                         SEQUENCE LISTING

<110>  ɶ̼Ƽ޹˾
 
<120>  ׵׵ͻ塢׿׼Ӧ

<130>  PCT21119CN

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  292
<212>  PRT
<213>  Euryarchaeota archaeon

<400>  1

Met Asn Lys Ile Phe Ser Ile Ile Leu Val Ala Met Phe Leu Val Gly 
1               5                   10                  15      


Cys Thr Ala Thr Val Ser Asn Asn Thr Tyr Lys Lys His Tyr Ser Asp 
            20                  25                  30          


Ala Gly Val Gln Asn Ala Ser Gln Asp Val Lys Asn Phe Pro Lys Leu 
        35                  40                  45              


Asp Gly Pro Leu Ile Thr Val Ala Val Tyr Gln Phe Ser Asp Leu Thr 
    50                  55                  60                  


Gly Gln Arg Lys Pro Gly Gln Ile Ala His Leu Ser Ser Ala Val Thr 
65                  70                  75                  80  


Gln Gly Ala Gly Ala Tyr Leu Ile Glu Thr Leu Lys Glu Val Gly Asp 
                85                  90                  95      


Ser Ser Trp Phe Gln Val Val Glu Arg Thr Gly Ile Asp His Leu Ile 
            100                 105                 110         


Lys Glu Arg Gln Ile Ile Arg Gln Thr Arg Glu Leu Asn Lys Asp Gln 
        115                 120                 125             


Asp Val Leu Gln Pro Leu Leu Phe Ala Gly Val Leu Ile Glu Gly Ala 
    130                 135                 140                 


Ile Val Gly Tyr Asp Ser Asn Leu Glu Ser Gly Gly Tyr Gly Ala Arg 
145                 150                 155                 160 


Val Leu Gly Ile Gly Ala Asn Thr Gln Tyr Thr Arg Asp Thr Val Thr 
                165                 170                 175     


Val Ser Ile Arg Leu Val Ser Val Ser Ser Gly Glu Val Leu Leu Thr 
            180                 185                 190         


Ser Thr Thr Thr Lys Thr Ile Ile Ser Val Lys Thr Gln Gly Asp Val 
        195                 200                 205             


Phe Arg Trp Met Asp Ala Gly Thr Glu Pro Leu Glu Ala Glu Val Gly 
    210                 215                 220                 


Thr Ala Leu Asn Glu Pro Val Asn Val Ala Thr Arg Leu Ala Ile Glu 
225                 230                 235                 240 


Leu Ala Val Cys Asn Leu Ile Glu Lys Gly Lys Gln Lys Asn Leu Trp 
                245                 250                 255     


Ala Tyr Lys Lys Val Asn Glu Val Lys Glu Ser Lys Gln Glu Ile Lys 
            260                 265                 270         


Thr Glu Leu Lys Glu Glu Lys Val Ile Val Asp Ser Trp Val Pro Asp 
        275                 280                 285             


Thr Phe Asn Arg 
    290         


<210>  2
<211>  879
<212>  DNA
<213>  Euryarchaeota archaeon

<400>  2
atgaacaaga tattttcaat aatcctagtt gctatgttct tggtaggctg tacagcgaca       60

gttagtaata atacttacaa aaagcattat tctgacgctg gtgtacaaaa tgcctcacaa      120

gatgtcaaga actttccaaa gctagatggt ccactaatta ctgtagcagt atatcaattt      180

agtgacttaa ctggccaaag aaagcctgga caaatagcac atttaagctc agcagttacc      240

caaggtgctg gtgcttatct aatagaaact cttaaagaag taggagatag tagttggttc      300

caagttgttg aacgaactgg tattgatcac cttataaaag aaagacaaat tataagacaa      360

acaagagagc ttaataaaga ccaagatgta ctacaacctc tattgtttgc tggagtttta      420

atagaaggag ccatagttgg atacgacagc aaccttgaat caggagggta cggagctcga      480

gtgctaggca ttggagcaaa tactcaatat acaagagata cagtaacggt cagtattaga      540

ttagtatctg tatcaagcgg agaagtttta ttaacatcta ctacaacaaa aacaatcata      600

agtgtaaaaa cacaaggtga tgttttccgt tggatggatg ctggaacaga accacttgaa      660

gcagaagtag gaacggcact taatgaacca gtaaatgtag ctacaagact tgctattgaa      720

ttagcagtct gtaacttgat tgaaaaaggt aaacaaaaaa atctttgggc ttataagaaa      780

gtaaatgaag tgaaagaatc taaacaagaa ataaagacag aactaaaaga agaaaaagtc      840

attgttgata gttgggttcc ggacactttt aatagataa                             879


<210>  3
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  3
tttttttttt                                                              10


