                         SEQUENCE LISTING

<110>  Gundersen Lutheran Medical Foundation, Inc.
       Kenny, Paraic
       Lofgren, Kristopher
       Sreekumar, Sreeja
 
<120>  ANTIBODIES TARGETING AN AMPHIREGULIN-DERIVED CELL SURFACE 
       NEO-EPITOPE

<130>  67641.146

<150>  63/211,356
<151>  2021-06-16

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  252
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Arg Ala Pro Leu Leu Pro Pro Ala Pro Val Val Leu Ser Leu Leu 
1               5                   10                  15      


Ile Leu Gly Ser Gly His Tyr Ala Ala Gly Leu Asp Leu Asn Asp Thr 
            20                  25                  30          


Tyr Ser Gly Lys Arg Glu Pro Phe Ser Gly Asp His Ser Ala Asp Gly 
        35                  40                  45              


Phe Glu Val Thr Ser Arg Ser Glu Met Ser Ser Gly Ser Glu Ile Ser 
    50                  55                  60                  


Pro Val Ser Glu Met Pro Ser Ser Ser Glu Pro Ser Ser Gly Ala Asp 
65                  70                  75                  80  


Tyr Asp Tyr Ser Glu Glu Tyr Asp Asn Glu Pro Gln Ile Pro Gly Tyr 
                85                  90                  95      


Ile Val Asp Asp Ser Val Arg Val Glu Gln Val Val Lys Pro Pro Gln 
            100                 105                 110         


Asn Lys Thr Glu Ser Glu Asn Thr Ser Asp Lys Pro Lys Arg Lys Lys 
        115                 120                 125             


Lys Gly Gly Lys Asn Gly Lys Asn Arg Arg Asn Arg Lys Lys Lys Asn 
    130                 135                 140                 


Pro Cys Asn Ala Glu Phe Gln Asn Phe Cys Ile His Gly Glu Cys Lys 
145                 150                 155                 160 


Tyr Ile Glu His Leu Glu Ala Val Thr Cys Lys Cys Gln Gln Glu Tyr 
                165                 170                 175     


Phe Gly Glu Arg Cys Gly Glu Lys Ser Met Lys Thr His Ser Met Ile 
            180                 185                 190         


Asp Ser Ser Leu Ser Lys Ile Ala Leu Ala Ala Ile Ala Ala Phe Met 
        195                 200                 205             


Ser Ala Val Ile Leu Thr Ala Val Ala Val Ile Thr Val Gln Leu Arg 
    210                 215                 220                 


Arg Gln Tyr Val Arg Lys Tyr Glu Gly Glu Ala Glu Glu Arg Lys Lys 
225                 230                 235                 240 


Leu Arg Gln Glu Asn Gly Asn Val His Ala Ile Ala 
                245                 250         


<210>  2
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  2

Thr His Ser Met Ile Asp Ser Ser Leu Ser Lys Ile 
1               5                   10          


<210>  3
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1A3 antibody, Heavy chain

<400>  3

Gln Ser Val Lys Glu Ser Glu Gly Arg Leu Val Thr Pro Gly Thr Pro 
1               5                   10                  15      


Leu Arg Leu Thr Cys Thr Val Ala Gly Tyr Ser Leu Ser Arg Tyr His 
            20                  25                  30          


Met Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly 
        35                  40                  45              


Met Ile Gly Gly Ser Gly Arg Thr Asp Tyr Ala Ser Trp Ala Lys Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Lys Thr Ser Thr Thr Val Asp Leu Lys Ile Thr 
65                  70                  75                  80  


Ser Pro Thr Thr Glu Asp Thr Ala Thr Tyr Phe Cys Ser Thr Val Tyr 
                85                  90                  95      


Thr Asp Ser Asp Gly Asp Phe Trp Gly Pro Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  4
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1A3 antibody, Light chain

<400>  4

Ala Glu Leu Asp Met Thr Gln Thr Pro Ser Ser Lys Ser Val Pro Val 
1               5                   10                  15      


Gly Gly Thr Val Thr Ile Asn Cys Gln Ala Ser Glu Ser Val Tyr Ser 
            20                  25                  30          


Asn Asp Arg Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro Lys 
        35                  40                  45              


Leu Leu Ile Tyr Tyr Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg 
    50                  55                  60                  


