﻿               SEQUENCE LISTING

<110> 北京蓝晶微生物科技有限公司
      深圳蓝晶生物科技有限公司

<120> 用于生产橄榄醇和橄榄醇酸的工程化微生物

<130> CIE220001PCT

<160> 15

<170> BiSSAP 1.3.6

<210> 1
<211> 6381
<212> DNA
<213> Artificial Sequence


<220> 
<223> p15A-Prha-OLS-OAC

<400> 1
agcggataac aatttcacac aggagagctc aaagagtgga acaatgcagg acgccgtccg       60

gtaacggtgt tgagcctcgg ttgtgtggtg attattgtcg ccgctaacat cgtcggcatc      120

ggcatggcga attaatcttt ctgcgaattg agatgacgcc actggctggg cgtcatcccg      180

gtttcccggg taaacaccac cgaaaaatag ttactatctt caaagccaca ttcggtcgaa      240

atatcactga ttaacaggcg gctatgctgg agaagatatt gcgcatgaca cactctgacc      300

tgtcgcagat attgattgat ggtcattcca gtctgctggc gaaattgctg acgcaaaacg      360

cgctcactgc acgatgcctc atcacaaaat ttatccagcg caaagggact tttcaggcta      420

gccgccagcc gggtaatcag cttatccagc aacgtttcgc tggatgttgg cggcaacgaa      480

tcactggtgt aacgatggcg attcagcaac atcaccaact gcccgaacag caactcagcc      540

atttcgttag caaacggcac atgctgacta ctttcatgct caagctgacc gataacctgc      600

cgcgcctgcg ccatccccat gctacctaag cgccagtgtg gttgccctgc gctggcgtta      660

aatcccggaa tcgccccctg ccagtcaaga ttcagcttca gacgctccgg gcaataaata      720

atattctgca aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc agcatgaatg      780

taaaagagat cgccacgggt aatgcgataa gggcgatcgt tgagtacatg caggccatta      840

ccgcgccaga caatcaccag ctcacaaaaa tcatgtgtat gttcagcaaa gacatcttgc      900

ggataacggt cagccacagc gactgcctgc tggtcgctgg caaaaaaatc atctttgaga      960

agttttaact gatgcgccac cgtggctacc tcggccagag aacgaagttg attattcgca     1020

atatggcgta caaatacgtt gagaagattc gcgttattgc agaaagccat cccgtccctg     1080

gcgaatatca cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa agtggttact     1140

gtcgctgaat ccacagcgat aggcgatgtc agtaacgctg gcctcgctgt ggcgtagcag     1200

atgtcgggct ttcatcagtc gcaggcggtt caggtatcgc tgaggcgtca gtcccgtttg     1260

ctgcttaagc tgccgatgta gcgtacgcag tgaaagagaa aattgatccg ccacggcatc     1320

ccaattcacc tcatcggcaa aatggtcctc cagccaggcc agaagcaagt tgagacgtga     1380

tgcgctgttt tccaggttct cctgcaaact gcttttacgc agcaagagca gtaattgcat     1440

aaacaagatc tcgcgactgg cggtcgaggg taaatcattt tccccttcct gctgttccat     1500

ctgtgcaacc agctgtcgca cctgctgcaa tacgctgtgg ttaacgcgcc agtgagacgg     1560

atactgccca tccagctctt gtggcagcaa ctgattcagc ccggcgagaa actgaaatcg     1620

atccggcgag cgatacagca cattggtcag acacagatta tcggtatgtt catacagatg     1680

ccgatcatga tcgcgtacga aacagaccgt gccaccggtg atggtatagg gctgcccatt     1740

aaacacatga atacccgtgc catgttcgac aatcacaatt tcatgaaaat catgatgatg     1800

ttcaggaaaa tccgcctgcg ggagccgggg ttctatcgcc acggacgcgt taccagacgg     1860

aaaaaaatcc acactatgta atacggtcat actggcctcc tgatgtcgtc aacacggcga     1920

aatagtaatc acgaggtcag gttcttacct taaattttcg acggaaaacc acgtaaaaaa     1980

cgtcgatttt tcaagataca gcgtgaattt tcaggaaatg cggtgagcat cacatcacca     2040

caattcagca aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt     2100

ttcctgtcag taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa     2160

ttcaactagt gctctgcagg agctgtcacc ggatgtgctt tccggtctga tgagtccgtg     2220

aggacgaaac agcctctaca aataattttg tttaagagtt actagagagg aggaattaac     2280

catgaaccat ctgcgtgcgg aaggccctgc gagcgtttta gcgattggca ccgcgaatcc     2340

ggaaaacatt ctgctgcagg atgaatttcc ggattattat tttcgcgtga ccaaaagcga     2400

acatatgacc cagctgaaag aaaaatttcg caaaatttgc gacaagagca tgattcgcaa     2460

acgcaactgc tttctgaacg aagaacatct gaaacagaac ccgcgcctgg tggaacatga     2520

aatgcagacc ctggatgcgc gccaggatat gctggtggtg gaagtgccga aactgggcaa     2580

agatgcgtgc gcgaaagcga ttaaagaatg gggccagccg aaaagcaaaa ttacccatct     2640

gatttttacc agcgcgagca ccaccgatat gccgggcgca gattatcatt gcgcgaaact     2700

gctgggcctg agcccgagcg ttaaacgcgt gatgatgtat cagctgggct gctatggcgg     2760

cggcaccgtt ttacgtattg cgaaagatat tgcggaaaac aacaaaggcg cgcgcgtgct     2820

ggcggtgtgt tgtgatatta tggcgtgcct gtttcgcggc ccgagcgaaa gcgatctgga     2880

actgttagtg ggccaggcga tttttggcga tggcgcggcg gcggtgattg tgggtgcaga     2940

acctgatgaa agcgtgggcg aacgccctat ttttgaactg gtgagcaccg gccagaccat     3000

tctgccgaat agcgaaggca ccattggcgg ccatattcgc gaagcgggcc tgatttttga     3060

tctgcataaa gatgtgccga tgctgattag caacaacatt gaaaaatgcc tgattgaggc     3120

gtttaccccg attggcatta gcgattggaa cagcatcttt tggattaccc atccgggcgg     3180

caaagcgatt ctggataaag tggaagaaaa actgcatctg aaaagcgata aattcgtgga     3240

tagccgccat gtgctgagcg aacatggcaa catgagcagc agcaccgtgc tgtttgtgat     3300

ggatgaactg cgcaaacgca gcctggaaga aggcaaaagc accaccggcg atggctttga     3360

atggggcgtg ctgtttggct ttggcccggg cttaaccgtg gaacgcgttg tggttcgtag     3420

cgtgcctatt aaatattaat actagagaaa gaggagaaat actagatggc ggtgaaacat     3480

ctgattgtgc tgaaatttaa agacgagatc accgaggcgc agaaagagga atttttcaaa     3540

acctatgtga acctggtgaa catcatcccg gcgatgaaag atgtgtattg gggcaaagat     3600

gtgacccaga aaaacaaaga agaaggctat acccatattg tggaagtgac ctttgaaagc     3660

gtggaaacca ttcaggatta tattattcac ccggcgcatg tgggctttgg cgatgtgtat     3720

cgcagctttt gggaaaaact gctgattttt gattacaccc cgcgcaaata actcgtcgtg     3780

actgggaaaa ccctggcgac tagtcttgga ctcctgttga tagatccagt aatgacctca     3840

gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag     3900

aatccagggg tccccaataa ttacgattta aattggcgaa aatgagacgt gggtctgacg     3960

ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct     4020

tcacctagat ccttttggtt catgtgcagc tccatcagca aaaggggatg ataagtttat     4080

caccaccgac tatttgcaac agcgccggtg atcgtgctat gatcgactga tgtcatcagc     4140

ggtggagtgc aatgtcgtgc aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct     4200

