                         SEQUENCE LISTING

<110>  Biofidelity Ltd
 
<120>  Polynucleotide Detection

<130>  P35134WO1

<150>  GB2109017.0
<151>  2021-06-23

<150>  GB2109111.1
<151>  2021-06-24

<160>  94    

<170>  PatentIn version 3.5

<210>  1
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  single-stranded first oligonucleotide 1


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  1
atgttcgatg agctttgaca atacttgaag ctcgcagata taggatgttg cgatagtcca       60

ggaggctgc                                                               69


<210>  2
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  single-stranded ligating oligonucleotide 2

<400>  2
tgtcaaagct catcgaacat cctggactat gtctcc                                 36


<210>  3
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  single-stranded oligonucleotide 3

<400>  3
tgctgggcat ctgcctcacc tccaccgtgc agctcatcac gcagctcatg cccttcggca       60

gcctcctgga ctatg                                                        75


<210>  4
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  single-stranded oligonucleotide 4

<400>  4
tgctgggcat ctgcctcacc tccaccgtgc agctcatcac gcagctcatg cccttcggct       60

gcctcctgga ctatg                                                        75


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 1

<400>  5
tcgcaacatc ctatatctgc                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 2

<400>  6
tgagctttga caatacttga                                                   20


<210>  7
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WT oligonucleotide

<400>  7
catctgcctc acctccaccg tgcagctcat cacgcagctc atgcccttcg gctgcctcct       60

ggactatgtc cgggaacaca aagacaatat                                        90


<210>  8
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T790M oligonucleotide

<400>  8
catctgcctc acctccaccg tgcagctcat catgcagctc atgcccttcg gctgcctcct       60

ggactatgtc cgggaacaca aagacaatat                                        90


<210>  9
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C797S_2389 oligonucleotide

<400>  9
catctgcctc acctccaccg tgcagctcat cacgcagctc atgcccttcg gcagcctcct       60

ggactatgtc cgggaacaca aagacaatat                                        90


<210>  10
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T790M probe


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  10
atgttcgatg agctttgaca atacttgagc acggcagata taggatgttg cgaagggcat       60

gagctgcatg atgagctg                                                     78


<210>  11
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C797S_2389 probe


<220>
<221>  /5Phos
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  11
atgttcgatg agctttgaca atacttgaag ctcgcagata taggatgttg cgatagtcca       60

ggaggctgc                                                               69


<210>  12
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T790M splint oligonucleotide

<400>  12
tgtcaaagct catcgaacat gcccttcgca acatct                                 36


<210>  13
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C797S_2389 splint oligonucleotide

<400>  13
tgtcaaagct catcgaacat tcctggacta tcgcat                                 36


<210>  14
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cy5 primer


<220>
<221>  /Qusar670/
<222>  (1)..(1)
<223>  Quasar670 dye

<220>
<221>  /BHQ2/
<222>  (50)..(50)
<223>  Black Hole Quencher

<400>  14
acgcctggtt accgagccag gttcgcacat gtaggctcgg taaccaggcg acatcctata       60

tctgccgtgc                                                              70


<210>  15
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  15	TexasRed primer


<220>
<221>  /TexasRed/
<222>  (1)..(1)
<223>  Texas Red dye

<220>
<221>  /BHQ2/
<222>  (38)..(38)
<223>  Black Hole Quencher

<400>  15
acgcctggtt acaggttcgc acatgtagta accaggcgca acatcctata tctgcgag         58


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  16
atgttcgatg agctttgaca                                                   20


<210>  17
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WT oligonucleotide

<400>  17
cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat caaagtgctg       60

ggctccggtg cgttcggcac ggtgtataag gtaaggtccc                            100


<210>  18
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G719X_6239 oligonucleotide

<400>  18
cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat caaagtgctg       60

gcctccggtg cgttcggcac ggtgtataag gtaaggtccc                            100


<210>  19
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  19	G719X_6252 oligonucleotide

<400>  19
cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat caaagtgctg       60

agctccggtg cgttcggcac ggtgtataag gtaaggtccc                            100


<210>  20
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G719X_6253 oligonucleotide

<400>  20
cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat caaagtgctg       60

tgctccggtg cgttcggcac ggtgtataag gtaaggtccc                            100


<210>  21
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G719X_6239 probe oligonucleotide


