               SEQUENCE LISTING

<110> Bayer AG

<120> BNovel Type V RNA programmable endonuclease systems

<130> BHC211037-FC

<160> 44

<170> BiSSAP 1.3.6

<210> 1
<211> 1103
<212> PRT
<213> other sequences


<220> 
<223> RNA programmable DNA endonuclease B-Gen.1

<400> 1
Met Thr Ile Arg Ser Met Lys Leu Lys Leu Lys Ile Tyr Ser Gly Arg 
1               5                   10                  15      
Ser Ala Pro Gln Leu Arg Gln Gly Leu Trp Arg Leu His Arg Leu Leu 
            20                  25                  30          
Asn Glu Gly Thr Ala Tyr Tyr Met Asp Trp Leu Val His Met Arg Gln 
        35                  40                  45              
Glu Ala Leu Pro Gly Lys Ser Lys Glu Glu Ile Arg Ala Glu Leu Glu 
    50                  55                  60                  
Arg Arg Val Arg Gln Gln Gln Glu Lys Asn Gly Val Gln Asn Asp Gln 
65                  70                  75                  80  
Val Pro Met Asp Glu Val Leu Ser Ala Leu Arg Gln Leu Tyr Glu Leu 
                85                  90                  95      
Leu Val Pro Ser Ala Val Asn Asn Ser Gly Asp Ala Gln Thr Leu Ser 
            100                 105                 110         
Arg Lys Phe Leu Ser Pro Leu Val Asp Pro Asn Ser Glu Gly Gly Lys 
        115                 120                 125             
Gly Thr Ser Asn Ala Gly Ala Lys Pro Gly Trp Arg Lys Lys Gln Glu 
    130                 135                 140                 
Ala Gly Asp Pro Ser Trp Glu Lys Asp Tyr Glu Arg Trp Leu Lys Arg 
145                 150                 155                 160 
Lys Gln Ala Asp Pro Thr Ala Glu Ile Leu Gly Lys Leu Glu Thr Ala 
                165                 170                 175     
Gly Leu Lys Pro Leu Phe Pro Leu Tyr Thr Asn Glu Val Lys Asp Ile 
            180                 185                 190         
Arg Trp Met Pro Leu Thr Ser Lys Gln Tyr Val Arg Asn Trp Asp Arg 
        195                 200                 205             
Asp Met Phe Gln Gln Ala Ile Glu His Leu Leu Ser Trp Glu Thr Trp 
    210                 215                 220                 
Asn Arg Lys Val Asn Glu Glu Arg Ala Lys Leu Lys Glu Thr Val Arg 
225                 230                 235                 240 
Arg Phe Glu Glu Gln His Leu Ala Asn Gly Lys Asp Trp Leu Ser Pro 
                245                 250                 255     
Leu Gln Ala Tyr Glu Ala Asn Arg Glu Gln Ala Leu Arg Asp Met Ala 
            260                 265                 270         
Ile Ser Pro Ser Asp Arg Phe Arg Ile Thr Arg Arg Gln Ile Lys Gly 
        275                 280                 285             
Trp Ser Glu Leu Tyr Glu Arg Trp Asn Lys Leu Ala Pro Thr Ala Ser 
    290                 295                 300                 
Val Glu Ala Tyr Met Gln Glu Val Arg His Val Gln Lys Lys Leu Gly 
305                 310                 315                 320 
Gly Thr Phe Gly Asp Ala Asp Leu Tyr Arg Phe Leu Ala Lys Pro Glu 
                325                 330                 335     
Asn Val His Ile Trp Arg Asp His Gln Glu Arg Leu His Tyr Tyr Ala 
            340                 345                 350         
Ala Tyr Asn Asp Leu His Lys Arg Leu Met Ser Ala Lys Glu Gln Ala 
        355                 360                 365             
Ala Phe Thr Leu Pro Asp Pro Val Ala His Pro Leu Trp Val Arg Phe 
    370                 375                 380                 
Asp Ala Arg Asp Gly Asn Leu Phe Thr Tyr Ile Leu Gln Ala Asp Ser 
385                 390                 395                 400 
Ser Lys Gln Arg Ser Arg Arg Tyr Val Asn Phe Ser Arg Phe Leu Trp 
                405                 410                 415     
Pro Val Glu Asp Gly Tyr Phe Glu Glu Thr Glu Asn Val Lys Val Glu 
            420                 425                 430         
Leu Ala Leu Ser Lys Gln Phe Tyr Arg Gln Val Ile Val His Asp Asn 
        435                 440                 445             
Pro Thr Gly Lys Gln Lys Ile Thr Phe Gln Asp Tyr Ser Ser Lys Glu 
    450                 455                 460                 
Ile Leu Glu Gly His Leu Gly Gly Ala Lys Leu Gln Leu Asp Arg Asn 
465                 470                 475                 480 
Phe Leu Arg Lys Ser Gly Arg Asp Phe Glu Thr Gly Asp Phe Gly Pro 
                485                 490                 495     
Ala Phe Leu Asn Val Val Leu Asp Leu Lys Pro Lys Gln Glu Val Lys 
            500                 505                 510         
Asn Gly Arg Leu Gln Ser Pro Leu Gly Gln Ala Leu Leu Val Lys Ser 
        515                 520                 525             
Arg Pro Asn Asp Ile Pro Lys Val Tyr Gly Tyr Lys Pro Asp Ala Leu 
    530                 535                 540                 
Ala Ala Trp Leu Glu Gln Ala Ser Gly Glu Glu Ser Leu Gly Ser Glu 
545                 550                 555                 560 
Ser Leu Arg Gln Gly Phe Arg Val Met Ser Ile Asp Leu Gly Val Arg 
                565                 570                 575     
Ser Ala Ala Ala Ile Ser Val Phe Ser Val Lys Gly Glu Lys Thr Arg 
            580                 585                 590         
Glu Gly Asp Lys Val Cys Tyr Pro Val Gly Glu Thr Gly Leu Phe Ala 
        595                 600                 605             
Val His Asp Arg Ser Phe Leu Leu Arg Leu Pro Gly Glu Ser Ser Glu 
    610                 615                 620                 
Lys Arg Val Asn Val Glu Arg Asp Lys Arg Lys Thr Glu Arg Met Gln 
625                 630                 635                 640 
Ile Arg Tyr His Ile Arg Thr Leu Ala Arg Val Leu Arg Leu Ala Asn 
                645                 650                 655     
Lys Ala Thr Pro Met Asp Arg Ile Lys Ala Val Gln Asp Val Leu Asn 
            660                 665                 670         
Asp Ile Glu Ser Thr Arg Phe Met Asn Asp His Asp His His Val Tyr 
        675                 680                 685             
Asn His Ala Leu Glu Thr Leu Arg Thr Tyr Ala Pro Asp His Gln Gly 
    690                 695                 700                 
Ile Trp Glu Glu Gln Val Ile Ala Ala His Arg Gln Leu Glu His His 
705                 710                 715                 720 
Val Gly Val Ile Val Gly Glu Trp Arg Lys Asn Trp Gly Lys Asp Arg 
                725                 730                 735     
Arg Gly Thr Val Gly Leu Ser Met Asp Asn Ile Glu Glu Leu Asp Glu 
            740                 745                 750         
Met Arg Arg Leu Leu Ile Ser Trp Ser Arg Arg Ala Arg Tyr Pro Arg 
        755                 760                 765             
Glu Ala Lys Pro Phe Gln Val Asn Glu Ser Asn Pro Val His Leu Leu 
    770                 775                 780                 
Arg His Leu Gln Asn Leu Lys Glu Asp Arg Leu Lys Gln Leu Ala Asn 
785                 790                 795                 800 
Leu Ile Val Met Thr Ala Leu Gly Tyr Val Tyr Asp Ser Lys Glu Lys 
                805                 810                 815     
Lys Trp Lys Ala Ala Tyr Pro Ala Cys Gln Leu Ile Leu Phe Glu Asp 
            820                 825                 830         
Leu Gln Arg Tyr Arg Phe His Leu Asp Arg Ser Ala Arg Glu Asn Ser 
        835                 840                 845             
Gln Leu Met Lys Trp Ala His Arg Ser Ile Pro Lys Tyr Val Trp Met 
    850                 855                 860                 
Gln Gly Glu Pro Tyr Gly Leu Gln Ile Gly Asp Val Trp Ala Gly Phe 
865                 870                 875                 880 
Thr Ser Arg Tyr His Ala Lys Thr Gly Ala Pro Gly Ile Arg Cys Lys 
                885                 890                 895     
Ala Leu Thr Glu Lys Asp Phe Gln Gln Gly Arg Leu Leu Glu Ser Leu 
            900                 905                 910         
Val Ala Glu Gly Met Phe Thr Leu Gln Glu Val Gly Thr Leu Lys Pro 
        915                 920                 925             
Gly Asp Ile Val Pro Ala Glu Gly Gly Glu Leu Phe Val Thr Leu Ala 
    930                 935                 940                 
Asp Asp Ser Gly Asp Arg Ile Val Ile Thr His Ala Asp Ile Asn Ala 
945                 950                 955                 960 
Ala Gln Asn Val Gln Lys Arg Phe Trp Leu Ala Asn Ser Glu Arg Phe 
                965                 970                 975     
Arg Val Ala Cys Arg Ser Val Gln Ile Ala Ser Gln Glu Cys Phe Ile 
            980                 985                 990         
Pro Ser Ser Glu Ser Val Ala Lys Lys Met Gly Lys Gly Val Phe Val 
        995                 1000                1005            
Arg Asp Phe Ser Phe His Lys Asp Met Glu Val Tyr His Trp Asn Asn 
    1010                1015                1020                
Gln Val Lys Leu Thr Ala Lys Asn Val Pro Thr Asp His Ser Asp Asp 
1025                1030                1035                1040
Leu Gln Asp Leu Gln Asp Tyr Gln Ala Ile Leu Glu Glu Ala Arg Glu 
                1045                1050                1055    
Ser Ser Ser Ser Tyr Lys Thr Leu Phe Arg Asp Pro Ser Gly Phe Phe 
            1060                1065                1070        
Phe Pro Asp Asp Val Trp Val Pro Gln Asn Ile Tyr Trp Arg Glu Val 
        1075                1080                1085            
Lys Lys Thr Ile Thr Ala Leu Leu Arg Lys Arg Ile Met Ser Thr 
    1090                1095                1100            

