               SEQUENCE LISTING

<110> Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

<120> Method for the generation of a cDNA library from a pool of tRNAs

<130> AE2632 PCT

<160> 25

<170> BiSSAP 1.3.6

<210> 1
<211> 562
<212> PRT
<213> Thermosynechococcus elongatus


<400> 1
Met Glu Thr Arg Gln Met Thr Val Asp Gln Thr Thr Gly Ala Val Thr 
1               5                   10                  15      
Asn Gln Thr Glu Thr Ser Trp His Ser Ile Asn Trp Thr Lys Ala Asn 
            20                  25                  30          
Arg Glu Val Lys Arg Leu Gln Val Arg Ile Ala Lys Ala Val Lys Glu 
        35                  40                  45              
Gly Arg Trp Gly Lys Val Lys Ala Leu Gln Trp Leu Leu Thr His Ser 
    50                  55                  60                  
Phe Tyr Gly Lys Ala Leu Ala Val Lys Arg Val Thr Asp Asn Ser Gly 
65                  70                  75                  80  
Ser Arg Thr Pro Gly Val Asp Gly Ile Thr Trp Ser Thr Gln Glu Gln 
                85                  90                  95      
Lys Thr Gln Ala Ile Lys Ser Leu Arg Arg Arg Gly Tyr Lys Pro Gln 
            100                 105                 110         
Pro Leu Arg Arg Val Tyr Ile Pro Lys Ala Asn Gly Lys Gln Arg Pro 
        115                 120                 125             
Leu Gly Ile Pro Thr Met Lys Asp Arg Ala Met Gln Ala Leu Tyr Ala 
    130                 135                 140                 
Leu Ala Leu Glu Pro Val Ala Glu Thr Thr Ala Asp Arg Asn Ser Tyr 
145                 150                 155                 160 
Gly Phe Arg Arg Gly Arg Cys Thr Ala Asp Ala Ala Gly Gln Cys Phe 
                165                 170                 175     
Leu Ala Leu Ala Lys Ala Lys Ser Ala Glu His Val Leu Asp Ala Asp 
            180                 185                 190         
Ile Ser Gly Cys Phe Asp Asn Ile Ser His Glu Trp Leu Leu Ala Asn 
        195                 200                 205             
Thr Pro Leu Asp Lys Gly Ile Leu Arg Lys Trp Leu Lys Ser Gly Phe 
    210                 215                 220                 
Val Trp Lys Gln Gln Leu Phe Pro Thr His Ala Gly Thr Pro Gln Gly 
225                 230                 235                 240 
Gly Val Ile Ser Pro Val Leu Ala Asn Ile Thr Leu Asp Gly Met Glu 
                245                 250                 255     
Glu Leu Leu Ala Lys His Leu Arg Gly Gln Lys Val Asn Leu Ile Arg 
            260                 265                 270         
Tyr Ala Asp Asp Phe Val Val Thr Gly Lys Asp Glu Glu Thr Leu Glu 
        275                 280                 285             
Lys Ala Arg Asn Leu Ile Gln Glu Phe Leu Lys Glu Arg Gly Leu Thr 
    290                 295                 300                 
Leu Ser Pro Glu Lys Thr Lys Ile Val His Ile Glu Glu Gly Phe Asp 
305                 310                 315                 320 
Phe Leu Gly Trp Asn Ile Arg Lys Tyr Asn Gly Val Leu Leu Ile Lys 
                325                 330                 335     
Pro Ala Lys Lys Asn Val Lys Ala Phe Leu Lys Lys Ile Arg Asp Thr 
            340                 345                 350         
Leu Arg Glu Leu Arg Thr Ala Thr Gln Glu Ile Val Ile Asp Thr Leu 
        355                 360                 365             
Asn Pro Ile Ile Arg Gly Trp Ala Asn Tyr His Lys Gly Gln Val Ser 
    370                 375                 380                 
Lys Glu Thr Phe Asn Arg Val Asp Phe Ala Thr Trp His Lys Leu Trp 
385                 390                 395                 400 
Arg Trp Ala Arg Arg Arg His Pro Asn Lys Pro Ala Gln Trp Val Lys 
                405                 410                 415     
Asp Lys Tyr Phe Ile Lys Asn Gly Ser Arg Asp Trp Val Phe Gly Met 
            420                 425                 430         
Val Met Lys Asp Lys Asn Gly Glu Leu Arg Thr Lys Arg Leu Ile Lys 
        435                 440                 445             
Thr Ser Asp Thr Arg Ile Gln Arg His Val Lys Ile Lys Ala Asp Ala 
    450                 455                 460                 
Asn Pro Phe Leu Pro Glu Trp Ala Glu Tyr Phe Glu Lys Arg Lys Lys 
465                 470                 475                 480 
Leu Lys Lys Ala Pro Ala Gln Tyr Arg Arg Ile Arg Arg Glu Leu Trp 
                485                 490                 495     
Lys Lys Gln Gly Gly Ile Cys Pro Val Cys Gly Gly Glu Ile Glu Gln 
            500                 505                 510         
Asp Met Leu Thr Asp Ile His His Ile Leu Pro Lys His Lys Gly Gly 
        515                 520                 525             
Ser Asp Asp Leu Asp Asn Leu Val Leu Ile His Ala Asn Cys His Lys 
    530                 535                 540                 
Gln Val His Ser Arg Asp Gly Gln His Ser Arg Ser Leu Leu Lys Glu 
545                 550                 555                 560 
Gly Leu 
        