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  21
atgttcgatg agctttgaca atacttgaca tgcgcagata taggatgttg cgaaacgcac       60

cggaggccag cactttg                                                      77


<210>  22
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G719X_6252 probe oligonucleotide


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  22
atgttcgatg agctttgaca atacttgaca tgccgagtaa tgagagtttc gcaaacgcac       60

cggagctcag cactttg                                                      77


<210>  23
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G719X_6253 probe oligonucleotide


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  23
atgttcgatg agctttgaca atacttgaca tgcgagcaat taggtagtgt cgtaacgcac       60

cggagcacag cactttg                                                      77


<210>  24
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Splint oligonucleotide

<400>  24
tgtcaaagct catcgaacat ccggtgcgtt cggcaa                                 36


<210>  25
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dye primer 1


<220>
<221>  /5Cy5/
<222>  (1)..(1)
<223>  Cy5 dye on 5 end

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<400>  25
actgaccagc tccatgacaa tcgctgtcgc catgatcgat cgcaacatcc tatatctgc        59


<210>  26
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dye primer 2


<220>
<221>  /5TEX615/
<222>  (1)..(1)
<223>  TEX dye on 5 end

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<400>  26
actgaccagc tccatgacaa tcgctgtcgc catgatcgat gcgaaactct cattactcg        59


<210>  27
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dye primer 3


<220>
<221>  /5HEX615/
<222>  (1)..(1)
<223>  Hex dye on 5 end

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<400>  27
tacgaccgac tcactcctta cagcagtccg cagtatgcta cgacactacc taattgctc        59


<210>  28
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quencher primer 1


<220>
<221>  /3IAbRQSp/
<222>  (39)..(39)
<223>  3' Iowa Black RQ quencher

<400>  28
tcgatcatgg cgacagcgat tgtcatggag ctggtcagt                              39


<210>  29
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quencher primer 2


<220>
<221>  /3IAbkFQ/
<222>  (39)..(39)
<223>  3' Iowa Black FQ quencher

<400>  29
agcatactgc ggactgctgt aaggagtgag tcggtcgta                              39


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer


<220>
<221>  *
<222>  (1)..(2)
<223>  Phosphorothioate bonds between bases 1&2, 2&3

<400>  30
tgagctttga caatacttga                                                   20


<210>  31
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WT oligonucleotide

<400>  31
ctgctgggca tctgcctcac ctccaccgtg cagctcatca cgcagctcat gcccttcggc       60

tgcctcctgg actatgtccg g                                                 81


<210>  32
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant oligonucleotide

<400>  32
ctgctgggca tctgcctcac ctccaccgtg cagctcatca tgcagctcat gcccttcggc       60

tgcctcctgg actatgtccg g                                                 81


<210>  33
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe A0


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  33
agctgcatct gagctttgac aatacttgag cacggcagat ataggatgtt gcgaagggca       60

tgagctgcat gatg                                                         74


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix Fwd


<220>
<221>  *
<222>  (1)..(2)
<223>  Phosphorothioate bonds between bases 1&2, 2&3

<400>  34
tcgcaacatc ctatatctgc                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix Rev

<400>  35
atgttgcgaa gggcatatgt                                                   20


<210>  36
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Methylated oligonucleotide


<220>
<221>  /iMe-dC/
<222>  (51)..(51)
<223>  5-Methyl deoxycytidine

<220>
<221>  /iMe-dC/
<222>  (58)..(58)
<223>  5-Methyl deoxycytidine

<220>
<221>  /iMe-dC/
<222>  (62)..(62)
<223>  5-Methyl deoxycytidine

<220>
<221>  /iMe-dC/
<222>  (63)..(63)
<223>  5-Methyl deoxycytidine

<220>
<221>  /iMe-dC/
<222>  (65)..(65)
<223>  5-Methyl deoxycytidine

<220>
<221>  /iMe-dC/
<222>  (66)..(66)
<223>  5-Methyl deoxycytidine

<220>
<221>  /iMe-dC/
<222>  (71)..(71)
<223>  5-Methyl deoxycytidine

<220>
<221>  /iMe-dC/
<222>  (75)..(75)
<223>  5-Methyl deoxycytidine

<220>
<221>  /iMe-dC/
<222>  (78)..(78)
<223>  5-Methyl deoxycytidine

<400>  36
cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat caaagtgctg       60

gcctccggtg cgttcggcac ggtgtataag gtaaggtccc                            100


<210>  37
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse complementary unmethylated nucleotide

