﻿                         序列表

<110>  国家卫生健康委科学技术研究所

<120>  一种无PAM限制的腺嘌呤碱基编辑器融合蛋白及应用

<130>  PT20211896

<150>   CN 202110912085.0
<151>  2021-08-10

<160>  178

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  1046
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  SpRY(D10A)蛋白N端片段的氨基酸序列


<400>  1
Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val Gly 
1               5                   10                  15      
Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys 
            20                  25                  30          
Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly 
        35                  40                  45              
Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Arg Thr Arg Leu Lys 
    50                  55                  60                  
Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr 
65                  70                  75                  80  
Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe 
                85                  90                  95      
Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His 
            100                 105                 110         
Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His 
        115                 120                 125             
Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser 
    130                 135                 140                 
Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met 
145                 150                 155                 160 
Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp 
                165                 170                 175     
Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn 
            180                 185                 190         
Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys 
        195                 200                 205             
Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu 
    210                 215                 220                 
Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu 
225                 230                 235                 240 
Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp 
                245                 250                 255     
Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp 
            260                 265                 270         
Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu 
        275                 280                 285             
Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile 
    290                 295                 300                 
Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met 
305                 310                 315                 320 
Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala 
                325                 330                 335     
Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp 
            340                 345                 350         
Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln 
        355                 360                 365             
Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly 
    370                 375                 380                 
Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys 
385                 390                 395                 400 
Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly 
                405                 410                 415     
Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu 
            420                 425                 430         
Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro 
        435                 440                 445             
Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met 
    450                 455                 460                 
Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val 
465                 470                 475                 480 
Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn 
                485                 490                 495     
Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu 
            500                 505                 510         
Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr 
        515                 520                 525             
Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys 
    530                 535                 540                 
Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     
Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala His 
625                 630                 635                 640 
Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe Lys 
    690                 695                 700                 
Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu His 
705                 710                 715                 720 
Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly Arg 
            740                 745                 750         
His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             
Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu 
    770                 775                 780                 
Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 
Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     
Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu 
            820                 825                 830         
Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys Asp 
        835                 840                 845             
Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 
Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn 
865                 870                 875                 880 
Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     
Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys 
            900                 905                 910         
Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             
His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu 
    930                 935                 940                 
Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 
Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu 
                965                 970                 975     
Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         
Gly Thr Ala Leu Ile Lys Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val 
        995                 1000                 1005             
Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys Ser 
    1010                 1015                 1020                 
Glu  Gln Glu Ile Gly Lys  Ala Thr Ala Lys Tyr  Phe Phe Tyr Ser Asn  
1025                 1030                 1035                 1040 
Ile Met Asn Phe Phe  Lys 
                1045     

<210>  2
<211>  3138
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  编码所述SpRY(D10A)蛋白N端片段的核苷酸序列


<400>  2
gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc        60
accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac       120
agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgagaga       180
acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat       240
ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg       300
gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac       360
atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa       420
ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg       480
atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg       540
gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc       600
aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg       660
ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg       720
attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat       780
gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag       840
atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg       900
ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg       960
atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag      1020
cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc      1080
tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa      1140
aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag      1200
cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc      1260
attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag      1320
aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga      1380
ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg      1440
gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac      1500
ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat      1560
aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc      1620
ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg      1680
aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc      1740
ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc      1800
aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg      1860
accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac      1920
ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg      1980
ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat      2040
ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc      2100
ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac      2160
gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg      2220
aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc      2280
gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg      2340
aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg      2400
gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat      2460
atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc      2520
gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac      2580
aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac      2640
tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc      2700
aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg      2760
gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact      2820
aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag      2880
ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac      2940
caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac      3000
cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg      3060
atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac      3120
atcatgaact ttttcaag                                                    3138

<210>  3
<211>  166
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  TadA8e片段的氨基酸序列


<400>  3
Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu Thr 
1               5                   10                  15      
Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala Val 
            20                  25                  30          
Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala Ile 
        35                  40                  45              
Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg Gln 
    50                  55                  60                  
Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr 
65                  70                  75                  80  
Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His Ser 
                85                  90                  95      
Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ser Lys Arg Gly Ala 
            100                 105                 110         
Ala Gly Ser Leu Met Asn Val Leu Asn Tyr Pro Gly Met Asn His Arg 
        115                 120                 125             
Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu 
    130                 135                 140                 
Cys Asp Phe Tyr Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys Lys 
145                 150                 155                 160 
Ala Gln Ser Ser Ile Asn 
                165     

<210>  4
<211>  498
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  编码所述TadA8e片段的核苷酸序列


<400>  4
tctgaggtgg agttttccca cgagtactgg atgagacatg ccctgaccct ggccaagagg        60
gcacgggatg agagggaggt gcctgtggga gccgtgctgg tgctgaacaa tagagtgatc       120
ggcgagggct ggaacagagc catcggcctg cacgacccaa cagcccatgc cgaaattatg       180
gccctgagac agggcggcct ggtcatgcag aactacagac tgattgacgc caccctgtac       240
gtgacattcg agccttgcgt gatgtgcgcc ggcgccatga tccactctag gatcggccgc       300
gtggtgtttg gcgtgaggaa ctcaaaaaga ggcgccgcag gctccctgat gaacgtgctg       360
aactaccccg gcatgaatca ccgcgtcgaa attaccgagg gaatcctggc agatgaatgt       420
gccgccctgc tgtgcgattt ctatcggatg cctagacagg tgttcaatgc tcagaagaag       480
gcccagagct ccatcaac                                                     498

<210>  5
<211>  305
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  SpRY(D10A)蛋白C端片段的氨基酸序列


<400>  5
Glu Thr Asn Gly Glu Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp 
1               5                   10                  15      
Phe Ala Thr Val Arg Lys Val Leu Ser Met Pro Gln Val Asn Ile Val 
            20                  25                  30          
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Arg 
        35                  40                  45              
Pro Lys Arg Asn Ser Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp 
    50                  55                  60                  
Pro Lys Lys Tyr Gly Gly Phe Leu Trp Pro Thr Val Ala Tyr Ser Val 
65                  70                  75                  80  
Leu Val Val Ala Lys Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser 
                85                  90                  95      
Val Lys Glu Leu Leu Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu 
            100                 105                 110         
Lys Asn Pro Ile Asp Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys 
        115                 120                 125             
Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    130                 135                 140                 
Asn Gly Arg Lys Arg Met Leu Ala Ser Ala Lys Gln Leu Gln Lys Gly 
145                 150                 155                 160 
Asn Glu Leu Ala Leu Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala 
                165                 170                 175     
Ser His Tyr Glu Lys Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys 
            180                 185                 190         
Gln Leu Phe Val Glu Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu 
        195                 200                 205             
Gln Ile Ser Glu Phe Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu 
    210                 215                 220                 
Asp Lys Val Leu Ser Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg 
225                 230                 235                 240 
Glu Gln Ala Glu Asn Ile Ile His Leu Phe Thr Leu Thr Arg Leu Gly 
                245                 250                 255     
Ala Pro Arg Ala Phe Lys Tyr Phe Asp Thr Thr Ile Asp Pro Lys Gln 
            260                 265                 270         
Tyr Arg Ser Thr Lys Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser 
        275                 280                 285             
Ile Thr Gly Leu Tyr Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly 
    290                 295                 300                 
Asp 
305 

<210>  6
<211>  915
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  编码所述SpRY(D10A)蛋白C端片段的核苷酸序列


<400>  6
gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg        60
cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc       120
ggcttcagca aagagtctat cagacccaag aggaacagcg ataagctgat cgccagaaag       180
aaggactggg accctaagaa gtacggcggc ttcctgtggc ccaccgtggc ctattctgtg       240
ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg       300
ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa       360
gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg       420
ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccaagcagct gcagaaggga       480
aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag       540
aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag       600
cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc       660
gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga       720
gagcaggccg agaatatcat ccacctgttt accctgacca gactgggagc ccctagagcc       780
ttcaagtact ttgacaccac catcgacccc aagcagtaca gaagcaccaa agaggtgctg       840
gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct       900
cagctgggag gtgac                                                        915

<210>  7
<211>  32
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  融合蛋白CE-8e-SpRY含有的连接肽序列


<400>  7
Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr 
1               5                   10                  15      
Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser 
            20                  25                  30          

<210>  8
<211>  96
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  编码CE-8e-SpRY连接肽的核苷酸序列


<400>  8
tctggaggat ctagcggtgg ttcctctgga agcgagacac caggcacaag cgagtccgcc        60
acaccagaga gctccggcgg ctcctccgga ggatcc                                  96

<210>  9
<211>  18
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  融合蛋白CE-8e-SpRY两端的核定位信号的氨基酸序(bpNLS)


<400>  9
Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys Arg 
1               5                   10                  15      
Lys Val 
        

<210>  10
<211>  54
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  融合蛋白CE-8e-SpRY两端的核定位信号的核苷酸序列(bpNLS)


<400>  10
aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtc              54

<210>  11
<211>  7
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  核定位信号片段的氨基酸序列(SV40NLS)


<400>  11
Pro Lys Lys Lys Arg Lys Val 
1               5           

<210>  12
<211>  21
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  核定位信号的核苷酸序列(SV40NLS)


<400>  12
ccaaagaaga agcggaaagt c                                                  21

<210>  13
<211>  1581
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  CE-8e-SpRY融合蛋白的氨基酸序列


