                         SEQUENCE LISTING

<110>  BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
       ELLINGTON, Andrew
       D'OELSNITZ, Simon
       KAR, Shaunak
       THYER, Ross
 
<120>  METHODS AND COMPOSITIONS RELATED TO ENGINEERED BIOSENSORS

<130>  10046-413WO1

<140>  PCT/US2022/031957
<141>  2022-02-06

<150>  63/196,001
<151>  2021-06-21

<160>  18    

<170>  AlignSequences Software Suite VER 2.313

<210>  1
<211>  194
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Description of sequence: peptide sensor

<220>
<223>   Name: GLAU4

<220>
<223>   This sensor binds to glaucine

<400>  1

Met Val Ala Arg Pro Lys Ser Glu Asp Lys Lys Gln Ala Leu Leu Glu 
1               5                   10                  15      


Ala Ala Thr Gln Ala Ile Ala Gln Ser Gly Ile Ala Ala Ser Thr Ala 
            20                  25                  30          


Val Ile Ala Arg Asn Ala Gly Val Ala Glu Gly Thr Leu Phe Arg Tyr 
        35                  40                  45              


Phe Ala Thr Lys Asp Glu Leu Ile Asn Thr Leu Tyr Leu His Leu Thr 
    50                  55                  60                  


Gln Asp Trp Cys Gln Ser Ile Ile Met Glu Leu Asp Arg Ser Ile Thr 
65                  70                  75                  80  


Asp Ala Lys Met Met Thr Arg Phe Leu Trp Asn Ser Trp Ile Ser Trp 
                85                  90                  95      


Gly Leu Asn His Pro Ala Arg His Arg Ala Ile Arg Gln Leu Ala Val 
            100                 105                 110         


Ser Glu Lys Leu Thr Lys Glu Thr Glu Gln Arg Ala Asp Asp Met Phe 
        115                 120                 125             


Pro Glu Leu Arg Asp His Leu His Arg Asn Val Leu Met Val Phe Met 
    130                 135                 140                 


Ser Asp Glu Tyr Arg Ala Phe Gly Asp Gly Leu Phe Leu Ala Leu Ala 
145                 150                 155                 160 


Glu Thr Thr Met Asp Phe Ala Ala Arg Asp Pro Ala Arg Ala Gly Glu 
                165                 170                 175     


Tyr Ile Ala Leu Gly Phe Glu Ala Met Trp Arg Ala Leu Ala Arg Glu 
            180                 185                 190         


Glu Gln 
        


<210>  2
<211>  194
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Description of sequence: peptide sensor

<220>
<223>   Name: NOS4

<220>
<223>   This sensor binds to noscapine

<400>  2

Met Val Ala Arg Pro Lys Ser Glu Asp Lys Lys Gln Ala Leu Leu Glu 
1               5                   10                  15      


Ala Ala Thr Gln Ala Ile Ala Gln Ser Gly Ile Ala Ala Ser Thr Ala 
            20                  25                  30          


Val Ile Ala Arg Asn Ala Gly Val Ala Glu Gly Thr Leu Phe Arg Tyr 
        35                  40                  45              


Phe Ala Thr Lys Asp Glu Leu Ile Asn Thr Leu Tyr Leu His Leu Thr 
    50                  55                  60                  


His Asp Met Cys Gln Ser Leu Ile Met Glu Leu Asp Arg Ser Ile Thr 
65                  70                  75                  80  


Asp Ala Lys Met Met Thr Arg Phe Ile Trp Asn Ser Tyr Ile Ser Trp 
                85                  90                  95      


Gly Leu Asn His Pro Ala Arg His Arg Ala Ile Arg Gln Leu Ala Val 
            100                 105                 110         


Ser Glu Lys Leu Thr Lys Glu Thr Arg Gln Arg Ala Arg Asp Met Phe 
        115                 120                 125             


Pro Glu Leu Arg Asp Leu Cys Tyr Arg Ser Leu Leu Met Val Phe Met 
    130                 135                 140                 


Ser Asp Glu Tyr Arg Ala Phe Gly Asp Gly Leu Phe Met Ala Leu Ala 
145                 150                 155                 160 


Glu Thr Thr Met Asp Phe Ala Ala Arg Asp Pro Ala Arg Ala Gly Glu 
                165                 170                 175     


