                         SEQUENCE LISTING

<110>  FUNDACIN MIGUEL SERVET
       UNIVERSIDAD PBLICA DE NAVARRA
 
<120>  SARS-CoV-2 peptides and proteins with reduced adherence to 
       Angiotensin Converting Enzyme-2

<130>  P5710PC00

<150>  EP21382574
<151>  2021-06-29

<160>  189   

<170>  PatentIn version 3.5

<210>  1
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  1

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  2
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  2

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Trp Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  3
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  3

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Trp Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  4
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  4

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  5
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  5

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  6
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  6

Ala Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  7
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  7

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Asn Gly Val Gly Ala 
    50                  55          


<210>  8
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  8

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Asn Gly Val Gly Ala 
    50                  55          


<210>  9
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  9

Tyr Asn Tyr Leu Ala Arg Ala Ala Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  10
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  10

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Asn Gly Val Gly Ala 
    50                  55          


<210>  11
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  11

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Trp Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Tyr Gly Val Gly Tyr 
    50                  55          


<210>  12
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  12

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Trp Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Tyr Gly Val Gly Tyr 
    50                  55          


<210>  13
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  13

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Tyr Gly Val Gly Tyr 
    50                  55          


<210>  14
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  14

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Tyr Gly Val Gly Tyr 
    50                  55          


<210>  15
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  15

Ala Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Tyr Gly Val Gly Tyr 
    50                  55          


<210>  16
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  16

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Tyr Gly Val Gly Ala 
    50                  55          


<210>  17
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  17

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Tyr Gly Val Gly Ala 
    50                  55          


<210>  18
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  18

Tyr Asn Tyr Leu Ala Arg Ala Ala Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Tyr Gly Val Gly Tyr 
    50                  55          


<210>  19
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  19

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Tyr Gly Val Gly Ala 
    50                  55          


<210>  20
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  20

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Trp Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Tyr Gly Val Gly Tyr 
    50                  55          


<210>  21
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  21

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Lys Gly Phe Trp Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Tyr Gly Val Gly Tyr 
    50                  55          


<210>  22
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  22

<400>  22

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Lys Gly Phe Asn Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Tyr Gly Val Gly Tyr 
    50                  55          


<210>  23
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  23

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Lys Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Tyr Gly Val Gly Tyr 
    50                  55          


<210>  24
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  24

<400>  24

Ala Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Lys Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Tyr Gly Val Gly Tyr 
    50                  55          


<210>  25
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  25

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Tyr Gly Val Gly Ala 
    50                  55          


<210>  26
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  26

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Tyr Gly Val Gly Ala 
    50                  55          


<210>  27
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  27

Tyr Asn Tyr Leu Ala Arg Ala Ala Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Tyr Gly Val Gly Tyr 
    50                  55          


<210>  28
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  28

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Lys Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Tyr Gly Val Gly Ala 
    50                  55          


<210>  29
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  29

Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Trp Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  30
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  30

Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Phe Trp Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  31
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  31

Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Phe Asn Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  32
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  32

Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  33
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  33

Ala Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  34
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  34

Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Asn Gly Val Gly Ala 
    50                  55          


<210>  35
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  35

Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Asn Gly Val Gly Ala 
    50                  55          


<210>  36
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  36

Tyr Asn Tyr Arg Ala Arg Ala Ala Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  37
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  37

Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Asn Gly Val Gly Ala 
    50                  55          


<210>  38
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  38

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Trp Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  39
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  39

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Phe Trp Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  40
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  40

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Phe Asn Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  41
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  41

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  42
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  42

Ala Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  43
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  43

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Asn Gly Val Gly Ala 
    50                  55          


<210>  44
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  44

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Asn Gly Val Gly Ala 
    50                  55          


<210>  45
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  45

Tyr Asn Tyr Leu Ala Arg Ala Ala Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  46
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  46

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
            20                  25                  30          


Asn Gly Val Gln Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Asn Gly Val Gly Ala 
    50                  55          


<210>  47
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD wild type

<400>  47

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             


<210>  48
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD wild type

<400>  48
agggtccagc caacagagtc tattgtccgc ttcccaaaca tcacaaattt gtgtcctttc       60

ggtgaggttt tcaatgctac aaggtttgca tctgtgtacg cctggaaccg caagcgtatc      120

tccaactgcg tggcagacta ctctgtactt tataattctg cttctttttc aacctttaaa      180

tgctatggag tatcgcctac taaactgaac gatttgtgtt ttaccaacgt gtatgcagac      240

tcattcgtga taaggggcga tgaggtacgc caaatcgctc ccggacagac cggcaagatt      300

gcggattaca actacaagct cccagatgac ttcacgggct gcgtaatcgc atggaattct      360

aataaccttg atagtaaggt cggtggtaat tataattatc tgtatcgtct gtttcgtaaa      420

tccaacctga aaccgtttga aagagatatt tcgaccgaga tatatcaagc gggctcaacc      480

ccatgcaacg gtgttgaagg cttcaattgt tactttccgc tccagagtta cggttttcag      540

cccacgaatg gagtaggata ccaaccctac cgtgttgtgg tgctgagttt tgaactgctg      600

cacgcaccgg caactgtgtg cggtcccaag aagtcaacaa atcttgtaaa aaataagtgt      660

gttaattttt ga                                                          672


<210>  49
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD A475W

<400>  49

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Trp Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             


<210>  50
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD A475W

<400>  50
agggtccagc caacagagtc tattgtccgc ttcccaaaca tcacaaattt gtgtcctttc       60

ggtgaggttt tcaatgctac aaggtttgca tctgtgtacg cctggaaccg caagcgtatc      120

tccaactgcg tggcagacta ctctgtactt tataattctg cttctttttc aacctttaaa      180

tgctatggag tatcgcctac taaactgaac gatttgtgtt ttaccaacgt gtatgcagac      240

tcattcgtga taaggggcga tgaggtacgc caaatcgctc ccggacagac cggcaagatt      300

gcggattaca actacaagct cccagatgac ttcacgggct gcgtaatcgc atggaattct      360

aataaccttg atagtaaggt cggtggtaat tataattatc tgtatcgtct gtttcgtaaa      420

tccaacctga aaccgtttga aagagatatt tcgaccgaga tatatcaatg gggctcaacc      480

ccatgcaacg gtgttgaagg cttcaattgt tactttccgc tccagagtta cggttttcag      540

cccacgaatg gagtaggata ccaaccctac cgtgttgtgg tgctgagttt tgaactgctg      600

cacgcaccgg caactgtgtg cggtcccaag aagtcaacaa atcttgtaaa aaataagtgt      660

gttaattttt ga                                                          672


<210>  51
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD N487W

<400>  51

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Trp Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             


<210>  52
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD N487W

<400>  52
agggtccagc caacagagtc tattgtccgc ttcccaaaca tcacaaattt gtgtcctttc       60

ggtgaggttt tcaatgctac aaggtttgca tctgtgtacg cctggaaccg caagcgtatc      120

tccaactgcg tggcagacta ctctgtactt tataattctg cttctttttc aacctttaaa      180

tgctatggag tatcgcctac taaactgaac gatttgtgtt ttaccaacgt gtatgcagac      240

tcattcgtga taaggggcga tgaggtacgc caaatcgctc ccggacagac cggcaagatt      300

gcggattaca actacaagct cccagatgac ttcacgggct gcgtaatcgc atggaattct      360

aataaccttg atagtaaggt cggtggtaat tataattatc tgtatcgtct gtttcgtaaa      420

tccaacctga aaccgtttga aagagatatt tcgaccgaga tatatcaagc gggctcaacc      480

ccatgcaacg gtgttgaagg cttctggtgt tactttccgc tccagagtta cggttttcag      540

cccacgaatg gagtaggata ccaaccctac cgtgttgtgg tgctgagttt tgaactgctg      600

cacgcaccgg caactgtgtg cggtcccaag aagtcaacaa atcttgtaaa aaataagtgt      660

gttaattttt ga                                                          672


<210>  53
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD Y489A

<400>  53

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Ala Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             


<210>  54
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD Y489A

<400>  54
agggtccagc caacagagtc tattgtccgc ttcccaaaca tcacaaattt gtgtcctttc       60

ggtgaggttt tcaatgctac aaggtttgca tctgtgtacg cctggaaccg caagcgtatc      120

tccaactgcg tggcagacta ctctgtactt tataattctg cttctttttc aacctttaaa      180

tgctatggag tatcgcctac taaactgaac gatttgtgtt ttaccaacgt gtatgcagac      240

tcattcgtga taaggggcga tgaggtacgc caaatcgctc ccggacagac cggcaagatt      300

gcggattaca actacaagct cccagatgac ttcacgggct gcgtaatcgc atggaattct      360

aataaccttg atagtaaggt cggtggtaat tataattatc tgtatcgtct gtttcgtaaa      420

tccaacctga aaccgtttga aagagatatt tcgaccgaga tatatcaagc gggctcaacc      480

ccatgcaacg gtgttgaagg cttcaattgt gcgtttccgc tccagagtta cggttttcag      540

cccacgaatg gagtaggata ccaaccctac cgtgttgtgg tgctgagttt tgaactgctg      600

cacgcaccgg caactgtgtg cggtcccaag aagtcaacaa atcttgtaaa aaataagtgt      660

gttaattttt ga                                                          672


<210>  55
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD F486A/N487A/Y489A

<400>  55

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             


<210>  56
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD F486A/N487A/Y489A

<400>  56
agggtccagc caacagagtc tattgtccgc ttcccaaaca tcacaaattt gtgtcctttc       60

ggtgaggttt tcaatgctac aaggtttgca tctgtgtacg cctggaaccg caagcgtatc      120

tccaactgcg tggcagacta ctctgtactt tataattctg cttctttttc aacctttaaa      180

tgctatggag tatcgcctac taaactgaac gatttgtgtt ttaccaacgt gtatgcagac      240

tcattcgtga taaggggcga tgaggtacgc caaatcgctc ccggacagac cggcaagatt      300

gcggattaca actacaagct cccagatgac ttcacgggct gcgtaatcgc atggaattct      360

aataaccttg atagtaaggt cggtggtaat tataattatc tgtatcgtct gtttcgtaaa      420

tccaacctga aaccgtttga aagagatatt tcgaccgaga tatatcaagc gggctcaacc      480

ccatgcaacg gtgttgaagg cgcggcgtgt gcgtttccgc tccagagtta cggttttcag      540

cccacgaatg gagtaggata ccaaccctac cgtgttgtgg tgctgagttt tgaactgctg      600

cacgcaccgg caactgtgtg cggtcccaag aagtcaacaa atcttgtaaa aaataagtgt      660

gttaattttt ga                                                          672


<210>  57
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD Y449A/F486A/N487A/Y489A

<400>  57

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Ala Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             


<210>  58
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD Y449A/F486A/N487A/Y489A

<400>  58
agggtccagc caacagagtc tattgtccgc ttcccaaaca tcacaaattt gtgtcctttc       60

ggtgaggttt tcaatgctac aaggtttgca tctgtgtacg cctggaaccg caagcgtatc      120

tccaactgcg tggcagacta ctctgtactt tataattctg cttctttttc aacctttaaa      180

tgctatggag tatcgcctac taaactgaac gatttgtgtt ttaccaacgt gtatgcagac      240

tcattcgtga taaggggcga tgaggtacgc caaatcgctc ccggacagac cggcaagatt      300

gcggattaca actacaagct cccagatgac ttcacgggct gcgtaatcgc atggaattct      360

aataaccttg atagtaaggt cggtggtaat gcgaattatc tgtatcgtct gtttcgtaaa      420

tccaacctga aaccgtttga aagagatatt tcgaccgaga tatatcaagc gggctcaacc      480

ccatgcaacg gtgttgaagg cgcggcgtgt gcgtttccgc tccagagtta cggttttcag      540

cccacgaatg gagtaggata ccaaccctac cgtgttgtgg tgctgagttt tgaactgctg      600

cacgcaccgg caactgtgtg cggtcccaag aagtcaacaa atcttgtaaa aaataagtgt      660

gttaattttt ga                                                          672


<210>  59
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD Q498A/T500A/Y505A

<400>  59

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Ala Pro Ala Asn Gly Val Gly Ala Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             


<210>  60
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD Q498A/T500A/Y505A

<400>  60
agggtccagc caacagagtc tattgtccgc ttcccaaaca tcacaaattt gtgtcctttc       60

ggtgaggttt tcaatgctac aaggtttgca tctgtgtacg cctggaaccg caagcgtatc      120

tccaactgcg tggcagacta ctctgtactt tataattctg cttctttttc aacctttaaa      180

tgctatggag tatcgcctac taaactgaac gatttgtgtt ttaccaacgt gtatgcagac      240

tcattcgtga taaggggcga tgaggtacgc caaatcgctc ccggacagac cggcaagatt      300

gcggattaca actacaagct cccagatgac ttcacgggct gcgtaatcgc atggaattct      360

aataaccttg atagtaaggt cggtggtaat tataattatc tgtatcgtct gtttcgtaaa      420

tccaacctga aaccgtttga aagagatatt tcgaccgaga tatatcaagc gggctcaacc      480

ccatgcaacg gtgttgaagg cttcaattgt tactttccgc tccagagtta cggttttgcg      540

cccgcgaatg gagtaggagc gcaaccctac cgtgttgtgg tgctgagttt tgaactgctg      600

cacgcaccgg caactgtgtg cggtcccaag aagtcaacaa atcttgtaaa aaataagtgt      660

gttaattttt ga                                                          672


<210>  61
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD Q493A/Q498A/T500A/Y505

<400>  61

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser 
                165                 170                 175     


Tyr Gly Phe Ala Pro Ala Asn Gly Val Gly Ala Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             


<210>  62
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD Q493A/Q498A/T500A/Y505

