               SEQUENCE LISTING

<110> Intervet International B.V.

<120> FMDV Virus-like Particle with Stabilizing Mutation

<130> 25231

<160> 6

<170> BiSSAP 1.3.6

<210> 1
<211> 214
<212> PRT
<213> Foot-and-mouth disease virus - type SAT 2


<220> 
<223> SAT2/SAU/6/2000 GenBank: AY297948.1	VP1 wild type

<400> 1
Thr Thr Ser Ala Gly Glu Ser Ala Asp Val Val Thr Thr Asp Pro Ser 
1               5                   10                  15      
Thr His Gly Gly Asn Val Gln Glu Gly Arg Arg Lys His Thr Glu Val 
            20                  25                  30          
Ala Phe Leu Leu Asp Arg Ser Thr His Val His Thr Asn Lys Thr Ser 
        35                  40                  45              
Phe Val Val Asp Leu Met Asp Thr Lys Glu Lys Ala Leu Val Gly Ala 
    50                  55                  60                  
Ile Leu Arg Ala Ser Thr Tyr Tyr Phe Cys Asp Leu Glu Ile Ala Cys 
65                  70                  75                  80  
Val Gly Asp His Thr Arg Ala Phe Trp Gln Pro Asn Gly Ala Pro Arg 
                85                  90                  95      
Thr Thr Gln Leu Gly Asp Asn Pro Met Val Phe Ala Lys Gly Gly Val 
            100                 105                 110         
Thr Arg Phe Ala Ile Pro Phe Thr Ala Pro His Arg Leu Leu Ser Thr 
        115                 120                 125             
Val Tyr Asn Gly Glu Cys Val Tyr Lys Lys Thr Pro Thr Ala Ile Arg 
    130                 135                 140                 
Gly Asp Arg Ala Ala Leu Ala Val Lys Tyr Ala Asp Ser Thr His Thr 
145                 150                 155                 160 
Leu Pro Ser Thr Phe Asn Phe Gly Phe Val Thr Val Asp Lys Pro Val 
                165                 170                 175     
Asp Val Tyr Tyr Arg Met Lys Arg Ala Glu Leu Tyr Cys Pro Arg Pro 
            180                 185                 190         
Leu Leu Pro Ala Tyr Glu His Thr Gly Gly Asp Arg Phe Asp Ala Pro 
        195                 200                 205             
Ile Gly Val Glu Arg Gln 
    210                 

<210> 2
<211> 85
<212> PRT
<213> Foot-and-mouth disease virus - type SAT 2


<220> 
<223> SAT2/SAU/6/2000 GenBank: AY297948.1	VP4 wild type

<400> 2
Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asp Gln Ser Gly 
1               5                   10                  15      
Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 
            20                  25                  30          
Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 
        35                  40                  45              
Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Asn Asn Thr Gln Asn 
    50                  55                  60                  
Asn Asp Trp Phe Ser Lys Leu Ala Gln Ser Ala Ile Ser Gly Leu Phe 
65                  70                  75                  80  
Gly Ala Leu Leu Ala 
                85  