<400>  13
Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val Gly 
1               5                   10                  15      
Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys 
            20                  25                  30          
Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly 
        35                  40                  45              
Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Arg Thr Arg Leu Lys 
    50                  55                  60                  
Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr 
65                  70                  75                  80  
Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe 
                85                  90                  95      
Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His 
            100                 105                 110         
Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His 
        115                 120                 125             
Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser 
    130                 135                 140                 
Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met 
145                 150                 155                 160 
Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp 
                165                 170                 175     
Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn 
            180                 185                 190         
Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys 
        195                 200                 205             
Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu 
    210                 215                 220                 
Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu 
225                 230                 235                 240 
Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp 
                245                 250                 255     
Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp 
            260                 265                 270         
Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu 
        275                 280                 285             
Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile 
    290                 295                 300                 
Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met 
305                 310                 315                 320 
Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala 
                325                 330                 335     
Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp 
            340                 345                 350         
Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln 
        355                 360                 365             
Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly 
    370                 375                 380                 
Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys 
385                 390                 395                 400 
Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly 
                405                 410                 415     
Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu 
            420                 425                 430         
Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro 
        435                 440                 445             
Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met 
    450                 455                 460                 
Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val 
465                 470                 475                 480 
Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn 
                485                 490                 495     
Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu 
            500                 505                 510         
Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr 
        515                 520                 525             
Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys 
    530                 535                 540                 
Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     
Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala His 
625                 630                 635                 640 
Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe Lys 
    690                 695                 700                 
Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu His 
705                 710                 715                 720 
Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly Arg 
            740                 745                 750         
His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             
Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu 
    770                 775                 780                 
Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 
Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     
Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu 
            820                 825                 830         
Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys Asp 
        835                 840                 845             
Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 
Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn 
865                 870                 875                 880 
Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     
Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys 
            900                 905                 910         
Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             
His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu 
    930                 935                 940                 
Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 
Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu 
                965                 970                 975     
Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         
Gly Thr Ala Leu Ile Lys Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val 
        995                 1000                 1005             
Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys Ser 
    1010                 1015                 1020                 
Glu  Gln Glu Ile Gly Lys  Ala Thr Ala Lys Tyr  Phe Phe Tyr Ser Asn  
1025                 1030                 1035                 1040 
Ile Met Asn Phe Phe  Lys Ser Gly Gly Ser  Ser Gly Gly Ser Ser  Gly 
                1045                 1050                 1055     
Ser Glu Thr Pro  Gly Thr Ser Glu Ser  Ala Thr Pro Glu Ser  Ser Gly 
            1060                 1065                 1070         
Gly Ser Ser  Gly Gly Ser Ser Glu  Val Glu Phe Ser His  Glu Tyr Trp 
        1075                 1080                 1085             
Met Arg  His Ala Leu Thr Leu  Ala Lys Arg Ala Arg  Asp Glu Arg Glu 
    1090                 1095                 1100                 
Val  Pro Val Gly Ala Val  Leu Val Leu Asn Asn  Arg Val Ile Gly Glu  
1105                 1110                 1115                 1120 
Gly Trp Asn Arg Ala  Ile Gly Leu His Asp  Pro Thr Ala His Ala  Glu 
                1125                 1130                 1135     
Ile Met Ala Leu  Arg Gln Gly Gly Leu  Val Met Gln Asn Tyr  Arg Leu 
            1140                 1145                 1150         
Ile Asp Ala  Thr Leu Tyr Val Thr  Phe Glu Pro Cys Val  Met Cys Ala 
        1155                 1160                 1165             
Gly Ala  Met Ile His Ser Arg  Ile Gly Arg Val Val  Phe Gly Val Arg 
    1170                 1175                 1180                 
Asn  Ser Lys Arg Gly Ala  Ala Gly Ser Leu Met  Asn Val Leu Asn Tyr  
1185                 1190                 1195                 1200 
Pro Gly Met Asn His  Arg Val Glu Ile Thr  Glu Gly Ile Leu Ala  Asp 
                1205                 1210                 1215     
Glu Cys Ala Ala  Leu Leu Cys Asp Phe  Tyr Arg Met Pro Arg  Gln Val 
            1220                 1225                 1230         
Phe Asn Ala  Gln Lys Lys Ala Gln  Ser Ser Ile Asn Ser  Gly Gly Ser 
        1235                 1240                 1245             
Ser Gly  Gly Ser Ser Gly Ser  Glu Thr Pro Gly Thr  Ser Glu Ser Ala 
    1250                 1255                 1260                 
Thr  Pro Glu Ser Ser Gly  Gly Ser Ser Gly Gly  Ser Glu Thr Asn Gly  
1265                 1270                 1275                 1280 
Glu Thr Gly Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe Ala Thr  Val 
                1285                 1290                 1295     
Arg Lys Val Leu  Ser Met Pro Gln Val  Asn Ile Val Lys Lys  Thr Glu 
            1300                 1305                 1310         
Val Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Arg Pro  Lys Arg Asn 
        1315                 1320                 1325             
Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp Trp Asp  Pro Lys Lys Tyr 
    1330                 1335                 1340                 
Gly  Gly Phe Leu Trp Pro  Thr Val Ala Tyr Ser  Val Leu Val Val Ala  
1345                 1350                 1355                 1360 
Lys Val Glu Lys Gly  Lys Ser Lys Lys Leu  Lys Ser Val Lys Glu  Leu 
                1365                 1370                 1375     
Leu Gly Ile Thr  Ile Met Glu Arg Ser  Ser Phe Glu Lys Asn  Pro Ile 
            1380                 1385                 1390         
Asp Phe Leu  Glu Ala Lys Gly Tyr  Lys Glu Val Lys Lys  Asp Leu Ile 
        1395                 1400                 1405             
Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Glu Leu Glu  Asn Gly Arg Lys 
    1410                 1415                 1420                 
Arg  Met Leu Ala Ser Ala  Lys Gln Leu Gln Lys  Gly Asn Glu Leu Ala  
1425                 1430                 1435                 1440 
Leu Pro Ser Lys Tyr  Val Asn Phe Leu Tyr  Leu Ala Ser His Tyr  Glu 
                1445                 1450                 1455     
Lys Leu Lys Gly  Ser Pro Glu Asp Asn  Glu Gln Lys Gln Leu  Phe Val 
            1460                 1465                 1470         
Glu Gln His  Lys His Tyr Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu 
        1475                 1480                 1485             
Phe Ser  Lys Arg Val Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val Leu 
    1490                 1495                 1500                 
Ser  Ala Tyr Asn Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln Ala Glu  
1505                 1510                 1515                 1520 
Asn Ile Ile His Leu  Phe Thr Leu Thr Arg  Leu Gly Ala Pro Arg  Ala 
                1525                 1530                 1535     
Phe Lys Tyr Phe  Asp Thr Thr Ile Asp  Pro Lys Gln Tyr Arg  Ser Thr 
            1540                 1545                 1550         
Lys Glu Val  Leu Asp Ala Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu 
        1555                 1560                 1565             
Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu Gly Gly  Asp 
    1570                 1575                 1580     

<210>  14
<211>  4743
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  CE-8e-SpRY融合蛋白的核苷酸序列


<400>  14
gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc        60
accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac       120
agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgagaga       180
acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat       240
ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg       300
gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac       360
atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa       420
ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg       480
atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg       540
gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc       600
aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg       660
ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg       720
attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat       780
gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag       840
atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg       900
ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg       960
atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag      1020
cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc      1080
tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa      1140
aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag      1200
cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc      1260
attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag      1320
aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga      1380
ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg      1440
gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac      1500
ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat      1560
aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc      1620
ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg      1680
aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc      1740
ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc      1800
aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg      1860
accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac      1920
ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg      1980
ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat      2040
ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc      2100
ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac      2160
gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg      2220
aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc      2280
gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg      2340
aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg      2400
gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat      2460
atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc      2520
gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac      2580
aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac      2640
tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc      2700
aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg      2760
gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact      2820
aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag      2880
ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac      2940
caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac      3000
cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg      3060
atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac      3120
atcatgaact ttttcaagtc tggaggatct agcggtggtt cctctggaag cgagacacca      3180
ggcacaagcg agtccgccac accagagagc tccggcggct cctccggagg atcctctgag      3240
gtggagtttt cccacgagta ctggatgaga catgccctga ccctggccaa gagggcacgg      3300
gatgagaggg aggtgcctgt gggagccgtg ctggtgctga acaatagagt gatcggcgag      3360
ggctggaaca gagccatcgg cctgcacgac ccaacagccc atgccgaaat tatggccctg      3420
agacagggcg gcctggtcat gcagaactac agactgattg acgccaccct gtacgtgaca      3480
ttcgagcctt gcgtgatgtg cgccggcgcc atgatccact ctaggatcgg ccgcgtggtg      3540
tttggcgtga ggaactcaaa aagaggcgcc gcaggctccc tgatgaacgt gctgaactac      3600
cccggcatga atcaccgcgt cgaaattacc gagggaatcc tggcagatga atgtgccgcc      3660
ctgctgtgcg atttctatcg gatgcctaga caggtgttca atgctcagaa gaaggcccag      3720
agctccatca actccggagg atctagcgga ggctcctctg gctctgagac acctggcaca      3780
agcgagagcg caacacctga aagcagcggg ggcagcagcg gggggtcaga gacaaacggc      3840
gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg      3900
agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa      3960
gagtctatca gacccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac      4020
cctaagaagt acggcggctt cctgtggccc accgtggcct attctgtgct ggtggtggcc      4080
aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc      4140
atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac      4200
aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa      4260
aacggccgga agagaatgct ggcctctgcc aagcagctgc agaagggaaa cgaactggcc      4320
ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc      4380
tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac      4440
gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg      4500
gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag      4560
aatatcatcc acctgtttac cctgaccaga ctgggagccc ctagagcctt caagtacttt      4620
gacaccacca tcgaccccaa gcagtacaga agcaccaaag aggtgctgga cgccaccctg      4680
atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggt      4740
gac                                                                    4743

<210>  15
<211>  498
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  TadA8e V106W核苷酸序列


<400>  15
tctgaggtgg agttttccca cgagtactgg atgagacatg ccctgaccct ggccaagagg        60
gcacgggatg agagggaggt gcctgtggga gccgtgctgg tgctgaacaa tagagtgatc       120
ggcgagggct ggaacagagc catcggcctg cacgacccaa cagcccatgc cgaaattatg       180
gccctgagac agggcggcct ggtcatgcag aactacagac tgattgacgc caccctgtac       240
gtgacattcg agccttgcgt gatgtgcgcc ggcgccatga tccactctag gatcggccgc       300
gtggtgtttg gctggaggaa ctcaaaaaga ggcgccgcag gctccctgat gaacgtgctg       360
aactaccccg gcatgaatca ccgcgtcgaa attaccgagg gaatcctggc agatgaatgt       420
gccgccctgc tgtgcgattt ctatcggatg cctagacagg tgttcaatgc tcagaagaag       480
gcccagagct ccatcaac                                                     498

<210>  16
<211>  4101
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  SpRY D10A核苷酸序列