<210> 2
<211> 562
<212> PRT
<213> Thermosynechococcus elongatus


<400> 2
Met Glu Thr Arg Gln Met Ala Val Glu Gln Thr Thr Gly Ala Val Thr 
1               5                   10                  15      
Asn Gln Thr Glu Thr Ser Trp His Ser Ile Asp Trp Ala Lys Ala Asn 
            20                  25                  30          
Arg Glu Val Lys Arg Leu Gln Val Arg Ile Ala Lys Ala Val Lys Glu 
        35                  40                  45              
Gly Arg Trp Gly Lys Val Lys Ala Leu Gln Trp Leu Leu Thr His Ser 
    50                  55                  60                  
Phe Tyr Gly Lys Ala Leu Ala Val Lys Arg Val Thr Asp Asn Ser Gly 
65                  70                  75                  80  
Ser Lys Thr Pro Gly Val Asp Gly Ile Thr Trp Ser Thr Gln Glu Gln 
                85                  90                  95      
Lys Ala Gln Ala Ile Lys Ser Leu Arg Arg Arg Gly Tyr Lys Pro Gln 
            100                 105                 110         
Pro Leu Arg Arg Val Tyr Ile Pro Lys Ala Asn Gly Lys Gln Arg Pro 
        115                 120                 125             
Leu Gly Ile Pro Thr Met Lys Asp Arg Ala Met Gln Ala Leu Tyr Ala 
    130                 135                 140                 
Leu Ala Leu Glu Pro Val Ala Glu Thr Thr Ala Asp Arg Asn Ser Tyr 
145                 150                 155                 160 
Gly Phe Arg Arg Gly Arg Cys Ile Ala Asp Ala Ala Thr Gln Cys His 
                165                 170                 175     
Ile Thr Leu Ala Lys Thr Asp Arg Ala Gln Tyr Val Leu Asp Ala Asp 
            180                 185                 190         
Ile Ala Gly Cys Phe Asp Asn Ile Ser His Glu Trp Leu Leu Ala Asn 
        195                 200                 205             
Ile Pro Leu Asp Lys Arg Ile Leu Arg Lys Trp Leu Lys Ser Gly Phe 
    210                 215                 220                 
Val Trp Lys Gln Gln Leu Phe Pro Ile His Ala Gly Thr Pro Gln Gly 
225                 230                 235                 240 
Gly Val Ile Ser Pro Met Leu Ala Asn Met Thr Leu Asp Gly Met Glu 
                245                 250                 255     
Glu Leu Leu Asn Lys Phe Pro Arg Ala His Lys Val Lys Leu Ile Arg 
            260                 265                 270         
Tyr Ala Asp Asp Phe Val Val Thr Gly Glu Thr Lys Glu Val Leu Tyr 
        275                 280                 285             
Ile Ala Gly Ala Val Ile Gln Ala Phe Leu Lys Glu Arg Gly Leu Thr 
    290                 295                 300                 
Leu Ser Lys Glu Lys Thr Lys Ile Val His Ile Glu Glu Gly Phe Asp 
305                 310                 315                 320 
Phe Leu Gly Trp Asn Ile Arg Lys Tyr Asp Gly Lys Leu Leu Ile Lys 
                325                 330                 335     
Pro Ala Lys Lys Asn Val Lys Ala Phe Leu Lys Lys Ile Arg Asp Thr 
            340                 345                 350         
Leu Arg Glu Leu Arg Thr Ala Pro Gln Glu Ile Val Ile Asp Thr Leu 
        355                 360                 365             
Asn Pro Ile Ile Arg Gly Trp Thr Asn Tyr His Lys Asn Gln Ala Ser 
    370                 375                 380                 
Lys Glu Thr Phe Val Gly Val Asp His Leu Ile Trp Gln Lys Leu Trp 
385                 390                 395                 400 
Arg Trp Ala Arg Arg Arg His Pro Ser Lys Ser Val Arg Trp Val Lys 
                405                 410                 415     
Ser Lys Tyr Phe Ile Gln Ile Gly Asn Arg Lys Trp Met Phe Gly Ile 
            420                 425                 430         
Trp Thr Lys Asp Lys Asn Gly Asp Pro Trp Ala Lys His Leu Ile Lys 
        435                 440                 445             
Ala Ser Glu Ile Arg Ile Gln Arg Arg Gly Lys Ile Lys Ala Asp Ala 
    450                 455                 460                 
Asn Pro Phe Leu Pro Glu Trp Ala Glu Tyr Phe Glu Gln Arg Lys Lys 
465                 470                 475                 480 
Leu Lys Glu Ala Pro Ala Gln Tyr Arg Arg Thr Arg Arg Glu Leu Trp 
                485                 490                 495     
Lys Lys Gln Gly Gly Ile Cys Pro Val Cys Gly Gly Glu Ile Glu Gln 
            500                 505                 510         
Asp Met Leu Thr Glu Ile His His Ile Leu Pro Lys His Lys Gly Gly 
        515                 520                 525             
Thr Asp Asp Leu Asp Asn Leu Val Leu Ile His Thr Asn Cys His Lys 
    530                 535                 540                 
Gln Val His Asn Arg Asp Gly Gln His Ser Arg Phe Leu Leu Lys Glu 
545                 550                 555                 560 
Gly Leu 
        

