               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> IMPROVED BACILLUS HOST CELL 

<130> 34051-WO-PCT

<150>  EP21180004.0
<151>  2021-06-17

<160> 38

<170> BiSSAP 1.3.6

<210> 1
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW41

<400> 1
cagatcatcc ttaatcaggg gtagctaacg                                     30


<210> 2
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW42

<400> 2
gaaacttctc tcaattagat ttcatgtaaa ccgagaacca                           40


<210> 3
<211> 45
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW44

<400> 3
aactttttaa ctagaaagcg cacgtagtgt gattatatca ccgtg                     45


<210> 4
<211> 45
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW45

<400> 4
gcaaaagccc ttatgagggc ttttttaatt gttgtttgca gtaac                     45


<210> 5
<211> 49
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer B46

<400> 5
acccctgatt aaggatgatc tgatgttacc tcctataata ttttttccg                 49


<210> 6
<211> 2886
<212> DNA
<213> Artificial Sequence


<220> 
<223> toxin DNA fragment amplified from TMO310 genomic DNA with lac
      operator, inducible Pspac-masF and a spectinomycin resistance
      cassette

<400> 6
ctaattgaga gaagtttcta tagaattttt catatactta acgagtgctt tcacctttga      60

atatagtcct tcccacttat catcacactc tccccgatag ccttttctag ctatatccag     120

taaagttaca tgctctttag gtaaaagagg tatagcccat tctgcagcga catctttcga     180

ggtaatttca ccagtagtca ctgtttgcca cattcgagct agggttaaaa ttacattacg     240

ctcatcacct tttatcccct caattagttc tggcaaagaa tccttaattg ctcttcgaat     300

atctgtcaaa ggtacggaga caagtatact tgaagaatca ggaccaaata gagaaatact     360

attctttctt gcttgtgcta aaacaatagc caaatcagga tcatagcttg gttcctgaat     420

ttgtccattc tcaaattcac ccctgagcca ctcaccgtat ataaattctc tttttggagg     480

atattgccaa gggacaactt cactcctatt tataaccgta acttcaagtg gtctaacaga     540

atccgtattt ccaatctttc ctgatatagt cattagtctt tctgttagtt tttttcgagt     600

taattgaggt aaactatgat tcacgacgac tagaacatct acatcgctgt taatgcgtaa     660

accaccattt actgctgaac caaatagata tactccaact attgaacttc caaataaatc     720

ttttacgatt tttaatgttt gaatcgcttg atttggtatt tttccgttaa tcaaattgct     780

catgatttca cctcgttgat tatgttcata taaagtttat attgatactc aatttactta     840

ccctagattg gacatatact taaattactg ttcaataaag ctgaccgtta gcgtttaagt     900

acatcctttc acaatttgtc tacagattaa taattattct ttattataca gatctccata     960

taatttttga agcttgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc    1020

cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag    1080

ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc    1140

ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt    1200

gatggtggtt gacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac    1260

cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc    1320

catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat    1380

ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat    1440

ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact    1500

taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc    1560

cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac    1620

atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc    1680

atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc    1740

cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag    1800

ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact    1860

ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt    1920

gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac    1980

gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc    2040

gacatcgtat aacgttactg gtttcatcaa aatcgtctcc ctccgtttga atatttgatt    2100

gatcgtaacc agatgaagca ctctttccac tatccctaca gtgttatggc ttgaacaatc    2160

acgaaacaat aattggtacg tacgatcttt cagccgactc aaacatcaaa tcttacaaat    2220

gtagtctttg aaagtattac atatgtaaga tttaaatgca accgtttttt cggaaggaaa    2280

tgatgacctc gtttccaccg aattagcttg aaatagtaca taatggattt ccttacgcga    2340

aatacgggca gaatctctgc agtcggcgct cgcgacctct agagtcaaat gtgagcagta    2400

acaacctctg ctaaaattcc tgaaaaattt tgcaaaaagt tgttgacttt atctacaagg    2460

tgtggcataa tgtgtggaat tgtgagcgct cacaattgac ctgcaggcat gcctgcaaag    2520

cttgaattca ttaaagagga gaaattaact atggtaagcc gatacgtacc cgatatgggc    2580

gatctgattt gggttgattt tgacccgaca aaaggtagcg agcaagctgg acatcgtcca    2640

gctgttgtcc tgagtccttt catgtacaac aacaaaacag gtatgtgtct gtgtgttcct    2700

tgtacaacgc aatcaaaagg atatccgttc gaagttgttt tatccggtca ggaacgtgat    2760

ggcgtagcgt tagctgatca ggtaaaaagt atcgcctggc gggcaagagg agcaacgaag    2820

aaaggaacag ttgccccaga ggaattacaa ctcattaaag ccaaaattaa cgtactgatt    2880

gggtag                                                               2886


<210> 7
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW05

<400> 7
ctaattgaga gaagtttcta tagaatttt                                      29


<210> 8
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW06

<400> 8
ctacccaatc agtacgttaa ttt                                            23


<210> 9
<211> 465
<212> DNA
<213> Bacillus subtilis subsp. 168


<220> 
<223> BSU_00830 | ctsR

<400> 9
gtgggacata atatttctga catcattgaa caatatttaa aacgagtgtt agatcaaaat      60

ggcaaggaaa ttttagagat taaacgaagt gaaattgcag ataaatttca atgcgttcct     120

tcccaaataa attatgtcat caacaccaga tttacaagcg aaagaggata tattgttgag     180

agcaaacgcg ggggcggcgg ttacatcaga atcattaaaa ttaaaatgaa caatgaagtt     240

gtcctgatca ataatataat ttctcaaatt aatacccatt tgtctcaagc agcctctgat     300

gacattattt taaggctatt agaagacaag gtaatttcag aaagagaagc aaaaatgatg     360

gtcagcgtaa tggaccgctc agttttacac attgacttac ctgaacgtga tgaattaaga     420

gcgagaatga tgaaggcaat gctgacttct ttaaaattaa aataa                     465


<210> 10
<211> 154
<212> PRT
<213> Bacillus subtilis subsp. 168


<220> 
<223> BSU_00830 | CtsR

<400> 10
Met Gly His Asn Ile Ser Asp Ile Ile Glu Gln Tyr Leu Lys Arg Val 
1               5                   10                  15      
Leu Asp Gln Asn Gly Lys Glu Ile Leu Glu Ile Lys Arg Ser Glu Ile 
            20                  25                  30          
Ala Asp Lys Phe Gln Cys Val Pro Ser Gln Ile Asn Tyr Val Ile Asn 
        35                  40                  45              
Thr Arg Phe Thr Ser Glu Arg Gly Tyr Ile Val Glu Ser Lys Arg Gly 
    50                  55                  60                  
Gly Gly Gly Tyr Ile Arg Ile Ile Lys Ile Lys Met Asn Asn Glu Val 
65                  70                  75                  80  
Val Leu Ile Asn Asn Ile Ile Ser Gln Ile Asn Thr His Leu Ser Gln 
                85                  90                  95      
Ala Ala Ser Asp Asp Ile Ile Leu Arg Leu Leu Glu Asp Lys Val Ile 
            100                 105                 110         
Ser Glu Arg Glu Ala Lys Met Met Val Ser Val Met Asp Arg Ser Val 
        115                 120                 125             
Leu His Ile Asp Leu Pro Glu Arg Asp Glu Leu Arg Ala Arg Met Met 
    130                 135                 140                 
Lys Ala Met Leu Thr Ser Leu Lys Leu Lys 
145                 150                 

