<110>    Seoul National University R and DB Foundation

<120>    Transgenic animal in which APEX2 is expressed specifically for
         mitochondrial matrix and use thereof

<130>    1069432

<150>    KR 1020210084274
<151>    2021-06-28

<160>    6

<170>    KoPatentIn 3.0

<210>    1
<211>    24
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    MTS


<400>    1
Met Leu Ala Thr Arg Val Phe Ser Leu Val Gly Lys Arg Ala Ile Ser
  1               5                  10                  15 

Thr Ser Val Cys Val Arg Ala His
             20                


<210>    2
<211>    14
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    V5


<400>    2
Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr
  1               5                  10                


<210>    3
<211>    249
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    APEX2


<400>    3
Gly Lys Ser Tyr Pro Thr Val Ser Ala Asp Tyr Gln Asp Ala Val Glu
  1               5                  10                  15 

Lys Ala Lys Lys Lys Leu Arg Gly Phe Ile Ala Glu Lys Arg Cys Ala
             20                  25                  30 

Pro Leu Met Leu Arg Leu Ala Phe His Ser Ala Gly Thr Phe Asp Lys
         35                  40                  45 

Gly Thr Lys Thr Gly Gly Pro Phe Gly Thr Ile Lys His Pro Ala Glu
     50                  55                  60 

Leu Ala His Ser Ala Asn Asn Gly Leu Asp Ile Ala Val Arg Leu Leu
 65                  70                  75                  80 

Glu Pro Leu Lys Ala Glu Phe Pro Ile Leu Ser Tyr Ala Asp Phe Tyr
                 85                  90                  95 

Gln Leu Ala Gly Val Val Ala Val Glu Val Thr Gly Gly Pro Lys Val
            100                 105                 110 

Pro Phe His Pro Gly Arg Glu Asp Lys Pro Glu Pro Pro Pro Glu Gly
        115                 120                 125 

Arg Leu Pro Asp Pro Thr Lys Gly Ser Asp His Leu Arg Asp Val Phe
    130                 135                 140 

Gly Lys Ala Met Gly Leu Thr Asp Gln Asp Ile Val Ala Leu Ser Gly
145                 150                 155                 160 

Gly His Thr Ile Gly Ala Ala His Lys Glu Arg Ser Gly Phe Glu Gly
                165                 170                 175 

Pro Trp Thr Ser Asn Pro Leu Ile Phe Asp Asn Ser Tyr Phe Thr Glu
            180                 185                 190 

Leu Leu Ser Gly Glu Lys Glu Gly Leu Leu Gln Leu Pro Ser Asp Lys
        195                 200                 205 

Ala Leu Leu Ser Asp Pro Val Phe Arg Pro Leu Val Asp Lys Tyr Ala
    210                 215                 220 

Ala Asp Glu Asp Ala Phe Phe Ala Asp Tyr Ala Glu Ala His Gln Lys
225                 230                 235                 240 

Leu Ser Glu Leu Gly Phe Ala Asp Ala
                245                


<210>    4
<211>    290
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    MTS-V5-APEX2


<400>    4
Met Leu Ala Thr Arg Val Phe Ser Leu Val Gly Lys Arg Ala Ile Ser
  1               5                  10                  15 

Thr Ser Val Cys Val Arg Ala His Lys Asp Pro Gly Lys Pro Ile Pro
             20                  25                  30 

Asn Pro Leu Leu Gly Leu Asp Ser Thr Gly Lys Ser Tyr Pro Thr Val
         35                  40                  45 

Ser Ala Asp Tyr Gln Asp Ala Val Glu Lys Ala Lys Lys Lys Leu Arg
     50                  55                  60 

Gly Phe Ile Ala Glu Lys Arg Cys Ala Pro Leu Met Leu Arg Leu Ala
 65                  70                  75                  80 

Phe His Ser Ala Gly Thr Phe Asp Lys Gly Thr Lys Thr Gly Gly Pro
                 85                  90                  95 

Phe Gly Thr Ile Lys His Pro Ala Glu Leu Ala His Ser Ala Asn Asn
            100                 105                 110 

Gly Leu Asp Ile Ala Val Arg Leu Leu Glu Pro Leu Lys Ala Glu Phe
        115                 120                 125 

Pro Ile Leu Ser Tyr Ala Asp Phe Tyr Gln Leu Ala Gly Val Val Ala
    130                 135                 140 

Val Glu Val Thr Gly Gly Pro Lys Val Pro Phe His Pro Gly Arg Glu
145                 150                 155                 160 

Asp Lys Pro Glu Pro Pro Pro Glu Gly Arg Leu Pro Asp Pro Thr Lys
                165                 170                 175 

Gly Ser Asp His Leu Arg Asp Val Phe Gly Lys Ala Met Gly Leu Thr
            180                 185                 190 

Asp Gln Asp Ile Val Ala Leu Ser Gly Gly His Thr Ile Gly Ala Ala
        195                 200                 205 

His Lys Glu Arg Ser Gly Phe Glu Gly Pro Trp Thr Ser Asn Pro Leu
    210                 215                 220 

Ile Phe Asp Asn Ser Tyr Phe Thr Glu Leu Leu Ser Gly Glu Lys Glu
225                 230                 235                 240 

Gly Leu Leu Gln Leu Pro Ser Asp Lys Ala Leu Leu Ser Asp Pro Val
                245                 250                 255 

Phe Arg Pro Leu Val Asp Lys Tyr Ala Ala Asp Glu Asp Ala Phe Phe
            260                 265                 270 

Ala Asp Tyr Ala Glu Ala His Gln Lys Leu Ser Glu Leu Gly Phe Ala
        275                 280                 285 

Asp Ala
    290


<210>    5
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    forward primer


<400>    5
gtcgacgagc tcgtttagtg a                                                   21


<210>    6
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    reverse primer


<400>    6
aagaccgttg ttagcgctgt g                                                   21
