                         SEQUENCE LISTING

<110>  Toleranzia AB
 
<120>  FUSION PROTEIN IN MYASTHENIA GRAVIS

<130>  HSJ122772P.WOP

<150>  US 63/197,722
<151>  2021-06-07

<150>  SE 2150726-4
<151>  2021-06-09

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  482
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Glu Pro Trp Pro Leu Leu Leu Leu Phe Ser Leu Cys Ser Ala Gly 
1               5                   10                  15      


Leu Val Leu Gly Ser Glu His Glu Thr Arg Leu Val Ala Lys Leu Phe 
            20                  25                  30          


Lys Asp Tyr Ser Ser Val Val Arg Pro Val Glu Asp His Arg Gln Val 
        35                  40                  45              


Val Glu Val Thr Val Gly Leu Gln Leu Ile Gln Leu Ile Asn Val Asp 
    50                  55                  60                  


Glu Val Asn Gln Ile Val Thr Thr Asn Val Arg Leu Lys Gln Gly Asp 
65                  70                  75                  80  


Met Val Asp Leu Pro Arg Pro Ser Cys Val Thr Leu Gly Val Pro Leu 
                85                  90                  95      


Phe Ser His Leu Gln Asn Glu Gln Trp Val Asp Tyr Asn Leu Lys Trp 
            100                 105                 110         


Asn Pro Asp Asp Tyr Gly Gly Val Lys Lys Ile His Ile Pro Ser Glu 
        115                 120                 125             


Lys Ile Trp Arg Pro Asp Leu Val Leu Tyr Asn Asn Ala Asp Gly Asp 
    130                 135                 140                 


Phe Ala Ile Val Lys Phe Thr Lys Val Leu Leu Gln Tyr Thr Gly His 
145                 150                 155                 160 


Ile Thr Trp Thr Pro Pro Ala Ile Phe Lys Ser Tyr Cys Glu Ile Ile 
                165                 170                 175     


Val Thr His Phe Pro Phe Asp Glu Gln Asn Cys Ser Met Lys Leu Gly 
            180                 185                 190         


Thr Trp Thr Tyr Asp Gly Ser Val Val Ala Ile Asn Pro Glu Ser Asp 
        195                 200                 205             


Gln Pro Asp Leu Ser Asn Phe Met Glu Ser Gly Glu Trp Val Ile Lys 
    210                 215                 220                 


Glu Ser Arg Gly Trp Lys His Ser Val Thr Tyr Ser Cys Cys Pro Asp 
225                 230                 235                 240 


Thr Pro Tyr Leu Asp Ile Thr Tyr His Phe Val Met Gln Arg Leu Pro 
                245                 250                 255     


Leu Tyr Phe Ile Val Asn Val Ile Ile Pro Cys Leu Leu Phe Ser Phe 
            260                 265                 270         


Leu Thr Gly Leu Val Phe Tyr Leu Pro Thr Asp Ser Gly Glu Lys Met 
        275                 280                 285             


Thr Leu Ser Ile Ser Val Leu Leu Ser Leu Thr Val Phe Leu Leu Val 
    290                 295                 300                 


Ile Val Glu Leu Ile Pro Ser Thr Ser Ser Ala Val Pro Leu Ile Gly 
305                 310                 315                 320 


Lys Tyr Met Leu Phe Thr Met Val Phe Val Ile Ala Ser Ile Ile Ile 
                325                 330                 335     


Thr Val Ile Val Ile Asn Thr His His Arg Ser Pro Ser Thr His Val 
            340                 345                 350         


Met Pro Asn Trp Val Arg Lys Val Phe Ile Asp Thr Ile Pro Asn Ile 
        355                 360                 365             


Met Phe Phe Ser Thr Met Lys Arg Pro Ser Arg Glu Lys Gln Asp Lys 
    370                 375                 380                 


Lys Ile Phe Thr Glu Asp Ile Asp Ile Ser Asp Ile Ser Gly Lys Pro 
385                 390                 395                 400 


Gly Pro Pro Pro Met Gly Phe His Ser Pro Leu Ile Lys His Pro Glu 
                405                 410                 415     


Val Lys Ser Ala Ile Glu Gly Ile Lys Tyr Ile Ala Glu Thr Met Lys 
            420                 425                 430         


Ser Asp Gln Glu Ser Asn Asn Ala Ala Ala Glu Trp Lys Tyr Val Ala 
        435                 440                 445             


Met Val Met Asp His Ile Leu Leu Gly Val Phe Met Leu Val Cys Ile 
    450                 455                 460                 


Ile Gly Thr Leu Ala Val Phe Ala Gly Arg Leu Ile Glu Leu Asn Gln 
465                 470                 475                 480 


Gln Gly 
        


<210>  2
<211>  235
<212>  PRT
<213>  Homo sapiens

<400>  2

Ser Glu His Glu Thr Arg Leu Val Ala Lys Leu Phe Lys Asp Tyr Ser 
1               5                   10                  15      