Tyr Ile Ala Leu Gly Phe Glu Ala Met Trp Arg Ala Leu Thr Arg Glu 
            180                 185                 190         


Glu Gln 
        


<210>  3
<211>  194
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Description of sequence: peptide sensor

<220>
<223>   Name: PAP4

<220>
<223>   This sensor binds to papaverine

<400>  3

Met Val Ala Arg Pro Lys Ser Glu Asp Lys Lys Gln Ala Leu Leu Glu 
1               5                   10                  15      


Ala Ala Thr Gln Ala Ile Ala Gln Ser Gly Ile Ala Ala Ser Thr Ala 
            20                  25                  30          


Val Ile Ala Arg Asn Ala Gly Val Ala Glu Gly Thr Leu Phe Arg Tyr 
        35                  40                  45              


Phe Ala Thr Lys Asp Glu Leu Ile Asn Thr Leu Tyr Leu His Leu Arg 
    50                  55                  60                  


Gln Asp Leu Cys Gln Ser Leu Ile Met Glu Leu Asp Arg Ser Ile Thr 
65                  70                  75                  80  


Asp Ala Lys Met Met Met Arg Phe Ile Trp Asn Ser Gly Ile Ser Trp 
                85                  90                  95      


Gly Leu Asn His Pro Ala Arg His Arg Ala Ile Arg Gln Leu Ala Val 
            100                 105                 110         


Ser Glu Lys Leu Thr Lys Glu Thr His Gln Arg Asp Leu Asp Met Phe 
        115                 120                 125             


Pro Glu Leu Arg Asp Ile Leu His Arg Arg Val Leu Met Val Phe Met 
    130                 135                 140                 


Ser Asp Glu Tyr Arg Ala Phe Gly Asp Gly Leu Phe Leu Ala Leu Ala 
145                 150                 155                 160 


Glu Thr Thr Met Asp Phe Ala Ala Arg Asp Pro Ala Arg Ala Gly Glu 
                165                 170                 175     


Tyr Ile Ala Leu Gly Phe Glu Ala Met Trp Arg Ala Leu Thr Arg Glu 
            180                 185                 190         


Glu Gln 
        


<210>  4
<211>  194
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Description of sequence: peptide sensor

<220>
<223>   Name: ROTU4

<220>
<223>   This sensor binds to rotundine

<400>  4

Met Val Ala Arg Pro Lys Ser Glu Asp Lys Lys Gln Ala Leu Leu Glu 
1               5                   10                  15      


Ala Ala Thr Gln Ala Ile Ala Gln Ser Gly Ile Ala Ala Ser Thr Ala 
            20                  25                  30          


Val Ile Ala Arg Asn Ala Gly Val Ala Glu Gly Thr Leu Phe Arg Tyr 
        35                  40                  45              


Phe Ala Thr Lys Asp Glu Leu Ile Asn Thr Leu Tyr Leu His Leu Tyr 
    50                  55                  60                  


Gln Asp His Cys Gln Ser Leu Ile Met Glu Leu Asp Arg Ser Ile Thr 
65                  70                  75                  80  


Asp Ala Lys Met Met Ile Arg Phe Thr Trp Asn Ser Tyr Ile Ser Trp 
                85                  90                  95      


Gly Leu Asn His Pro Ala Arg His Arg Ala Ile Arg Gln Leu Ala Val 
            100                 105                 110         


Ser Glu Lys Leu Thr Lys Glu Thr Lys Gln Arg Ile Glu Asp Met Phe 
        115                 120                 125             


Pro Glu Leu Arg Asp Ile Leu His Arg Ser Val Leu Met Val Phe Met 
    130                 135                 140                 


Ser Asp Glu Tyr Ser Ala Phe Gly Lys Gly Leu Phe Tyr Ala Leu Ala 
145                 150                 155                 160 


Glu Thr Thr Met Asp Phe Ala Ala Arg Asp Pro Ala Arg Ala Gly Glu 
                165                 170                 175     


Tyr Ile Ala Leu Gly Phe Glu Ala Met Trp Arg Ala Leu Thr Arg Glu 
            180                 185                 190         


Glu Gln 
        


<210>  5
<211>  194
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Description of sequence: peptide sensor

