                         SEQUENCE LISTING

<110>  UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED
       RISE THERAPEUTICS, LLC
 
<120>  SURFACE LAYER PROTEIN A (SLPA) AS A THERAPEUTIC AGENT FOR THE 
       TREATMENT OF INFLAMMATORY DISEASES

<130>  UF.1357XC1PCT

<150>  US 63/209,426
<151>  2021-06-11

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer 132

<400>  1
gaccgccttt ccgttattat cc                                                22


<210>  2
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer 133

<400>  2
tggggcggaa gtggaaatac ttatgg                                            26


<210>  3
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer 123

<400>  3
gcatgctcga gtctagaagt cttataacta tac                                    33


<210>  4
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer 126

<400>  4
gtcgaccggt ttatcgaaaa ttagctactt ttac                                   34


<210>  5
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant SlpA

<400>  5

Met Gln Ser Ser Leu Lys Lys Ser Leu Tyr Leu Gly Leu Ala Ala Leu 
1               5                   10                  15      


Ser Phe Ala Gly Val Ala Ala Val Ser Thr Thr Ala Ser Ala Lys Ser 
            20                  25                  30          


Arg Met Ala Thr Thr Ile Asn Ala Ser Ser Ser Ala Ile Asn Thr Asn 
        35                  40                  45              


Thr Asn Ala Lys Tyr Asp Val Asp Val Thr Pro Ser Val Ser Ala Val 
    50                  55                  60                  


Ala Ala Asn Thr Ala Asn Asn Thr Pro Ala Ile Ala Gly Asn Leu Thr 
65                  70                  75                  80  


Gly Thr Ile Ser Ala Ser Tyr Asn Gly Lys Thr Tyr Thr Ala Asn Leu 
                85                  90                  95      


Lys Ala Asp Thr Glu Asn Ala Thr Ile Thr Ala Ala Gly Ser Thr Thr 
            100                 105                 110         


Ala Val Lys Pro Ala Glu Leu Ala Ala Gly Val Ala Tyr Thr Val Thr 
        115                 120                 125             


Val Asn Asp Val Ser Phe Asn Phe Gly Ser Glu Asn Ala Gly Lys Thr 
    130                 135                 140                 


Val Thr Leu Gly Ser Ala Asn Ser Asn Val Lys Phe Thr Gly Thr Asn 
145                 150                 155                 160 


Ser Asp Asn Gln Thr Glu Thr Asn Val Ser Thr Leu Lys Val Lys Leu 
                165                 170                 175     


Asp Gln Asn Gly Val Ala Ser Leu Thr Asn Val Ser Ile Ala Asn Val 
            180                 185                 190         


Tyr Ala Ile Asn Thr Thr Asp Asn Ser Asn Val Asn Phe Tyr Asp Val 
        195                 200                 205             


Thr Ser Gly Ala Thr Val Thr Asn Gly Ala Val Ser Val Asn Ala Asp 
    210                 215                 220                 


Asn Gln Gly Gln Val Asn Val Ala Asn Val Val Ala Ala Ile Asn Ser 
225                 230                 235                 240 


Lys Tyr Phe Ala Ala Gln Tyr Ala Asp Lys Lys Leu Asn Thr Arg Thr 
                245                 250                 255     


Ala Asn Thr Glu Asp Ala Ile Lys Ala Ala Leu Lys Asp Gln Lys Ile 
            260                 265                 270         


Asp Val Asn Ser Val Gly Tyr Phe Lys Ala Pro His Thr Phe Thr Val 
        275                 280                 285             


Asn Val Lys Ala Thr Ser Asn Thr Asn Gly Lys Ser Ala Thr Leu Pro 
    290                 295                 300                 


Val Val Val Thr Val Pro Asn Val Ala Glu Pro Thr Val Ala Ser Val 
305                 310                 315                 320 


Ser Lys Arg Ile Met His Asn Ala Tyr Tyr Tyr Asp Lys Asp Ala Lys 
                325                 330                 335     


Arg Val Gly Thr Asp Ser Val Lys Arg Tyr Asn Ser Val Ser Val Leu 
            340                 345                 350         


Pro Asn Thr Thr Thr Ile Asn Gly Lys Thr Tyr Tyr Gln Val Val Glu 
        355                 360                 365             


Asn Gly Lys Ala Val Asp Lys Tyr Ile Asn Ala Ala Asn Ile Asp Gly 
    370                 375                 380                 


Thr Lys Arg Thr Leu Lys His Asn Ala Tyr Val Tyr Ala Ser Ser Lys 
385                 390                 395                 400 


Lys Arg Ala Asn Lys Val Val Leu Lys Lys Gly Glu Val Val Thr Thr 
                405                 410                 415     


Tyr Gly Ala Ser Tyr Thr Phe Lys Asn Gly Gln Lys Tyr Tyr Lys Ile 
            420                 425                 430         


Gly Asp Asn Thr Asp Lys Thr Tyr Val Lys Val Ala Asn Phe Arg 
        435                 440                 445         


<210>  6
<211>  1344
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant slpA nucleotide sequence encoding SEQ ID NO: 5

