               SEQUENCE LISTING

<110> CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

<120> MVA-BASED VACCINE EXPRESSING A PREFUSION-STABILIZED SARS-CoV-2 S PROTEIN

<130> 907 235

<150> EP21 383 245.4
<151> 2021-12-31
<150> EP21 382 557.3
<151> 2021-06-23

<160> 26

<170> BiSSAP 1.3.6

<210> 1
<211> 3830
<212> DNA
<213> Artificial Sequence


<220> 
<223> S nucleotide sequence

<400> 1
atgttcgtgt ttctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt tcaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcttcaa ctgctacttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggcatc tgtgccagct accagacaca gacaaacagc     2040

cccgggagtg caagttctgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagct ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acaccaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggacccacc tgaagccgag gtgcagatcg acagactgat caccggaagg     3000

ctgcagtccc tgcagaccta cgttacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata cgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacccagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggata agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacct gagcggccgc                3830


<210> 2
<211> 1273
<212> PRT
<213> Artificial Sequence


<220> 
<223> S protein sequence

<400> 2
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Ser Ser Val Ala 
        675                 680                 685             
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Pro Ser Ala 
    930                 935                 940                 
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 
        995                 1000                1005            
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu 
    1010                1015                1020                
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val 
1025                1030                1035                1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala 
                1045                1050                1055    
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu 
            1060                1065                1070        
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His 
        1075                1080                1085            
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val 
    1090                1095                1100                
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr 
1105                1110                1115                1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr 
                1125                1130                1135    
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu 
            1140                1145                1150        
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp 
        1155                1160                1165            
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp 
    1170                1175                1180                
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 
1185                1190                1195                1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile 
                1205                1210                1215    
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile 
            1220                1225                1230        
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys 
        1235                1240                1245            
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val 
    1250                1255                1260                
Leu Lys Gly Val Lys Leu His Tyr Thr 
1265                1270            

<210> 3
<211> 693
<212> DNA
<213> Artificial Sequence


<220> 
<223> RBD nucleotide sequence

<400> 3
atggaaaccg acacactgct gctgtgggtg ctgcttttgt gggtgccagg cagcacaggc      60

gattaccccg ggtctagatt ccccaacatc accaatctgt gccccttcgg cgaggtgttc     120

aatgccacaa gatttgccag cgtgtacgcc tggaaccgga agagaatcag caactgcgtg     180

gccgactaca gcgtgctgta caatagcgcc agcttcagca ccttcaagtg ctacggcgtg     240

tcccctacca agctgaacga cctgtgcttc accaatgtgt acgccgacag cttcgtgatc     300

agaggcgacg aagttcggca gatcgctcct ggacagacag gcaagatcgc cgattacaac     360

tacaagctgc ccgacgactt caccggctgc gtgatcgcct ggaatagcaa caacctggac     420

agcaaagtcg gcggcaacta caactacctg taccggctgt tccggaagtc caacctgaag     480

cctttcgagc gggacatcag caccgagatc tatcaggccg gcagcacccc ttgtaatggc     540

gtggaaggct tcaactgcta cttcccactg cagtcctacg gcttccagcc tacaaacggc     600

gtgggctacc agccttatag agtggtggtg ctgagcttcg aactgctgca tgcccctgct     660

accgtgtgtg gccctaagaa gtccaccaac tga                                  693


<210> 4
<211> 230
<212> PRT
<213> Artificial Sequence


<220> 
<223> RBD protein sequence

<400> 4
Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      
Gly Ser Thr Gly Asp Tyr Pro Gly Ser Arg Phe Pro Asn Ile Thr Asn 
            20                  25                  30          
Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
        35                  40                  45              
Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
    50                  55                  60                  
Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
65                  70                  75                  80  
Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
                85                  90                  95      
Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
            100                 105                 110         
Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
        115                 120                 125             
Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
    130                 135                 140                 
Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
145                 150                 155                 160 
Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
                165                 170                 175     
Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
            180                 185                 190         
Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
        195                 200                 205             
Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
    210                 215                 220                 
Pro Lys Lys Ser Thr Asn 
225                 230 