<220>
<223>   Name: THP4

<220>
<223>   This sensor binds to tetrahydropapaverine

<400>  5

Met Val Ala Arg Pro Lys Ser Glu Asp Lys Lys Gln Ala Leu Leu Glu 
1               5                   10                  15      


Ala Ala Thr Gln Ala Ile Ala Gln Ser Gly Thr Ala Ala Ser Thr Ala 
            20                  25                  30          


Val Ile Ala Arg Asn Ala Gly Val Ala Glu Gly Thr Leu Phe Arg Tyr 
        35                  40                  45              


Phe Ala Thr Lys Asp Glu Leu Ile Asn Thr Leu Tyr Leu His Leu Phe 
    50                  55                  60                  


Gln Asp Trp Cys Gln Ser Ser Ile Met Glu Leu Asp Arg Ser Ile Thr 
65                  70                  75                  80  


Asp Ala Lys Met Met Thr Arg Phe Leu Trp Asn Ser Ile Ile Ser Trp 
                85                  90                  95      


Gly Leu Asn His Pro Ala Arg His Arg Ala Ile Arg Gln Leu Ala Val 
            100                 105                 110         


Ser Glu Lys Leu Ser Lys Glu Thr Val Gln Arg Ala Asp Asp Met Phe 
        115                 120                 125             


Pro Glu Leu Arg Asp Ile Val His Arg Glu Val Leu Met Val Phe Met 
    130                 135                 140                 


Ser Asp Glu Tyr Arg Ala Phe Gly Glu Gly Leu Phe Leu Ala Leu Ala 
145                 150                 155                 160 


Glu Thr Thr Met Asp Phe Ala Ala Arg Asp Pro Ala Arg Ala Gly Glu 
                165                 170                 175     


Tyr Ile Ala Leu Gly Phe Glu Ala Met Trp Arg Ala Leu Thr Arg Glu 
            180                 185                 190         


Glu Gln 
        


<210>  6
<211>  194
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Description of sequence: peptide sensor

<220>
<223>   Name: 4NB2

<220>
<223>   This sensor binds to 4-Omethylnorbelladine

<400>  6

Met Val Ala Arg Pro Lys Ser Glu Asp Lys Lys Gln Ala Leu Leu Glu 
1               5                   10                  15      


Ala Ala Thr Gln Ala Ile Ala Gln Ser Gly Ile Ala Ala Ser Thr Ala 
            20                  25                  30          


Val Ile Ala Arg Asn Ala Gly Val Ala Glu Gly Thr Leu Phe Arg Tyr 
        35                  40                  45              


Phe Ala Thr Lys Asp Glu Leu Ile Asn Thr Leu Tyr Leu His Leu Thr 
    50                  55                  60                  


Gln Asp Met Cys Gln Ser Met Ile Met Glu Leu Asp Arg Ser Ile Thr 
65                  70                  75                  80  


Asp Ala Lys Met Met Thr Arg Phe Ile Trp Asn Ser Tyr Ile Ser Trp 
                85                  90                  95      


Gly Leu Asn His Pro Ala Arg His Arg Ala Ile Arg Gln Leu Ala Val 
            100                 105                 110         


Ser Glu Lys Leu Thr Lys Glu Thr Glu Gln Arg Ala Asp Asp Met Phe 
        115                 120                 125             


Pro Glu Leu Arg Asp Leu Asp His Arg Gly Val Leu Met Val Phe Met 
    130                 135                 140                 


Ser Asp Glu Tyr Arg Ala Phe Gly Asp Gly Leu Phe Leu Ala Leu Ala 
145                 150                 155                 160 


Glu Thr Thr Met Asp Phe Ala Ala Arg Asp Pro Ala Arg Ala Gly Glu 
                165                 170                 175     


Tyr Ile Ala Leu Gly Phe Glu Ala Met Trp Arg Ala Leu Thr Arg Glu 
            180                 185                 190         


Glu Gln 
        


<210>  7
<211>  585
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Description of sequence: encodes for peptide sensor