<400>  6
atgcaatcaa gtttaaagaa atctctttac ttgggccttg ccgcattgag ctttgctggt       60

gttgctgccg tttcaacgac tgcttcagct aagtcgcgaa tggcgacaac tattaacgcg      120

tcttcatctg ctattaatac gaacacaaat gctaaatatg acgttgatgt caccccttct      180

gtgagtgctg tcgcagcaaa caccgctaat aatactcctg ccattgctgg taatttaaca      240

ggaacaatct cagcgagtta caatggtaaa acttatacag cgaaccttaa ggccgacact      300

gaaaatgcaa ccataactgc cgccggatca acaacagccg tcaaaccagc cgaattagca      360

gcaggtgttg cctatactgt cacagttaat gacgtttctt ttaattttgg ttctgaaaat      420

gctggtaaaa ctgtcacgtt gggatctgcc aattcaaatg tcaaattcac agggacaaat      480

tcagataacc aaaccgaaac taatgttagt acattgaaag ttaaattaga tcagaacgga      540

gtcgcatctt taacaaacgt ttctattgct aatgtctatg ctattaacac aacggacaac      600

tctaatgtaa acttttatga tgttacctca ggcgcaacag taactaatgg agcagtatct      660

gtcaacgccg ataatcaagg acaagttaat gttgccaacg tcgtagcagc tattaattct      720

aaatattttg ccgctcaata tgcagacaaa aaattgaata cacgaaccgc aaataccgaa      780

gatgcaatca aagccgccct taaagaccaa aaaatcgatg taaactcagt aggttatttt      840

aaagcacctc atacctttac agttaatgtt aaagccacat caaataccaa tggaaaatct      900

gctacattgc ctgttgtagt tactgtgcca aatgttgccg aacctacagt tgcctcagtg      960

tcaaaacgaa tcatgcataa tgcctactat tatgataaag atgctaaacg tgtaggaact     1020

gattcagtaa aaagatataa ctctgtaagt gtacttccaa acacaacaac aattaatgga     1080

aaaacgtatt atcaagtagt tgaaaatggg aaagcggtgg ataaatacat taatgcggca     1140

aatattgacg gtacgaaaag aacattaaaa cataatgcat atgtgtatgc ctctagtaaa     1200

aaacgagcta ataaagtagt cttaaaaaaa ggtgaagttg taacaactta tggggcctct     1260

tatactttca aaaatggtca aaaatattat aaaattggag ataatacaga taagacatat     1320

gtaaaagtag ctaattttcg ataa                                            1344


<210>  7
<211>  288
<212>  PRT
<213>  Lactobacillus acidophilus

<400>  7

Ala Ala Thr Thr Ile Asn Ala Ser Ser Ser Ala Ile Asn Thr Asn Thr 
1               5                   10                  15      


Asn Ala Lys Tyr Asp Val Asp Val Thr Pro Ser Val Ser Ala Val Ala 
            20                  25                  30          


Ala Val Ala Ala Asn Thr Ala Asn Asn Thr Pro Ala Ile Ala Gly Asn 
        35                  40                  45              


Leu Thr Gly Thr Ile Ser Ala Ser Tyr Asn Gly Lys Thr Tyr Thr Ala 
    50                  55                  60                  


Asn Leu Lys Ala Asp Thr Glu Asn Ala Thr Ile Thr Ala Ala Gly Ser 
65                  70                  75                  80  


Thr Thr Ala Val Lys Pro Ala Glu Leu Ala Ala Gly Val Ala Tyr Thr 
                85                  90                  95      


Val Thr Val Asn Asp Val Ser Phe Asn Phe Gly Ser Glu Asn Ala Gly 
            100                 105                 110         


Lys Thr Val Thr Leu Gly Ser Ala Asn Ser Asn Val Lys Phe Thr Gly 
        115                 120                 125             


Thr Asn Ser Asp Asn Gln Thr Glu Thr Asn Val Ser Thr Leu Lys Val 
    130                 135                 140                 


Lys Leu Asp Gln Asn Gly Val Ala Ser Leu Thr Asn Val Ser Ile Ala 
145                 150                 155                 160 


Asn Val Tyr Ala Ile Asn Thr Thr Asp Asn Ser Asn Val Asn Phe Tyr 
                165                 170                 175     


Asp Val Thr Ser Gly Ala Thr Val Thr Asn Gly Ala Val Ser Val Asn 
            180                 185                 190         


Ala Asp Asn Gln Gly Gln Val Asn Val Ala Asn Val Val Ala Ala Ile 
        195                 200                 205             


Asn Ser Lys Tyr Phe Ala Ala Gln Tyr Ala Asp Lys Lys Leu Asn Thr 
    210                 215                 220                 


Arg Thr Ala Asn Thr Glu Asp Ala Ile Lys Ala Ala Leu Lys Asp Gln 
225                 230                 235                 240 


Lys Ile Asp Val Asn Ser Val Gly Tyr Phe Lys Ala Pro His Thr Phe 
                245                 250                 255     


Thr Val Asn Val Lys Ala Thr Ser Asn Thr Asn Gly Lys Ser Ala Thr 
            260                 265                 270         


Leu Pro Val Val Val Thr Val Pro Asn Val Ala Glu Pro Thr Val Ala 
        275                 280                 285             


<210>  8
<211>  444
<212>  PRT
<213>  Lactobacillus acidophilus

<400>  8

Met Lys Lys Asn Leu Arg Ile Val Ser Ala Ala Ala Ala Ala Leu Leu 
1               5                   10                  15      


Ala Val Ala Pro Val Ala Ala Ser Ala Val Ser Thr Val Ser Ala Ala 
            20                  25                  30          


Thr Thr Ile Asn Ala Ser Ser Ser Ala Ile Asn Thr Asn Thr Asn Ala 
        35                  40                  45              


Lys Tyr Asp Val Asp Val Thr Pro Ser Val Ser Ala Val Ala Ala Asn 
    50                  55                  60                  


Thr Ala Asn Asn Thr Pro Ala Ile Ala Gly Asn Leu Thr Gly Thr Ile 
65                  70                  75                  80  


Ser Ala Ser Tyr Asn Gly Lys Thr Tyr Thr Ala Asn Leu Lys Ala Asp 
                85                  90                  95      


Thr Glu Asn Ala Thr Ile Thr Ala Ala Gly Ser Thr Thr Ala Val Lys 
            100                 105                 110         


Pro Ala Glu Leu Ala Ala Gly Val Ala Tyr Thr Val Thr Val Asn Asp 
        115                 120                 125             


Val Ser Phe Asn Phe Gly Ser Glu Asn Ala Gly Lys Thr Val Thr Leu 
    130                 135                 140                 


