﻿                         序列表

<110>  上海鑫湾生物科技有限公司

<120>  缺氧触发的人工转录因子、转录控制系统及其应用

<130>  EIC21310017P

<150>  2021109302685
<151>  2021-08-13

<160>  9

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  661
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  1
Met His His His His His His Pro Lys Lys Lys Arg Lys Val Ser Asn 
1               5                   10                  15      
Leu Leu Thr Val His Gln Asn Leu Pro Ala Leu Pro Val Asp Ala Thr 
            20                  25                  30          
Ser Asp Glu Val Arg Lys Asn Leu Met Asp Met Phe Arg Asp Arg Gln 
        35                  40                  45              
Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val Cys Arg Ser 
    50                  55                  60                  
Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe Pro Ala Glu 
65                  70                  75                  80  
Pro Glu Asp Val Arg Asp Tyr Leu Leu Tyr Leu Gln Ala Arg Gly Leu 
                85                  90                  95      
Ala Val Lys Thr Ile Gln Gln His Leu Gly Gln Leu Asn Met Leu His 
            100                 105                 110         
Arg Arg Ser Gly Leu Pro Arg Pro Ser Asp Ser Asn Ala Val Ser Leu 
        115                 120                 125             
Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly Glu Arg Ala 
    130                 135                 140                 
Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln Val Arg Ser 
145                 150                 155                 160 
Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn Leu Ala Phe 
                165                 170                 175     
Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ala Glu Ile Ala Arg 
            180                 185                 190         
Ile Arg Val Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg Met Leu Ile 
        195                 200                 205             
His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly Val Glu Lys 
    210                 215                 220                 
Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp Ile Ser Val 
225                 230                 235                 240 
Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys Arg Val Arg 
                245                 250                 255     
Lys Asn Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu Ser Thr Arg 
            260                 265                 270         
Ala Leu Glu Gly Ile Phe Glu Ala Thr His Arg Leu Ile Tyr Gly Ala 
        275                 280                 285             
Lys Asp Asp Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly His Ser Ala 
    290                 295                 300                 
Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val Ser Ile Pro 
305                 310                 315                 320 
Glu Ile Met Gln Ala Gly Gly Trp Thr Asn Val Asn Ile Val Met Asn 
                325                 330                 335     
Phe Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val Arg Leu Leu 
            340                 345                 350         
Glu Asp Gly Asp Ser Arg Ala Ala Ala Gly Gly Ser Gly Gly Ser Gly 
        355                 360                 365             
Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp 
    370                 375                 380                 
Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp 
385                 390                 395                 400 
Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp 
                405                 410                 415     
Leu Asp Met Leu Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            420                 425                 430         
Gly Gly Gly Gly Ser Ser Glu Asp Thr Ser Ser Leu Phe Asp Lys Leu 
        435                 440                 445             
Lys Lys Glu Pro Asp Ala Leu Thr Leu Leu Ala Pro Ala Ala Gly Asp 
    450                 455                 460                 
Thr Ile Ile Ser Leu Asp Phe Gly Ser Asn Asp Thr Glu Thr Asp Asp 
465                 470                 475                 480 
Gln Gln Leu Glu Glu Val Pro Leu Tyr Asn Asp Val Met Leu Pro Ser 
                485                 490                 495     
Pro Asn Glu Lys Leu Gln Asn Ile Asn Leu Ala Met Ser Pro Leu Pro 
            500                 505                 510         
Thr Ala Glu Thr Pro Lys Pro Leu Arg Ser Ser Ala Asp Pro Ala Leu 
        515                 520                 525             
Asn Gln Glu Val Ala Leu Lys Leu Glu Pro Asn Pro Glu Ser Leu Glu 
    530                 535                 540                 
Leu Ser Phe Thr Met Pro Gln Ile Gln Asp Gln Thr Pro Ser Pro Ser 
545                 550                 555                 560 
Asp Gly Ser Thr Arg Gln Ser Ser Pro Glu Pro Asn Ser Pro Ser Glu 
                565                 570                 575     
Tyr Cys Phe Tyr Val Asp Ser Asp Met Val Asn Glu Phe Lys Leu Glu 
            580                 585                 590         
Leu Val Glu Lys Leu Phe Ala Glu Asp Thr Glu Ala Lys Asn Pro Phe 
        595                 600                 605             
Ser Thr Gln Asp Thr Asp Leu Asp Leu Glu Met Leu Ala Pro Tyr Ile 
    610                 615                 620                 
Pro Met Asp Asp Asp Phe Gln Leu Arg Ser Phe Asp Gln Leu Ser Pro 
625                 630                 635                 640 
Leu Glu Ser Ser Ser Ala Ser Pro Glu Ser Ala Ser Pro Gln Ser Thr 
                645                 650                 655     
Val Thr Val Phe Gln 
            660     