<210> 11
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer TS22

<400> 11
gacagatgac agcgaagttg at                                             22


<210> 12
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer TS23

<400> 12
accgaagtgg gcatccat                                                  18


<210> 13
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer ZT153

<400> 13
catgtctgga gacggagaaa ttatc                                          25


<210> 14
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer ZT155

<400> 14
gggcggacca gttacgatca g                                              21


<210> 15
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW_278

<400> 15
aggctgtcaa gcagtttttg                                                20


<210> 16
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW_279

<400> 16
agttgagccg tagaggtc                                                  18


<210> 17
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW274

<400> 17
cctttcctga cttttggaac                                                20


<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW275

<400> 18
atgttcaact cgattcgacg                                                20


<210> 19
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW 280

<400> 19
ggttcaaaag caatttaagt atgt                                           24


<210> 20
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW 281

<400> 20
gctttctaaa cgatcacgga                                                20


<210> 21
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW 276

<400> 21
gactgaaaca tcatcggcat                                                20


<210> 22
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW277

<400> 22
cagtggccga gcagtatt                                                  18


<210> 23
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW49

<400> 23
atgatcaatt gggggccgtt ttaacgattg ctgcc                                35


<210> 24
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW50

<400> 24
tcccgtctag ccttgccctc ttgacactcc ttatttga                             38


<210> 25
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW51

<400> 25
gggcaaggct agacgggact taccgaaaga aa                                   32


<210> 26
<211> 43
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW52

<400> 26
tatagaaact tctctcaatt agcccgctct tttggcaggc cgc                       43


<210> 27
<211> 37
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW53

<400> 27
aacgtactga ttgggtaggc cattcagaca tctccga                              37


<210> 28
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer BW54

<400> 28
cagacctggc attgatcgtg cctgtcagtt tac                                  33


<210> 29
<211> 2433
<212> DNA
<213> Bacillus subtilis subsp. 168


<220> 
<223> BSU_00860 | clpC gene

<400> 29
atgatgtttg gaagatttac agaacgagct caaaaagtac tggcgctagc acaggaagaa      60