<400>  37
accttacctt atacaccgtg ccgaacgcac cggaggccag cactttgatc tttttgaatt       60

cagtttcctt                                                              70


<210>  38
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Unmethylated nucleotide

<400>  38
cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat caaagtgctg       60

gcctccggtg cgttcggcac ggtgtataag gtaaggtccc                            100


<210>  39
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe oligonucleotide


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  39
atgttcgatg agctttgaca atacttgaca tgcgcagata taggatgttg cgaaacgcac       60

cggaggccag cactttg                                                      77


<210>  40
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Splint oligonucleotide

<400>  40
tgtcaaagct catcgaacat ccggtgcgtt cggcaa                                 36


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 1 Fwd


<220>
<221>  *
<222>  (1)..(2)
<223>  Phosphorothioate bonds between bases 1&2, 2&3

<400>  41
tcgcaacatc ctatatctgc                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 1 Rev


<220>
<221>  *
<222>  (1)..(2)
<223>  Phosphorothioate bonds between bases 1&2, 2&3

<400>  42
tgagctttga caatacttga                                                   20


<210>  43
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Unmethylated oligonucleotide

<400>  43
ctcagcgtac ccttgtcccc aggaagcata cgtgatggca tacgtgatgg ctggtgtggg       60

ctccccatat gtctcccgcc ttctgggcat                                        90


<210>  44
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Methylated oligonucleotide


<220>
<221>  /iMe-dC/
<222>  (20)..(20)
<223>  5-Methyl deoxycytidine

<400>  44
ctcagcgtac ccttgtcccc aggaagcata cgtgatggca tacgtgatgg ctggtgtggg       60

ctccccatat gtctcccgcc ttctgggcat                                        90


<210>  45
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe oligonucleotide


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<220>
<221>  /3SpC3/
<222>  (93)..(93)
<223>  3' C3 Spacer

<400>  45
atgttcgatg agctttgaca atacttgatc gatgcagata taggatgttg cgaccatcac       60

gtatgccatc acgtatgctt cctggggaca ttt                                    93


<210>  46
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Splint oligonucleotide

<400>  46
tgtcaaagct cagctatctg acgtgattcg caacaa                                 36


<210>  47
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 FWD 1


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<400>  47
cccaaccaag ctctcttgag gatcttg                                           27


<210>  48
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 REV 1


<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  48
gggaccttac cttatacacc gtgccg                                            26


<210>  49
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 FWD 2


<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  49
gcctccctcg cgccatcaga aggtgagaaa gttaaaattc ccgtc                       45


<210>  50
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 REV 2


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<400>  50
gccttgccag cccgctcaga cagcaaagca gaaactcaca tcg                         43


<210>  51
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 FWD 3


<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  51
gcctccctcg cgccatcagt gcctcacctc caccgtgca                              39


<210>  52
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 REV 3


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<400>  52
gccttgccag cccgctcaga ttgtctttgt gttcccggac at                          42


<210>  53
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 FWD 4


<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  53
gaagccacac tgacgtgcct ctc                                               23


<210>  54
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 REV 4


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<400>  54
aggcagatgc ccagcaggcg gca                                               23


<210>  55
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 FWD 5


<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  55
cgtactggtg aaaacaccgc ag                                                22


<210>  56
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 REV 5


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<400>  56
ccttctgcat ggtattcttt ctcttcc                                           27


<210>  57
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 FWD 6


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<400>  57
gctgaaaatg actgaatata aacttgtggt agttg                                  35


<210>  58
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 REV 6


<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  58
tgattctgaa ttagctgtat cgtcaa                                            26


<210>  59
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 FWD 7


<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  59
ctggtccctc attgcactgt actcc                                             25


<210>  60
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 REV 7


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<400>  60
agaaacctgt ctcttggata ttctcgacac                                        30


<210>  61
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 FWD 8


<220>
<221>  /5Phos
<222>  (1)..(1)
<223>  5' end phosphorylation

<400>  61
gcctccctcg cgccatcaga aaatggatcc agacaactgt tcaaactgat g                51