<210> 2
<211> 1091
<212> PRT
<213> other sequences


<220> 
<223> RNA programmable DNA endonuclease B-Gen.2

<400> 2
Met Pro Ile Arg Ser Phe Lys Leu Lys Leu Val Thr His Asn Gly Asp 
1               5                   10                  15      
Ser Thr Tyr Met Asp Lys Leu Arg Arg Gly Leu Trp Lys Thr His Val 
            20                  25                  30          
Ile Ile Asn Arg Gly Ile Ala Tyr Tyr Met Asn Thr Leu Ala Leu Met 
        35                  40                  45              
Arg Gln Glu Pro Tyr Gly Ser Lys Ser Arg Glu Glu Val Arg Leu Asp 
    50                  55                  60                  
Leu Leu Ser Thr Leu Arg Glu Gln Gln Arg Arg Asn Asn Trp Ser Glu 
65                  70                  75                  80  
Gln Thr Gly Thr Asp Asp Glu Leu Leu Ser Leu Ser Arg Arg Val Tyr 
                85                  90                  95      
Glu Leu Leu Val Pro Ser Ala Ile Gly Glu Lys Gly Asp Ala Gln Met 
            100                 105                 110         
Leu Ser Arg Lys Phe Leu Ser Pro Leu Val Asp Pro Asn Ser Glu Gly 
        115                 120                 125             
Gly Arg Gly Thr Ala Lys Ser Gly Arg Lys Pro Arg Trp Lys Lys Met 
    130                 135                 140                 
Met Glu Glu Gly His Pro Asp Trp Glu Lys Glu Lys Glu Lys Asp Ala 
145                 150                 155                 160 
Ala Lys Lys Ala Glu Asp Pro Thr Ala Ser Ile Leu Ala Asp Leu Glu 
                165                 170                 175     
Ala Val Gly Leu Leu Pro Leu Phe Pro Leu Phe Ser Asp Glu Gln Lys 
            180                 185                 190         
Glu Ile Arg Trp Leu Pro Lys Lys Lys Arg Gln Phe Val Arg Thr Trp 
        195                 200                 205             
Asp Arg Asp Met Phe Gln Gln Ala Leu Glu Arg Met Leu Ser Trp Glu 
    210                 215                 220                 
Ser Trp Asn Arg Arg Val Ala Glu Glu Tyr Leu Lys Leu Gln Ala Gln 
225                 230                 235                 240 
Arg Asp Glu Val Tyr Ala Lys Tyr Leu Glu Asp Ala Gly Ser Trp Leu 
                245                 250                 255     
Asn Asp Leu Gln Thr Phe Glu Lys Gln Arg Glu Glu Glu Leu Ala Glu 
            260                 265                 270         
Val Ser Phe Glu Pro Asn Ser Glu Tyr Leu Ile Thr Arg Arg Gln Ile 
        275                 280                 285             
Arg Gly Trp Lys Glu Val Tyr Glu Lys Trp Ser Lys Thr Ser Glu Asn 
    290                 295                 300                 
Ala Ser Gln Glu Gln Leu Trp Arg Met Val Ala Asp Val Gln Thr Ala 
305                 310                 315                 320 
Met Ala Gly Ala Phe Gly Asp Pro Lys Val Tyr Gln Phe Leu Ser Gln 
                325                 330                 335     
Pro Lys His His His Ile Trp Arg Glu His Pro Asn Arg Leu Phe Tyr 
            340                 345                 350         
Tyr Ser Lys Tyr Asn Glu Val Arg Glu Lys Leu Asn Arg Ala Lys Lys 
        355                 360                 365             
Gln Ala Ala Phe Thr Leu Pro Asp Pro Val Glu His Pro Leu Trp Thr 
    370                 375                 380                 
Arg Phe Asp Ala Arg Gly Gly Asn Ile His Asp Tyr Glu Ile Ser Lys 
385                 390                 395                 400 
Val Gly Lys Gln Tyr His Val Thr Phe Ser Ser Leu Ile Leu Pro Glu 
                405                 410                 415     
Ala Gln Ser Trp Val Glu Ile Glu Asn Val Thr Val Gly Ile Gly Asn 
            420                 425                 430         
Ser Leu Gln Leu Lys Arg Gln Ile Arg Leu Asp Gly Tyr Ala Asp Lys 
        435                 440                 445             
Lys Gln Lys Val Lys Tyr Tyr Asp Tyr Ser Ser Arg Phe Glu Leu Thr 
    450                 455                 460                 
Gly Val Leu Gly Gly Ala Lys Ile Gln Phe Asp Arg Lys His Leu Lys 
465                 470                 475                 480 
Lys Ala Ala His Arg Leu Ala Glu Gly Glu Thr Gly Pro Ile Phe Leu 
                485                 490                 495     
Asn Val Val Val Asp Val Glu Pro Phe Leu Glu Val Lys Asn Gly Arg 
            500                 505                 510         
Leu Arg Thr Pro Leu Gly Gln Val Leu Gln Val Asn Thr Arg Asp Trp 
        515                 520                 525             
Pro Lys Val Val Asp Tyr Lys Ala Lys Glu Leu Ser Val Leu Met Glu 
    530                 535                 540                 
Asn Thr Gln Ile Gly Asn Glu Asn Gly Val Ser Thr Ile Glu Ala Gly 
545                 550                 555                 560 
Met Arg Ile Met Ser Ile Asp Leu Gly Gln Arg Thr Ala Ala Ala Val 
                565                 570                 575     
Ser Ile Phe Glu Val Ile Ser Lys Lys Pro Asp Glu Lys Glu Thr Lys 
            580                 585                 590         
Leu Phe Tyr Pro Ile Ala Asp Thr Asp Leu Tyr Ala Val His Arg Arg 
        595                 600                 605             
Ser Leu Leu Leu Arg Leu Pro Gly Glu Glu Ile Ser Ser Lys Lys Met 
    610                 615                 620                 
Ile Glu Lys Arg Lys Glu Arg Ala Arg Ile Arg Ser Leu Val Arg Tyr 
625                 630                 635                 640 
Gln Ile Arg Leu Leu Ser Glu Val Leu Arg Leu His Thr Gln Gly Thr 
                645                 650                 655     
Ala Glu Gln Arg Arg Phe Lys Leu Asp Glu Leu Leu Val Ser Ile Gln 
            660                 665                 670         
Lys Lys Leu Glu Leu Asp Gln Ser Glu Trp Ile Ser Glu Leu Glu Lys 
        675                 680                 685             
Leu Phe Asp Tyr Ile Asp Glu Ser Ala Glu Lys Trp Lys Glu Ala Leu 
    690                 695                 700                 
Val Val Ala His Arg Thr Leu Glu Pro Ile Val Val Glu Ala Val Arg 
705                 710                 715                 720 
Asn Trp Lys Lys Ser Leu Ser Lys Glu Asn Lys Asp Arg Arg Arg Ile 
                725                 730                 735     
Ala Gly Ile Ser Ile Trp Ser Ile Glu Glu Leu Glu Glu Thr Arg Lys 
            740                 745                 750         
Leu Leu Ile Ala Trp Ser Lys His Ser Arg Glu Pro Gly Ile Pro Lys 
        755                 760                 765             
Arg Leu Glu Lys Glu Glu Thr Phe Ala Pro Glu His Leu Gln His Ile 
    770                 775                 780                 
Gln Asn Val Lys Asp Asp Arg Leu Lys Gln Met Ala Asn Leu Phe Val 
785                 790                 795                 800 
Met Thr Ala Leu Gly Tyr Lys Tyr Asp Glu Gly Asn Lys Arg Trp Val 
                805                 810                 815     
Glu Ala Tyr Pro Ala Cys Gln Val Ile Leu Phe Glu Asp Leu Ser Arg 
            820                 825                 830         
Tyr Arg Phe Ala Leu Asp Arg Pro Arg Arg Glu Asn Asn Arg Leu Met 
        835                 840                 845             
Lys Trp Ala His Arg Ser Ile Pro Arg Leu Thr Tyr Met Gln Ala Glu 
    850                 855                 860                 
Leu Phe Gly Ile Gln Val Gly Asp Val Tyr Ser Ala Tyr Thr Ser Arg 
865                 870                 875                 880 
Phe His Ala Lys Thr Gly Ala Pro Gly Ile Arg Cys His Ala Leu Thr 
                885                 890                 895     
Glu Ala Asp Leu Gln Ser Asn Ser Tyr Val Val Asn Gln Leu Ile Lys 
            900                 905                 910         
Asp Lys Phe Ile Gln Asp Asn Gln Thr Glu Ile Leu Lys Ala Gly Gln 
        915                 920                 925             
Ile Val Pro Trp Gln Gly Gly Glu Leu Phe Val Thr Phe Ala Asp Arg 
    930                 935                 940                 
Ser Gly Ala Ser Leu Ala Val Ile His Ala Asp Ile Asn Ala Ala Gln 
945                 950                 955                 960 
Asn Leu Gln Lys Arg Phe Trp Gln His Asn Ser Glu Val Phe Arg Val 
                965                 970                 975     
Pro Cys Lys Val Val Lys Gly Gly Leu Val Pro Val Tyr Glu Lys Met 
            980                 985                 990         
Arg Lys Leu Phe Gly Lys Gly Leu Phe Val Asn Ile Asp Asp Pro Glu 
        995                 1000                1005            
Ser Lys Glu Val Tyr Arg Trp Glu His Ser Thr Lys Met Lys Ser Lys 
    1010                1015                1020                
Thr Thr Pro Val Asp Leu Glu Ser Glu Asp Ile Asp His Glu Glu Leu 
1025                1030                1035                1040
Ser Asp Glu Trp Glu Asp Met Gln Glu Gly Tyr Lys Thr Leu Leu Arg 
                1045                1050                1055    
Asp Pro Ser Gly Phe Phe Trp Ser Ser Asp Ser Trp Ile Pro Gln Lys 
            1060                1065                1070        
Asp Phe Trp Ile Arg Val Lys Ser Arg Ile Gly Lys Ser Leu Arg Glu 
        1075                1080                1085            
Gln Ile Arg 
    1090    