Gly Ser Ala Asn Ser Asn Val Lys Phe Thr Gly Thr Asn Ser Asp Asn 
145                 150                 155                 160 


Gln Thr Glu Thr Asn Val Ser Thr Leu Lys Val Lys Leu Asp Gln Asn 
                165                 170                 175     


Gly Val Ala Ser Leu Thr Asn Val Ser Ile Ala Asn Val Tyr Ala Ile 
            180                 185                 190         


Asn Thr Thr Asp Asn Ser Asn Val Asn Phe Tyr Asp Val Thr Ser Gly 
        195                 200                 205             


Ala Thr Val Thr Asn Gly Ala Val Ser Val Asn Ala Asp Asn Gln Gly 
    210                 215                 220                 


Gln Val Asn Val Ala Asn Val Val Ala Ala Ile Asn Ser Lys Tyr Phe 
225                 230                 235                 240 


Ala Ala Gln Tyr Ala Asp Lys Lys Leu Asn Thr Arg Thr Ala Asn Thr 
                245                 250                 255     


Glu Asp Ala Ile Lys Ala Ala Leu Lys Asp Gln Lys Ile Asp Val Asn 
            260                 265                 270         


Ser Val Gly Tyr Phe Lys Ala Pro His Thr Phe Thr Val Asn Val Lys 
        275                 280                 285             


Ala Thr Ser Asn Thr Asn Gly Lys Ser Ala Thr Leu Pro Val Val Val 
    290                 295                 300                 


Thr Val Pro Asn Val Ala Glu Pro Thr Val Ala Ser Val Ser Lys Arg 
305                 310                 315                 320 


Ile Met His Asn Ala Tyr Tyr Tyr Asp Lys Asp Ala Lys Arg Val Gly 
                325                 330                 335     


Thr Asp Ser Val Lys Arg Tyr Asn Ser Val Ser Val Leu Pro Asn Thr 
            340                 345                 350         


Thr Thr Ile Asn Gly Lys Thr Tyr Tyr Gln Val Val Glu Asn Gly Lys 
        355                 360                 365             


Ala Val Asp Lys Tyr Ile Asn Ala Ala Asn Ile Asp Gly Thr Lys Arg 
    370                 375                 380                 


Thr Leu Lys His Asn Ala Tyr Val Tyr Ala Ser Ser Lys Lys Arg Ala 
385                 390                 395                 400 


Asn Lys Val Val Leu Lys Lys Gly Glu Val Val Thr Thr Tyr Gly Ala 
                405                 410                 415     


Ser Tyr Thr Phe Lys Asn Gly Gln Lys Tyr Tyr Lys Ile Gly Asp Asn 
            420                 425                 430         


Thr Asp Lys Thr Tyr Val Lys Val Ala Asn Phe Arg 
        435                 440                 


<210>  9
<211>  469
<212>  PRT
<213>  Levilactobacillus brevis

<400>  9

Met Phe Gly Gly Arg Lys Ile Met Gln Ser Ser Leu Lys Lys Ser Leu 
1               5                   10                  15      


Tyr Leu Gly Leu Ala Ala Leu Ser Phe Ala Gly Val Ala Ala Val Ser 
            20                  25                  30          


Thr Thr Ala Ser Ala Lys Ser Tyr Ala Thr Ala Gly Ala Tyr Thr Thr 
        35                  40                  45              


Leu Lys Thr Asp Ala Thr Lys Arg Asn Val Glu Ala Thr Gly Thr Asn 
    50                  55                  60                  


Ala Leu Tyr Thr Lys Pro Gly Thr Val Lys Gly Ala Lys Val Val Ala 
65                  70                  75                  80  


Ser Lys Ala Thr Met Ala Lys Leu Ala Ser Ser Lys Lys Ser Ala Asp 
                85                  90                  95      


Tyr Phe Arg Ala Tyr Gly Val Lys Thr Thr Asn Arg Gly Ser Val Tyr 
            100                 105                 110         


Tyr Arg Val Val Ser Met Asp Gly Lys Tyr Arg Gly Tyr Val Tyr Gly 
        115                 120                 125             


Gly Lys Ser Asp Thr Ala Phe Ala Gly Gly Ile Lys Ser Ala Asp Thr 
    130                 135                 140                 


Thr Thr Thr Ala Thr Thr Pro Thr Arg Thr Thr Gly Tyr Tyr Leu Lys 
145                 150                 155                 160 


Asp Val Ser Lys Asn Thr Leu Trp Thr Ala Pro Lys Asn Thr Gln Tyr 
                165                 170                 175     


Lys Ala Ser Lys Val Ser Leu Tyr Gly Val Lys Ser Thr Asp Thr Phe 
            180                 185                 190         


Lys Val Asp Ser Ala Ala Thr Lys Thr Arg Glu Gly Ser Leu Tyr Tyr 
        195                 200                 205             


His Val Thr Asp Thr Gln Asn Thr Ser Val Ser Gly Trp Ile Tyr Ala 
    210                 215                 220                 


Gly Lys Gly Tyr Val Ala Gly Ala Thr Thr Gln Asp Leu Gly Gly Leu 
225                 230                 235                 240 


Ser Leu Thr Met Ser Asp Ala Ala Ala Thr Ser Asp Asn Ser Val Lys 
                245                 250                 255     


Val Val Tyr Arg Ala Ser Gly Ser Gln Val Gly Thr Ala Thr Trp Val 
            260                 265                 270         


Thr Ala Ala Ala Gly Thr Lys Ala Gly Ala Thr Val Gly Thr Thr Ala 
        275                 280                 285             


Val Asn Ala Ala Gly Val Lys Leu Ala Asp Phe Val Thr Asn Ser Leu 
    290                 295                 300                 


Pro Ser Gly Tyr Thr Thr Thr Gly Thr Val Asp Thr Ala Ser Ala Thr 
305                 310                 315                 320 