caaccttggg gttacccccg gcggtgtgct gctggtccac agctccttcc gtagcgtccg     4260

gcccctcgaa gatgggccac ttggactgat cgaggccctg cgtgctgcgc tgggtccggg     4320

agggacgctc gtcatgccct cgtggtcagg tctggacgac gagccgttcg atcctgccac     4380

gtcgcccgtt acaccggacc ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt     4440

aaagcgcagc gcccatccat ttgcctttgc ggcagcgggg ccacaggcag agcagatcat     4500

ctctgatcca ttgcccctgc cacctcactc gcctgcaagc ccggtcgccc gtgtccatga     4560

actcgatggg caggtacttc tcctcggcgt gggacacgat gccaacacga cgctgcatct     4620

tgccgagttg atggcaaagg ttccctatgg ggtgccgaga cactgcacca ttcttcagga     4680

tggcaagttg gtacgcgtcg attatctcga gaatgaccac tgctgtgagc gctttgcctt     4740

ggcggacagg tggctcaagg agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt     4800

tgctcggttg atccgctccc gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga     4860

tccgttgatc ttcctgcatc cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca     4920

gtcgattggc tgagctcatg agcggagaac gagatgacgt tggaggggca aggtcgcgct     4980

gattgctggg gcaacacgtg gagcggatcg gtttgacttt tgtccttttc cgctgcataa     5040

ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat     5100

acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg     5160

ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat     5220

tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgta ggccggccgc     5280

gatgcaggtg gctgctgaac ccccagccgg aactgacccc acaaggccct agcggagtgt     5340

atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga     5400

gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct     5460

cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac     5520

ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc     5580

ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc     5640

aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc ccctggcggc     5700

tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg     5760

ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct     5820

ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg     5880

tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg     5940

atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt     6000

ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa     6060

ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca     6120

gaccaaaacg atctcaagaa gatcatctta ttaactacat ggctctgctg tagtgagtgg     6180

gttgcgctcc ggcagcggtc ctgatccccc gcagaaaaaa aggatctcaa gaagatcctt     6240

tgatcttttc tacggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag     6300

cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt     6360

ttatttgatg cctttaatta a                                               6381


<210> 2
<211> 1158
<212> DNA
<213> Cannabis sativa


<220> 
<223> OLS编码序列

<400> 2
atgaaccatc tgcgtgcgga aggccctgcg agcgttttag cgattggcac cgcgaatccg      60

gaaaacattc tgctgcagga tgaatttccg gattattatt ttcgcgtgac caaaagcgaa     120

catatgaccc agctgaaaga aaaatttcgc aaaatttgcg acaagagcat gattcgcaaa     180

cgcaactgct ttctgaacga agaacatctg aaacagaacc cgcgcctggt ggaacatgaa     240

atgcagaccc tggatgcgcg ccaggatatg ctggtggtgg aagtgccgaa actgggcaaa     300

gatgcgtgcg cgaaagcgat taaagaatgg ggccagccga aaagcaaaat tacccatctg     360

atttttacca gcgcgagcac caccgatatg ccgggcgcag attatcattg cgcgaaactg     420

ctgggcctga gcccgagcgt taaacgcgtg atgatgtatc agctgggctg ctatggcggc     480

ggcaccgttt tacgtattgc gaaagatatt gcggaaaaca acaaaggcgc gcgcgtgctg     540

gcggtgtgtt gtgatattat ggcgtgcctg tttcgcggcc cgagcgaaag cgatctggaa     600

ctgttagtgg gccaggcgat ttttggcgat ggcgcggcgg cggtgattgt gggtgcagaa     660

cctgatgaaa gcgtgggcga acgccctatt tttgaactgg tgagcaccgg ccagaccatt     720

ctgccgaata gcgaaggcac cattggcggc catattcgcg aagcgggcct gatttttgat     780

ctgcataaag atgtgccgat gctgattagc aacaacattg aaaaatgcct gattgaggcg     840

tttaccccga ttggcattag cgattggaac agcatctttt ggattaccca tccgggcggc     900

aaagcgattc tggataaagt ggaagaaaaa ctgcatctga aaagcgataa attcgtggat     960

agccgccatg tgctgagcga acatggcaac atgagcagca gcaccgtgct gtttgtgatg    1020

gatgaactgc gcaaacgcag cctggaagaa ggcaaaagca ccaccggcga tggctttgaa    1080

tggggcgtgc tgtttggctt tggcccgggc ttaaccgtgg aacgcgttgt ggttcgtagc    1140

gtgcctatta aatattaa                                                  1158


<210> 3
<211> 385
<212> PRT
<213> Cannabis sativa


<220> 
<223> OLS氨基酸序列

<400> 3
Met Asn His Leu Arg Ala Glu Gly Pro Ala Ser Val Leu Ala Ile Gly 
1               5                   10                  15      
Thr Ala Asn Pro Glu Asn Ile Leu Leu Gln Asp Glu Phe Pro Asp Tyr 
            20                  25                  30          
Tyr Phe Arg Val Thr Lys Ser Glu His Met Thr Gln Leu Lys Glu Lys 
        35                  40                  45              
Phe Arg Lys Ile Cys Asp Lys Ser Met Ile Arg Lys Arg Asn Cys Phe 
    50                  55                  60                  
Leu Asn Glu Glu His Leu Lys Gln Asn Pro Arg Leu Val Glu His Glu 
65                  70                  75                  80  
Met Gln Thr Leu Asp Ala Arg Gln Asp Met Leu Val Val Glu Val Pro 
                85                  90                  95      
Lys Leu Gly Lys Asp Ala Cys Ala Lys Ala Ile Lys Glu Trp Gly Gln 
            100                 105                 110         
Pro Lys Ser Lys Ile Thr His Leu Ile Phe Thr Ser Ala Ser Thr Thr 
        115                 120                 125             
Asp Met Pro Gly Ala Asp Tyr His Cys Ala Lys Leu Leu Gly Leu Ser 
    130                 135                 140                 
Pro Ser Val Lys Arg Val Met Met Tyr Gln Leu Gly Cys Tyr Gly Gly 
145                 150                 155                 160 
Gly Thr Val Leu Arg Ile Ala Lys Asp Ile Ala Glu Asn Asn Lys Gly 
                165                 170                 175     
Ala Arg Val Leu Ala Val Cys Cys Asp Ile Met Ala Cys Leu Phe Arg 
            180                 185                 190         
Gly Pro Ser Glu Ser Asp Leu Glu Leu Leu Val Gly Gln Ala Ile Phe 
        195                 200                 205             
Gly Asp Gly Ala Ala Ala Val Ile Val Gly Ala Glu Pro Asp Glu Ser 
    210                 215                 220                 
Val Gly Glu Arg Pro Ile Phe Glu Leu Val Ser Thr Gly Gln Thr Ile 
225                 230                 235                 240 
Leu Pro Asn Ser Glu Gly Thr Ile Gly Gly His Ile Arg Glu Ala Gly 
                245                 250                 255     
Leu Ile Phe Asp Leu His Lys Asp Val Pro Met Leu Ile Ser Asn Asn 
            260                 265                 270         
Ile Glu Lys Cys Leu Ile Glu Ala Phe Thr Pro Ile Gly Ile Ser Asp 
        275                 280                 285             
Trp Asn Ser Ile Phe Trp Ile Thr His Pro Gly Gly Lys Ala Ile Leu 
    290                 295                 300                 
Asp Lys Val Glu Glu Lys Leu His Leu Lys Ser Asp Lys Phe Val Asp 
305                 310                 315                 320 
Ser Arg His Val Leu Ser Glu His Gly Asn Met Ser Ser Ser Thr Val 
                325                 330                 335     
Leu Phe Val Met Asp Glu Leu Arg Lys Arg Ser Leu Glu Glu Gly Lys 
            340                 345                 350         
Ser Thr Thr Gly Asp Gly Phe Glu Trp Gly Val Leu Phe Gly Phe Gly 
        355                 360                 365             
Pro Gly Leu Thr Val Glu Arg Val Val Val Arg Ser Val Pro Ile Lys 
    370                 375                 380                 
Tyr 
385 

