                         SEQUENCE LISTING

<110>  The Government of the United States of America, as 
       Represented by the Secretary of the Navy
 
<120>  Single Domain Antibodies that Bind and Neutralize Venezuelan 
       Equine Encephalitis Virus

<130>  Navy Case 113775

<150>  US 63/224,642
<151>  2021-07-22

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  1

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Val Ser Ile Asn 
            20                  25                  30          


Val Met Ala Trp Tyr Arg Gln Ser Pro Gly Lys Glu Arg Glu Leu Val 
        35                  40                  45              


Ala Lys Ser Ser Gly Pro Phe Thr Leu Tyr Ala Asp Ser Val Lys Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Asn Asp Ala Ala Lys Asn Thr Val Asp Leu Gln 
65                  70                  75                  80  


Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn Ala 
                85                  90                  95      


Glu Gly Leu Arg Tyr Pro Ser Gly Thr Tyr Gly Pro Ser Ala Val Trp 
            100                 105                 110         


Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120         


<210>  2
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  2

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Lys Val Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Ser Leu 
            20                  25                  30          


Ala Met Ala Trp Phe Arg Glu Ala Pro Gly Asn Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Ala Ile Met Trp Thr Gly Asp Arg Thr His Tyr Ala Asp Phe Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Leu Asn Thr Val Ser 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Lys Pro Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Gly Ala Phe Ser Phe Pro Ser Gln Phe Ala Arg Asp Tyr Thr Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Gln Val Ser Val Ser Ser 
        115                 120             


<210>  3
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  3

Asp Val Gln Leu Gln Ala Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gln Asn Leu Phe Glu Tyr Tyr 
            20                  25                  30          


Thr Met Gly Trp Tyr Arg Gln Val Pro Gly Ser Gln Arg Glu Arg Val 
        35                  40                  45              


Ala Leu Ile Asn Asn Gly Gly Ser Asn Val Ala Gly Ser Val Glu Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Lys Asp Asn Ala Lys Asn Ser Ile Tyr Leu Gln 
65                  70                  75                  80  


Met Asn Asn Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr Cys Arg Ala 
                85                  90                  95      


Phe Gly Pro Ala Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
            100                 105                 110         


Ser 
    


<210>  4
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  4

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Lys Val Ser Cys Ala Ala Ser Gly Arg Thr Phe Asn Asn Leu 
            20                  25                  30          


Ala Met Ala Trp Phe Arg Glu Ala Pro Glu Asn Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Ala Ile Met Trp Thr Gly Asp Arg Thr His Tyr Ala Asp Phe Val 
    50                  55                  60                  


Lys Gly Arg Tyr Thr Ile Ser Arg Asp Asn Ala Leu Asn Thr Val Ser 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Lys Pro Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Gly Ala Phe Ser Phe Pro Ser Gln Phe Ala Arg Asp Tyr Thr Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Gln Val Ser Val Ser Ser 
        115                 120             


<210>  5
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  5

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Val Ser Ile Asn 
            20                  25                  30          


Val Met Ala Trp Tyr Arg Gln Ser Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Lys Ser Ser Gly Pro Phe Thr Leu Tyr Ala Asp Ser Val Lys Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Asn Asp Ala Ala Lys Asn Thr Val Asp Leu Gln 
65                  70                  75                  80  


Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn Ala 
                85                  90                  95      


Glu Gly Leu Arg Tyr Pro Ser Gly Thr Tyr Gly Pro Ser Ala Val Trp 
            100                 105                 110         


Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120         


<210>  6
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  6

Asp Val Gln Leu Gln Ala Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gln Asn Leu Phe Glu Tyr Tyr 
            20                  25                  30          


Thr Met Gly Trp Tyr Arg Gln Val Pro Gly Ser Gln Arg Glu Arg Val 
        35                  40                  45              


Ala Leu Ile Asn Asn Gly Gly Ser Asn Val Ala Gly Ser Val Glu Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Ile Tyr Leu Gln 
65                  70                  75                  80  


Met Asn Asn Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr Cys Arg Ala 
                85                  90                  95      


Phe Gly Pro Ala Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
            100                 105                 110         


Ser 
    


<210>  7
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  7

Asp Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ile Lys Gly Tyr 
            20                  25                  30          


Ala Val Gly Trp Phe Arg Gln Ala Ser Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Val Ile Ser Tyr Phe Asp Glu Arg Ala Asp Tyr Ala His Ser Ala 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asp Thr Val Val 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Ala Gly Leu Ser Glu Ser Thr Leu Pro Ser Glu Tyr Ile Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Gln Val Ser Val Ser Ser 
        115                 120         


<210>  8
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  8

Asp Val Gln Leu Gln Ala Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly His Ser Phe Ala Asn Tyr 
            20                  25                  30          


His Val Ala Trp Ile Arg Gln Thr Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Gly Ser Ala Ser Arg Arg Asp Asp Ser Thr Tyr Tyr Ala Asp Phe Ala 
    50                  55                  60                  


Leu Gly Arg Phe Ile Ile Ser Arg Asp Asn Asp Ile Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Val Ala Ala Val Met Ala Gln Thr Thr Gln Gly Trp Thr Thr Asp Tyr 
            100                 105                 110         


Asp Leu Arg Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125 


<210>  9
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  9

Asp Val Gln Leu Gln Ala Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly His Ser Phe Ala Asn Tyr 
            20                  25                  30          


His Val Ala Trp Ile Arg Gln Thr Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Gly Ser Ala Ser Arg Arg Asp Asp Ser Thr Tyr Tyr Ala Asp Phe Ala 
    50                  55                  60                  


