SEQUENCE LISTING

<110> UNIVERSITY OF MARYLAND, BALTIMORE

<120> C2 Domain Therapeutics and Uses Thereof

<130> D7899PCT

<141> 2022-06-07

<150> US 63/197,550
<151> 2021-06-07

<160> 17

<210> 1
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence for pV-C2Astop and pV-2xC2Astop plasmids

<400> 1
cgacggtacc actagtacgc gtatg     25

<210> 2
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence for pV-C2Astop plasmid

<400> 2
atcagatatc tcagctgaag ggcttcacca gcacagctcc aggcagcggt 50
gtgtag                                                 56

<210> 3
<211> 1191
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence for pV-C2Astop plasmid

<400> 3
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt   50 
cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg  100
gcgagggcga tgccacctac ggcaagctga ccctgaagct catctgcacc  150
accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctcggcta  200
cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact  250
tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc  300
ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg  350
cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg  400
acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac  450
gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa  500
gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc  550
agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac  600
tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga  650
tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca  700
tggacgagct gtacaagtcc ggactcagat ctcgagctca agcttcgaat  750
tctgcagtcg acggtaccac tagtacgcgt atgctgaggg tcttcatcct  800
ctatgccgag aacgtccaca cacccgacac cgacatcagc gatgcctact  850
gctccgcggt gtttgcaggg gtgaagaaga gaaccaaagt catcaagaac  900
agcgtgaacc ctgtatggaa tgagggattt gaatgggacc tcaagggcat  950
ccccctggac cagggctctg agcttcatgt ggtggtcaaa gaccatgaga 1000
cgatggggag gaacaggttc ctgggggaag ccaaggtccc actccgagag 1050
gtcctcgcca cccctagtct gtccgccagc ttcaatgccc ccctgctgga 1100
caccaagaag cagcccacag gggcctcgct ggtcctgcag gtgtcctaca 1150
caccgctgcc tggagctgtg ctggtgaagc ccttcagctg a          1191

<210> 4
<211> 396
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for pV-C2Astop plasmid

<400> 4
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 
1               5                   10                  15
Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val 
                20                  25                  30
Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 
                35                  40                  45
Lys Leu Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr 
                50                  55                  60
Leu Val Thr Thr Leu Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr 
                65                  70                  75
Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro 
                80                  85                  90
Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly 
                95                  100                 105
Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
                110                 115                 120
Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
                125                 130                 135
Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn 
                140                 145                 150
Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn 
                155                 160                 165
Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly Val Gln Leu Ala 
                170                 175                 180
Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
                185                 190                 195
Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser Lys 
                200                 205                 210
Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
                215                 220                 225
Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
                230                 235                 240
Gly Leu Arg Ser Arg Ala Gln Ala Ser Asn Ser Ala Val Asp Gly 
                245                 250                 255
Thr Thr Ser Thr Arg Met Leu Arg Val Phe Ile Leu Tyr Ala Glu 
                260                 265                 270
Asn Val His Thr Pro Asp Thr Asp Ile Ser Asp Ala Tyr Cys Ser 
                275                 280                 285
Ala Val Phe Ala Gly Val Lys Lys Arg Thr Lys Val Ile Lys Asn 
                290                 295                 300
Ser Val Asn Pro Val Trp Asn Glu Gly Phe Glu Trp Asp Leu Lys 
                305                 310                 315
Gly Ile Pro Leu Asp Gln Gly Ser Glu Leu His Val Val Val Lys 
                320                 325                 330
Asp His Glu Thr Met Gly Arg Asn Arg Phe Leu Gly Glu Ala Lys 
                335                 340                 345
Val Pro Leu Arg Glu Val Leu Ala Thr Pro Ser Leu Ser Ala Ser 
                350                 355                 360
Phe Asn Ala Pro Leu Leu Asp Thr Lys Lys Gln Pro Thr Gly Ala 
                365                 370                 375
Ser Leu Val Leu Gln Val Ser Tyr Thr Pro Leu Pro Gly Ala Val 
                380                 385                 390
Leu Val Lys Pro Phe Ser
                395

<210> 5
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence for pV-2xC2Astop plasmid

<400> 5
gctaggtacc gctgaagggc ttcaccagca c    31

<210> 6
<211> 1617
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence for pV-2xC2Astop plasmid