<210> 3
<211> 562
<212> PRT
<213> Thermosynechococcus elongatus


<400> 3
Met Glu Thr Arg Gln Met Ala Val Glu Gln Thr Thr Gly Ala Val Thr 
1               5                   10                  15      
Asn Gln Thr Glu Thr Ser Trp His Ser Ile Asp Trp Ala Lys Ala Asn 
            20                  25                  30          
Arg Glu Val Lys Arg Leu Gln Val Arg Ile Ala Lys Ala Val Lys Glu 
        35                  40                  45              
Gly Arg Trp Gly Lys Val Lys Ala Leu Gln Trp Leu Leu Thr His Ser 
    50                  55                  60                  
Phe Tyr Gly Lys Ala Leu Ala Val Lys Arg Val Thr Asp Asn Ser Gly 
65                  70                  75                  80  
Ser Lys Thr Pro Gly Val Asp Gly Ile Thr Trp Ser Thr Gln Glu Gln 
                85                  90                  95      
Lys Ala Gln Ala Ile Lys Ser Leu Arg Arg Arg Gly Tyr Lys Pro Gln 
            100                 105                 110         
Pro Leu Arg Arg Val Tyr Ile Pro Lys Ala Ser Gly Lys Gln Arg Pro 
        115                 120                 125             
Leu Gly Ile Pro Thr Thr Lys Asp Arg Ala Met Gln Ala Leu Tyr Ala 
    130                 135                 140                 
Leu Ala Leu Glu Pro Val Ala Glu Thr Thr Ala Asp Arg Asn Ser Tyr 
145                 150                 155                 160 
Gly Phe Arg Gln Gly Arg Cys Thr Ala Asp Ala Ala Gly Gln Cys Phe 
                165                 170                 175     
Thr Val Leu Gly Arg Ser Asp Cys Ala Lys Tyr Ile Leu Asp Ala Asp 
            180                 185                 190         
Ile Thr Gly Cys Phe Asp Asn Ile Ser His Glu Trp Leu Leu Asp Asn 
        195                 200                 205             
Ile Pro Leu Asp Lys Glu Val Leu Arg Lys Trp Leu Lys Ser Gly Phe 
    210                 215                 220                 
Val Trp Lys Gln Gln Leu Phe Pro Thr His Ala Gly Thr Pro Gln Gly 
225                 230                 235                 240 
Gly Val Ile Ser Pro Met Leu Ala Asn Met Thr Leu Asp Gly Met Glu 
                245                 250                 255     
Glu Leu Leu Lys Lys His Leu Arg Lys Gln Lys Val Asn Leu Ile Arg 
            260                 265                 270         
Tyr Ala Asp Asp Phe Val Val Thr Gly Glu Ser Lys Glu Thr Leu Glu 
        275                 280                 285             
Lys Val Thr Thr Val Ile Gln Glu Phe Leu Lys Glu Arg Gly Leu Thr 
    290                 295                 300                 
Leu Ser Glu Glu Lys Thr Lys Val Val His Ile Glu Glu Gly Phe Asp 
305                 310                 315                 320 
Phe Leu Gly Trp Asn Ile Arg Lys Tyr Gly Glu Lys Leu Leu Ile Lys 
                325                 330                 335     
Pro Ala Lys Lys Asn Ile Lys Ala Phe His Lys Lys Ile Arg Asp Ala 
            340                 345                 350         
Leu Lys Glu Leu Arg Thr Ala Thr Gln Glu Ala Val Ile Asp Thr Leu 
        355                 360                 365             
Asn Pro Ile Ile Lys Gly Trp Ala Asn Tyr His Arg Asn Gln Val Ser 
    370                 375                 380                 
Lys Arg Ile Phe Asn Arg Ala Asp Asp Asn Ile Trp His Lys Leu Trp 
385                 390                 395                 400 
Arg Trp Ala Lys Arg Arg His Pro Asn Lys Pro Ala Arg Trp Thr Lys 
                405                 410                 415     
Asn Lys Tyr Phe Ile Lys Ile Gly Asn Arg His Trp Val Phe Gly Thr 
            420                 425                 430         
Trp Lys Lys Asp Lys Glu Gly Arg Leu Arg Ser Arg Tyr Leu Ile Lys 
        435                 440                 445             
Ala Gly Asp Thr Arg Ile Gln Arg His Val Lys Ile Lys Ala Asp Ala 
    450                 455                 460                 
Asn Pro Phe Leu Pro Glu Trp Ala Glu Tyr Phe Glu Glu Arg Lys Lys 
465                 470                 475                 480 
Leu Lys Glu Ala Pro Ala Gln Tyr Arg Arg Ile Arg Arg Glu Leu Trp 
                485                 490                 495     
Lys Lys Gln Gly Gly Ile Cys Pro Val Cys Gly Gly Glu Ile Glu Gln 
            500                 505                 510         
Asp Met Leu Thr Glu Ile His His Ile Leu Pro Lys His Lys Gly Gly 
        515                 520                 525             
Ser Asp Asp Leu Asp Asn Leu Val Leu Ile His Ala Asn Cys His Lys 
    530                 535                 540                 
Gln Val His Ser Arg Asp Gly Gln His Ser Arg Phe Leu Leu Lys Glu 
545                 550                 555                 560 
Gly Leu 
        