Leu Gly Arg Phe Ile Ile Ser Arg Asp Asn Asp Ile Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Val Ala Ala Val Met Ala Gln Thr Thr Gln Gly Trp Thr Thr Asp Tyr 
            100                 105                 110         


Asp Leu Arg Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125 


<210>  10
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  10

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Val Ser Ile Asn 
            20                  25                  30          


Val Met Ala Trp Tyr Arg Gln Ser Pro Gly Lys Glu Arg Glu Leu Val 
        35                  40                  45              


Ala Lys Ser Ser Gly Pro Phe Thr Leu Tyr Ala Asp Ser Val Lys Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Asn Asp Ala Ala Lys Asn Thr Val Asp Leu Gln 
65                  70                  75                  80  


Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn Ala 
                85                  90                  95      


Glu Gly Leu Arg Tyr Pro Ser Gly Thr Tyr Gly Pro Ser Ala Val Trp 
            100                 105                 110         


Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala Ala Ala Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ser Asp Val 
    130                 135                 140                 


Gln Leu Gln Ala Ser Gly Gly Gly Ser Val Gln Ala Gly Gly Ser Leu 
145                 150                 155                 160 


Arg Leu Ser Cys Ala Ala Ser Gly His Ser Phe Ala Asn Tyr His Val 
                165                 170                 175     


Ala Trp Ile Arg Gln Thr Pro Gly Lys Glu Arg Glu Phe Val Gly Ser 
            180                 185                 190         


Ala Ser Arg Arg Asp Asp Ser Thr Tyr Tyr Ala Asp Phe Ala Leu Gly 
        195                 200                 205             


Arg Phe Ile Ile Ser Arg Asp Asn Asp Ile Asn Thr Ala Tyr Leu Gln 
    210                 215                 220                 


Met Asn Ser Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr Cys Val Ala 
225                 230                 235                 240 


Ala Val Met Ala Gln Thr Thr Gln Gly Trp Thr Thr Asp Tyr Asp Leu 
                245                 250                 255     


Arg Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
            260                 265         


<210>  11
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  11

Asp Val Gln Leu Gln Ala Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly His Ser Phe Ala Asn Tyr 
            20                  25                  30          


His Val Ala Trp Ile Arg Gln Thr Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Gly Ser Ala Ser Arg Arg Asp Asp Ser Thr Tyr Tyr Ala Asp Phe Ala 
    50                  55                  60                  


Leu Gly Arg Phe Ile Ile Ser Arg Asp Asn Asp Ile Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Val Ala Ala Val Met Ala Gln Thr Thr Gln Gly Trp Thr Thr Asp Tyr 
            100                 105                 110         


Asp Leu Arg Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala Ala Ala 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Ser Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly 
145                 150                 155                 160 


Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Val Ser Ile 
                165                 170                 175     


Asn Val Met Ala Trp Tyr Arg Gln Ser Pro Gly Lys Glu Arg Glu Leu 
            180                 185                 190         


Val Ala Lys Ser Ser Gly Pro Phe Thr Leu Tyr Ala Asp Ser Val Lys 
        195                 200                 205             


Gly Arg Phe Thr Ile Ser Asn Asp Ala Ala Lys Asn Thr Val Asp Leu 
    210                 215                 220                 


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
225                 230                 235                 240 


Ala Glu Gly Leu Arg Tyr Pro Ser Gly Thr Tyr Gly Pro Ser Ala Val 
                245                 250                 255     


Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
            260                 265         


<210>  12
<211>  131
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (25)..(26)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (32)..(41)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (45)..(47)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (49)..(51)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (53)..(53)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (55)..(67)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (69)..(72)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (76)..(76)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (79)..(79)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (81)..(87)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (92)..(92)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (96)..(96)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (98)..(98)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (102)..(102)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (104)..(121)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (128)..(128)
<223>  Xaa can be any naturally occurring amino acid

<400>  12

Xaa Val Gln Leu Gln Ala Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Xaa Val Gln Ala Gly Xaa Ser Leu Xaa Xaa Ser Cys Xaa Ala Ser Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Trp Xaa Arg Xaa Xaa Xaa Gly 
        35                  40                  45              


Xaa Xaa Xaa Glu Xaa Val Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    50                  55                  60                  


Xaa Xaa Xaa Ala Xaa Xaa Xaa Xaa Gly Arg Phe Xaa Ile Ser Xaa Asp 
65                  70                  75                  80  


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Leu Gln Met Asn Xaa Leu Lys Pro Xaa 
                85                  90                  95      


Asp Xaa Ala Val Tyr Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            100                 105                 110         


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Gly Gln Gly Thr Gln Val Xaa 
        115                 120                 125             


Val Ser Ser 
    130     


<210>  13
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  13

Ala Ala Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
1               5                   10                  15      


Gly Ser Gly Ser 
            20  


<210>  14
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  14

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Thr Ser Gln Asn Leu Phe Glu Tyr Tyr 
            20                  25                  30          


Thr Met Gly Trp Tyr Arg Gln Val Pro Gly Ser Gln Arg Glu Arg Val 
        35                  40                  45              


Ala Leu Ile Asn Asn Gly Gly Ser Thr Val Ala Gly Ser Val Glu Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Arg Asp His Ala Lys Asn Ser Val Tyr Leu Gln 
65                  70                  75                  80  


Met Asn Tyr Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr Cys Arg Ala 
                85                  90                  95      


Phe Gly Pro Ala Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
            100                 105                 110         


Ser 
    