<400>  16
gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc        60
accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac       120
agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgagaga       180
acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat       240
ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg       300
gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac       360
atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa       420
ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg       480
atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg       540
gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc       600
aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg       660
ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg       720
attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat       780
gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag       840
atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg       900
ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg       960
atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag      1020
cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc      1080
tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa      1140
aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag      1200
cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc      1260
attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag      1320
aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga      1380
ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg      1440
gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac      1500
ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat      1560
aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc      1620
ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg      1680
aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc      1740
ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc      1800
aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg      1860
accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac      1920
ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg      1980
ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat      2040
ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc      2100
ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac      2160
gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg      2220
aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc      2280
gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg      2340
aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg      2400
gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat      2460
atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc      2520
gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac      2580
aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac      2640
tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc      2700
aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg      2760
gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact      2820
aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag      2880
ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac      2940
caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac      3000
cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg      3060
atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac      3120
atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct      3180
ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc      3240
accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag      3300
acaggcggct tcagcaaaga gtctatcaga cccaagagga acagcgataa gctgatcgcc      3360
agaaagaagg actgggaccc taagaagtac ggcggcttcc tgtggcccac cgtggcctat      3420
tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa      3480
gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt      3540
ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac      3600
tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccaa gcagctgcag      3660
aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac      3720
tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag      3780
cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc      3840
ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc      3900
atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccagact gggagcccct      3960
agagccttca agtactttga caccaccatc gaccccaagc agtacagaag caccaaagag      4020
gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac      4080
ctgtctcagc tgggaggtga c                                                4101

<210>  17
<211>  4101
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  SpRY D10A-HF核苷酸序列


<400>  17
gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc        60
accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac       120
agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgagaga       180
acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat       240
ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg       300
gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac       360
atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa       420
ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg       480
atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg       540
gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc       600
aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg       660
ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg       720
attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat       780
gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag       840
atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg       900
ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg       960
atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag      1020
cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc      1080
tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa      1140
aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag      1200
cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc      1260
attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag      1320
aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga      1380
ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg      1440
gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccgcctt cgataagaac      1500
ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat      1560
aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc      1620
ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg      1680
aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc      1740
ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc      1800
aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg      1860
accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac      1920
ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcgcg      1980
ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat      2040
ttcctgaagt ccgacggctt cgccaacaga aacttcatgg cgctgatcca cgacgacagc      2100
ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac      2160
gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg      2220
aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc      2280
gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg      2340
aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg      2400
gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat      2460
atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc      2520
gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac      2580
aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac      2640
tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc      2700
aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg      2760
gtggaaaccc gggcgatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact      2820
aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag      2880
ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac      2940
caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac      3000
cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg      3060
atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac      3120
atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct      3180
ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc      3240
accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag      3300
acaggcggct tcagcaaaga gtctatcaga cccaagagga acagcgataa gctgatcgcc      3360
agaaagaagg actgggaccc taagaagtac ggcggcttcc tgtggcccac cgtggcctat      3420
tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa      3480
gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt      3540
ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac      3600
tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccaa gcagctgcag      3660
aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac      3720
tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag      3780
cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc      3840
ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc      3900
atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccagact gggagcccct      3960
agagccttca agtactttga caccaccatc gaccccaagc agtacagaag caccaaagag      4020
gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac      4080
ctgtctcagc tgggaggtga c                                                4101

<210>  18
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA1


<400>  18
cctgcttgca agtgtcaacc                                                    20

<210>  19
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA2


<400>  19
tacttgcgac agttccttcc                                                    20

<210>  20
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA3


<400>  20
catttccctc tacgctcgct                                                    20

<210>  21
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA4


<400>  21
gtgaaacacc acgcccggcc                                                    20

<210>  22
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA5


<400>  22
cctcttctgg aaaggggtac                                                    20

<210>  23
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA6


<400>  23
gacagttcct tccaattcca                                                    20

<210>  24
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA7


<400>  24
aacaacagta cttgcgacag                                                    20

<210>  25
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA8


<400>  25
gcagcagcct ggaaaagtac                                                    20

<210>  26
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA9


<400>  26
gatgcagcga atgtgaaatc                                                    20

<210>  27
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA10


<400>  27
tcatcttagg ccttcaagga                                                    20

<210>  28
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA11	


<400>  28
gagatgcagc gaatgtgaaa                                                    20

<210>  29
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA12


<400>  29
gagcagggaa gaaggaatca                                                    20

<210>  30
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA13


<400>  30
gaaaagtact tggggaccaa                                                    20

<210>  31
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA14


<400>  31
ttggaactcc tgcttgcaag                                                    20

<210>  32
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA15


<400>  32
aaggccaagc ttgcctgccc                                                    20

<210>  33
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA16


<400>  33
aaagctgcat ccaccccccg                                                    20

<210>  34
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA17


<400>  34
ctgctagacg acagcgcagg                                                    20

<210>  35
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA18


<400>  35
gtaacctccc ttgaaagggg                                                    20

<210>  36
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA19


<400>  36
ggagcatctg agtccagggg                                                    20

<210>  37
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA20


<400>  37
ctagacgaca gcgcaggcaa                                                    20

<210>  38
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA21


<400>  38
ccgaatgcaa aggttctctg                                                    20

<210>  39
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA22


<400>  39
ccgtccaaag tcaaattgct                                                    20

<210>  40
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA23


<400>  40
tggcacatag atagtaagtg                                                    20

<210>  41
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA24


<400>  41
tggccgaatg caaaggttct                                                    20

<210>  42
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA26


<400>  42
tagactaatt atataagcct                                                    20

<210>  43
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA26


<400>  43
tggctaagca tcaggagttt                                                    20

<210>  44
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA27


<400>  44
gagttccacc cgctgtgcgt                                                    20

<210>  45
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA28


<400>  45
atgcaaaggt tctctgctag                                                    20

<210>  46
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA29


<400>  46
gaatgcaaag gttctctgct                                                    20

<210>  47
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA30


<400>  47
tcggccaatg gggcacaagg                                                    20

<210>  48
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA31


<400>  48
gttacaccaa agggctagaa                                                    20

<210>  49
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA32


<400>  49
ggttacacca aagggctaga                                                    20

<210>  50
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA33


<400>  50
taccaacaat agaggcccat                                                    20

<210>  51
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA34


<400>  51
ctgtcaaact gtgcgtatga                                                    20

<210>  52
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA35


<400>  52
accaacaata gaggcccatt                                                    20

<210>  53
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA36


<400>  53
gtttacataa aagatcttca                                                    20

<210>  54
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA37


<400>  54
ctaccaacaa tagaggccca                                                    20

<210>  55
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA38


<400>  55
acatcatcag atattctgca                                                    20

<210>  56
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA39


<400>  56
gctgcaaaca agtgcagaat                                                    20

<210>  57
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA40


<400>  57
gtctcagagg atgtgttgtt                                                    20

<210>  58
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA41


<400>  58
ctgcagccca agcctcagtg                                                    20

<210>  59
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA42


<400>  59
tcctaaacca gtgtcaggga                                                    20

<210>  60
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA43


<400>  60
aaagatcttc acaggctacc                                                    20

<210>  61
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA44


<400>  61
ccaacaatag aggcccatta                                                    20

<210>  62
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA45


<400>  62
aggtcctaaa ccagtgtcag                                                    20

<210>  63
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA46


<400>  63
aatacaaata gttaagaaca                                                    20

<210>  64
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA47


<400>  64
ctggaacaca aagcatagac                                                    20

<210>  65
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sgRNA48


<400>  65
gaacacaaag catagactgc                                                    20

<210>  66
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  用于RNA脱靶检测的sgRNA序列


<400>  66
ctggaacaca aagcatagac                                                    20

<210>  67
<211>  1803
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  ABEmax-SpRY融合蛋白的氨基酸序列