<210> 4
<211> 306
<212> DNA
<213> Cannabis sativa


<220> 
<223> OAC编码序列

<400> 4
atggcggtga aacatctgat tgtgctgaaa tttaaagacg agatcaccga ggcgcagaaa     60

gaggaatttt tcaaaaccta tgtgaacctg gtgaacatca tcccggcgat gaaagatgtg    120

tattggggca aagatgtgac ccagaaaaac aaagaagaag gctataccca tattgtggaa    180

gtgacctttg aaagcgtgga aaccattcag gattatatta ttcacccggc gcatgtgggc    240

tttggcgatg tgtatcgcag cttttgggaa aaactgctga tttttgatta caccccgcgc    300

aaataa                                                               306


<210> 5
<211> 101
<212> PRT
<213> Cannabis sativa


<220> 
<223> OAC氨基酸序列

<400> 5
Met Ala Val Lys His Leu Ile Val Leu Lys Phe Lys Asp Glu Ile Thr 
1               5                   10                  15      
Glu Ala Gln Lys Glu Glu Phe Phe Lys Thr Tyr Val Asn Leu Val Asn 
            20                  25                  30          
Ile Ile Pro Ala Met Lys Asp Val Tyr Trp Gly Lys Asp Val Thr Gln 
        35                  40                  45              
Lys Asn Lys Glu Glu Gly Tyr Thr His Ile Val Glu Val Thr Phe Glu 
    50                  55                  60                  
Ser Val Glu Thr Ile Gln Asp Tyr Ile Ile His Pro Ala His Val Gly 
65                  70                  75                  80  
Phe Gly Asp Val Tyr Arg Ser Phe Trp Glu Lys Leu Leu Ile Phe Asp 
                85                  90                  95      
Tyr Thr Pro Arg Lys 
            100     

<210> 6
<211> 2353
<212> DNA
<213> Artificial Sequence


<220> 
<223> H1-kana-H2

<400> 6
ccttctatca attatatcgg ctatcttgaa gccaatgagt tgttaactgg caagacagat      60

gtgctggttt gtgacggctt tacaggaaat gtcacattaa agacgatgga aggtgttgtc     120

aggatgttcc tttctctgct gaaatctcag ggtgaaggga aaaaacggtc gtggtggcta     180

ctgttattaa agcgttggct acaaaagagc ctgacgaggc gattcagtca cctcaacccc     240

gaccagtata acggcgcctg tctgttagga ttgcgcggca cggtgataaa aagtcatggt     300

gcagccaatc agcgagcttt tgcggtcgcg attgaacagg cagtgcaggc ggtgcagcga     360

caagttcctc agcgaattgc cgctcgcctg gaatctgtat acccagctgg ttttgagctg     420

ctggacggtg gcaaaagcgg aactctgcgg tagcaggacg ctgccagcga actcgcagtt     480

tgcaagtgac ggtatataac cgaaaagtga ctgagcgtca tgattccggg gatccgtcga     540

cctgcagttc gaagttccta ttctctagaa agtataggaa cttcggatga atgtcagcta     600

ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg     660

gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc     720

agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt     780

gccgccaagg atctgatggc gcaggggatc aagatctgat caagagacag gatgaggatc     840

gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag     900

gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg     960

gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa    1020

tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc    1080

agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc    1140

ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga    1200

tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa    1260

acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct    1320

ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat    1380

gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt    1440

ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta    1500

tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga    1560

ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg    1620

ccttcttgac gagttcttct gagaagttcc tattctctag aaagtatagg aacttcgaag    1680

cagctccagc ctacatccgc gctggttcgt ttctaggata aggattaaaa catgacgcaa    1740

tttgcatttg tgttccctgg acagggttct caaaccgttg gaatgctggc tgatatggcg    1800

gcgagctatc caattgtcga agaaacgttt gctgaagctt ctgcggcgct gggctacgac    1860

ctgtgggcgc tgacccagca ggggccagct gaagaactga ataaaacctg gcaaactcag    1920

cctgcgctgt tgactgcatc tgttgcgctg tatcgcgtat ggcagcagca gggcggtaaa    1980

gcaccggcaa tgatggccgg tcacagcctg ggggaatact ccgcgctggt ttgcgctggt    2040

gtgattgatt tcgctgatgc ggtgcgtctg gttgagatgc gcggcaagtt catgcaagaa    2100

gccgtaccgg aaggcacggg cgctatggcg gcaatcatcg gtctggatga tgcgtctatt    2160

gcgaaagcgt gtgaagaagc tgcagaaggt caggtcgttt ctccggtaaa ctttaactct    2220

ccgggacagg tggttattgc cggtcataaa gaagcggttg agcgtgctgg cgctgcctgt    2280

aaagcggcgg gcgcaaaacg cgcgctgccg ttaccagtga gcgtaccgtc tcactgtgcg    2340

ctgatgaaac cag                                                       2353


<210> 7
<211> 1261
<212> DNA
<213> Artificial Sequence


<220> 
<223> 缺失fabH基因的BW25113基因组序列中在原fabH基因位点处上下游的部分序列

<400> 7
ccttctatca attatatcgg ctatcttgaa gccaatgagt tgttaactgg caagacagat      60

gtgctggttt gtgacggctt tacaggaaat gtcacattaa agacgatgga aggtgttgtc     120

aggatgttcc tttctctgct gaaatctcag ggtgaaggga aaaaacggtc gtggtggcta     180

ctgttattaa agcgttggct acaaaagagc ctgacgaggc gattcagtca cctcaacccc     240

gaccagtata acggcgcctg tctgttagga ttgcgcggca cggtgataaa aagtcatggt     300

gcagccaatc agcgagcttt tgcggtcgcg attgaacagg cagtgcaggc ggtgcagcga     360

caagttcctc agcgaattgc cgctcgcctg gaatctgtat acccagctgg ttttgagctg     420

ctggacggtg gcaaaagcgg aactctgcgg tagcaggacg ctgccagcga actcgcagtt     480

tgcaagtgac ggtatataac cgaaaagtga ctgagcgtca tgattccggg gatccgtcga     540

cctgcagttc gaagttccta ttctctagaa agtataggaa cttcgaagca gctccagcct     600

acatccgcgc tggttcgttt ctaggataag gattaaaaca tgacgcaatt tgcatttgtg     660

ttccctggac agggttctca aaccgttgga atgctggctg atatggcggc gagctatcca     720

attgtcgaag aaacgtttgc tgaagcttct gcggcgctgg gctacgacct gtgggcgctg     780

acccagcagg ggccagctga agaactgaat aaaacctggc aaactcagcc tgcgctgttg     840

actgcatctg ttgcgctgta tcgcgtatgg cagcagcagg gcggtaaagc accggcaatg     900

atggccggtc acagcctggg ggaatactcc gcgctggttt gcgctggtgt gattgatttc     960

gctgatgcgg tgcgtctggt tgagatgcgc ggcaagttca tgcaagaagc cgtaccggaa    1020

ggcacgggcg ctatggcggc aatcatcggt ctggatgatg cgtctattgc gaaagcgtgt    1080

gaagaagctg cagaaggtca ggtcgtttct ccggtaaact ttaactctcc gggacaggtg    1140

gttattgccg gtcataaaga agcggttgag cgtgctggcg ctgcctgtaa agcggcgggc    1200

gcaaaacgcg cgctgccgtt accagtgagc gtaccgtctc actgtgcgct gatgaaacca    1260

g                                                                    1261


<210> 8
<211> 954
<212> DNA
<213> Escherichia coli


<220> 
<223> 被缺失的fabH基因序列

<400> 8
atgtatacga agattattgg tactggcagc tatctgcccg aacaagtgcg gacaaacgcc      60