<400> 6
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt   50
cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg  100
gcgagggcga tgccacctac ggcaagctga ccctgaagct catctgcacc  150
accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctcggcta  200
cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact  250
tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc  300
ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg  350
cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg  400
acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac  450
gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa  500
gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc  550
agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac  600
tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga  650
tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca  700
tggacgagct gtacaagtcc ggactcagat ctcgagctca agcttcgaat  750
tctgcagtcg acggtaccac tagtacgcgt atgctgaggg tcttcatcct  800
ctatgccgag aacgtccaca cacccgacac cgacatcagc gatgcctact  850
gctccgcggt gtttgcaggg gtgaagaaga gaaccaaagt catcaagaac  900
agcgtgaacc ctgtatggaa tgagggattt gaatgggacc tcaagggcat  950
ccccctggac cagggctctg agcttcatgt ggtggtcaaa gaccatgaga 1000
cgatggggag gaacaggttc ctgggggaag ccaaggtccc actccgagag 1050
gtcctcgcca cccctagtct gtccgccagc ttcaatgccc ccctgctgga 1100
caccaagaag cagcccacag gggcctcgct ggtcctgcag gtgtcctaca 1150
caccgctgcc tggagctgtg ctggtgaagc ccttcagcgg taccactagt 1200
acgcgtatgc tgagggtctt catcctctat gccgagaacg tccacacacc 1250
cgacaccgac atcagcgatg cctactgctc cgcggtgttt gcaggggtga 1300
agaagagaac caaagtcatc aagaacagcg tgaaccctgt atggaatgag 1350
ggatttgaat gggacctcaa gggcatcccc ctggaccagg gctctgagct 1400
tcatgtggtg gtcaaagacc atgagacgat ggggaggaac aggttcctgg 1450
gggaagccaa ggtcccactc cgagaggtcc tcgccacccc tagtctgtcc 1500
gccagcttca atgcccccct gctggacacc aagaagcagc ccacaggggc 1550
ctcgctggtc ctgcaggtgt cctacacacc gctgcctgga gctgtgctgg 1600
tgaagccctt cagctga                                     1617

<210> 7
<211> 538
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for pV-2xC2Astop plasmid

<400> 7
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 
1               5                   10                  15
Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val 
                20                  25                  30
Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 
                35                  40                  45
Lys Leu Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr 
                50                  55                  60
Leu Val Thr Thr Leu Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr 
                65                  70                  75
Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro 
                80                  85                  90
Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly 
                95                  100                 105
Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
                110                 115                 120
Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
                125                 130                 135
Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn 
                140                 145                 150
Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn 
                155                 160                 165
Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly Val Gln Leu Ala 
                170                 175                 180
Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
                185                 190                 195
Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser Lys 
                200                 205                 210
Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
                215                 220                 225
Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
                230                 235                 240
Gly Leu Arg Ser Arg Ala Gln Ala Ser Asn Ser Ala Val Asp Gly 
                245                 250                 255
Thr Thr Ser Thr Arg Met Leu Arg Val Phe Ile Leu Tyr Ala Glu 
                260                 265                 270
Asn Val His Thr Pro Asp Thr Asp Ile Ser Asp Ala Tyr Cys Ser 
                275                 280                 285
Ala Val Phe Ala Gly Val Lys Lys Arg Thr Lys Val Ile Lys Asn 
                290                 295                 300
Ser Val Asn Pro Val Trp Asn Glu Gly Phe Glu Trp Asp Leu Lys 
                305                 310                 315
Gly Ile Pro Leu Asp Gln Gly Ser Glu Leu His Val Val Val Lys 
                320                 325                 330
Asp His Glu Thr Met Gly Arg Asn Arg Phe Leu Gly Glu Ala Lys 
                335                 340                 345
Val Pro Leu Arg Glu Val Leu Ala Thr Pro Ser Leu Ser Ala Ser 
                350                 355                 360
Phe Asn Ala Pro Leu Leu Asp Thr Lys Lys Gln Pro Thr Gly Ala 
                365                 370                 375
Ser Leu Val Leu Gln Val Ser Tyr Thr Pro Leu Pro Gly Ala Val 
                380                 385                 390
Leu Val Lys Pro Phe Ser Gly Thr Thr Ser Thr Arg Met Leu Arg 
                395                 400                 405
Val Phe Ile Leu Tyr Ala Glu Asn Val His Thr Pro Asp Thr Asp 
                410                 415                 420
Ile Ser Asp Ala Tyr Cys Ser Ala Val Phe Ala Gly Val Lys Lys 
                425                 430                 435
Arg Thr Lys Val Ile Lys Asn Ser Val Asn Pro Val Trp Asn Glu 
                440                 445                 450
Gly Phe Glu Trp Asp Leu Lys Gly Ile Pro Leu Asp Gln Gly Ser 
                455                 460                 465
Glu Leu His Val Val Val Lys Asp His Glu Thr Met Gly Arg Asn 
                470                 475                 480
Arg Phe Leu Gly Glu Ala Lys Val Pro Leu Arg Glu Val Leu Ala 
                485                 490                 495
Thr Pro Ser Leu Ser Ala Ser Phe Asn Ala Pro Leu Leu Asp Thr 
                500                 505                 510
Lys Lys Gln Pro Thr Gly Ala Ser Leu Val Leu Gln Val Ser Tyr 
                515                 520                 525
Thr Pro Leu Pro Gly Ala Val Leu Val Lys Pro Phe Ser
                530                 535