<400>  62
agggtccagc caacagagtc tattgtccgc ttcccaaaca tcacaaattt gtgtcctttc       60

ggtgaggttt tcaatgctac aaggtttgca tctgtgtacg cctggaaccg caagcgtatc      120

tccaactgcg tggcagacta ctctgtactt tataattctg cttctttttc aacctttaaa      180

tgctatggag tatcgcctac taaactgaac gatttgtgtt ttaccaacgt gtatgcagac      240

tcattcgtga taaggggcga tgaggtacgc caaatcgctc ccggacagac cggcaagatt      300

gcggattaca actacaagct cccagatgac ttcacgggct gcgtaatcgc atggaattct      360

aataaccttg atagtaaggt cggtggtaat tataattatc tgtatcgtct gtttcgtaaa      420

tccaacctga aaccgtttga aagagatatt tcgaccgaga tatatcaagc gggctcaacc      480

ccatgcaacg gtgttgaagg cttcaattgt tactttccgc tcgcgagtta cggttttgcg      540

cccgcgaatg gagtaggagc gcaaccctac cgtgttgtgg tgctgagttt tgaactgctg      600

cacgcaccgg caactgtgtg cggtcccaag aagtcaacaa atcttgtaaa aaataagtgt      660

gttaattttt ga                                                          672


<210>  63
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD Y453A/L455A/F456A/Q493A

<400>  63

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Ala Arg Ala Ala Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             


<210>  64
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD Y453A/L455A/F456A/Q493A

<400>  64
agggtccagc caacagagtc tattgtccgc ttcccaaaca tcacaaattt gtgtcctttc       60

ggtgaggttt tcaatgctac aaggtttgca tctgtgtacg cctggaaccg caagcgtatc      120

tccaactgcg tggcagacta ctctgtactt tataattctg cttctttttc aacctttaaa      180

tgctatggag tatcgcctac taaactgaac gatttgtgtt ttaccaacgt gtatgcagac      240

tcattcgtga taaggggcga tgaggtacgc caaatcgctc ccggacagac cggcaagatt      300

gcggattaca actacaagct cccagatgac ttcacgggct gcgtaatcgc atggaattct      360

aataaccttg atagtaaggt cggtggtaat tataattatc tggcgcgtgc ggcgcgtaaa      420

tccaacctga aaccgtttga aagagatatt tcgaccgaga tatatcaagc gggctcaacc      480

ccatgcaacg gtgttgaagg cttcaattgt tactttccgc tcgcgagtta cggttttcag      540

cccacgaatg gagtaggata ccaaccctac cgtgttgtgg tgctgagttt tgaactgctg      600

cacgcaccgg caactgtgtg cggtcccaag aagtcaacaa atcttgtaaa aaataagtgt      660

gttaattttt ga                                                          672


<210>  65
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD F486A/N487A/Y489A/Q498A/T500A/Y505A

<400>  65

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Ala Pro Ala Asn Gly Val Gly Ala Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             


<210>  66
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD F486A/N487A/Y489A/Q498A/T500A/Y505A

<400>  66
agggtccagc caacagagtc tattgtccgc ttcccaaaca tcacaaattt gtgtcctttc       60

ggtgaggttt tcaatgctac aaggtttgca tctgtgtacg cctggaaccg caagcgtatc      120

tccaactgcg tggcagacta ctctgtactt tataattctg cttctttttc aacctttaaa      180

tgctatggag tatcgcctac taaactgaac gatttgtgtt ttaccaacgt gtatgcagac      240

tcattcgtga taaggggcga tgaggtacgc caaatcgctc ccggacagac cggcaagatt      300

gcggattaca actacaagct cccagatgac ttcacgggct gcgtaatcgc atggaattct      360

aataaccttg atagtaaggt cggtggtaat tataattatc tgtatcgtct gtttcgtaaa      420

tccaacctga aaccgtttga aagagatatt tcgaccgaga tatatcaagc gggctcaacc      480

ccatgcaacg gtgttgaagg cgcggcgtgt gcgtttccgc tccagagtta cggttttgcg      540

cccgcgaatg gagtaggagc gcaaccctac cgtgttgtgg tgctgagttt tgaactgctg      600

cacgcaccgg caactgtgtg cggtcccaag aagtcaacaa atcttgtaaa aaataagtgt      660

gttaattttt ga                                                          672


<210>  67
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD F486A

<400>  67

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Ala Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             


<210>  68
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD F486A

<400>  68
agggtccagc caacagagtc tattgtccgc ttcccaaaca tcacaaattt gtgtcctttc       60

ggtgaggttt tcaatgctac aaggtttgca tctgtgtacg cctggaaccg caagcgtatc      120

tccaactgcg tggcagacta ctctgtactt tataattctg cttctttttc aacctttaaa      180

tgctatggag tatcgcctac taaactgaac gatttgtgtt ttaccaacgt gtatgcagac      240

tcattcgtga taaggggcga tgaggtacgc caaatcgctc ccggacagac cggcaagatt      300

gcggattaca actacaagct cccagatgac ttcacgggct gcgtaatcgc atggaattct      360

aataaccttg atagtaaggt cggtggtaat tataattatc tgtatcgtct gtttcgtaaa      420

tccaacctga aaccgtttga aagagatatt tcgaccgaga tatatcaagc gggctcaacc      480

ccatgcaacg gtgttgaagg cgcgaattgt tactttccgc tccagagtta cggttttcag      540

cccacgaatg gagtaggata ccaaccctac cgtgttgtgg tgctgagttt tgaactgctg      600

cacgcaccgg caactgtgtg cggtcccaag aagtcaacaa atcttgtaaa aaataagtgt      660

gttaattttt ga                                                          672


<210>  69
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD N487A

<400>  69

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Ala Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             


<210>  70
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD N487A

<400>  70
agggtccagc caacagagtc tattgtccgc ttcccaaaca tcacaaattt gtgtcctttc       60

ggtgaggttt tcaatgctac aaggtttgca tctgtgtacg cctggaaccg caagcgtatc      120

tccaactgcg tggcagacta ctctgtactt tataattctg cttctttttc aacctttaaa      180

tgctatggag tatcgcctac taaactgaac gatttgtgtt ttaccaacgt gtatgcagac      240

tcattcgtga taaggggcga tgaggtacgc caaatcgctc ccggacagac cggcaagatt      300

gcggattaca actacaagct cccagatgac ttcacgggct gcgtaatcgc atggaattct      360

aataaccttg atagtaaggt cggtggtaat tataattatc tgtatcgtct gtttcgtaaa      420

tccaacctga aaccgtttga aagagatatt tcgaccgaga tatatcaagc gggctcaacc      480

ccatgcaacg gtgttgaagg cttcgcgtgt tactttccgc tccagagtta cggttttcag      540

cccacgaatg gagtaggata ccaaccctac cgtgttgtgg tgctgagttt tgaactgctg      600

cacgcaccgg caactgtgtg cggtcccaag aagtcaacaa atcttgtaaa aaataagtgt      660

gttaattttt ga                                                          672


<210>  71
<211>  258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD wild type

<400>  71

Ala Asp Pro Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Ser 
            20                  25                  30          


Arg Gly Gly Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn 
        35                  40                  45              


Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
    50                  55                  60                  


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
65                  70                  75                  80  


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
                85                  90                  95      


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
            100                 105                 110         


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
        115                 120                 125             


Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
    130                 135                 140                 


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
145                 150                 155                 160 


Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
                165                 170                 175     


Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala 
            180                 185                 190         


Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro 
        195                 200                 205             


Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro 
    210                 215                 220                 


Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr 
225                 230                 235                 240 


Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val 
                245                 250                 255     


Asn Phe 
        


<210>  72
<211>  777
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD WT tag

<400>  72
gcggatccct ggtctcatcc gcagtttgaa aagggaggtg gaagtggcgg tggttcgggc       60

ggatcggctt ggtcgcatcc ccaattcgaa aaatcaagag gcggcagggt ccagccaaca      120

gagtctattg tccgcttccc aaacatcaca aatttgtgtc ctttcggtga ggttttcaat      180

gctacaaggt ttgcatctgt gtacgcctgg aaccgcaagc gtatctccaa ctgcgtggca      240

gactactctg tactttataa ttctgcttct ttttcaacct ttaaatgcta tggagtatcg      300

cctactaaac tgaacgattt gtgttttacc aacgtgtatg cagactcatt cgtgataagg      360

ggcgatgagg tacgccaaat cgctcccgga cagaccggca agattgcgga ttacaactac      420

aagctcccag atgacttcac gggctgcgta atcgcatgga attctaataa ccttgatagt      480

aaggtcggtg gtaattataa ttatctgtat cgtctgtttc gtaaatccaa cctgaaaccg      540

tttgaaagag atatttcgac cgagatatat caagcgggct caaccccatg caacggtgtt      600

gaaggcttca attgttactt tccgctccag agttacggtt ttcagcccac gaatggagta      660

ggataccaac cctaccgtgt tgtggtgctg agttttgaac tgctgcacgc accggcaact      720

gtgtgcggtc ccaagaagtc aacaaatctt gtaaaaaata agtgtgttaa tttttga         777


<210>  73
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD A475W

<400>  73

Ala Asp Pro Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Ser 
            20                  25                  30          


Arg Gly Gly Leu Glu Val Leu Phe Gln Gly Pro Arg Val Gln Pro Thr 
        35                  40                  45              


Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
    50                  55                  60                  


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
65                  70                  75                  80  


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
                85                  90                  95      


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
            100                 105                 110         


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
        115                 120                 125             


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala 
    130                 135                 140                 


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
145                 150                 155                 160 


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
                165                 170                 175     


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
            180                 185                 190         


Ile Ser Thr Glu Ile Tyr Gln Trp Gly Ser Thr Pro Cys Asn Gly Val 
        195                 200                 205             


Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
    210                 215                 220                 


Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
225                 230                 235                 240 


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
                245                 250                 255     


Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
            260                 265     


<210>  74
<211>  801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD A475W

<400>  74
gcggatccct ggtctcatcc gcagtttgaa aagggaggtg gaagtggcgg tggttcgggc       60

ggatcggctt ggtcgcatcc ccaattcgaa aaatcaagag gcggcctgga ggttctgttt      120

caaggtccaa gggtccagcc aacagagtct attgtccgct tcccaaacat cacaaatttg      180

tgtcctttcg gtgaggtttt caatgctaca aggtttgcat ctgtgtacgc ctggaaccgc      240

aagcgtatct ccaactgcgt ggcagactac tctgtacttt ataattctgc ttctttttca      300

acctttaaat gctatggagt atcgcctact aaactgaacg atttgtgttt taccaacgtg      360

tatgcagact cattcgtgat aaggggcgat gaggtacgcc aaatcgctcc cggacagacc      420

ggcaagattg cggattacaa ctacaagctc ccagatgact tcacgggctg cgtaatcgca      480

tggaattcta ataaccttga tagtaaggtc ggtggtaatt ataattatct gtatcgtctg      540

tttcgtaaat ccaacctgaa accgtttgaa agagatattt cgaccgagat atatcaatgg      600

ggctcaaccc catgcaacgg tgttgaaggc ttcaattgtt actttccgct ccagagttac      660

ggttttcagc ccacgaatgg agtaggatac caaccctacc gtgttgtggt gctgagtttt      720

gaactgctgc acgcaccggc aactgtgtgc ggtcccaaga agtcaacaaa tcttgtaaaa      780

aataagtgtg ttaatttttg a                                                801


<210>  75
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD N487W

<400>  75

Ala Asp Pro Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Ser 
            20                  25                  30          


Arg Gly Gly Leu Glu Val Leu Phe Gln Gly Pro Arg Val Gln Pro Thr 
        35                  40                  45              


Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
    50                  55                  60                  


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
65                  70                  75                  80  


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
                85                  90                  95      


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
            100                 105                 110         


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
        115                 120                 125             


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala 
    130                 135                 140                 


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
145                 150                 155                 160 


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
                165                 170                 175     


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
            180                 185                 190         


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
        195                 200                 205             


Glu Gly Phe Trp Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
    210                 215                 220                 


Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
225                 230                 235                 240 


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
                245                 250                 255     


Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
            260                 265     


<210>  76
<211>  801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD N487W

<400>  76
gcggatccct ggtctcatcc gcagtttgaa aagggaggtg gaagtggcgg tggttcgggc       60

ggatcggctt ggtcgcatcc ccaattcgaa aaatcaagag gcggcctgga ggttctgttt      120

caaggtccaa gggtccagcc aacagagtct attgtccgct tcccaaacat cacaaatttg      180

tgtcctttcg gtgaggtttt caatgctaca aggtttgcat ctgtgtacgc ctggaaccgc      240

aagcgtatct ccaactgcgt ggcagactac tctgtacttt ataattctgc ttctttttca      300

acctttaaat gctatggagt atcgcctact aaactgaacg atttgtgttt taccaacgtg      360

tatgcagact cattcgtgat aaggggcgat gaggtacgcc aaatcgctcc cggacagacc      420

ggcaagattg cggattacaa ctacaagctc ccagatgact tcacgggctg cgtaatcgca      480

tggaattcta ataaccttga tagtaaggtc ggtggtaatt ataattatct gtatcgtctg      540

tttcgtaaat ccaacctgaa accgtttgaa agagatattt cgaccgagat atatcaagcg      600

ggctcaaccc catgcaacgg tgttgaaggc ttctggtgtt actttccgct ccagagttac      660

ggttttcagc ccacgaatgg agtaggatac caaccctacc gtgttgtggt gctgagtttt      720

gaactgctgc acgcaccggc aactgtgtgc ggtcccaaga agtcaacaaa tcttgtaaaa      780

aataagtgtg ttaatttttg a                                                801


<210>  77
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD Y489A

<400>  77

Ala Asp Pro Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Ser 
            20                  25                  30          


Arg Gly Gly Leu Glu Val Leu Phe Gln Gly Pro Arg Val Gln Pro Thr 
        35                  40                  45              


Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
    50                  55                  60                  


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
65                  70                  75                  80  


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
                85                  90                  95      


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
            100                 105                 110         


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
        115                 120                 125             


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala 
    130                 135                 140                 


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
145                 150                 155                 160 


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
                165                 170                 175     


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
            180                 185                 190         


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
        195                 200                 205             


Glu Gly Phe Asn Cys Ala Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
    210                 215                 220                 


Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
225                 230                 235                 240 


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
                245                 250                 255     


Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
            260                 265     