gatttggaaa aaatggtgga cacctctgac gagtggattg tcactcgtac cggtatccgc     120

gaacgccaca ttgccgcgcc aaacgaaacc gtttcaacca tgggctttga agcggcgaca     180

cgcgcaattg agatggcggg cattgagaaa gaccagattg gcctgatcgt tgtggcaacg     240

acttctgcta cgcacgcttt cccgagcgca gcttgtcaga ttcaaagcat gttgggcatt     300

aaaggttgcc cggcatttga cgttgcagca gcctgcgcag gtttcaccta tgcattaagc     360

gtagccgatc aatacgtgaa atctggggcg gtgaagtatg ctctggtcgt cggttccgat     420

gtactggcgc gcacctgcga tccaaccgat cgtgggacta ttattatttt tggcgatggc     480

gcgggcgctg cggtgctggc tgcctctgaa gagccgggaa tcatttccac ccatctgcat     540

gccgacggta gttatggtga attgctgacg ctgccaaacg ccgaccgcgt gaatccagag     600

aattcaattc atctgacgat ggcgggcaac gaagtcttca aggttgcggt aacggaactg     660

gcgcacatcg ttgatgagac gctggcggcg aataatcttg accgttctca actggactgg     720

ctggttccgc atcaggctaa cctgcgtatt atcagtgcaa cggcgaaaaa actcggtatg     780

tctatggata atgtcgtggt gacgctggat cgccacggta atacctctgc ggcctctgtc     840

ccgtgcgcgc tggatgaagc tgtacgcgac gggcgcatta agccggggca gttggttctg     900

cttgaagcct ttggcggtgg attcacctgg ggctccgcgc tggttcgttt ctag           954


<210> 9
<211> 2161
<212> DNA
<213> Artificial Sequence


<220> 
<223> H3-kana-H4

<400> 9
tcattaccga cgcaggaaat atgactaacg tcagaaatag caatcgccgg gtagcccgga      60

cggttttcac ggtagcgacc ggtcaactct tcggcaaagt gcatagcgtc gcaatgggaa     120

ccgccgttgc cgcaggaaag cactttgcca ccggctttaa agctgtctgc taacaggacc     180

gccgcgcgct gaatggcgtg aatattggcg tcatctttta aaaagttagc cagcgtttcc     240

gccgcttcgt tcagttcgtt acgaataaga tcctggtaca tgaggatatc cttcagcata     300

aatgtaatag acaaaatgca gtgtaccgga taccgccaaa agcgagaagt acgggcaggt     360

gctatgacca ggactttttg acctgaagtg cggataaaaa cagcaacaat gtgagctttg     420

ttgtaattat attgtaaaca tattgctaaa tgtttttaca tccactacaa ccatatcatc     480

acaagtggtc agacctccta caagtaaggg gcttttcgtt gaagttccta ttctctagaa     540

agtataggaa cttcggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag     600

cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt     660

tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa     720

gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc     780

aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca     840

cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac     900

aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt     960

tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc    1020

gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg    1080

aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc    1140

tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc    1200

ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat    1260

ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc    1320

cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca    1380

tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga    1440

ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat    1500

tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc    1560

tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagaagttcc    1620

tattctctag aaagtatagg aacttcatga ataacggagc cgaaaggctc cgtttcttta    1680

tccgctaatt atttaaaatt aaagccatcc ggatggtttt ccaggctgcc ggtcaacgcc    1740

gcgaacaaca ccgttttacc atcaatcgaa agcgcatcgt tcacattcag ccaggtgagt    1800

ttctcttgcg acgttttctc atcaatagtc gagaacagcc ccgtcatctg attagatttc    1860

tcggaccaca tcacagcgat acgttgcgag ccacagtcat gcggtttgca cgcgctcatc    1920

acctgatacg tctcatctcc caacgttacg gtttgtgcgg gagtataagt accgcctttc    1980

atcacccagg caggcagctt atgcccttgt accatctgat taaatgcagc tttggtggtt    2040

tcgccctttg caaggctgct aatggttaaa tcatcctgcg ccattgcact ggtggcgatg    2100

accagagcgg cgactgtcgt tattgcctta aacatcattc ctcccgagct tatcctgccc    2160

a                                                                    2161


<210> 10
<211> 1015
<212> DNA
<213> Artificial Sequence


<220> 
<223> 缺失fadE基因的BW25113基因组序列中在原fadE基因位点处上下游的部分序列

<400> 10
caaagtgcat agcgtcgcaa tgggaaccgc cgttgccgca ggaaagcact ttgccaccgg      60

ctttaaagct gtctgctaac aggaccgccg cgcgctgaat ggcgtgaata ttggcgtcat     120

cttttaaaaa gttagccagc gtttccgccg cttcgttcag ttcgttacga ataagatcct     180

ggtacatgag gatatccttc agcataaatg taatagacaa aatgcagtgt accggatacc     240

gccaaaagcg agaagtacgg gcaggtgcta tgaccaggac tttttgacct gaagtgcgga     300

taaaaacagc aacaatgtga gctttgttgt aattatattg taaacatatt gctaaatgtt     360

tttacatcca ctacaaccat atcatcacaa gtggtcagac ctcctacaag taaggggctt     420

ttcgttaccg tttaaataat gccaattatt taaagttagc ggccgcgaag ttcctattct     480

ctagaaagta taggaacttc atgaataacg gagccgaaag gctccgtttc tttatccgct     540

aattatttaa aattaaagcc atccggatgg ttttccaggc tgccggtcaa cgccgcgaac     600

aacaccgttt taccatcaat cgaaagcgca tcgttcacat tcagccaggt gagtttctct     660

tgcgacgttt tctcatcaat agtcgagaac agccccgtca tctgattaga tttctcggac     720

cacatcacag cgatacgttg cgagccacag tcatgcggtt tgcacgcgct catcacctga     780

tacgtctcat ctcccaacgt tacggtttgt gcgggagtat aagtaccgcc tttcatcacc     840

caggcaggca gcttatgccc ttgtaccatc tgattaaatg cagctttggt ggtttcgccc     900

tttgcaaggc tgctaatggt taaatcatcc tgcgccattg cactggtggc gatgaccaga     960

gcggcgactg tcgttattgc cttaaacatc attcctcccg agcttatcct gccca         1015


<210> 11
<211> 2445
<212> DNA
<213> Escherichia coli


<220> 
<223> 被缺失的fadE基因序列

<400> 11
atgatgattt tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc      60