gcacttcggt taggtcataa taacattggc actgagcata ttttattagg actggtaaga     120

gaaggagagg gcattgctgc taaagctctt caagcgcttg gactcggttc agaaaaaatt     180

cagaaagaag tagaaagttt gatcgggcgc gggcaggaaa tgtctcaaac gattcattat     240

actcctagag ctaaaaaagt cattgagctt tcaatggatg aggcaagaaa actcggtcat     300

tcttatgtgg gaacagaaca tattcttctt ggtctgattc gtgaaggaga aggtgttgct     360

gcgagagttc tgaataatct cggtgtcagc ttaaataaag caagacagca ggtgctccag     420

cttctaggaa gtaatgaaac aggatcatca gcggcaggaa caaacagcaa tgcgaatacg     480

cctacgcttg acagcttggc aagagactta actgctattg cgaaggaaga cagccttgac     540

cctgtaatcg gcagaagcaa ggagatccag cgtgtcattg aagtgttaag ccgcagaacg     600

aaaaacaacc ctgttctcat tggggaacca ggtgtaggta aaacggctat cgcagaaggt     660

ttggcacagc aaattatcaa taatgaagta cccgaaattt tgcgtgataa acgtgtgatg     720

acattagaca tgggaacagt tgttgccggc acaaaatacc gcggagaatt tgaggatcgc     780

ctgaagaagg tcatggatga aattcgccag gcaggaaata tcattctatt catcgatgag     840

ctccatacat taatcggggc aggcggagca gaaggtgcta ttgatgcatc taatatttta     900

aaaccttcac ttgctcgtgg cgaactccaa tgtattggtg caacgactct tgatgagtac     960

cgtaaatata ttgaaaaaga tgcagcactg gaacgccgtt ttcagccgat tcaggttgat    1020

cagccatctg tagatgaaag tattcaaatt ttacaaggtc tgcgtgacag atacgaagcc    1080

caccaccgcg tttctatcac tgatgatgcc attgaagctg cggttaagct ttctgacaga    1140

tatatttctg accgcttcct tccggataaa gcaattgact tgatcgatga agcgggttca    1200

aaggtgagac tgcgctcatt tacaacgcct cctaacttaa aagagcttga gcagaagctt    1260

gatgaggttc gtaaagagaa ggatgcggca gtgcaaagcc aagagtttga aaaagctgct    1320

tccttgcgtg atactgaaca acgcctgcgc gagcaagtag aggatacgaa gaaatcatgg    1380

aaagagaagc aagggcagga aaactcagag gttactgtgg atgatattgc gatggttgta    1440

tccagctgga ccggtgtgcc tgtatctaaa atcgcccaaa ctgaaactga taagcttctc    1500

aatatggaaa acattcttca ctcccgtgtc atcggccagg atgaagcagt tgtagcggtt    1560

gcaaaagccg tcagacgtgc aagagcagga ttgaaagatc ctaaacgccc aatcggctca    1620

ttcattttct taggccctac aggtgtaggt aaaacagaac ttgcacgagc acttgctgaa    1680

tccatttttg gcgatgaaga atccatgatc agaattgata tgtctgaata catggaaaaa    1740

cattcaactt caagacttgt tggttcacct ccgggttatg tgggatatga tgaaggcggt    1800

caattgacag agaaagtcag aagaaaacct tactctgtcg tgcttcttga tgagatcgag    1860

aaagcgcacc ctgatgtctt caatatcctt ctgcaagttc ttgaagacgg acgattgact    1920

gattctaaag gacgcacagt cgatttccgc aataccattc tgatcatgac atcaaacgtc    1980

ggagcaagtg agctaaaacg caataaatat gtcggcttta acgttcagga tgaaactcaa    2040

aatcataaag acatgaaaga taaagtgatg ggtgaattaa aacgagcgtt cagacctgag    2100

ttcatcaacc gtattgatga aatcattgtc ttccattcac ttgagaaaaa acatctcact    2160

gagattgtgt cattaatgtc tgatcaatta acgaaacgcc tgaaagaaca agatctttct    2220

atcgaattga cagatgctgc aaaagcgaaa gtcgcggaag agggcgttga cctggaatac    2280

ggtgcccgtc cgttaagaag agcgatccaa aaacatgtcg aggatcgttt atctgaagaa    2340

ctcctcagag gaaatattca taaaggacag catattgttc ttgatgtaga agatggcgaa    2400

tttgtcgtaa aaacgactgc taaaacgaat taa                                 2433


<210> 30
<211> 810
<212> PRT
<213> Bacillus subtilis subsp. 168


<220> 
<223> BSU_00860 | ClpC protein

<400> 30
Met Met Phe Gly Arg Phe Thr Glu Arg Ala Gln Lys Val Leu Ala Leu 
1               5                   10                  15      
Ala Gln Glu Glu Ala Leu Arg Leu Gly His Asn Asn Ile Gly Thr Glu 
            20                  25                  30          
His Ile Leu Leu Gly Leu Val Arg Glu Gly Glu Gly Ile Ala Ala Lys 
        35                  40                  45              
Ala Leu Gln Ala Leu Gly Leu Gly Ser Glu Lys Ile Gln Lys Glu Val 
    50                  55                  60                  
Glu Ser Leu Ile Gly Arg Gly Gln Glu Met Ser Gln Thr Ile His Tyr 
65                  70                  75                  80  
Thr Pro Arg Ala Lys Lys Val Ile Glu Leu Ser Met Asp Glu Ala Arg 
                85                  90                  95      
Lys Leu Gly His Ser Tyr Val Gly Thr Glu His Ile Leu Leu Gly Leu 
            100                 105                 110         
Ile Arg Glu Gly Glu Gly Val Ala Ala Arg Val Leu Asn Asn Leu Gly 
        115                 120                 125             
Val Ser Leu Asn Lys Ala Arg Gln Gln Val Leu Gln Leu Leu Gly Ser 
    130                 135                 140                 
Asn Glu Thr Gly Ser Ser Ala Ala Gly Thr Asn Ser Asn Ala Asn Thr 
145                 150                 155                 160 
Pro Thr Leu Asp Ser Leu Ala Arg Asp Leu Thr Ala Ile Ala Lys Glu 
                165                 170                 175     
Asp Ser Leu Asp Pro Val Ile Gly Arg Ser Lys Glu Ile Gln Arg Val 
            180                 185                 190         
Ile Glu Val Leu Ser Arg Arg Thr Lys Asn Asn Pro Val Leu Ile Gly 
        195                 200                 205             
Glu Pro Gly Val Gly Lys Thr Ala Ile Ala Glu Gly Leu Ala Gln Gln 