Phe Lys Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu Thr Ile Ser Asp 
65                  70                  75                  80  


Leu Glu Cys Ala Asp Ala Ala Thr Tyr Tyr Cys Gln Ser Cys Tyr Asp 
                85                  90                  95      


Met Ser Ser Tyr Gly Val Ala Phe Gly Gly Gly Thr Glu Leu Glu Ile 
            100                 105                 110         


Leu 
    


<210>  5
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3A3 antibody, Heavy chain

<400>  5

Gln Gln Gln Leu Val Glu Ser Gly Gly Arg Leu Val Thr Pro Gly Thr 
1               5                   10                  15      


Pro Leu Thr Leu Thr Tyr Thr Val Ser Gly Phe Ser Leu Ser Asp Tyr 
            20                  25                  30          


His Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Gln Trp Ile 
        35                  40                  45              


Ala Thr Ile Ser Asn Arg Gly Asn Thr Tyr Tyr Ala Ser Trp Ala Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Lys Thr Ser Thr Thr Val Asp Leu Lys Ile 
65                  70                  75                  80  


Thr Ser Pro Thr Thr Glu Asp Thr Ala Thr Tyr Phe Cys Ala Arg Asp 
                85                  90                  95      


Asn Val His Gly Asp Gly Val Leu Val Phe Tyr Leu Trp Gly Pro Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  6
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3A3 antibody, Light chain

<400>  6

Ala Glu Leu Val Met Thr Gln Thr Pro Ala Ser Val Ser Glu Pro Val 
1               5                   10                  15      


Gly Gly Thr Val Thr Ile Lys Cys Gln Ala Ser Gln Ser Ile Gly Thr 
            20                  25                  30          


Asn Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Arg Leu 
        35                  40                  45              


Ile Tyr Lys Ala Ser Thr Leu Ala Ser Gly Val Ser Ser Arg Phe Lys 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Asp Leu Glu 
65                  70                  75                  80  


Cys Ala Asp Ala Ala Thr Tyr Tyr Cys Gln Gly Tyr Tyr Ser Gly Asp 
                85                  90                  95      


Ser Asn Ala Phe Gly Gly Gly Thr Glu Val Val Val Lys 
            100                 105                 


<210>  7
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3E4 antibody, Heavy chain

<400>  7

Gln Ser Val Lys Glu Ser Glu Gly Arg Leu Val Thr Pro Gly Thr Pro 
1               5                   10                  15      


Leu Thr Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Ser Asp Tyr Trp 
            20                  25                  30          


Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly 
        35                  40                  45              


Ile Ala Gly Tyr Ser Asp Asn Thr Tyr Tyr Ala Ser Arg Ala Lys Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Lys Thr Ser Thr Thr Val Val Leu Lys Ile Thr 
65                  70                  75                  80  


Arg Pro Thr Thr Glu Asp Thr Ala Thr Tyr Phe Cys Val Arg Asp Leu 
                85                  90                  95      


Tyr Gly Val Tyr Ser Ser Gly Thr Thr Leu Trp Gly Pro Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  8
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3E4 antibody, Light chain

<400>  8

Ala Glu Leu Val Met Thr Gln Thr Pro Ser Pro Val Ser Ala Ala Val 
1               5                   10                  15      


Gly Gly Thr Val Thr Ile Ser Cys Gln Ser Ser Gln Ser Val Tyr Asn 
            20                  25                  30          


Asn Asn Arg Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro Lys 
        35                  40                  45              


Leu Leu Ile Tyr Tyr Ala Ser Thr Leu Ala Ser Gly Val Ser Ser Arg 
    50                  55                  60                  


Phe Lys Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu Ile Ile Ser Asp 
65                  70                  75                  80  


Val Val Cys Asp Asp Ala Ala Thr Tyr Tyr Cys Gln Gly Tyr Tyr Ser 
                85                  90                  95      


Gly Gly Ile Thr Gly Phe Gly Gly Gly Thr Glu Val Val Val Lys 
            100                 105                 110     


<210>  9
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence representing uncleaved AREG, used for antibody 
       identification

<400>  9

Ser Met Lys Thr His Ser Met Ile Asp Ser Ser Leu Ser Lys Ile Ala 
1               5                   10                  15      


Cys 
    