<400>  67
Met Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys 
1               5                   10                  15      
Arg Lys Val Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His 
            20                  25                  30          
Ala Leu Thr Leu Ala Lys Arg Ala Trp Asp Glu Arg Glu Val Pro Val 
        35                  40                  45              
Gly Ala Val Leu Val His Asn Asn Arg Val Ile Gly Glu Gly Trp Asn 
    50                  55                  60                  
Arg Pro Ile Gly Arg His Asp Pro Thr Ala His Ala Glu Ile Met Ala 
65                  70                  75                  80  
Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala 
                85                  90                  95      
Thr Leu Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Met 
            100                 105                 110         
Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Ala Arg Asp Ala Lys 
        115                 120                 125             
Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met 
    130                 135                 140                 
Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala 
145                 150                 155                 160 
Ala Leu Leu Ser Asp Phe Phe Arg Met Arg Arg Gln Glu Ile Lys Ala 
                165                 170                 175     
Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly 
            180                 185                 190         
Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
        195                 200                 205             
Ser Ser Gly Gly Ser Ser Gly Gly Ser Ser Glu Val Glu Phe Ser His 
    210                 215                 220                 
Glu Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp 
225                 230                 235                 240 
Glu Arg Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val 
                245                 250                 255     
Ile Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala 
            260                 265                 270         
His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn 
        275                 280                 285             
Tyr Arg Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys Val 
    290                 295                 300                 
Met Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe 
305                 310                 315                 320 
Gly Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val 
                325                 330                 335     
Leu His Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile 
            340                 345                 350         
Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met Pro 
        355                 360                 365             
Arg Gln Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser 
    370                 375                 380                 
Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser 
385                 390                 395                 400 
Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Asp 
                405                 410                 415     
Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val Gly Trp 
            420                 425                 430         
Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys Val 
        435                 440                 445             
Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly Ala 
    450                 455                 460                 
Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Arg Thr Arg Leu Lys Arg 
465                 470                 475                 480 
Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu 
                485                 490                 495     
Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe Phe 
            500                 505                 510         
His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His Glu 
        515                 520                 525             
Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His Glu 
    530                 535                 540                 
Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser Thr 
545                 550                 555                 560 
Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met Ile 
                565                 570                 575     
Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn 
            580                 585                 590         
Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln 
        595                 600                 605             
Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys Ala 
    610                 615                 620                 
Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile 
625                 630                 635                 640 
Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile 
                645                 650                 655     
Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu 
            660                 665                 670         
Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp 
        675                 680                 685             
Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe 
    690                 695                 700                 
Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu 
705                 710                 715                 720 
Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met Ile 
                725                 730                 735     
Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala Leu 
            740                 745                 750         
Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln 
        755                 760                 765             
Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu 
    770                 775                 780                 
Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly Thr 
785                 790                 795                 800 
Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln 
                805                 810                 815     
Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly Glu 
            820                 825                 830         
Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys 
        835                 840                 845             
Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr 
    850                 855                 860                 
Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met Thr 
865                 870                 875                 880 
Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val 
                885                 890                 895     
Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe 
            900                 905                 910         
Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu 
        915                 920                 925             
Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val 
    930                 935                 940                 
Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys 
945                 950                 955                 960 
Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val Lys 
                965                 970                 975     
Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser Val 
            980                 985                 990         
Glu Ile Ser Gly Val Glu Asp Arg  Phe Asn Ala Ser Leu  Gly Thr Tyr 
        995                 1000                 1005             
His Asp  Leu Leu Lys Ile Ile  Lys Asp Lys Asp Phe  Leu Asp Asn Glu 
    1010                 1015                 1020                 
Glu  Asn Glu Asp Ile Leu  Glu Asp Ile Val Leu  Thr Leu Thr Leu Phe  
1025                 1030                 1035                 1040 
Glu Asp Arg Glu Met  Ile Glu Glu Arg Leu  Lys Thr Tyr Ala His  Leu 
                1045                 1050                 1055     
Phe Asp Asp Lys  Val Met Lys Gln Leu  Lys Arg Arg Arg Tyr  Thr Gly 
            1060                 1065                 1070         
Trp Gly Arg  Leu Ser Arg Lys Leu  Ile Asn Gly Ile Arg  Asp Lys Gln 
        1075                 1080                 1085             
Ser Gly  Lys Thr Ile Leu Asp  Phe Leu Lys Ser Asp  Gly Phe Ala Asn 
    1090                 1095                 1100                 
Arg  Asn Phe Met Gln Leu  Ile His Asp Asp Ser  Leu Thr Phe Lys Glu  
1105                 1110                 1115                 1120 
Asp Ile Gln Lys Ala  Gln Val Ser Gly Gln  Gly Asp Ser Leu His  Glu 
                1125                 1130                 1135     
His Ile Ala Asn  Leu Ala Gly Ser Pro  Ala Ile Lys Lys Gly  Ile Leu 
            1140                 1145                 1150         
Gln Thr Val  Lys Val Val Asp Glu  Leu Val Lys Val Met  Gly Arg His 
        1155                 1160                 1165             
Lys Pro  Glu Asn Ile Val Ile  Glu Met Ala Arg Glu  Asn Gln Thr Thr 
    1170                 1175                 1180                 
Gln  Lys Gly Gln Lys Asn  Ser Arg Glu Arg Met  Lys Arg Ile Glu Glu  
1185                 1190                 1195                 1200 
Gly Ile Lys Glu Leu  Gly Ser Gln Ile Leu  Lys Glu His Pro Val  Glu 
                1205                 1210                 1215     
Asn Thr Gln Leu  Gln Asn Glu Lys Leu  Tyr Leu Tyr Tyr Leu  Gln Asn 
            1220                 1225                 1230         
Gly Arg Asp  Met Tyr Val Asp Gln  Glu Leu Asp Ile Asn  Arg Leu Ser 
        1235                 1240                 1245             
Asp Tyr  Asp Val Asp His Ile  Val Pro Gln Ser Phe  Leu Lys Asp Asp 
    1250                 1255                 1260                 
Ser  Ile Asp Asn Lys Val  Leu Thr Arg Ser Asp  Lys Asn Arg Gly Lys  
1265                 1270                 1275                 1280 
Ser Asp Asn Val Pro  Ser Glu Glu Val Val  Lys Lys Met Lys Asn  Tyr 
                1285                 1290                 1295     
Trp Arg Gln Leu  Leu Asn Ala Lys Leu  Ile Thr Gln Arg Lys  Phe Asp 
            1300                 1305                 1310         
Asn Leu Thr  Lys Ala Glu Arg Gly  Gly Leu Ser Glu Leu  Asp Lys Ala 
        1315                 1320                 1325             
Gly Phe  Ile Lys Arg Gln Leu  Val Glu Thr Arg Gln  Ile Thr Lys His 
    1330                 1335                 1340                 
Val  Ala Gln Ile Leu Asp  Ser Arg Met Asn Thr  Lys Tyr Asp Glu Asn  
1345                 1350                 1355                 1360 
Asp Lys Leu Ile Arg  Glu Val Lys Val Ile  Thr Leu Lys Ser Lys  Leu 
                1365                 1370                 1375     
Val Ser Asp Phe  Arg Lys Asp Phe Gln  Phe Tyr Lys Val Arg  Glu Ile 
            1380                 1385                 1390         
Asn Asn Tyr  His His Ala His Asp  Ala Tyr Leu Asn Ala  Val Val Gly 
        1395                 1400                 1405             
Thr Ala  Leu Ile Lys Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val Tyr 
    1410                 1415                 1420                 
Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys Ser Glu  
1425                 1430                 1435                 1440 
Gln Glu Ile Gly Lys  Ala Thr Ala Lys Tyr  Phe Phe Tyr Ser Asn  Ile 
                1445                 1450                 1455     
Met Asn Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn Gly Glu  Ile Arg 
            1460                 1465                 1470         
Lys Arg Pro  Leu Ile Glu Thr Asn  Gly Glu Thr Gly Glu  Ile Val Trp 
        1475                 1480                 1485             
Asp Lys  Gly Arg Asp Phe Ala  Thr Val Arg Lys Val  Leu Ser Met Pro 
    1490                 1495                 1500                 
Gln  Val Asn Ile Val Lys  Lys Thr Glu Val Gln  Thr Gly Gly Phe Ser  
1505                 1510                 1515                 1520 
Lys Glu Ser Ile Arg  Pro Lys Arg Asn Ser  Asp Lys Leu Ile Ala  Arg 
                1525                 1530                 1535     
Lys Lys Asp Trp  Asp Pro Lys Lys Tyr  Gly Gly Phe Leu Trp  Pro Thr 
            1540                 1545                 1550         
Val Ala Tyr  Ser Val Leu Val Val  Ala Lys Val Glu Lys  Gly Lys Ser 
        1555                 1560                 1565             
Lys Lys  Leu Lys Ser Val Lys  Glu Leu Leu Gly Ile  Thr Ile Met Glu 
    1570                 1575                 1580                 
Arg  Ser Ser Phe Glu Lys  Asn Pro Ile Asp Phe  Leu Glu Ala Lys Gly  
1585                 1590                 1595                 1600 
Tyr Lys Glu Val Lys  Lys Asp Leu Ile Ile  Lys Leu Pro Lys Tyr  Ser 
                1605                 1610                 1615     
Leu Phe Glu Leu  Glu Asn Gly Arg Lys  Arg Met Leu Ala Ser  Ala Lys 
            1620                 1625                 1630         
Gln Leu Gln  Lys Gly Asn Glu Leu  Ala Leu Pro Ser Lys  Tyr Val Asn 
        1635                 1640                 1645             
Phe Leu  Tyr Leu Ala Ser His  Tyr Glu Lys Leu Lys  Gly Ser Pro Glu 
    1650                 1655                 1660                 
Asp  Asn Glu Gln Lys Gln  Leu Phe Val Glu Gln  His Lys His Tyr Leu  
1665                 1670                 1675                 1680 
Asp Glu Ile Ile Glu  Gln Ile Ser Glu Phe  Ser Lys Arg Val Ile  Leu 
                1685                 1690                 1695     
Ala Asp Ala Asn  Leu Asp Lys Val Leu  Ser Ala Tyr Asn Lys  His Arg 
            1700                 1705                 1710         
Asp Lys Pro  Ile Arg Glu Gln Ala  Glu Asn Ile Ile His  Leu Phe Thr 
        1715                 1720                 1725             
Leu Thr  Arg Leu Gly Ala Pro  Arg Ala Phe Lys Tyr  Phe Asp Thr Thr 
    1730                 1735                 1740                 
Ile  Asp Pro Lys Gln Tyr  Arg Ser Thr Lys Glu  Val Leu Asp Ala Thr  
1745                 1750                 1755                 1760 
Leu Ile His Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Arg Ile Asp  Leu 
                1765                 1770                 1775     
Ser Gln Leu Gly  Gly Asp Ser Gly Gly  Ser Lys Arg Thr Ala  Asp Gly 
            1780                 1785                 1790         
Ser Glu Phe  Glu Pro Lys Lys Lys  Arg Lys Val 
        1795                 1800             

<210>  68
<211>  1605
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  8e-SpRY融合蛋白的氨基酸序列