<210>  78
<211>  801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD Y489A

<400>  78
gcggatccct ggtctcatcc gcagtttgaa aagggaggtg gaagtggcgg tggttcgggc       60

ggatcggctt ggtcgcatcc ccaattcgaa aaatcaagag gcggcctgga ggttctgttt      120

caaggtccaa gggtccagcc aacagagtct attgtccgct tcccaaacat cacaaatttg      180

tgtcctttcg gtgaggtttt caatgctaca aggtttgcat ctgtgtacgc ctggaaccgc      240

aagcgtatct ccaactgcgt ggcagactac tctgtacttt ataattctgc ttctttttca      300

acctttaaat gctatggagt atcgcctact aaactgaacg atttgtgttt taccaacgtg      360

tatgcagact cattcgtgat aaggggcgat gaggtacgcc aaatcgctcc cggacagacc      420

ggcaagattg cggattacaa ctacaagctc ccagatgact tcacgggctg cgtaatcgca      480

tggaattcta ataaccttga tagtaaggtc ggtggtaatt ataattatct gtatcgtctg      540

tttcgtaaat ccaacctgaa accgtttgaa agagatattt cgaccgagat atatcaagcg      600

ggctcaaccc catgcaacgg tgttgaaggc ttcaattgtg cgtttccgct ccagagttac      660

ggttttcagc ccacgaatgg agtaggatac caaccctacc gtgttgtggt gctgagtttt      720

gaactgctgc acgcaccggc aactgtgtgc ggtcccaaga agtcaacaaa tcttgtaaaa      780

aataagtgtg ttaatttttg a                                                801


<210>  79
<211>  258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD F486A/N487A/Y489A

<400>  79

Ala Asp Pro Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Ser 
            20                  25                  30          


Arg Gly Gly Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn 
        35                  40                  45              


Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
    50                  55                  60                  


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
65                  70                  75                  80  


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
                85                  90                  95      


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
            100                 105                 110         


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
        115                 120                 125             


Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
    130                 135                 140                 


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
145                 150                 155                 160 


Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
                165                 170                 175     


Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala 
            180                 185                 190         


Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro 
        195                 200                 205             


Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro 
    210                 215                 220                 


Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr 
225                 230                 235                 240 


Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val 
                245                 250                 255     


Asn Phe 
        


<210>  80
<211>  777
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD F486A/N487A/Y489A

<400>  80
gcggatccct ggtctcatcc gcagtttgaa aagggaggtg gaagtggcgg tggttcgggc       60

ggatcggctt ggtcgcatcc ccaattcgaa aaatcaagag gcggcagggt ccagccaaca      120

gagtctattg tccgcttccc aaacatcaca aatttgtgtc ctttcggtga ggttttcaat      180

gctacaaggt ttgcatctgt gtacgcctgg aaccgcaagc gtatctccaa ctgcgtggca      240

gactactctg tactttataa ttctgcttct ttttcaacct ttaaatgcta tggagtatcg      300

cctactaaac tgaacgattt gtgttttacc aacgtgtatg cagactcatt cgtgataagg      360

ggcgatgagg tacgccaaat cgctcccgga cagaccggca agattgcgga ttacaactac      420

aagctcccag atgacttcac gggctgcgta atcgcatgga attctaataa ccttgatagt      480

aaggtcggtg gtaattataa ttatctgtat cgtctgtttc gtaaatccaa cctgaaaccg      540

tttgaaagag atatttcgac cgagatatat caagcgggct caaccccatg caacggtgtt      600

gaaggcgcgg cgtgtgcgtt tccgctccag agttacggtt ttcagcccac gaatggagta      660

ggataccaac cctaccgtgt tgtggtgctg agttttgaac tgctgcacgc accggcaact      720

gtgtgcggtc ccaagaagtc aacaaatctt gtaaaaaata agtgtgttaa tttttga         777


<210>  81
<211>  258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD Y449A/F486A/N487A/Y489A tag

<400>  81

Ala Asp Pro Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Ser 
            20                  25                  30          


Arg Gly Gly Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn 
        35                  40                  45              


Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
    50                  55                  60                  


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
65                  70                  75                  80  


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
                85                  90                  95      


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
            100                 105                 110         


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
        115                 120                 125             


Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
    130                 135                 140                 


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
145                 150                 155                 160 


Lys Val Gly Gly Asn Ala Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
                165                 170                 175     


Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala 
            180                 185                 190         


Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro 
        195                 200                 205             


Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro 
    210                 215                 220                 


Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr 
225                 230                 235                 240 


Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val 
                245                 250                 255     


Asn Phe 
        


<210>  82
<211>  777
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD Y449A/F486A/N487A/Y489A tag

<400>  82
gcggatccct ggtctcatcc gcagtttgaa aagggaggtg gaagtggcgg tggttcgggc       60

ggatcggctt ggtcgcatcc ccaattcgaa aaatcaagag gcggcagggt ccagccaaca      120

gagtctattg tccgcttccc aaacatcaca aatttgtgtc ctttcggtga ggttttcaat      180

gctacaaggt ttgcatctgt gtacgcctgg aaccgcaagc gtatctccaa ctgcgtggca      240

gactactctg tactttataa ttctgcttct ttttcaacct ttaaatgcta tggagtatcg      300

cctactaaac tgaacgattt gtgttttacc aacgtgtatg cagactcatt cgtgataagg      360

ggcgatgagg tacgccaaat cgctcccgga cagaccggca agattgcgga ttacaactac      420

aagctcccag atgacttcac gggctgcgta atcgcatgga attctaataa ccttgatagt      480

aaggtcggtg gtaatgcgaa ttatctgtat cgtctgtttc gtaaatccaa cctgaaaccg      540

tttgaaagag atatttcgac cgagatatat caagcgggct caaccccatg caacggtgtt      600

gaaggcgcgg cgtgtgcgtt tccgctccag agttacggtt ttcagcccac gaatggagta      660

ggataccaac cctaccgtgt tgtggtgctg agttttgaac tgctgcacgc accggcaact      720

gtgtgcggtc ccaagaagtc aacaaatctt gtaaaaaata agtgtgttaa tttttga         777


<210>  83
<211>  258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD Q498A/T500A/Y505A tag

<400>  83

Ala Asp Pro Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Ser 
            20                  25                  30          


Arg Gly Gly Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn 
        35                  40                  45              


Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
    50                  55                  60                  


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
65                  70                  75                  80  


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
                85                  90                  95      


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
            100                 105                 110         


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
        115                 120                 125             


Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
    130                 135                 140                 


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
145                 150                 155                 160 


Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
                165                 170                 175     


Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala 
            180                 185                 190         


Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro 
        195                 200                 205             


Leu Gln Ser Tyr Gly Phe Ala Pro Ala Asn Gly Val Gly Ala Gln Pro 
    210                 215                 220                 


Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr 
225                 230                 235                 240 


Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val 
                245                 250                 255     


Asn Phe 
        


<210>  84
<211>  777
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD Q498A/T500A/Y505A tag

<400>  84
gcggatccct ggtctcatcc gcagtttgaa aagggaggtg gaagtggcgg tggttcgggc       60

ggatcggctt ggtcgcatcc ccaattcgaa aaatcaagag gcggcagggt ccagccaaca      120

gagtctattg tccgcttccc aaacatcaca aatttgtgtc ctttcggtga ggttttcaat      180

gctacaaggt ttgcatctgt gtacgcctgg aaccgcaagc gtatctccaa ctgcgtggca      240

gactactctg tactttataa ttctgcttct ttttcaacct ttaaatgcta tggagtatcg      300

cctactaaac tgaacgattt gtgttttacc aacgtgtatg cagactcatt cgtgataagg      360

ggcgatgagg tacgccaaat cgctcccgga cagaccggca agattgcgga ttacaactac      420

aagctcccag atgacttcac gggctgcgta atcgcatgga attctaataa ccttgatagt      480

aaggtcggtg gtaattataa ttatctgtat cgtctgtttc gtaaatccaa cctgaaaccg      540

tttgaaagag atatttcgac cgagatatat caagcgggct caaccccatg caacggtgtt      600

gaaggcttca attgttactt tccgctccag agttacggtt ttgcgcccgc gaatggagta      660

ggagcgcaac cctaccgtgt tgtggtgctg agttttgaac tgctgcacgc accggcaact      720

gtgtgcggtc ccaagaagtc aacaaatctt gtaaaaaata agtgtgttaa tttttga         777


<210>  85
<211>  258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD Q493A/Q498A/T500A/Y505A tag

<400>  85

Ala Asp Pro Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Ser 
            20                  25                  30          


Arg Gly Gly Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn 
        35                  40                  45              


Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
    50                  55                  60                  


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
65                  70                  75                  80  


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
                85                  90                  95      


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
            100                 105                 110         


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
        115                 120                 125             


Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
    130                 135                 140                 


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
145                 150                 155                 160 


Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
                165                 170                 175     


Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala 
            180                 185                 190         


Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro 
        195                 200                 205             


Leu Ala Ser Tyr Gly Phe Ala Pro Ala Asn Gly Val Gly Ala Gln Pro 
    210                 215                 220                 


Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr 
225                 230                 235                 240 


Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val 
                245                 250                 255     


Asn Phe 
        


<210>  86
<211>  777
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD Q493A/Q498A/T500A/Y505A tag

<400>  86
gcggatccct ggtctcatcc gcagtttgaa aagggaggtg gaagtggcgg tggttcgggc       60

ggatcggctt ggtcgcatcc ccaattcgaa aaatcaagag gcggcagggt ccagccaaca      120

gagtctattg tccgcttccc aaacatcaca aatttgtgtc ctttcggtga ggttttcaat      180

gctacaaggt ttgcatctgt gtacgcctgg aaccgcaagc gtatctccaa ctgcgtggca      240

gactactctg tactttataa ttctgcttct ttttcaacct ttaaatgcta tggagtatcg      300

cctactaaac tgaacgattt gtgttttacc aacgtgtatg cagactcatt cgtgataagg      360

ggcgatgagg tacgccaaat cgctcccgga cagaccggca agattgcgga ttacaactac      420

aagctcccag atgacttcac gggctgcgta atcgcatgga attctaataa ccttgatagt      480

aaggtcggtg gtaattataa ttatctgtat cgtctgtttc gtaaatccaa cctgaaaccg      540

tttgaaagag atatttcgac cgagatatat caagcgggct caaccccatg caacggtgtt      600

gaaggcttca attgttactt tccgctcgcg agttacggtt ttgcgcccgc gaatggagta      660

ggagcgcaac cctaccgtgt tgtggtgctg agttttgaac tgctgcacgc accggcaact      720

gtgtgcggtc ccaagaagtc aacaaatctt gtaaaaaata agtgtgttaa tttttga         777


<210>  87
<211>  258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD Y453A/L455A/F456A/Q493A tag

<400>  87

Ala Asp Pro Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Ser 
            20                  25                  30          


Arg Gly Gly Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn 
        35                  40                  45              


Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
    50                  55                  60                  


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
65                  70                  75                  80  


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
                85                  90                  95      


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
            100                 105                 110         


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
        115                 120                 125             


Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
    130                 135                 140                 


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
145                 150                 155                 160 


Lys Val Gly Gly Asn Tyr Asn Tyr Leu Ala Arg Ala Ala Arg Lys Ser 
                165                 170                 175     


Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala 
            180                 185                 190         


Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro 
        195                 200                 205             


Leu Ala Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro 
    210                 215                 220                 


Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr 
225                 230                 235                 240 


Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val 
                245                 250                 255     


Asn Phe 
        


<210>  88
<211>  777
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD Y453A/L455A/F456A/Q493A tag

<400>  88
gcggatccct ggtctcatcc gcagtttgaa aagggaggtg gaagtggcgg tggttcgggc       60

ggatcggctt ggtcgcatcc ccaattcgaa aaatcaagag gcggcagggt ccagccaaca      120

gagtctattg tccgcttccc aaacatcaca aatttgtgtc ctttcggtga ggttttcaat      180

gctacaaggt ttgcatctgt gtacgcctgg aaccgcaagc gtatctccaa ctgcgtggca      240

gactactctg tactttataa ttctgcttct ttttcaacct ttaaatgcta tggagtatcg      300

cctactaaac tgaacgattt gtgttttacc aacgtgtatg cagactcatt cgtgataagg      360

ggcgatgagg tacgccaaat cgctcccgga cagaccggca agattgcgga ttacaactac      420

aagctcccag atgacttcac gggctgcgta atcgcatgga attctaataa ccttgatagt      480

aaggtcggtg gtaattataa ttatctggcg cgtgcggcgc gtaaatccaa cctgaaaccg      540

tttgaaagag atatttcgac cgagatatat caagcgggct caaccccatg caacggtgtt      600

gaaggcttca attgttactt tccgctcgcg agttacggtt ttcagcccac gaatggagta      660

ggataccaac cctaccgtgt tgtggtgctg agttttgaac tgctgcacgc accggcaact      720

gtgtgcggtc ccaagaagtc aacaaatctt gtaaaaaata agtgtgttaa tttttga         777


<210>  89
<211>  258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD F486A/N487A/Y489A/Q498A/T500A/Y505A tag

<400>  89

Ala Asp Pro Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Ser 
            20                  25                  30          


Arg Gly Gly Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn 
        35                  40                  45              


Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
    50                  55                  60                  


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
65                  70                  75                  80  


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
                85                  90                  95      


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
            100                 105                 110         


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
        115                 120                 125             


Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
    130                 135                 140                 


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
145                 150                 155                 160 


Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
                165                 170                 175     


Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala 
            180                 185                 190         


Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro 
        195                 200                 205             


Leu Gln Ser Tyr Gly Phe Ala Pro Ala Asn Gly Val Gly Ala Gln Pro 
    210                 215                 220                 


Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr 
225                 230                 235                 240 


Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val 
                245                 250                 255     


Asn Phe 
        


<210>  90
<211>  777
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD F486A/N487A/Y489A/Q498A/T500A/Y505A tag