<210> 3
<211> 106
<212> RNA
<213> other sequences


<220> 
<223> RNA tracr for B-Gen.1

<400> 3
uaauaaauaa uaugaguuac aggcucaugu cauuccgugc gugugcugcg aggcucuuac     60

aacgucucaa ccacgggauc acguuauuuc cacuaaguga uuggac                   106


<210> 4
<211> 109
<212> RNA
<213> other sequences


<220> 
<223> RNA tracr for B-Gen.2

<400> 4
cuggauauaa uaaagauguu uagcuauagg cuaauaagau aguuguguca agugcuucgg     60

agaccuaaca cgucauccag ucacaacggc uaucuauauu uccacuaac                109


<210> 5
<211> 106
<212> DNA
<213> other sequences


<220> 
<223> DNA encoding the RNA tracr for B-Gen.1

<400> 5
taataaataa tatgagttac aggctcatgt cattccgtgc gtgtgctgcg aggctcttac     60

aacgtctcaa ccacgggatc acgttatttc cactaagtga ttggac                   106


<210> 6
<211> 109
<212> DNA
<213> other sequences


<220> 
<223> DNA encoding the RNA tracr for B-Gen.2

<400> 6
ctggatataa taaagatgtt tagctatagg ctaataagat agttgtgtca agtgcttcgg     60

agacctaaca cgtcatccag tcacaacggc tatctatatt tccactaac                109


<210> 7
<211> 8715
<212> DNA
<213> other sequences


<220> 
<223> Plasmid pBLR373 including the insert for B-GEn.1

<400> 7
gctcagtcct aggtataatg ctagctaata cgactcacta taggagagac catggccacc       60