<210> 8
<211> 46
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence for pV-C2pkc-C2Astop and 
      pV-2xC2pkc-C2Astop plasmids

<400> 8
cgacggtacc actagtacgc gtatggagaa gagggggcgg atttac    46

<210> 9
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence for pV-C2pkc-C2Astop and 
      pV-2xC2pkc-C2Astop plasmids

<400> 9
cgacggtacc gttgccagca gggccaagtt tg    32

<210> 10
<211> 1689
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence for pV-C2pkc-C2Astop plasmid

<400> 10
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt   50
cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg  100
gcgagggcga tgccacctac ggcaagctga ccctgaagct catctgcacc  150
accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctcggcta  200
cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact  250
tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc  300
ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg  350
cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg  400
acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac  450
gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa  500
gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc  550
agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac  600
tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga  650
tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca  700
tggacgagct gtacaagtcc ggactcagat ctcgagctca agcttcgaat  750
tctgcagtcg acggtaccac tagtacgcgt atggagaaga gggggcggat  800
ttacctaaag gctgaggttg ctgatgaaaa gctccatgtc acagtacgag  850
atgcaaaaaa tctaatccct atggatccaa acgggctttc agatccttat  900
gtgaagctga aacttattcc tgatcccaag aatgaaagca agcaaaaaac  950
caaaaccatc cgctccacac taaatccgca gtggaatgag tcctttacat 1000
tcaaattgaa accttcagac aaagaccgac gactgtctgt agaaatctgg 1050
gactgggatc gaacaacaag gaatgacttc atgggatccc tttcctttgg 1100
agtttcggag ctgatgaaga tgccggccag tggatggtac aagttgctta 1150
accaagaaga aggtgagtac tacaacgtac ccattccgga aggggacgag 1200
gaaggaaaca tggaactcag gcagaaattc gagaaagcca aacttggccc 1250
tgctggcaac ggtaccacta gtacgcgtat gctgagggtc ttcatcctct 1300
atgccgagaa cgtccacaca cccgacaccg acatcagcga tgcctactgc 1350
tccgcggtgt ttgcaggggt gaagaagaga accaaagtca tcaagaacag 1400
cgtgaaccct gtatggaatg agggatttga atgggacctc aagggcatcc 1450
ccctggacca gggctctgag cttcatgtgg tggtcaaaga ccatgagacg 1500
atggggagga acaggttcct gggggaagcc aaggtcccac tccgagaggt 1550
cctcgccacc cctagtctgt ccgccagctt caatgccccc ctgctggaca 1600
ccaagaagca gcccacaggg gcctcgctgg tcctgcaggt gtcctacaca 1650
ccgctgcctg gagctgtgct ggtgaagccc ttcagctga             1689

<210> 11
<211> 562
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for pV-C2pkc-C2Astop plasmid