<220>
<223>   DNA encoding for GLAU4

<400>  7
atggttgctc gcccaaagtc tgaggacaaa aagcaggcat tgcttgaagc ggcaactcaa       60

gccatcgcgc aatcaggcat tgccgctagt accgctgtaa ttgcacgcaa tgcgggagtt      120

gcggaaggga cgttgttccg ctatttcgca acgaaagatg agttgatcaa caccctttac      180

ttacatttga cccaggactg gtgccaatca atcatcatgg aattggatcg ttctattact      240

gacgctaaga tgatgacccg ttttttgtgg aacagttgga ttagctgggg attgaaccac      300

ccagctcgcc atcgtgccat tcgtcagttg gcggtttctg aaaagttgac gaaggaaacc      360

gaacaacgcg cggatgatat gttcccggag ttacgcgacc acctgcaccg taacgttctt      420

atggtgttta tgtccgacga gtaccgcgcc ttcggcgacg ggttgttctt ggcgcttgct      480

gagacgacta tggatttcgc tgcgcgcgac ccggctcgcg ctggtgagta cattgcgttg      540

ggcttcgagg ctatgtggcg cgcattggcc cgcgaagagc agtaa                      585


<210>  8
<211>  585
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Description of sequence: encodes for peptide sensor

<220>
<223>   DNA encoding for NOS4

<400>  8
atggttgctc gcccaaagtc tgaggacaaa aagcaggcat tgcttgaagc ggcaactcaa       60

gccatcgcgc aatcaggcat tgccgctagt accgctgtaa ttgcacgcaa tgcgggagtt      120

gcggaaggga cgttgttccg ctatttcgca acgaaagatg agttgatcaa caccctttac      180

ttacatttga cccatgacat gtgccaatca ctgatcatgg aattggatcg ttctattact      240

gacgctaaga tgatgacccg ttttatctgg aacagttata ttagctgggg attgaaccac      300

ccagctcgcc atcgtgccat tcgtcagttg gcggtttctg aaaagttgac gaaggaaacc      360

cgccaacgcg cccgcgatat gttcccggag ttacgcgact tgtgctaccg tagtttgctt      420

atggtgttta tgtccgacga gtaccgcgcc ttcggcgacg ggttgttcat ggcgcttgct      480

gagacgacta tggatttcgc tgcgcgcgac ccggctcgcg ctggtgagta cattgcgttg      540

ggcttcgaag ctatgtggcg cgcacttacg cgcgaagagc agtaa                      585


<210>  9
<211>  585
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Description of sequence: encodes for peptide sensor

<220>
<223>   DNA encoding for PAP4

<400>  9
atggttgctc gcccaaagtc tgaggacaaa aagcaggcat tgcttgaagc ggcaactcaa       60

gccatcgcgc aatcaggcat tgccgctagt accgctgtaa ttgcacgcaa tgcgggagtt      120

gcggaaggga cgttgttccg ctatttcgca acgaaagatg agttgatcaa caccctttac      180

ttacatttga ggcaggacct gtgccaatca ctcatcatgg aattggatcg ttctattact      240

gacgctaaga tgatgatgcg ttttatctgg aacagtggca ttagctgggg attgaaccac      300

ccagctcgcc atcgtgccat tcgtcagttg gcggtttctg aaaagttgac gaaggaaacc      360

caccaacgcg acctggatat gttcccggag ttacgcgaca tcctgcaccg tagggttctt      420

atggtgttta tgtccgacga gtaccgcgcc ttcggcgacg ggttgttctt ggcgcttgct      480

gagacgacta tggatttcgc tgcgcgcgac ccggctcgcg ctggtgagta cattgcgttg      540

ggcttcgagg ctatgtggcg cgcacttacg cgcgaagagc agtaa                      585


<210>  10
<211>  585
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Description of sequence: encodes for peptide sensor

<220>
<223>   DNA encoding for ROTU4

<400>  10
atggttgctc gcccaaagtc tgaggacaaa aagcaggcat tgcttgaagc ggcaactcaa       60

gccatcgcgc aatcaggcat tgccgctagt accgctgtaa ttgcacgcaa tgcgggagtt      120

gcggaaggga cgttgttccg ctatttcgca acgaaagatg agttgatcaa caccctttac      180

ttacatttgt accaggacca ctgccaatca ctgatcatgg aattggatcg ttctattact      240

gacgctaaga tgatgatccg ttttacctgg aacagttaca ttagctgggg attgaaccac      300

ccagctcgcc atcgtgccat tcgtcagttg gcggtttctg aaaagttgac gaaggaaacc      360

aagcaacgca tcgaggatat gttcccggag ttacgcgaca tcctgcaccg tagtgttctt      420

atggtgttta tgtccgacga gtactccgcc ttcggcaagg ggttgttcta cgcgcttgct      480

gagacgacta tggatttcgc tgcgcgcgac ccggctcgcg ctggtgagta cattgcgttg      540

ggcttcgagg ctatgtggcg cgcacttacg cgcgaagagc agtaa                      585


<210>  11
<211>  585
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Description of sequence: encodes for peptide sensor