<210>  4
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  4
aatgtacttc gttcagttac gtattgct                                          28


<210>  5
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  5
gcaatacgta actgaacgaa gttcactatc gcattctcat ga                          42


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  6
tcatgagaat gcgatagtga                                                   20


<210>  7
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  7
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagcaatacg taactgaacg       60

aagtacatta aaaaaaaaaa aaaaaaaaa                                         89


<210>  8
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  8
atcctttttt tttttttttt tttt                                              24


<210>  9
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  9
aatgtacttc gttcagttac gtattgcttt tttttttttt tttttttt                    48


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  10
tttttttttt tttttttttt                                                   20


<210>  11
<211>  441
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  helicase

<400>  11

Met Ile Thr Ile Asp Gln Leu Thr Glu Gly Gln Phe Asp Ser Leu Gln 
1               5                   10                  15      


Arg Ala Lys Val Leu Ile Gln Glu Ala Thr Lys Asn Asp Gly Asn Trp 
            20                  25                  30          


Asn His Arg Thr Lys His Leu Thr Ile Asn Gly Pro Ala Gly Thr Gly 
        35                  40                  45              


Lys Thr Thr Met Met Lys Phe Leu Val Ser Trp Leu Arg Asp Glu Gly 
    50                  55                  60                  


Ile Thr Gly Val Ala Leu Ala Ala Pro Thr His Ala Ala Lys Lys Val 
65                  70                  75                  80  


Leu Ala Asn Ala Val Gly Glu Glu Val Ser Thr Ile His Ser Ile Leu 
                85                  90                  95      


Lys Ile Asn Pro Thr Thr Tyr Glu Cys Lys Gln Phe Phe Glu Gln Ser 
            100                 105                 110         


Ala Pro Pro Asp Leu Ser Lys Ile Arg Ile Leu Ile Cys Glu Glu Cys 
        115                 120                 125             


Ser Phe Tyr Asp Ile Lys Leu Phe Glu Ile Leu Met Asn Ser Ile Gln 
    130                 135                 140                 


Pro Trp Thr Ile Ile Ile Gly Ile Gly Asp Arg Ala Gln Leu Arg Pro 
145                 150                 155                 160 


Ala Asp Asp Lys Gly Ile Ser Arg Phe Phe Thr Asp Gln Arg Phe Glu 
                165                 170                 175     


Gln Thr Tyr Leu Thr Glu Ile Lys Arg Ser Asn Met Pro Ile Ile Glu 
            180                 185                 190         


Val Ala Thr Glu Ile Arg Asn Gly Gly Trp Ile Arg Glu Asn Ile Ile 
        195                 200                 205             


Asp Asp Leu Gly Val Lys Gln Asp Lys Ser Val Ser Glu Phe Met Thr 
    210                 215                 220                 


Asn Tyr Phe Lys Val Val Lys Ser Ile Asp Asp Leu Tyr Glu Thr Arg 
225                 230                 235                 240 


Met Tyr Ala Tyr Thr Asn Asn Ser Val Asp Thr Leu Asn Lys Ile Ile 
                245                 250                 255     


Arg Lys Lys Leu Tyr Glu Thr Glu Gln Asp Phe Ile Val Gly Glu Pro 
            260                 265                 270         


Ile Val Met Gln Glu Pro Leu Ile Arg Asp Ile Asn Tyr Glu Gly Lys 
        275                 280                 285             


Arg Phe Gln Glu Ile Val Phe Asn Asn Gly Glu Tyr Leu Glu Val Ser 
    290                 295                 300                 


Glu Ile Lys Pro Met Glu Ser Val Leu Lys Cys Arg Asn Ile Asp Tyr 
305                 310                 315                 320 


Gln Leu Val Leu His Tyr Tyr Gln Leu Lys Val Lys Ser Ile Asp Thr 
                325                 330                 335     


Gly Glu Ser Gly Leu Ile Asn Thr Ile Ser Asp Lys Asn Glu Leu Asn 
            340                 345                 350         


Lys Phe Tyr Met Phe Leu Gly Lys Val Cys Gln Asp Tyr Lys Ser Gly 
        355                 360                 365             