    210                 215                 220                 
Ile Ile Asn Asn Glu Val Pro Glu Ile Leu Arg Asp Lys Arg Val Met 
225                 230                 235                 240 
Thr Leu Asp Met Gly Thr Val Val Ala Gly Thr Lys Tyr Arg Gly Glu 
                245                 250                 255     
Phe Glu Asp Arg Leu Lys Lys Val Met Asp Glu Ile Arg Gln Ala Gly 
            260                 265                 270         
Asn Ile Ile Leu Phe Ile Asp Glu Leu His Thr Leu Ile Gly Ala Gly 
        275                 280                 285             
Gly Ala Glu Gly Ala Ile Asp Ala Ser Asn Ile Leu Lys Pro Ser Leu 
    290                 295                 300                 
Ala Arg Gly Glu Leu Gln Cys Ile Gly Ala Thr Thr Leu Asp Glu Tyr 
305                 310                 315                 320 
Arg Lys Tyr Ile Glu Lys Asp Ala Ala Leu Glu Arg Arg Phe Gln Pro 
                325                 330                 335     
Ile Gln Val Asp Gln Pro Ser Val Asp Glu Ser Ile Gln Ile Leu Gln 
            340                 345                 350         
Gly Leu Arg Asp Arg Tyr Glu Ala His His Arg Val Ser Ile Thr Asp 
        355                 360                 365             
Asp Ala Ile Glu Ala Ala Val Lys Leu Ser Asp Arg Tyr Ile Ser Asp 
    370                 375                 380                 
Arg Phe Leu Pro Asp Lys Ala Ile Asp Leu Ile Asp Glu Ala Gly Ser 
385                 390                 395                 400 
Lys Val Arg Leu Arg Ser Phe Thr Thr Pro Pro Asn Leu Lys Glu Leu 
                405                 410                 415     
Glu Gln Lys Leu Asp Glu Val Arg Lys Glu Lys Asp Ala Ala Val Gln 
            420                 425                 430         
Ser Gln Glu Phe Glu Lys Ala Ala Ser Leu Arg Asp Thr Glu Gln Arg 
        435                 440                 445             
Leu Arg Glu Gln Val Glu Asp Thr Lys Lys Ser Trp Lys Glu Lys Gln 
    450                 455                 460                 
Gly Gln Glu Asn Ser Glu Val Thr Val Asp Asp Ile Ala Met Val Val 
465                 470                 475                 480 
Ser Ser Trp Thr Gly Val Pro Val Ser Lys Ile Ala Gln Thr Glu Thr 
                485                 490                 495     
Asp Lys Leu Leu Asn Met Glu Asn Ile Leu His Ser Arg Val Ile Gly 
            500                 505                 510         
Gln Asp Glu Ala Val Val Ala Val Ala Lys Ala Val Arg Arg Ala Arg 
        515                 520                 525             
Ala Gly Leu Lys Asp Pro Lys Arg Pro Ile Gly Ser Phe Ile Phe Leu 
    530                 535                 540                 
Gly Pro Thr Gly Val Gly Lys Thr Glu Leu Ala Arg Ala Leu Ala Glu 
545                 550                 555                 560 
Ser Ile Phe Gly Asp Glu Glu Ser Met Ile Arg Ile Asp Met Ser Glu 
                565                 570                 575     
Tyr Met Glu Lys His Ser Thr Ser Arg Leu Val Gly Ser Pro Pro Gly 
            580                 585                 590         
Tyr Val Gly Tyr Asp Glu Gly Gly Gln Leu Thr Glu Lys Val Arg Arg 
        595                 600                 605             
Lys Pro Tyr Ser Val Val Leu Leu Asp Glu Ile Glu Lys Ala His Pro 
    610                 615                 620                 
Asp Val Phe Asn Ile Leu Leu Gln Val Leu Glu Asp Gly Arg Leu Thr 
625                 630                 635                 640 
Asp Ser Lys Gly Arg Thr Val Asp Phe Arg Asn Thr Ile Leu Ile Met 
                645                 650                 655     
Thr Ser Asn Val Gly Ala Ser Glu Leu Lys Arg Asn Lys Tyr Val Gly 
            660                 665                 670         
Phe Asn Val Gln Asp Glu Thr Gln Asn His Lys Asp Met Lys Asp Lys 
        675                 680                 685             
Val Met Gly Glu Leu Lys Arg Ala Phe Arg Pro Glu Phe Ile Asn Arg 
    690                 695                 700                 
Ile Asp Glu Ile Ile Val Phe His Ser Leu Glu Lys Lys His Leu Thr 
705                 710                 715                 720 
Glu Ile Val Ser Leu Met Ser Asp Gln Leu Thr Lys Arg Leu Lys Glu 
                725                 730                 735     
Gln Asp Leu Ser Ile Glu Leu Thr Asp Ala Ala Lys Ala Lys Val Ala 
            740                 745                 750         
Glu Glu Gly Val Asp Leu Glu Tyr Gly Ala Arg Pro Leu Arg Arg Ala 
        755                 760                 765             
Ile Gln Lys His Val Glu Asp Arg Leu Ser Glu Glu Leu Leu Arg Gly 
    770                 775                 780                 
Asn Ile His Lys Gly Gln His Ile Val Leu Asp Val Glu Asp Gly Glu 
785                 790                 795                 800 
Phe Val Val Lys Thr Thr Ala Lys Thr Asn 
                805                 810 