<210>  2
<211>  34
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  2
ataacttcgt ataatgtatg ctatacgaag ttat                                    34

<210>  3
<211>  269
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  3
taacttcgta taatgtatgc tatacgaagt tatataactt cgtataatgt atgctatacg        60
aagttatata acttcgtata atgtatgcta tacgaagtta taaagtgaaa gtcgagctcg       120
gtacccgggt cgactgcata taagcagctg ctctgcatat aagcagctgc tttttgcctg       180
tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa       240
cccactgctt aagcctcaat aaagcttgc                                         269

<210>  4
<211>  35
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  4
ccacagtgca tacgtgggct ccaacaggtc ctctt                                   35

<210>  5
<211>  175
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  5
ccacagtgca tacgtgggct ccaacaggtc ctcttccaca gtgcatacgt gggctccaac        60
aggtcctctt ccacagtgca tacgtgggct ccaacaggtc ctcttccaca gtgcatacgt       120
gggctccaac aggtcctctt ccacagtgca tacgtgggct ccaacaggtc ctctt            175

<210>  6
<211>  236
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  6
Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      
Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          
Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              
Thr Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  
Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  
Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      
Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         
Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             
Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 
Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 
Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     
His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         
Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             
His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 
Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235     

<210>  7
<211>  498
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  7
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      
His Ala Ala Arg Pro Asp Tyr Lys Asp Asp Asp Asp Lys Asp Ile Gln 
            20                  25                  30          
Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val 
        35                  40                  45              
Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala Val Ala Trp 
    50                  55                  60                  
Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala 
65                  70                  75                  80  
Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Arg Ser 
                85                  90                  95      
Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe 
            100                 105                 110         
Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro Thr Phe Gly 
        115                 120                 125             
Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Gly Ser Thr Ser Gly Ser 
    130                 135                 140                 
Gly Lys Pro Gly Ser Gly Glu Gly Ser Glu Val Gln Leu Val Glu Ser 
145                 150                 155                 160 
Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala 
                165                 170                 175     
Ala Ser Gly Phe Asn Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln 
            180                 185                 190         
Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn 
        195                 200                 205             
Gly Tyr Thr Arg Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser 
    210                 215                 220                 
Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg 
225                 230                 235                 240 
Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly 
                245                 250                 255     
Phe Tyr Ala Met Asp Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
            260                 265                 270         
Ser Thr Arg Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
        275                 280                 285             
Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
    290                 295                 300                 
Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
305                 310                 315                 320 
Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
                325                 330                 335     
Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu 
            340                 345                 350         
Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
        355                 360                 365             
Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
    370                 375                 380                 
Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys 
385                 390                 395                 400 
Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
                405                 410                 415     
Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
            420                 425                 430         
Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
        435                 440                 445             
Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
    450                 455                 460                 
Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
465                 470                 475                 480 
Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
                485                 490                 495     
Pro Arg 
        

<210>  8
<211>  520
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  8
Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      
Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
            20                  25                  30          
Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Thr Ile Ser Asp Tyr 
        35                  40                  45              
Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
    50                  55                  60                  
Lys Phe Ala Ser Gln Ser Ile Ser Gly Ile Pro Ala Arg Phe Ser Gly 
65                  70                  75                  80  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro 
                85                  90                  95      
Glu Asp Phe Ala Val Tyr Tyr Cys Gln Asn Gly His Gly Phe Pro Arg 
            100                 105                 110         
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly Ser 
        115                 120                 125             
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln 
    130                 135                 140                 
Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys 
145                 150                 155                 160 
Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Arg 
                165                 170                 175     
Gln Ala Pro Gly Gln Gly Leu Glu Trp Met Gly Tyr Ile Asn Pro Ser 
            180                 185                 190         
Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe Lys Asp Arg Val Thr Ile 
        195                 200                 205             
Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu 
    210                 215                 220                 
Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp 
225                 230                 235                 240 
His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser 
                245                 250                 255     
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            260                 265                 270         
Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser 
        275                 280                 285             
Pro Gly Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser Val Ser 
    290                 295                 300                 
Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Leu 
305                 310                 315                 320 
Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser 
                325                 330                 335     
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu 
            340                 345                 350         
Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro 
        355                 360                 365             
Phe Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Gly Gly Gly 
    370                 375                 380                 
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu 
385                 390                 395                 400 
Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu 
                405                 410                 415     
Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Gly Tyr Gly Met Ser Trp 
            420                 425                 430         
Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Thr Ile Thr 
        435                 440                 445             
Ser Gly Gly Thr Tyr Thr Tyr Tyr Pro Asp Ser Val Lys Gly Arg Phe 
    450                 455                 460                 
Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn 
465                 470                 475                 480 
Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ser Leu 
                485                 490                 495     
Ala Gly Asn Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
            500                 505                 510         
Ser Ser His His His His His His 
        515                 520 