<400>  90
gcggatccct ggtctcatcc gcagtttgaa aagggaggtg gaagtggcgg tggttcgggc       60

ggatcggctt ggtcgcatcc ccaattcgaa aaatcaagag gcggcagggt ccagccaaca      120

gagtctattg tccgcttccc aaacatcaca aatttgtgtc ctttcggtga ggttttcaat      180

gctacaaggt ttgcatctgt gtacgcctgg aaccgcaagc gtatctccaa ctgcgtggca      240

gactactctg tactttataa ttctgcttct ttttcaacct ttaaatgcta tggagtatcg      300

cctactaaac tgaacgattt gtgttttacc aacgtgtatg cagactcatt cgtgataagg      360

ggcgatgagg tacgccaaat cgctcccgga cagaccggca agattgcgga ttacaactac      420

aagctcccag atgacttcac gggctgcgta atcgcatgga attctaataa ccttgatagt      480

aaggtcggtg gtaattataa ttatctgtat cgtctgtttc gtaaatccaa cctgaaaccg      540

tttgaaagag atatttcgac cgagatatat caagcgggct caaccccatg caacggtgtt      600

gaaggcgcgg cgtgtgcgtt tccgctccag agttacggtt ttgcgcccgc gaatggagta      660

ggagcgcaac cctaccgtgt tgtggtgctg agttttgaac tgctgcacgc accggcaact      720

gtgtgcggtc ccaagaagtc aacaaatctt gtaaaaaata agtgtgttaa tttttga         777


<210>  91
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD F486A tag

<400>  91

Ala Asp Pro Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Ser 
            20                  25                  30          


Arg Gly Gly Leu Glu Val Leu Phe Gln Gly Pro Arg Val Gln Pro Thr 
        35                  40                  45              


Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
    50                  55                  60                  


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
65                  70                  75                  80  


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
                85                  90                  95      


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
            100                 105                 110         


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
        115                 120                 125             


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala 
    130                 135                 140                 


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
145                 150                 155                 160 


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
                165                 170                 175     


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
            180                 185                 190         


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
        195                 200                 205             


Glu Gly Ala Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
    210                 215                 220                 


Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
225                 230                 235                 240 


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
                245                 250                 255     


Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
            260                 265     


<210>  92
<211>  801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD F486A tag

<400>  92
gcggatccct ggtctcatcc gcagtttgaa aagggaggtg gaagtggcgg tggttcgggc       60

ggatcggctt ggtcgcatcc ccaattcgaa aaatcaagag gcggcctgga ggttctgttt      120

caaggtccaa gggtccagcc aacagagtct attgtccgct tcccaaacat cacaaatttg      180

tgtcctttcg gtgaggtttt caatgctaca aggtttgcat ctgtgtacgc ctggaaccgc      240

aagcgtatct ccaactgcgt ggcagactac tctgtacttt ataattctgc ttctttttca      300

acctttaaat gctatggagt atcgcctact aaactgaacg atttgtgttt taccaacgtg      360

tatgcagact cattcgtgat aaggggcgat gaggtacgcc aaatcgctcc cggacagacc      420

ggcaagattg cggattacaa ctacaagctc ccagatgact tcacgggctg cgtaatcgca      480

tggaattcta ataaccttga tagtaaggtc ggtggtaatt ataattatct gtatcgtctg      540

tttcgtaaat ccaacctgaa accgtttgaa agagatattt cgaccgagat atatcaagcg      600

ggctcaaccc catgcaacgg tgttgaaggc gcgaattgtt actttccgct ccagagttac      660

ggttttcagc ccacgaatgg agtaggatac caaccctacc gtgttgtggt gctgagtttt      720

gaactgctgc acgcaccggc aactgtgtgc ggtcccaaga agtcaacaaa tcttgtaaaa      780

aataagtgtg ttaatttttg a                                                801


<210>  93
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD N487A tag

<400>  93

Ala Asp Pro Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Ser 
            20                  25                  30          


Arg Gly Gly Leu Glu Val Leu Phe Gln Gly Pro Arg Val Gln Pro Thr 
        35                  40                  45              


Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
    50                  55                  60                  


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
65                  70                  75                  80  


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
                85                  90                  95      


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
            100                 105                 110         


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
        115                 120                 125             


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala 
    130                 135                 140                 


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
145                 150                 155                 160 


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
                165                 170                 175     


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
            180                 185                 190         


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
        195                 200                 205             


Glu Gly Phe Ala Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
    210                 215                 220                 


Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
225                 230                 235                 240 


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
                245                 250                 255     


Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
            260                 265     


<210>  94
<211>  800
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD N487A tag

<400>  94
gcggatccct ggtctcatcc gcagtttgaa aagggaggtg gaagtggcgg tggttcgggc       60

ggatcggctt ggtcgcatcc ccaattcgaa aaatcaagag gcggcctgga ggttctgttt      120

caaggtccaa gggtccagcc aacagagtct attgtccgct tcccaaacat cacaaatttg      180

tgtcctttcg gtgaggtttt caatgctaca aggtttgcat ctgtgtacgc ctggaaccgc      240

aagcgtatct ccaactgcgt ggcagactac tctgtacttt ataattctgc ttctttttca      300

acctttaaat gctatggagt atcgcctact aaactgaacg atttgtgttt taccaacgtg      360

tatgcagact cattcgtgat aaggggcgat gaggtacgcc aaatcgctcc cggacagacc      420

ggcaagattg cggattacaa ctacaagctc ccagatgact tcacgggctg cgtaatcgca      480

tggaattcta ataaccttga tagtaaggtc ggtggtaatt ataattatct gtatcgtctg      540

tttcgtaaat ccaacctgaa accgtttgaa agagatattt cgaccgagat atatcaagcg      600

ggctcaaccc catgcaacgg tgttgaaggc ttcgcgtgtt actttccgct ccagagttac      660

ggttttcagc ccacgaatgg agtaggatac caaccctacc gtgttgtggt gctgagtttt      720

gaactgctgc acgcaccggc aactgtgtgc ggtcccaaga agtcaacaaa tcttgtaaaa      780

aataagtgtg ttaatttttg                                                  800


<210>  95
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wild type RBD 3C tag

<400>  95

Ala Asp Pro Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Ser 
            20                  25                  30          


Arg Gly Gly Leu Glu Val Leu Phe Gln Gly Pro Arg Val Gln Pro Thr 
        35                  40                  45              


Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
    50                  55                  60                  


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
65                  70                  75                  80  


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
                85                  90                  95      


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
            100                 105                 110         


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
        115                 120                 125             


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala 
    130                 135                 140                 


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
145                 150                 155                 160 


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
                165                 170                 175     


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
            180                 185                 190         


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
        195                 200                 205             


Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
    210                 215                 220                 


Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
225                 230                 235                 240 


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
                245                 250                 255     


Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
            260                 265     


<210>  96
<211>  801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wild type RBD 3C tag

<400>  96
gcggatccct ggtctcatcc gcagtttgaa aagggaggtg gaagtggcgg tggttcgggc       60

ggatcggctt ggtcgcatcc ccaattcgaa aaatcaagag gcggcctgga ggttctgttt      120

caaggtccaa gggtccagcc aacagagtct attgtccgct tcccaaacat cacaaatttg      180

tgtcctttcg gtgaggtttt caatgctaca aggtttgcat ctgtgtacgc ctggaaccgc      240

aagcgtatct ccaactgcgt ggcagactac tctgtacttt ataattctgc ttctttttca      300

acctttaaat gctatggagt atcgcctact aaactgaacg atttgtgttt taccaacgtg      360

tatgcagact cattcgtgat aaggggcgat gaggtacgcc aaatcgctcc cggacagacc      420

ggcaagattg cggattacaa ctacaagctc ccagatgact tcacgggctg cgtaatcgca      480

tggaattcta ataaccttga tagtaaggtc ggtggtaatt ataattatct gtatcgtctg      540

tttcgtaaat ccaacctgaa accgtttgaa agagatattt cgaccgagat atatcaagcg      600

ggctcaaccc catgcaacgg tgttgaaggc ttcaattgtt actttccgct ccagagttac      660

ggttttcagc ccacgaatgg agtaggatac caaccctacc gtgttgtggt gctgagtttt      720

gaactgctgc acgcaccggc aactgtgtgc ggtcccaaga agtcaacaaa tcttgtaaaa      780

aataagtgtg ttaatttttg a                                                801


<210>  97
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S with wild type RBD

<400>  97

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  98
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S with wild type RBD DNA

<400>  98
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcttcaa ctgctacttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  99
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S with RBD A475W

<400>  99

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Trp Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  100
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S with RBD A475W

<400>  100
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc agtggggcag caccccttgt     1440

aacggcgtgg aaggcttcaa ctgctacttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  101
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S with RBD N487W

<400>  101

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Trp Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  102
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S with RBD N487W

<400>  102
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcttctg gtgctacttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  103
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S with RBD Y489A

<400>  103

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  104
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S with RBD Y489A

<400>  104
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcttcaa ctgcgcgttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  105
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S with N-RBD (F486A/N487A/Y489A)

<400>  105

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  106
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S with N-RBD (F486A/N487A/Y489A)

<400>  106
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcgcggc gtgcgcgttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  107
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S with N-RBD + Y449A

<400>  107

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Ala Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  108
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S with N-RBD + Y449A

<400>  108
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caacgcgaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcgcggc gtgcgcgttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  109
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S with C-RBD (Q498A/T500A/Y505A)

<400>  109

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Ala Pro Ala Asn Gly Val Gly Ala Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  110
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S with C-RBD (Q498A/T500A/Y505A)

<400>  110
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcttcaa ctgctacttc ccactgcagt cctacggctt tgcgcccgcg     1500

aatggcgtgg gcgcgcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  111
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S with C-RBD + Q493A

<400>  111

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser Tyr Gly 
                485                 490                 495     


Phe Ala Pro Ala Asn Gly Val Gly Ala Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  112
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S with C-RBD + Q493A

<400>  112
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcttcaa ctgctacttc ccactggcgt cctacggctt tgcgcccgcg     1500

aatggcgtgg gcgcgcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  113
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S with M-RBD (Y453A/L455A/F456A/Q493A)

<400>  113

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Ala Arg Ala Ala Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  114
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S with M-RBD (Y453A/L455A/F456A/Q493A)

<400>  114
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctggcgc gggcggcgcg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcttcaa ctgctacttc ccactggcgt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  115
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S with RBD N+C ( F486A/N487A/Y489A/Q498A/T500A/Y505A)

<400>  115

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Ala Pro Ala Asn Gly Val Gly Ala Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  116
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S with RBD N+C ( F486A/N487A/Y489A/Q498A/T500A/Y505A)

<400>  116
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcgcggc gtgcgcgttc ccactgcagt cctacggctt tgcgcccgcg     1500

aatggcgtgg gcgcgcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  117
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S with RBD F486A

<400>  117

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Ala Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  118
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S with RBD F486A

<400>  118
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcgcgaa ctgctacttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  119
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S with RBD N487A

<400>  119

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Ala Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  120
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S with RBD N487A

<400>  120
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcttcgc gtgctacttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  121
<211>  1269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant B.1.1.7 (20B/501Y.v1)

<400>  121

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp Asn Pro 
65                  70                  75                  80  


Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu Lys Ser 
                85                  90                  95      


Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser Lys Thr 
            100                 105                 110         


Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile Lys Val 
        115                 120                 125             


Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val His Lys Asn 
    130                 135                 140                 


Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr Ser Ser Ala Asn 
145                 150                 155                 160 


Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp Leu Glu 
                165                 170                 175     


Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe Lys Asn 
            180                 185                 190         


Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile Asn Leu 
        195                 200                 205             


Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro Leu Val Asp 
    210                 215                 220                 


Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu Ala Leu 
225                 230                 235                 240 


His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr Ala 
                245                 250                 255     


Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe Leu 
            260                 265                 270         


Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys Ala 
        275                 280                 285             


Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr Val 
    290                 295                 300                 


Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr Glu 
305                 310                 315                 320 


Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu 
                325                 330                 335     


Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys 
            340                 345                 350         


Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala 
        355                 360                 365             


Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn 
    370                 375                 380                 


Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly 
385                 390                 395                 400 


Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp 
                405                 410                 415     


Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp 
            420                 425                 430         


Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu 
        435                 440                 445             


Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile 
    450                 455                 460                 


Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu 
465                 470                 475                 480 


Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr 
                485                 490                 495     


Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu 
            500                 505                 510         


Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn 
        515                 520                 525             


Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr Gly 
    530                 535                 540                 


Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln Gln 
545                 550                 555                 560 


Phe Gly Arg Asp Ile Asp Asp Thr Thr Asp Ala Val Arg Asp Pro Gln 
                565                 570                 575     


Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val Ser 
            580                 585                 590         


Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu Tyr 
        595                 600                 605             


Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp Gln 
    610                 615                 620                 


Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe Gln 
625                 630                 635                 640 


Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser Tyr 
                645                 650                 655     


Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln Thr 
            660                 665                 670         


Gln Thr Asn Ser His Arg Arg Ala Arg Ser Val Ala Ser Gln Ser Ile 
        675                 680                 685             


Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala Tyr Ser 
    690                 695                 700                 


Asn Asn Ser Ile Ala Ile Pro Ile Asn Phe Thr Ile Ser Val Thr Thr 
705                 710                 715                 720 


Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr Met 
                725                 730                 735     


Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln Tyr 
            740                 745                 750         


Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala Val 
        755                 760                 765             


Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln Ile 
    770                 775                 780                 


Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser Gln 
785                 790                 795                 800 


Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp 
                805                 810                 815     


Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln 
            820                 825                 830         


Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala 
        835                 840                 845             


Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu 
    850                 855                 860                 


Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser 
865                 870                 875                 880 


Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met 
                885                 890                 895     


Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu 
            900                 905                 910         


Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly 
        915                 920                 925             


Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu 
    930                 935                 940                 


Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys 
945                 950                 955                 960 


Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile 
                965                 970                 975     


Leu Ala Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp Arg Leu 
            980                 985                 990         


Ile Thr Gly Arg Leu Gln Ser Leu  Gln Thr Tyr Val Thr  Gln Gln Leu 
        995                 1000                 1005             


Ile Arg  Ala Ala Glu Ile Arg  Ala Ser Ala Asn Leu  Ala Ala Thr 
    1010                 1015                 1020             