<220>
<223>   DNA encoding for THP4

<400>  11
atggttgctc gcccaaagtc tgaggacaaa aagcaggcat tgcttgaagc agcaactcaa       60

gccatcgcgc aatcaggcac tgccgctagt accgctgtaa ttgcacgcaa tgcgggagtt      120

gcggaaggga cgttgttccg ctatttcgca acgaaagatg agttgatcaa caccctttac      180

ttacatttgt tccaggactg gtgccaatca tccatcatgg aattggatcg ttctattact      240

gacgctaaga tgatgacgcg ttttctctgg aacagtatca ttagctgggg attaaaccac      300

ccagctcgcc atcgtgccat tcgtcagttg gcggtttctg aaaagttgtc gaaggagacc      360

gtacaacgcg cggatgatat gttcccggag ttacgcgaca tcgtccaccg tgaggttctt      420

atggtgttta tgtccgacga gtaccgcgcc ttcggcgaag ggttgttctt ggcgcttgct      480

gagacgacta tggatttcgc tgcgcgcgac ccggctcgcg ctggtgagta cattgcgttg      540

ggcttcgagg ctatgtggcg cgcacttacg cgcgaagagc agtaa                      585


<210>  12
<211>  585
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Description of sequence: encodes for peptide sensor

<220>
<223>   DNA encoding for 4NB2

<400>  12
atggttgctc gcccaaagtc tgaggacaaa aagcaggcat tgcttgaagc ggcaactcaa       60

gccatcgcgc aatcaggcat tgccgctagt accgctgtaa ttgcacgcaa tgcgggagtt      120

gcggaaggga cgttgttccg ctatttcgca acgaaagatg agttgatcaa caccctttac      180

ttacatttga cccaggacat gtgccaatca atgatcatgg aattggatcg ttctattact      240

gacgctaaga tgatgacccg ttttatctgg aacagttata ttagctgggg attgaaccac      300

ccagctcgcc atcgtgccat tcgtcagttg gcggtttctg aaaagttgac gaaggaaacc      360

gaacaacgcg cggatgatat gttcccggag ttacgcgacc tcgaccaccg tggcgttctt      420

atggtgttta tgtccgacga gtaccgcgcc ttcggcgacg ggttgttctt ggcgcttgct      480

gagacgacta tggatttcgc tgcgcgcgac ccggctcgcg ctggtgagta cattgcgttg      540

ggcttcgagg ctatgtggcg cgcacttacg cgcgaagagc agtaa                      585


<210>  13
<211>  77
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Description of sequence: GLAU variant

<220>
<223>   Name: Pbm3r1

<400>  13
ggctgtcgga cctttgaaaa gttcgttgac agctagctca gtcctaggta caatcggaat       60

gaacgttcat tccgttt                                                      77


<210>  14
<211>  61
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Description of sequence: GLAU variant

<220>
<223>   Name: Pramr

<400>  14
ctttgaaaag taccttgacg gcgtatcttt gctttctata atgagtgctt actcactcat       60

a                                                                       61


<210>  15
<211>  69
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Description of sequence: GLAU variant

<220>
<223>   Name: Pqacr

<400>  15
attcgttacc aattgacagc tagctcagtc ctactttagt atatagagac tgagcggtcg       60

gtctatata                                                               69


<210>  16
<211>  56
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Description of sequence: GLAU variant

<220>
<223>   Name: Pttgr

<400>  16
aacgcaccca gcagtattta caaacaacca tgaatgtaag tataatagtt agcaat           56


<210>  17
<211>  52
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Description of sequence: GLAU variant

<220>
<223>   Name: Pnald

<400>  17
cacctttcgg acgtttacaa acacctatga atgtaagtat aatagttagc aa               52


<210>  18
<211>  52
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Description of sequence: GLAU variant

<220>
<223>   Name: Psmet

<400>  18
cacccagcag tatttacaaa caaacaagca tgtatgtata atccttagca ac               52


