                         SEQUENCE LISTING

<110>  Wuhan Institute of Virology, Chinese Academy of Sciences
 
<120>  ANTEROGRADE MONOSYNAPTIC TRANSNEURONAL TRACER SYSTEM WITH GK 
       PROTEIN FUNCTION MANIPULATION

<130>  1003.P003

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1017
<212>  DNA
<213>  herpes simplex virus 1

<400>  1
atgctcgccg tccgttccct gcagcacctc tcaaccgtcg tcttgataac ggcgtacggc       60

ctcgtgctcg tgtggtacac cgtcttcggt gccagtccgc tgcaccgatg tatttacgcg      120

gtacgcccca ccggcaccaa caacgacacc gccctcgtgt ggatgaaaat gaaccagacc      180

ctattgtttc tgggggcccc gacgcacccc cccaacgggg gctggcgcaa ccacgcccat      240

atctgctacg ccaatcttat cgcgggtagg gtcgtgccct tccaggtccc acccgacgcc      300

atgaatcgtc ggatcatgaa cgtccacgag gcagttaact gtctggagac cctatggtac      360

acacgggtgc gtctggtggt cgtagggtgg ttcctgtatc tggcgttcgt cgccctccac      420

caacgccgat gtatgtttgg tgtcgtgagt cccgcccaca agatggtggc cccggccacc      480

tacctcttga actacgcagg ccgcatcgta tcgagcgtgt tcctgcagta cccctacacg      540

aaaattaccc gcctgctctg cgagctgtcg gtccagcgac agaacctggt tcagttgttt      600

gagacggacc cggtcacctt cttgtaccac cgccccgcca tcggggtcat cgtaggctgc      660

gagttgatgc tacgctttgt ggccgtgggt ctcatcgtcg gcaccgcttt catatcccgt      720

ggggcatgtg cgatcacata ccccctgttt ctgaccatca ccacctggtg ttttgtctcc      780

accatcggcc tgacagagct gtattgtatt ctgcggcggg gcccggcccc caagaacgca      840

gacaaggccg ccgccccggg gcgatccaag gggctgtcgg gcgtctgcgg gcgctgttgt      900

tccatcatcc tctcgggcat cgcagtgcga ttgtgttata tcgccgtggt ggccggggtg      960

gtgctcgtgg cgcttcacta cgagcaggag atccagaggc gcctgtttga tgtatga        1017


<210>  2
<211>  338
<212>  PRT
<213>  herpes simplex virus 1

<400>  2

Met Leu Ala Val Arg Ser Leu Gln His Leu Ser Thr Val Val Leu Ile 
1               5                   10                  15      


Thr Ala Tyr Gly Leu Val Leu Val Trp Tyr Thr Val Phe Gly Ala Ser 
            20                  25                  30          


Pro Leu His Arg Cys Ile Tyr Ala Val Arg Pro Thr Gly Thr Asn Asn 
        35                  40                  45              


Asp Thr Ala Leu Val Trp Met Lys Met Asn Gln Thr Leu Leu Phe Leu 
    50                  55                  60                  


Gly Ala Pro Thr His Pro Pro Asn Gly Gly Trp Arg Asn His Ala His 
65                  70                  75                  80  


Ile Cys Tyr Ala Asn Leu Ile Ala Gly Arg Val Val Pro Phe Gln Val 
                85                  90                  95      


Pro Pro Asp Ala Met Asn Arg Arg Ile Met Asn Val His Glu Ala Val 
            100                 105                 110         


Asn Cys Leu Glu Thr Leu Trp Tyr Thr Arg Val Arg Leu Val Val Val 
        115                 120                 125             


Gly Trp Phe Leu Tyr Leu Ala Phe Val Ala Leu His Gln Arg Arg Cys 
    130                 135                 140                 


Met Phe Gly Val Val Ser Pro Ala His Lys Met Val Ala Pro Ala Thr 
145                 150                 155                 160 


Tyr Leu Leu Asn Tyr Ala Gly Arg Ile Val Ser Ser Val Phe Leu Gln 
                165                 170                 175     


Tyr Pro Tyr Thr Lys Ile Thr Arg Leu Leu Cys Glu Leu Ser Val Gln 
            180                 185                 190         


Arg Gln Asn Leu Val Gln Leu Phe Glu Thr Asp Pro Val Thr Phe Leu 
        195                 200                 205             


Tyr His Arg Pro Ala Ile Gly Val Ile Val Gly Cys Glu Leu Met Leu 
    210                 215                 220                 


Arg Phe Val Ala Val Gly Leu Ile Val Gly Thr Ala Phe Ile Ser Arg 
225                 230                 235                 240 


Gly Ala Cys Ala Ile Thr Tyr Pro Leu Phe Leu Thr Ile Thr Thr Trp 
                245                 250                 255     


Cys Phe Val Ser Thr Ile Gly Leu Thr Glu Leu Tyr Cys Ile Leu Arg 
            260                 265                 270         


Arg Gly Pro Ala Pro Lys Asn Ala Asp Lys Ala Ala Ala Pro Gly Arg 
        275                 280                 285             


Ser Lys Gly Leu Ser Gly Val Cys Gly Arg Cys Cys Ser Ile Ile Leu 
    290                 295                 300                 


Ser Gly Ile Ala Val Arg Leu Cys Tyr Ile Ala Val Val Ala Gly Val 
305                 310                 315                 320 


Val Leu Val Ala Leu His Tyr Glu Gln Glu Ile Gln Arg Arg Leu Phe 
                325                 330                 335     


