt      960

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt     1020

ttaacaaaat attaacgctt acaatttag                                       1049


<210>  15
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lysozyme protein sequence from DNA sequence given in SEQ ID NO:14

<400>  15

Met His His His His His His Gly Gly Gly Glu Phe Met Asp Ser Glu 
1               5                   10                  15      


Gly Phe Ser Pro Ser Ile Tyr Thr Asp Lys Thr Gly His Pro Thr Ile 
            20                  25                  30          


Gly Tyr Gly Tyr Asn Leu Ser Val Tyr Ser Tyr Glu Gly Lys Arg Ile 
        35                  40                  45              


Thr Lys Thr Tyr Gly Leu Leu Thr Asp Ile Leu Ser Tyr Gly Trp Tyr 
    50                  55                  60                  


Lys Asn Leu Asp Ala Met Arg Arg Met Val Ile Leu Asp Leu Ser Tyr 
65                  70                  75                  80  


Asn Leu Gly Leu Asn Gly Leu Leu Lys Phe Lys Gln Phe Ile Lys Ala 
                85                  90                  95      


Ile Glu Asp Lys Asn Tyr Ala Leu Ala Val Glu Arg Leu Gln Lys Ser 
            100                 105                 110         


Pro Tyr Phe Asn Gln Val Lys Lys Glu Arg Gln Gly Ile Trp Lys Phe 
        115                 120                 125             