<210> 4
<211> 635
<212> PRT
<213> Geobacillus stearothermophilus


<400> 4
Met Lys Val Asn Lys Leu Val Val Lys Ser Glu Gln Asp Leu Arg Asn 
1               5                   10                  15      
Cys Leu Asp Leu Leu Tyr Gln Glu Ala Lys Lys Gly Lys His Phe Tyr 
            20                  25                  30          
Gly Met Leu Glu Leu Leu Gln Asn Asp Val Val Ile Leu Glu Ala Ile 
        35                  40                  45              
Arg Asn Ile Lys Ser Asn Lys Gly Ser Lys Thr Ala Gly Ile Asp Gln 
    50                  55                  60                  
Lys Ile Val Asp Asp Tyr Leu Leu Met Pro Thr Glu Lys Val Phe Gly 
65                  70                  75                  80  
Met Ile Lys Ala Lys Leu Asn Asp Tyr Lys Pro Ile Pro Val Arg Arg 
                85                  90                  95      
Cys Asn Lys Pro Lys Gly Asn Ala Lys Ser Ser Lys Arg Lys Gly Asn 
            100                 105                 110         
Ser Pro Asn Glu Glu Gly Glu Thr Arg Pro Leu Gly Ile Ser Ala Val 
        115                 120                 125             
Thr Asp Arg Ile Ile Gln Glu Met Leu Arg Ile Val Leu Glu Pro Ile 
    130                 135                 140                 
Phe Glu Ala Gln Phe Tyr Pro His Ser Tyr Gly Phe Arg Pro Tyr Arg 
145                 150                 155                 160 
Ser Thr Glu His Ala Leu Ala Trp Met Leu Lys Ile Ile Asn Gly Ser 
                165                 170                 175     
Lys Leu Tyr Trp Val Val Lys Gly Asp Ile Glu Ser Tyr Phe Asp His 
            180                 185                 190         
Ile Asn His Lys Lys Leu Leu Asn Ile Met Trp Asn Met Gly Val Arg 
        195                 200                 205             
Asp Lys Arg Val Leu Cys Ile Val Lys Lys Met Leu Lys Ala Gly Gln 
    210                 215                 220                 
Val Ile Gln Gly Lys Phe Tyr Pro Thr Ala Lys Gly Ile Pro Gln Gly 
225                 230                 235                 240 
Gly Ile Ile Ser Pro Leu Leu Ala Asn Val Tyr Leu Asn Ser Phe Asp 
                245                 250                 255     
Trp Met Val Gly Gln Glu Tyr Glu Tyr His Pro Asn Asn Ala Asn Tyr 
            260                 265                 270         
Arg Glu Lys Lys Asn Ala Leu Ala Ala Leu Arg Asn Lys Gly His His 
        275                 280                 285             
Pro Val Phe Tyr Ile Arg Tyr Ala Asp Asp Trp Val Ile Leu Thr Asp 
    290                 295                 300                 
Thr Lys Glu Tyr Ala Glu Lys Ile Arg Glu Gln Cys Lys Gln Tyr Leu 