<210> 5
<211> 228
<212> DNA
<213> Artificial Sequence


<220> 
<223> E nucleotide sequence

<400> 5
atgtacagct tcgtgtccga ggaaaccggc acactgatcg tgaacagcgt gctgctgttc     60

ctggccttcg tggtgttcct gctggttacc ctggctatcc tgacagccct gagactgtgc    120

gcctactgct gcaacatcgt gaacgtgtcc ctcgtgaagc ccagcttcta cgtgtacagc    180

agagtgaaga acctgaactc cagcagggtg cccgacctgc tggtctaa                 228


<210> 6
<211> 75
<212> PRT
<213> Artificial Sequence


<220> 
<223> E protein sequence

<400> 6
Met Tyr Ser Phe Val Ser Glu Glu Thr Gly Thr Leu Ile Val Asn Ser 
1               5                   10                  15      
Val Leu Leu Phe Leu Ala Phe Val Val Phe Leu Leu Val Thr Leu Ala 
            20                  25                  30          
Ile Leu Thr Ala Leu Arg Leu Cys Ala Tyr Cys Cys Asn Ile Val Asn 
        35                  40                  45              
Val Ser Leu Val Lys Pro Ser Phe Tyr Val Tyr Ser Arg Val Lys Asn 
    50                  55                  60                  
Leu Asn Ser Ser Arg Val Pro Asp Leu Leu Val 
65                  70                  75  

<210> 7
<211> 1260
<212> DNA
<213> Artificial Sequence


<220> 
<223> N nucleotide sequence

<400> 7
atgagcgaca acggccctca gaatcagcgg aacgccccta gaatcacatt tggcggccct      60