<400> 11
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 
1               5                   10                  15
Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val 
                20                  25                  30
Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 
                35                  40                  45
Lys Leu Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr 
                50                  55                  60
Leu Val Thr Thr Leu Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr 
                65                  70                  75
Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro 
                80                  85                  90
Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly 
                95                  100                 105
Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
                110                 115                 120
Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
                125                 130                 135
Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn 
                140                 145                 150
Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn 
                155                 160                 165
Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly Val Gln Leu Ala 
                170                 175                 180
Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
                185                 190                 195
Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser Lys 
                200                 205                 210
Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
                215                 220                 225
Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
                230                 235                 240
Gly Leu Arg Ser Arg Ala Gln Ala Ser Asn Ser Ala Val Asp Gly 
                245                 250                 255
Thr Thr Ser Thr Arg Met Glu Lys Arg Gly Arg Ile Tyr Leu Lys 
                260                 265                 270
Ala Glu Val Ala Asp Glu Lys Leu His Val Thr Val Arg Asp Ala 
                275                 280                 285
Lys Asn Leu Ile Pro Met Asp Pro Asn Gly Leu Ser Asp Pro Tyr 
                290                 295                 300
Val Lys Leu Lys Leu Ile Pro Asp Pro Lys Asn Glu Ser Lys Gln 
                305                 310                 315
Lys Thr Lys Thr Ile Arg Ser Thr Leu Asn Pro Gln Trp Asn Glu 
                320                 325                 330
Ser Phe Thr Phe Lys Leu Lys Pro Ser Asp Lys Asp Arg Arg Leu 
                335                 340                 345
Ser Val Glu Ile Trp Asp Trp Asp Arg Thr Thr Arg Asn Asp Phe 
                350                 355                 360
Met Gly Ser Leu Ser Phe Gly Val Ser Glu Leu Met Lys Met Pro 
                365                 370                 375
Ala Ser Gly Trp Tyr Lys Leu Leu Asn Gln Glu Glu Gly Glu Tyr 
                380                 385                 390
Tyr Asn Val Pro Ile Pro Glu Gly Asp Glu Glu Gly Asn Met Glu 
                395                 400                 405
Leu Arg Gln Lys Phe Glu Lys Ala Lys Leu Gly Pro Ala Gly Asn  
                410                 415                 420
Gly Thr Thr Ser Thr Arg Met Leu Arg Val Phe Ile Leu Tyr Ala 
                425                 430                 435
Glu Asn Val His Thr Pro Asp Thr Asp Ile Ser Asp Ala Tyr Cys 
                440                 445                 450
Ser Ala Val Phe Ala Gly Val Lys Lys Arg Thr Lys Val Ile Lys 
                455                 460                 465
Asn Ser Val Asn Pro Val Trp Asn Glu Gly Phe Glu Trp Asp Leu 
                470                 475                 480
Lys Gly Ile Pro Leu Asp Gln Gly Ser Glu Leu His Val Val Val 
                485                 490                 495
Lys Asp His Glu Thr Met Gly Arg Asn Arg Phe Leu Gly Glu Ala 
                500                 505                 510
Lys Val Pro Leu Arg Glu Val Leu Ala Thr Pro Ser Leu Ser Ala 
                515                 520                 525
Ser Phe Asn Ala Pro Leu Leu Asp Thr Lys Lys Gln Pro Thr Gly 
                530                 535                 540
Ala Ser Leu Val Leu Gln Val Ser Tyr Thr Pro Leu Pro Gly Ala 
                545                 550                 555
Val Leu Val Lys Pro Phe Ser
                560

<210> 12
<211> 2186
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence for pV-2xC2pkc-C2Astop plasmid