<400>  68
Met Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys 
1               5                   10                  15      
Arg Lys Val Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His 
            20                  25                  30          
Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val 
        35                  40                  45              
Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn 
    50                  55                  60                  
Arg Ala Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala 
65                  70                  75                  80  
Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala 
                85                  90                  95      
Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met 
            100                 105                 110         
Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ser Lys 
        115                 120                 125             
Arg Gly Ala Ala Gly Ser Leu Met Asn Val Leu Asn Tyr Pro Gly Met 
    130                 135                 140                 
Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala 
145                 150                 155                 160 
Ala Leu Leu Cys Asp Phe Tyr Arg Met Pro Arg Gln Val Phe Asn Ala 
                165                 170                 175     
Gln Lys Lys Ala Gln Ser Ser Ile Asn Ser Gly Gly Ser Ser Gly Gly 
            180                 185                 190         
Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
        195                 200                 205             
Ser Ser Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly 
    210                 215                 220                 
Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu 
225                 230                 235                 240 
Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg 
                245                 250                 255     
His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly 
            260                 265                 270         
Glu Thr Ala Glu Arg Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr 
        275                 280                 285             
Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn 
    290                 295                 300                 
Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser 
305                 310                 315                 320 
Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly 
                325                 330                 335     
Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr 
            340                 345                 350         
His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg 
        355                 360                 365             
Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe 
    370                 375                 380                 
Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu 
385                 390                 395                 400 
Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro 
                405                 410                 415     
Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu 
            420                 425                 430         
Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu 
        435                 440                 445             
Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu 
    450                 455                 460                 
Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu 
465                 470                 475                 480 
Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala 
                485                 490                 495     
Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu 
            500                 505                 510         
Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile 
        515                 520                 525             
Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His 
    530                 535                 540                 
His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro 
545                 550                 555                 560 
Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala 
                565                 570                 575     
Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile 
            580                 585                 590         
Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys 
        595                 600                 605             
Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly 
    610                 615                 620                 
Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg 
625                 630                 635                 640 
Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile 
                645                 650                 655     
Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala 
            660                 665                 670         
Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr 
        675                 680                 685             
Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala 
    690                 695                 700                 
Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn 
705                 710                 715                 720 
Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val 
                725                 730                 735     
Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys 
            740                 745                 750         
Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu 
        755                 760                 765             
Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr 
    770                 775                 780                 
Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu 
785                 790                 795                 800 
Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile 
                805                 810                 815     
Ile Lys Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu 
            820                 825                 830         
Glu Asp Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile 
        835                 840                 845             
Glu Glu Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met 
    850                 855                 860                 
Lys Gln Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg 
865                 870                 875                 880 
Lys Leu Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu 
                885                 890                 895     
Asp Phe Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu 
            900                 905                 910         
Ile His Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln 
        915                 920                 925             
Val Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala 
    930                 935                 940                 
Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val 
945                 950                 955                 960 
Asp Glu Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val 
                965                 970                 975     
Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn 
            980                 985                 990         
Ser Arg Glu Arg Met Lys Arg Ile  Glu Glu Gly Ile Lys  Glu Leu Gly 
        995                 1000                 1005             
Ser Gln  Ile Leu Lys Glu His  Pro Val Glu Asn Thr  Gln Leu Gln Asn 
    1010                 1015                 1020                 
Glu  Lys Leu Tyr Leu Tyr  Tyr Leu Gln Asn Gly  Arg Asp Met Tyr Val  
1025                 1030                 1035                 1040 
Asp Gln Glu Leu Asp  Ile Asn Arg Leu Ser  Asp Tyr Asp Val Asp  His 
                1045                 1050                 1055     
Ile Val Pro Gln  Ser Phe Leu Lys Asp  Asp Ser Ile Asp Asn  Lys Val 
            1060                 1065                 1070         
Leu Thr Arg  Ser Asp Lys Asn Arg  Gly Lys Ser Asp Asn  Val Pro Ser 
        1075                 1080                 1085             
Glu Glu  Val Val Lys Lys Met  Lys Asn Tyr Trp Arg  Gln Leu Leu Asn 
    1090                 1095                 1100                 
Ala  Lys Leu Ile Thr Gln  Arg Lys Phe Asp Asn  Leu Thr Lys Ala Glu  
1105                 1110                 1115                 1120 
Arg Gly Gly Leu Ser  Glu Leu Asp Lys Ala  Gly Phe Ile Lys Arg  Gln 
                1125                 1130                 1135     
Leu Val Glu Thr  Arg Gln Ile Thr Lys  His Val Ala Gln Ile  Leu Asp 
            1140                 1145                 1150         
Ser Arg Met  Asn Thr Lys Tyr Asp  Glu Asn Asp Lys Leu  Ile Arg Glu 
        1155                 1160                 1165             
Val Lys  Val Ile Thr Leu Lys  Ser Lys Leu Val Ser  Asp Phe Arg Lys 
    1170                 1175                 1180                 
Asp  Phe Gln Phe Tyr Lys  Val Arg Glu Ile Asn  Asn Tyr His His Ala  
1185                 1190                 1195                 1200 
His Asp Ala Tyr Leu  Asn Ala Val Val Gly  Thr Ala Leu Ile Lys  Lys 
                1205                 1210                 1215     
Tyr Pro Lys Leu  Glu Ser Glu Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr 
            1220                 1225                 1230         
Asp Val Arg  Lys Met Ile Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala 
        1235                 1240                 1245             
Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys Thr 
    1250                 1255                 1260                 
Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu Ile Glu  
1265                 1270                 1275                 1280 
Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly Arg Asp  Phe 
                1285                 1290                 1295     
Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val Asn Ile  Val Lys 
            1300                 1305                 1310         
Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys Glu Ser  Ile Arg Pro 
        1315                 1320                 1325             
Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg Lys Lys  Asp Trp Asp Pro 
    1330                 1335                 1340                 
Lys  Lys Tyr Gly Gly Phe  Leu Trp Pro Thr Val  Ala Tyr Ser Val Leu  
1345                 1350                 1355                 1360 
Val Val Ala Lys Val  Glu Lys Gly Lys Ser  Lys Lys Leu Lys Ser  Val 
                1365                 1370                 1375     
Lys Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser Ser Phe  Glu Lys 
            1380                 1385                 1390         
Asn Pro Ile  Asp Phe Leu Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys 
        1395                 1400                 1405             
Asp Leu  Ile Ile Lys Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn 
    1410                 1415                 1420                 
Gly  Arg Lys Arg Met Leu  Ala Ser Ala Lys Gln  Leu Gln Lys Gly Asn  
1425                 1430                 1435                 1440 
Glu Leu Ala Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser 
                1445                 1450                 1455     
His Tyr Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln 
            1460                 1465                 1470         
Leu Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
        1475                 1480                 1485             
Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Asp 
    1490                 1495                 1500                 
Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile Arg Glu  
1505                 1510                 1515                 1520 
Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Arg Leu Gly  Ala 
                1525                 1530                 1535     
Pro Arg Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp Pro Lys  Gln Tyr 
            1540                 1545                 1550         
Arg Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu Ile His  Gln Ser Ile 
        1555                 1560                 1565             
Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu Ser Gln  Leu Gly Gly Asp 
    1570                 1575                 1580                 
Ser  Gly Gly Ser Lys Arg  Thr Ala Asp Gly Ser  Glu Phe Glu Pro Lys  
1585                 1590                 1595                 1600 
Lys Lys Arg Lys Val  
                1605 

<210>  69
<211>  1605
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  V106W-SpRY融合蛋白的氨基酸序列


<400>  69
Met Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys 
1               5                   10                  15      
Arg Lys Val Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His 
            20                  25                  30          
Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val 
        35                  40                  45              
Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn 
    50                  55                  60                  
Arg Ala Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala 
65                  70                  75                  80  
Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala 
                85                  90                  95      
Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met 
            100                 105                 110         
Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Trp Arg Asn Ser Lys 
        115                 120                 125             
Arg Gly Ala Ala Gly Ser Leu Met Asn Val Leu Asn Tyr Pro Gly Met 
    130                 135                 140                 
Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala 
145                 150                 155                 160 
Ala Leu Leu Cys Asp Phe Tyr Arg Met Pro Arg Gln Val Phe Asn Ala 
                165                 170                 175     
Gln Lys Lys Ala Gln Ser Ser Ile Asn Ser Gly Gly Ser Ser Gly Gly 
            180                 185                 190         
Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
        195                 200                 205             
Ser Ser Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly 
    210                 215                 220                 
Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu 
225                 230                 235                 240 
Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg 
                245                 250                 255     
His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly 
            260                 265                 270         
Glu Thr Ala Glu Arg Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr 
        275                 280                 285             
Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn 
    290                 295                 300                 
Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser 
305                 310                 315                 320 
Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly 
                325                 330                 335     
Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr 
            340                 345                 350         
His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg 
        355                 360                 365             
Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe 
    370                 375                 380                 
Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu 
385                 390                 395                 400 
Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro 
                405                 410                 415     
Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu 
            420                 425                 430         
Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu 
        435                 440                 445             
Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu 
    450                 455                 460                 
Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu 
465                 470                 475                 480 
Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala 
                485                 490                 495     
Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu 
            500                 505                 510         
Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile 
        515                 520                 525             
Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His 
    530                 535                 540                 
His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro 
545                 550                 555                 560 
Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala 
                565                 570                 575     
Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile 
            580                 585                 590         
Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys 
        595                 600                 605             
Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly 
    610                 615                 620                 
Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg 
625                 630                 635                 640 
Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile 
                645                 650                 655     
Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala 
            660                 665                 670         
Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr 
        675                 680                 685             
Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala 
    690                 695                 700                 
Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn 
705                 710                 715                 720 
Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val 
                725                 730                 735     
Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys 
            740                 745                 750         
Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu 
        755                 760                 765             
Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr 
    770                 775                 780                 
Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu 
785                 790                 795                 800 
Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile 
                805                 810                 815     
Ile Lys Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu 
            820                 825                 830         
Glu Asp Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile 
        835                 840                 845             
Glu Glu Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met 
    850                 855                 860                 
Lys Gln Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg 
865                 870                 875                 880 
Lys Leu Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu 
                885                 890                 895     
Asp Phe Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu 
            900                 905                 910         
Ile His Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln 
        915                 920                 925             
Val Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala 
    930                 935                 940                 
Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val 
945                 950                 955                 960 
Asp Glu Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val 
                965                 970                 975     
Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn 
            980                 985                 990         
Ser Arg Glu Arg Met Lys Arg Ile  Glu Glu Gly Ile Lys  Glu Leu Gly 
        995                 1000                 1005             
Ser Gln  Ile Leu Lys Glu His  Pro Val Glu Asn Thr  Gln Leu Gln Asn 
    1010                 1015                 1020                 
Glu  Lys Leu Tyr Leu Tyr  Tyr Leu Gln Asn Gly  Arg Asp Met Tyr Val  
1025                 1030                 1035                 1040 
Asp Gln Glu Leu Asp  Ile Asn Arg Leu Ser  Asp Tyr Asp Val Asp  His 
                1045                 1050                 1055     
Ile Val Pro Gln  Ser Phe Leu Lys Asp  Asp Ser Ile Asp Asn  Lys Val 
            1060                 1065                 1070         
Leu Thr Arg  Ser Asp Lys Asn Arg  Gly Lys Ser Asp Asn  Val Pro Ser 
        1075                 1080                 1085             
Glu Glu  Val Val Lys Lys Met  Lys Asn Tyr Trp Arg  Gln Leu Leu Asn 
    1090                 1095                 1100                 
Ala  Lys Leu Ile Thr Gln  Arg Lys Phe Asp Asn  Leu Thr Lys Ala Glu  
1105                 1110                 1115                 1120 
Arg Gly Gly Leu Ser  Glu Leu Asp Lys Ala  Gly Phe Ile Lys Arg  Gln 
                1125                 1130                 1135     
Leu Val Glu Thr  Arg Gln Ile Thr Lys  His Val Ala Gln Ile  Leu Asp 
            1140                 1145                 1150         
Ser Arg Met  Asn Thr Lys Tyr Asp  Glu Asn Asp Lys Leu  Ile Arg Glu 
        1155                 1160                 1165             
Val Lys  Val Ile Thr Leu Lys  Ser Lys Leu Val Ser  Asp Phe Arg Lys 
    1170                 1175                 1180                 
Asp  Phe Gln Phe Tyr Lys  Val Arg Glu Ile Asn  Asn Tyr His His Ala  
1185                 1190                 1195                 1200 
His Asp Ala Tyr Leu  Asn Ala Val Val Gly  Thr Ala Leu Ile Lys  Lys 
                1205                 1210                 1215     
Tyr Pro Lys Leu  Glu Ser Glu Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr 
            1220                 1225                 1230         
Asp Val Arg  Lys Met Ile Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala 
        1235                 1240                 1245             
Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys Thr 
    1250                 1255                 1260                 
Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu Ile Glu  
1265                 1270                 1275                 1280 
Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly Arg Asp  Phe 
                1285                 1290                 1295     
Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val Asn Ile  Val Lys 
            1300                 1305                 1310         
Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys Glu Ser  Ile Arg Pro 
        1315                 1320                 1325             
Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg Lys Lys  Asp Trp Asp Pro 
    1330                 1335                 1340                 
Lys  Lys Tyr Gly Gly Phe  Leu Trp Pro Thr Val  Ala Tyr Ser Val Leu  
1345                 1350                 1355                 1360 
Val Val Ala Lys Val  Glu Lys Gly Lys Ser  Lys Lys Leu Lys Ser  Val 
                1365                 1370                 1375     
Lys Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser Ser Phe  Glu Lys 
            1380                 1385                 1390         
Asn Pro Ile  Asp Phe Leu Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys 
        1395                 1400                 1405             
Asp Leu  Ile Ile Lys Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn 
    1410                 1415                 1420                 
Gly  Arg Lys Arg Met Leu  Ala Ser Ala Lys Gln  Leu Gln Lys Gly Asn  
1425                 1430                 1435                 1440 
Glu Leu Ala Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser 
                1445                 1450                 1455     
His Tyr Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln 
            1460                 1465                 1470         
Leu Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
        1475                 1480                 1485             
Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Asp 
    1490                 1495                 1500                 
Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile Arg Glu  
1505                 1510                 1515                 1520 
Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Arg Leu Gly  Ala 
                1525                 1530                 1535     
Pro Arg Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp Pro Lys  Gln Tyr 
            1540                 1545                 1550         
Arg Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu Ile His  Gln Ser Ile 
        1555                 1560                 1565             
Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu Ser Gln  Leu Gly Gly Asp 
    1570                 1575                 1580                 
Ser  Gly Gly Ser Lys Arg  Thr Ala Asp Gly Ser  Glu Phe Glu Pro Lys  
1585                 1590                 1595                 1600 
Lys Lys Arg Lys Val  
                1605 