<210>  62
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 REV 8


<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  62
gccttgccag cccgctcagt tcatgaagac ctcacagtaa aaataggtga tt               52


<210>  63
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 FWD 9


<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  63
gcccccagcc ctctgac                                                      17


<210>  64
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 REV 9


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<400>  64
gccgtcgctt gatgaggatc cc                                                22


<210>  65
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 FWD 10


<220>
<221>  *
<222>  (1)..(3)
<223>  Phosphorothioate bonds between bases 1&2, 2&3, 3&4

<400>  65
ttgtccccag gaagcatacg tg                                                22


<210>  66
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 2 REV 10


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<400>  66
atgcccagaa ggcgggagac at                                                22


<210>  67
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant oligonucleotide

<400>  67
ggccaccatg cgaagccaca ctgacgtgcc tctccctccc tccaggaagc ctacgtgatg       60

gccagcgtgg acaaccccca ccacgtgtgc cgcctgctgg gcatctgcct cacctccacc      120


<210>  68
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<220>
<221>  *
<222>  (63)..(63)
<223>  Phosphorothioate bond between bases 63&64

<400>  68
tggtggggtg agctttgaca atacttgatc ggccgagtaa tgagagtttc gcaggcggca       60

cacgtggtg                                                               69


<210>  69
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<220>
<221>  *
<222>  (62)..(62)
<223>  Phosphorothioate bond between bases 62&63

<400>  69
gtggtgggtg agctttgaca atacttgatc ggccgagtaa tgagagtttc gcaggcggca       60

cacgtggtg                                                               69


<210>  70
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<220>
<221>  *
<222>  (60)..(60)
<223>  Phosphorothioate bond between bases 60&61

<400>  70
acgtggtgtg agctttgaca atacttgatc ggccgagtaa tgagagtttc gcaggcggca       60

cacgtggtg                                                               69


<210>  71
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<220>
<221>  *
<222>  (61)..(61)
<223>  Phosphorothioate bond between bases 61&62

<400>  71
tggtggggtg agctttgaca atacttgatc ggccgagtaa tgagagtttc aggcggcaca       60

cgtggtg                                                                 67


<210>  72
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<220>
<221>  *
<222>  (60)..(60)
<223>  Phosphorothioate bond between bases 60&61

<400>  72
gtggtgggtg agctttgaca atacttgatc ggccgagtaa tgagagtttc aggcggcaca       60

cgtggtg                                                                 67


<210>  73
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<220>
<221>  *
<222>  (59)..(59)
<223>  Phosphorothioate bond between bases 59&60

<400>  73
cgtggtggtg agctttgaca atacttgatc ggccgagtaa tgagagtttc aggcggcaca       60

cgtggtg                                                                 67


<210>  74
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<220>
<221>  *
<222>  (63)..(63)
<223>  Phosphorothioate bond between bases 63&64

<400>  74
tggtggggtg agctttgaca atacttgatc ggccgagtaa tgagagtttc gcaggcggca       60

cacg                                                                    64


<210>  75
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<220>
<221>  *
<222>  (62)..(62)
<223>  Phosphorothioate bond between bases 62&63

<400>  75
gtggtgggtg agctttgaca atacttgatc ggccgagtaa tgagagtttc gcaggcggca       60

cac                                                                     63


<210>  76
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<220>
<221>  *
<222>  (61)..(61)
<223>  Phosphorothioate bond between bases 61&62

<400>  76
tggtggggtg agctttgaca atacttgatc ggccgagtaa tgagagtttc aggcggcaca       60

cg                                                                      62


<210>  77
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<220>
<221>  *
<222>  (60)..(60)
<223>  Phosphorothioate bond between bases 60&61

<400>  77
gtggtgggtg agctttgaca atacttgatc ggccgagtaa tgagagtttc aggcggcaca       60

c                                                                       61


<210>  78
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  /5Phos/
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<220>
<221>  *
<222>  (59)..(59)
<223>  Phosphorothioate bond between bases 59&60

<400>  78
cgtggtggtg agctttgaca atacttgatc ggccgagtaa tgagagtttc aggcggcaca       60


<210>  79
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 3 Primer 1


<220>
<221>  *
<222>  (1)..(2)
<223>  Phosphorothioate bonds between bases 1&2, 2&3