<210> 3
<211> 713
<212> PRT
<213> Foot-and-mouth disease virus - type SAT 2


<220> 
<223> SAT2/SAU/6/2000 GenBank: AY297948.1	P1 wild type

<400> 3
Met Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asp Gln Ser 
1               5                   10                  15      
Gly Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln 
            20                  25                  30          
Asn Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser 
        35                  40                  45              
Asn Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Asn Asn Thr Gln 
    50                  55                  60                  
Asn Asn Asp Trp Phe Ser Lys Leu Ala Gln Ser Ala Ile Ser Gly Leu 
65                  70                  75                  80  
Phe Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu 
                85                  90                  95      
Glu Asp Arg Ile Leu Thr Thr Arg His Gly Thr Thr Thr Ser Thr Thr 
            100                 105                 110         
Gln Ser Ser Val Gly Val Thr Leu Gly Tyr Ala Asp Ala Asp Ser Phe 
        115                 120                 125             
Arg Pro Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Gln Gln Ala 
    130                 135                 140                 
Glu Arg Phe Phe Lys Glu Lys Leu Phe Asp Trp Thr Ser Asp Lys Pro 
145                 150                 155                 160 
Phe Gly Thr Leu Tyr Val Leu Glu Leu Pro Lys Asp His Lys Gly Ile 
                165                 170                 175     
Tyr Gly Lys Leu Thr Asp Ser Tyr Thr Tyr Met Arg Asn Gly Trp Asp 
            180                 185                 190         
Val Gln Val Ser Ala Thr Ser Thr Gln Phe Asn Gly Gly Ser Leu Leu 
        195                 200                 205             
Val Ala Met Val Pro Glu Leu Ser Ser Leu Lys Ser Arg Glu Glu Phe 
    210                 215                 220                 
Gln Leu Thr Leu Tyr Pro His Gln Phe Ile Asn Pro Arg Thr Asn Thr 
225                 230                 235                 240 
Thr Ala His Ile Gln Val Pro Tyr Leu Gly Val Asn Arg His Asp Gln 
                245                 250                 255     
Gly Lys Arg His His Ala Trp Ser Leu Val Val Met Val Leu Thr Pro 
            260                 265                 270         
Leu Thr Thr Glu Ala Gln Met Asn Ser Gly Thr Val Glu Val Tyr Ala 
        275                 280                 285             
Asn Ile Ala Pro Thr Asn Val Val Val Ala Gly Glu Leu Pro Gly Lys 
    290                 295                 300                 
Gln Gly Ile Val Pro Val Ala Ala Ala Asp Gly Tyr Gly Gly Phe Gln 
305                 310                 315                 320 
Asn Thr Asp Pro Lys Thr Ala Asp Pro Ile Tyr Gly Tyr Val Tyr Asn 
                325                 330                 335     
Pro Ser Arg Asn Asp Cys His Gly Arg Phe Ser Asn Leu Leu Asp Val 
            340                 345                 350         
Ala Glu Ala Cys Pro Thr Leu Leu Asp Phe Asp Gly Lys Pro Tyr Ile 
        355                 360                 365             
Val Thr Lys Asn Asn Gly Asp Lys Val Met Thr Ser Phe Asp Val Ala 
    370                 375                 380                 
Phe Thr His Lys Val His Arg Asn Thr Phe Leu Ala Gly Leu Ala Asp 
385                 390                 395                 400 
Tyr Tyr Thr Gln Tyr Ser Gly Ser Leu Asn Tyr His Phe Met Tyr Thr 
                405                 410                 415     
Gly Pro Thr His His Lys Ala Lys Phe Met Val Ala Tyr Val Pro Pro 
            420                 425                 430         
Gly Val Glu Thr Ala Gln Leu Pro Thr Thr Pro Glu Asp Ala Ala His 
        435                 440                 445             
Cys Tyr His Ala Glu Trp Asp Thr Gly Leu Asn Ser Ser Phe Ser Phe 
    450                 455                 460                 
Ala Val Pro Tyr Ile Ser Ala Ala Asp Phe Ser Tyr Thr His Thr Asp 
465                 470                 475                 480 
Thr Pro Ala Met Ala Thr Thr Asn Gly Trp Val Ile Val Leu Gln Val 
                485                 490                 495     
Thr Asp Thr His Ser Ala Glu Ala Ala Val Val Val Ser Val Ser Ala 
            500                 505                 510         
Gly Pro Asp Leu Glu Phe Arg Phe Pro Ile Asp Pro Val Arg Gln Thr 
        515                 520                 525             
Thr Ser Ala Gly Glu Ser Ala Asp Val Val Thr Thr Asp Pro Ser Thr 
    530                 535                 540                 
His Gly Gly Asn Val Gln Glu Gly Arg Arg Lys His Thr Glu Val Ala 
545                 550                 555                 560 
Phe Leu Leu Asp Arg Ser Thr His Val His Thr Asn Lys Thr Ser Phe 
                565                 570                 575     
Val Val Asp Leu Met Asp Thr Lys Glu Lys Ala Leu Val Gly Ala Ile 
            580                 585                 590         
Leu Arg Ala Ser Thr Tyr Tyr Phe Cys Asp Leu Glu Ile Ala Cys Val 
        595                 600                 605             
Gly Asp His Thr Arg Ala Phe Trp Gln Pro Asn Gly Ala Pro Arg Thr 
    610                 615                 620                 
Thr Gln Leu Gly Asp Asn Pro Met Val Phe Ala Lys Gly Gly Val Thr 
625                 630                 635                 640 
Arg Phe Ala Ile Pro Phe Thr Ala Pro His Arg Leu Leu Ser Thr Val 
                645                 650                 655     
Tyr Asn Gly Glu Cys Val Tyr Lys Lys Thr Pro Thr Ala Ile Arg Gly 
            660                 665                 670         
Asp Arg Ala Ala Leu Ala Val Lys Tyr Ala Asp Ser Thr His Thr Leu 
        675                 680                 685             
Pro Ser Thr Phe Asn Phe Gly Phe Val Thr Val Asp Lys Pro Val Asp 
    690                 695                 700                 
Val Tyr Tyr Arg Met Lys Arg Ala Glu 
705                 710             