agcgatagca ccggcagcaa tcagaatggc gagagaagcg gcgccagaag caaacagaga     120

aggcctcagg gcctgcctaa caacaccgcc tcttggttta cagccctgac acagcacggc     180

aaagaggacc tgaagtttcc tagaggccag ggcgtgccca tcaacaccaa tagcagccct     240

gatgaccaga tcggctacta cagacgggcc accagaagaa ttagaggcgg cgacggcaag     300

atgaaggatc tgagccccag atggtacttc tactacctcg gaacaggacc cgaggccgga     360

cttccttatg gcgccaacaa ggatggcatc atctgggttg caacagaagg cgccctgaac     420

acccctaagg accacatcgg aaccagaaat cccgccaaca atgccgccat tgtgctgcag     480

ttgccacagg gcacaaccct gcctaagggc ttttatgccg agggctctag aggcggaagc     540

caggcctcta gcagaagcag cagcagaagc cggaactcca gcagaaatag cacccctgga     600

agctccagag gcacatctcc tgccagaatg gccggaaatg gcggagatgc tgctcttgct     660

ctgctgctcc tggatcggct gaaccagctg gaaagcaaga tgtctggaaa gggccagcag     720

caacagggcc agaccgtgac aaaaaaatct gccgccgagg ccagcaagaa gcccagacag     780

aagagaaccg ccaccaaggc ctacaacgtg acccaggcct ttggaagaag aggcccagag     840

cagacccagg gcaatttcgg cgatcaagag ctgatcagac agggcaccga ctacaagcac     900

tggcctcaga ttgcccagtt tgcccctagc gcctccgcct tctttggcat gtctagaatc     960

ggcatggaag tgacccctag cggcacctgg ttgacataca caggcgccat caagctggac    1020

gacaaggacc ccaacttcaa ggaccaagtg atcctgctga acaagcacat cgacgcctac    1080

aagacattcc ctccaaccga gcctaagaag gacaagaaga agaaggccga cgagacacag    1140

gccctgccac agagacagaa aaaacagcag acagtgaccc tgctgcctgc cgctgatctg    1200

gacgattttt ccaagcagct gcagcagtcc atgagcagcg cagatagcac acaggcctga    1260


<210> 8
<211> 419
<212> PRT
<213> Artificial Sequence


<220> 
<223> N protein sequence

<400> 8
Met Ser Asp Asn Gly Pro Gln Asn Gln Arg Asn Ala Pro Arg Ile Thr 
1               5                   10                  15      
Phe Gly Gly Pro Ser Asp Ser Thr Gly Ser Asn Gln Asn Gly Glu Arg 
            20                  25                  30          
Ser Gly Ala Arg Ser Lys Gln Arg Arg Pro Gln Gly Leu Pro Asn Asn 
        35                  40                  45              
Thr Ala Ser Trp Phe Thr Ala Leu Thr Gln His Gly Lys Glu Asp Leu 
    50                  55                  60                  
Lys Phe Pro Arg Gly Gln Gly Val Pro Ile Asn Thr Asn Ser Ser Pro 
65                  70                  75                  80  
Asp Asp Gln Ile Gly Tyr Tyr Arg Arg Ala Thr Arg Arg Ile Arg Gly 
                85                  90                  95      
Gly Asp Gly Lys Met Lys Asp Leu Ser Pro Arg Trp Tyr Phe Tyr Tyr 
            100                 105                 110         
Leu Gly Thr Gly Pro Glu Ala Gly Leu Pro Tyr Gly Ala Asn Lys Asp 
        115                 120                 125             
Gly Ile Ile Trp Val Ala Thr Glu Gly Ala Leu Asn Thr Pro Lys Asp 
    130                 135                 140                 
His Ile Gly Thr Arg Asn Pro Ala Asn Asn Ala Ala Ile Val Leu Gln 
145                 150                 155                 160 
Leu Pro Gln Gly Thr Thr Leu Pro Lys Gly Phe Tyr Ala Glu Gly Ser 
                165                 170                 175     
Arg Gly Gly Ser Gln Ala Ser Ser Arg Ser Ser Ser Arg Ser Arg Asn 
            180                 185                 190         
Ser Ser Arg Asn Ser Thr Pro Gly Ser Ser Arg Gly Thr Ser Pro Ala 
        195                 200                 205             
Arg Met Ala Gly Asn Gly Gly Asp Ala Ala Leu Ala Leu Leu Leu Leu 
    210                 215                 220                 
Asp Arg Leu Asn Gln Leu Glu Ser Lys Met Ser Gly Lys Gly Gln Gln 
225                 230                 235                 240 
Gln Gln Gly Gln Thr Val Thr Lys Lys Ser Ala Ala Glu Ala Ser Lys 
                245                 250                 255     
Lys Pro Arg Gln Lys Arg Thr Ala Thr Lys Ala Tyr Asn Val Thr Gln 
            260                 265                 270         
Ala Phe Gly Arg Arg Gly Pro Glu Gln Thr Gln Gly Asn Phe Gly Asp 
        275                 280                 285             
Gln Glu Leu Ile Arg Gln Gly Thr Asp Tyr Lys His Trp Pro Gln Ile 
    290                 295                 300                 
Ala Gln Phe Ala Pro Ser Ala Ser Ala Phe Phe Gly Met Ser Arg Ile 
305                 310                 315                 320 
Gly Met Glu Val Thr Pro Ser Gly Thr Trp Leu Thr Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Leu Asp Asp Lys Asp Pro Asn Phe Lys Asp Gln Val Ile Leu 
            340                 345                 350         
Leu Asn Lys His Ile Asp Ala Tyr Lys Thr Phe Pro Pro Thr Glu Pro 
        355                 360                 365             
Lys Lys Asp Lys Lys Lys Lys Ala Asp Glu Thr Gln Ala Leu Pro Gln 
    370                 375                 380                 
Arg Gln Lys Lys Gln Gln Thr Val Thr Leu Leu Pro Ala Ala Asp Leu 
385                 390                 395                 400 
Asp Asp Phe Ser Lys Gln Leu Gln Gln Ser Met Ser Ser Ala Asp Ser 
                405                 410                 415     
Thr Gln Ala 
            