<400> 12
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt   50
cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg  100
gcgagggcga tgccacctac ggcaagctga ccctgaagct catctgcacc  150
accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctcggcta  200
cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact  250
tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc  300
ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg  350
cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg  400
acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac  450
gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa  500
gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc  550
agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac  600
tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga  650
tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca  700
tggacgagct gtacaagtcc ggactcagat ctcgagctca agcttcgaat  750
tctgcagtcg acggtaccac tagtacgcgt atggagaaga gggggcggat  800
ttacctaaag gctgaggttg ctgatgaaaa gctccatgtc acagtacgag  850
atgcaaaaaa tctaatccct atggatccaa acgggctttc agatccttat  900
gtgaagctga aacttattcc tgatcccaag aatgaaagca agcaaaaaac  950
caaaaccatc cgctccacac taaatccgca gtggaatgag tcctttacat 1000
tcaaattgaa accttcagac aaagaccgac gactgtctgt agaaatctgg 1050
gactgggatc gaacaacaag gaatgacttc atgggatccc tttcctttgg 1100
agtttcggag ctgatgaaga tgccggccag tggatggtac aagttgctta 1150
accaagaaga aggtgagtac tacaacgtac ccattccgga aggggacgag 1200
gaaggaaaca tggaactcag gcagaaattc gagaaagcca aacttggccc 1250
tgctggcaac ggtaccacta gtacgcgtat ggagaagagg gggcggattt 1300
acctaaaggc tgaggttgct gatgaaaagc tccatgtcac agtacgagat 1350
gcaaaaaatc taatccctat ggatccaaac gggctttcag atccttatgt 1400
gaagctgaaa cttattcctg atcccaagaa tgaaagcaag caaaaaacca 1450
aaaccatccg ctccacacta aatccgcagt ggaatgagtc ctttacattc 1500
aaattgaaac cttcagacaa agaccgacga ctgtctgtag aaatctggga 1550
tgggatcgaa caacaaggaa tgacttcatg ggatcccttt cctttggagt 1600
ttcggagctg atgaagatgc cggccagtgg atggtacaag ttgcttaacc 1650
aagaagaagg tgagtactac aacgtaccca ttccggaagg ggacgaggaa 1700
ggaaacatgg aactcaggca gaaattcgag aaagccaaac ttggccctgc 1750
tggcaacggt accactagta cgcgtatgct gagggtcttc atcctctatg 1800
ccgagaacgt ccacacaccc gacaccgaca tcagcgatgc ctactgctcc 1850
gcggtgtttg caggggtgaa gaagagaacc aaagtcatca agaacagcgt 1900
gaaccctgta tggaatgagg gatttgaatg ggacctcaag ggcatccccc 1950
tggaccaggg ctctgagctt catgtggtgg tcaaagacca tgagacgatg 2000
gggaggaaca ggttcctggg ggaagccaag gtcccactcc gagaggtcct 2050
cgccacccct agtctgtccg ccagcttcaa tgcccccctg ctggacacca 2100
agaagcagcc cacaggggcc tcgctggtcc tgcaggtgtc ctacacaccg 2150
ctgcctggag ctgtgctggt gaagcccttc agctga                2186

<210> 13
<211> 728
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for pV-2xC2pkc-C2Astop plasmid

<400> 13
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 
1               5                   10                  15
Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val 
                20                  25                  30
Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 
                35                  40                  45
Lys Leu Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr 
                50                  55                  60
Leu Val Thr Thr Leu Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr 
                65                  70                  75
Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro 
                80                  85                  90
Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly 
                95                  100                 105
Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
                110                 115                 120
Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
                125                 130                 135
Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn 
                140                 145                 150
Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn 
                155                 160                 165
Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly Val Gln Leu Ala 
                170                 175                 180
Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
                185                 190                 195
Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser Lys 
                200                 205                 210
Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
                215                 220                 225
Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
                230                 235                 240
Gly Leu Arg Ser Arg Ala Gln Ala Ser Asn Ser Ala Val Asp Gly 
                245                 250                 255
Thr Thr Ser Thr Arg Met Glu Lys Arg Gly Arg Ile Tyr Leu Lys 
                260                 265                 270
Ala Glu Val Ala Asp Glu Lys Leu His Val Thr Val Arg Asp Ala 
                275                 280                 285
Lys Asn Leu Ile Pro Met Asp Pro Asn Gly Leu Ser Asp Pro Tyr 
                290                 295                 300
Val Lys Leu Lys Leu Ile Pro Asp Pro Lys Asn Glu Ser Lys Gln 
                305                 310                 315
Lys Thr Lys Thr Ile Arg Ser Thr Leu Asn Pro Gln Trp Asn Glu 
                320                 325                 330
Ser Phe Thr Phe Lys Leu Lys Pro Ser Asp Lys Asp Arg Arg Leu 
                335                 340                 345
Ser Val Glu Ile Trp Asp Trp Asp Arg Thr Thr Arg Asn Asp Phe 
                350                 355                 360
Met Gly Ser Leu Ser Phe Gly Val Ser Glu Leu Met Lys Met Pro 
                365                 370                 375
Ala Ser Gly Trp Tyr Lys Leu Leu Asn Gln Glu Glu Gly Glu Tyr 
                380                 385                 390
Tyr Asn Val Pro Ile Pro Glu Gly Asp Glu Glu Gly Asn Met Glu 
                395                 400                 405
Leu Arg Gln Lys Phe Glu Lys Ala Lys Leu Gly Pro Ala Gly Asn 
                410                 415                 420
Gly Thr Thr Ser Thr Arg Met Glu Lys Arg Gly Arg Ile Tyr Leu 
                425                 430                 435
Lys Ala Glu Val Ala Asp Glu Lys Leu His Val Thr Val Arg Asp 
                440                 445                 450
Ala Lys Asn Leu Ile Pro Met Asp Pro Asn Gly Leu Ser Asp Pro 
                455                 460                 465
Tyr Val Lys Leu Lys Leu Ile Pro Asp Pro Lys Asn Glu Ser Lys 
                470                 475                 480
Gln Lys Thr Lys Thr Ile Arg Ser Thr Leu Asn Pro Gln Trp Asn 
                485                 490                 495
Glu Ser Phe Thr Phe Lys Leu Lys Pro Ser Asp Lys Asp Arg Arg 
                500                 505                 510
Leu Ser Val Glu Ile Trp Asp Trp Asp Arg Thr Thr Arg Asn Asp 
                515                 520                 525
Phe Met Gly Ser Leu Ser Phe Gly Val Ser Glu Leu Met Lys Met 
                530                 535                 540
Pro Ala Ser Gly Trp Tyr Lys Leu Leu Asn Gln Glu Glu Gly Glu 
                545                 550                 555
Tyr Tyr Asn Val Pro Ile Pro Glu Gly Asp Glu Glu Gly Asn Met 
                560                 565                 570
Glu Leu Arg Gln Lys Phe Glu Lys Ala Lys Leu Gly Pro Ala Gly 
                575                 580                 585
Asn Gly Thr Thr Ser Thr Arg Met Leu Arg Val Phe Ile Leu Tyr 
                590                 595                 600
Ala Glu Asn Val His Thr Pro Asp Thr Asp Ile Ser Asp Ala Tyr 
                605                 610                 615
Cys Ser Ala Val Phe Ala Gly Val Lys Lys Arg Thr Lys Val Ile 
                620                 625                 630
Lys Asn Ser Val Asn Pro Val Trp Asn Glu Gly Phe Glu Trp Asp 
                635                 640                 645
Leu Lys Gly Ile Pro Leu Asp Gln Gly Ser Glu Leu His Val Val 
                650                 655                 660
Val Lys Asp His Glu Thr Met Gly Arg Asn Arg Phe Leu Gly Glu 
                665                 670                 675
Ala Lys Val Pro Leu Arg Glu Val Leu Ala Thr Pro Ser Leu Ser 
                680                 685                 690
Ala Ser Phe Asn Ala Pro Leu Leu Asp Thr Lys Lys Gln Pro Thr 
                695                 700                 705
Gly Ala Ser Leu Val Leu Gln Val Ser Tyr Thr Pro Leu Pro Gly 
                710                 715                 720
Ala Val Leu Val Lys Pro Phe Ser
                725