305                 310                 315                 320 
Ala Cys Glu Leu His Leu Thr Leu Ser Asp Glu Lys Thr Phe Ile Ala 
                325                 330                 335     
Asp Ile Arg Glu Gln Arg Val Lys Phe Leu Gly Phe Cys Ile Glu Ala 
            340                 345                 350         
Gly Lys Arg Arg Phe His Lys Lys Gly Phe Ala Ala Arg Met Ile Pro 
        355                 360                 365             
Asp Met Glu Lys Val Asn Ala Lys Val Lys Glu Ile Lys Arg Asp Ile 
    370                 375                 380                 
Arg Leu Leu Arg Thr Arg Lys Ser Glu Leu Glu Lys Ala Leu Asp Ile 
385                 390                 395                 400 
Glu Asn Ile Asn Thr Lys Ile Ile Gly Leu Ala Asn His Leu Lys Ile 
                405                 410                 415     
Gly Ile Ser Lys Tyr Ile Met Gly Lys Val Asp Arg Val Ile Glu Glu 
            420                 425                 430         
Thr Ala Tyr Arg Thr Trp Val Lys Met Tyr Gly Lys Glu Lys Ala Ala 
        435                 440                 445             
Gln Tyr Lys Arg Pro Val Ser Glu Phe His Asn Arg Ile Asp Arg His 
    450                 455                 460                 
Lys Gly Tyr Gln Met Lys His Phe Ser Val Val Thr Glu Asp Gly Ile 
465                 470                 475                 480 
Arg Val Gly Ile Thr His Ala Lys Ile Thr Pro Ile Gln Tyr Ala Thr 
                485                 490                 495     
Val Phe Lys Gln Glu Met Thr Pro Tyr Thr Ala Asp Gly Arg Lys Met 
            500                 505                 510         
Tyr Glu Glu Lys His Arg Lys Ile Arg Leu Pro Asp Lys Met Ser Leu 
        515                 520                 525             
Phe Asp His Asp Ser Ile Phe Ile Tyr Ile Leu Ser Glu His Asn Asp 
    530                 535                 540                 
Gly Lys Tyr Asn Leu Glu Tyr Phe Leu Asn Arg Val Asn Val Phe His 
545                 550                 555                 560 
Arg Asp Lys Gly Lys Cys Lys Ile Cys Ala Val Tyr Leu Ser Pro Gly 
                565                 570                 575     
Asn Phe His Cys His His Ile Asp Pro Ser Lys Pro Leu Ser Glu Ile 
            580                 585                 590         
Asn Lys Thr Val Asn Leu Ile Ser Leu Cys Asn Gln Cys His Arg Leu 
        595                 600                 605             
Val His Ser Asn Gln Glu Pro Pro Phe Thr Glu Arg Lys Met Phe Asp 
    610                 615                 620                 
Lys Leu Thr Lys Tyr Arg Asn Lys Leu Lys Ile 
625                 630                 635 