Tyr Gly Asn Thr Val Tyr Val Asp Val Thr Ala Ala Ala Thr Ser Lys 
                325                 330                 335     


Val Gln Leu Val Ala Asp Asn Val Asp Asn Thr Ala Ser Thr Thr Asp 
            340                 345                 350         


Asn Ala Val Ala Gly Val Leu Ala Asn Gly Ala Lys Leu Ser Ser Ser 
        355                 360                 365             


Asp Leu Ser Ala Thr Leu Lys Glu Ala Gly Ile Lys Ala Leu Thr Gly 
    370                 375                 380                 


Thr Lys Gly Glu Ala Ile Gly Ala Thr Asn Leu Ala Thr Ile Ser Gly 
385                 390                 395                 400 


Ala Phe Asp Thr Ala Glu Ile Asn Gly Ser Lys Thr Tyr Tyr Ala Ala 
                405                 410                 415     


Asn Gly Asp Ala Tyr His Tyr Val Phe Thr Tyr Glu Pro Ala Asn Phe 
            420                 425                 430         


Ala Asn Asp Asn Arg Leu Ala Thr Tyr Gly Asp Thr Leu Thr Ala Ser 
        435                 440                 445             


Phe Lys Ala Val Leu Thr Lys Gly Ala Pro Ser Ala Ser Ser Ser Asn 
    450                 455                 460                 


Ser Ser Trp Ile Ala 
465                 


<210>  10
<211>  451
<212>  PRT
<213>  Lactobacillus crispatus

<400>  10

Met Lys Lys Asn Leu Arg Ile Val Ser Ala Ala Ala Ala Ala Leu Leu 
1               5                   10                  15      


Ala Val Ala Pro Val Ala Ala Ser Ala Val Ser Val Asn Ala Ala Asp 
            20                  25                  30          


Asn Thr Val Ala Thr Thr Thr Asn Thr Ala Asn Thr Val Ile Asn Ala 
        35                  40                  45              


Asp Gly Thr Ala Ile Asn Thr Pro Ala Asp Ala Lys Tyr Asp Val Asp 
    50                  55                  60                  


Val Thr Pro Asn Leu Thr Ala Thr Ala Ala Ser Thr Val Asn Gly Gln 
65                  70                  75                  80  


Thr Ile Asn Gly Ser Ile Thr Gly Asn Ile Thr Ala Ser Tyr Asn Gly 
                85                  90                  95      


Gln Ser Tyr Thr Gly Thr Leu Asp Thr Lys Asn Gly Lys Val Ser Val 
            100                 105                 110         


Ala Asp Ser Lys Gly Thr Ala Val Thr Asp Phe Ser Lys Leu Thr Asn 
        115                 120                 125             


Gly Ser Tyr Thr Val Thr Val Ser Gly Val Ser Phe Asn Phe Gly Thr 
    130                 135                 140                 


Ala Asn Ala Asn Lys Thr Ile Thr Leu Gly Ser Lys Asn Ser Asn Val 
145                 150                 155                 160 


Lys Phe Ala Gly Ala Asp Gly Lys Phe Ala Asp Thr Val Lys Val Glu 
                165                 170                 175     


Leu Gly Gln Asn Gly Thr Leu Thr Thr Pro Ile Ser Val Gln Val Ser 
            180                 185                 190         


Asn Val Asn Ala Leu Asp Leu Ser Asn Ala Asn Gly Val Asn Phe Tyr 
        195                 200                 205             


Asn Ala Ser Asn Gly Ser Gln Val Thr Lys Gly Ser Val Asn Val Thr 
    210                 215                 220                 


Ala Gly Leu Ile Gly Arg Leu Asn Val Ser Thr Val Ala Ser Glu Ile 
225                 230                 235                 240 


Leu Lys Asn Cys Ala Ala Tyr Gln Val Ser Asn Gly Lys Pro Val Ser 
                245                 250                 255     


Gln Leu Pro Asp Gln Lys Ala Val Val Ala Asp Val Asn Ala Ala Leu 
            260                 265                 270         


Lys Ala Ala Asn Ile Pro Val Asp Asn Ala Gly Trp Phe Thr Ala Pro 
        275                 280                 285             


Ile Ser Leu Ser Val Asn Val Lys Ala Ser Ser Ser Ile Asn Gly Val 
    290                 295                 300                 


Gly Cys Tyr Phe Thr Cys Thr Val Asn Val Ala Asn Gly Lys Asp Met 
305                 310                 315                 320 


Thr Val Pro Ser Gln Ser Lys Thr Ile Met His Asn Ala Tyr Tyr Tyr 
                325                 330                 335     


Asp Lys Asp Ala Lys Arg Val Gly Thr Asp Lys Leu Thr Arg Tyr Asn 
            340                 345                 350         


Ser Val Thr Val Ala Met Asn Thr Thr Thr Ile Asn Gly Lys Ala Tyr 
        355                 360                 365             


Tyr Glu Val Ile Glu Asn Gly Lys Ala Thr Gly Lys Phe Ile Asn Ala 
    370                 375                 380                 


Asp Asn Ile Asp Gly Thr Lys Arg Thr Leu Lys His Asn Ala Tyr Val 
385                 390                 395                 400 


Tyr Lys Thr Ser Lys Lys Arg Ala Asn Lys Val Thr Leu Lys Lys Gly 
                405                 410                 415     


Thr Glu Val Thr Thr Tyr Gly Gly Thr Tyr Thr Phe Lys Asn Gly Lys 
            420                 425                 430         


Gln Tyr Tyr Lys Ile Gly Asn Asn Thr Asp Lys Thr Tyr Val Lys Ala 
        435                 440                 445             


Ser Asn Phe 
    450     


<210>  11
<211>  439
<212>  PRT
<213>  Lactobacillus crispatus

<400>  11

Met Lys Lys Asn Leu Arg Ile Val Ser Ala Ala Ala Ala Ala Leu Leu 
1               5                   10                  15      


