               SEQUENCE LISTING

<110> Academia Sinica

<120> A PHAGE-DISPLAYED SINGLE-CHAIN VARIABLE FRAGMENT LIBRARY FOR SELECTING ANTIBODY FRAGMENTS SPECIFIC TO MESOTHELIN

<130> P4191-PCT

<150> US63208496
<151> 2021-06-08

<160> 46

<170> BiSSAP 1.3

<210> 1
<211> 63
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthesized_CDR-L1 primer

<220> 
<221> variation
<222> 31,32,34,35,37,38
<223> /note="n is A, T, C, or G"

<400> 1
accattacct gccgtgcgag ccaggatgtt nnknnknnkg tcgcatggta tcagcagaaa     60

cca                                                                   63


<210> 2
<211> 69
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthesized_CDR-L2 primer

<220> 
<221> variation
<222> 25,26,28,29,31,32,34,35,37,38
<223> /note="n is A, T, C, or G"

<400> 2
ggcaaagcgc cgaaacttct gatannknnk nnknnknnkc tgtatagcgg cgtgccgtcg     60

cgtttttcg                                                             69


<210> 3
<211> 69
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthesized_CDR-L3 primer

<220> 
<221> variation
<222> 28,29,31,32,34,35,37,38,43,44
<223> /note="n is A, T, C, or G"

<400> 3
gattttgcga cctactactg tcaacagnnk nnknnknnkc cgnnkacctt cggtcaaggc     60

accaaagtg                                                             69


<210> 4
<211> 63
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthesized_CDR-H1 primer

<220> 
<221> variation
<222> 28,29,31,32,34,35,37,38
<223> /note="n is A, T, C, or G"

<400> 4
agctgtgcgg cgagcgggtt caccattnnk nnknnknnka ttcattgggt gcgtcaagct     60

ccc                                                                   63


<210> 5
<211> 81
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthesized_CDR-H2 primer

<220> 
<221> variation
<222> 22,23,28,29,34,35,37,38,43,44,49,50
<223> /note="n is A, T, C, or G"

<400> 5
aaggggctgg agtgggtcgc gnnkattnnk cccnnknnkg gtnnkacann ktatgccgac     60

agcgtgaaag gtcgctttac g                                               81


<210> 6
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthesized_CDR-H3 primer

<220> 
<221> variation
<222> 25,26,28,29,31,32,34,35,37,38
<223> /note="n is A, T, C, or G"

<400> 6
acagcggtgt attattgcgc gcgtnnknnk nnknnknnkg attattgggg gcagggcacc     60

cttgtt                                                                66


<210> 7
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS5_CDR-L1

<400> 7
Arg Ala Ser Gln Asp Val Lys Glu Gly Val Ala 
1               5                   10      

<210> 8
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS5_CDR-L2

<400> 8
Asp Gln Ser His Arg Leu Tyr Ser 
1               5               

<210> 9
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS5_CDR-L3

<400> 9
Gln Gln Tyr Tyr Ala Trp Pro Ser Thr 
1               5                   

<210> 10
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS5_CDR-H1

<400> 10
Ala Ala Ser Gly Phe Thr Ile Thr Asp Arg Thr Ile His 
1               5                   10              

<210> 11
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS5_CDR-H2

<400> 11
Ser Ile Phe Pro Thr Lys Gly Val Thr Thr 
1               5                   10  

<210> 12
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS5_CDR-H3

<400> 12
Ala Arg Gly Arg Tyr Trp Met Asp Tyr 
1               5                   

<210> 13
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS7_CDR-L1

<400> 13
Arg Ala Ser Gln Asp Val Glu Asp Gly Val Ala 
1               5                   10      

<210> 14
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS7_CDR-L2

<400> 14
Gly Ile Lys Asp Leu Leu Tyr Ser 
1               5               

<210> 15
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS7_CDR-L3

<400> 15
Gln Gln Tyr Tyr Arg Trp Pro Ser Thr 
1               5                   

<210> 16
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS7_CDR-H1

<400> 16
Ala Ala Ser Gly Phe Thr Ile Asp Lys Glu Ala Ile His 
1               5                   10              

<210> 17
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS7_CDR-H2

<400> 17
Ser Ile Tyr Pro His Ser Gly Phe Thr Leu 
1               5                   10  

<210> 18
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS8_CDR-L2

<400> 18
Asp Glu Arg Glu Arg Leu Tyr Ser 
1               5               

<210> 19
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS8_CDR-L3

<400> 19
Gln Gln Tyr Asn Thr Trp Pro Ala Thr 
1               5                   

<210> 20
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS8_CDR-H1

<400> 20
Ala Ala Ser Gly Phe Thr Ile Ser Lys Ala Ser Ile His 
1               5                   10              

<210> 21
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS8_CDR-H2

<400> 21
Ser Ile Trp Pro Thr Lys Gly Phe Thr Thr 
1               5                   10  

<210> 22
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS8_CDR-H3

<400> 22
Ala Arg Gly Arg Tyr Trp Leu Asp Tyr 
1               5                   

<210> 23
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_ALA12_CDR-L1

<400> 23
Arg Ala Ser Gln Asp Val Arg Asp Gly Val Ala 
1               5                   10      