<210>  70
<211>  1605
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  8e-SpRY-HF融合蛋白的氨基酸序列


<400>  70
Met Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys 
1               5                   10                  15      
Arg Lys Val Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His 
            20                  25                  30          
Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val 
        35                  40                  45              
Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn 
    50                  55                  60                  
Arg Ala Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala 
65                  70                  75                  80  
Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala 
                85                  90                  95      
Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met 
            100                 105                 110         
Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ser Lys 
        115                 120                 125             
Arg Gly Ala Ala Gly Ser Leu Met Asn Val Leu Asn Tyr Pro Gly Met 
    130                 135                 140                 
Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala 
145                 150                 155                 160 
Ala Leu Leu Cys Asp Phe Tyr Arg Met Pro Arg Gln Val Phe Asn Ala 
                165                 170                 175     
Gln Lys Lys Ala Gln Ser Ser Ile Asn Ser Gly Gly Ser Ser Gly Gly 
            180                 185                 190         
Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
        195                 200                 205             
Ser Ser Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly 
    210                 215                 220                 
Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu 
225                 230                 235                 240 
Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg 
                245                 250                 255     
His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly 
            260                 265                 270         
Glu Thr Ala Glu Arg Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr 
        275                 280                 285             
Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn 
    290                 295                 300                 
Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser 
305                 310                 315                 320 
Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly 
                325                 330                 335     
Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr 
            340                 345                 350         
His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg 
        355                 360                 365             
Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe 
    370                 375                 380                 
Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu 
385                 390                 395                 400 
Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro 
                405                 410                 415     
Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu 
            420                 425                 430         
Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu 
        435                 440                 445             
Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu 
    450                 455                 460                 
Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu 
465                 470                 475                 480 
Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala 
                485                 490                 495     
Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu 
            500                 505                 510         
Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile 
        515                 520                 525             
Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His 
    530                 535                 540                 
His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro 
545                 550                 555                 560 
Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala 
                565                 570                 575     
Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile 
            580                 585                 590         
Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys 
        595                 600                 605             
Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly 
    610                 615                 620                 
Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg 
625                 630                 635                 640 
Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile 
                645                 650                 655     
Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala 
            660                 665                 670         
Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr 
        675                 680                 685             
Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala 
    690                 695                 700                 
Gln Ser Phe Ile Glu Arg Met Thr Ala Phe Asp Lys Asn Leu Pro Asn 
705                 710                 715                 720 
Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val 
                725                 730                 735     
Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys 
            740                 745                 750         
Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu 
        755                 760                 765             
Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr 
    770                 775                 780                 
Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu 
785                 790                 795                 800 
Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile 
                805                 810                 815     
Ile Lys Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu 
            820                 825                 830         
Glu Asp Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile 
        835                 840                 845             
Glu Glu Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met 
    850                 855                 860                 
Lys Gln Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Ala Leu Ser Arg 
865                 870                 875                 880 
Lys Leu Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu 
                885                 890                 895     
Asp Phe Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Ala Leu 
            900                 905                 910         
Ile His Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln 
        915                 920                 925             
Val Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala 
    930                 935                 940                 
Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val 
945                 950                 955                 960 
Asp Glu Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val 
                965                 970                 975     
Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn 
            980                 985                 990         
Ser Arg Glu Arg Met Lys Arg Ile  Glu Glu Gly Ile Lys  Glu Leu Gly 
        995                 1000                 1005             
Ser Gln  Ile Leu Lys Glu His  Pro Val Glu Asn Thr  Gln Leu Gln Asn 
    1010                 1015                 1020                 
Glu  Lys Leu Tyr Leu Tyr  Tyr Leu Gln Asn Gly  Arg Asp Met Tyr Val  
1025                 1030                 1035                 1040 
Asp Gln Glu Leu Asp  Ile Asn Arg Leu Ser  Asp Tyr Asp Val Asp  His 
                1045                 1050                 1055     
Ile Val Pro Gln  Ser Phe Leu Lys Asp  Asp Ser Ile Asp Asn  Lys Val 
            1060                 1065                 1070         
Leu Thr Arg  Ser Asp Lys Asn Arg  Gly Lys Ser Asp Asn  Val Pro Ser 
        1075                 1080                 1085             
Glu Glu  Val Val Lys Lys Met  Lys Asn Tyr Trp Arg  Gln Leu Leu Asn 
    1090                 1095                 1100                 
Ala  Lys Leu Ile Thr Gln  Arg Lys Phe Asp Asn  Leu Thr Lys Ala Glu  
1105                 1110                 1115                 1120 
Arg Gly Gly Leu Ser  Glu Leu Asp Lys Ala  Gly Phe Ile Lys Arg  Gln 
                1125                 1130                 1135     
Leu Val Glu Thr  Arg Ala Ile Thr Lys  His Val Ala Gln Ile  Leu Asp 
            1140                 1145                 1150         
Ser Arg Met  Asn Thr Lys Tyr Asp  Glu Asn Asp Lys Leu  Ile Arg Glu 
        1155                 1160                 1165             
Val Lys  Val Ile Thr Leu Lys  Ser Lys Leu Val Ser  Asp Phe Arg Lys 
    1170                 1175                 1180                 
Asp  Phe Gln Phe Tyr Lys  Val Arg Glu Ile Asn  Asn Tyr His His Ala  
1185                 1190                 1195                 1200 
His Asp Ala Tyr Leu  Asn Ala Val Val Gly  Thr Ala Leu Ile Lys  Lys 
                1205                 1210                 1215     
Tyr Pro Lys Leu  Glu Ser Glu Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr 
            1220                 1225                 1230         
Asp Val Arg  Lys Met Ile Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala 
        1235                 1240                 1245             
Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys Thr 
    1250                 1255                 1260                 
Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu Ile Glu  
1265                 1270                 1275                 1280 
Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly Arg Asp  Phe 
                1285                 1290                 1295     
Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val Asn Ile  Val Lys 
            1300                 1305                 1310         
Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys Glu Ser  Ile Arg Pro 
        1315                 1320                 1325             
Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg Lys Lys  Asp Trp Asp Pro 
    1330                 1335                 1340                 
Lys  Lys Tyr Gly Gly Phe  Leu Trp Pro Thr Val  Ala Tyr Ser Val Leu  
1345                 1350                 1355                 1360 
Val Val Ala Lys Val  Glu Lys Gly Lys Ser  Lys Lys Leu Lys Ser  Val 
                1365                 1370                 1375     
Lys Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser Ser Phe  Glu Lys 
            1380                 1385                 1390         
Asn Pro Ile  Asp Phe Leu Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys 
        1395                 1400                 1405             
Asp Leu  Ile Ile Lys Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn 
    1410                 1415                 1420                 
Gly  Arg Lys Arg Met Leu  Ala Ser Ala Lys Gln  Leu Gln Lys Gly Asn  
1425                 1430                 1435                 1440 
Glu Leu Ala Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser 
                1445                 1450                 1455     
His Tyr Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln 
            1460                 1465                 1470         
Leu Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
        1475                 1480                 1485             
Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Asp 
    1490                 1495                 1500                 
Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile Arg Glu  
1505                 1510                 1515                 1520 
Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Arg Leu Gly  Ala 
                1525                 1530                 1535     
Pro Arg Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp Pro Lys  Gln Tyr 
            1540                 1545                 1550         
Arg Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu Ile His  Gln Ser Ile 
        1555                 1560                 1565             
Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu Ser Gln  Leu Gly Gly Asp 
    1570                 1575                 1580                 
Ser  Gly Gly Ser Lys Arg  Thr Ala Asp Gly Ser  Glu Phe Glu Pro Lys  
1585                 1590                 1595                 1600 
Lys Lys Arg Lys Val  
                1605 

