                         б

<110>  ()Ƽ޹˾
 
<120>  SurvivinArmored CAR-Tϸ俹Ӧ

<130>  PF 200352PCT

<160>  25    

<170>  PatentIn version 3.3

<210>  1
<211>  1467
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic

<400>  1
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg     60

atcccagaca tccagatgac acagactaca tcctccctgt ctgcctctct gggagacaga    120

gtcaccatca gttgcagggc aagtcaggac attagtaaat atttaaattg gtatcagcag    180

aaaccagatg gaactgttaa actcctgatc taccatacat caagattaca ctcaggagtc    240

ccatcaaggt tcagtggcag tgggtctgga acagattatt ctctcaccat tagcaacctg    300

gagcaagaag atattgccac ttacttttgc caacagggta atacgcttcc gtacacgttc    360

ggagggggga ctaagttgga aataacaggc tccacctctg gatccggcaa gcccggatct    420

ggcgagggat ccaccaaggg cgaggtgaaa ctgcaggagt caggacctgg cctggtggcg    480

ccctcacaga gcctgtccgt cacatgcact gtctcagggg tctcattacc cgactatggt    540

gtaagctgga ttcgccagcc tccacgaaag ggtctggagt ggctgggagt aatatggggt    600

agtgaaacca catactataa ttcagctctc aaatccagac tgaccatcat caaggacaac    660

tccaagagcc aagttttctt aaaaatgaac agtctgcaaa ctgatgacac agccatttac    720

tactgtgcca aacattatta ctacggtggt agctatgcta tggactactg gggtcaagga    780

acctcagtca ccgtctcctc agcggccgca attgaagtta tgtatcctcc tccttaccta    840

gacaatgaga agagcaatgg aaccattatc catgtgaaag ggaaacacct ttgtccaagt    900

cccctatttc ccggaccttc taagcccttt tgggtgctgg tggtggttgg gggagtcctg    960

gcttgctata gcttgctagt aacagtggcc tttattattt tctgggtgag gagtaagagg   1020

agcaggctcc tgcacagtga ctacatgaac atgactcccc gccgccccgg gcccacccgc   1080

aagcattacc agccctatgc cccaccacgc gacttcgcag cctatcgctc cagagtgaag   1140

ttcagcagga gcgcagacgc ccccgcgtac cagcagggcc agaaccagct ctataacgag   1200

ctcaatctag gacgaagaga ggagtacgat gttttggaca agagacgtgg ccgggaccct   1260

gagatggggg gaaagccgag aaggaagaac cctcaggaag gcctgtacaa tgaactgcag   1320

aaagataaga tggcggaggc ctacagtgag attgggatga aaggcgagcg ccggaggggc   1380

aaggggcacg atggccttta ccagggtctc agtacagcca ccaaggacac ctacgacgcc   1440

cttcacatgc aggccctgcc ccctcgc                                       1467


<210>  2
<211>  429
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic

<400>  2
atgggtgccc cgacgttgcc ccctgcctgg cagccctttc tcaaggacca ccgcatctct     60

acattcaaga actggccctt cttggagggc tgcgcctgca ccccggagcg gatggccgag    120

gctggcttca tccactgccc cactgagaac gagccagact tggcccagtg tttcttctgc    180

ttcaaggagc tggaaggctg ggagccagat gacgacccca tagaggaaca taaaaagcat    240

tcgtccggtt gcgctttcct ttctgtcaag aagcagtttg aagaattaac ccttggtgaa    300

tttttgaaac tggacagaga aagagccaag aacaaaattg caaaggaaac caacaataag    360

aagaaagaat ttgaggaaac tgcggagaaa gtgcgccgtg ccatcgagca gctggctgcc    420

atggattga                                                            429


<210>  3
<211>  450
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic

<400>  3
atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt     60

tcgcccagcc aggaaatcca tgcccgattc agaagaggag ccagagcgaa ctgggtgaac    120

gtgatctcgg acctgaagaa gatcgaggac ctcatccagt cgatgcacat cgacgcgacg    180

ctgtacacgg agtcggacgt ccacccgtcg tgcaaggtca cggcgatgaa gtgcttcctc    240

ctggagctcc aagtcatctc gctcgagtcg ggggacgcgt cgatccacga cacggtggag    300

aacctgatca tcctggcgaa caactcgctg tcgtcgaacg ggaacgtcac ggagtcgggc    360

tgcaaggagt gcgaggagct ggaggagaag aacatcaagg agttcctgca gtcgttcgtg    420

cacatcgtcc agatgttcat gaacacgtcg                                     450


<210>  4
<211>  489
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  4

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
            100                 105                 110         


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser 
    130                 135                 140                 


Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala 
145                 150                 155                 160 


Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu 
                165                 170                 175     


Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu 
            180                 185                 190         


Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser 
        195                 200                 205             


Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln 
    210                 215                 220                 


Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 
225                 230                 235                 240 


Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Ala Ala Ile Glu 
            260                 265                 270         


Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr 
        275                 280                 285             


Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro 
    290                 295                 300                 


Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
305                 310                 315                 320 


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
                325                 330                 335     


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
            340                 345                 350         


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
        355                 360                 365             


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
    370                 375                 380                 


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
385                 390                 395                 400 


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
                405                 410                 415     


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
            420                 425                 430         


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
        435                 440                 445             


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
    450                 455                 460                 


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
465                 470                 475                 480 


Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 


<210>  5
<211>  142
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  5

Met Gly Ala Pro Thr Leu Pro Pro Ala Trp Gln Pro Phe Leu Lys Asp 
1               5                   10                  15      


His Arg Ile Ser Thr Phe Lys Asn Trp Pro Phe Leu Glu Gly Cys Ala 
            20                  25                  30          


Cys Thr Pro Glu Arg Met Ala Glu Ala Gly Phe Ile His Cys Pro Thr 
        35                  40                  45              


Glu Asn Glu Pro Asp Leu Ala Gln Cys Phe Phe Cys Phe Lys Glu Leu 
    50                  55                  60                  


Glu Gly Trp Glu Pro Asp Asp Asp Pro Ile Glu Glu His Lys Lys His 
65                  70                  75                  80  


Ser Ser Gly Cys Ala Phe Leu Ser Val Lys Lys Gln Phe Glu Glu Leu 
                85                  90                  95      


Thr Leu Gly Glu Phe Leu Lys Leu Asp Arg Glu Arg Ala Lys Asn Lys 
            100                 105                 110         


Ile Ala Lys Glu Thr Asn Asn Lys Lys Lys Glu Phe Glu Glu Thr Ala 
        115                 120                 125             


Glu Lys Val Arg Arg Ala Ile Glu Gln Leu Ala Ala Met Asp 
    130                 135                 140         


<210>  6
<211>  150
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  6

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Phe Arg Arg 
            20                  25                  30          


Gly Ala Arg Ala Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile 
        35                  40                  45              


Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu 
    50                  55                  60                  


Ser Asp Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu 
65                  70                  75                  80  


Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His 
                85                  90                  95      


Asp Thr Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser 
            100                 105                 110         


Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu 
        115                 120                 125             


Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln 
    130                 135                 140                 


Met Phe Met Asn Thr Ser 
145                 150 


<210>  7
<211>  22
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  7

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro 
            20          


<210>  8
<211>  107
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  8

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
            100                 105         


<210>  9
<211>  120
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  9

Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
            20                  25                  30          


Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  


Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  


Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala 
                85                  90                  95      


Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser 
        115                 120 


<210>  10
<211>  18
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  10

Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      


Lys Gly 
        


<210>  11
<211>  245
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  11

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Ser Thr Ser Gly 
            100                 105                 110         


Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Glu Val Lys 
        115                 120                 125             


Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser 
    130                 135                 140                 


Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser 
145                 150                 155                 160 


Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile 
                165                 170                 175     


Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu 
            180                 185                 190         


Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn 
        195                 200                 205             


Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr 
    210                 215                 220                 


Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser 
225                 230                 235                 240 


Val Thr Val Ser Ser 
                245 


<210>  12
<211>  39
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  12

Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
1               5                   10                  15      


Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
            20                  25                  30          


Phe Pro Gly Pro Ser Lys Pro 
        35                  


<210>  13
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  13

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  14
<211>  41
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  14

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  15
<211>  112
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  15

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  16
<211>  19
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  16

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  17
<211>  18
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  17

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  18
<211>  20
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  18

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  19
<211>  22
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  19

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  20
<211>  41
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic

<400>  20
ccatcctcta gactgccatg ggtgccccga cgttgccccc t                         41


<210>  21
<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic

<400>  21
caaattggac taatccggat cctcaatcca tggcagccag ctgct                     45


<210>  22
<211>  33
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic

<400>  22
aatcctggac cgatggatgc aatgaagaga ggg                                  33


<210>  23
<211>  44
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic

<400>  23
aatccggatc ctcacgacgt gttcatgaac atctggacga tgtg                      44


<210>  24
<211>  39
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic

<400>  24
ccatcctcta gactgccatg gctctcccag tgactgccc                            39


<210>  25
<211>  68
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic

<400>  25
gcttgtttca gcagagagaa gtttgttgcg ccggatccct cgaggcgagg gggcagggcc     60

tgcatgtg                                                              68