Thr Ile Lys Ala Phe Trp Asp Asp Phe Trp Lys Ile Lys Asn Asn Tyr 
    370                 375                 380                 


His Arg Val Lys Pro Leu Pro Val Ser Thr Ile His Lys Gly Gln Gly 
385                 390                 395                 400 


Ser Thr Val Asp Asn Ser Phe Leu Tyr Thr Pro Cys Ile Thr Lys Tyr 
                405                 410                 415     


Ala Glu Pro Asp Leu Ala Ser Gln Leu Leu Tyr Val Gly Val Thr Arg 
            420                 425                 430         


Ala Arg His Asn Val Asn Phe Val Gly 
        435                 440     


<210>  12
<211>  1326
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  helicase

<400>  12
atgatcacca tcgaccagct gaccgaaggt cagttcgact ctctgcagcg tgctaaagtt       60

ctgatccagg aagctaccaa aaacgacggt aactggaacc accgtaccaa acacctgacc      120

atcaacggtc cggctggtac cggtaaaacc accatgatga aattcctggt ttcttggctg      180

cgtgacgaag gtatcaccgg tgttgctctg gctgctccga cccacgctgc taaaaaagtt      240

ctggctaacg ctgttggtga agaagtttct accatccact ctatcctgaa aatcaacccg      300

accacctacg aatgcaaaca gttcttcgaa cagtctgctc cgccggacct gtctaaaatc      360

cgtatcctga tctgcgaaga atgctctttc tacgacatca aactgttcga aatcctgatg      420

aactctatcc agccgtggac catcatcatc ggtatcggtg accgtgctca gctgcgtccg      480

gctgacgaca aaggtatctc tcgtttcttc accgaccagc gtttcgaaca gacctacctg      540

accgaaatca aacgttctaa catgccgatc atcgaagttg ctaccgaaat ccgtaacggt      600

ggttggattc gtgaaaacat catcgacgac ctgggtgtta aacaggacaa atctgtttct      660

gaatttatga ccaactactt caaagttgtt aaatctatcg acgacctgta cgaaacccgt      720

atgtacgctt acaccaacaa ctctgttgac accctgaaca aaatcatccg taaaaaactg      780

tacgaaaccg aacaggactt catcgttggt gaaccgatcg ttatgcagga accgctgatc      840

cgtgacatca actacgaagg taaacgtttc caggaaatcg ttttcaacaa cggtgaatac      900

ctggaagttt ctgaaatcaa accgatggaa tctgttctga aatgccgtaa catcgactac      960

cagctggttc tgcactacta ccagctgaaa gttaaatcta tcgacaccgg tgaatctggt     1020

ctgatcaaca ccatctctga caaaaacgaa ctgaacaaat tctacatgtt cctgggtaaa     1080

gtttgccagg actacaaatc tggtaccatc aaagcgttct gggacgactt ctggaaaatc     1140

aaaaacaact accaccgtgt taaaccgctg ccggtttcta ccatccacaa aggtcagggt     1200

tctaccgttg acaactcttt cctgtacacc ccgtgcatca ccaaatacgc tgaaccggac     1260

ctggcttctc agctgctgta cgttggtgtt acccgtgctc gtcacaacgt taacttcgtt     1320

ggttaa                                                                1326


<210>  13
<211>  292
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 1

<400>  13

Met Asn Lys Ile Phe Ser Ile Ile Leu Val Ala Met Phe Leu Val Gly 
1               5                   10                  15      


Cys Thr Ala Thr Val Ser Asn Asn Thr Tyr Lys Lys His Tyr Ser Asp 
            20                  25                  30          


Ala Gly Val Gln Asn Ala Ser Gln Asp Val Lys Asn Phe Pro Lys Leu 
        35                  40                  45              


Asp Gly Pro Leu Ile Thr Val Ala Val Tyr Gln Phe Ser Asp Leu Thr 
    50                  55                  60                  


Gly Gln Arg Lys Pro Gly Gly Ile Ala Thr Leu Ser Thr Ala Val Thr 
65                  70                  75                  80  


Gln Gly Ala Gly Ala Tyr Leu Ile Glu Thr Leu Lys Glu Val Gly Asp 
                85                  90                  95      


Ser Ser Trp Phe Gln Val Val Glu Arg Thr Gly Ile Asn His Leu Ile 
            100                 105                 110         


