﻿                         序列表

<110>  山西锦波生物医药股份有限公司

<120>  一种重组I型人源化胶原蛋白多肽及其制备方法和用途

<130>  6C39-2183148IB

<150>  202110968550.2
<151>  2021-08-23

<160>  18

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  60
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  重复序列单元

<400>  1
Gly Glu Lys Gly Ser Pro Gly Ala Asp Gly Pro Ala Gly Ala Pro Gly 
1               5                   10                  15      
Thr Pro Gly Pro Gln Gly Ile Ala Gly Gln Arg Gly Val Val Gly Leu 
            20                  25                  30          
Pro Gly Gln Arg Gly Glu Arg Gly Phe Pro Gly Leu Pro Gly Pro Ser 
        35                  40                  45              
Gly Glu Pro Gly Lys Gln Gly Pro Ser Gly Ala Ser 
    50                  55                  60  

<210>  2
<211>  10
<212>  PRT
<213>  人工序列(Artificial Sequence)
<220>
<223>  铰链区氨基酸序列

<400>  2
Gly Pro Pro Gly Pro Cys Cys Gly Gly Gly 
1               5                   10  

<210>  3
<211>  240
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  TC1R4的氨基酸序列

<400>  3
Gly Glu Lys Gly Ser Pro Gly Ala Asp Gly Pro Ala Gly Ala Pro Gly 
1               5                   10                  15      
Thr Pro Gly Pro Gln Gly Ile Ala Gly Gln Arg Gly Val Val Gly Leu 
            20                  25                  30          
Pro Gly Gln Arg Gly Glu Arg Gly Phe Pro Gly Leu Pro Gly Pro Ser 
        35                  40                  45              
Gly Glu Pro Gly Lys Gln Gly Pro Ser Gly Ala Ser Gly Glu Lys Gly 
    50                  55                  60                  
Ser Pro Gly Ala Asp Gly Pro Ala Gly Ala Pro Gly Thr Pro Gly Pro 
65                  70                  75                  80  
Gln Gly Ile Ala Gly Gln Arg Gly Val Val Gly Leu Pro Gly Gln Arg 
                85                  90                  95      
Gly Glu Arg Gly Phe Pro Gly Leu Pro Gly Pro Ser Gly Glu Pro Gly 
            100                 105                 110         
Lys Gln Gly Pro Ser Gly Ala Ser Gly Glu Lys Gly Ser Pro Gly Ala 
        115                 120                 125             
Asp Gly Pro Ala Gly Ala Pro Gly Thr Pro Gly Pro Gln Gly Ile Ala 
    130                 135                 140                 
Gly Gln Arg Gly Val Val Gly Leu Pro Gly Gln Arg Gly Glu Arg Gly 
145                 150                 155                 160 
Phe Pro Gly Leu Pro Gly Pro Ser Gly Glu Pro Gly Lys Gln Gly Pro 
                165                 170                 175     
Ser Gly Ala Ser Gly Glu Lys Gly Ser Pro Gly Ala Asp Gly Pro Ala 
            180                 185                 190         
Gly Ala Pro Gly Thr Pro Gly Pro Gln Gly Ile Ala Gly Gln Arg Gly 
        195                 200                 205             
Val Val Gly Leu Pro Gly Gln Arg Gly Glu Arg Gly Phe Pro Gly Leu 
    210                 215                 220                 
Pro Gly Pro Ser Gly Glu Pro Gly Lys Gln Gly Pro Ser Gly Ala Ser 
225                 230                 235                 240 

<210>  4
<211>  6
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  可被TEV蛋白酶切除的序列

<400>  4
Glu Asn Leu Tyr Phe Gln 
1               5       

<210>  5
<211>  246
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  具有可被TEV蛋白酶切除的序列的TC1R4的氨基酸序列

<400>  5
Glu Asn Leu Tyr Phe Gln Gly Glu Lys Gly Ser Pro Gly Ala Asp Gly 
1               5                   10                  15      
Pro Ala Gly Ala Pro Gly Thr Pro Gly Pro Gln Gly Ile Ala Gly Gln 
            20                  25                  30          
Arg Gly Val Val Gly Leu Pro Gly Gln Arg Gly Glu Arg Gly Phe Pro 
        35                  40                  45              
Gly Leu Pro Gly Pro Ser Gly Glu Pro Gly Lys Gln Gly Pro Ser Gly 
    50                  55                  60                  
Ala Ser Gly Glu Lys Gly Ser Pro Gly Ala Asp Gly Pro Ala Gly Ala 
65                  70                  75                  80  
Pro Gly Thr Pro Gly Pro Gln Gly Ile Ala Gly Gln Arg Gly Val Val 
                85                  90                  95      
Gly Leu Pro Gly Gln Arg Gly Glu Arg Gly Phe Pro Gly Leu Pro Gly 
            100                 105                 110         
Pro Ser Gly Glu Pro Gly Lys Gln Gly Pro Ser Gly Ala Ser Gly Glu 
        115                 120                 125             
Lys Gly Ser Pro Gly Ala Asp Gly Pro Ala Gly Ala Pro Gly Thr Pro 
    130                 135                 140                 
Gly Pro Gln Gly Ile Ala Gly Gln Arg Gly Val Val Gly Leu Pro Gly 
145                 150                 155                 160 
Gln Arg Gly Glu Arg Gly Phe Pro Gly Leu Pro Gly Pro Ser Gly Glu 
                165                 170                 175     
Pro Gly Lys Gln Gly Pro Ser Gly Ala Ser Gly Glu Lys Gly Ser Pro 
            180                 185                 190         
Gly Ala Asp Gly Pro Ala Gly Ala Pro Gly Thr Pro Gly Pro Gln Gly 
        195                 200                 205             
Ile Ala Gly Gln Arg Gly Val Val Gly Leu Pro Gly Gln Arg Gly Glu 
    210                 215                 220                 
Arg Gly Phe Pro Gly Leu Pro Gly Pro Ser Gly Glu Pro Gly Lys Gln 
225                 230                 235                 240 
Gly Pro Ser Gly Ala Ser 
                245     