ggtctctgct aaagatatga ggagctcaaa ctggagctgc taacaaagcc cgaaaggaag      120

ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac      180

gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc      240

gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac      300

acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt      360

cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc      420

tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc      480

gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact      540

cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg      600

gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc      660

gaattttaac aaaatattaa cgtttacaat ttcaggtggc acttttcggg gaaatgtgcg      720

cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgaatta      780

attcttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat      840

caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt      900

tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac      960

aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga     1020

cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag     1080

gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg     1140

attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa     1200

tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag     1260

gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg     1320

catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc     1380

agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca     1440

gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc     1500

cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc     1560

gcggcctaga gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt     1620

ttatgtaagc agacagtttt attgttcatg accaaaatcc cttaacgtga gttttcgttc     1680

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg     1740

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg     1800

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca     1860

aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg     1920

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg     1980

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga     2040

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac     2100

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat     2160

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc     2220

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga     2280

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc     2340

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg     2400

gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag     2460

cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg     2520

catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg     2580

ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc     2640

ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc     2700

ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc     2760

accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca     2820

gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg     2880

gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc     2940

cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct     3000

cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca     3060

actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt     3120

cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg     3180

gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc     3240

gaataccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt     3300

tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg     3360

ggtcctcaac gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat     3420

ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc     3480

gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg     3540

gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa     3600

gaatacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac     3660

tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact     3720

tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct     3780

gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg     3840

tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag     3900

agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg     3960

tggttaacgg cgggatataa catgagctgt attcggtatc gtcgtatccc actaccgaga     4020

tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct     4080

gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt     4140

gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat     4200

tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg     4260

ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc     4320

gcgtaccgta ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa     4380

gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca     4440

gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt     4500

tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat     4560

cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg     4620

tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa     4680

tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc     4740

tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat     4800

cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc     4860

atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc     4920

ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc     4980

gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct     5040

gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc     5100

ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg     5160

gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca     5220

ctatagggga attgtgagcg gataacaatt cccctctaga tttacacttt atgcttccgg     5280

ctcgtatgtt tctagagaat tcaaataatt ttgtttaact ttaagaagga gatttaaata     5340

tgaaaatcga agaaggtaaa ggtcaccacc atcatcacca tatgaccatt cgctcgatga     5400

aattaaaact caagatttac tcaggccgct ctgcgccgca attacgccag ggtctgtggc     5460

gtttacaccg tctgctcaat gaaggcaccg cttattatat ggactggtta gtacacatgc     5520

gtcaagaagc cttaccgggc aaatcgaaag aggagattcg tgcggagctg gaacgccgtg     5580

ttcgccagca gcaagagaag aacggcgttc aaaacgatca agttcccatg gatgaggttc     5640

tgagtgcgct gcgtcagctg tacgaattac tggtgcccag tgcggttaac aacagcggcg     5700

acgcacagac cctcagccgc aagtttctga gcccgctggt cgacccgaac tcggaaggtg     5760

gcaaaggaac cagcaacgcc ggtgcaaaac cgggctggcg caagaagcaa gaagcgggcg     5820

atcctagctg ggaaaaggat tacgaacgtt ggttaaaacg caagcaggcg gatccgacgg     5880

ctgaaatttt aggcaaactg gaaacggcgg gtctgaagcc actgttccct ctgtacacaa     5940

acgaagtgaa agatatccgt tggatgccgt taacctcgaa acagtatgtg cgtaactggg     6000

atcgcgacat gtttcagcag gcgattgaac atctgttaag ctgggaaacc tggaaccgca     6060

aggtgaatga agaacgcgcc aaacttaaag aaacagtacg tcgcttcgaa gaacagcatt     6120

tagcgaatgg taaggattgg ctgagcccac tgcaggcata tgaagcaaac cgcgaacagg     6180

cgctgcgtga tatggcaatt tcgcctagcg accgttttcg catcacccgc cgtcagatta     6240

aaggctggtc ggaactttat gaacgttgga acaagctggc gcccacagcg tcagttgagg     6300

catacatgca agaggtacgt cacgtgcaga agaagctggg cgggacgttt ggtgatgcgg     6360

acttatatcg tttcctggca aagccggaga atgtgcacat ctggcgcgat catcaggaac     6420

gtctgcatta ttacgcagcg tacaacgacc ttcacaaacg ccttatgtcg gccaaagagc     6480

aagcggcctt taccctgccg gatccggtgg cgcatccgct gtgggtccgc ttcgatgcac     6540

gtgatggcaa ccttttcacc tacattctgc aagcggattc atcaaaacag cgctcccgcc     6600

gctatgttaa tttctcccgc tttctttggc cggtcgaaga tggttacttt gaagaaaccg     6660

aaaacgtgaa agtagaattg gcattgtcga agcagtttta tcgccaggtt atcgttcatg     6720

ataaccctac aggcaaacag aaaattacct ttcaagatta ttcgagcaag gaaattctgg     6780

aaggtcattt gggcggagcg aaactgcaac tggatcgcaa tttcctgcgt aagtccggac     6840

gtgattttga aacaggtgat ttcggaccgg cattcttgaa cgttgtatta gacctcaaac     6900

cgaaacaaga ggttaagaac ggacgcctgc aatctcctct gggccaggcg ttactggtga     6960

aaagtcgccc aaacgatatc ccgaaagttt acggctataa gcctgatgcc ctggcggcct     7020

ggttggaaca ggcctcgggt gaggaaagtt tgggcagtga aagcctgcgc cagggcttcc     7080

gtgtgatgag tatcgattta ggcgttcgca gtgccgccgc gattagcgtt ttctctgtga     7140

aaggcgagaa aacccgcgaa ggcgataaag tttgttaccc ggtgggcgaa acgggtctgt     7200

ttgcggtgca cgaccgtagc ttcttattgc gccttccggg agaatcatca gagaaacgtg     7260

tgaacgttga gcgtgataaa cgtaaaaccg aacgcatgca gattcgctat catattcgta     7320

ccctggcacg cgtactgcgt ctggccaata aagcgactcc tatggatcgc atcaaagcgg     7380

tgcaggatgt gctgaacgat attgaaagta cacgttttat gaatgaccac gaccaccatg     7440

tgtacaacca tgcactggaa acgctgcgca cttatgcgcc ggatcatcag ggcatctggg     7500

aagaacaggt cattgcggcc caccgtcaac tggagcatca tgtgggcgtg attgtgggcg     7560

aatggcgtaa gaactggggc aaagatcgtc gtggaaccgt cggcctgtcc atggacaaca     7620

ttgaggaatt agacgaaatg cgccgtctgc tcatctcatg gtcacgtcgc gcgcgttacc     7680

cgcgcgaagc taaaccattt caagtcaacg aaagtaaccc ggtccacctc ttacgtcatt     7740

tgcagaatct caaagaggat cgcttgaaac aattagctaa cttaattgtg atgaccgcgc     7800

ttggctacgt gtatgacagc aaagagaaga aatggaaagc ggcgtatccg gcatgccagc     7860

tgattctgtt tgaggatctg caacgctatc gctttcatct ggatcgtagc gcccgtgaga     7920

acagccaact gatgaaatgg gcgcatcgca gcattccaaa atatgtgtgg atgcaaggcg     7980

aaccgtatgg tctgcagatt ggagatgttt gggccggttt tacctcgcgc taccacgcga     8040

aaaccggtgc ccccggcatt cgttgcaaag cgttgaccga aaaggacttt caacagggtc     8100

gtctgctgga gtcgttggtc gccgaaggta tgttcacgct gcaggaagta ggtactctga     8160

aacctgggga tattgtgccg gcggaaggtg gagaactgtt tgtgaccttg gcggatgaca     8220

gtggggatcg tattgtgatc acccatgcgg atattaacgc agcgcagaat gtacagaaac     8280

gcttttggct cgcgaactcg gagcgctttc gcgtggcctg ccgctcggtg cagattgcca     8340

gccaggagtg cttcattcct agcagtgagt cggttgccaa gaaaatgggg aaaggcgtat     8400

ttgtgcgcga cttctcgttt cataaggata tggaagtgta tcattggaac aatcaagtca     8460

agctgaccgc aaagaatgta ccgaccgatc attctgacga tctgcaggat ctccaggatt     8520

atcaggcgat tcttgaagag gctcgcgaaa gttcatcaag ctacaaaacg ctgtttcgcg     8580

atcctagcgg gttcttcttt ccggatgatg tctgggtgcc ccaaaatatc tattggcgtg     8640

aagtcaagaa aaccattacc gcgttactgc gtaaacgtat tatgagcact taactcgagt     8700

aatgattgac agcta                                                      8715


<210> 8
<211> 8679
<212> DNA
<213> other sequences


<220> 
<223> Plasmid pBLR373 including the insert for B-GEn.2

<400> 8
gctcagtcct aggtataatg ctagctaata cgactcacta taggagagac catggccacc       60