<210> 5
<211> 420
<212> PRT
<213> Geobacillus stearothermophilus


<400> 5
Met Ala Leu Leu Glu Arg Ile Leu Ala Arg Asp Asn Leu Ile Thr Ala 
1               5                   10                  15      
Leu Lys Arg Val Glu Ala Asn Gln Gly Ala Pro Gly Ile Asp Gly Val 
            20                  25                  30          
Ser Thr Asp Gln Leu Arg Asp Tyr Ile Arg Ala His Trp Ser Thr Ile 
        35                  40                  45              
His Ala Gln Leu Leu Ala Gly Thr Tyr Arg Pro Ala Pro Val Arg Arg 
    50                  55                  60                  
Val Glu Ile Pro Lys Pro Gly Gly Gly Thr Arg Gln Leu Gly Ile Pro 
65                  70                  75                  80  
Thr Val Val Asp Arg Leu Ile Gln Gln Ala Ile Leu Gln Glu Leu Thr 
                85                  90                  95      
Pro Ile Phe Asp Pro Asp Phe Ser Ser Ser Ser Phe Gly Phe Arg Pro 
            100                 105                 110         
Gly Arg Asn Ala His Asp Ala Val Arg Gln Ala Gln Gly Tyr Ile Gln 
        115                 120                 125             
Glu Gly Tyr Arg Tyr Val Val Asp Met Asp Leu Glu Lys Phe Phe Asp 
    130                 135                 140                 
Arg Val Asn His Asp Ile Leu Met Ser Arg Val Ala Arg Lys Val Lys 
145                 150                 155                 160 
Asp Lys Arg Val Leu Lys Leu Ile Arg Ala Tyr Leu Gln Ala Gly Val 
                165                 170                 175     
Met Ile Glu Gly Val Lys Val Gln Thr Glu Glu Gly Thr Pro Gln Gly 
            180                 185                 190         
Gly Pro Leu Ser Pro Leu Leu Ala Asn Ile Leu Leu Asp Asp Leu Asp 
        195                 200                 205             
Lys Glu Leu Glu Lys Arg Gly Leu Lys Phe Cys Arg Tyr Ala Asp Asp 
    210                 215                 220                 
Cys Asn Ile Tyr Val Lys Ser Leu Arg Ala Gly Gln Arg Val Lys Gln 
225                 230                 235                 240 
Ser Ile Gln Arg Phe Leu Glu Lys Thr Leu Lys Leu Lys Val Asn Glu 
                245                 250                 255     
Glu Lys Ser Ala Val Asp Arg Pro Trp Lys Arg Ala Phe Leu Gly Phe 
            260                 265                 270         
Ser Phe Thr Pro Glu Arg Lys Ala Arg Ile Arg Leu Ala Pro Arg Ser 
        275                 280                 285             
Ile Gln Arg Leu Lys Gln Arg Ile Arg Gln Leu Thr Asn Pro Asn Trp 
    290                 295                 300                 
Ser Ile Ser Met Pro Glu Arg Ile His Arg Val Asn Gln Tyr Val Met 
305                 310                 315                 320 
Gly Trp Ile Gly Tyr Phe Arg Leu Val Glu Thr Pro Ser Val Leu Gln 
                325                 330                 335     
Thr Ile Glu Gly Trp Ile Arg Arg Arg Leu Arg Leu Cys Gln Trp Leu 
            340                 345                 350         
Gln Trp Lys Arg Val Arg Thr Arg Ile Arg Glu Leu Arg Ala Leu Gly 
        355                 360                 365             
Leu Lys Glu Thr Ala Val Met Glu Ile Ala Asn Thr Arg Lys Gly Ala 
    370                 375                 380                 
Trp Arg Thr Thr Lys Thr Pro Gln Leu His Gln Ala Leu Gly Lys Thr 
385                 390                 395                 400 
Tyr Trp Thr Ala Gln Gly Leu Lys Ser Leu Thr Gln Arg Tyr Phe Glu 
                405                 410                 415     
Leu Arg Gln Gly 
            420 