<210> 14
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence for pV-2xC2pkc-2xC2Astop plasmid

<400> 14
cgagctgtac aagtccggac tcgtcgacag atctac    36

<210> 15
<211> 38
<212> dna
<213> artificial sequence

<220> 
<223> primer sequence for pv-2xc2pkc-2xc2astop plasmid

<400> 15
cgactgcaga attcgaagct ttcagtcgac gttgccag    38

<210> 16
<211> 2619
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence for pV-2xC2pkc-2xC2Astop plasmid

<400> 16
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt   50
cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg  100
gcgagggcga tgccacctac ggcaagctga ccctgaagct catctgcacc  150
accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctcggcta  200
cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact  250
tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc  300
ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg  350
cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg  400
acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac  450
gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa  500
gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc  550
agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac  600
tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga  650
tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca  700
tggacgagct gtacaagtcc ggactcagat ctcgagctca agcttcgaat  750
tctgcagtcg acagatctac tagtacgcgt atggagaaga gggggcggat  800
ttacctaaag gctgaggttg ctgatgaaaa gctccatgtc acagtacgag  850
atgcaaaaaa tctaatccct atggatccaa acgggctttc agatccttat  900
gtgaagctga aacttattcc tgatcccaag aatgaaagca agcaaaaaac  950
caaaaccatc cgctccacac taaatccgca gtggaatgag tcctttacat 1000
tcaaattgaa accttcagac aaagaccgac gactgtctgt agaaatctgg 1050
gactgggatc gaacaacaag gaatgacttc atgggatccc tttcctttgg 1100
agtttcggag ctgatgaaga tgccggccag tggatggtac aagttgctta 1150
accaagaaga aggtgagtac tacaacgtac ccattccgga aggggacgag 1200
gaaggaaaca tggaactcag gcagaaattc gagaaagcca aacttggccc 1250
tgctggcaac agatctacta gtacgcgtat ggagaagagg gggcggattt 1300
acctaaaggc tgaggttgct gatgaaaagc tccatgtcac agtacgagat 1350
gcaaaaaatc taatccctat ggatccaaac gggctttcag atccttatgt 1400
gaagctgaaa cttattcctg atcccaagaa tgaaagcaag caaaaaacca 1450
aaaccatccg ctccacacta aatccgcagt ggaatgagtc ctttacattc 1500
aaattgaaac cttcagacaa agaccgacga ctgtctgtag aaatctggga 1550
ctgggatcga acaacaagga atgacttcat gggatccctt tcctttggag 1600
tttcggagct gatgaagatg ccggccagtg gatggtacaa gttgcttaac 1650
caagaagaag gtgagtacta caacgtaccc attccggaag gggacgagga 1700
aggaaacatg gaactcaggc agaaattcga gaaagccaaa cttggccctg 1750
ctggcaacgt cgacggtacc actagtacgc gtatgctgag ggtcttcatc 1800
ctctatgccg agaacgtcca cacacccgac accgacatca gcgatgccta 1850
ctgctccgcg gtgtttgcag gggtgaagaa gagaaccaaa gtcatcaaga 1900
acagcgtgaa ccctgtatgg aatgagggat ttgaatggga cctcaagggc 1950
atccccctgg accagggctc tgagcttcat gtggtggtca aagaccatga 2000
gacgatgggg aggaacaggt tcctggggga agccaaggtc ccactccgag 2050
aggtcctcgc cacccctagt ctgtccgcca gcttcaatgc ccccctgctg 2100
gacaccaaga agcagcccac aggggcctcg ctggtcctgc aggtgtccta 2150
cacaccgctg cctggagctg tgctggtgaa gcccttcagc ggtaccacta 2200
gtacgcgtat gctgagggtc ttcatcctct atgccgagaa cgtccacaca 2250
cccgacaccg acatcagcga tgcctactgc tccgcggtgt ttgcaggggt 2300
gaagaagaga accaaagtca tcaagaacag cgtgaaccct gtatggaatg 2350
agggatttga atgggacctc aagggcatcc ccctggacca gggctctgag 2400
cttcatgtgg tggtcaaaga ccatgagacg atggggagga acaggttcct 2450
gggggaagcc aaggtcccac tccgagaggt cctcgccacc cctagtctgt 2500
ccgccagctt caatgccccc ctgctggaca ccaagaagca gcccacaggg 2550
gcctcgctgg tcctgcaggt gtcctacaca ccgctgcctg gagctgtgct 2600
ggtgaagccc ttcagctga                                   2619

<210> 17
<211> 872
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence for pV-2xC2pkc-2xC2Astop plasmid