Ala Val Ala Pro Val Ala Ala Ala Gly Val Ser Ser Val Thr Ala Ser 
            20                  25                  30          


Ser Ile Glu Phe Val Gly Ser Ser Asn Ser Ser Leu Leu Pro Glu Val 
        35                  40                  45              


Asn Asp His Thr Val Asn Phe Gly Ile Asn Phe Asn Ala Ile Gly Ala 
    50                  55                  60                  


Tyr Gly Asn Val Pro Ser Ser Val Ser Ala Thr Ala Glu Val Thr Ile 
65                  70                  75                  80  


Asn Gly Gln Lys Thr Thr Ile Asn Leu Pro Glu Asn Gln Lys Ser Tyr 
                85                  90                  95      


Ile Tyr Tyr Ala Thr Thr Asn Glu Ser Val Asp Ala Ser Lys Leu Val 
            100                 105                 110         


Ala Gly Gln Lys Tyr Tyr Thr Gly Ile Asn Asn Ala Ser Leu Asn Leu 
        115                 120                 125             


Gly Ser Pro Asn His Asp Lys Asp Ile Thr Leu Glu Gly Ser Asn Val 
    130                 135                 140                 


Ser Phe Lys Thr Asn Asp Ser Asp Pro Tyr Thr Lys Thr Leu Lys Val 
145                 150                 155                 160 


Asn Thr Asp Lys Asn Gly Val Ile Ser Asn Leu Ser Ile Lys Ser Ala 
                165                 170                 175     


Asn Phe Asp Ala Val Asp Val Asn Asn Ala Arg Thr Val Ser Phe Tyr 
            180                 185                 190         


Asp Ala Asp Thr Gly Asn Ile Val Thr Ser Gly Ala Leu Glu Ile Asn 
        195                 200                 205             


Ala Gly Pro Asn Ala Gln Met Asn Val Gln Thr Ile Leu Ala Lys Phe 
    210                 215                 220                 


Glu Gln Lys Tyr Gln Ala Ala Gln Leu Asn Asn Ala Gly Thr Thr Asn 
225                 230                 235                 240 


Asn Val Ser Tyr Asn Asn Asp Leu Ile Ser Thr Thr Pro Ala Asp Leu 
                245                 250                 255     


Ala Ala Gln Leu Lys Lys Ala Gly Tyr Ser Val Asp Asn Asn Gly Tyr 
            260                 265                 270         


Phe Thr Ala Lys His Ser Phe Thr Val Asn Phe Ser Ala Lys Ser Gly 
        275                 280                 285             


Gln Asn Gly Tyr Thr Thr Thr Met Pro Val Thr Val Thr Val Pro Asn 
    290                 295                 300                 


Val Ala Glu Glu Thr Val Pro Ser Gln Ile Arg Thr Val Met His Asn 
305                 310                 315                 320 


Ala Phe Phe Tyr Asp Lys Asn Gly Lys Arg Val Gly Ser Asp Lys Val 
                325                 330                 335     


Thr Arg Tyr Asn Ser Ala Thr Val Ala Met Asn Thr Thr Thr Ile Ile 
            340                 345                 350         


Gly Lys Ala Tyr Tyr Glu Val Ile Glu Asn Gly Lys Ala Thr Gly Lys 
        355                 360                 365             


Phe Ile Asn Ala Ala Asn Ile Asp Gly Thr Lys Arg Thr Leu Lys His 
    370                 375                 380                 


Asn Ala Tyr Val Tyr Lys Ser Ser Lys Lys Arg Ala Asn Lys Val Val 
385                 390                 395                 400 


Leu Lys Lys Gly Glu Thr Val Val Thr Tyr Gly Gly Ala Tyr Thr Phe 
                405                 410                 415     


Lys Asn Gly Lys Gln Tyr Tyr Lys Ile Gly Asn Asn Thr Asp Lys Thr 
            420                 425                 430         


Tyr Val Lys Val Ala Asn Phe 
        435                 


<210>  12
<211>  442
<212>  PRT
<213>  Lactobacillus crispatus

<400>  12

Met Lys Lys Asn Leu Arg Ile Val Ser Ala Ala Ala Ala Ala Leu Leu 
1               5                   10                  15      


Ala Val Ala Pro Val Ala Ala Ser Ala Val Ser Val Asn Ala Ala Ser 
            20                  25                  30          


Ser Ser Ala Val Gln Thr Ala Thr Asn Ile Gly Thr Val Leu Pro Leu 
        35                  40                  45              


Thr Asp Gly Ser Thr Val Asn Val Lys Pro Asn Ile Ser Leu Asn Thr 
    50                  55                  60                  


Ser Ala Tyr Glu Gly Val Lys Ala Asn Ile Ser Val Ser Phe Ser Ala 
65                  70                  75                  80  


Thr Val Asp Gly Thr Thr Ala Thr Ser Asn Phe Thr Pro Asn Ala Ser 
                85                  90                  95      


Thr Ile Glu Leu Trp Lys Asn Glu Lys Asn Lys Val Thr Gln Val Thr 
            100                 105                 110         


Tyr Leu Gln Gln Val Thr Ser Ser Asn Ala Gly Ala Thr Tyr Gln Val 
        115                 120                 125             


Lys Met Thr Gln Val Gly Leu Asn Phe Gly Ser Gln Asn Ala Asn Lys 
    130                 135                 140                 


Lys Val Thr Leu Thr Phe Pro Glu Gly Asp Met Phe Lys Thr Ala Asp 
145                 150                 155                 160 


Thr Ser Leu Ala Gln Ser His Glu Val Lys Leu Asp Gln Asn Gly Thr 
                165                 170                 175     


Ile Thr Leu Pro Glu Val Val Met Asn Val Thr Ala Lys Asp Phe Ala 
            180                 185                 190         