<210> 9
<211> 669
<212> DNA
<213> Artificial Sequence


<220> 
<223> M nucleotide sequence

<400> 9
atggccgata gcaatggcac catcaccgtg gaagaactga agaaactgct ggaacagtgg      60

aacctcgtga tcggcttcct gttcctgacc tggatctgcc tgctgcagtt cgcctacgcc     120

aaccggaaca gattcctgta tatcatcaag ctgatctttc tgtggctgct gtggcccgtg     180

acactggcct gttttgtgct ggccgccgtg tacagaatca actggatcac cggcggaatc     240

gccattgcca tggcttgtct tgtgggcctg atgtggctga gctactttat cgccagcttc     300

cggctgttcg cccggaccag atccatgtgg tccttcaatc ccgagacaaa catcctgctg     360

aacgtgcccc tgcacggcac cattctgaca agacctctgc tggaaagcga gctggttatc     420

ggcgccgtga tcctgagagg acacctgaga attgccggcc accacctggg cagatgcgat     480

atcaaggacc tgcctaaaga aatcacagtg gccaccagca gaaccctgtc ctactataag     540

ctgggcgcct ctcagagagt ggccggcgat agcggatttg ccgcctacag cagataccgg     600

atcggcaact acaagctgaa caccgaccac agctccagca gcgacaatat cgcactgctg     660

gtgcagtga                                                             669


<210> 10
<211> 222
<212> PRT
<213> Artificial Sequence


<220> 
<223> M protein sequence

<400> 10
Met Ala Asp Ser Asn Gly Thr Ile Thr Val Glu Glu Leu Lys Lys Leu 
1               5                   10                  15      
Leu Glu Gln Trp Asn Leu Val Ile Gly Phe Leu Phe Leu Thr Trp Ile 
            20                  25                  30          
Cys Leu Leu Gln Phe Ala Tyr Ala Asn Arg Asn Arg Phe Leu Tyr Ile 
        35                  40                  45              
Ile Lys Leu Ile Phe Leu Trp Leu Leu Trp Pro Val Thr Leu Ala Cys 
    50                  55                  60                  
Phe Val Leu Ala Ala Val Tyr Arg Ile Asn Trp Ile Thr Gly Gly Ile 
65                  70                  75                  80  
Ala Ile Ala Met Ala Cys Leu Val Gly Leu Met Trp Leu Ser Tyr Phe 
                85                  90                  95      
Ile Ala Ser Phe Arg Leu Phe Ala Arg Thr Arg Ser Met Trp Ser Phe 
            100                 105                 110         
Asn Pro Glu Thr Asn Ile Leu Leu Asn Val Pro Leu His Gly Thr Ile 
        115                 120                 125             
Leu Thr Arg Pro Leu Leu Glu Ser Glu Leu Val Ile Gly Ala Val Ile 
    130                 135                 140                 
Leu Arg Gly His Leu Arg Ile Ala Gly His His Leu Gly Arg Cys Asp 
145                 150                 155                 160 
Ile Lys Asp Leu Pro Lys Glu Ile Thr Val Ala Thr Ser Arg Thr Leu 
                165                 170                 175     
Ser Tyr Tyr Lys Leu Gly Ala Ser Gln Arg Val Ala Gly Asp Ser Gly 
            180                 185                 190         
Phe Ala Ala Tyr Ser Arg Tyr Arg Ile Gly Asn Tyr Lys Leu Asn Thr 
        195                 200                 205             
Asp His Ser Ser Ser Ser Asp Asn Ile Ala Leu Leu Val Gln 
    210                 215                 220         

<210> 11
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Myc A (F)

<400> 11
ggcgaatggg tgagtaacac g                                              21


<210> 12
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Myc B (R)

<400> 12
cggataacgc tgcgacctat g                                              21


<210> 13
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> TK-L (F)