gtgagcttat ttatcagcag tctgattttg ctcgcctgga cagccgccct cggcgttgct     120

ggtctgtggt cggcgtgggt actggtgcct ctggccatta tcctcgtgcc atttaacttt     180

gcgcctatgc gtaagtcgat gatttccgcg ccggtatttc gcggtttccg taaggtgatg     240

ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg gcaccacctg gtgggagggc     300

gacttgttcc agggcaagcc ggactggaaa aagctgcata actatccgca gccgcgcctg     360

accgccgaag agcaagcgtt tctcgacggc ccggtagaag aagcctgccg gatggcgaat     420

gatttccaga tcacccatga gctggcggat ctgccgccgg agttgtgggc gtaccttaaa     480

gagcatcgtt tcttcgcgat gatcatcaaa aaagagtacg gcgggctgga gttctcggct     540

tatgcccagt ctcgcgtgct gcaaaaactc tccggcgtga gcgggatcct ggcgattacc     600

gtcggcgtgc caaactcatt aggcccgggc gaactgttgc aacattacgg cactgacgag     660

cagaaagatc actatctgcc gcgtctggcg cgtggtcagg agatcccctg ctttgcactg     720

accagcccgg aagcgggttc cgatgcgggc gcgattccgg acaccgggat tgtctgcatg     780

ggcgaatggc agggccagca ggtgctgggg atgcgtctga cctggaacaa acgctacatt     840

acgctggcac cgattgcgac cgtgcttggg ctggcgttta aactctccga cccggaaaaa     900

ttactcggcg gtgcagaaga tttaggcatt acctgtgcgc tgatcccaac caccacgccg     960

ggcgtggaaa ttggtcgtcg ccacttcccg ctgaacgtac cgttccagaa cggaccgacg    1020

cgcggtaaag atgtcttcgt gccgatcgat tacatcatcg gcgggccgaa aatggccggg    1080

caaggctggc ggatgctggt ggagtgcctc tcggtaggcc gcggcatcac cctgccttcc    1140

aactcaaccg gcggcgtgaa atcggtagcg ctggcaaccg gcgcgtatgc tcacattcgc    1200

cgtcagttca aaatctctat tggtaagatg gaagggattg aagagccgct ggcgcgtatt    1260

gccggtaatg cctacgtgat ggatgctgcg gcatcgctga ttacctacgg cattatgctc    1320

ggcgaaaaac ctgccgtgct gtcggctatc gttaagtatc actgtaccca ccgcgggcag    1380

cagtcgatta ttgatgcgat ggatattacc ggcggtaaag gcattatgct cgggcaaagc    1440

aacttcctgg cgcgtgctta ccagggcgca ccgattgcca tcaccgttga aggggctaac    1500

attctgaccc gcagcatgat gatcttcgga caaggagcga ttcgttgcca tccgtacgtg    1560

ctggaagaga tggaagcggc gaagaacaat gacgtcaacg cgttcgataa actgttgttc    1620

aaacatatcg gtcacgtcgg tagcaacaaa gttcgcagct tctggctggg cctgacgcgc    1680

ggtttaacca gcagcacgcc aaccggcgat gccactaaac gctactatca gcacctgaac    1740

cgcctgagcg ccaacctcgc cctgctttct gatgtctcga tggcagtgct gggcggcagc    1800

ctgaaacgtc gcgagcgcat ctcggcccgt ctgggggata ttttaagcca gctctacctc    1860

gcctctgccg tgctgaagcg ttatgacgac gaaggccgta atgaagccga cctgccgctg    1920

gtgcactggg gcgtacaaga tgcgctgtat caggctgaac aggcgatgga tgatttactg    1980

caaaacttcc cgaaccgcgt ggttgccggg ctgctgaatg tggtgatctt cccgaccgga    2040

cgtcattatc tggcaccttc tgacaagctg gatcataaag tggcgaagat tttacaagtg    2100

ccgaacgcca cccgttcccg cattggtcgc ggtcagtacc tgacgccgag cgagcataat    2160

ccggttggct tgctggaaga ggcgctggtg gatgtgattg ccgccgaccc aattcatcag    2220

cggatctgta aagagctggg taaaaacctg ccgtttaccc gtctggatga actggcgcac    2280

aacgcgctgg tgaaggggct gattgataaa gatgaagccg ctattctggt gaaagctgaa    2340

gaaagccgtc tgcgcagtat taacgttgat gactttgatc cggaagagct ggcgacgaag    2400

ccggtaaagt tgccggagaa agtgcggaaa gttgaagccg cgtaa                    2445


<210> 12
<211> 6563
<212> DNA
<213> Artificial Sequence


<220> 
<223> pL-Prha-fadD

<400> 12
tcgtcgacct aattcccatg tcagccgtta agtgttcctg tgtcactcaa aattgctttg       60