Asn Pro Ala Val Val Asn Trp Tyr Asn Thr Ala Thr Asn Ala Val Val 
        195                 200                 205             


Ser Thr Gly Asn Ile Glu Leu Phe Ala Gly Ser Asp Ala Gly Lys Met 
    210                 215                 220                 


Asn Val Ala Gln Val Val Ser Ala Thr Glu Lys Lys Tyr His Ala Ser 
225                 230                 235                 240 


Asn Tyr Gly Thr Lys Ala Asn Gln Glu Ser Ser Thr Ile Ser Tyr Thr 
                245                 250                 255     


Asn Asn Leu Lys Asp Ala Leu Lys Ala Met Asn Val Asp Val Asp Ala 
            260                 265                 270         


Gln Gly Trp Phe Val Ala Pro Lys Ser Phe Thr Phe Asn Met Thr Ala 
        275                 280                 285             


Lys Ala Asn Asn Asn Asp Ala Ser Ser Thr Leu Ala Val Thr Val Ser 
    290                 295                 300                 


Val Pro Asn Gly Lys Asp Met Thr Val Pro Ser Gln Ser Lys Thr Val 
305                 310                 315                 320 


Met His Asn Ala Phe Phe Tyr Asp Lys Asn Gly Lys Arg Val Gly Ser 
                325                 330                 335     


Asp Lys Val Thr Arg Tyr Asn Ser Ala Thr Val Ala Met Asn Thr Thr 
            340                 345                 350         


Thr Ile Asn Gly Lys Ala Tyr Tyr Glu Val Ile Glu Asn Gly Lys Ala 
        355                 360                 365             


Thr Gly Lys Phe Ile Asn Ala Ala Asn Ile Asp Gly Thr Lys Arg Thr 
    370                 375                 380                 


Leu Lys His Asn Ala Tyr Val Tyr Lys Ser Ser Lys Lys Arg Ala Asn 
385                 390                 395                 400 


Lys Val Val Leu Lys Lys Gly Thr Glu Val Val Thr Tyr Gly Gly Ala 
                405                 410                 415     


Tyr Thr Phe Lys Asn Gly Lys Gln Tyr Tyr Lys Ile Gly Asn Asn Thr 
            420                 425                 430         


Asp Lys Thr Tyr Val Lys Val Ser Asn Phe 
        435                 440         


<210>  13
<211>  322
<212>  PRT
<213>  Lactobacillus helveticus

<400>  13

Ser Val Ser Glu Ser Lys Asp Thr Val Asn Val Thr Pro Ser Phe Thr 
1               5                   10                  15      


Leu Thr Ser Ala Ile Pro Ala Lys Gly Val Pro Ala Thr Leu Gln Gly 
            20                  25                  30          


Ser Ile Glu Ala Ser Leu Asn Gly Thr Ser Val Thr Ala Asp Val Ala 
        35                  40                  45              


Asp Val Ala Lys Asp Val Thr Leu Thr Asp Gly Asn Lys Thr Val Tyr 
    50                  55                  60                  


Ser Tyr Asn Glu Arg Glu Asn Lys Val Asp Asn Asn Leu Ser Ala Val 
65                  70                  75                  80  


Glu Ala Ser Lys Glu Tyr Thr Met Thr Leu Ser Gly Val Gly Phe Ser 
                85                  90                  95      


Phe Gly Lys Ala Asn Ala Gly Lys Thr Leu Thr Phe Lys Leu Pro Lys 
            100                 105                 110         


Asn Val Lys Val Asn Asp Thr Ser Asn Asp Val Lys Val Ser Leu Asp 
        115                 120                 125             


Gln Tyr Gly Asn Ala Thr Asn Leu Lys Phe Val Ile Ser Asn Ile Lys 
    130                 135                 140                 


Ala Tyr Asp Ser Ala Asn Thr Asn Ala Val Ser Phe Tyr Ala Ala Lys 
145                 150                 155                 160 


Ser Gly Leu Val Ala Thr Gln Gly Ser Tyr Met Thr Leu Ala Asp Glu 
                165                 170                 175     


Asn Gly Asn Leu Asn Val Asn Thr Leu Leu Asp Lys Leu Lys Gly Lys 
            180                 185                 190         


Tyr Glu Ala Met Gln Phe Lys Asp Ser Lys Phe Glu Thr Val Asn Val 
        195                 200                 205             


Asn Thr Thr Ala Asp Asp Val Lys Ala Glu Leu Glu Lys Ala Gly Ile 
    210                 215                 220                 


Lys Val Asp Ala Ala Asn Asn Phe Glu Ala Pro Asp Thr Phe Thr Val 
225                 230                 235                 240 


Thr Leu Asn Ala Lys Ser Asp Val Asn Gly Lys Thr Ala Ser Leu Pro 
                245                 250                 255     


Val Val Val Thr Val Pro Asn Gly Lys Ser Thr Val Val Pro Ser Gln 
            260                 265                 270         


Ser Lys Thr Ile Met His Asn Ala Tyr Tyr Tyr Asp Lys Asp Ala Lys 
        275                 280                 285             


Arg Val Gly Thr Asp Lys Val Thr Arg Tyr Asn Ala Val Thr Val Ala 
    290                 295                 300                 


Met Asn Thr Thr Lys Leu Ala Asn Gly Ile Ser Tyr Tyr Glu Val Ile 
305                 310                 315                 320 


Glu Asn 
        


<210>  14
<211>  330
<212>  PRT
<213>  Lactobacillus helveticus

<400>  14

Ala Asp Ser Ala Ile Asn Ala Asn Thr Asn Ala Lys Tyr Asp Val Asp 
1               5                   10                  15      


Val Thr Pro Ser Ile Ser Ala Ile Ala Ala Val Ala Lys Ser Asp Thr 
            20                  25                  30          


Met Pro Ala Ile Pro Gly Ser Leu Thr Gly Ser Ile Ser Ala Ser Tyr 
        35                  40                  45              