ggtctctgct aaagatatga ggagctcaaa ctggagctgc taacaaagcc cgaaaggaag      120

ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac      180

gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc      240

gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac      300

acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt      360

cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc      420

tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc      480

gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact      540

cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg      600

gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc      660

gaattttaac aaaatattaa cgtttacaat ttcaggtggc acttttcggg gaaatgtgcg      720

cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgaatta      780

attcttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat      840

caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt      900

tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac      960

aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga     1020

cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag     1080

gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg     1140

attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa     1200

tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag     1260

gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg     1320

catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc     1380

agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca     1440

gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc     1500

cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc     1560

gcggcctaga gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt     1620

ttatgtaagc agacagtttt attgttcatg accaaaatcc cttaacgtga gttttcgttc     1680

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg     1740

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg     1800

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca     1860

aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg     1920

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg     1980

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga     2040

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac     2100

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat     2160

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc     2220

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga     2280

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc     2340

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg     2400

gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag     2460

cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg     2520

catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg     2580

ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc     2640

ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc     2700

ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc     2760

accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca     2820

gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg     2880

gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc     2940

cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct     3000

cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca     3060

actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt     3120

cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg     3180

gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc     3240

gaataccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt     3300

tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg     3360

ggtcctcaac gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat     3420

ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc     3480

gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg     3540

gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa     3600

gaatacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac     3660

tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact     3720

tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct     3780

gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg     3840

tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag     3900

agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg     3960

tggttaacgg cgggatataa catgagctgt attcggtatc gtcgtatccc actaccgaga     4020

tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct     4080

gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt     4140

gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat     4200

tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg     4260

ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc     4320

gcgtaccgta ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa     4380

gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca     4440

gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt     4500

tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat     4560

cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg     4620

tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa     4680

tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc     4740

tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat     4800

cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc     4860

atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc     4920

ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc     4980

gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct     5040

gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc     5100

ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg     5160

gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca     5220

ctatagggga attgtgagcg gataacaatt cccctctaga tttacacttt atgcttccgg     5280

ctcgtatgtt tctagagaat tcaaataatt ttgtttaact ttaagaagga gatttaaata     5340

tgaaaatcga agaaggtaaa ggtcaccacc atcatcacca tatgcctatt cgctccttta     5400

aactgaaact ggtgacacat aacggcgata gcacatatat ggataaactt cgtcgcggtc     5460

tgtggaaaac gcacgttatc atcaatcgcg gtatcgccta ttatatgaac accctcgcgc     5520

tgatgcgcca ggaaccgtac ggctcgaaat ctcgtgaaga agtgcgcctg gatttgctct     5580

cgacgttacg tgaacagcaa cgtcgcaata actggtccga gcaaacaggt actgacgatg     5640

agctcctttc gctgagtcgc cgcgtctacg aactcttggt gccctcggca atcggcgaaa     5700

agggcgacgc gcagatgctg agtcgtaaat tcctgagccc gttggttgac ccgaatagcg     5760

aaggcggccg cggaaccgcg aaaagcggtc gcaaaccgcg ttggaagaag atgatggaag     5820

aagggcatcc ggattgggaa aaggagaaag agaaagatgc cgcgaagaag gccgaagatc     5880

cgacagccag tattctcgcg gatctcgaag ccgtaggcct tctgccgctc tttcctttgt     5940

tcagcgacga acagaaagag atccgctggc tccccaagaa gaaacgccag tttgtacgta     6000

cgtgggatcg cgatatgttt cagcaggccc tggaacgtat gctgtcttgg gagagttgga     6060

accgccgtgt tgcggaagag tacctgaaac tgcaggcgca gcgcgatgag gtgtacgcga     6120

agtatctcga agatgccggc tcctggttga acgatttaca gacctttgag aaacagcgtg     6180

aagaggagct cgcagaggtg agttttgaac cgaactccga gtacctcatc actcgtcgcc     6240

agatccgcgg ttggaaagaa gtctatgaga aatggtcgaa aaccagcgaa aatgcctcac     6300

aggaacagct gtggcgtatg gtggcggacg tgcagaccgc gatggcagga gcatttggag     6360

atccaaaagt gtaccagttc ttgagccagc ctaaacacca ccacatctgg cgcgaacacc     6420

cgaaccgtct gttttactat tcgaaataca atgaagtccg tgagaaattg aaccgcgcaa     6480

agaagcaagc cgcatttacg ctcccggacc cggttgaaca tccactttgg acccgctttg     6540

atgcgcgcgg cggtaacatc catgactatg aaatcagcaa agtagggaaa cagtatcatg     6600

ttaccttttc gtcactgatt cttccggaag cacagagctg ggtggaaatc gaaaatgtga     6660

cggtcggcat tggcaattca ctgcaattaa aacgccaaat tcgtttagat ggttacgctg     6720

acaagaaaca gaaagtgaag tattacgact atagcagccg cttcgaactt accggcgtgc     6780

tgggtggtgc gaagattcag tttgatcgta aacatctgaa gaaggcggcg caccgcttag     6840

ctgaaggtga gactggcccg attttcctca acgtggtagt ggacgtggag ccctttcttg     6900

aagtaaagaa cggacgcttg cgtacccctc tgggtcaggt gttacaagtg aatacccgtg     6960

attggccgaa ggttgtcgac tacaaagcga aagaactctc ggtgctgatg gaaaataccc     7020

agatcggtaa cgagaatggc gtatccacaa ttgaagctgg aatgcgtatt atgagcattg     7080

atctgggcca gcgcaccgcg gctgccgtgt ccattttcga agtaatcagc aagaagccgg     7140

acgaaaagga aaccaaatta ttctatccga tcgctgatac cgacctgtac gccgtgcacc     7200

gccgcagttt actgctgcgt ctgccgggag aggaaattag cagtaagaag atgattgaaa     7260

agcgcaaaga acgtgcgcgc atccgtagct tagtgcgcta tcagattcgc ctgctgagtg     7320

aggttctgcg tctgcacacc cagggaacgg cggagcaacg tcgttttaag ctggatgagt     7380

tgctggtcag cattcagaag aagttagaat tggatcaaag tgagtggatt agtgaattag     7440

aaaagttatt cgattatatc gacgaaagcg cggagaagtg gaaggaagca ctggtagtgg     7500

cgcaccgcac cttggaaccg attgtggtag aagccgtgcg taattggaag aagtcacttt     7560

ctaaggaaaa taaagaccgc cgccgtatcg ccggcatttc aatctggagt attgaagaac     7620

ttgaagaaac gcgtaagtta ctgattgctt ggtccaaaca ttcccgtgaa ccaggaatcc     7680

ctaagcgcct ggagaaagag gaaaccttcg cgcctgaaca tctgcaacac atccaaaatg     7740

ttaaggatga tcgtctgaaa cagatggcaa acctgtttgt tatgacagcg ttgggctata     7800

aatatgatga aggcaataag cgctgggttg aggcataccc ggcatgccag gtgattctgt     7860

ttgaggatct cagccgttac cgttttgccc tggaccgtcc gcgccgcgag aataaccgct     7920

taatgaaatg ggcccaccgc agtattccgc gtctgaccta catgcaggcc gagctgtttg     7980

gcattcaagt aggcgatgtt tactcagcct acactagccg cttccatgca aagaccgggg     8040

cgccgggcat ccgctgccac gccctgacag aggcggacct gcaatccaat agctatgtgg     8100

taaaccagtt gattaaggat aaattcatcc aagacaatca gaccgaaatc ctgaaagcgg     8160

gccagatcgt cccgtggcag ggcggcgaac tcttcgtgac gttcgcggat cgcagtggcg     8220

ccagcttggc agtcattcat gccgacatta atgccgcgca gaatcttcag aaacgctttt     8280

ggcaacacaa ctcggaagtg tttcgcgtcc cgtgcaaagt ggtgaaaggt ggcctcgttc     8340

cggtgtacga gaaaatgcgc aaactgttcg ggaaaggatt atttgtcaac atcgacgatc     8400

ccgaaagcaa ggaagtgtac cgttgggaac atagcacgaa aatgaaatca aagacgaccc     8460

ctgtcgattt ggagtcagaa gatatcgatc acgaggaatt aagcgatgag tgggaagata     8520

tgcaggaagg ctacaagacg ctgcttcgcg acccttcggg tttcttttgg tcgagtgact     8580

cttggattcc tcagaaagat ttctggattc gcgtgaaaag ccgcatcggc aaatcgcttc     8640

gcgagcagat tcgttaactc gagtaatgat tgacagcta                            8679


<210> 9
<211> 8018
<212> DNA
<213> other sequences


<220> 
<223> expression construct containing B-Gen.1 with N-terminal linker, a
      C-terminal linker, T2A-GFP

<400> 9
aaagtagagg aacttcactt cattttgcta gaccggtacc gcgttacata acttacggta       60

aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat agtaacgcca      120

atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca      180

gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg      240

cccgcctggc attgtgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc      300

tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc      360

cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt      420

gcagcgatgg gggcgggggg gggggggcgc gcgccaggtg gggcggggcg gggcgagggg      480

cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag      540

tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg      600

gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc      660

ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct      720

cctccgggct gtaattagct gagcaagagg taagggttta agggatggtt ggttggtggg      780

gtattaatgt ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggcaac      840

cgcggacacc caagcggccg ccaatgaaga ggcctgccgc cacaaagaag gccggccagg      900

ccaagaagaa gaagggtggc ggtggtagtg gtggcggtgg ttcaggcggt ggcggctcag      960

gctccatgcc tattcgctct tttaagctga aacttgtaac ccataacggc gatagtacgt     1020

acatggataa actgcgccgc ggcctgtgga aaactcatgt gataataaac cgcggcatcg     1080

cgtactacat gaacaccttg gccctcatga ggcaagaacc ctacgggtcc aagagccggg     1140

aagaagtacg gttggatctg ctttccactc tgagagagca gcaacgccgg aacaattggt     1200

cagaacaaac cgggactgat gatgaattgc tttccctgag ccgtcgagtt tacgagcttc     1260

ttgtaccatc cgctataggt gaaaagggcg atgcgcaaat gctgagccgc aaatttctct     1320

cccctctcgt ggacccaaac agtgagggcg ggcgcgggac cgccaaaagt gggagaaagc     1380

cacggtggaa gaagatgatg gaagaaggac accccgactg ggaaaaggag aaggaaaagg     1440

atgcggctaa gaaggcagag gatccaacag catctatatt ggcggatctc gaggcagtgg     1500

gactgttgcc acttttcccg ctgttttctg acgaacagaa agaaatccgc tggctcccca     1560

agaagaagcg tcagtttgtg aggacatggg accgggatat gttccaacag gcccttgaac     1620

gaatgctgag ctgggaaagc tggaatcgca gagttgccga ggaatacttg aagctccagg     1680

cccagcggga cgaggtgtat gccaaatacc ttgaggatgc tggctcctgg cttaatgatc     1740

tgcagacttt cgaaaagcag cgcgaagagg aactggcgga agtaagcttc gaacccaact     1800

ctgagtacct gattactcgc cggcagattc gcgggtggaa ggaagtgtat gaaaagtggt     1860

caaagaccag cgaaaatgcg agccaggagc agttgtggcg gatggtggct gacgtgcaga     1920

ctgccatggc cggcgcgttc ggagatccca aagtgtacca gtttctttct cagcctaagc     1980

accaccatat ttggagagag catccaaata gactgttcta ctacagcaag tacaacgagg     2040

tccgggagaa gctcaacagg gccaagaagc aggcggcctt taccctgcca gatcccgtgg     2100

agcacccact gtggacacgt tttgatgcgc gcggcggcaa tatccatgac tacgagatat     2160

ccaaggtggg caagcaatac cacgtgacgt tcagctctct tatcctgcca gaggcccaga     2220

gttgggtgga gattgagaat gttacagtgg gtatcgggaa cagcctgcag ctgaaacgcc     2280

agattcgctt ggacggttac gcagacaaga agcagaaagt caaatattat gattattcca     2340

gcaggttcga gctcacagga gtactcggcg gagcaaagat ccaattcgat aggaagcatc     2400

ttaagaaggc tgctcacaga ctggctgagg gcgaaacagg gccgatcttc ttgaatgttg     2460

tggtggatgt agaaccattc ttggaagtga agaatggacg tttgcggact ccactcggtc     2520

aagtcctgca ggtgaacact agagattggc ccaaggtcgt cgactacaaa gcaaaggagc     2580

tgtcagtctt gatggaaaac acgcaaattg gcaacgagaa tggggtgtct acaatcgagg     2640

ccggaatgag gattatgtct attgacctgg gacagaggac cgctgctgcc gtgtctattt     2700

tcgaggtcat ttctaagaaa cctgacgaaa aggaaacgaa gctgttttat cccatcgcag     2760

acacagatct gtatgccgtt cacaggcgaa gtctcctgct gagattgccc ggcgaggaga     2820

tctctagtaa gaaaatgatt gagaagcgga aagagcgcgc acgtatccgc tccctggtga     2880

ggtatcagat caggctcctc tcagaagtcc tgcggcttca cacacagggc accgctgaac     2940

agcgtcggtt taagctggac gagctgctcg tgagcatcca gaagaagttg gagcttgacc     3000

agagcgagtg gatttcagaa ctcgagaaac tcttcgacta tatcgatgag tcagctgaga     3060

aatggaaaga ggctctggtc gtggctcaca gaactctgga gcctatcgtg gttgaagccg     3120

tcagaaactg gaagaagtct ctgtccaaag aaaacaagga tagacgcagg attgccggaa     3180

tctccatctg gtctatagag gagctcgagg aaacccgcaa gctgcttatt gcatggtcta     3240

aacactccag agaacctggc attccgaaaa gacttgagaa agaggaaaca tttgctccag     3300

aacacctgca gcacatacag aacgtgaaag acgatcgcct taaacagatg gctaacttgt     3360

ttgtgatgac cgccttgggc tataagtatg atgaaggcaa taagcggtgg gtggaagcgt     3420

atccagcatg ccaggtaatc ctgtttgagg atttgtctag atatcggttc gccctggaca     3480

gacctaggcg ggagaacaat cgattgatga agtgggccca ccgcagtatc ccaaggctca     3540

cctacatgca ggctgaactg tttggaatcc aggtgggcga tgtctattca gcgtacacct     3600

ctagattcca cgccaaaaca ggggcccccg ggatccgctg tcatgcactg acagaagccg     3660

atcttcaatc taatagttac gttgtgaacc agctgataaa ggataaattt attcaagaca     3720

accagactga aatcctcaag gccggccaga tagttccttg gcagggcggt gagttgtttg     3780

ttacctttgc agatagatcc ggcgccagct tggctgttat ccacgccgat attaatgcag     3840

cgcagaacct tcagaaaagg ttttggcagc acaactcaga ggtgttccga gtgccttgta     3900

aggtggtgaa gggcgggttg gtccctgttt atgaaaagat gcgaaagctt ttcggaaaag     3960

gcctctttgt aaatatagac gacccagaat ccaaggaagt ctaccgctgg gaacattcca     4020

ctaagatgaa atccaagact acaccagttg accttgaaag cgaagatatt gatcacgagg     4080

aactcagcga cgagtgggaa gatatgcagg aaggatacaa aacactgctg cgtgaccctt     4140

caggcttctt ctggtcctca gacagttgga tccctcagaa ggatttctgg atcagagtca     4200

agagcagaat cggcaagtct ctgcgggagc agattcgcgg tggaagcgga ggcagcgggg     4260

gatcaggtcc ccctaagaaa aagcggaagg tgggcggtgg cggcggttca catcatcatc     4320

accatcatgg cggtgaagga cgcggctccc ttctgacctg tggtgacgtg gaagagaacc     4380

ccggccctga attcatgtct ggcggcgagg aactgtttgc cggaatcgtg cctgtgctga     4440

tcgagctgga tggggatgtg cacggccaca agtttagcgt cagaggcgaa ggcgaagggg     4500

acgccgatta cggcaagctg gaaatcaagt tcatctgcac caccggaaag ctgcccgtgc     4560

cttggcctac actggtcacc acactgtgct acggcatcca gtgcttcgcc agatatcccg     4620

agcatatgaa gatgaacgac ttcttcaaga gcgccatgcc tgagggctac atccaagagc     4680

ggacaatcca gttccaggac gacggcaagt acaagaccag gggcgaagtg aagttcgagg     4740

gcgacaccct ggtcaacaga atcgagctga agggcaaaga cttcaaagag gacggcaaca     4800

tcctgggcca caagcttgag tacagcttca acagccacaa cgtgtacatc agacccgaca     4860

aggccaacaa cggcctggaa gccaacttca agacccggca caacatcgaa ggtggcggag     4920

tgcagctggc cgatcactac cagacaaatg tgcccctcgg agatggcccc gtgctgatcc     4980

ctatcaacca ctacctgagc acccagacca agatcagcaa ggacagaaac gaggcccggg     5040

accacatggt gctgctggaa agctttagcg cctgctgtca cacccacggc atggacgagc     5100

tgtacagata atgactcgag atcagcctcg actgtgcctt ctagttgcca gccatctgtt     5160

gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc     5220

taataaaatg aggaaattgc atccccactt cagaagttcc tatactttct aatgagtgag     5280

ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg     5340

ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc     5400

ttgcgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc     5460

agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa     5520

catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt     5580

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg     5640

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg     5700

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag     5760

cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc     5820

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa     5880

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg     5940

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc     6000

taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac     6060

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg     6120

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt     6180

gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt     6240

catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa     6300

atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga     6360

ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt     6420

gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg     6480

tgacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga     6540

gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga     6600

agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg     6660

catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc     6720

aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc     6780

gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca     6840

taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac     6900

caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg     6960

ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc     7020

ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg     7080

tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac     7140

aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat     7200

actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata     7260

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcatat ttccccgaaa     7320

agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt     7380

acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag     7440

gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgaacaa     7500

actctttctg tgttgtaaaa cgacggccag tcttaagctc gggccccctg ggcggttctg     7560

ataacgagta atcgttaatc cgcaaataac gtaaaaaccc gcttcggcgg gtttttttat     7620

ggggggagtt tagggaaaga gcatttgtca gaatatttaa gggcgcctgt cactttgctt     7680

gatatatgag aattatttaa ccttataaat gagaaaaaag caacgcactt taaataagat     7740

acgttgcttt ttcgattgat gaacacctat aattaaacta ttcatctatt atttatgatt     7800

ttttgtatat acaatatttc tagtttgtta aagagaatta agaaaataaa tctcgaaaat     7860

aataaaggga aaatcagttt ttgatatcaa aattatacat gtcaacgata atacaaaata     7920

taatacaaac tataagatgt tatcagtatt tattatgcat ttagaataaa ttttgtgtcg     7980

cccttattcg actcactata gaagttccta ttctctag                             8018


<210> 10
<211> 8054
<212> DNA
<213> other sequences


<220> 
<223> expression construct containing B-Gen.2 with N-terminal linker, a
      C-terminal linker, T2A-GFP

<400> 10
ctctagaaag tagaggaact tcacttcatt ttgctagacc ggtaccgcgt tacataactt       60

acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaatagta      120

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac      180

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt      240

aaatggcccg cctggcattg tgcccagtac atgaccttat gggactttcc tacttggcag      300

tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc      360

actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta      420

ttttgtgcag cgatgggggc gggggggggg gggcgcgcgc caggtggggc ggggcggggc      480

gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc      540

cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg      600

cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg      660

cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc      720

ccttctcctc cgggctgtaa ttagctgagc aagaggtaag ggtttaaggg atggttggtt      780

ggtggggtat taatgtttaa ttacctggag cacctgcctg aaatcacttt ttttcaggtt      840

ggcaaccgcg gacacccaag cggccgccaa tgaagaggcc tgccgccaca aagaaggccg      900

gccaggccaa gaagaagaag ggtggcggtg gtagtggtgg cggtggttca ggcggtggcg      960

gctcaggctc catgaccatc agaagtatga agctgaagct taaaatctat agcggcaggt     1020

ccgcgcccca actcaggcag ggtctgtggc gtttgcatcg gctgttgaac gagggaacgg     1080

cctattacat ggattggctt gtacatatga gacaagaggc tctcccaggc aaaagcaaag     1140

aagagattag agctgaattg gagagaagag tgagacagca gcaggaaaag aatggtgtgc     1200

aaaatgacca ggtcccgatg gacgaagtcc tcagcgcact taggcaactg tatgagcttc     1260

tggtcccgtc tgccgtaaat aattccggcg acgcacagac gctgtctagg aagttcttgt     1320

ctccattggt ggacccaaac tccgagggtg gaaagggcac gtcaaacgcc ggagccaagc     1380

ctggctggcg gaagaaacaa gaggcagggg acccaagctg ggagaaagac tatgagagat     1440

ggctcaaacg caagcaggct gaccctaccg ctgagatttt gggtaagctg gaaaccgccg     1500

gcctcaaacc cctcttcccc ctgtacacaa atgaagtcaa ggatataagg tggatgccac     1560

tgacgtctaa acaatatgtc cgaaactggg atcgcgatat gttccaacaa gctatcgagc     1620

atctgctcag ttgggaaact tggaatagaa aggtgaatga ggaaagggcg aaactgaagg     1680

aaacagtcag gcgctttgaa gaacagcacc tggccaatgg aaaggattgg ctctccccac     1740

ttcaggccta tgaagccaat cgagaacagg cacttagaga catggccata tctcctagcg     1800

atcgcttcag gatcactagg cgccaaataa agggatggtc tgagctttat gaaaggtgga     1860

acaagctcgc acctaccgct agcgtagagg cgtacatgca ggaagttcgc catgtacaga     1920

agaaactcgg cggtaccttt ggagatgccg acctctaccg gtttctggcc aaacccgaga     1980

acgtgcacat atggcgcgac caccaggagc ggctgcacta ctatgccgca tacaatgatt     2040

tgcataagcg actcatgagc gccaaagagc aggctgcttt tacactcccc gaccctgtgg     2100

cccatccact gtgggtgcgc tttgacgcca gagatggcaa tctgtttact tacatactgc     2160

aggccgactc ttcaaagcag aggtcacgta gatacgtcaa tttctctcgt tttctgtggc     2220

ctgttgaaga tgggtacttt gaggaaacgg agaacgtcaa agttgagctg gctctgtcca     2280

agcaatttta tagacaggtg attgtccacg acaacccaac gggaaagcag aagataacgt     2340

tccaggatta ctccagtaaa gaaatcctcg aagggcatct tggcggggcc aaactgcagc     2400

tcgaccggaa tttccttcgg aaatcagggc gggactttga gacaggcgat tttgggccgg     2460

cttttctgaa cgtggtgttg gacctgaagc caaaacagga agtgaagaat gggcgcttgc     2520

agtcccctct tggccaggcg ttgctcgtta aaagcagacc caacgatatc ccgaaggtgt     2580

acggatacaa accagatgca ttggcagctt ggctggagca agccagtggt gaagagtccc     2640

tggggagcga gtcattgcgt caggggttta gagtaatgtc aattgatctc ggcgtcaggt     2700

ctgccgctgc tatatccgtc tttagtgtga aaggagagaa aacccgtgag ggcgataagg     2760

tgtgctatcc agttggggag acaggcctct tcgccgtcca cgataggtct tttctgctca     2820

gactcccggg tgaatcaagc gagaagcggg tgaatgtgga gcgtgataaa cgtaagacag     2880

agagaatgca gatccgttat cacatcagga ccctggcgcg cgtgttgaga ctcgcaaaca     2940

aggcaacccc aatggaccgg atcaaggccg tgcaggatgt gctgaacgat atcgagtcta     3000

ctaggttcat gaatgaccat gaccaccacg tttacaacca tgctctcgaa accctcagaa     3060

cctacgctcc tgatcaccag gggatctggg aagaacaagt aatcgccgcc catcgccagc     3120

tcgaacacca cgtcggcgtt atcgtagggg agtggcggaa gaactggggt aaagatcgtc     3180

gagggacagt cgggctgagc atggacaaca ttgaagaact cgatgagatg aggagattgc     3240

tgatctcttg gagccgccgg gctcgttacc ctcgcgaggc gaagcctttc caagtgaacg     3300

agtcaaaccc tgtgcacctg ttgcggcatc tgcagaattt gaaagaggac agactgaagc     3360

agcttgccaa cctgattgtg atgaccgccc tgggctatgt gtacgactcc aaggaaaaga     3420

agtggaaagc tgcataccct gcatgccagc tgatcctttt cgaggacctg cagaggtaca     3480

ggttccacct cgaccgtagt gccagggaaa actcacagct gatgaaatgg gcacataggt     3540

caatcccaaa gtatgtgtgg atgcagggtg agccctacgg acttcaaatc ggcgatgtgt     3600

gggcaggctt cacttcccgg taccacgcga aaaccggggc tcccggaatt cggtgtaaag     3660

cactgaccga aaaggatttt cagcagggcc gacttctgga gtcactcgtc gcggaaggca     3720

tgtttaccct gcaggaagtg ggcacgttga aaccagggga catcgttccc gcggaaggcg     3780

gcgaactttt cgtgactctg gccgatgact ctggagacag gatcgtcatc acccacgcag     3840

atattaacgc agctcagaat gtccagaagc gattttggct cgcaaatagc gagaggttcc     3900

gcgttgcatg tcgcagcgtg caaatcgcat cccaggagtg ttttattcct tccagcgagt     3960

ctgtcgccaa gaagatgggc aagggagtct ttgtgcggga cttctccttt cacaaagaca     4020

tggaagtgta ccactggaat aaccaggtca agctcacagc aaagaacgta ccgaccgacc     4080

acagcgatga tctccaggac ctgcaggact accaggctat cctcgaagaa gccagggagt     4140

ctagtagcag ctacaagacg ctgtttaggg atccttctgg gttcttcttc ccagacgatg     4200

tgtgggtccc tcagaatatt tactggcgag aggttaagaa aacaatcacc gctctgctgc     4260

gtaagcgcat catgtccacc ggtggaagcg gaggcagcgg gggatcaggt ccccctaaga     4320

aaaagcggaa ggtgggcggt ggcggcggtt cacatcatca tcaccatcat ggcggtgaag     4380

gacgcggctc ccttctgacc tgtggtgacg tggaagagaa ccccggccct gaattcatgt     4440

ctggcggcga ggaactgttt gccggaatcg tgcctgtgct gatcgagctg gatggggatg     4500

tgcacggcca caagtttagc gtcagaggcg aaggcgaagg ggacgccgat tacggcaagc     4560

tggaaatcaa gttcatctgc accaccggaa agctgcccgt gccttggcct acactggtca     4620

ccacactgtg ctacggcatc cagtgcttcg ccagatatcc cgagcatatg aagatgaacg     4680

acttcttcaa gagcgccatg cctgagggct acatccaaga gcggacaatc cagttccagg     4740

acgacggcaa gtacaagacc aggggcgaag tgaagttcga gggcgacacc ctggtcaaca     4800

gaatcgagct gaagggcaaa gacttcaaag aggacggcaa catcctgggc cacaagcttg     4860

agtacagctt caacagccac aacgtgtaca tcagacccga caaggccaac aacggcctgg     4920

aagccaactt caagacccgg cacaacatcg aaggtggcgg agtgcagctg gccgatcact     4980

accagacaaa tgtgcccctc ggagatggcc ccgtgctgat ccctatcaac cactacctga     5040

gcacccagac caagatcagc aaggacagaa acgaggcccg ggaccacatg gtgctgctgg     5100

aaagctttag cgcctgctgt cacacccacg gcatggacga gctgtacaga taatgactcg     5160

agatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg     5220

ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt     5280

gcatccccac ttcagaagtt cctatacttt ctaatgagtg agctaactca cattaattgc     5340

gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat     5400

cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttgcgctt cctcgctcac     5460

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     5520

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     5580

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     5640

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     5700

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     5760

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     5820

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     5880

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     5940

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     6000

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     6060

aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     6120

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     6180

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     6240

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     6300

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     6360

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     6420

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg     6480

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgtgacccac gctcaccggc     6540

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     6600

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     6660

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     6720

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     6780

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     6840

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     6900

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     6960

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     7020

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     7080

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     7140

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     7200

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     7260

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     7320

gaaaaataaa caaatagggg ttccgcgcat atttccccga aaagtgccac ctgacgtcga     7380

cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc     7440

gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg     7500

agcaaaattt aagctacaac aaggcaaggc ttgaccgaac aaactctttc tgtgttgtaa     7560

aacgacggcc agtcttaagc tcgggccccc tgggcggttc tgataacgag taatcgttaa     7620

tccgcaaata acgtaaaaac ccgcttcggc gggttttttt atggggggag tttagggaaa     7680

gagcatttgt cagaatattt aagggcgcct gtcactttgc ttgatatatg agaattattt     7740

aaccttataa atgagaaaaa agcaacgcac tttaaataag atacgttgct ttttcgattg     7800

atgaacacct ataattaaac tattcatcta ttatttatga ttttttgtat atacaatatt     7860

tctagtttgt taaagagaat taagaaaata aatctcgaaa ataataaagg gaaaatcagt     7920

ttttgatatc aaaattatac atgtcaacga taatacaaaa tataatacaa actataagat     7980

gttatcagta tttattatgc atttagaata aattttgtgt cgcccttatt cgactcacta     8040

tagaagttcc tatt                                                       8054


<210> 11
<211> 164
<212> DNA
<213> other sequences


<220> 
<223> ATTA PAM containing guide sequence

<400> 11
gctggatata ataaagatgt ttagctatag gctaataaga tagttgtgtc aagtgcttcg     60

gagacctaac acgtcatcca gtcacaacgg ctatctatat ttccactaac aaaagttagt    120

ggaaatgtag atggttagca ctggacaagt agcaagaaat taat                     164


<210> 12
<211> 164
<212> DNA
<213> other sequences


<220> 
<223> ATTT PAM containing guide sequence

<400> 12
gctggatata ataaagatgt ttagctatag gctaataaga tagttgtgtc aagtgcttcg     60

gagacctaac acgtcatcca gtcacaacgg ctatctatat ttccactaac aaaagttagt    120

ggaaatgtag atggttagca cgttttcttt agttattaat ttct                     164


<210> 13
<211> 164
<212> DNA
<213> other sequences


<220> 
<223> ATTC PAM containing guide sequence

<400> 13
gctggatata ataaagatgt ttagctatag gctaataaga tagttgtgtc aagtgcttcg     60

gagacctaac acgtcatcca gtcacaacgg ctatctatat ttccactaac aaaagttagt    120

ggaaatgtag atggttagca ccattaacta gcattataat gcac                     164


<210> 14
<211> 164
<212> DNA
<213> other sequences


<220> 
<223> ATTG PAM containing guide sequence

<400> 14
gctggatata ataaagatgt ttagctatag gctaataaga tagttgtgtc aagtgcttcg     60

gagacctaac acgtcatcca gtcacaacgg ctatctatat ttccactaac aaaagttagt    120

ggaaatgtag atggttagca ctttagtgac tgtaattttc tttt                     164


<210> 15
<211> 164
<212> DNA
<213> other sequences


<220> 
<223> ATTT PAM containing guide sequence

<400> 15
gctggatata ataaagatgt ttagctatag gctaataaga tagttgtgtc aagtgcttcg     60

gagacctaac acgtcatcca gtcacaacgg ctatctatat ttccactaac aaaagttagt    120

ggaaatgtag atggttagca cgtgaagtct tacaaggtta tctt                     164


<210> 16
<211> 164
<212> DNA
<213> other sequences


<220> 
<223> ATTG PAM containing guide sequence

<400> 16
gctggatata ataaagatgt ttagctatag gctaataaga tagttgtgtc aagtgcttcg     60

gagacctaac acgtcatcca gtcacaacgg ctatctatat ttccactaac aaaagttagt    120

ggaaatgtag atggttagca ctttagtgac tgtaattttc tttt                     164


<210> 17
<211> 164
<212> DNA
<213> other sequences


<220> 
<223> ATTC PAM containing guide sequence

<400> 17
gctggatata ataaagatgt ttagctatag gctaataaga tagttgtgtc aagtgcttcg     60

gagacctaac acgtcatcca gtcacaacgg ctatctatat ttccactaac aaaagttagt    120

ggaaatgtag atggttagca caatcttcaa ccctattctg tgaa                     164


<210> 18
<211> 164
<212> DNA
<213> other sequences


<220> 
<223> ATTA PAM containing guide sequence

<400> 18
gctggatata ataaagatgt ttagctatag gctaataaga tagttgtgtc aagtgcttcg     60

gagacctaac acgtcatcca gtcacaacgg ctatctatat ttccactaac aaaagttagt    120

ggaaatgtag atggttagca cataactaaa gaaaacaaat ctta                     164


<210> 19
<211> 164
<212> DNA
<213> other sequences


<220> 
<223> GTTA PAM containing guide sequence

<400> 19
gctggatata ataaagatgt ttagctatag gctaataaga tagttgtgtc aagtgcttcg     60

gagacctaac acgtcatcca gtcacaacgg ctatctatat ttccactaac aaaagttagt    120

ggaaatgtag atggttagca cgtacagttt tgctgaaagg ttat                     164


<210> 20
<211> 164
<212> DNA
<213> other sequences


<220> 
<223> GTTC PAM containing guide sequence

<400> 20
gctggatata ataaagatgt ttagctatag gctaataaga tagttgtgtc aagtgcttcg     60

gagacctaac acgtcatcca gtcacaacgg ctatctatat ttccactaac aaaagttagt    120

ggaaatgtag atggttagca ctgcttatta tggacaagta gcaa                     164


<210> 21
<211> 164
<212> DNA
<213> other sequences


<220> 
<223> GTTT PAM containing guide sequence

<400> 21
gctggatata ataaagatgt ttagctatag gctaataaga tagttgtgtc aagtgcttcg     60

gagacctaac acgtcatcca gtcacaacgg ctatctatat ttccactaac aaaagttagt    120

ggaaatgtag atggttagca ctattccatt aactagcatt ataa                     164


<210> 22
<211> 164
<212> DNA
<213> other sequences


<220> 
<223> GTTT PAM containing guide sequence

<400> 22
gctggatata ataaagatgt ttagctatag gctaataaga tagttgtgtc aagtgcttcg     60