<210>  9
<211>  2539
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  9
tcactggaac acggtcacgg ttgactgtgg tgaagcgctc tcaggacttg cgctagaaga        60
ttcgagaggg gacagctgat caaatgaccg cagctgaaag tcatcgtcca taggaatgta       120
aggtgcgagc atttctaagt ccaggtcggt gtcctgtgtt gaaaatgggt tctttgcctc       180
agtatcctct gcgaacagct tctcgactaa ctccagctta aactcattca ccatgtcgct       240
atcgacgtag aagcagtact cggatgggga attgggctca ggggaagact gcctagttga       300
tccgtcggat ggagatggag tctgatcctg aatctgtggc atagtaaatg aaagctccag       360
agactctggg tttggctcta gcttcagggc cacctcctgg ttcagagcgg gatcggcgct       420
agacctgagt ggcttaggtg tctcggcggt aggcagtggt gacatagcca gattaatatt       480
ttgcagcttc tcattaggag aaggcagcat gacatcattg tacagaggca cctcctccag       540
ctgctggtcg tcggtctctg tatcgttaga cccaaagtcc aggctaataa ttgtatcgcc       600
ggcggcgggt gcgaggagtg tgagtgcgtc gggctccttc ttgagcttgt caaacagtga       660
tgaggtatcc tcgctggacc ctccgccgcc ggagcccccg cccccggacc cgcctccgcc       720
tgatccgagc atgtccaggt caaagtcatc cagtgcatct gatcccaaca tgtcgagatc       780
gaaatcgtcc agagcgtcag atcccagcat gtccagatca aaatcatcca gggcgtcgct       840
gcccagcata tccaggtcga agtcgtccag ggcatcagat ccgccagagc ctccgcttcc       900
gcctgcggca gctctagaat cgccgtcctc gagcaggcgc accatagccc cggtctcaga       960
atcgaggttt ctgatgaagt tcatcacaat attcacattg gtccatcccc ctgcctgcat      1020
aatctctggg atagagaccc cggctctagc catatcgcgg gcggctccca ctctagctga      1080
gtggccggac caagccaggt accgctgccc gctatcgtcc ttggccccgt aaatgagtct      1140
gtgggttgcc tcgaaaattc cctccagtgc cctagtagac agctgtgaag ttgcagaagg      1200
ggcagcgacg ccgttcttcc gcactctaca aaacaggtaa ttattagggt cgtcggcgac      1260
gccggacacg ctaatccacc gctcgaccag ctttgtcact cccagtgaca gagccttctc      1320
gacgccagca gtagacacga gtgtctttgt gcgtccgatg tggatgagca tgcgccctcc      1380
gtcagtgcgt gaaatatcct tgactctgat cctagcgatc tctgcaatgc gcaggagggt      1440
gttgtatgca attcccagaa aggcgagatt ccgaatgtcc tggcaccgat cagagttctc      1500
catgagggat ctcacctgat caaagtcggt ccgctcaaag gcgagggcct gcttagcgcg      1560
ctcgccagcg tcgacgttct cctttctaat gcgtctcatg acgagtgaca cggcattgga      1620
atcagatggt ctagggaggc cggaccggcg gtggagcatg ttcagctggc cgaggtgctg      1680
ctgaatagtc ttgactgcca gtcccctagc ctggaggtac agcaggtagt cgcgcacatc      1740
ctcgggctcg gctgggaacc acttgcggtt attgagctta caccaagcgg cccaagacct      1800
acacacggac aggagcatct tccaagtgtg ctcgctaaaa gcctgtctat cgcgaaacat      1860
atccatgaga ttcttccgca cctcatcgga agttgcatcc acggggagag cgggcagatt      1920
ctggtggact gtcaggagat tagagacctt gcgcttcttc ttagggtggt ggtggtggtg      1980
gtgcatctgc agaccggtgg atcccagcac agtctccagg cgatctgacg gttcactaaa      2040
cgagctctgc ttatataggc ctcccaccgt acacgccacc tcgacatacc acagtgcata      2100
cgtgggctcc aacaggtcct cttccacagt gcatacgtgg gctccaacag gtcctcttcc      2160
acagtgcata cgtgggctcc aacaggtcct cttccacagt gcatacgtgg gctccaacag      2220
gtcctcttcc acagtgcata cgtgggctcc aacaggtcct cttatgcata taacttcgta      2280
taatgtatgc tatacgaagt tatataactt cgtataatgt atgctatacg aagttatata      2340
acttcgtata atgtatgcta tacgaagtta taaagtgaaa gtcgagctcg gtacccgggt      2400
cgactgcata taagcagctg ctctgcatat aagcagctgc tttttgcctg tactgggtct      2460
ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt      2520
aagcctcaat aaagcttgc                                                   2539