<400> 13
tgattagttt gatgcgattc                                                20


<210> 14
<211> 17
<212> DNA
<213> Artificial Sequence


<220> 
<223> TK-R (R)

<400> 14
tgtccttgat acggcag                                                   17


<210> 15
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2-S-F

<400> 15
agctcgaggc caccatgttc gtgtttctgg tgctgc                               36


<210> 16
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2-S-F1

<400> 16
ggtttcagac actgctggcc ctgcacagaa gc                                   32


<210> 17
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2-S-F2

<400> 17
gcagtcctac ggctttcagc ccacaaatgg cg                                   32


<210> 18
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2-S-R2

<400> 18
atccctgtca gggctctatt cagctgggtg cag                                  33


<210> 19
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2-S-R1

<400> 19
tctggcccag cacacactca gacatcttgg tggcgg                               36


<210> 20
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2-S-R

<400> 20
aggcggccgc tcaggtgtag tgcagtttca cgccc                                35


<210> 21
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> Synthetic early/late promoter

<400> 21
aaaattgaaa ttttattttt tttttttgga atataaatag ctagctcgag                50


<210> 22
<400> 22
000

<210> 23
<211> 3886
<212> DNA
<213> Artificial Sequence


<220> 
<223> Full locus inserted in the MVA vector

<400> 23
aaaattgaaa ttttattttt tttttttgga atataaatag ctagctcgag gccaccatgt       60