agaggctcta agggcttctc agtgcgttac atccctggct tgttgtccac aaccgttaaa      120

ccttaaaagc tttaaaagcc ttatatattc ttttttttct tataaaactt aaaaccttag      180

aggctattta agttgctgat ttatattaat tttattgttc aaacatgaga gcttagtacg      240

tgaaacatga gagcttagta cgttagccat gagagcttag tacgttagcc atgagggttt      300

agttcgttaa acatgagagc ttagtacgtt aaacatgaga gcttagtacg tgaaacatga      360

gagcttagta cgtactatca acaggttgaa ctgcggatct tgatgagtgg atagtacgtt      420

gctaaaacat gagataaaaa ttgactctca tgttattggc gttaagatat acagaatgat      480

gaggtttttt tatgagactc aaggtcatga tggacgtgaa caaaaaaacg aaaattcgcc      540

accgaaacga gctaaatcac accctggctc aacttccttt gcccgcaaag cgagtgatgt      600

atatggcgct tgctcccatt gatagcaagg aacctcttga acgagggcga gttttcaaaa      660

ttagggctga agaccttgca gcgctcgcca aaatcacccc atcgcttgct tatcgacaat      720

taaaagaggg tggtaagtta cttggtgcca gcaaaatttc gctaagaggg gatgatatca      780

ttgcttcagc taaagagctt aacctgctct ttactgctaa agactcccct gaagagttag      840

atcttaacat tattgagtgg atagcttatt caaatgatga aggatacttg tctttaaaat      900

tcaccagaac catagaacca tatatctcta gccttattgg gaaaaaaaat aaattcacaa      960

cgcaattgtt aacggcaagc ttacgcttaa gtagccagta ttcatcttct ctttatcaac     1020

ttatcaggaa gcattactct aattttaaga agaaaaatta ttttattatt tccgttgatg     1080

agttaaagga agagttaata gcttatactt ttgataaaga tggaagtatt gagtacaaat     1140

accctgactt tcctattttt aaaagggatg tattaaataa agccattgct gaaattaaaa     1200

agaaaacaga aatatcgttt gttggcttta ctgttcatga aaaagaagga agaaaaatta     1260

gtaagctgaa gttcgaattt gtcgttgatg aagatgaatt ttctggcgat aaagatgatg     1320

aagctttttt tatgaattta tctgaagcta atgcagcttt tctcaaggta tttgatgaaa     1380

ccgtacctcc caaaaaagct aaggggtgat atatggctaa aatttacgat ttccctcaag     1440

gagccgaacg ccgcaggatg caccgcaaaa tccagtggaa caacgctgta aaattatcta     1500

aaaatggctg gagtaagcca gaggttaaac gctggtcttt tttagcattc atctcaactg     1560

gctgcggccg cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag     1620

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gggaagcggt     1680

gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga     1740

accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca     1800

cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc     1860

tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc     1920

tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg     1980

cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc     2040

cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt     2100

ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc     2160

gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa     2220

tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa     2280

ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact     2340

tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca     2400

gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg     2460

tctaacaatt cgttcaagcc gaggggccgc aagatccggc cacgatgacc cggtcgtcgg     2520

ttcagggcag ggtcgttaaa tagccgctta tgtctattgc tggtttaccg gtttattgac     2580

taccggaagc agagcggata acaatttcac acaggagagc tcaaagagtg gaacaatgca     2640

ggacgccgtc cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac     2700

atcgtcggca tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg     2760

ggcgtcatcc cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca     2820

cattcggtcg aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga     2880

cacactctga cctgtcgcag atattgattg atggtcattc cagtctgctg gcgaaattgc     2940

tgacgcaaaa cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga     3000

cttttcaggc tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt     3060

ggcggcaacg aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac     3120

agcaactcag ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga     3180

ccgataacct gccgcgcctg cgccatcccc atgctaccta agcgccagtg tggttgccct     3240

gcgctggcgt taaatcccgg aatcgccccc tgccagtcaa gattcagctt cagacgctcc     3300

gggcaataaa taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg     3360

tcagcatgaa tgtaaaagag atcgccacgg gtaatgcgat aagggcgatc gttgagtaca     3420

tgcaggccat taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcagca     3480

aagacatctt gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa     3540

tcatctttga gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt     3600

tgattattcg caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc     3660

atcccgtccc tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga     3720

aaagtggtta ctgtcgctga atccacagcg ataggcgatg tcagtaacgc tggcctcgct     3780

gtggcgtagc agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt     3840

cagtcccgtt tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc     3900

cgccacggca tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa     3960

gttgagacgt gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag     4020

cagtaattgc ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc     4080

ctgctgttcc atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg     4140

ccagtgagac ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag     4200

aaactgaaat cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg     4260

ttcatacaga tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata     4320

gggctgccca ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa     4380

atcatgatga tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc     4440

gttaccagac ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg     4500

tcaacacggc gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa     4560

ccacgtaaaa aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc     4620

atcacatcac cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc     4680

caatggccca ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg     4740

gtcgtaatga aattcaacta gtgctctgca ggagctgtca ccggatgtgc tttccggtct     4800

gatgagtccg tgaggacgaa acagcctcta caaataattt tgtttaagag ttactagaga     4860

aagaggagaa atactagttg aagaaggttt ggcttaaccg ttatcccgcg gacgttccga     4920

cggagatcaa ccctgaccgt tatcaatctc tggtagatat gtttgagcag tcggtcgcgc     4980

gctacgccga tcaacctgcg tttgtgaata tgggggaggt aatgaccttc cgcaagctgg     5040

aagaacgcag tcgcgcgttt gccgcttatt tgcaacaagg gttggggctg aagaaaggcg     5100

atcgcgttgc gttgatgatg cctaatttat tgcaatatcc ggtggcgctg tttggcattt     5160

tgcgtgccgg gatgatcgtc gtaaacgtta acccgttgta taccccgcgt gagcttgagc     5220

atcagcttaa cgatagcggc gcatcggcga ttgttatcgt gtctaacttt gctcacacac     5280

tggaaaaagt ggttgataaa accgccgttc agcacgtaat tctgacccgt atgggcgatc     5340

agctatctac ggcaaaaggc acggtagtca atttcgttgt taaatacatc aagcgtttgg     5400

tgccgaaata ccatctgcca gatgccattt catttcgtag cgcactgcat aacggctacc     5460

ggatgcagta cgtcaaaccc gaactggtgc cggaagattt agcttttctg caatacaccg     5520

gcggcaccac tggtgtggcg aaaggcgcga tgctgactca ccgcaatatg ctggcgaacc     5580

tggaacaggt taacgcgacc tatggtccgc tgttgcatcc gggcaaagag ctggtggtga     5640

cggcgctgcc gctgtatcac atttttgccc tgaccattaa ctgcctgctg tttatcgaac     5700

tgggtgggca gaacctgctt atcactaacc cgcgcgatat tccagggttg gtaaaagagt     5760

tagcgaaata tccgtttacc gctatcacgg gcgttaacac cttgttcaat gcgttgctga     5820

acaataaaga gttccagcag ctggatttct ccagtctgca tctttccgca ggcggtggga     5880

tgccagtgca gcaagtggtg gcagagcgtt gggtgaaact gaccggacag tatctgctgg     5940

aaggctatgg ccttaccgag tgtgcgccgc tggtcagcgt taacccatat gatattgatt     6000

atcatagtgg tagcatcggt ttgccggtgc cgtcgacgga agccaaactg gtggatgatg     6060

atgataatga agtaccacca ggtcaaccgg gtgagctttg tgtcaaagga ccgcaggtga     6120

tgctgggtta ctggcagcgt cccgatgcta ccgatgaaat catcaaaaat ggctggttac     6180

acaccggcga catcgcggta atggatgaag aaggattcct gcgcattgtc gatcgtaaaa     6240

aagacatgat tctggtttcc ggttttaacg tctatcccaa cgagattgaa gatgtcgtca     6300

tgcagcatcc tggcgtacag gaagtcgcgg ctgttggcgt accttccggc tccagtggtg     6360

aagcggtgaa aatcttcgta gtgaaaaaag atccatcgct taccgaagag tcactggtga     6420

ctttttgccg ccgtcagctc acgggataca aagtaccgaa gctggtggag tttcgtgatg     6480

agttaccgaa atctaacgtc ggaaaaattt tgcgacgaga attacgtgac gaagcgcgcg     6540

gcaaagtgga caataaagcc tga                                             6563


<210> 13
<211> 1686
<212> DNA
<213> Escherichia coli


<220> 
<223> FadD编码序列

<400> 13
ttgaagaagg tttggcttaa ccgttatccc gcggacgttc cgacggagat caaccctgac      60