Asn Gly Lys Ser Tyr Thr Ala Asn Leu Pro Lys Asp Ser Gly Asn Ala 
    50                  55                  60                  


Thr Ile Thr Asp Ser Asn Asn Asn Thr Val Lys Pro Ala Lys Leu Glu 
65                  70                  75                  80  


Ala Asp Lys Ala Tyr Thr Val Thr Val Pro Asp Val Ser Phe Asn Phe 
                85                  90                  95      


Gly Ser Glu Asn Ala Gly Lys Val Ile Thr Ile Gly Ser Ala Asn Pro 
            100                 105                 110         


Asn Val Thr Phe Thr Lys Lys Thr Gly Asp Gln Pro Ala Ser Thr Val 
        115                 120                 125             


Lys Val Thr Leu Asp Gln Asp Gly Val Ala Lys Leu Ser Ser Val Gln 
    130                 135                 140                 


Ile Lys Asn Val Tyr Ala Ile Asp Thr Thr Tyr Asn Ser Asn Val Asn 
145                 150                 155                 160 


Phe Tyr Asp Val Thr Thr Gly Ala Ile Val Thr Thr Gly Ala Val Ser 
                165                 170                 175     


Ile Asp Ala Asp Asn Gln Gly Gln Leu Asn Ile Thr Ser Val Val Ala 
            180                 185                 190         


Ala Ile Asn Ser Lys Tyr Phe Ala Ala Gln Tyr Asp Lys Lys Gln Leu 
        195                 200                 205             


Thr Asn Asp Val Thr Phe Asp Thr Glu Thr Ala Val Lys Asp Ala Leu 
    210                 215                 220                 


Lys Ala Gln Lys Ile Glu Val Ser Ser Val Gly Tyr Phe Lys Ala Pro 
225                 230                 235                 240 


His Thr Phe Thr Val Asn Val Lys Ala Thr Ser Asn Lys Asn Gly Lys 
                245                 250                 255     


Ser Ala Thr Leu Pro Val Thr Val Thr Val Pro Asn Val Ala Asp Pro 
            260                 265                 270         


Val Val Pro Ser Gln Ser Lys Thr Ile Met His Asn Ala Tyr Phe Tyr 
        275                 280                 285             


Asp Lys Asp Ala Lys Arg Val Gly Thr Asp Lys Val Thr Arg Tyr Asn 
    290                 295                 300                 


Thr Val Thr Val Ala Met Asn Thr Thr Lys Leu Ala Asn Gly Ile Ser 
305                 310                 315                 320 


Tyr Tyr Glu Val Ile Glu Asn Gly Lys Ala 
                325                 330 


<210>  15
<211>  475
<212>  PRT
<213>  Lactobacillus helveticus

<400>  15

Met Asp His Val Ser Lys Gly Phe Val His Tyr Arg Leu Leu Ser His 
1               5                   10                  15      


Ala Glu Pro Met Ala Tyr Tyr Ile Phe Tyr Ile Ser Arg Arg Lys Asp 
            20                  25                  30          


His Met Lys Lys Asn Leu Arg Ile Val Ser Ala Ala Ala Ala Ala Leu 
        35                  40                  45              


Leu Ala Val Ala Pro Val Ala Ala Thr Ala Met Pro Val Asn Ala Ala 
    50                  55                  60                  


Thr Thr Ile Asn Ala Asp Ser Ala Ile Asn Ala Asn Thr Asn Ala Lys 
65                  70                  75                  80  


Tyr Asp Val Asp Val Thr Pro Ser Ile Ser Ala Ile Ala Lys Val Thr 
                85                  90                  95      


Gly Ser Ala Thr Ile Pro Gly Ser Leu Thr Gly Ser Ile Ser Ala Ser 
            100                 105                 110         


Tyr Asn Gly Lys Ser Tyr Thr Ala Asn Leu Pro Lys Asp Ser Gly Asn 
        115                 120                 125             


Ala Thr Ile Ala Asp Lys His Gly Asn Pro Val Lys Pro Ala Asp Leu 
    130                 135                 140                 


Glu Ala Asp Lys Ala Tyr Thr Val Thr Val Pro Asp Val Ser Phe Asn 
145                 150                 155                 160 


Phe Gly Ser Glu Asn Ala Gly Lys Glu Ile Thr Ile Gly Ser Ala Asn 
                165                 170                 175     


Gln Asn Val Thr Phe Thr Thr Lys Asp Ser Gln Ser Gly Ser Thr Val 
            180                 185                 190         


Ser Gly Ser Thr Val Lys Val Thr Leu Asp Gln Asp Gly Val Ala Lys 
        195                 200                 205             


Leu Ser Ser Val Gln Ile Lys Asp Val Tyr Ala Ile Asp Thr Thr Tyr 
    210                 215                 220                 


Asn Ser Asn Val Asn Phe Tyr Asp Val Thr Thr Gly Ala Ile Val Thr 
225                 230                 235                 240 


Thr Gly Ala Val Ser Ile Asp Ala Asp Asn Gln Gly Gln Leu Asn Thr 
                245                 250                 255     


Ala Ser Val Val Ala Ala Ile Ser Ser Lys Tyr Phe Ala Ala Gln Tyr 
            260                 265                 270         


Ala Asp Lys Asn Leu Thr Ser Asp Asn Val Thr Tyr Asn Ile Glu Thr 
        275                 280                 285             


Ala Val Lys Asp Ala Leu Lys Ala Gln Lys Ile Glu Val Ser Ser Val 
    290                 295                 300                 


Gly Tyr Phe Lys Ala Pro His Thr Phe Thr Val Asn Val Lys Ala Thr 
305                 310                 315                 320 


Ser Asn Lys Asn Gly Lys Ser Ala Thr Leu Pro Val Thr Val Thr Val 
                325                 330                 335     