Lys Met  Ser Glu Cys Val Leu  Gly Gln Ser Lys Arg  Val Asp Phe 
    1025                 1030                 1035             


Cys Gly  Lys Gly Tyr His Leu  Met Ser Phe Pro Gln  Ser Ala Pro 
    1040                 1045                 1050             


His Gly  Val Val Phe Leu His  Val Thr Tyr Val Pro  Ala Gln Glu 
    1055                 1060                 1065             


Lys Asn  Phe Thr Thr Ala Pro  Ala Ile Cys His Asp  Gly Lys Ala 
    1070                 1075                 1080             


His Phe  Pro Arg Glu Gly Val  Phe Val Ser Asn Gly  Thr His Trp 
    1085                 1090                 1095             


Phe Val  Thr Gln Arg Asn Phe  Tyr Glu Pro Gln Ile  Ile Thr Thr 
    1100                 1105                 1110             


His Asn  Thr Phe Val Ser Gly  Asn Cys Asp Val Val  Ile Gly Ile 
    1115                 1120                 1125             


Val Asn  Asn Thr Val Tyr Asp  Pro Leu Gln Pro Glu  Leu Asp Ser 
    1130                 1135                 1140             


Phe Lys  Glu Glu Leu Asp Lys  Tyr Phe Lys Asn His  Thr Ser Pro 
    1145                 1150                 1155             


Asp Val  Asp Leu Gly Asp Ile  Ser Gly Ile Asn Ala  Ser Val Val 
    1160                 1165                 1170             


Asn Ile  Gln Lys Glu Ile Asp  Arg Leu Asn Glu Val  Ala Lys Asn 
    1175                 1180                 1185             


Leu Asn  Glu Ser Leu Ile Asp  Leu Gln Glu Leu Gly  Lys Tyr Glu 
    1190                 1195                 1200             


Gln Tyr  Ile Lys Trp Pro Trp  Tyr Ile Trp Leu Gly  Phe Ile Ala 
    1205                 1210                 1215             


Gly Leu  Ile Ala Ile Val Met  Val Thr Ile Met Leu  Cys Cys Met 
    1220                 1225                 1230             


Thr Ser  Cys Cys Ser Cys Leu  Lys Gly Cys Cys Ser  Cys Gly Ser 
    1235                 1240                 1245             


Cys Cys  Lys Phe Asp Glu Asp  Asp Ser Glu Pro Val  Leu Lys Gly 
    1250                 1255                 1260             


Val Lys  Leu His Tyr Thr 
    1265                 


<210>  122
<211>  3810
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of  variant B.1.1.7 (20B/501Y.v1)

<400>  122
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catctccggc accaatggca ccaagagatt cgacaacccc      240

gtgctgccct tcaacgacgg ggtgtacttt gccagcaccg agaagtccaa catcatcaga      300

ggctggatct tcggcaccac actggacagc aagacccaga gcctgctgat cgtgaacaac      360

gccaccaacg tggtcatcaa agtgtgcgag ttccagttct gcaacgaccc cttcctgggc      420

gtccacaaga acaacaagag ctggatggaa agcgagttcc gggtgtacag cagcgccaac      480

aactgcacct tcgagtacgt gtcccagcct ttcctgatgg acctggaagg caagcagggc      540

aacttcaaga acctgcgcga gttcgtgttt aagaacatcg acggctactt caagatctac      600

agcaagcaca cccctatcaa cctcgtgcgg gatctgcctc agggcttctc tgctctggaa      660

cccctggtgg atctgcccat cggcatcaac atcacccggt ttcagacact gctggccctg      720

cacagaagct acctgacacc tggcgatagc agcagcggat ggacagctgg tgccgccgct      780

tactatgtgg gctacctgca gcctagaacc ttcctgctga agtacaacga gaacggcacc      840

atcaccgacg ccgtggattg tgctctggat cctctgagcg agacaaagtg caccctgaag      900

tccttcaccg tggaaaaggg catctaccag accagcaact tccgggtgca gcccaccgaa      960

tccatcgtgc ggttccccaa tatcaccaat ctgtgcccct tcggcgaggt gttcaatgcc     1020

accagattcg cctctgtgta cgcctggaac cggaagcgga tcagcaattg cgtggccgac     1080

tactccgtgc tgtacaactc cgccagcttc agcaccttca agtgctacgg cgtgtcccct     1140

accaagctga acgacctgtg cttcacaaac gtgtacgccg acagcttcgt gatccgggga     1200

gatgaagtgc ggcagattgc ccctggacag acaggcaaga tcgccgacta caactacaag     1260

ctgcccgacg acttcaccgg ctgtgtgatt gcctggaaca gcaacaacct ggactccaaa     1320

gtcggcggca actacaatta cctgtaccgg ctgttccgga agtccaatct gaagcccttc     1380

gagcgggaca tctccaccga gatctatcag gccggcagca ccccttgtaa cggcgtggaa     1440

ggcttcaact gctacttccc actgcagtcc tacggctttc agcccacata cggcgtgggc     1500

tatcagccct acagagtggt ggtgctgagc ttcgaactgc tgcatgcccc tgccacagtg     1560

tgcggcccta agaaaagcac caatctcgtg aagaacaaat gcgtgaactt caacttcaac     1620

ggcctgaccg gcaccggcgt gctgacagag agcaacaaga agttcctgcc attccagcag     1680

tttggccggg atatcgatga taccacagac gccgttagag atccccagac actggaaatc     1740

ctggacatca ccccttgcag cttcggcgga gtgtctgtga tcacccctgg caccaacacc     1800

agcaatcagg tggcagtgct gtaccaggac gtgaactgta ccgaagtgcc cgtggccatt     1860

cacgccgatc agctgacacc tacatggcgg gtgtactcca ccggcagcaa tgtgtttcag     1920

accagagccg gctgtctgat cggagccgag cacgtgaaca atagctacga gtgcgacatc     1980

cccatcggcg ctggaatctg cgccagctac cagacacaga caaacagcca ccggagagcc     2040

agaagcgtgg ccagccagag catcattgcc tacacaatgt ctctgggcgc cgagaacagc     2100

gtggcctact ccaacaactc tatcgctatc cccatcaact tcaccatcag cgtgaccaca     2160

gagatcctgc ctgtgtccat gaccaagacc agcgtggact gcaccatgta catctgcggc     2220

gattccaccg agtgctccaa cctgctgctg cagtacggca gcttctgcac ccagctgaat     2280

agagccctga cagggatcgc cgtggaacag gacaagaaca cccaagaggt gttcgcccaa     2340

gtgaagcaga tctacaagac ccctcctatc aaggacttcg gcggcttcaa tttcagccag     2400

attctgcccg atcctagcaa gcccagcaag cggagcttca tcgaggacct gctgttcaac     2460

aaagtgacac tggccgacgc cggcttcatc aagcagtatg gcgattgtct gggcgacatt     2520

gccgccaggg atctgatttg cgcccagaag tttaacggac tgacagtgct gcctcctctg     2580

ctgaccgatg agatgatcgc ccagtacaca tctgccctgc tggccggcac aatcacaagc     2640

ggctggacat ttggagcagg cgccgctctg cagatcccct ttgctatgca gatggcctac     2700

cggttcaacg gcatcggagt gacccagaat gtgctgtacg agaaccagaa gctgatcgcc     2760

aaccagttca acagcgccat cggcaagatc caggacagcc tgagcagcac agcaagcgcc     2820

ctgggaaagc tgcaggacgt ggtcaaccag aatgcccagg cactgaacac cctggtcaag     2880

cagctgtcct ccaacttcgg cgccatcagc tctgtgctga acgatatcct ggccagactg     2940

gaccctcctg aggccgaggt gcagatcgac agactgatca caggcagact gcagagcctc     3000

cagacatacg tgacccagca gctgatcaga gccgccgaga ttagagcctc tgccaatctg     3060

gccgccacca agatgtctga gtgtgtgctg ggccagagca agagagtgga cttttgcggc     3120

aagggctacc acctgatgag cttccctcag tctgcccctc acggcgtggt gtttctgcac     3180

gtgacatatg tgcccgctca agagaagaat ttcaccaccg ctccagccat ctgccacgac     3240

ggcaaagccc actttcctag agaaggcgtg ttcgtgtcca acggcaccca ttggttcgtg     3300

acacagcgga acttctacga gccccagatc atcaccaccc acaacacctt cgtgtctggc     3360

aactgcgacg tcgtgatcgg cattgtgaac aataccgtgt acgaccctct gcagcccgag     3420

ctggacagct tcaaagagga actggacaag tactttaaga accacacaag ccccgacgtg     3480

gacctgggcg atatcagcgg aatcaatgcc agcgtcgtga acatccagaa agagatcgac     3540

cggctgaacg aggtggccaa gaatctgaac gagagcctga tcgacctgca agaactgggg     3600

aagtacgagc agtacatcaa gtggccctgg tacatctggc tgggctttat cgccggactg     3660

attgccatcg tgatggtcac aatcatgctg tgttgcatga ccagctgctg tagctgcctg     3720

aagggctgtt gtagctgtgg cagctgctgc aagttcgacg aggacgattc tgagcccgtg     3780

ctgaagggcg tgaaactgca ctacacatga                                      3810


<210>  123
<211>  1269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant B.1.1.7 + E484K (20B/501Y.v1)

<400>  123

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp Asn Pro 
65                  70                  75                  80  


Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu Lys Ser 
                85                  90                  95      


Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser Lys Thr 
            100                 105                 110         


Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile Lys Val 
        115                 120                 125             


Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val His Lys Asn 
    130                 135                 140                 


Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr Ser Ser Ala Asn 
145                 150                 155                 160 


Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp Leu Glu 
                165                 170                 175     


Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe Lys Asn 
            180                 185                 190         


Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile Asn Leu 
        195                 200                 205             


Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro Leu Val Asp 
    210                 215                 220                 


Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu Ala Leu 
225                 230                 235                 240 


His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr Ala 
                245                 250                 255     


Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe Leu 
            260                 265                 270         


Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys Ala 
        275                 280                 285             


Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr Val 
    290                 295                 300                 


Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr Glu 
305                 310                 315                 320 


Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu 
                325                 330                 335     


Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys 
            340                 345                 350         


Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala 
        355                 360                 365             


Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn 
    370                 375                 380                 


Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly 
385                 390                 395                 400 


Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp 
                405                 410                 415     


Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp 
            420                 425                 430         


Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu 
        435                 440                 445             


Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile 
    450                 455                 460                 


Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Lys 
465                 470                 475                 480 


Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr 
                485                 490                 495     


Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu 
            500                 505                 510         


Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn 
        515                 520                 525             


Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr Gly 
    530                 535                 540                 


Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln Gln 
545                 550                 555                 560 


Phe Gly Arg Asp Ile Asp Asp Thr Thr Asp Ala Val Arg Asp Pro Gln 
                565                 570                 575     


Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val Ser 
            580                 585                 590         


Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu Tyr 
        595                 600                 605             


Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp Gln 
    610                 615                 620                 


Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe Gln 
625                 630                 635                 640 


Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser Tyr 
                645                 650                 655     


Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln Thr 
            660                 665                 670         


Gln Thr Asn Ser His Arg Arg Ala Arg Ser Val Ala Ser Gln Ser Ile 
        675                 680                 685             


Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala Tyr Ser 
    690                 695                 700                 


Asn Asn Ser Ile Ala Ile Pro Ile Asn Phe Thr Ile Ser Val Thr Thr 
705                 710                 715                 720 


Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr Met 
                725                 730                 735     


Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln Tyr 
            740                 745                 750         


Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala Val 
        755                 760                 765             


Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln Ile 
    770                 775                 780                 


Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser Gln 
785                 790                 795                 800 


Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp 
                805                 810                 815     


Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln 
            820                 825                 830         


Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala 
        835                 840                 845             


Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu 
    850                 855                 860                 


Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser 
865                 870                 875                 880 


Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met 
                885                 890                 895     


Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu 
            900                 905                 910         


Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly 
        915                 920                 925             


Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu 
    930                 935                 940                 


Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys 
945                 950                 955                 960 


Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile 
                965                 970                 975     


Leu Ala Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp Arg Leu 
            980                 985                 990         


Ile Thr Gly Arg Leu Gln Ser Leu  Gln Thr Tyr Val Thr  Gln Gln Leu 
        995                 1000                 1005             


Ile Arg  Ala Ala Glu Ile Arg  Ala Ser Ala Asn Leu  Ala Ala Thr 
    1010                 1015                 1020             


Lys Met  Ser Glu Cys Val Leu  Gly Gln Ser Lys Arg  Val Asp Phe 
    1025                 1030                 1035             


Cys Gly  Lys Gly Tyr His Leu  Met Ser Phe Pro Gln  Ser Ala Pro 
    1040                 1045                 1050             


His Gly  Val Val Phe Leu His  Val Thr Tyr Val Pro  Ala Gln Glu 
    1055                 1060                 1065             


Lys Asn  Phe Thr Thr Ala Pro  Ala Ile Cys His Asp  Gly Lys Ala 
    1070                 1075                 1080             


His Phe  Pro Arg Glu Gly Val  Phe Val Ser Asn Gly  Thr His Trp 
    1085                 1090                 1095             


Phe Val  Thr Gln Arg Asn Phe  Tyr Glu Pro Gln Ile  Ile Thr Thr 
    1100                 1105                 1110             


His Asn  Thr Phe Val Ser Gly  Asn Cys Asp Val Val  Ile Gly Ile 
    1115                 1120                 1125             


Val Asn  Asn Thr Val Tyr Asp  Pro Leu Gln Pro Glu  Leu Asp Ser 
    1130                 1135                 1140             


Phe Lys  Glu Glu Leu Asp Lys  Tyr Phe Lys Asn His  Thr Ser Pro 
    1145                 1150                 1155             


Asp Val  Asp Leu Gly Asp Ile  Ser Gly Ile Asn Ala  Ser Val Val 
    1160                 1165                 1170             


Asn Ile  Gln Lys Glu Ile Asp  Arg Leu Asn Glu Val  Ala Lys Asn 
    1175                 1180                 1185             


Leu Asn  Glu Ser Leu Ile Asp  Leu Gln Glu Leu Gly  Lys Tyr Glu 
    1190                 1195                 1200             