<400>  79
tgagctttga caatacttga                                                   20


<210>  80
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 3 Primer 2


<220>
<221>  /5Cy5/
<222>  (1)..(1)
<223>  Cy5 dye on 5 end

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<400>  80
actgaccagc tccatgacaa tcgctgtcgc catgatcgat cgcaacatcc tatatctgcg       60

c                                                                       61


<210>  81
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 3 Primer 3


<220>
<221>  /5TEX615/
<222>  (1)..(1)
<223>  TEX dye on 5 end

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<400>  81
actgaccagc tccatgacaa tcgctgtcgc catgatcgat gcgaaactct cattactcgg       60

c                                                                       61


<210>  82
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 3 Primer 4


<220>
<221>  /5HEX/
<222>  (1)..(1)
<223>  Hex dye on 5 end

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<400>  82
tacgaccgac tcactcctta cagcagtccg cagtatgcta cgacactacc taattgctcg       60

c                                                                       61


<210>  83
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 3 Primer 5


<220>
<221>  /5ATTO488N/
<222>  (1)..(1)
<223>  Atto488 dye on 5' end

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<400>  83
tacgaccgac tcactcctta cagcagtccg cagtatgctt cggtgatcag tcctcgatg        59


<210>  84
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 3 Primer 6


<220>
<221>  /3IAbRQSp/
<222>  (39)..(39)
<223>  3' Iowa Black RQ quencher

<400>  84
tcgatcatgg cgacagcgat tgtcatggag ctggtcagt                              39


<210>  85
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer mix 3 Primer 7


<220>
<221>  /3IAbkFQ/
<222>  (39)..(39)
<223>  3' Iowa Black FQ quencher

<400>  85
agcatactgc ggactgctgt aaggagtgag tcggtcgta                              39


<210>  86
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant oligonucleotide

<400>  86
gaagccacac tgacgtgcct ctccctccct ccaggaagcc ttccaggaag cctacgtgat       60

ggccagcgtg gacaaccccc acgtgtgccg cctgctgggc atctgcct                   108


<210>  87
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant oligonucleotide

<400>  87
acgtactggt gaaaacaccg cagcatgtca agatcacaga ttttgggcgt gccaaactgc       60

tgggtgcgga agagaaagaa taccatgcag aaggaggc                               98


<210>  88
<211>  109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant oligonucleotide

<400>  88
ccacaaaatg gatccagaca actgttcaaa ctgatgggac ccactccatc gagatttctc       60

tgtagctaga ccaaaatcac ctatttttac tgtgaggtct tcatgaaga                  109


<210>  89
<211>  122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant oligonucleotide

<400>  89
ctgtcatagg gactctggat cccagaaggt gagaaagtta aaattcccgt cgctatcaaa       60

acatctccga aagccaacaa ggaaatcctc gatgtgagtt tctgctttgc tgtgtggggg      120

tc                                                                     122


<210>  90
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  5Phos
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<220>
<221>  *
<222>  (65)..(65)
<223>  Phosphorothioate bond between bases 65&66

<400>  90
ggcttccttg agctttgaca atacttgatc ggcgcagata taggatgttg cgaaggcttc       60

ctggaa                                                                  66


<210>  91
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  5Phos
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<220>
<221>  *
<222>  (67)..(67)
<223>  Phosphorothioate bond between bases 67&68

<400>  91
cccaaaattg agctttgaca atacttgatc ggccgagtaa tgagagtttc gcacagcagt       60

ttggcacgcc caaaat                                                       76


<210>  92
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  5Phos
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<400>  92
tctgtagctg agctttgaca atacttgatc ggcgagcaat taggtagtgt cgtcatcgag       60

atttctctgt ag                                                           72


<210>  93
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe


<220>
<221>  5Phos
<222>  (1)..(1)
<223>  5' end phosphorylation

<220>
<221>  *
<222>  (1)..(1)
<223>  Phosphorothioate bond between bases 1&2

<400>  93
tgcagacttg agctttgaca atacttgatc gcatcgagga ctgatcaccg aagcggagat       60

gttttgatag c                                                            71


<210>  94
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Splint


<220>
<221>  3InvdT
<222>  (37)..(37)
<223>  3' end inverted dT

<400>  94
tgtcaaagct caagtctgca acatctccga atcgctt                                37