<210> 24
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_ALA12_CDR-L2

<400> 24
Asp Gln Met Val Arg Leu Tyr Ser 
1               5               

<210> 25
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_ALA12_CDR-L3

<400> 25
Gln Gln Tyr Phe Asn Trp Pro Val Thr 
1               5                   

<210> 26
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_ALA12_CDR-H1

<400> 26
Ala Ala Ser Gly Phe Thr Ile Gly Asn Trp Thr Ile His 
1               5                   10              

<210> 27
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_ALA12_CDR-H2

<400> 27
Leu Ile Trp Pro Glu Ser Gly Ala Thr Leu 
1               5                   10  

<210> 28
<211> 108
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS5_VL domain

<400> 28
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Lys Glu Gly 
            20                  25                  30          
Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Asp Gln Ser His Arg Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ala Trp Pro Ser 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105             

<210> 29
<211> 116
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS5_VH domain

<400> 29
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Ile Thr Asp Arg 
            20                  25                  30          
Thr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Ala Ser Ile Phe Pro Thr Lys Gly Val Thr Thr Tyr Ala Asp Ser Val 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Gly Arg Tyr Trp Met Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         
Thr Val Ser Ser 
        115     

<210> 30
<211> 108
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS7_VL domain

<400> 30
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Glu Asp Gly 
            20                  25                  30          
Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Gly Ile Lys Asp Leu Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Arg Trp Pro Ser 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105             

<210> 31
<211> 116
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS7_VH domain

<400> 31
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Ile Asp Lys Glu 
            20                  25                  30          
Ala Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Ala Ser Ile Tyr Pro His Ser Gly Phe Thr Leu Tyr Ala Asp Ser Val 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Gly Arg Tyr Trp Met Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         
Thr Val Ser Ser 
        115     

<210> 32
<211> 108
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS8_VL domain

<400> 32
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Lys Glu Gly 
            20                  25                  30          
Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Asp Glu Arg Glu Arg Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Thr Trp Pro Ala 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105             

<210> 33
<211> 116
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_CHS8_VH domain

<400> 33
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Ile Ser Lys Ala 
            20                  25                  30          
Ser Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Ala Ser Ile Trp Pro Thr Lys Gly Phe Thr Thr Tyr Ala Asp Ser Val 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Gly Arg Tyr Trp Leu Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         
Thr Val Ser Ser 
        115     

<210> 34
<211> 108
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_ALA12_VL domain

<400> 34
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Arg Asp Gly 
            20                  25                  30          
Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Asp Gln Met Val Arg Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Phe Asn Trp Pro Val 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105             

<210> 35
<211> 116
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_ALA12_VH domain

<400> 35
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Ile Gly Asn Trp 
            20                  25                  30          
Thr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Ala Leu Ile Trp Pro Glu Ser Gly Ala Thr Leu Tyr Ala Asp Ser Val 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Gly Arg Tyr Trp Met Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         
Thr Val Ser Ser 
        115     

<210> 36
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_ER retention peptide

<400> 36
Lys Asp Glu Leu 
1               

<210> 37
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_first polypeptide

<400> 37
Ala Ser Ala Ala Gly Gly Ser Gly Thr 
1               5                   

<210> 38
<211> 138
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_adaptor

<400> 38
Tyr Asn Lys Asp Gln Gln Ser Ala Phe Tyr Glu Ile Leu Asn Met Pro 
1               5                   10                  15      
Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp 
            20                  25                  30          
Asp Pro Ser Gln Ser Thr Asn Val Leu Gly Glu Ala Lys Lys Leu Asn 
        35                  40                  45              
Glu Ser Gln Ala Pro Lys Ala Asp Asn Gly Gly Gly Ser Gly Gly Gly 
    50                  55                  60                  
Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Glu Val Thr Ile 
65                  70                  75                  80  
Lys Val Asn Leu Ile Phe Ala Asp Gly Lys Ile Gln Thr Ala Glu Phe 
                85                  90                  95      
Lys Gly Thr Phe Glu Glu Ala Thr Ala Glu Ala Tyr Arg Tyr Ala Ala 
            100                 105                 110         
Leu Leu Ala Lys Val Asn Gly Glu Tyr Thr Ala Asp Leu Glu Asp Gly 
        115                 120                 125             
Gly Asn His Met Asn Ile Lys Phe Ala Gly 
    130                 135             

<210> 39
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_M9_CDR-L1

<400> 39
Arg Ala Ser Gln Asp Val Asn Asp Gly Val Ala 
1               5                   10      

<210> 40
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_M9_CDR-L2

<400> 40
Ser Gly Ser Pro Trp Leu Tyr Ser 
1               5               

<210> 41
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_M9_CDR-L3

<400> 41
Gln Gln Tyr Phe Asn Trp Pro Ile Thr 
1               5                   

<210> 42
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_M9_CDR-H1

<400> 42
Ala Ala Ser Gly Phe Thr Ile Asp Asn Tyr Gly Ile His 
1               5                   10              

<210> 43
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_M9_CDR-H2

<400> 43
Trp Ile Trp Pro Tyr Gly Gly Ser Thr Tyr 
1               5                   10  

<210> 44
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_M9_CDR-H3

<400> 44
Ala Arg Gly Tyr Tyr Trp Tyr Asp Tyr 
1               5                   

<210> 45
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_type I amino acid sequence

<400> 45
Ser Thr Asn Gln Gly 
1               5   

<210> 46
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthesized_type II amino acid sequence

<400> 46
Lys Arg His Asp Glu 
1               5   