<210> 31
<211> 2100
<212> DNA
<213> Bacillus subtilis subsp. 168


<220> 
<223> BSU_13700 | clpE gene

<400> 31
atgcgttgtc aacattgtca tcaaaacgag gcgacgattc gccttaacat gcaaataaat      60

tccgttcata aacagatggt tctttgtgaa acttgctata acgaactgac ccgtaaacct     120

tcaatgagta tgggtcctca atctttcgga tttccgtttg aacaggcatt ccagccgaaa     180

gaacagagcg cagcaaaaca aagcgaaaaa aaagggttgc ttgatgagct ggctcaaaat     240

attacaaacg gtgcaaaagc cggtctcatt gatcccgtca tcggccgtga tgatgaagtg     300

gcgcgagtga tcgaaattct aaaccgccga aacaaaaaca atccggttct tattggtgag     360

ccgggtgtag ggaaaactgc catcgctgaa gggctcgctt taaaaattgc tgaaggtgat     420

gttccaaaca aactaaaaaa caaagagcta tacttgcttg atgttgcatc tcttgttgca     480

aacacaggga tcagaggcca atttgaggag agaatgaaac agctgatcac tgagctgaag     540

gaacgaaaaa atgtcattct gttcattgat gaaattcacc ttctcgtcgg cgcaggctct     600

gcagaaggat caatggacgc cggcaacatt ctcaaaccgg ccctagccag aggcgaactg     660

caagtcattg gcgcgacaac actgaaagaa tatcgtcaaa tcgaaaaaga tgccgcgctg     720

gaaagacgtt ttcagcctgt catggtgcag gagccttcaa ttgaacaggc tatcctcatt     780

ctgcaaggga ttaaagacaa atacgaggcg taccatggcg taacattcag tgatgaagca     840

atcaaagcat gtgtcacttt atcatcccgc tacattcagg acagacacct gccggataaa     900

gcaattgatt tattagatga agcaggttca aaagccaacc tgttaattga tgaactgaat     960

gatgaggatg ccgctgaacg cttaactgca attgaagccg aaaaaacaaa agccctggaa    1020

gaagaaaatt acgaactggc ggcaaaactc cgtgatgaag aactcgcatt ggagaaaaaa    1080

ctgaacagct cctccgctca taccgctgtc actgtggaag ctgagcacat tcaggaaatt    1140

gttgaacaaa aaacaggcat ccctgtcggc aaactgcagg cagacgaaca aacgaaaatg    1200

aaagaactcg aagcaaaact tcatgaacgc gtgatcggac aagaagccgc tgttcaaaaa    1260

gtggcaaaag cggtaagacg aagccgcgcc ggattaaaat ccaaaaacag accagtcggc    1320

tccttcctct tcgtcggtcc taccggcgta gggaaaacag agctttctaa aacactggca    1380

gatgaattat tcggcacaaa agacgctatt atccgactcg atatgagcga atacatggag    1440

aaacacgccg tatctaaaat tatcggttca ccgcctggat atgtcggcca tgaggaagct    1500

ggacaattaa ctgagaaggt gcgccgcaat ccttacagca ttgtgttgct ggatgagatt    1560

gaaaaagcac acccagacgt tcagcatatg ttcctgcaaa ttatggaaga tggccgtctg    1620

acagacagcc aaggcagaac tgtaagcttc aaagacactg tgatcatcat gacaagtaat    1680

gcgggtgctg gtgagaaaca aacgaaagtc ggtttccaat cagatgacag tgtcatcgaa    1740

gaacaaacat taattgattc actgagcatg ttctttaaac ctgagttcct caaccgtttt    1800

gacagcatta ttgagttccg ctcattggaa aaagaacatc ttgtcaaaat cgtcagcctt    1860

cttcttggag aacttgaaga aacattggcg gaacggggca ttagcttgaa tgtgacagat    1920

gaagcgaaag aaaaaatcgc tgagctgggc taccaccctt cattcggtgc acgtccgctt    1980

agaagaacca tccaagaatg ggttgaggat gaaatgaccg atctgctgct tgataatggc    2040

gagatcacaa gttttcacgt gattttagaa gatgataaaa tcaaagtgcg agcaaaataa    2100


<210> 32
<211> 699
<212> PRT
<213> Bacillus subtilis subsp. 168


<220> 
<223> BSU_13700 | ClpE protein

<400> 32
Met Arg Cys Gln His Cys His Gln Asn Glu Ala Thr Ile Arg Leu Asn 
1               5                   10                  15      
Met Gln Ile Asn Ser Val His Lys Gln Met Val Leu Cys Glu Thr Cys 
            20                  25                  30          
Tyr Asn Glu Leu Thr Arg Lys Pro Ser Met Ser Met Gly Pro Gln Ser 
        35                  40                  45              
Phe Gly Phe Pro Phe Glu Gln Ala Phe Gln Pro Lys Glu Gln Ser Ala 
    50                  55                  60                  
Ala Lys Gln Ser Glu Lys Lys Gly Leu Leu Asp Glu Leu Ala Gln Asn 
65                  70                  75                  80  
Ile Thr Asn Gly Ala Lys Ala Gly Leu Ile Asp Pro Val Ile Gly Arg 
                85                  90                  95      
Asp Asp Glu Val Ala Arg Val Ile Glu Ile Leu Asn Arg Arg Asn Lys 
            100                 105                 110         
Asn Asn Pro Val Leu Ile Gly Glu Pro Gly Val Gly Lys Thr Ala Ile 
        115                 120                 125             
Ala Glu Gly Leu Ala Leu Lys Ile Ala Glu Gly Asp Val Pro Asn Lys 
    130                 135                 140                 
Leu Lys Asn Lys Glu Leu Tyr Leu Leu Asp Val Ala Ser Leu Val Ala 
145                 150                 155                 160 
Asn Thr Gly Ile Arg Gly Gln Phe Glu Glu Arg Met Lys Gln Leu Ile 
                165                 170                 175     
Thr Glu Leu Lys Glu Arg Lys Asn Val Ile Leu Phe Ile Asp Glu Ile 
            180                 185                 190         
His Leu Leu Val Gly Ala Gly Ser Ala Glu Gly Ser Met Asp Ala Gly 
        195                 200                 205             
Asn Ile Leu Lys Pro Ala Leu Ala Arg Gly Glu Leu Gln Val Ile Gly 
    210                 215                 220                 
Ala Thr Thr Leu Lys Glu Tyr Arg Gln Ile Glu Lys Asp Ala Ala Leu 
225                 230                 235                 240 
Glu Arg Arg Phe Gln Pro Val Met Val Gln Glu Pro Ser Ile Glu Gln 
                245                 250                 255     
Ala Ile Leu Ile Leu Gln Gly Ile Lys Asp Lys Tyr Glu Ala Tyr His 
            260                 265                 270         
Gly Val Thr Phe Ser Asp Glu Ala Ile Lys Ala Cys Val Thr Leu Ser 
        275                 280                 285             
Ser Arg Tyr Ile Gln Asp Arg His Leu Pro Asp Lys Ala Ile Asp Leu 
    290                 295                 300                 
Leu Asp Glu Ala Gly Ser Lys Ala Asn Leu Leu Ile Asp Glu Leu Asn 
305                 310                 315                 320 
Asp Glu Asp Ala Ala Glu Arg Leu Thr Ala Ile Glu Ala Glu Lys Thr 
                325                 330                 335     
Lys Ala Leu Glu Glu Glu Asn Tyr Glu Leu Ala Ala Lys Leu Arg Asp 
            340                 345                 350         
Glu Glu Leu Ala Leu Glu Lys Lys Leu Asn Ser Ser Ser Ala His Thr 
        355                 360                 365             
Ala Val Thr Val Glu Ala Glu His Ile Gln Glu Ile Val Glu Gln Lys 
    370                 375                 380                 
Thr Gly Ile Pro Val Gly Lys Leu Gln Ala Asp Glu Gln Thr Lys Met 
385                 390                 395                 400 
Lys Glu Leu Glu Ala Lys Leu His Glu Arg Val Ile Gly Gln Glu Ala 
                405                 410                 415     
Ala Val Gln Lys Val Ala Lys Ala Val Arg Arg Ser Arg Ala Gly Leu 
            420                 425                 430         
Lys Ser Lys Asn Arg Pro Val Gly Ser Phe Leu Phe Val Gly Pro Thr 
        435                 440                 445             
Gly Val Gly Lys Thr Glu Leu Ser Lys Thr Leu Ala Asp Glu Leu Phe 
    450                 455                 460                 
Gly Thr Lys Asp Ala Ile Ile Arg Leu Asp Met Ser Glu Tyr Met Glu 
465                 470                 475                 480 
Lys His Ala Val Ser Lys Ile Ile Gly Ser Pro Pro Gly Tyr Val Gly 
                485                 490                 495     
His Glu Glu Ala Gly Gln Leu Thr Glu Lys Val Arg Arg Asn Pro Tyr 
            500                 505                 510         
Ser Ile Val Leu Leu Asp Glu Ile Glu Lys Ala His Pro Asp Val Gln 
        515                 520                 525             
His Met Phe Leu Gln Ile Met Glu Asp Gly Arg Leu Thr Asp Ser Gln 
    530                 535                 540                 
Gly Arg Thr Val Ser Phe Lys Asp Thr Val Ile Ile Met Thr Ser Asn 
545                 550                 555                 560 
Ala Gly Ala Gly Glu Lys Gln Thr Lys Val Gly Phe Gln Ser Asp Asp 
                565                 570                 575     
Ser Val Ile Glu Glu Gln Thr Leu Ile Asp Ser Leu Ser Met Phe Phe 
            580                 585                 590         
Lys Pro Glu Phe Leu Asn Arg Phe Asp Ser Ile Ile Glu Phe Arg Ser 
        595                 600                 605             
Leu Glu Lys Glu His Leu Val Lys Ile Val Ser Leu Leu Leu Gly Glu 
    610                 615                 620                 
Leu Glu Glu Thr Leu Ala Glu Arg Gly Ile Ser Leu Asn Val Thr Asp 
625                 630                 635                 640 
Glu Ala Lys Glu Lys Ile Ala Glu Leu Gly Tyr His Pro Ser Phe Gly 
                645                 650                 655     
Ala Arg Pro Leu Arg Arg Thr Ile Gln Glu Trp Val Glu Asp Glu Met 
            660                 665                 670         
Thr Asp Leu Leu Leu Asp Asn Gly Glu Ile Thr Ser Phe His Val Ile 
        675                 680                 685             
Leu Glu Asp Asp Lys Ile Lys Val Arg Ala Lys 
    690                 695                 

<210> 33
<211> 1263
<212> DNA
<213> Bacillus subtilis subsp. 168


<220> 
<223> BSU_28220 | clpX gene

<400> 33
atgtttaaat ttaacgagga aaaaggacaa ttaaaatgct cgttctgtgg aaaaacacaa      60