Gln Tyr  Ile Lys Trp Pro Trp  Tyr Ile Trp Leu Gly  Phe Ile Ala 
    1205                 1210                 1215             


Gly Leu  Ile Ala Ile Val Met  Val Thr Ile Met Leu  Cys Cys Met 
    1220                 1225                 1230             


Thr Ser  Cys Cys Ser Cys Leu  Lys Gly Cys Cys Ser  Cys Gly Ser 
    1235                 1240                 1245             


Cys Cys  Lys Phe Asp Glu Asp  Asp Ser Glu Pro Val  Leu Lys Gly 
    1250                 1255                 1260             


Val Lys  Leu His Tyr Thr 
    1265                 


<210>  124
<211>  3810
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant B.1.1.7 + E484K (20B/501Y.v1)

<400>  124
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catctccggc accaatggca ccaagagatt cgacaacccc      240

gtgctgccct tcaacgacgg ggtgtacttt gccagcaccg agaagtccaa catcatcaga      300

ggctggatct tcggcaccac actggacagc aagacccaga gcctgctgat cgtgaacaac      360

gccaccaacg tggtcatcaa agtgtgcgag ttccagttct gcaacgaccc cttcctgggc      420

gtccacaaga acaacaagag ctggatggaa agcgagttcc gggtgtacag cagcgccaac      480

aactgcacct tcgagtacgt gtcccagcct ttcctgatgg acctggaagg caagcagggc      540

aacttcaaga acctgcgcga gttcgtgttt aagaacatcg acggctactt caagatctac      600

agcaagcaca cccctatcaa cctcgtgcgg gatctgcctc agggcttctc tgctctggaa      660

cccctggtgg atctgcccat cggcatcaac atcacccggt ttcagacact gctggccctg      720

cacagaagct acctgacacc tggcgatagc agcagcggat ggacagctgg tgccgccgct      780

tactatgtgg gctacctgca gcctagaacc ttcctgctga agtacaacga gaacggcacc      840

atcaccgacg ccgtggattg tgctctggat cctctgagcg agacaaagtg caccctgaag      900

tccttcaccg tggaaaaggg catctaccag accagcaact tccgggtgca gcccaccgaa      960

tccatcgtgc ggttccccaa tatcaccaat ctgtgcccct tcggcgaggt gttcaatgcc     1020

accagattcg cctctgtgta cgcctggaac cggaagcgga tcagcaattg cgtggccgac     1080

tactccgtgc tgtacaactc cgccagcttc agcaccttca agtgctacgg cgtgtcccct     1140

accaagctga acgacctgtg cttcacaaac gtgtacgccg acagcttcgt gatccgggga     1200

gatgaagtgc ggcagattgc ccctggacag acaggcaaga tcgccgacta caactacaag     1260

ctgcccgacg acttcaccgg ctgtgtgatt gcctggaaca gcaacaacct ggactccaaa     1320

gtcggcggca actacaatta cctgtaccgg ctgttccgga agtccaatct gaagcccttc     1380

gagcgggaca tctccaccga gatctatcag gccggcagca ccccttgtaa cggcgtgaaa     1440

ggcttcaact gctacttccc actgcagtcc tacggctttc agcccacata cggcgtgggc     1500

tatcagccct acagagtggt ggtgctgagc ttcgaactgc tgcatgcccc tgccacagtg     1560

tgcggcccta agaaaagcac caatctcgtg aagaacaaat gcgtgaactt caacttcaac     1620

ggcctgaccg gcaccggcgt gctgacagag agcaacaaga agttcctgcc attccagcag     1680

tttggccggg atatcgatga taccacagac gccgttagag atccccagac actggaaatc     1740

ctggacatca ccccttgcag cttcggcgga gtgtctgtga tcacccctgg caccaacacc     1800

agcaatcagg tggcagtgct gtaccaggac gtgaactgta ccgaagtgcc cgtggccatt     1860

cacgccgatc agctgacacc tacatggcgg gtgtactcca ccggcagcaa tgtgtttcag     1920

accagagccg gctgtctgat cggagccgag cacgtgaaca atagctacga gtgcgacatc     1980

cccatcggcg ctggaatctg cgccagctac cagacacaga caaacagcca ccggagagcc     2040

agaagcgtgg ccagccagag catcattgcc tacacaatgt ctctgggcgc cgagaacagc     2100

gtggcctact ccaacaactc tatcgctatc cccatcaact tcaccatcag cgtgaccaca     2160

gagatcctgc ctgtgtccat gaccaagacc agcgtggact gcaccatgta catctgcggc     2220

gattccaccg agtgctccaa cctgctgctg cagtacggca gcttctgcac ccagctgaat     2280

agagccctga cagggatcgc cgtggaacag gacaagaaca cccaagaggt gttcgcccaa     2340

gtgaagcaga tctacaagac ccctcctatc aaggacttcg gcggcttcaa tttcagccag     2400

attctgcccg atcctagcaa gcccagcaag cggagcttca tcgaggacct gctgttcaac     2460

aaagtgacac tggccgacgc cggcttcatc aagcagtatg gcgattgtct gggcgacatt     2520

gccgccaggg atctgatttg cgcccagaag tttaacggac tgacagtgct gcctcctctg     2580

ctgaccgatg agatgatcgc ccagtacaca tctgccctgc tggccggcac aatcacaagc     2640

ggctggacat ttggagcagg cgccgctctg cagatcccct ttgctatgca gatggcctac     2700

cggttcaacg gcatcggagt gacccagaat gtgctgtacg agaaccagaa gctgatcgcc     2760

aaccagttca acagcgccat cggcaagatc caggacagcc tgagcagcac agcaagcgcc     2820

ctgggaaagc tgcaggacgt ggtcaaccag aatgcccagg cactgaacac cctggtcaag     2880

cagctgtcct ccaacttcgg cgccatcagc tctgtgctga acgatatcct ggccagactg     2940

gaccctcctg aggccgaggt gcagatcgac agactgatca caggcagact gcagagcctc     3000

cagacatacg tgacccagca gctgatcaga gccgccgaga ttagagcctc tgccaatctg     3060

gccgccacca agatgtctga gtgtgtgctg ggccagagca agagagtgga cttttgcggc     3120

aagggctacc acctgatgag cttccctcag tctgcccctc acggcgtggt gtttctgcac     3180

gtgacatatg tgcccgctca agagaagaat ttcaccaccg ctccagccat ctgccacgac     3240

ggcaaagccc actttcctag agaaggcgtg ttcgtgtcca acggcaccca ttggttcgtg     3300

acacagcgga acttctacga gccccagatc atcaccaccc acaacacctt cgtgtctggc     3360

aactgcgacg tcgtgatcgg cattgtgaac aataccgtgt acgaccctct gcagcccgag     3420

ctggacagct tcaaagagga actggacaag tactttaaga accacacaag ccccgacgtg     3480

gacctgggcg atatcagcgg aatcaatgcc agcgtcgtga acatccagaa agagatcgac     3540

cggctgaacg aggtggccaa gaatctgaac gagagcctga tcgacctgca agaactgggg     3600

aagtacgagc agtacatcaa gtggccctgg tacatctggc tgggctttat cgccggactg     3660

attgccatcg tgatggtcac aatcatgctg tgttgcatga ccagctgctg tagctgcctg     3720

aagggctgtt gtagctgtgg cagctgctgc aagttcgacg aggacgattc tgagcccgtg     3780

ctgaagggcg tgaaactgca ctacacatga                                      3810


<210>  125
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant P.1 (20J/501Y.V3)

<400>  125

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Phe Thr Asn Arg Thr Gln Leu Pro Ser Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Tyr Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Ser Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Thr Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu Tyr Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Ile Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Phe Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  126
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant P.1 (20J/501Y.V3)

<400>  126
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cttcaccaac       60

agaacacagc tgccttcagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa ctaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgagc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcac gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtga aaggcttcaa ctgctacttc ccactgcagt cctacggctt tcagcccaca     1500

tatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg gcgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agtacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccat caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcttcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  127
<211>  1271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant B.1.351 (20H/501Y.V2)

<400>  127

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Phe Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Ala 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Gly Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp 
                245                 250                 255     


Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr 
            260                 265                 270         


Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp 
        275                 280                 285             


Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe 
    290                 295                 300                 


Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro 
305                 310                 315                 320 


Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe 
                325                 330                 335     


Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn 
            340                 345                 350         


Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn 
        355                 360                 365             


Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys 
    370                 375                 380                 


Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile 
385                 390                 395                 400 


Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Asn Ile 
                405                 410                 415     


Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile 
            420                 425                 430         


Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn 
        435                 440                 445             


Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg 
    450                 455                 460                 


Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly 
465                 470                 475                 480 


Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln 
                485                 490                 495     


Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser 
            500                 505                 510         


Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser 
        515                 520                 525             


Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu 
    530                 535                 540                 


Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe 
545                 550                 555                 560 


Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp 
                565                 570                 575     


Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly 
            580                 585                 590         


Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val 
        595                 600                 605             


Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala 
    610                 615                 620                 


Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val 
625                 630                 635                 640 


Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn 
                645                 650                 655     


Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr 
            660                 665                 670         


Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala Ser Gln 
        675                 680                 685             


Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Val Glu Asn Ser Val Ala 
    690                 695                 700                 


Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val 
705                 710                 715                 720 


Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys 
                725                 730                 735     


Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu 
            740                 745                 750         


Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile 
        755                 760                 765             


Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys 
    770                 775                 780                 


Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe 
785                 790                 795                 800 


Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile 
                805                 810                 815     


Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile 
            820                 825                 830         


Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile 
        835                 840                 845             


Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr 
    850                 855                 860                 


Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile 
865                 870                 875                 880 


Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe 
                885                 890                 895     


Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn 
            900                 905                 910         


Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala 
        915                 920                 925             


Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly 
    930                 935                 940                 


Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu 
945                 950                 955                 960 


Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn 
                965                 970                 975     


Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp 
            980                 985                 990         


Arg Leu Ile Thr Gly Arg Leu Gln  Ser Leu Gln Thr Tyr  Val Thr Gln 
        995                 1000                 1005             


Gln Leu  Ile Arg Ala Ala Glu  Ile Arg Ala Ser Ala  Asn Leu Ala 
    1010                 1015                 1020             


Ala Thr  Lys Met Ser Glu Cys  Val Leu Gly Gln Ser  Lys Arg Val 
    1025                 1030                 1035             


Asp Phe  Cys Gly Lys Gly Tyr  His Leu Met Ser Phe  Pro Gln Ser 
    1040                 1045                 1050             


Ala Pro  His Gly Val Val Phe  Leu His Val Thr Tyr  Val Pro Ala 
    1055                 1060                 1065             


Gln Glu  Lys Asn Phe Thr Thr  Ala Pro Ala Ile Cys  His Asp Gly 
    1070                 1075                 1080             


Lys Ala  His Phe Pro Arg Glu  Gly Val Phe Val Ser  Asn Gly Thr 
    1085                 1090                 1095             


His Trp  Phe Val Thr Gln Arg  Asn Phe Tyr Glu Pro  Gln Ile Ile 
    1100                 1105                 1110             


Thr Thr  Asp Asn Thr Phe Val  Ser Gly Asn Cys Asp  Val Val Ile 
    1115                 1120                 1125             


Gly Ile  Val Asn Asn Thr Val  Tyr Asp Pro Leu Gln  Pro Glu Leu 
    1130                 1135                 1140             


Asp Ser  Phe Lys Glu Glu Leu  Asp Lys Tyr Phe Lys  Asn His Thr 
    1145                 1150                 1155             


Ser Pro  Asp Val Asp Leu Gly  Asp Ile Ser Gly Ile  Asn Ala Ser 
    1160                 1165                 1170             


Val Val  Asn Ile Gln Lys Glu  Ile Asp Arg Leu Asn  Glu Val Ala 
    1175                 1180                 1185             


Lys Asn  Leu Asn Glu Ser Leu  Ile Asp Leu Gln Glu  Leu Gly Lys 
    1190                 1195                 1200             


Tyr Glu  Gln Tyr Ile Lys Trp  Pro Trp Tyr Ile Trp  Leu Gly Phe 
    1205                 1210                 1215             


Ile Ala  Gly Leu Ile Ala Ile  Val Met Val Thr Ile  Met Leu Cys 
    1220                 1225                 1230             


Cys Met  Thr Ser Cys Cys Ser  Cys Leu Lys Gly Cys  Cys Ser Cys 
    1235                 1240                 1245             


Gly Ser  Cys Cys Lys Phe Asp  Glu Asp Asp Ser Glu  Pro Val Leu 
    1250                 1255                 1260             


Lys Gly  Val Lys Leu His Tyr  Thr 
    1265                 1270     


<210>  128
<211>  3816
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant B.1.351 (20H/501Y.V2)