<210>  71
<211>  1605
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  V106W-SpRY-HF融合蛋白的氨基酸序列


<400>  71
Met Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys 
1               5                   10                  15      
Arg Lys Val Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His 
            20                  25                  30          
Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val 
        35                  40                  45              
Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn 
    50                  55                  60                  
Arg Ala Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala 
65                  70                  75                  80  
Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala 
                85                  90                  95      
Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met 
            100                 105                 110         
Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Trp Arg Asn Ser Lys 
        115                 120                 125             
Arg Gly Ala Ala Gly Ser Leu Met Asn Val Leu Asn Tyr Pro Gly Met 
    130                 135                 140                 
Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala 
145                 150                 155                 160 
Ala Leu Leu Cys Asp Phe Tyr Arg Met Pro Arg Gln Val Phe Asn Ala 
                165                 170                 175     
Gln Lys Lys Ala Gln Ser Ser Ile Asn Ser Gly Gly Ser Ser Gly Gly 
            180                 185                 190         
Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
        195                 200                 205             
Ser Ser Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly 
    210                 215                 220                 
Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu 
225                 230                 235                 240 
Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg 
                245                 250                 255     
His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly 
            260                 265                 270         
Glu Thr Ala Glu Arg Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr 
        275                 280                 285             
Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn 
    290                 295                 300                 
Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser 
305                 310                 315                 320 
Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly 
                325                 330                 335     
Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr 
            340                 345                 350         
His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg 
        355                 360                 365             
Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe 
    370                 375                 380                 
Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu 
385                 390                 395                 400 
Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro 
                405                 410                 415     
Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu 
            420                 425                 430         
Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu 
        435                 440                 445             
Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu 
    450                 455                 460                 
Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu 
465                 470                 475                 480 
Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala 
                485                 490                 495     
Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu 
            500                 505                 510         
Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile 
        515                 520                 525             
Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His 
    530                 535                 540                 
His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro 
545                 550                 555                 560 
Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala 
                565                 570                 575     
Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile 
            580                 585                 590         
Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys 
        595                 600                 605             
Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly 
    610                 615                 620                 
Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg 
625                 630                 635                 640 
Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile 
                645                 650                 655     
Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala 
            660                 665                 670         
Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr 
        675                 680                 685             
Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala 
    690                 695                 700                 
Gln Ser Phe Ile Glu Arg Met Thr Ala Phe Asp Lys Asn Leu Pro Asn 
705                 710                 715                 720 
Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val 
                725                 730                 735     
Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys 
            740                 745                 750         
Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu 
        755                 760                 765             
Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr 
    770                 775                 780                 
Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu 
785                 790                 795                 800 
Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile 
                805                 810                 815     
Ile Lys Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu 
            820                 825                 830         
Glu Asp Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile 
        835                 840                 845             
Glu Glu Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met 
    850                 855                 860                 
Lys Gln Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Ala Leu Ser Arg 
865                 870                 875                 880 
Lys Leu Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu 
                885                 890                 895     
Asp Phe Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Ala Leu 
            900                 905                 910         
Ile His Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln 
        915                 920                 925             
Val Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala 
    930                 935                 940                 
Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val 
945                 950                 955                 960 
Asp Glu Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val 
                965                 970                 975     
Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn 
            980                 985                 990         
Ser Arg Glu Arg Met Lys Arg Ile  Glu Glu Gly Ile Lys  Glu Leu Gly 
        995                 1000                 1005             
Ser Gln  Ile Leu Lys Glu His  Pro Val Glu Asn Thr  Gln Leu Gln Asn 
    1010                 1015                 1020                 
Glu  Lys Leu Tyr Leu Tyr  Tyr Leu Gln Asn Gly  Arg Asp Met Tyr Val  
1025                 1030                 1035                 1040 
Asp Gln Glu Leu Asp  Ile Asn Arg Leu Ser  Asp Tyr Asp Val Asp  His 
                1045                 1050                 1055     
Ile Val Pro Gln  Ser Phe Leu Lys Asp  Asp Ser Ile Asp Asn  Lys Val 
            1060                 1065                 1070         
Leu Thr Arg  Ser Asp Lys Asn Arg  Gly Lys Ser Asp Asn  Val Pro Ser 
        1075                 1080                 1085             
Glu Glu  Val Val Lys Lys Met  Lys Asn Tyr Trp Arg  Gln Leu Leu Asn 
    1090                 1095                 1100                 
Ala  Lys Leu Ile Thr Gln  Arg Lys Phe Asp Asn  Leu Thr Lys Ala Glu  
1105                 1110                 1115                 1120 
Arg Gly Gly Leu Ser  Glu Leu Asp Lys Ala  Gly Phe Ile Lys Arg  Gln 
                1125                 1130                 1135     
Leu Val Glu Thr  Arg Ala Ile Thr Lys  His Val Ala Gln Ile  Leu Asp 
            1140                 1145                 1150         
Ser Arg Met  Asn Thr Lys Tyr Asp  Glu Asn Asp Lys Leu  Ile Arg Glu 
        1155                 1160                 1165             
Val Lys  Val Ile Thr Leu Lys  Ser Lys Leu Val Ser  Asp Phe Arg Lys 
    1170                 1175                 1180                 
Asp  Phe Gln Phe Tyr Lys  Val Arg Glu Ile Asn  Asn Tyr His His Ala  
1185                 1190                 1195                 1200 
His Asp Ala Tyr Leu  Asn Ala Val Val Gly  Thr Ala Leu Ile Lys  Lys 
                1205                 1210                 1215     
Tyr Pro Lys Leu  Glu Ser Glu Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr 
            1220                 1225                 1230         
Asp Val Arg  Lys Met Ile Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala 
        1235                 1240                 1245             
Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys Thr 
    1250                 1255                 1260                 
Glu  Ile Thr Leu Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu Ile Glu  
1265                 1270                 1275                 1280 
Thr Asn Gly Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly Arg Asp  Phe 
                1285                 1290                 1295     
Ala Thr Val Arg  Lys Val Leu Ser Met  Pro Gln Val Asn Ile  Val Lys 
            1300                 1305                 1310         
Lys Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys Glu Ser  Ile Arg Pro 
        1315                 1320                 1325             
Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg Lys Lys  Asp Trp Asp Pro 
    1330                 1335                 1340                 
Lys  Lys Tyr Gly Gly Phe  Leu Trp Pro Thr Val  Ala Tyr Ser Val Leu  
1345                 1350                 1355                 1360 
Val Val Ala Lys Val  Glu Lys Gly Lys Ser  Lys Lys Leu Lys Ser  Val 
                1365                 1370                 1375     
Lys Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser Ser Phe  Glu Lys 
            1380                 1385                 1390         
Asn Pro Ile  Asp Phe Leu Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys 
        1395                 1400                 1405             
Asp Leu  Ile Ile Lys Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu Asn 
    1410                 1415                 1420                 
Gly  Arg Lys Arg Met Leu  Ala Ser Ala Lys Gln  Leu Gln Lys Gly Asn  
1425                 1430                 1435                 1440 
Glu Leu Ala Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr Leu Ala  Ser 
                1445                 1450                 1455     
His Tyr Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn Glu Gln  Lys Gln 
            1460                 1465                 1470         
Leu Phe Val  Glu Gln His Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln 
        1475                 1480                 1485             
Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Asp 
    1490                 1495                 1500                 
Lys  Val Leu Ser Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile Arg Glu  
1505                 1510                 1515                 1520 
Gln Ala Glu Asn Ile  Ile His Leu Phe Thr  Leu Thr Arg Leu Gly  Ala 
                1525                 1530                 1535     
Pro Arg Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp Pro Lys  Gln Tyr 
            1540                 1545                 1550         
Arg Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu Ile His  Gln Ser Ile 
        1555                 1560                 1565             
Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu Ser Gln  Leu Gly Gly Asp 
    1570                 1575                 1580                 
Ser  Gly Gly Ser Lys Arg  Thr Ala Asp Gly Ser  Glu Phe Glu Pro Lys  
1585                 1590                 1595                 1600 
Lys Lys Arg Lys Val  
                1605 

<210>  72
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg1的鉴定上游引物


<400>  72
gagtagccaa agaccatcag                                                    20

<210>  73
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg1的鉴定下游引物


<400>  73
cttccataac aaaaggaagc                                                    20

<210>  74
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg2的鉴定上游引物


<400>  74
tgtttttgtg gaacatggtg                                                    20

<210>  76
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg2的鉴定下游引物


<400>  76
gaatcaggca agaaataagc                                                    20

<210>  76
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg3的鉴定上游引物


<400>  76
gacttgtgga gatgcagcga                                                    20

<210>  103
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg3的鉴定下游引物


<400>  103
cactcaccat gttccacaaa                                                    20

<210>  77
<211>  19
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg4的鉴定上游引物	


<400>  77
acagggtttt gccgtgttg                                                     19

<210>  78
<211>  18
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg4的鉴定下游引物


<400>  78
gcccgttagg actgcttg                                                      18

<210>  79
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg5的鉴定上游引物


<400>  79
cttgtggaga tgcagcgaat                                                    20

<210>  80
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg5的鉴定下游引物


<400>  80
aagcactcac catgttccac                                                    20

<210>  81
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg6的鉴定上游引物


<400>  81
gtggaacatg gtgagtgctt                                                    20

<210>  82
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg6的鉴定下游引物


<400>  82
tagcagagga atcaggcaag                                                    20

<210>  83
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg7的鉴定上游引物


<400>  83
acactgcaat gtttttgtgg                                                    20

<210>  84
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg7的鉴定下游引物


<400>  84
aggcaagaaa taagcaactc                                                    20

<210>  85
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg8的鉴定上游引物


<400>  85
gtggagatgc agcgaatgtg                                                    20

<210>  86
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg8的鉴定下游引物


<400>  86
gaaaagcact caccatgttc                                                    20

<210>  87
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg9的鉴定上游引物


<400>  87
gcttcctttt gttatggaag                                                    20

<210>  88
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg9的鉴定下游引物


<400>  88
gatgattcct tcttccctgc                                                    20

<210>  89
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg10的鉴定上游引物


<400>  89
atgggggtcc acactgcaat                                                    20