tcgtgtttct ggtgctgctg cctctggtgt ccagccagtg tgtgaacctg accaccagaa      120

cacagctgcc tccagcctac accaacagct ttaccagagg cgtgtactac cccgacaagg      180

tgttcagatc cagcgtgctg cactctaccc aggacctgtt cctgcctttc ttcagcaacg      240

tgacctggtt ccacgccatc cacgtgtccg gcaccaatgg caccaagaga ttcgacaacc      300

ccgtgctgcc cttcaacgac ggggtgtact ttgccagcac cgagaagtcc aacatcatca      360

gaggctggat cttcggcacc acactggaca gcaagaccca gagcctgctg atcgtgaaca      420

acgccaccaa cgtggtcatc aaagtgtgcg agttccagtt ctgcaacgac cccttcctgg      480

gcgtctacta ccacaagaac aacaagagct ggatggaaag cgagttccgg gtgtacagca      540

gcgccaacaa ctgcaccttc gagtacgtgt cccagccttt cctgatggac ctggaaggca      600

agcagggcaa cttcaagaac ctgcgcgagt tcgtgttcaa gaacatcgac ggctacttca      660

agatctacag caagcacacc cctatcaacc tcgtgcggga tctgcctcag ggcttctctg      720

ctctggaacc cctggtggat ctgcccatcg gcatcaacat cacccggttt cagacactgc      780

tggccctgca cagaagctac ctgacacctg gcgatagcag cagcggatgg acagctggtg      840

ccgccgctta ctatgtgggc tacctgcagc ctagaacctt cctgctgaag tacaacgaga      900

acggcaccat caccgacgcc gtggattgtg ctctggatcc tctgagcgag acaaagtgca      960

ccctgaagtc cttcaccgtg gaaaagggca tctaccagac cagcaacttc cgggtgcagc     1020

ccaccgaatc catcgtgcgg ttccccaata tcaccaatct gtgccccttc ggcgaggtgt     1080

tcaatgccac cagattcgcc tctgtgtacg cctggaaccg gaagcggatc agcaattgcg     1140

tggccgacta ctccgtgctg tacaactccg ccagcttcag caccttcaag tgctacggcg     1200

tgtcccctac caagctgaac gacctgtgct tcacaaacgt gtacgccgac agcttcgtga     1260

tccggggaga tgaagtgcgg cagattgccc ctggacagac aggcaagatc gccgactaca     1320

actacaagct gcccgacgac ttcaccggct gtgtgattgc ctggaacagc aacaacctgg     1380

actccaaagt cggcggcaac tacaattacc tgtaccggct gttccggaag tccaatctga     1440

agcccttcga gcgggacatc tccaccgaga tctatcaggc cggcagcacc ccttgtaacg     1500

gcgtggaagg cttcaactgc tacttcccac tgcagtccta cggctttcag cccacaaatg     1560

gcgtgggcta tcagccctac agagtggtgg tgctgagctt cgaactgctg catgcccctg     1620

ccacagtgtg cggccctaag aaaagcacca atctcgtgaa gaacaaatgc gtgaacttca     1680

acttcaacgg cctgaccggc accggcgtgc tgacagagag caacaagaag ttcctgccat     1740

tccagcagtt tggccgggat atcgccgata ccacagacgc cgttagagat ccccagacac     1800

tggaaatcct ggacatcacc ccttgcagct tcggcggagt gtctgtgatc acccctggca     1860

ccaacaccag caatcaggtg gcagtgctgt accaggacgt gaactgtacc gaagtgcccg     1920

tggccattca cgccgatcag ctgacaccta catggcgggt gtactccacc ggcagcaatg     1980

tgtttcagac cagagccggc tgtctgatcg gagccgagca cgtgaacaat agctacgagt     2040

gcgacatccc catcggcgct ggcatctgtg ccagctacca gacacagaca aacagccccg     2100

ggagtgcaag ttctgtggcc agccagagca tcattgccta cacaatgtct ctgggcgccg     2160

agaacagcgt ggcctactcc aacaactcta tcgctatccc caccaacttc accatcagcg     2220

tgaccacaga gatcctgcct gtgtccatga ccaagaccag cgtggactgc accatgtaca     2280

tctgcggcga ttccaccgag tgctccaacc tgctgctgca gtacggcagc ttctgcaccc     2340

agctgaatag agccctgaca gggatcgccg tggaacagga caagaacacc caagaggtgt     2400

tcgcccaagt gaagcagatc tacaagaccc ctcctatcaa ggacttcggc ggcttcaatt     2460

tcagccagat tctgcccgat cctagcaagc ccagcaagcg gagcttcatc gaggacctgc     2520

tgttcaacaa agtgacactg gccgacgccg gcttcatcaa gcagtatggc gattgtctgg     2580

gcgacattgc cgccagggat ctgatttgcg cccagaagtt taacggactg acagtgctgc     2640

ctcctctgct gaccgatgag atgatcgccc agtacacatc tgccctgctg gccggcacaa     2700

tcacaagcgg ctggacattt ggagctggcg ccgctctgca gatccccttt gctatgcaga     2760

tggcctaccg gttcaacggc atcggagtga cccagaatgt gctgtacgag aaccagaagc     2820

tgatcgccaa ccagttcaac agcgccatcg gcaagatcca ggacagcctg agcagcacac     2880

caagcgccct gggaaagctg caggacgtgg tcaaccagaa tgcccaggca ctgaacaccc     2940

tggtcaagca gctgtcctcc aacttcggcg ccatcagctc tgtgctgaac gatatcctga     3000

gcagactgga cccacctgaa gccgaggtgc agatcgacag actgatcacc ggaaggctgc     3060