<210> 4
<211> 214
<212> PRT
<213> Foot-and-mouth disease virus - type SAT 2


<220> 
<223> SAT2/SAU/6/2000 GenBank: AY297948.1	VP1 mutant

<400> 4
Thr Thr Ser Ala Gly Glu Ser Ala Asp Val Val Asn Thr Asp Pro Ser 
1               5                   10                  15      
Thr His Gly Gly Asn Val Gln Glu Gly Arg Arg Lys His Thr Glu Val 
            20                  25                  30          
Ala Phe Leu Leu Asp Arg Ser Thr His Val His Thr Asn Lys Thr Ser 
        35                  40                  45              
Phe Val Val Asp Leu Met Asp Thr Lys Glu Lys Ala Leu Val Gly Ala 
    50                  55                  60                  
Ile Leu Arg Ala Ser Thr Tyr Tyr Phe Cys Asp Leu Glu Ile Ala Cys 
65                  70                  75                  80  
Val Gly Asp His Thr Arg Ala Phe Trp Gln Pro Asn Gly Ala Pro Arg 
                85                  90                  95      
Thr Thr Gln Leu Gly Asp Asn Pro Met Val Phe Ala Lys Gly Gly Val 
            100                 105                 110         
Thr Arg Phe Ala Ile Pro Phe Thr Ala Pro His Arg Leu Leu Ser Thr 
        115                 120                 125             
Val Tyr Asn Gly Glu Cys Val Tyr Lys Lys Thr Pro Thr Ala Ile Arg 
    130                 135                 140                 
Gly Asp Arg Ala Ala Leu Ala Val Lys Tyr Ala Asp Ser Thr His Thr 
145                 150                 155                 160 
Leu Pro Ser Thr Phe Asn Phe Gly Phe Val Thr Val Asp Lys Pro Val 
                165                 170                 175     
Asp Val Tyr Tyr Arg Met Lys Arg Ala Glu Leu Tyr Cys Pro Arg Pro 
            180                 185                 190         
Leu Leu Pro Ala Tyr Glu His Thr Gly Gly Asp Arg Phe Asp Ala Pro 
        195                 200                 205             
Ile Gly Val Glu Arg Gln 
    210                 

<210> 5
<211> 85
<212> PRT
<213> Foot-and-mouth disease virus - type SAT 2


<220> 
<223> SAT2/SAU/6/2000 GenBank: AY297948.1	VP4 modified

<400> 5
Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asp Gln Ser Gly 
1               5                   10                  15      
Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 
            20                  25                  30          
Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 
        35                  40                  45              
Glu Gly Ser Thr Gly Thr Thr Ser Thr His Thr Asn Asn Thr Gln Asn 
    50                  55                  60                  
Asn Asp Trp Phe Ser Lys Leu Ala Gln Ser Ala Ile Ser Gly Leu Phe 
65                  70                  75                  80  
Gly Ala Leu Leu Ala 
                85  