gatcaggttc gtaagcttgt agctggacct ggtgtatata tatgtgacga atgtatcgaa     120

ctctgcactg aaattgtaga ggaagaactc ggaacagaag aagaagtaga atttaaagac     180

gtaccaaagc ctcaggaaat tcgcgaaatt ttgaatgaat atgtcatcgg ccaagatcaa     240

gcgaagaaat cacttgctgt tgctgtgtat aaccactata agcgcattaa ctccaacagc     300

aaagttgatg atgttgagct ttcaaaaagt aatatttcat taatcggccc tacgggaagc     360

ggtaaaaccc ttctggcgca aacattggct cgcattttaa atgtgccgtt tgcgattgcg     420

gacgctacat ctttgactga agctggatac gtgggtgaag atgtagagaa tattctcttg     480

aagctcatcc aagctgctga ttatgatgtg gaaaaagccg aaaaaggcat tatctatatt     540

gatgaaatcg ataaagtggc tagaaaatct gaaaacccgt ctatcacacg tgatgtgtca     600

ggtgagggtg tacagcaggc attgcttaaa attcttgagg gtacagtggc aagcgtaccg     660

cctcaaggtg gacgtaagca tcctcatcaa gaattcattc aaattgacac aacaaatatt     720

ttgttcattt gcggcggagc tttcgatggt atcgaacaaa tcatcaaacg ccgtttaggc     780

caaaaagtga ttggattcgg tgccgacaat aaagctgctg atcttgagaa agaagatctt     840

ctttcaaaag tgcttccaga agatttgctc cgtttcgggt taattcctga atttatcgga     900

cgtcttccgg ttattgcaag ccttgaaaag cttgacgaag aagcattggt tgcgatctta     960

acaaaaccga aaaacgctct tgttaagcaa ttcaagaaaa tgcttgagct tgacaacgtt    1020

gagcttgagt ttgaagaaga agcgctttct gaaattgcta aaaaagcaat tgaacgcaag    1080

actggagcac gcggactccg ttctatcatt gaaggcatca tgcttgatgt gatgtttgag    1140

ctgccttctc gtgatgacat tgaaaaatgt gtaatcaccg gggcaactgt gacacacgga    1200

gagcctcctc gcctcttatt aaaagacggc actgaggtaa gccaagataa aacatctgca    1260

taa                                                                  1263


<210> 34
<211> 420
<212> PRT
<213> Bacillus subtilis subsp. 168


<220> 
<223> BSU_28220 | ClpX protein

<400> 34
Met Phe Lys Phe Asn Glu Glu Lys Gly Gln Leu Lys Cys Ser Phe Cys 
1               5                   10                  15      
Gly Lys Thr Gln Asp Gln Val Arg Lys Leu Val Ala Gly Pro Gly Val 
            20                  25                  30          
Tyr Ile Cys Asp Glu Cys Ile Glu Leu Cys Thr Glu Ile Val Glu Glu 
        35                  40                  45              
Glu Leu Gly Thr Glu Glu Glu Val Glu Phe Lys Asp Val Pro Lys Pro 
    50                  55                  60                  
Gln Glu Ile Arg Glu Ile Leu Asn Glu Tyr Val Ile Gly Gln Asp Gln 
65                  70                  75                  80  
Ala Lys Lys Ser Leu Ala Val Ala Val Tyr Asn His Tyr Lys Arg Ile 
                85                  90                  95      
Asn Ser Asn Ser Lys Val Asp Asp Val Glu Leu Ser Lys Ser Asn Ile 
            100                 105                 110         
Ser Leu Ile Gly Pro Thr Gly Ser Gly Lys Thr Leu Leu Ala Gln Thr 
        115                 120                 125             
Leu Ala Arg Ile Leu Asn Val Pro Phe Ala Ile Ala Asp Ala Thr Ser 
    130                 135                 140                 
Leu Thr Glu Ala Gly Tyr Val Gly Glu Asp Val Glu Asn Ile Leu Leu 
145                 150                 155                 160 
Lys Leu Ile Gln Ala Ala Asp Tyr Asp Val Glu Lys Ala Glu Lys Gly 
                165                 170                 175     
Ile Ile Tyr Ile Asp Glu Ile Asp Lys Val Ala Arg Lys Ser Glu Asn 
            180                 185                 190         
Pro Ser Ile Thr Arg Asp Val Ser Gly Glu Gly Val Gln Gln Ala Leu 
        195                 200                 205             
Leu Lys Ile Leu Glu Gly Thr Val Ala Ser Val Pro Pro Gln Gly Gly 
    210                 215                 220                 
Arg Lys His Pro His Gln Glu Phe Ile Gln Ile Asp Thr Thr Asn Ile 
225                 230                 235                 240 
Leu Phe Ile Cys Gly Gly Ala Phe Asp Gly Ile Glu Gln Ile Ile Lys 
                245                 250                 255     
Arg Arg Leu Gly Gln Lys Val Ile Gly Phe Gly Ala Asp Asn Lys Ala 
            260                 265                 270         
Ala Asp Leu Glu Lys Glu Asp Leu Leu Ser Lys Val Leu Pro Glu Asp 
        275                 280                 285             
Leu Leu Arg Phe Gly Leu Ile Pro Glu Phe Ile Gly Arg Leu Pro Val 
    290                 295                 300                 
Ile Ala Ser Leu Glu Lys Leu Asp Glu Glu Ala Leu Val Ala Ile Leu 
305                 310                 315                 320 
Thr Lys Pro Lys Asn Ala Leu Val Lys Gln Phe Lys Lys Met Leu Glu 
                325                 330                 335     
Leu Asp Asn Val Glu Leu Glu Phe Glu Glu Glu Ala Leu Ser Glu Ile 
            340                 345                 350         
Ala Lys Lys Ala Ile Glu Arg Lys Thr Gly Ala Arg Gly Leu Arg Ser 
        355                 360                 365             
Ile Ile Glu Gly Ile Met Leu Asp Val Met Phe Glu Leu Pro Ser Arg 
    370                 375                 380                 
Asp Asp Ile Glu Lys Cys Val Ile Thr Gly Ala Thr Val Thr His Gly 
385                 390                 395                 400 
Glu Pro Pro Arg Leu Leu Leu Lys Asp Gly Thr Glu Val Ser Gln Asp 
                405                 410                 415     
Lys Thr Ser Ala 
            420 

<210> 35
<211> 594
<212> DNA
<213> Bacillus subtilis subsp. 168


<220> 
<223> BSU_34540 | clpP gene

<400> 35
atgaatttaa tacctacagt cattgaacaa acgaaccgcg gggaaagagc gtatgacatt      60