<400> 17
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 
1               5                   10                  15
Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val 
                20                  25                  30
Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 
                35                  40                  45
Lys Leu Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr 
                50                  55                  60
Leu Val Thr Thr Leu Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr 
                65                  70                  75
Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro 
                80                  85                  90
Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly 
                95                  100                 105
Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
                110                 115                 120
Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
                125                 130                 135
Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn 
                140                 145                 150
Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn 
                155                 160                 165
Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly Val Gln Leu Ala 
                170                 175                 180
Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
                185                 190                 195
Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser Lys 
                200                 205                 210
Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
                215                 220                 225
Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
                230                 235                 240
Gly Leu Arg Ser Arg Ala Gln Ala Ser Asn Ser Ala Val Asp Arg 
                245                 250                 255
Ser Thr Ser Thr Arg Met Glu Lys Arg Gly Arg Ile Tyr Leu Lys 
                260                 265                 270
Ala Glu Val Ala Asp Glu Lys Leu His Val Thr Val Arg Asp Ala 
                275                 280                 285
Lys Asn Leu Ile Pro Met Asp Pro Asn Gly Leu Ser Asp Pro Tyr 
                290                 295                 300
Val Lys Leu Lys Leu Ile Pro Asp Pro Lys Asn Glu Ser Lys Gln 
                305                 310                 315
Lys Thr Lys Thr Ile Arg Ser Thr Leu Asn Pro Gln Trp Asn Glu 
                320                 325                 330
Ser Phe Thr Phe Lys Leu Lys Pro Ser Asp Lys Asp Arg Arg Leu 
                335                 340                 345
Ser Val Glu Ile Trp Asp Trp Asp Arg Thr Thr Arg Asn Asp Phe 
                350                 355                 360
Met Gly Ser Leu Ser Phe Gly Val Ser Glu Leu Met Lys Met Pro 
                365                 370                 375
Ala Ser Gly Trp Tyr Lys Leu Leu Asn Gln Glu Glu Gly Glu Tyr 
                380                 385                 390
Tyr Asn Val Pro Ile Pro Glu Gly Asp Glu Glu Gly Asn Met Glu 
                395                 400                 405
Leu Arg Gln Lys Phe Glu Lys Ala Lys Leu Gly Pro Ala Gly Asn 
                410                 415                 420
Arg Ser Thr Ser Thr Arg Met Glu Lys Arg Gly Arg Ile Tyr Leu 
                425                 430                 435
Lys Ala Glu Val Ala Asp Glu Lys Leu His Val Thr Val Arg Asp 
                440                 445                 450
Ala Lys Asn Leu Ile Pro Met Asp Pro Asn Gly Leu Ser Asp Pro 
                455                 460                 465
Tyr Val Lys Leu Lys Leu Ile Pro Asp Pro Lys Asn Glu Ser Lys 
                470                 475                 480
Gln Lys Thr Lys Thr Ile Arg Ser Thr Leu Asn Pro Gln Trp Asn 
                485                 490                 495
Glu Ser Phe Thr Phe Lys Leu Lys Pro Ser Asp Lys Asp Arg Arg 
                500                 505                 510
Leu Ser Val Glu Ile Trp Asp Trp Asp Arg Thr Thr Arg Asn Asp 
                515                 520                 525
Phe Met Gly Ser Leu Ser Phe Gly Val Ser Glu Leu Met Lys Met 
                530                 535                 540
Pro Ala Ser Gly Trp Tyr Lys Leu Leu Asn Gln Glu Glu Gly Glu 
                545                 550                 555
Tyr Tyr Asn Val Pro Ile Pro Glu Gly Asp Glu Glu Gly Asn Met 
                560                 565                 570
Glu Leu Arg Gln Lys Phe Glu Lys Ala Lys Leu Gly Pro Ala Gly 
                575                 580                 585
Asn Val Asp Gly Thr Thr Ser Thr Arg Met Leu Arg Val Phe Ile 
                590                 595                 600
Leu Tyr Ala Glu Asn Val His Thr Pro Asp Thr Asp Ile Ser Asp 
                605                 610                 615
Ala Tyr Cys Ser Ala Val Phe Ala Gly Val Lys Lys Arg Thr Lys 
                620                 625                 630
Val Ile Lys Asn Ser Val Asn Pro Val Trp Asn Glu Gly Phe Glu 
                635                 640                 645
Trp Asp Leu Lys Gly Ile Pro Leu Asp Gln Gly Ser Glu Leu His 
                650                 655                 660
Val Val Val Lys Asp His Glu Thr Met Gly Arg Asn Arg Phe Leu 
                665                 670                 675
Gly Glu Ala Lys Val Pro Leu Arg Glu Val Leu Ala Thr Pro Ser 
                680                 685                 690
Leu Ser Ala Ser Phe Asn Ala Pro Leu Leu Asp Thr Lys Lys Gln 
                695                 700                 705
Pro Thr Gly Ala Ser Leu Val Leu Gln Val Ser Tyr Thr Pro Leu 
                710                 715                 720
Pro Gly Ala Val Leu Val Lys Pro Phe Ser Gly Thr Thr Ser Thr 
                725                 730                 735
Arg Met Leu Arg Val Phe Ile Leu Tyr Ala Glu Asn Val His Thr 
                740                 745                 750
Pro Asp Thr Asp Ile Ser Asp Ala Tyr Cys Ser Ala Val Phe Ala 
                755                 760                 765
Gly Val Lys Lys Arg Thr Lys Val Ile Lys Asn Ser Val Asn Pro 
                770                 775                 780
Val Trp Asn Glu Gly Phe Glu Trp Asp Leu Lys Gly Ile Pro Leu 
                785                 790                 795
Asp Gln Gly Ser Glu Leu His Val Val Val Lys Asp His Glu Thr 
                800                 805                 810
Met Gly Arg Asn Arg Phe Leu Gly Glu Ala Lys Val Pro Leu Arg 
                815                 820                 825
Glu Val Leu Ala Thr Pro Ser Leu Ser Ala Ser Phe Asn Ala Pro 
                830                 835                 840
Leu Leu Asp Thr Lys Lys Gln Pro Thr Gly Ala Ser Leu Val Leu 
                845                 850                 855
Gln Val Ser Tyr Thr Pro Leu Pro Gly Ala Val Leu Val Lys Pro 
                860                 865                 870
Phe Ser