<210>  90
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg10的鉴定下游引物


<400>  90
ccgaagtcac gaagtaactt                                                    20

<210>  91
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg11的鉴定上游引物


<400>  91
gtgcttcctt ttgttatgga                                                    20

<210>  92
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg11的鉴定下游引物


<400>  92
gattccttct tccctgctcc                                                    20

<210>  93
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg12的鉴定上游引物


<400>  93
ctggaaaggg gtacctatta                                                    20

<210>  94
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg12的鉴定下游引物


<400>  94
ttggaaggaa ctgtcgcaag                                                    20

<210>  95
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg13的鉴定上游引物


<400>  95
agatcttgaa ccctcttctg                                                    20

<210>  96
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg13的鉴定下游引物


<400>  96
gttcaaagtt ccttgaaggc                                                    20

<210>  97
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg14的鉴定上游引物


<400>  97
gaggagtagc caaagaccat                                                    20

<210>  98
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg14的鉴定下游引物


<400>  98
tccataacaa aaggaagcac                                                    20

<210>  99
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg15的鉴定上游引物


<400>  99
tgaaccctct tctggaaagg                                                    20

<210>  100
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg15的鉴定下游引物


<400>  100
ctgttgttca aagttccttg                                                    20

<210>  101
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg16的鉴定上游引物


<400>  101
ggcgtggcag ctgatatccg                                                    20

<210>  102
<211>  23
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg16的鉴定下游引物


<400>  102
gtgtttcaca aaacaggggt ggc                                                23

<210>  104
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg17的鉴定上游引物


<400>  104
gagcatctga gtccagggga                                                    20

<210>  105
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg17的鉴定下游引物


<400>  105
gacagggtag acggcagttc                                                    20

<210>  106
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg18的鉴定上游引物


<400>  106
cactccttgt gccccattgg                                                    20

<210>  107
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg18的鉴定下游引物


<400>  107
gttgtagtag tcgcgactct                                                    20

<210>  108
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg19的鉴定上游引物


<400>  108
cagtatccga aggcagcagc                                                    20

<210>  109
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg19的鉴定下游引物


<400>  109
cattcggcca atggggcaca                                                    20

<210>  110
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg20的鉴定上游引物


<400>  110
tacttagggg gcacttcgac                                                    20

<210>  111
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg20的鉴定下游引物


<400>  111
aaggggaata ctcagcagta                                                    20

<210>  112
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg21的鉴定上游引物


<400>  112
cgttggagca tctgagtcca                                                    20

<210>  113
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg21的鉴定下游引物


<400>  113
gacggcagtt caagtgtccc                                                    20

<210>  114
<211>  18
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg22的鉴定下游引物


<400>  114
tgttcacggg ctgcgctg                                                      18

<210>  115
<211>  19
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg22的鉴定下游引物


<400>  115
ggtggatgca gctttgcgg                                                     19

<210>  116
<211>  23
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg23的鉴定上游引物


<400>  116
ttaatgagtg gcatcttggc ata                                                23

<210>  117
<211>  24
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg23的鉴定下游引物


<400>  117
ttgcaccaaa atggttagta aaac                                               24

<210>  118
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg24的鉴定上游引物	


<400>  118
tggagcatct gagtccaggg                                                    20

<210>  119
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg24的鉴定下游引物


<400>  119
ggtagacggc agttcaagtg                                                    20

<210>  120
<211>  23
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg25的鉴定上游引物


<400>  120
ctttgagatg gaatggagtt ttg                                                23

<210>  121
<211>  22
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg25的鉴定下游引物


<400>  121
agaaacatga actgtgagat gc                                                 22

<210>  122
<211>  18
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg26的鉴定上游引物


<400>  122
gacacaagca gggacacg                                                      18

<210>  123
<211>  21
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg26的鉴定下游引物


<400>  123
ctactctgga ggcgaaaaag g                                                  21

<210>  124
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg27的鉴定上游引物


<400>  124
cgccaaggag ttgtgtaagg                                                    20

<210>  125
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg27的鉴定下游引物


<400>  125
tctcgccttc tagccctttg                                                    20

<210>  126
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg28的鉴定上游引物


<400>  126
gaacagcttc ggggggattg                                                    20

<210>  127
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg28的鉴定下游引物


<400>  127
cttgtagaga gacagggtag                                                    20

<210>  128
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg29的鉴定上游引物


<400>  128
gggattgcat gtacgcccca                                                    20

<210>  129
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg29的鉴定下游引物


<400>  129
ccagtgctcc ggacttgtag                                                    20

<210>  130
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg30的鉴定上游引物


<400>  130
ggcacttcga ccatttctga                                                    20

<210>  131
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg30的鉴定下游引物


<400>  131
tatcttcagt gctcttgcct                                                    20

<210>  132
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg31的鉴定上游引物


<400>  132
ccgaatgcaa aggttctctg                                                    20

<210>  133
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg31的鉴定下游引物


<400>  133
ccagagccag tggaaagttg                                                    20

<210>  134
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg32的鉴定上游引物


<400>  134
gtacgcccca cttttgggag                                                    20

<210>  135
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg32的鉴定下游引物


<400>  135
gtccagtgct ccggacttgt                                                    20

<210>  136
<211>  21
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg32的鉴定上游引物


<400>  136
cttcacaggc taccccctaa g                                                  21

<210>  137
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg32的鉴定下游引物


<400>  137
gaaaattacc acatgctgtc                                                    20

<210>  138
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg34的鉴定上游引物


<400>  138
atgtggtaat tttccagccc                                                    20

<210>  139
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg34的鉴定下游引物


<400>  139
caagcacatg acagttaagg                                                    20

<210>  140
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg35的鉴定上游引物


<400>  140
gatcttcaca ggctaccccc                                                    20

<210>  141
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg35的鉴定下游引物


<400>  141
ccacatgctg tcacagttag                                                    20

<210>  142
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg36的鉴定上游引物


<400>  142
gcagtgttta gaaggagact                                                    20

<210>  143
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg36的鉴定下游引物


<400>  143
gttggtagaa tggcagtgca                                                    20

<210>  144
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg37的鉴定上游引物


<400>  144
cacaggctac cccctaagtc                                                    20

<210>  145
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg37的鉴定下游引物


<400>  145
ggctggaaaa ttaccacatg                                                    20

<210>  146
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg38的鉴定上游引物


<400>  146
cagcatgtgg taattttcca                                                    20

<210>  147
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg38的鉴定下游引物


<400>  147
cacatgacag ttaaggtttg                                                    20

<210>  148
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg39的鉴定上游引物


<400>  148
caagacctgg ctgagctaac                                                    20

<210>  149
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg39的鉴定下游引物


<400>  149
tttagtcttt caagcaggtg                                                    20

<210>  150
<211>  22
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg40的鉴定上游引物


<400>  150
aattgtttga acctgggagt tg                                                 22

<210>  151
<211>  24
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg40的鉴定下游引物


<400>  151
gttgatagcc tcacagaaag taac                                               24

<210>  152
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg41的鉴定上游引物


<400>  152
cctaaaccag tgtcagggag                                                    20

<210>  153
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg41的鉴定下游引物


<400>  153
tgctttgtgt tccagtttcc                                                    20

<210>  154
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg42的鉴定上游引物


<400>  154
agaaggagac ttgtgcacat                                                    20

<210>  155
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg42的鉴定下游引物


<400>  155
attgttggta gaatggcagt                                                    20

<210>  156
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg43的鉴定上游引物


<400>  156
gtttagaagg agacttgtgc                                                    20

<210>  157
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg43的鉴定下游引物


<400>  157
ggtagaatgg cagtgcaata                                                    20

<210>  158
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg44的鉴定上游引物


<400>  158
aaagatcttc acaggctacc                                                    20

<210>  159
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg44的鉴定下游引物


<400>  159
ccagcgggct ggaaaattac                                                    20

<210>  160
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg45的鉴定上游引物


<400>  160
ggagacttgt gcacattcta                                                    20

<210>  161
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg45的鉴定下游引物


<400>  161
tctattgttg gtagaatggc                                                    20

<210>  162
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg46的鉴定上游引物


<400>  162
ggccctgtaa aggaaactgg                                                    20

<210>  163
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg46的鉴定下游引物


<400>  163
tgtttagtct ttcaagcagg                                                    20

<210>  164
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg47的鉴定上游引物


<400>  164
atgataacaa gacctggctg                                                    20

<210>  165
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg47的鉴定下游引物


<400>  165
tctttcaagc aggtgattac                                                    20

<210>  166
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg48的鉴定上游引物


<400>  166
ataacaagac ctggctgagc                                                    20

<210>  167
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  sg48的鉴定下游引物


<400>  167
tcaagcaggt gattacagga                                                    20

<210>  168
<211>  22
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  PAH Mut-sgRNA


<400>  168
cctccaacct gtggctggcc tg                                                 22

<210>  169
<211>  22
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  PAH Rec-sgRNA1


<400>  169
tccgcctcca acctgtggct gg                                                 22

<210>  170
<211>  22
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  PAH Rec-sgRNA2


<400>  170
ccgcctccaa cctgtggctg gc                                                 22

<210>  171
<211>  22
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  PAH Rec-sgRNA3


<400>  171
cgcctccaac ctgtggctgg cc                                                 22

<210>  172
<211>  22
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  PAH Rec-sgRNA4


<400>  172
gcctccaacc tgtggctggc ct                                                 22

<210>  173
<211>  22
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  PAH Rec-sgRNA5


<400>  173
cctccaacct gtggctggcc tg                                                 22

<210>  174
<211>  22
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  PAH Rec-sgRNA6


<400>  174
ctccaacctg tggctggcct gc                                                 22

<210>  175
<211>  22
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  PAH Rec-sgRNA7	


<400>  175
tccaacctgt ggctggcctg ct                                                 22

<210>  176
<211>  22
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  PAH Rec-sgRNA8


<400>  176
ccaacctgtg gctggcctgc tt                                                 22

<210>  177
<211>  22
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  PAH 扩增上游引物


<400>  177
gtccctgggc agttatgtgt ac                                                 22

<210>  178
<211>  21
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  PAH 扩增下游引物


<400>  178
caactggtag ctggaggaca g                                                  21