<210> 6
<211> 427
<212> PRT
<213> Eubacterium rectale


<400> 6
Met Asp Thr Ser Asn Leu Met Glu Gln Ile Leu Ser Ser Asp Asn Leu 
1               5                   10                  15      
Asn Arg Ala Tyr Leu Gln Val Val Arg Asn Lys Gly Ala Glu Gly Val 
            20                  25                  30          
Asp Gly Met Lys Tyr Thr Glu Leu Lys Glu His Leu Ala Lys Asn Gly 
        35                  40                  45              
Glu Thr Ile Lys Gly Gln Leu Arg Thr Arg Lys Tyr Lys Pro Gln Pro 
    50                  55                  60                  
Ala Arg Arg Val Glu Ile Pro Lys Pro Asp Gly Gly Val Arg Asn Leu 
65                  70                  75                  80  
Gly Val Pro Thr Val Thr Asp Arg Phe Ile Gln Gln Ala Ile Ala Gln 
                85                  90                  95      
Val Leu Thr Pro Ile Tyr Glu Glu Gln Phe His Asp His Ser Tyr Gly 
            100                 105                 110         
Phe Arg Pro Asn Arg Cys Ala Gln Gln Ala Ile Leu Thr Ala Leu Asn 
        115                 120                 125             
Ile Met Asn Asp Gly Asn Asp Trp Ile Val Asp Ile Asp Leu Glu Lys 
    130                 135                 140                 
Phe Phe Asp Thr Val Asn His Asp Lys Leu Met Thr Leu Ile Gly Arg 
145                 150                 155                 160 
Thr Ile Lys Asp Gly Asp Val Ile Ser Ile Val Arg Lys Tyr Leu Val 
                165                 170                 175     
Ser Gly Ile Met Ile Asp Asp Glu Tyr Glu Asp Ser Ile Val Gly Thr 
            180                 185                 190         
Pro Gln Gly Gly Asn Leu Ser Pro Leu Leu Ala Asn Ile Met Leu Asn 
        195                 200                 205             
Glu Leu Asp Lys Glu Met Glu Lys Arg Gly Leu Asn Phe Val Arg Tyr 
    210                 215                 220                 
Ala Asp Asp Cys Ile Ile Met Val Gly Ser Glu Met Ser Ala Asn Arg 
225                 230                 235                 240 
Val Met Arg Asn Ile Ser Arg Phe Ile Glu Glu Lys Leu Gly Leu Lys 
                245                 250                 255     
Val Asn Met Thr Lys Ser Lys Val Asp Arg Pro Ser Gly Leu Lys Tyr 
            260                 265                 270         
Leu Gly Phe Gly Phe Tyr Phe Asp Pro Arg Ala His Gln Phe Lys Ala 
        275                 280                 285             
Lys Pro His Ala Lys Ser Val Ala Lys Phe Lys Lys Arg Met Lys Glu 
    290                 295                 300                 
Leu Thr Cys Arg Ser Trp Gly Val Ser Asn Ser Tyr Lys Val Glu Lys 
305                 310                 315                 320 
Leu Asn Gln Leu Ile Arg Gly Trp Ile Asn Tyr Phe Lys Ile Gly Ser 
                325                 330                 335     
Met Lys Thr Leu Cys Lys Glu Leu Asp Ser Arg Ile Arg Tyr Arg Leu 
            340                 345                 350         
Arg Met Cys Ile Trp Lys Gln Trp Lys Thr Pro Gln Asn Gln Glu Lys 
        355                 360                 365             
Asn Leu Val Lys Leu Gly Ile Asp Arg Asn Thr Ala Arg Arg Val Ala 
    370                 375                 380                 
Tyr Thr Gly Lys Arg Ile Ala Tyr Val Cys Asn Lys Gly Ala Val Asn 
385                 390                 395                 400 
Val Ala Ile Ser Asn Lys Arg Leu Ala Ser Phe Gly Leu Ile Ser Met 
                405                 410                 415     
Leu Asp Tyr Tyr Ile Glu Lys Cys Val Thr Cys 
            420                 425         