<400>  128
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cttcaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgcc      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc ggggtctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

gccctgcaca gaagctacct gacacctggc gatagcagca gcggatggac agctggtgcc      780

gccgcttact atgtgggcta cctgcagcct agaaccttcc tgctgaagta caacgagaac      840

ggcaccatca ccgacgccgt ggattgtgct ctggatcctc tgagcgagac aaagtgcacc      900

ctgaagtcct tcaccgtgga aaagggcatc taccagacca gcaacttccg ggtgcagccc      960

accgaatcca tcgtgcggtt ccccaatatc accaatctgt gccccttcgg cgaggtgttc     1020

aatgccacca gattcgcctc tgtgtacgcc tggaaccgga agcggatcag caattgcgtg     1080

gccgactact ccgtgctgta caactccgcc agcttcagca ccttcaagtg ctacggcgtg     1140

tcccctacca agctgaacga cctgtgcttc acaaacgtgt acgccgacag cttcgtgatc     1200

cggggagatg aagtgcggca gattgcccct ggacagacag gcaacatcgc cgactacaac     1260

tacaagctgc ccgacgactt caccggctgt gtgattgcct ggaacagcaa caacctggac     1320

tccaaagtcg gcggcaacta caattacctg taccggctgt tccggaagtc caatctgaag     1380

cccttcgagc gggacatctc caccgagatc tatcaggccg gcagcacccc ttgtaacggc     1440

gtgaaaggct tcaactgcta cttcccactg cagtcctacg gctttcagcc cacatatggc     1500

gtgggctatc agccctacag agtggtggtg ctgagcttcg aactgctgca tgcccctgcc     1560

acagtgtgcg gccctaagaa aagcaccaat ctcgtgaaga acaaatgcgt gaacttcaac     1620

ttcaacggcc tgaccggcac cggcgtgctg acagagagca acaagaagtt cctgccattc     1680

cagcagtttg gccgggatat cgccgatacc acagacgccg ttagagatcc ccagacactg     1740

gaaatcctgg acatcacccc ttgcagcttc ggcggagtgt ctgtgatcac ccctggcacc     1800

aacaccagca atcaggtggc agtgctgtac cagggcgtga actgtaccga agtgcccgtg     1860

gccattcacg ccgatcagct gacacctaca tggcgggtgt actccaccgg cagcaatgtg     1920

tttcagacca gagccggctg tctgatcgga gccgagcacg tgaacaatag ctacgagtgc     1980

gacatcccca tcggcgctgg aatctgcgcc agctaccaga cacagacaaa cagccctcgg     2040

agagccagaa gcgtggccag ccagagcatc attgcctaca caatgtctct gggcgtcgag     2100

aacagcgtgg cctactccaa caactctatc gctatcccca ccaacttcac catcagcgtg     2160

accacagaga tcctgcctgt gtccatgacc aagaccagcg tggactgcac catgtacatc     2220

tgcggcgatt ccaccgagtg ctccaacctg ctgctgcagt acggcagctt ctgcacccag     2280

ctgaatagag ccctgacagg gatcgccgtg gaacaggaca agaacaccca agaggtgttc     2340

gcccaagtga agcagatcta caagacccct cctatcaagg acttcggcgg cttcaatttc     2400

agccagattc tgcccgatcc tagcaagccc agcaagcgga gcttcatcga ggacctgctg     2460

ttcaacaaag tgacactggc cgacgccggc ttcatcaagc agtatggcga ttgtctgggc     2520

gacattgccg ccagggatct gatttgcgcc cagaagttta acggactgac agtgctgcct     2580

cctctgctga ccgatgagat gatcgcccag tacacatctg ccctgctggc cggcacaatc     2640

acaagcggct ggacatttgg agcaggcgcc gctctgcaga tcccctttgc tatgcagatg     2700

gcctaccggt tcaacggcat cggagtgacc cagaatgtgc tgtacgagaa ccagaagctg     2760

atcgccaacc agttcaacag cgccatcggc aagatccagg acagcctgag cagcacagca     2820

agcgccctgg gaaagctgca ggacgtggtc aaccagaatg cccaggcact gaacaccctg     2880

gtcaagcagc tgtcctccaa cttcggcgcc atcagctctg tgctgaacga tatcctgagc     2940

agactggacc ctcctgaggc cgaggtgcag atcgacagac tgatcacagg cagactgcag     3000

agcctccaga catacgtgac ccagcagctg atcagagccg ccgagattag agcctctgcc     3060

aatctggccg ccaccaagat gtctgagtgt gtgctgggcc agagcaagag agtggacttt     3120

tgcggcaagg gctaccacct gatgagcttc cctcagtctg cccctcacgg cgtggtgttt     3180

ctgcacgtga catatgtgcc cgctcaagag aagaatttca ccaccgctcc agccatctgc     3240

cacgacggca aagcccactt tcctagagaa ggcgtgttcg tgtccaacgg cacccattgg     3300

ttcgtgacac agcggaactt ctacgagccc cagatcatca ccaccgacaa caccttcgtg     3360

tctggcaact gcgacgtcgt gatcggcatt gtgaacaata ccgtgtacga ccctctgcag     3420

cccgagctgg acagcttcaa agaggaactg gacaagtact ttaagaacca cacaagcccc     3480

gacgtggacc tgggcgatat cagcggaatc aatgccagcg tcgtgaacat ccagaaagag     3540

atcgaccggc tgaacgaggt ggccaagaat ctgaacgaga gcctgatcga cctgcaagaa     3600

ctggggaagt acgagcagta catcaagtgg ccctggtaca tctggctggg ctttatcgcc     3660

ggactgattg ccatcgtgat ggtcacaatc atgctgtgtt gcatgaccag ctgctgtagc     3720

tgcctgaagg gctgttgtag ctgtggcagc tgctgcaagt tcgacgagga cgattctgag     3780

cccgtgctga agggcgtgaa actgcactac acatga                               3816


<210>  129
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant B.1.427 & B.1.429 (CAL.20C)

<400>  129

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ile Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Cys Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  130
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant B.1.427 & B.1.429 (CAL.20C) DNA

<400>  130
atgttcgtgt tcctggtgct gctgcctctg gtgtccatcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctgcatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat taccggtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcttcaa ctgctacttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg gcgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  131
<211>  1271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant B.1.617.2

<400>  131

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Arg Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Asp Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Val Tyr Ser Ser 
145                 150                 155                 160 


Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp 
                165                 170                 175     


Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe 
            180                 185                 190         


Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile 
        195                 200                 205             


Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro Leu 
    210                 215                 220                 


Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu 
225                 230                 235                 240 


Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp 
                245                 250                 255     


Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr 
            260                 265                 270         


Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp 
        275                 280                 285             


Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe 
    290                 295                 300                 


Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro 
305                 310                 315                 320 


Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe 
                325                 330                 335     


Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn 
            340                 345                 350         


Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn 
        355                 360                 365             


Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys 
    370                 375                 380                 


Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile 
385                 390                 395                 400 


Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile 
                405                 410                 415     


Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile 
            420                 425                 430         


Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn 
        435                 440                 445             


Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg 
    450                 455                 460                 


Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro Cys Asn Gly 
465                 470                 475                 480 


Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln 
                485                 490                 495     


Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser 
            500                 505                 510         


Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser 
        515                 520                 525             


Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu 
    530                 535                 540                 


Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe 
545                 550                 555                 560 


Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp 
                565                 570                 575     


Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly 
            580                 585                 590         


Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val 
        595                 600                 605             


Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala 
    610                 615                 620                 


Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val 
625                 630                 635                 640 


Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn 
                645                 650                 655     


Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr 
            660                 665                 670         


Gln Thr Gln Thr Asn Ser Arg Arg Arg Ala Arg Ser Val Ala Ser Gln 
        675                 680                 685             


Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala 
    690                 695                 700                 


Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val 
705                 710                 715                 720 


Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys 
                725                 730                 735     


Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu 
            740                 745                 750         


Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile 
        755                 760                 765             


Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys 
    770                 775                 780                 


Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe 
785                 790                 795                 800 


Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile 
                805                 810                 815     


Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile 
            820                 825                 830         


Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile 
        835                 840                 845             


Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr 
    850                 855                 860                 


Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile 
865                 870                 875                 880 


Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe 
                885                 890                 895     


Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn 
            900                 905                 910         


Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala 
        915                 920                 925             


Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly 
    930                 935                 940                 


Lys Leu Gln Asn Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu 
945                 950                 955                 960 


Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn 
                965                 970                 975     


Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp 
            980                 985                 990         


Arg Leu Ile Thr Gly Arg Leu Gln  Ser Leu Gln Thr Tyr  Val Thr Gln 
        995                 1000                 1005             


Gln Leu  Ile Arg Ala Ala Glu  Ile Arg Ala Ser Ala  Asn Leu Ala 
    1010                 1015                 1020             


Ala Thr  Lys Met Ser Glu Cys  Val Leu Gly Gln Ser  Lys Arg Val 
    1025                 1030                 1035             


Asp Phe  Cys Gly Lys Gly Tyr  His Leu Met Ser Phe  Pro Gln Ser 
    1040                 1045                 1050             


Ala Pro  His Gly Val Val Phe  Leu His Val Thr Tyr  Val Pro Ala 
    1055                 1060                 1065             


Gln Glu  Lys Asn Phe Thr Thr  Ala Pro Ala Ile Cys  His Asp Gly 
    1070                 1075                 1080             


Lys Ala  His Phe Pro Arg Glu  Gly Val Phe Val Ser  Asn Gly Thr 
    1085                 1090                 1095             


His Trp  Phe Val Thr Gln Arg  Asn Phe Tyr Glu Pro  Gln Ile Ile 
    1100                 1105                 1110             


Thr Thr  Asp Asn Thr Phe Val  Ser Gly Asn Cys Asp  Val Val Ile 
    1115                 1120                 1125             


Gly Ile  Val Asn Asn Thr Val  Tyr Asp Pro Leu Gln  Pro Glu Leu 
    1130                 1135                 1140             


Asp Ser  Phe Lys Glu Glu Leu  Asp Lys Tyr Phe Lys  Asn His Thr 
    1145                 1150                 1155             


Ser Pro  Asp Val Asp Leu Gly  Asp Ile Ser Gly Ile  Asn Ala Ser 
    1160                 1165                 1170             


Val Val  Asn Ile Gln Lys Glu  Ile Asp Arg Leu Asn  Glu Val Ala 
    1175                 1180                 1185             


Lys Asn  Leu Asn Glu Ser Leu  Ile Asp Leu Gln Glu  Leu Gly Lys 
    1190                 1195                 1200             


Tyr Glu  Gln Tyr Ile Lys Trp  Pro Trp Tyr Ile Trp  Leu Gly Phe 
    1205                 1210                 1215             


Ile Ala  Gly Leu Ile Ala Ile  Val Met Val Thr Ile  Met Leu Cys 
    1220                 1225                 1230             


Cys Met  Thr Ser Cys Cys Ser  Cys Leu Lys Gly Cys  Cys Ser Cys 
    1235                 1240                 1245             


Gly Ser  Cys Cys Lys Phe Asp  Glu Asp Asp Ser Glu  Pro Val Leu 
    1250                 1255                 1260             


Lys Gly  Val Lys Leu His Tyr  Thr 
    1265                 1270     


<210>  132
<211>  3816
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant B.1.617.2

<400>  132
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgcgcacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctggacgtct actaccacaa gaacaacaag agctggatgg aaagcgaggt gtacagcagc      480