<210> 6
<211> 741
<212> PRT
<213> Foot-and-mouth disease virus - type SAT 2


<220> 
<223> SAT2/SAU/6/2000 GenBank: AY297948.1	P1 double mutant

<400> 6
Met Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asp Gln Ser 
1               5                   10                  15      
Gly Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln 
            20                  25                  30          
Asn Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser 
        35                  40                  45              
Asn Glu Gly Ser Thr Gly Thr Thr Ser Thr His Thr Asn Asn Thr Gln 
    50                  55                  60                  
Asn Asn Asp Trp Phe Ser Lys Leu Ala Gln Ser Ala Ile Ser Gly Leu 
65                  70                  75                  80  
Phe Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu 
                85                  90                  95      
Glu Asp Arg Ile Leu Thr Thr Arg His Gly Thr Thr Thr Ser Thr Thr 
            100                 105                 110         
Gln Ser Ser Val Gly Val Thr Leu Gly Tyr Ala Asp Ala Asp Ser Phe 
        115                 120                 125             
Arg Pro Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Gln Gln Ala 
    130                 135                 140                 
Glu Arg Phe Phe Lys Glu Lys Leu Phe Asp Trp Thr Ser Asp Lys Pro 
145                 150                 155                 160 
Phe Gly Thr Leu Tyr Val Leu Glu Leu Pro Lys Asp His Lys Gly Ile 
                165                 170                 175     
Tyr Gly Lys Leu Thr Asp Ser Tyr Thr Tyr Met Arg Asn Gly Trp Asp 
            180                 185                 190         
Val Gln Val Ser Ala Thr Ser Thr Gln Phe Asn Gly Gly Ser Leu Leu 
        195                 200                 205             
Val Ala Met Val Pro Glu Leu Ser Ser Leu Lys Ser Arg Glu Glu Phe 
    210                 215                 220                 
Gln Leu Thr Leu Tyr Pro His Gln Phe Ile Asn Pro Arg Thr Asn Thr 
225                 230                 235                 240 
Thr Ala His Ile Gln Val Pro Tyr Leu Gly Val Asn Arg His Asp Gln 
                245                 250                 255     
Gly Lys Arg His His Ala Trp Ser Leu Val Val Met Val Leu Thr Pro 
            260                 265                 270         
Leu Thr Thr Glu Ala Gln Met Asn Ser Gly Thr Val Glu Val Tyr Ala 
        275                 280                 285             
Asn Ile Ala Pro Thr Asn Val Val Val Ala Gly Glu Leu Pro Gly Lys 
    290                 295                 300                 
Gln Gly Ile Val Pro Val Ala Ala Ala Asp Gly Tyr Gly Gly Phe Gln 
305                 310                 315                 320 
Asn Thr Asp Pro Lys Thr Ala Asp Pro Ile Tyr Gly Tyr Val Tyr Asn 
                325                 330                 335     
Pro Ser Arg Asn Asp Cys His Gly Arg Phe Ser Asn Leu Leu Asp Val 
            340                 345                 350         
Ala Glu Ala Cys Pro Thr Leu Leu Asp Phe Asp Gly Lys Pro Tyr Ile 
        355                 360                 365             
Val Thr Lys Asn Asn Gly Asp Lys Val Met Thr Ser Phe Asp Val Ala 
    370                 375                 380                 
Phe Thr His Lys Val His Arg Asn Thr Phe Leu Ala Gly Leu Ala Asp 
385                 390                 395                 400 
Tyr Tyr Thr Gln Tyr Ser Gly Ser Leu Asn Tyr His Phe Met Tyr Thr 
                405                 410                 415     
Gly Pro Thr His His Lys Ala Lys Phe Met Val Ala Tyr Val Pro Pro 
            420                 425                 430         
Gly Val Glu Thr Ala Gln Leu Pro Thr Thr Pro Glu Asp Ala Ala His 
        435                 440                 445             
Cys Tyr His Ala Glu Trp Asp Thr Gly Leu Asn Ser Ser Phe Ser Phe 
    450                 455                 460                 
Ala Val Pro Tyr Ile Ser Ala Ala Asp Phe Ser Tyr Thr His Thr Asp 
465                 470                 475                 480 
Thr Pro Ala Met Ala Thr Thr Asn Gly Trp Val Ile Val Leu Gln Val 
                485                 490                 495     
Thr Asp Thr His Ser Ala Glu Ala Ala Val Val Val Ser Val Ser Ala 
            500                 505                 510         
Gly Pro Asp Leu Glu Phe Arg Phe Pro Ile Asp Pro Val Arg Gln Thr 
        515                 520                 525             
Thr Ser Ala Gly Glu Ser Ala Asp Val Val Asn Thr Asp Pro Ser Thr 
    530                 535                 540                 
His Gly Gly Asn Val Gln Glu Gly Arg Arg Lys His Thr Glu Val Ala 
545                 550                 555                 560 
Phe Leu Leu Asp Arg Ser Thr His Val His Thr Asn Lys Thr Ser Phe 
                565                 570                 575     
Val Val Asp Leu Met Asp Thr Lys Glu Lys Ala Leu Val Gly Ala Ile 
            580                 585                 590         
Leu Arg Ala Ser Thr Tyr Tyr Phe Cys Asp Leu Glu Ile Ala Cys Val 
        595                 600                 605             
Gly Asp His Thr Arg Ala Phe Trp Gln Pro Asn Gly Ala Pro Arg Thr 
    610                 615                 620                 
Thr Gln Leu Gly Asp Asn Pro Met Val Phe Ala Lys Gly Gly Val Thr 
625                 630                 635                 640 
Arg Phe Ala Ile Pro Phe Thr Ala Pro His Arg Leu Leu Ser Thr Val 
                645                 650                 655     
Tyr Asn Gly Glu Cys Val Tyr Lys Lys Thr Pro Thr Ala Ile Arg Gly 
            660                 665                 670         
Asp Arg Ala Ala Leu Ala Val Lys Tyr Ala Asp Ser Thr His Thr Leu 
        675                 680                 685             
Pro Ser Thr Phe Asn Phe Gly Phe Val Thr Val Asp Lys Pro Val Asp 
    690                 695                 700                 
Val Tyr Tyr Arg Met Lys Arg Ala Glu Leu Tyr Cys Pro Arg Pro Leu 
705                 710                 715                 720 
Leu Pro Ala Tyr Glu His Thr Gly Gly Asp Arg Phe Asp Ala Pro Ile 
                725                 730                 735     
Gly Val Glu Arg Gln 
            740     