Ser Val Val Arg Pro Val Glu Asp His Arg Gln Val Val Glu Val Thr 
            20                  25                  30          


Val Gly Leu Gln Leu Ile Gln Leu Ile Asn Val Asp Glu Val Asn Gln 
        35                  40                  45              


Ile Val Thr Thr Asn Val Arg Leu Lys Gln Gly Asp Met Val Asp Leu 
    50                  55                  60                  


Pro Arg Pro Ser Cys Val Thr Leu Gly Val Pro Leu Phe Ser His Leu 
65                  70                  75                  80  


Gln Asn Glu Gln Trp Val Asp Tyr Asn Leu Lys Trp Asn Pro Asp Asp 
                85                  90                  95      


Tyr Gly Gly Val Lys Lys Ile His Ile Pro Ser Glu Lys Ile Trp Arg 
            100                 105                 110         


Pro Asp Leu Val Leu Tyr Asn Asn Ala Asp Gly Asp Phe Ala Ile Val 
        115                 120                 125             


Lys Phe Thr Lys Val Leu Leu Gln Tyr Thr Gly His Ile Thr Trp Thr 
    130                 135                 140                 


Pro Pro Ala Ile Phe Lys Ser Tyr Cys Glu Ile Ile Val Thr His Phe 
145                 150                 155                 160 


Pro Phe Asp Glu Gln Asn Cys Ser Met Lys Leu Gly Thr Trp Thr Tyr 
                165                 170                 175     


Asp Gly Ser Val Val Ala Ile Asn Pro Glu Ser Asp Gln Pro Asp Leu 
            180                 185                 190         


Ser Asn Phe Met Glu Ser Gly Glu Trp Val Ile Lys Glu Ser Arg Gly 
        195                 200                 205             


Trp Lys His Ser Val Thr Tyr Ser Cys Cys Pro Asp Thr Pro Tyr Leu 
    210                 215                 220                 


Asp Ile Thr Tyr His Phe Val Met Gln Arg Leu 
225                 230                 235 


<210>  3
<211>  210
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified extracellular domain of nAChRalpha1

<400>  3

Ser Glu His Glu Thr Arg Leu Val Ala Lys Leu Phe Lys Asp Tyr Ser 
1               5                   10                  15      


Ser Val Val Arg Pro Val Glu Asp His Arg Gln Val Val Glu Val Thr 
            20                  25                  30          


Val Gly Leu Gln Leu Ile Gln Leu Ile Asn Val Asp Glu Val Asn Gln 
        35                  40                  45              


Ile Val Thr Thr Asn Val Arg Leu Lys Gln Gln Trp Val Asp Tyr Asn 
    50                  55                  60                  


Leu Lys Trp Asn Pro Asp Asp Tyr Gly Gly Val Lys Lys Ile His Ile 
65                  70                  75                  80  


Pro Ser Glu Lys Ile Trp Arg Pro Asp Leu Val Leu Tyr Asn Asn Ala 
                85                  90                  95      


Asp Gly Asp Phe Ala Ile Val Lys Phe Thr Lys Val Leu Leu Gln Tyr 
            100                 105                 110         


Thr Gly His Ile Thr Trp Thr Pro Pro Ala Ile Phe Lys Ser Tyr Cys 
        115                 120                 125             


Glu Ile Ile Val Thr His Phe Pro Phe Asp Glu Gln Asn Cys Ser Met 
    130                 135                 140                 


Lys Leu Gly Thr Trp Thr Tyr Asp Gly Ser Val Val Ala Ile Asn Pro 
145                 150                 155                 160 


Glu Ser Asp Gln Pro Asp Leu Ser Asn Phe Met Glu Ser Gly Glu Trp 
                165                 170                 175     


Val Ile Lys Glu Ser Arg Gly Trp Lys His Ser Val Thr Tyr Ser Cys 
            180                 185                 190         


Cys Pro Asp Thr Pro Tyr Leu Asp Ile Thr Tyr His Phe Val Met Gln 
        195                 200                 205             


Arg Leu 
    210 


<210>  4
<211>  12
<212>  PRT
<213>  Lymnaea stagnalis

<400>  4

Asp Val Ser Gly Val Asp Thr Glu Ser Gly Ala Thr 
1               5                   10          


<210>  5
<211>  210
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TOL2 fusion protein

<400>  5

Ser Glu His Glu Thr Arg Leu Val Ala Lys Leu Phe Lys Asp Tyr Ser 
1               5                   10                  15      


Ser Val Val Arg Pro Val Glu Asp His Arg Gln Val Val Glu Val Thr 
            20                  25                  30          


Val Gly Leu Gln Leu Ile Gln Leu Ile Asn Val Asp Glu Val Asn Gln 
        35                  40                  45              