tattctcgtc tattaaagga tcgtatcatc atgcttggat ctgcgattga tgacaacgtt     120

gcgaactcca tcgtgtcaca gcttttattc ttagcagcag aagaccctga aaaagaaatt     180

tctctttaca tcaacagccc gggcggctct attacagccg gtatggcgat ctatgatacc     240

atgcagttta ttaagccgaa ggtatctaca atttgtatcg gtatggctgc gtcaatgggc     300

gcgttcctgc ttgcagccgg cgaaaaaggc aaacgctatg cgcttccaaa cagtgaagtc     360

atgattcacc agcctcttgg cggtgcgcaa ggtcaagcga cagaaattga aattgctgcg     420

aaacgcattc tcttgcttcg cgacaaatta aacaaagtcc tagctgaacg tactggccag     480

ccgcttgaag tgatcgaacg cgacacagac cgtgataact tcaagtctgc tgaagaagcg     540

cttgaatacg gcctgattga caaaattttg actcacacag aagacaaaaa gtaa           594


<210> 36
<211> 197
<212> PRT
<213> Bacillus subtilis subsp. 168


<220> 
<223> BSU_34540 | ClpP protein

<400> 36
Met Asn Leu Ile Pro Thr Val Ile Glu Gln Thr Asn Arg Gly Glu Arg 
1               5                   10                  15      
Ala Tyr Asp Ile Tyr Ser Arg Leu Leu Lys Asp Arg Ile Ile Met Leu 
            20                  25                  30          
Gly Ser Ala Ile Asp Asp Asn Val Ala Asn Ser Ile Val Ser Gln Leu 
        35                  40                  45              
Leu Phe Leu Ala Ala Glu Asp Pro Glu Lys Glu Ile Ser Leu Tyr Ile 
    50                  55                  60                  
Asn Ser Pro Gly Gly Ser Ile Thr Ala Gly Met Ala Ile Tyr Asp Thr 
65                  70                  75                  80  
Met Gln Phe Ile Lys Pro Lys Val Ser Thr Ile Cys Ile Gly Met Ala 
                85                  90                  95      
Ala Ser Met Gly Ala Phe Leu Leu Ala Ala Gly Glu Lys Gly Lys Arg 
            100                 105                 110         
Tyr Ala Leu Pro Asn Ser Glu Val Met Ile His Gln Pro Leu Gly Gly 
        115                 120                 125             
Ala Gln Gly Gln Ala Thr Glu Ile Glu Ile Ala Ala Lys Arg Ile Leu 
    130                 135                 140                 
Leu Leu Arg Asp Lys Leu Asn Lys Val Leu Ala Glu Arg Thr Gly Gln 
145                 150                 155                 160 
Pro Leu Glu Val Ile Glu Arg Asp Thr Asp Arg Asp Asn Phe Lys Ser 
                165                 170                 175     
Ala Glu Glu Ala Leu Glu Tyr Gly Leu Ile Asp Lys Ile Leu Thr His 
            180                 185                 190         
Thr Glu Asp Lys Lys 
        195         

<210> 37
<211> 2325
<212> DNA
<213> Bacillus subtilis subsp. 168


<220> 
<223> BSU_28200 | lonA

<400> 37
atggcagaag aattaaaacg cagcatcccg ctcctccctt taagaggttt attagtgtac      60