Pro Asn Val Ala Asp Pro Val Val Pro Ser Gln Ser Lys Thr Ile Met 
            340                 345                 350         


His Asn Ala Tyr Phe Tyr Asp Lys Asp Ala Lys Arg Val Gly Thr Asp 
        355                 360                 365             


Lys Val Thr Arg Tyr Asn Thr Val Thr Val Ala Met Asn Thr Thr Lys 
    370                 375                 380                 


Leu Ala Asn Gly Ile Ser Tyr Tyr Glu Val Ile Glu Asn Gly Lys Ala 
385                 390                 395                 400 


Thr Gly Lys Tyr Ile Asn Ala Asp Asn Ile Asp Gly Thr Lys Arg Thr 
                405                 410                 415     


Leu Lys His Asn Ala Tyr Val Tyr Lys Thr Ser Lys Lys Arg Ala Asn 
            420                 425                 430         


Lys Val Val Leu Lys Lys Gly Thr Glu Val Thr Thr Tyr Gly Gly Ser 
        435                 440                 445             


Tyr Lys Phe Lys Asn Gly Lys Lys Tyr Tyr Lys Ile Gly Ala Asp Thr 
    450                 455                 460                 


Lys Lys Thr Tyr Val Arg Val Glu Asn Phe Asp 
465                 470                 475 


<210>  16
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal Peptide of Recombinant SlpA of SEQ ID NO: 5

<400>  16

Met Gln Ser Ser Leu Lys Lys Ser Leu Tyr Leu Gly Leu Ala Ala Leu 
1               5                   10                  15      


Ser Phe Ala Gly Val Ala Ala Val Ser Thr Thr Ala Ser Ala 
            20                  25                  30  


<210>  17
<211>  417
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature Protein of Recombinant SlpA of SEQ ID NO: 5

<400>  17

Lys Ser Arg Met Ala Thr Thr Ile Asn Ala Ser Ser Ser Ala Ile Asn 
1               5                   10                  15      


Thr Asn Thr Asn Ala Lys Tyr Asp Val Asp Val Thr Pro Ser Val Ser 
            20                  25                  30          


Ala Val Ala Ala Asn Thr Ala Asn Asn Thr Pro Ala Ile Ala Gly Asn 
        35                  40                  45              


Leu Thr Gly Thr Ile Ser Ala Ser Tyr Asn Gly Lys Thr Tyr Thr Ala 
    50                  55                  60                  


Asn Leu Lys Ala Asp Thr Glu Asn Ala Thr Ile Thr Ala Ala Gly Ser 
65                  70                  75                  80  


Thr Thr Ala Val Lys Pro Ala Glu Leu Ala Ala Gly Val Ala Tyr Thr 
                85                  90                  95      


Val Thr Val Asn Asp Val Ser Phe Asn Phe Gly Ser Glu Asn Ala Gly 
            100                 105                 110         


Lys Thr Val Thr Leu Gly Ser Ala Asn Ser Asn Val Lys Phe Thr Gly 
        115                 120                 125             


Thr Asn Ser Asp Asn Gln Thr Glu Thr Asn Val Ser Thr Leu Lys Val 
    130                 135                 140                 


Lys Leu Asp Gln Asn Gly Val Ala Ser Leu Thr Asn Val Ser Ile Ala 
145                 150                 155                 160 


Asn Val Tyr Ala Ile Asn Thr Thr Asp Asn Ser Asn Val Asn Phe Tyr 
                165                 170                 175     


Asp Val Thr Ser Gly Ala Thr Val Thr Asn Gly Ala Val Ser Val Asn 
            180                 185                 190         


Ala Asp Asn Gln Gly Gln Val Asn Val Ala Asn Val Val Ala Ala Ile 
        195                 200                 205             


Asn Ser Lys Tyr Phe Ala Ala Gln Tyr Ala Asp Lys Lys Leu Asn Thr 
    210                 215                 220                 


Arg Thr Ala Asn Thr Glu Asp Ala Ile Lys Ala Ala Leu Lys Asp Gln 
225                 230                 235                 240 


Lys Ile Asp Val Asn Ser Val Gly Tyr Phe Lys Ala Pro His Thr Phe 
                245                 250                 255     


Thr Val Asn Val Lys Ala Thr Ser Asn Thr Asn Gly Lys Ser Ala Thr 
            260                 265                 270         


Leu Pro Val Val Val Thr Val Pro Asn Val Ala Glu Pro Thr Val Ala 
        275                 280                 285             


Ser Val Ser Lys Arg Ile Met His Asn Ala Tyr Tyr Tyr Asp Lys Asp 
    290                 295                 300                 


Ala Lys Arg Val Gly Thr Asp Ser Val Lys Arg Tyr Asn Ser Val Ser 
305                 310                 315                 320 


Val Leu Pro Asn Thr Thr Thr Ile Asn Gly Lys Thr Tyr Tyr Gln Val 
                325                 330                 335     


Val Glu Asn Gly Lys Ala Val Asp Lys Tyr Ile Asn Ala Ala Asn Ile 
            340                 345                 350         


Asp Gly Thr Lys Arg Thr Leu Lys His Asn Ala Tyr Val Tyr Ala Ser 
        355                 360                 365             


Ser Lys Lys Arg Ala Asn Lys Val Val Leu Lys Lys Gly Glu Val Val 
    370                 375                 380                 


Thr Thr Tyr Gly Ala Ser Tyr Thr Phe Lys Asn Gly Gln Lys Tyr Tyr 
385                 390                 395                 400 


Lys Ile Gly Asp Asn Thr Asp Lys Thr Tyr Val Lys Val Ala Asn Phe 
                405                 410                 415     


Arg 
    


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer

<400>  18
aatgcaacca taactgccgc                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  19
tgttgcgcct gaggtaacat                                                   20


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer

<400>  20
ccagctattg ccggtaacct t                                                 21


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  21
aacggcagta gtgctaccag                                                   20