Ile Val Thr Thr Asn Val Arg Leu Lys Gln Gln Trp Val Asp Tyr Asn 
    50                  55                  60                  


Leu Lys Trp Asn Pro Asp Asp Tyr Gly Gly Val Lys Lys Ile His Ile 
65                  70                  75                  80  


Pro Ser Glu Lys Ile Trp Arg Pro Asp Leu Val Leu Tyr Asn Asn Ala 
                85                  90                  95      


Asp Gly Asp Phe Ala Ile Val Lys Phe Thr Lys Val Leu Leu Gln Tyr 
            100                 105                 110         


Thr Gly His Ile Thr Trp Thr Pro Pro Ala Ile Phe Lys Ser Tyr Cys 
        115                 120                 125             


Asp Val Ser Gly Val Asp Thr Glu Ser Gly Ala Thr Asn Cys Ser Met 
    130                 135                 140                 


Lys Leu Gly Thr Trp Thr Tyr Asp Gly Ser Val Val Ala Ile Asn Pro 
145                 150                 155                 160 


Glu Ser Asp Gln Pro Asp Leu Ser Asn Phe Met Glu Ser Gly Glu Trp 
                165                 170                 175     


Val Ile Lys Glu Ser Arg Gly Trp Lys His Ser Val Thr Tyr Ser Cys 
            180                 185                 190         


Cys Pro Asp Thr Pro Tyr Leu Asp Ile Thr Tyr His Phe Val Met Gln 
        195                 200                 205             


Arg Leu 
    210 


<210>  6
<211>  211
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TOL2 fusion protein

<400>  6

Met Ser Glu His Glu Thr Arg Leu Val Ala Lys Leu Phe Lys Asp Tyr 
1               5                   10                  15      


Ser Ser Val Val Arg Pro Val Glu Asp His Arg Gln Val Val Glu Val 
            20                  25                  30          


Thr Val Gly Leu Gln Leu Ile Gln Leu Ile Asn Val Asp Glu Val Asn 
        35                  40                  45              


Gln Ile Val Thr Thr Asn Val Arg Leu Lys Gln Gln Trp Val Asp Tyr 
    50                  55                  60                  


Asn Leu Lys Trp Asn Pro Asp Asp Tyr Gly Gly Val Lys Lys Ile His 
65                  70                  75                  80  


Ile Pro Ser Glu Lys Ile Trp Arg Pro Asp Leu Val Leu Tyr Asn Asn 
                85                  90                  95      


Ala Asp Gly Asp Phe Ala Ile Val Lys Phe Thr Lys Val Leu Leu Gln 
            100                 105                 110         


Tyr Thr Gly His Ile Thr Trp Thr Pro Pro Ala Ile Phe Lys Ser Tyr 
        115                 120                 125             


Cys Asp Val Ser Gly Val Asp Thr Glu Ser Gly Ala Thr Asn Cys Ser 
    130                 135                 140                 


Met Lys Leu Gly Thr Trp Thr Tyr Asp Gly Ser Val Val Ala Ile Asn 
145                 150                 155                 160 


Pro Glu Ser Asp Gln Pro Asp Leu Ser Asn Phe Met Glu Ser Gly Glu 
                165                 170                 175     


Trp Val Ile Lys Glu Ser Arg Gly Trp Lys His Ser Val Thr Tyr Ser 
            180                 185                 190         


Cys Cys Pro Asp Thr Pro Tyr Leu Asp Ile Thr Tyr His Phe Val Met 
        195                 200                 205             


Gln Arg Leu 
    210     


<210>  7
<211>  654
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TOL2 fusion protein coding sequence

<400>  7
ggtctcccca tgagcgaaca tgaaacccgt ctggttgcaa aactgttcaa agattatagc       60

agcgttgttc gtccggttga agatcatcgt caggttgttg aagttaccgt tggtctgcag      120

ctgatccagc tgattaatgt tgatgaagtg aatcagattg tgaccaccaa tgttcgtctg      180

aaacagcagt gggttgatta taacctgaaa tggaatccgg atgattatgg tggcgtgaaa      240

aaaatccata ttccgagcga aaaaatctgg cgtccggatc tggttctgta taataacgca      300

gatggtgatt ttgccatcgt gaaatttacc aaagttctgc tgcagtatac cggtcatatt      360

acctggacac cgcctgcaat ctttaaaagc tattgtgatg ttagcggtgt ggataccgaa      420

agcggtgcaa ccaattgtag catgaaatta ggcacctgga cctatgatgg tagcgttgtt      480

gcaattaatc cggaaagcga tcagccggat ctgagcaatt tcatggaaag tggtgaatgg      540

gtgattaaag aaagccgtgg ttggaaacat agcgttacct atagctgttg tccggataca      600

ccgtatctgg atattaccta tcattttgtt atgcagcgcc tgtaagccga gacc            654