ccgacgatgg ttttgcattt agatgtagga cgtgacaagt cggttcaggc tcttgaacag     120

gcaatgatgc atgatcatat gatattttta gccactcagc aggatatttc gatagacgaa     180

cctggtgaag acgagatttt cactgtcggc acctatacaa aaattaagca aatgctgaag     240

cttccgaacg gtacgattcg tgtgttggtg gagggcctca agcgcgccca tatcgtcaaa     300

tacaatgaac acgaggatta cacgtcggtt gacattcaac ttattcatga agatgattca     360

aaagatacag aagacgaagc attgatgcgg actttgctag accactttga tcagtacata     420

aaaatttcta aaaaaatctc ggccgaaacg tacgctgcgg ttactgatat cgaagagccg     480

gggagaatgg cagacatcgt tgcttctcat ctgcccctta aactaaagga taagcaggat     540

attttagaaa cggctgatgt caaagacagg ctgaacaagg tcattgattt tattaataat     600

gaaaaagaag tgctggagat tgagaaaaaa atcggccagc gcgtcaaacg ttcgatggag     660

cgtacgcaga aggaatacta cttgcgtgaa caaatgaagg cgatccaaaa agagcttggc     720

gataaagaag gcaaaactgg cgaagttcag acgctgacgg aaaaaatcga agaagcaggc     780

atgcctgatc atgtgaaaga aacagcgctt aaagaattga acagatatga aaaaattccg     840

tcaagttctg cggaaagctc tgtgatccgc aactatatcg actggctggt tgctcttccg     900

tggacggatg aaacagatga taagcttgat ttgaaagaag caggacgtct cttggacgaa     960

gagcaccacg ggcttgaaaa agtaaaagaa cgtattttag aatatttggc tgtccagaag    1020

ctgacaaaat ccctgaaagg cccgattctc tgtttggcag gacctccagg tgtcggaaaa    1080

acgtctttag ccaaatcaat tgcaaaaagc ttgggacgca aattcgtcag aatctcactc    1140

ggaggagttc gggatgaatc agagatacgc ggacacagac ggacctatgt cggagcaatg    1200

cctggacgta ttattcaagg gatgaaaaaa gcgggcaagc tgaatccggt cttcttatta    1260

gacgaaattg acaaaatgtc atctgatttt agaggagacc catcatccgc tatgcttgaa    1320

gtgcttgatc cagagcaaaa cagcagcttc agtgatcact atattgaaga aacctttgat    1380

ttatccaaag tgctgtttat cgcaacggcg aacaatttgg ccacaatccc gggcccgctc    1440

agagacagaa tggaaattat taatatcgca ggctacacag aaatagaaaa acttgaaatt    1500

gtaaaggatc acttgcttcc aaagcaaatc aaagaacacg ggctgaagaa aagcaatctt    1560

cagctgcgtg atcaggcgat tcttgatatt attcgttact atacgagaga agcgggtgta    1620

agaagccttg aacgccagct tgccgcaatt tgccgtaaag ctgcaaaagc cattgttgct    1680

gaagagcgaa aacgaattac ggttactgag aagaaccttc aagattttat cggaaaacgc    1740

attttcagat atggacaagc tgaaacagag gatcaagttg gtgtagtgac agggcttgcg    1800

tatacaaccg ttggcggaga tacgctttcg attgaagtat cgctttcacc gggaaaaggg    1860

aaattaatcc tgacaggaaa gctcggagat gttatgagag agtctgctca ggctgcattc    1920

agctatgtgc gatccaaaac agaagaactt ggcattgaac ctgactttca tgagaagtat    1980

gatattcata tacatgtacc agagggtgcg gtgccaaaag atggtccgtc agccggtatt    2040

acgatggcaa cagcgcttgt ttctgcttta acgggacggg cggtttcgcg tgaagtcggc    2100

atgactgggg aaatcacgct tcgcgggcgc gtgctgccga tcggcggctt aaaggaaaaa    2160

gcgcttggcg cacatagagc gggattaacg accattattg cgcctaaaga taatgaaaaa    2220

gatatagagg atattccgga aagcgtcagg gaggggctga catttatatt ggcgtcccac    2280

ttagacgagg ttttggaaca tgccttagta ggagagaaga aatga                    2325


<210> 38
<211> 774
<212> PRT
<213> Bacillus subtilis subsp. 168


<220> 
<223> BSU_ 28200 | LonA Protein sequence

<400> 38
Met Ala Glu Glu Leu Lys Arg Ser Ile Pro Leu Leu Pro Leu Arg Gly 
1               5                   10                  15      
Leu Leu Val Tyr Pro Thr Met Val Leu His Leu Asp Val Gly Arg Asp 
            20                  25                  30          
Lys Ser Val Gln Ala Leu Glu Gln Ala Met Met His Asp His Met Ile 
        35                  40                  45              
Phe Leu Ala Thr Gln Gln Asp Ile Ser Ile Asp Glu Pro Gly Glu Asp 
    50                  55                  60                  
Glu Ile Phe Thr Val Gly Thr Tyr Thr Lys Ile Lys Gln Met Leu Lys 
65                  70                  75                  80  
Leu Pro Asn Gly Thr Ile Arg Val Leu Val Glu Gly Leu Lys Arg Ala 
                85                  90                  95      
His Ile Val Lys Tyr Asn Glu His Glu Asp Tyr Thr Ser Val Asp Ile 
            100                 105                 110         
Gln Leu Ile His Glu Asp Asp Ser Lys Asp Thr Glu Asp Glu Ala Leu 
        115                 120                 125             
Met Arg Thr Leu Leu Asp His Phe Asp Gln Tyr Ile Lys Ile Ser Lys 
    130                 135                 140                 
Lys Ile Ser Ala Glu Thr Tyr Ala Ala Val Thr Asp Ile Glu Glu Pro 
145                 150                 155                 160 
Gly Arg Met Ala Asp Ile Val Ala Ser His Leu Pro Leu Lys Leu Lys 
                165                 170                 175     
Asp Lys Gln Asp Ile Leu Glu Thr Ala Asp Val Lys Asp Arg Leu Asn 
            180                 185                 190         
Lys Val Ile Asp Phe Ile Asn Asn Glu Lys Glu Val Leu Glu Ile Glu 
        195                 200                 205             
Lys Lys Ile Gly Gln Arg Val Lys Arg Ser Met Glu Arg Thr Gln Lys 
    210                 215                 220                 
Glu Tyr Tyr Leu Arg Glu Gln Met Lys Ala Ile Gln Lys Glu Leu Gly 
225                 230                 235                 240 
Asp Lys Glu Gly Lys Thr Gly Glu Val Gln Thr Leu Thr Glu Lys Ile 
                245                 250                 255     
Glu Glu Ala Gly Met Pro Asp His Val Lys Glu Thr Ala Leu Lys Glu 
            260                 265                 270         
Leu Asn Arg Tyr Glu Lys Ile Pro Ser Ser Ser Ala Glu Ser Ser Val 
        275                 280                 285             
Ile Arg Asn Tyr Ile Asp Trp Leu Val Ala Leu Pro Trp Thr Asp Glu 
    290                 295                 300                 
Thr Asp Asp Lys Leu Asp Leu Lys Glu Ala Gly Arg Leu Leu Asp Glu 
305                 310                 315                 320 
Glu His His Gly Leu Glu Lys Val Lys Glu Arg Ile Leu Glu Tyr Leu 
                325                 330                 335     
Ala Val Gln Lys Leu Thr Lys Ser Leu Lys Gly Pro Ile Leu Cys Leu 
            340                 345                 350         
Ala Gly Pro Pro Gly Val Gly Lys Thr Ser Leu Ala Lys Ser Ile Ala 
        355                 360                 365             
Lys Ser Leu Gly Arg Lys Phe Val Arg Ile Ser Leu Gly Gly Val Arg 
    370                 375                 380                 
Asp Glu Ser Glu Ile Arg Gly His Arg Arg Thr Tyr Val Gly Ala Met 
385                 390                 395                 400 
Pro Gly Arg Ile Ile Gln Gly Met Lys Lys Ala Gly Lys Leu Asn Pro 
                405                 410                 415     
Val Phe Leu Leu Asp Glu Ile Asp Lys Met Ser Ser Asp Phe Arg Gly 
            420                 425                 430         
Asp Pro Ser Ser Ala Met Leu Glu Val Leu Asp Pro Glu Gln Asn Ser 
        435                 440                 445             
Ser Phe Ser Asp His Tyr Ile Glu Glu Thr Phe Asp Leu Ser Lys Val 
    450                 455                 460                 
Leu Phe Ile Ala Thr Ala Asn Asn Leu Ala Thr Ile Pro Gly Pro Leu 
465                 470                 475                 480 
Arg Asp Arg Met Glu Ile Ile Asn Ile Ala Gly Tyr Thr Glu Ile Glu 
                485                 490                 495     
Lys Leu Glu Ile Val Lys Asp His Leu Leu Pro Lys Gln Ile Lys Glu 
            500                 505                 510         
His Gly Leu Lys Lys Ser Asn Leu Gln Leu Arg Asp Gln Ala Ile Leu 
        515                 520                 525             
Asp Ile Ile Arg Tyr Tyr Thr Arg Glu Ala Gly Val Arg Ser Leu Glu 
    530                 535                 540                 
Arg Gln Leu Ala Ala Ile Cys Arg Lys Ala Ala Lys Ala Ile Val Ala 
545                 550                 555                 560 
Glu Glu Arg Lys Arg Ile Thr Val Thr Glu Lys Asn Leu Gln Asp Phe 
                565                 570                 575     
Ile Gly Lys Arg Ile Phe Arg Tyr Gly Gln Ala Glu Thr Glu Asp Gln 
            580                 585                 590         
Val Gly Val Val Thr Gly Leu Ala Tyr Thr Thr Val Gly Gly Asp Thr 
        595                 600                 605             
Leu Ser Ile Glu Val Ser Leu Ser Pro Gly Lys Gly Lys Leu Ile Leu 
    610                 615                 620                 
Thr Gly Lys Leu Gly Asp Val Met Arg Glu Ser Ala Gln Ala Ala Phe 
625                 630                 635                 640 
Ser Tyr Val Arg Ser Lys Thr Glu Glu Leu Gly Ile Glu Pro Asp Phe 
                645                 650                 655     
His Glu Lys Tyr Asp Ile His Ile His Val Pro Glu Gly Ala Val Pro 
            660                 665                 670         
Lys Asp Gly Pro Ser Ala Gly Ile Thr Met Ala Thr Ala Leu Val Ser 
        675                 680                 685             
Ala Leu Thr Gly Arg Ala Val Ser Arg Glu Val Gly Met Thr Gly Glu 
    690                 695                 700                 
Ile Thr Leu Arg Gly Arg Val Leu Pro Ile Gly Gly Leu Lys Glu Lys 
705                 710                 715                 720 
Ala Leu Gly Ala His Arg Ala Gly Leu Thr Thr Ile Ile Ala Pro Lys 
                725                 730                 735     
Asp Asn Glu Lys Asp Ile Glu Asp Ile Pro Glu Ser Val Arg Glu Gly 
            740                 745                 750         
Leu Thr Phe Ile Leu Ala Ser His Leu Asp Glu Val Leu Glu His Ala 
        755                 760                 765             
Leu Val Gly Glu Lys Lys 
    770                 