cgttatcaat ctctggtaga tatgtttgag cagtcggtcg cgcgctacgc cgatcaacct     120

gcgtttgtga atatggggga ggtaatgacc ttccgcaagc tggaagaacg cagtcgcgcg     180

tttgccgctt atttgcaaca agggttgggg ctgaagaaag gcgatcgcgt tgcgttgatg     240

atgcctaatt tattgcaata tccggtggcg ctgtttggca ttttgcgtgc cgggatgatc     300

gtcgtaaacg ttaacccgtt gtataccccg cgtgagcttg agcatcagct taacgatagc     360

ggcgcatcgg cgattgttat cgtgtctaac tttgctcaca cactggaaaa agtggttgat     420

aaaaccgccg ttcagcacgt aattctgacc cgtatgggcg atcagctatc tacggcaaaa     480

ggcacggtag tcaatttcgt tgttaaatac atcaagcgtt tggtgccgaa ataccatctg     540

ccagatgcca tttcatttcg tagcgcactg cataacggct accggatgca gtacgtcaaa     600

cccgaactgg tgccggaaga tttagctttt ctgcaataca ccggcggcac cactggtgtg     660

gcgaaaggcg cgatgctgac tcaccgcaat atgctggcga acctggaaca ggttaacgcg     720

acctatggtc cgctgttgca tccgggcaaa gagctggtgg tgacggcgct gccgctgtat     780

cacatttttg ccctgaccat taactgcctg ctgtttatcg aactgggtgg gcagaacctg     840

cttatcacta acccgcgcga tattccaggg ttggtaaaag agttagcgaa atatccgttt     900

accgctatca cgggcgttaa caccttgttc aatgcgttgc tgaacaataa agagttccag     960

cagctggatt tctccagtct gcatctttcc gcaggcggtg ggatgccagt gcagcaagtg    1020

gtggcagagc gttgggtgaa actgaccgga cagtatctgc tggaaggcta tggccttacc    1080

gagtgtgcgc cgctggtcag cgttaaccca tatgatattg attatcatag tggtagcatc    1140

ggtttgccgg tgccgtcgac ggaagccaaa ctggtggatg atgatgataa tgaagtacca    1200

ccaggtcaac cgggtgagct ttgtgtcaaa ggaccgcagg tgatgctggg ttactggcag    1260

cgtcccgatg ctaccgatga aatcatcaaa aatggctggt tacacaccgg cgacatcgcg    1320

gtaatggatg aagaaggatt cctgcgcatt gtcgatcgta aaaaagacat gattctggtt    1380

tccggtttta acgtctatcc caacgagatt gaagatgtcg tcatgcagca tcctggcgta    1440

caggaagtcg cggctgttgg cgtaccttcc ggctccagtg gtgaagcggt gaaaatcttc    1500

gtagtgaaaa aagatccatc gcttaccgaa gagtcactgg tgactttttg ccgccgtcag    1560

ctcacgggat acaaagtacc gaagctggtg gagtttcgtg atgagttacc gaaatctaac    1620

gtcggaaaaa ttttgcgacg agaattacgt gacgaagcgc gcggcaaagt ggacaataaa    1680

gcctga                                                               1686


<210> 14
<211> 561
<212> PRT
<213> Escherichia coli


<220> 
<223> fadD氨基酸序列

<400> 14
Leu Lys Lys Val Trp Leu Asn Arg Tyr Pro Ala Asp Val Pro Thr Glu 
1               5                   10                  15      
Ile Asn Pro Asp Arg Tyr Gln Ser Leu Val Asp Met Phe Glu Gln Ser 
            20                  25                  30          
Val Ala Arg Tyr Ala Asp Gln Pro Ala Phe Val Asn Met Gly Glu Val 
        35                  40                  45              
Met Thr Phe Arg Lys Leu Glu Glu Arg Ser Arg Ala Phe Ala Ala Tyr 
    50                  55                  60                  
Leu Gln Gln Gly Leu Gly Leu Lys Lys Gly Asp Arg Val Ala Leu Met 
65                  70                  75                  80  
Met Pro Asn Leu Leu Gln Tyr Pro Val Ala Leu Phe Gly Ile Leu Arg 
                85                  90                  95      
Ala Gly Met Ile Val Val Asn Val Asn Pro Leu Tyr Thr Pro Arg Glu 
            100                 105                 110         
Leu Glu His Gln Leu Asn Asp Ser Gly Ala Ser Ala Ile Val Ile Val 
        115                 120                 125             
Ser Asn Phe Ala His Thr Leu Glu Lys Val Val Asp Lys Thr Ala Val 
    130                 135                 140                 
Gln His Val Ile Leu Thr Arg Met Gly Asp Gln Leu Ser Thr Ala Lys 
145                 150                 155                 160 
Gly Thr Val Val Asn Phe Val Val Lys Tyr Ile Lys Arg Leu Val Pro 
                165                 170                 175     
Lys Tyr His Leu Pro Asp Ala Ile Ser Phe Arg Ser Ala Leu His Asn 
            180                 185                 190         
Gly Tyr Arg Met Gln Tyr Val Lys Pro Glu Leu Val Pro Glu Asp Leu 
        195                 200                 205             
Ala Phe Leu Gln Tyr Thr Gly Gly Thr Thr Gly Val Ala Lys Gly Ala 
    210                 215                 220                 
Met Leu Thr His Arg Asn Met Leu Ala Asn Leu Glu Gln Val Asn Ala 
225                 230                 235                 240 
Thr Tyr Gly Pro Leu Leu His Pro Gly Lys Glu Leu Val Val Thr Ala 
                245                 250                 255     
Leu Pro Leu Tyr His Ile Phe Ala Leu Thr Ile Asn Cys Leu Leu Phe 
            260                 265                 270         
Ile Glu Leu Gly Gly Gln Asn Leu Leu Ile Thr Asn Pro Arg Asp Ile 
        275                 280                 285             
Pro Gly Leu Val Lys Glu Leu Ala Lys Tyr Pro Phe Thr Ala Ile Thr 
    290                 295                 300                 
Gly Val Asn Thr Leu Phe Asn Ala Leu Leu Asn Asn Lys Glu Phe Gln 
305                 310                 315                 320 
Gln Leu Asp Phe Ser Ser Leu His Leu Ser Ala Gly Gly Gly Met Pro 
                325                 330                 335     
Val Gln Gln Val Val Ala Glu Arg Trp Val Lys Leu Thr Gly Gln Tyr 
            340                 345                 350         
Leu Leu Glu Gly Tyr Gly Leu Thr Glu Cys Ala Pro Leu Val Ser Val 
        355                 360                 365             
Asn Pro Tyr Asp Ile Asp Tyr His Ser Gly Ser Ile Gly Leu Pro Val 
    370                 375                 380                 
Pro Ser Thr Glu Ala Lys Leu Val Asp Asp Asp Asp Asn Glu Val Pro 
385                 390                 395                 400 
Pro Gly Gln Pro Gly Glu Leu Cys Val Lys Gly Pro Gln Val Met Leu 
                405                 410                 415     
Gly Tyr Trp Gln Arg Pro Asp Ala Thr Asp Glu Ile Ile Lys Asn Gly 
            420                 425                 430         
Trp Leu His Thr Gly Asp Ile Ala Val Met Asp Glu Glu Gly Phe Leu 
        435                 440                 445             
Arg Ile Val Asp Arg Lys Lys Asp Met Ile Leu Val Ser Gly Phe Asn 
    450                 455                 460                 
Val Tyr Pro Asn Glu Ile Glu Asp Val Val Met Gln His Pro Gly Val 
465                 470                 475                 480 
Gln Glu Val Ala Ala Val Gly Val Pro Ser Gly Ser Ser Gly Glu Ala 
                485                 490                 495     
Val Lys Ile Phe Val Val Lys Lys Asp Pro Ser Leu Thr Glu Glu Ser 
            500                 505                 510         
Leu Val Thr Phe Cys Arg Arg Gln Leu Thr Gly Tyr Lys Val Pro Lys 
        515                 520                 525             
Leu Val Glu Phe Arg Asp Glu Leu Pro Lys Ser Asn Val Gly Lys Ile 
    530                 535                 540                 
Leu Arg Arg Glu Leu Arg Asp Glu Ala Arg Gly Lys Val Asp Asn Lys 
545                 550                 555                 560 
Ala 
    