Lys Glu Arg Gln Ile Ile Arg Gln Thr Arg Asn Leu Asn Lys Asp Gln 
        115                 120                 125             


Asn Val Leu Gln Pro Leu Leu Phe Ala Gly Val Leu Ile Glu Gly Ala 
    130                 135                 140                 


Ile Val Gly Tyr Asp Ser Asn Leu Glu Ser Gly Gly Tyr Gly Ala Arg 
145                 150                 155                 160 


Val Leu Gly Ile Gly Ala Asn Thr Gln Tyr Thr Arg Asp Thr Val Thr 
                165                 170                 175     


Val Ser Ile Arg Leu Val Ser Val Ser Ser Gly Glu Val Leu Leu Thr 
            180                 185                 190         


Ser Thr Thr Thr Lys Thr Ile Ile Ser Val Lys Thr Gln Gly Asp Val 
        195                 200                 205             


Phe Arg Trp Met Asp Ala Gly Thr Glu Pro Leu Glu Ala Glu Val Gly 
    210                 215                 220                 


Thr Ala Leu Asn Glu Pro Val Asn Val Ala Thr Arg Leu Ala Ile Glu 
225                 230                 235                 240 


Leu Ala Val Cys Asn Leu Ile Glu Lys Gly Lys Gln Lys Asn Leu Trp 
                245                 250                 255     


Ala Tyr Lys Lys Val Asn Glu Val Lys Glu Ser Lys Gln Glu Ile Lys 
            260                 265                 270         


Thr Glu Leu Lys Glu Glu Lys Val Ile Val Asp Ser Trp Val Pro Asp 
        275                 280                 285             


Thr Phe Asn Arg 
    290         


<210>  14
<211>  291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 2

<400>  14

Met Asn Lys Ile Phe Ser Ile Ile Leu Val Ala Met Phe Leu Val Gly 
1               5                   10                  15      


Cys Thr Ala Thr Val Ser Asn Asn Thr Tyr Lys Lys His Tyr Ser Asp 
            20                  25                  30          


Ala Gly Val Gln Asn Ala Ser Gln Asp Val Lys Asn Phe Pro Lys Leu 
        35                  40                  45              


Asp Gly Pro Leu Ile Thr Val Ala Val Tyr Gln Phe Ser Asp Leu Thr 
    50                  55                  60                  


Gly Gln Arg Lys Pro Gly Gln Ile Ala Gly Ser Ser Ala Val Thr Gln 
65                  70                  75                  80  


Gly Ala Gly Ala Tyr Leu Ile Glu Thr Leu Lys Glu Val Gly Asp Ser 
                85                  90                  95      


Ser Trp Phe Gln Val Val Glu Arg Thr Gly Ile Asp His Leu Ile Lys 
            100                 105                 110         


Glu Arg Gln Ile Ile Arg Gln Thr Arg Asn Leu Asn Lys Asp Gln Asn 
        115                 120                 125             


Val Leu Gln Pro Leu Leu Phe Ala Gly Val Leu Ile Glu Gly Ala Ile 
    130                 135                 140                 


Val Gly Tyr Asp Ser Asn Leu Glu Ser Gly Gly Tyr Gly Ala Arg Val 
145                 150                 155                 160 


Leu Gly Ile Gly Ala Asn Thr Gln Tyr Thr Arg Asp Thr Val Thr Val 
                165                 170                 175     


Ser Ile Arg Leu Val Ser Val Ser Ser Gly Glu Val Leu Leu Thr Ser 
            180                 185                 190         


Thr Thr Thr Lys Thr Ile Ile Ser Val Lys Thr Gln Gly Asp Val Phe 
        195                 200                 205             


Arg Trp Met Gly Ala Gly Thr Glu Pro Leu Glu Ala Glu Val Gly Thr 
    210                 215                 220                 


Ala Leu Asn Glu Pro Val Asn Val Ala Thr Arg Leu Ala Ile Glu Leu 
225                 230                 235                 240 


Ala Val Cys Asn Leu Ile Glu Lys Gly Lys Gln Lys Asn Leu Trp Ala 
                245                 250                 255     


Tyr Lys Lys Val Asn Glu Val Lys Glu Ser Lys Gln Glu Ile Lys Thr 
            260                 265                 270         


Glu Leu Lys Glu Glu Lys Val Ile Val Asp Ser Trp Val Pro Asp Thr 
        275                 280                 285             


Phe Asn Arg 
    290     