Asp Val 
        


<210>  3
<211>  1017
<212>  DNA
<213>  herpes simplex virus 1

<400>  3
atgctcgccg tccgttccct gcagcacctc tcaaccgtcg tcttgataac ggcgtacggc       60

ctcgtgctcg tgtggtacac cgtcttcggt gccagtccgc tgcaccgatg tatttacgtg      120

gtacgcccca ccggcaccaa caacgacacc gccctcgtgt ggatgaaaat gaaccagacc      180

ctattgtttc tgggggcccc gacgcacccc cccaacgggg gctggcgcaa ccacgcccac      240

atctcgtacg ccaatctgat agcggggagg gtcgtgccct tccaggtccc acccgacgcc      300

atgaatcgtc ggatcatgaa cgtccacgag gccgtcaact gtctggagac cctatggtac      360

acacgggtgc gtctggtggt cgtagggtgg ttcctgtatc tggcgttcgt cgccctccac      420

caacgccgat gtatgtttgg cgtcgtgagt cccgcccaca agatggtggc cccggccacc      480

tacctcttga actacgcagg ccgcatcgta tcgagcgtgt tcctgcagta cccctacacg      540

aaaattaccc gcctgctctg cgagctgtcg gtccagcggc aaaacctggt tcagttgttt      600

gagacggacc cggtcacctt cttgtaccac cgccccgcca tcggggtcat cgtgggctgc      660

gagttgatcg tacgctttgt ggccgtgggt ctcatcgtcg gcaccgcttt catatcccgg      720

ggggcatgtg cgatcacata ccccctgttt ctgaccatca ccacctggtg ttttgtctcc      780

accatcggcc tgacagagct gtattgtatt ctgcggcggg gcccggcccc caagaacgca      840

gacaaggccg ccgccccggg gcgatccaag gggctgtcgg gcgtctgcgg gcgctgttgt      900

tccatcatcc tgtcgggcat cgcaatgcga ttgtgttata tcgccgtggt ggccggggtg      960

gtgctcgtgg cgcttcacta cgagcaggag atccagaggc gcctgtttga tgtatga        1017


<210>  4
<211>  338
<212>  PRT
<213>  herpes simplex virus 1

<400>  4

Met Leu Ala Val Arg Ser Leu Gln His Leu Ser Thr Val Val Leu Ile 
1               5                   10                  15      


Thr Ala Tyr Gly Leu Val Leu Val Trp Tyr Thr Val Phe Gly Ala Ser 
            20                  25                  30          


Pro Leu His Arg Cys Ile Tyr Val Val Arg Pro Thr Gly Thr Asn Asn 
        35                  40                  45              


Asp Thr Ala Leu Val Trp Met Lys Met Asn Gln Thr Leu Leu Phe Leu 
    50                  55                  60                  


Gly Ala Pro Thr His Pro Pro Asn Gly Gly Trp Arg Asn His Ala His 
65                  70                  75                  80  


Ile Ser Tyr Ala Asn Leu Ile Ala Gly Arg Val Val Pro Phe Gln Val 
                85                  90                  95      


Pro Pro Asp Ala Met Asn Arg Arg Ile Met Asn Val His Glu Ala Val 
            100                 105                 110         


Asn Cys Leu Glu Thr Leu Trp Tyr Thr Arg Val Arg Leu Val Val Val 
        115                 120                 125             


Gly Trp Phe Leu Tyr Leu Ala Phe Val Ala Leu His Gln Arg Arg Cys 
    130                 135                 140                 


Met Phe Gly Val Val Ser Pro Ala His Lys Met Val Ala Pro Ala Thr 
145                 150                 155                 160 


Tyr Leu Leu Asn Tyr Ala Gly Arg Ile Val Ser Ser Val Phe Leu Gln 
                165                 170                 175     


Tyr Pro Tyr Thr Lys Ile Thr Arg Leu Leu Cys Glu Leu Ser Val Gln 
            180                 185                 190         


Arg Gln Asn Leu Val Gln Leu Phe Glu Thr Asp Pro Val Thr Phe Leu 
        195                 200                 205             


Tyr His Arg Pro Ala Ile Gly Val Ile Val Gly Cys Glu Leu Ile Val 
    210                 215                 220                 


Arg Phe Val Ala Val Gly Leu Ile Val Gly Thr Ala Phe Ile Ser Arg 
225                 230                 235                 240 


Gly Ala Cys Ala Ile Thr Tyr Pro Leu Phe Leu Thr Ile Thr Thr Trp 
                245                 250                 255     


Cys Phe Val Ser Thr Ile Gly Leu Thr Glu Leu Tyr Cys Ile Leu Arg 
            260                 265                 270         


Arg Gly Pro Ala Pro Lys Asn Ala Asp Lys Ala Ala Ala Pro Gly Arg 
        275                 280                 285             


Ser Lys Gly Leu Ser Gly Val Cys Gly Arg Cys Cys Ser Ile Ile Leu 
    290                 295                 300                 


Ser Gly Ile Ala Met Arg Leu Cys Tyr Ile Ala Val Val Ala Gly Val 
305                 310                 315                 320 


Val Leu Val Ala Leu His Tyr Glu Gln Glu Ile Gln Arg Arg Leu Phe 
                325                 330                 335     


Asp Val 
        


<210>  5
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  F-primer

<400>  5
tcgaggagaa tcctggccca atgctcgccg tccgttccct g                           41


<210>  6
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R-primer

<400>  6
tccgatttaa attcgaattc tcatacatca aacaggcgcc tctg                        44