gccaacaact gcaccttcga gtacgtgtcc cagcctttcc tgatggacct ggaaggcaag      540

cagggcaact tcaagaacct gcgcgagttc gtgtttaaga acatcgacgg ctacttcaag      600

atctacagca agcacacccc tatcaacctc gtgcgggatc tgcctcaggg cttctctgct      660

ctggaacccc tggtggatct gcccatcggc atcaacatca cccggtttca gacactgctg      720

gccctgcaca gaagctacct gacacctggc gatagcagca gcggatggac agctggtgcc      780

gccgcttact atgtgggcta cctgcagcct agaaccttcc tgctgaagta caacgagaac      840

ggcaccatca ccgacgccgt ggattgtgct ctggatcctc tgagcgagac aaagtgcacc      900

ctgaagtcct tcaccgtgga aaagggcatc taccagacca gcaacttccg ggtgcagccc      960

accgaatcca tcgtgcggtt ccccaatatc accaatctgt gccccttcgg cgaggtgttc     1020

aatgccacca gattcgcctc tgtgtacgcc tggaaccgga agcggatcag caattgcgtg     1080

gccgactact ccgtgctgta caactccgcc agcttcagca ccttcaagtg ctacggcgtg     1140

tcccctacca agctgaacga cctgtgcttc acaaacgtgt acgccgacag cttcgtgatc     1200

cggggagatg aagtgcggca gattgcccct ggacagacag gcaagatcgc cgactacaac     1260

tacaagctgc ccgacgactt caccggctgt gtgattgcct ggaacagcaa caacctggac     1320

tccaaagtcg gcggcaacta caattaccgg taccggctgt tccggaagtc caatctgaag     1380

cccttcgagc gggacatctc caccgagatc tatcaggccg gcagcaaacc ttgtaacggc     1440

gtggaaggct tcaactgcta cttcccactg cagtcctacg gctttcagcc cacaaatggc     1500

gtgggctatc agccctacag agtggtggtg ctgagcttcg aactgctgca tgcccctgcc     1560

acagtgtgcg gccctaagaa aagcaccaat ctcgtgaaga acaaatgcgt gaacttcaac     1620

ttcaacggcc tgaccggcac cggcgtgctg acagagagca acaagaagtt cctgccattc     1680

cagcagtttg gccgggatat cgccgatacc acagacgccg ttagagatcc ccagacactg     1740

gaaatcctgg acatcacccc ttgcagcttc ggcggagtgt ctgtgatcac ccctggcacc     1800

aacaccagca atcaggtggc agtgctgtac cagggcgtga actgtaccga agtgcccgtg     1860

gccattcacg ccgatcagct gacacctaca tggcgggtgt actccaccgg cagcaatgtg     1920

tttcagacca gagccggctg tctgatcgga gccgagcacg tgaacaatag ctacgagtgc     1980

gacatcccca tcggcgctgg aatctgcgcc agctaccaga cacagacaaa cagccgtcgg     2040

agagccagaa gcgtggccag ccagagcatc attgcctaca caatgtctct gggcgccgag     2100

aacagcgtgg cctactccaa caactctatc gctatcccca ccaacttcac catcagcgtg     2160

accacagaga tcctgcctgt gtccatgacc aagaccagcg tggactgcac catgtacatc     2220

tgcggcgatt ccaccgagtg ctccaacctg ctgctgcagt acggcagctt ctgcacccag     2280

ctgaatagag ccctgacagg gatcgccgtg gaacaggaca agaacaccca agaggtgttc     2340

gcccaagtga agcagatcta caagacccct cctatcaagg acttcggcgg cttcaatttc     2400

agccagattc tgcccgatcc tagcaagccc agcaagcgga gcttcatcga ggacctgctg     2460

ttcaacaaag tgacactggc cgacgccggc ttcatcaagc agtatggcga ttgtctgggc     2520

gacattgccg ccagggatct gatttgcgcc cagaagttta acggactgac agtgctgcct     2580

cctctgctga ccgatgagat gatcgcccag tacacatctg ccctgctggc cggcacaatc     2640

acaagcggct ggacatttgg agcaggcgcc gctctgcaga tcccctttgc tatgcagatg     2700

gcctaccggt tcaacggcat cggagtgacc cagaatgtgc tgtacgagaa ccagaagctg     2760

atcgccaacc agttcaacag cgccatcggc aagatccagg acagcctgag cagcacagca     2820

agcgccctgg gaaagctgca gaacgtggtc aaccagaatg cccaggcact gaacaccctg     2880

gtcaagcagc tgtcctccaa cttcggcgcc atcagctctg tgctgaacga tatcctgagc     2940

agactggacc ctcctgaggc cgaggtgcag atcgacagac tgatcacagg cagactgcag     3000

agcctccaga catacgtgac ccagcagctg atcagagccg ccgagattag agcctctgcc     3060

aatctggccg ccaccaagat gtctgagtgt gtgctgggcc agagcaagag agtggacttt     3120

tgcggcaagg gctaccacct gatgagcttc cctcagtctg cccctcacgg cgtggtgttt     3180

ctgcacgtga catatgtgcc cgctcaagag aagaatttca ccaccgctcc agccatctgc     3240

cacgacggca aagcccactt tcctagagaa ggcgtgttcg tgtccaacgg cacccattgg     3300

ttcgtgacac agcggaactt ctacgagccc cagatcatca ccaccgacaa caccttcgtg     3360

tctggcaact gcgacgtcgt gatcggcatt gtgaacaata ccgtgtacga ccctctgcag     3420

cccgagctgg acagcttcaa agaggaactg gacaagtact ttaagaacca cacaagcccc     3480

gacgtggacc tgggcgatat cagcggaatc aatgccagcg tcgtgaacat ccagaaagag     3540

atcgaccggc tgaacgaggt ggccaagaat ctgaacgaga gcctgatcga cctgcaagaa     3600

ctggggaagt acgagcagta catcaagtgg ccctggtaca tctggctggg ctttatcgcc     3660

ggactgattg ccatcgtgat ggtcacaatc atgctgtgtt gcatgaccag ctgctgtagc     3720

tgcctgaagg gctgttgtag ctgtggcagc tgctgcaagt tcgacgagga cgattctgag     3780

cccgtgctga agggcgtgaa actgcactac acatga                               3816


<210>  133
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant B.1.617.1

<400>  133

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Gln Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Arg Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  His Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  134
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant B.1.617.1

<400>  134
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat taccggtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgc aaggcttcaa ctgctacttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg gcgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cgtcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct catgagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  135
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant B.1.526

<400>  135

Met Phe Val Phe Phe Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Ile Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Gly Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Val Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  136
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein S sequence of variant B.1.526

<400>  136
atgttcgtgt tcttcgtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcatcgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcggta gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtga aaggcttcaa ctgctacttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg gcgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggaatc tgcgccagct accagacaca gacaaacagc     2040

cctcggagag ccagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gtcgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagca ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacat ga                        3822


<210>  137
<211>  616
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ACE-2

<400>  137

Ala Asp Pro Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys 
1               5                   10                  15      


Phe Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser 
            20                  25                  30          


Trp Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn 
        35                  40                  45              


Asn Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu 
    50                  55                  60                  


Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu 
65                  70                  75                  80  


Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp 
                85                  90                  95      


Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr 
            100                 105                 110         


Ser Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu 
        115                 120                 125             


Leu Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn 
    130                 135                 140                 


Glu Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln 
145                 150                 155                 160 


Leu Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala 
                165                 170                 175     


Arg Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr 
            180                 185                 190         


Glu Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile 
        195                 200                 205             


Glu Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His 
    210                 215                 220                 


Leu His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr 
225                 230                 235                 240 


Ile Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp 
                245                 250                 255     


Gly Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln 
            260                 265                 270         


Lys Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp 
        275                 280                 285             


Ala Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly 
    290                 295                 300                 


Leu Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp 
305                 310                 315                 320 


Pro Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu 
                325                 330                 335     


Gly Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp 
            340                 345                 350         


Asp Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met 
        355                 360                 365             


Ala Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly 
    370                 375                 380                 


Phe His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro 
385                 390                 395                 400 


Lys His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp 
                405                 410                 415     


Asn Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val 
            420                 425                 430         


Gly Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val 
        435                 440                 445             


Phe Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu 
    450                 455                 460                 


Met Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu 
465                 470                 475                 480 


Thr Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser 
                485                 490                 495     


Phe Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu 
            500                 505                 510         


Ala Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp 
        515                 520                 525             


Ile Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg 
    530                 535                 540                 


Leu Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly 
545                 550                 555                 560 


Ala Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu 
                565                 570                 575     


Phe Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser 
            580                 585                 590         


Thr Asp Trp Ser Pro Tyr Ala Asp Gly Gly Gly Ser His His His His 
        595                 600                 605             


His His His His His His His His 
    610                 615     


<210>  138
<211>  1851
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ACE-2

<400>  138
gcggatccca gcactataga ggagcaagct aagacgttcc tcgataagtt taaccatgag       60

gcagaagatc tgttttacca gtcgagtctg gcatcctgga actataatac taacataacc      120

gaggagaacg tgcaaaacat gaataacgct ggagataagt ggagcgcctt tcttaaggaa      180

caatctacat tggcccagat gtatccactg caagaaattc aaaatctcac ggtcaagctc      240

cagctccaag cactccagca aaatggttct tcggttttgt cggaggataa gtctaaaagg      300

ttgaatacca tcctcaacac catgtctacg atttacagta ccggaaaggt ttgcaacccc      360

gacaacccac aggagtgcct gttgctcgag ccgggtttga acgaaattat ggcaaacagc      420

cttgactaca acgaacgcct ttgggcttgg gagtcttgga gatcagaggt aggaaaacag      480

cttagacctc tctatgagga atacgtggtc ctgaagaatg agatggctcg tgccaaccat      540

tacgaagatt atggtgatta ctggagagga gattacgaag tgaacggtgt ggatggctat      600

gactactcta gaggacaatt gatcgaggac gtggaacata cgtttgagga gattaagcct      660

ctgtatgaac accttcatgc ctatgtacgt gctaagctta tgaacgctta cccgtcgtac      720

atctccccga tcggatgcct tccggcccac ctgttgggag acatgtgggg caggttttgg      780

actaatctgt attctttgac agtacccttt ggacagaaac ctaacataga cgttacggat      840

gcgatggttg atcaagcatg ggacgctcaa agaattttca aagaagccga aaaatttttt      900

gtcagtgtcg gcctgccgaa tatgacccag ggcttttggg aaaacagtat gctgaccgat      960

cctggtaatg ttcagaaagc tgtatgccat cctaccgcat gggacctggg caaaggagat     1020

tttaggattc ttatgtgtac caaggttacg atggatgatt tccttaccgc tcatcacgag     1080

atgggtcaca tccagtacga catggcatat gccgctcagc ctttcctgct taggaacggc     1140

gccaacgagg gctttcacga ggcagtgggc gagattatgt ctctgtctgc ggctactccg     1200

aagcatttga aatctatagg cttgctttca cctgactttc aagaagataa cgaaacagag     1260

atcaatttcc tgcttaaaca agcgctgacg attgtaggaa ccctgccctt cacttacatg     1320

cttgaaaaat ggcgttggat ggtttttaag ggcgagattc ctaaagatca atggatgaaa     1380

aagtggtggg agatgaagcg cgagatagtc ggtgtagtcg aacccgtccc tcatgatgag     1440

acgtactgcg atcccgctag cttgttccat gtttccaacg attactcttt cattcgctat     1500

tacacaagga ccctgtatca atttcaattt caagaggcac tttgtcaggc tgcgaaacac     1560

gagggacctc tgcataagtg cgatatctcg aactctaccg aggcaggcca gaagttgttt     1620

aacatgctta gactgggcaa gagtgaacct tggacccttg ctttggaaaa cgtagtggga     1680

gccaagaaca tgaacgtgag gcccctgctc aactatttcg aacctttgtt tacttggctg     1740

aaagatcaaa acaaaaactc attcgttggt tggagcaccg attggagtcc ttacgcagat     1800

ggaggaggct cacaccatca tcaccaccat catcatcacc accatcactg a              1851


<210>  139
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  139
ttccggatta ttcataccgt cccac                                             25


<210>  140
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  140
cgttaacaat ggggaagctg tct                                               23


<210>  141
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  141
cttgggagtc ttggagatca gagg                                              24


<210>  142
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  142
caccgactat ctcgcgctt                                                    19


<210>  143
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal 3C cleavage site

<400>  143

Leu Glu Val Leu Phe Gln Gly Pro 
1               5               


<210>  144
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 1 wt 3c

<400>  144
ttccggatta ttcataccgt cccac                                             25


<210>  145
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 1 wt 3c

<400>  145
tggaccttga aacagaacct ccaggccgcc tcttgatttt tcgaa                       45


<210>  146
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 2 wt 3c

<400>  146
ggaggttctg tttcaaggtc caagggtcca gccaacagag                             40


<210>  147
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 2 wt 3c

<400>  147
cgttaacaat ggggaagctg tct                                               23


<210>  148
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 1 A475W

<400>  148
ttccggatta ttcataccgt cccac                                             25


<210>  149
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 1 A475W

<400>  149
cgttgcatgg ggttgagccc cattgatata tctcggtcga aatatctctt tcaaacg          57


<210>  150
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 2  A475W

<400>  150
cgtttgaaag agatatttcg accgagatat atcaatgggg ctcaacccca tgcaacg          57


<210>  151
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 2  A475W

<400>  151
cgttaacaat ggggaagctg tct                                               23


<210>  152
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 1 N-RBD + Y449A

<400>  152
ttccggatta ttcataccgt cccac                                             25


<210>  153
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 1 N-RBD + Y449A

<400>  153
gttggattta cgaaacagac gatacagata attcgcatta ccaccgacct tactatcaag       60

gt                                                                      62


<210>  154
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 2 N-RBD + Y449A

<400>  154
accttgatag taaggtcggt ggtaatgcga attatctgtt cgtctgtttc gtaaatccaa       60

c                                                                       61


<210>  155
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 2 N-RBD + Y449A

<400>  155
cgttaacaat ggggaagctg tct                                               23


<210>  156
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 1 C-RBD + Q493A

<400>  156
ttccggatta ttcataccgt cccac                                             25


<210>  157
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 1 C-RBD + Q493A

<400>  157
cgggcgcaaa accgtaactc gcgagcggaa agtaacaatt gaagcc                      46


<210>  158
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 2 C-RBD + Q493A

<400>  158
ggcttcaatt gttactttcc gctcgcgagt tacggttttg cgcccg                      46


<210>  159
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 2 C-RBD + Q493A

<400>  159
cgttaacaat ggggaagctg tct                                               23


<210>  160
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 1 N-RBD + C-RBD

<400>  160
ttccggatta ttcataccgt cccac                                             25


<210>  161
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 1 N-RBD + C-RBD

<400>  161
gcgcaaaacc gtaactctgg                                                   20


<210>  162
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 2 N-RBD + C-RBD

<400>  162
ccagagttac ggttttgcgc                                                   20


<210>  163
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 2 N-RBD + C-RBD

<400>  163
cgttaacaat ggggaagctg tct                                               23


<210>  164
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp1 F486A

<400>  164
ttccggatta ttcataccgt cccac                                             25


<210>  165
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv1 F486A

<400>  165
ctctggagcg gaaagtaaca attcgcgcct tcaacaccgt tgcatg                      46


<210>  166
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 2 F486A

<400>  166
catgcaacgg tgttgaaggc gcgaattgtt actttccgct ccagag                      46


<210>  167
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 2 F486A

<400>  167
cgttaacaat ggggaagctg tct                                               23


<210>  168
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 1 N487A

<400>  168
ttccggatta ttcataccgt cccac                                             25


<210>  169
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 1 N487A

<400>  169
gtaactctgg agcggaaagt aacacgcgaa gccttcaaca ccgttgc                     47


<210>  170
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 2 N487A

<400>  170
gcaacggtgt tgaaggcttc gcgtgttact ttccgctcca gagttac                     47


<210>  171
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 2 N487A

<400>  171
cgttaacaat ggggaagctg tct                                               23


<210>  172
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer  Fp 1 Y489A

<400>  172
ttccggatta ttcataccgt cccac                                             25


<210>  173
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer  Rv 1 Y489A

<400>  173
ccgtaactct ggagcggaaa cgcacaattg aagccttcaa caccg                       45


<210>  174
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer  Fp 2 Y489A

<400>  174
cggtgttgaa ggcttcaatt gtgcgtttcc gctccagagt tacgg                       45


<210>  175
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer  Rv 2 Y489A

<400>  175
cgttaacaat ggggaagctg tct                                               23


<210>  176
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 1 N487W

<400>  176
ttccggatta ttcataccgt cccac                                             25


<210>  177
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 1 N487W

<400>  177
cgtaactctg gagcggaaag taacaccaga agccttcaac accgttgc                    48


<210>  178
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Fp 2 N487W

<400>  178
gcaacggtgt tgaaggcttc tggtgttact ttccgctcca gagttacg                    48


<210>  179
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Rv 2 N487W

<400>  179
cgttaacaat ggggaagctg tct                                               23


<210>  180
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tag

<400>  180

Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Ser Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            20                  25                  


<210>  181
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  181

Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Trp Gly Ser Lys Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  182
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  182

Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Trp Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  183
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  183

Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  184
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  184

Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  185
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  185

Ala Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  186
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  186

Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Asn Gly Val Gly Ala 
    50                  55          


<210>  187
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  187

Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Asn Gly Val Gly Ala 
    50                  55          


<210>  188
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  188

Tyr Asn Tyr Arg Ala Arg Ala Ala Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Ala Ser Tyr Gly 
        35                  40                  45              


Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    50                  55          


<210>  189
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid

<400>  189

Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
1               5                   10                  15      


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro Cys 
            20                  25                  30          


Asn Gly Val Glu Gly Ala Ala Cys Ala Phe Pro Leu Gln Ser Tyr Gly 
        35                  40                  45              


Phe Ala Pro Ala Asn Gly Val Gly Ala 
    50                  55          