<210> 7
<211> 76
<212> DNA
<213> Artificial Sequence


<220> 
<223> /note="combined DNA/RNA molecule"
      /note="E.coli tRNA-Lys-UUU with intact 3′-CCA"

<400> 7
gggucguuag cucaguuggu agagcaguug acuuuuaauc aauuggucgc agguucgaau     60

ccugcacgac ccacca                                                     76


<210> 8
<211> 75
<212> DNA
<213> Artificial Sequence


<220> 
<223> /note="combined DNA/RNA molecule"
      /note="E.coli tRNA-Lys-UUU with intact 3′-CC"

<400> 8
gggucguuag cucaguuggu agagcaguug acuuuuaauc aauuggucgc agguucgaau     60

ccugcacgac ccacc                                                      75


<210> 9
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> barcode l1

<400> 9
gatatcgtca agatcggaag agcacacgtc tgaa                                 34


<210> 10
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> barcode l2

<400> 10
gatagctaca agatcggaag agcacacgtc tgaa                                 34


<210> 11
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> barcode l3

<400> 11
gatgcataca agatcggaag agcacacgtc tgaa                                 34


<210> 12
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> barcode l4

<400> 12
gattctagca agatcggaag agcacacgtc tgaa                                 34


<210> 13
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> /note="combined DNA/RNA molecule"
      /note="RNA oligonucleotide "

<400> 13
gagcacacgu cugaacucca cucuuucccu acacgacgcu cuuccgaucu                50


<210> 14
<211> 52
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA oligonucleotide

<220> 
<221> variation
<222> 52
<223> /replace="a,c,g,t"

<400> 14
ragatcggaa gagcgtcgtg tagggaaaga gtggagttca gacgtgtgct cn             52


<210> 15
<211> 57
<212> DNA
<213> Artificial Sequence


<220> 
<223> RT primer

<220> 
<221> variation
<222> 2
<223> /replace="a,c,g,t"

<400> 15
rnagatcgga agagcgtcgt gtagggaaag aggtgactgg agttcagacg tgtgctc        57


<210> 16
<211> 49
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer

<400> 16
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctc                 49


<210> 17
<211> 52
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer

<220> 
<221> variation
<222> 25,26,27,28,29,30
<223> /replace="a,c,g,t"

<400> 17
caagcagaag acggcatacg agatnnnnnn gtgactggag ttcagacgtg tg             52


<210> 18
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5′-end 32P-labeled probe

<400> 18
cggaacctct ggattagaag tccagcgcgc tcgtcc                               36


<210> 19
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Gly-CCC-2

<400> 19
cgggtcgcaa gaatgggaat cttgcatgat ac                                   32


<210> 20
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> tRNA-Leu-UAA

<400> 20
cgcggacaac cgtccaac                                                  18


<210> 21
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> tRNA-Pro-UGG

<400> 21
tgaacccagg gcctct                                                    16


<210> 22
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> tRNA isotype

<400> 22
Phe Lys Tyr Ile Leu Met Arg Asp Ala Ser Thr Glu Met Asn Gly Val 
1               5                   10                  15      
His Pro Trp Gln Cys 
            20      

<210> 23
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> tRNA isotype

<400> 23
Ile Cys Phe Ala Tyr Arg Asp Glu Leu Lys Trp His Ser Pro Thr Met 
1               5                   10                  15      
Asn Val Gly Gln 
            20  

<210> 24
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> tRNA isotype

<400> 24
Val Leu Tyr Met Phe Lys Arg Cys Ala Ser Trp Thr Asn Ile Glu Pro 
1               5                   10                  15      
Gln Asp Gly His Met 
            20      

<210> 25
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> tRNA isotype

<400> 25
Tyr Lys Phe Thr Asn Trp Asp Ile Cys Met Arg Pro Ala Val Gly Gln 
1               5                   10                  15      
Leu Ser Met Glu 
            20  