<210>  6
<211>  753
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  包含酶切位点、终止密码子和可被TEV蛋白酶切除的序列的TC1R4的DNA序列

<400>  6
ggtaccgaaa acctgtattt ccagggtgaa aaaggtagtc cgggtgcaga tggtccggcc        60
ggtgcacctg gtacaccggg tcctcagggc attgcaggcc agcgtggtgt tgtgggcctg       120
ccgggtcagc gcggtgaacg tggttttccg ggtctgccgg gtccgagcgg cgaacctggt       180
aaacagggcc cgagtggtgc aagcggtgaa aaaggcagtc cgggcgcaga tggtcctgca       240
ggtgcccctg gtacacctgg tccgcagggt attgcaggtc agcgcggcgt ggtgggcctg       300
cctggtcaac gtggcgaacg tggtttcccg ggcctgccgg gaccgtcagg tgaacctggc       360
aaacagggcc ctagcggtgc aagtggcgaa aaaggtagcc cgggcgcaga cggcccggca       420
ggtgcacctg gcacacctgg tcctcagggt attgcgggcc agcgcggcgt tgttggtctg       480
cctggccagc gtggcgaacg cggttttccg ggcctgcctg gcccgtcagg cgaacctgga       540
aaacagggcc caagtggtgc aagtggtgaa aaaggaagtc cgggcgccga tggcccggcc       600
ggtgcgcctg gtacgcctgg tcctcaaggc attgccggtc agcgcggagt tgttggcctg       660
ccgggccagc gtggagaacg cggtttcccg ggtctgcctg gtccgagtgg tgaaccgggt       720
aaacagggtc cgagcggtgc atcataactc gag                                    753

<210>  7
<211>  18
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  TC1R4质谱检测结果部分序列

<400>  7
Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro 
1               5                   10                  15      
Cys Lys 
        

<210>  8
<211>  27
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  TC1R4质谱检测结果部分序列

<400>  8
Gly Glu Arg Gly Phe Pro Gly Leu Pro Gly Pro Ser Gly Glu Pro Gly 
1               5                   10                  15      
Lys Gln Gly Pro Ser Gly Ala Ser Gly Glu Lys 
            20                  25          

<210>  9
<211>  24
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  TC1R4质谱检测结果部分序列

<400>  9
Gly Phe Pro Gly Leu Pro Gly Pro Ser Gly Glu Pro Gly Lys Gln Gly 
1               5                   10                  15      
Pro Ser Gly Ala Ser Gly Glu Lys 
            20                  

<210>  10
<211>  51
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  TC1R4质谱检测结果部分序列

<400>  10
Gly Glu Arg Gly Phe Pro Gly Leu Pro Gly Pro Ser Gly Glu Pro Gly 
1               5                   10                  15      
Lys Gln Gly Pro Ser Gly Ala Ser Gly Glu Lys Gly Ser Pro Gly Ala 
            20                  25                  30          
Asp Gly Pro Ala Gly Ala Pro Gly Thr Pro Gly Pro Gln Gly Ile Ala 
        35                  40                  45              
Gly Gln Arg 
    50      

<210>  11
<211>  34
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  TC1R4质谱检测结果部分序列

<400>  11
Gln Gly Pro Ser Gly Ala Ser Gly Glu Lys Gly Ser Pro Gly Ala Asp 
1               5                   10                  15      
Gly Pro Ala Gly Ala Pro Gly Thr Pro Gly Pro Gln Gly Ile Ala Gly 
            20                  25                  30          
Gln Arg 
        

<210>  12
<211>  24
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  TC1R4质谱检测结果部分序列

<400>  12
Gly Ser Pro Gly Ala Asp Gly Pro Ala Gly Ala Pro Gly Thr Pro Gly 
1               5                   10                  15      
Pro Gln Gly Ile Ala Gly Gln Arg 
            20                  

<210>  13
<211>  9
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  TC1R4质谱检测结果部分序列

<400>  13
Gly Val Val Gly Leu Pro Gly Gln Arg 
1               5                   

<210>  14
<211>  12
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  TC1R4质谱检测结果部分序列

<400>  14
Gly Val Val Gly Leu Pro Gly Gln Arg Gly Glu Arg 
1               5                   10          

<210>  15
<211>  17
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  TC1R4质谱检测结果部分序列

<400>  15
Gly Glu Arg Gly Phe Pro Gly Leu Pro Gly Pro Ser Gly Glu Pro Gly 
1               5                   10                  15      
Lys 
    

<210>  16
<211>  24
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  TC1R4质谱检测结果部分序列

<400>  16
Gly Glu Arg Gly Phe Pro Gly Leu Pro Gly Pro Ser Gly Glu Pro Gly 
1               5                   10                  15      
Lys Gln Gly Pro Ser Gly Ala Ser 
            20                  

<210>  17
<211>  14
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  TC1R4质谱检测结果部分序列

<400>  17
Gly Phe Pro Gly Leu Pro Gly Pro Ser Gly Glu Pro Gly Lys 
1               5                   10                  

<210>  18
<211>  21
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  TC1R4质谱检测结果部分序列

<400>  18
Gly Phe Pro Gly Leu Pro Gly Pro Ser Gly Glu Pro Gly Lys Gln Gly 
1               5                   10                  15      
Pro Ser Gly Ala Ser 
            20      