<210> 15
<211> 6049
<212> DNA
<213> Artificial Sequence


<220> 
<223> p15A-Prha-OLS

<400> 15
agcggataac aatttcacac aggagagctc aaagagtgga acaatgcagg acgccgtccg       60

gtaacggtgt tgagcctcgg ttgtgtggtg attattgtcg ccgctaacat cgtcggcatc      120

ggcatggcga attaatcttt ctgcgaattg agatgacgcc actggctggg cgtcatcccg      180

gtttcccggg taaacaccac cgaaaaatag ttactatctt caaagccaca ttcggtcgaa      240

atatcactga ttaacaggcg gctatgctgg agaagatatt gcgcatgaca cactctgacc      300

tgtcgcagat attgattgat ggtcattcca gtctgctggc gaaattgctg acgcaaaacg      360

cgctcactgc acgatgcctc atcacaaaat ttatccagcg caaagggact tttcaggcta      420

gccgccagcc gggtaatcag cttatccagc aacgtttcgc tggatgttgg cggcaacgaa      480

tcactggtgt aacgatggcg attcagcaac atcaccaact gcccgaacag caactcagcc      540

atttcgttag caaacggcac atgctgacta ctttcatgct caagctgacc gataacctgc      600

cgcgcctgcg ccatccccat gctacctaag cgccagtgtg gttgccctgc gctggcgtta      660

aatcccggaa tcgccccctg ccagtcaaga ttcagcttca gacgctccgg gcaataaata      720

atattctgca aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc agcatgaatg      780

taaaagagat cgccacgggt aatgcgataa gggcgatcgt tgagtacatg caggccatta      840

ccgcgccaga caatcaccag ctcacaaaaa tcatgtgtat gttcagcaaa gacatcttgc      900

ggataacggt cagccacagc gactgcctgc tggtcgctgg caaaaaaatc atctttgaga      960

agttttaact gatgcgccac cgtggctacc tcggccagag aacgaagttg attattcgca     1020

atatggcgta caaatacgtt gagaagattc gcgttattgc agaaagccat cccgtccctg     1080

gcgaatatca cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa agtggttact     1140

gtcgctgaat ccacagcgat aggcgatgtc agtaacgctg gcctcgctgt ggcgtagcag     1200

atgtcgggct ttcatcagtc gcaggcggtt caggtatcgc tgaggcgtca gtcccgtttg     1260

ctgcttaagc tgccgatgta gcgtacgcag tgaaagagaa aattgatccg ccacggcatc     1320

ccaattcacc tcatcggcaa aatggtcctc cagccaggcc agaagcaagt tgagacgtga     1380

tgcgctgttt tccaggttct cctgcaaact gcttttacgc agcaagagca gtaattgcat     1440

aaacaagatc tcgcgactgg cggtcgaggg taaatcattt tccccttcct gctgttccat     1500

ctgtgcaacc agctgtcgca cctgctgcaa tacgctgtgg ttaacgcgcc agtgagacgg     1560

atactgccca tccagctctt gtggcagcaa ctgattcagc ccggcgagaa actgaaatcg     1620

atccggcgag cgatacagca cattggtcag acacagatta tcggtatgtt catacagatg     1680

ccgatcatga tcgcgtacga aacagaccgt gccaccggtg atggtatagg gctgcccatt     1740

aaacacatga atacccgtgc catgttcgac aatcacaatt tcatgaaaat catgatgatg     1800

ttcaggaaaa tccgcctgcg ggagccgggg ttctatcgcc acggacgcgt taccagacgg     1860

aaaaaaatcc acactatgta atacggtcat actggcctcc tgatgtcgtc aacacggcga     1920

aatagtaatc acgaggtcag gttcttacct taaattttcg acggaaaacc acgtaaaaaa     1980

cgtcgatttt tcaagataca gcgtgaattt tcaggaaatg cggtgagcat cacatcacca     2040

caattcagca aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt     2100

ttcctgtcag taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa     2160

ttcaactagt gctctgcagg agctgtcacc ggatgtgctt tccggtctga tgagtccgtg     2220

aggacgaaac agcctctaca aataattttg tttaagagtt actagagagg aggaattaac     2280

catgaaccat ctgcgtgcgg aaggccctgc gagcgtttta gcgattggca ccgcgaatcc     2340

ggaaaacatt ctgctgcagg atgaatttcc ggattattat tttcgcgtga ccaaaagcga     2400

acatatgacc cagctgaaag aaaaatttcg caaaatttgc gacaagagca tgattcgcaa     2460

acgcaactgc tttctgaacg aagaacatct gaaacagaac ccgcgcctgg tggaacatga     2520

aatgcagacc ctggatgcgc gccaggatat gctggtggtg gaagtgccga aactgggcaa     2580

agatgcgtgc gcgaaagcga ttaaagaatg gggccagccg aaaagcaaaa ttacccatct     2640

gatttttacc agcgcgagca ccaccgatat gccgggcgca gattatcatt gcgcgaaact     2700

gctgggcctg agcccgagcg ttaaacgcgt gatgatgtat cagctgggct gctatggcgg     2760

cggcaccgtt ttacgtattg cgaaagatat tgcggaaaac aacaaaggcg cgcgcgtgct     2820

ggcggtgtgt tgtgatatta tggcgtgcct gtttcgcggc ccgagcgaaa gcgatctgga     2880

actgttagtg ggccaggcga tttttggcga tggcgcggcg gcggtgattg tgggtgcaga     2940

acctgatgaa agcgtgggcg aacgccctat ttttgaactg gtgagcaccg gccagaccat     3000

tctgccgaat agcgaaggca ccattggcgg ccatattcgc gaagcgggcc tgatttttga     3060

tctgcataaa gatgtgccga tgctgattag caacaacatt gaaaaatgcc tgattgaggc     3120

gtttaccccg attggcatta gcgattggaa cagcatcttt tggattaccc atccgggcgg     3180

caaagcgatt ctggataaag tggaagaaaa actgcatctg aaaagcgata aattcgtgga     3240

tagccgccat gtgctgagcg aacatggcaa catgagcagc agcaccgtgc tgtttgtgat     3300

ggatgaactg cgcaaacgca gcctggaaga aggcaaaagc accaccggcg atggctttga     3360

atggggcgtg ctgtttggct ttggcccggg cttaaccgtg gaacgcgttg tggttcgtag     3420

cgtgcctatt aaatattaac tcgtcgtgac tgggaaaacc ctggcgacta gtcttggact     3480

cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg     3540

ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt acgatttaaa     3600

ttggcgaaaa tgagacgtgg gtctgacgct cagtggaacg aaaactcacg ttaagggatt     3660

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttggttca tgtgcagctc     3720

catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag cgccggtgat     3780

cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc     3840

gaaaagccga gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc     3900

tggtccacag ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg     3960

aggccctgcg tgctgcgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc     4020

tggacgacga gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct     4080

ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg     4140

cagcggggcc acaggcagag cagatcatct ctgatccatt gcccctgcca cctcactcgc     4200

ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg     4260

gacacgatgc caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg     4320

tgccgagaca ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga     4380

atgaccactg ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc     4440

agaaggaagg tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg     4500

cgacagccct gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg     4560

gatgcgaaga atgcgatgcc gctcgccagt cgattggctg agctcatgag cggagaacga     4620

gatgacgttg gaggggcaag gtcgcgctga ttgctggggc aacacgtgga gcggatcggt     4680

ttgacttttg tccttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc     4740

ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt     4800

tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag     4860

cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc     4920

tctgcgaggc tggccgtagg ccggccgcga tgcaggtggc tgctgaaccc ccagccggaa     4980

ctgaccccac aaggccctag cggagtgtat actggcttac tatgttggca ctgatgaggg     5040

tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat     5100

atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga     5160

ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag     5220

atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc     5280

ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat     5340

aaagatacca ggcgtttccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg     5400

gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca     5460

gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg     5520

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag     5580

caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc     5640

ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct     5700

cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt     5760

tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt     5820

aactacatgg ctctgctgta gtgagtgggt tgcgctccgg cagcggtcct gatcccccgc     5880

agaaaaaaag gatctcaaga agatcctttg atcttttcta cggcgcgccc agctgtctag     5940

ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag     6000

gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tttaattaa                 6049