agtccctgca gacctacgtt acccagcagc tgatcagagc cgccgagatt agagcctctg     3120

ccaatctggc cgccaccaag atgtctgagt gtgtgctggg ccagagcaag agagtggact     3180

tttgcggcaa gggctaccac ctgatgagct tccctcagtc tgcccctcac ggcgtggtgt     3240

ttctgcacgt gacatacgtg cccgctcaag agaagaattt caccaccgct ccagccatct     3300

gccacgacgg caaagcccac tttcctagag aaggcgtgtt cgtgtccaac ggcacccatt     3360

ggttcgtgac ccagcggaac ttctacgagc cccagatcat caccaccgac aacaccttcg     3420

tgtctggcaa ctgcgacgtc gtgatcggca ttgtgaacaa taccgtgtac gaccctctgc     3480

agcccgagct ggacagcttc aaagaggaac tggataagta ctttaagaac cacacaagcc     3540

ccgacgtgga cctgggcgat atcagcggaa tcaatgccag cgtcgtgaac atccagaaag     3600

agatcgaccg gctgaacgag gtggccaaga atctgaacga gagcctgatc gacctgcaag     3660

aactggggaa gtacgagcag tacatcaagt ggccctggta catctggctg ggctttatcg     3720

ccggactgat tgccatcgtg atggtcacaa tcatgctgtg ttgcatgacc agctgctgta     3780

gctgcctgaa gggctgttgt agctgtggca gctgctgcaa gttcgacgag gacgattctg     3840

agcccgtgct gaagggcgtg aaactgcact acacctgagc ggccgc                    3886


<210> 24
<211> 573
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleotide sequence of the E3L gene of MVA (MVA050L)

<400> 24
atgtctaaga tctatattga cgagcgttct gacgcagaga ttgtgtgtgc ggctattaaa      60

aacattggaa tcgaaggagc tactgctgca caactaacta gacaacttaa tatggagaag     120

cgagaagtta ataaagctct gtacgatctt caacgtagtg ctatggtgta cagctccgac     180

gatattcctc ctcgttggtt tatgacaacg gaggcggata agccggatgc tgatgttatg     240

gctgacgcca taatagatga tgtatcccgc gaaaaatcaa tgagagagga tcataagtct     300

tttgatgatg ttattccggc taaaaaaatt attgattgga aagatgctaa ccctgtcacc     360

attattaatg agtactgcca aataactaag agagattggt cttttcgtat tgaatcagtg     420

gggcctagta actctcctac attttatgcc tgtgtagata tcgacggaag agtattcgat     480

aaggcagatg gaaaatctaa acgagatgct aaaaataatg cagctaaatt ggcagtagat     540

aaacttcttg gttacgtcat cattagattc tga                                  573


<210> 25
<211> 190
<212> PRT
<213> Artificial Sequence


<220> 
<223> MVA E3 protein sequence

<400> 25
Met Ser Lys Ile Tyr Ile Asp Glu Arg Ser Asp Ala Glu Ile Val Cys 
1               5                   10                  15      
Ala Ala Ile Lys Asn Ile Gly Ile Glu Gly Ala Thr Ala Ala Gln Leu 
            20                  25                  30          
Thr Arg Gln Leu Asn Met Glu Lys Arg Glu Val Asn Lys Ala Leu Tyr 
        35                  40                  45              
Asp Leu Gln Arg Ser Ala Met Val Tyr Ser Ser Asp Asp Ile Pro Pro 
    50                  55                  60                  
Arg Trp Phe Met Thr Thr Glu Ala Asp Lys Pro Asp Ala Asp Val Met 
65                  70                  75                  80  
Ala Asp Ala Ile Ile Asp Asp Val Ser Arg Glu Lys Ser Met Arg Glu 
                85                  90                  95      
Asp His Lys Ser Phe Asp Asp Val Ile Pro Ala Lys Lys Ile Ile Asp 
            100                 105                 110         
Trp Lys Asp Ala Asn Pro Val Thr Ile Ile Asn Glu Tyr Cys Gln Ile 
        115                 120                 125             
Thr Lys Arg Asp Trp Ser Phe Arg Ile Glu Ser Val Gly Pro Ser Asn 
    130                 135                 140                 
Ser Pro Thr Phe Tyr Ala Cys Val Asp Ile Asp Gly Arg Val Phe Asp 
145                 150                 155                 160 
Lys Ala Asp Gly Lys Ser Lys Arg Asp Ala Lys Asn Asn Ala Ala Lys 
                165                 170                 175     
Leu Ala Val Asp Lys Leu Leu Gly Tyr Val Ile Ile Arg Phe 
            180                 185                 190 

<210> 26
<211> 39
<212> DNA
<213> Artificial Sequence


<220> 
<223> synthetic early/late promoter without spacer

<400> 26
aaaattgaaa ttttattttt tttttttgga atataaata                            39