gagacctaac acgtcatcca gtcacaacgg ctatctatat ttccactaac aaaagttagt    120

ggaaatgtag atggttagca ccagtgcgac gccgcgagcc ccga                     164


<210> 23
<211> 164
<212> DNA
<213> other sequences


<220> 
<223> GTTG PAM containing guide sequence

<400> 23
gctggatata ataaagatgt ttagctatag gctaataaga tagttgtgtc aagtgcttcg     60

gagacctaac acgtcatcca gtcacaacgg ctatctatat ttccactaac aaaagttagt    120

ggaaatgtag atggttagca cgacgaaaag tttcagtgcg acgc                     164


<210> 24
<211> 164
<212> DNA
<213> other sequences


<220> 
<223> GTTC PAM containing guide sequence

<400> 24
gctggatata ataaagatgt ttagctatag gctaataaga tagttgtgtc aagtgcttcg     60

gagacctaac acgtcatcca gtcacaacgg ctatctatat ttccactaac aaaagttagt    120

ggaaatgtag atggttagca ctcgcttcgg aggagccgtg gtcc                     164


<210> 25
<211> 170
<212> DNA
<213> other sequences


<220> 
<223> ATTA PAM containing guide sequence

<400> 25
gtaataaata atatgagtta caggctcatg tcattccgtg cgtgtgctgc gaggctctta     60

caacgtctca accacgggat cacgttattt ccactaagtg attggacaaa agtcagatta    120

tttagtggaa agtaacgtga ttagcactgg acaagtagca agaaattaat               170


<210> 26
<211> 170
<212> DNA
<213> other sequences


<220> 
<223> ATTT PAM containing guide sequence

<400> 26
gtaataaata atatgagtta caggctcatg tcattccgtg cgtgtgctgc gaggctctta     60

caacgtctca accacgggat cacgttattt ccactaagtg attggacaaa agtcagatta    120

tttagtggaa agtaacgtga ttagcacgtt ttctttagtt attaatttct               170


<210> 27
<211> 170
<212> DNA
<213> other sequences


<220> 
<223> ATTC PAM containing guide sequence

<400> 27
gtaataaata atatgagtta caggctcatg tcattccgtg cgtgtgctgc gaggctctta     60

caacgtctca accacgggat cacgttattt ccactaagtg attggacaaa agtcagatta    120

tttagtggaa agtaacgtga ttagcaccat taactagcat tataatgcac               170


<210> 28
<211> 170
<212> DNA
<213> other sequences


<220> 
<223> ATTG PAM containing guide sequence

<400> 28
gtaataaata atatgagtta caggctcatg tcattccgtg cgtgtgctgc gaggctctta     60

caacgtctca accacgggat cacgttattt ccactaagtg attggacaaa agtcagatta    120

tttagtggaa agtaacgtga ttagcacttt agtgactgta attttctttt               170


<210> 29
<211> 170
<212> DNA
<213> other sequences


<220> 
<223> ATTT PAM containing guide sequence

<400> 29
gtaataaata atatgagtta caggctcatg tcattccgtg cgtgtgctgc gaggctctta     60

caacgtctca accacgggat cacgttattt ccactaagtg attggacaaa agtcagatta    120

tttagtggaa agtaacgtga ttagcacgtg aagtcttaca aggttatctt               170


<210> 30
<211> 170
<212> DNA
<213> other sequences


<220> 
<223> ATTG PAM containing guide sequence

<400> 30
gtaataaata atatgagtta caggctcatg tcattccgtg cgtgtgctgc gaggctctta     60

caacgtctca accacgggat cacgttattt ccactaagtg attggacaaa agtcagatta    120

tttagtggaa agtaacgtga ttagcacttt agtgactgta attttctttt               170


<210> 31
<211> 170
<212> DNA
<213> other sequences


<220> 
<223> ATTC PAM containing guide sequence

<400> 31
gtaataaata atatgagtta caggctcatg tcattccgtg cgtgtgctgc gaggctctta     60

caacgtctca accacgggat cacgttattt ccactaagtg attggacaaa agtcagatta    120

tttagtggaa agtaacgtga ttagcacaat cttcaaccct attctgtgaa               170


<210> 32
<211> 170
<212> DNA
<213> other sequences


<220> 
<223> ATTA PAM containing guide sequence

<400> 32
gtaataaata atatgagtta caggctcatg tcattccgtg cgtgtgctgc gaggctctta     60

caacgtctca accacgggat cacgttattt ccactaagtg attggacaaa agtcagatta    120

tttagtggaa agtaacgtga ttagcacata actaaagaaa acaaatctta               170


<210> 33
<211> 170
<212> DNA
<213> other sequences


<220> 
<223> GTTA PAM containing guide sequence

<400> 33
gtaataaata atatgagtta caggctcatg tcattccgtg cgtgtgctgc gaggctctta     60

caacgtctca accacgggat cacgttattt ccactaagtg attggacaaa agtcagatta    120

tttagtggaa agtaacgtga ttagcacgta cagttttgct gaaaggttat               170


<210> 34
<211> 170
<212> DNA
<213> other sequences


<220> 
<223> GTTC PAM containing guide sequence

<400> 34
gtaataaata atatgagtta caggctcatg tcattccgtg cgtgtgctgc gaggctctta     60

caacgtctca accacgggat cacgttattt ccactaagtg attggacaaa agtcagatta    120

tttagtggaa agtaacgtga ttagcactgc ttattatgga caagtagcaa               170


<210> 35
<211> 170
<212> DNA
<213> other sequences


<220> 
<223> GTTT PAM containing guide sequence

<400> 35
gtaataaata atatgagtta caggctcatg tcattccgtg cgtgtgctgc gaggctctta     60

caacgtctca accacgggat cacgttattt ccactaagtg attggacaaa agtcagatta    120

tttagtggaa agtaacgtga ttagcactat tccattaact agcattataa               170


<210> 36
<211> 170
<212> DNA
<213> other sequences


<220> 
<223> GTTT PAM containing guide sequence

<400> 36
gtaataaata atatgagtta caggctcatg tcattccgtg cgtgtgctgc gaggctctta     60

caacgtctca accacgggat cacgttattt ccactaagtg attggacaaa agtcagatta    120

tttagtggaa agtaacgtga ttagcaccag tgcgacgccg cgagccccga               170


<210> 37
<211> 170
<212> DNA
<213> other sequences


<220> 
<223> GTTG PAM containing guide sequence

<400> 37
gtaataaata atatgagtta caggctcatg tcattccgtg cgtgtgctgc gaggctctta     60

caacgtctca accacgggat cacgttattt ccactaagtg attggacaaa agtcagatta    120

tttagtggaa agtaacgtga ttagcacgac gaaaagtttc agtgcgacgc               170


<210> 38
<211> 170
<212> DNA
<213> other sequences


<220> 
<223> GTTC PAM containing guide sequence

<400> 38
gtaataaata atatgagtta caggctcatg tcattccgtg cgtgtgctgc gaggctctta     60

caacgtctca accacgggat cacgttattt ccactaagtg attggacaaa agtcagatta    120

tttagtggaa agtaacgtga ttagcactcg cttcggagga gccgtggtcc               170


<210> 39
<211> 1091
<212> PRT
<213> other sequences


<220> 
<223> independently identified variant of B-Gen.1 with three amino acid
      exchanges

<400> 39
Met Pro Ile Arg Ser Phe Lys Leu Lys Leu Val Thr His Asn Gly Asp 
1               5                   10                  15      
Ser Thr Tyr Met Asp Lys Leu Arg Arg Gly Leu Trp Lys Thr His Val 
            20                  25                  30          
Ile Ile Asn Arg Gly Ile Ala Tyr Tyr Met Asn Thr Leu Ala Leu Met 
        35                  40                  45              
Arg Gln Glu Pro Tyr Gly Ser Lys Ser Arg Glu Glu Val Arg Leu Asp 
    50                  55                  60                  
Leu Leu Ser Thr Leu Arg Glu Gln Gln Arg Arg Asn Asn Trp Ser Glu 
65                  70                  75                  80  
Gln Thr Gly Thr Asp Asp Glu Leu Leu Ser Leu Ser Arg Arg Val Tyr 
                85                  90                  95      
Glu Leu Leu Val Pro Ser Ala Ile Gly Glu Lys Gly Asp Ala Gln Met 
            100                 105                 110         
Leu Ser Arg Lys Phe Leu Ser Pro Leu Val Asp Pro Asn Ser Glu Gly 
        115                 120                 125             
Gly Arg Gly Thr Ala Lys Ser Gly Arg Lys Pro Arg Trp Lys Lys Met 
    130                 135                 140                 
Met Glu Glu Gly His Pro Asp Trp Glu Lys Glu Lys Glu Lys Asp Ala 
145                 150                 155                 160 
Ala Lys Lys Ala Glu Asp Pro Thr Ala Ser Ile Leu Ala Asp Leu Glu 
                165                 170                 175     
Ala Val Gly Leu Leu Pro Leu Phe Pro Leu Phe Ser Asp Glu Gln Lys 
            180                 185                 190         
Glu Ile Arg Trp Leu Pro Lys Lys Lys Arg Gln Phe Val Arg Thr Trp 
        195                 200                 205             
Asp Arg Asp Met Phe Gln Gln Ala Leu Glu Arg Met Leu Ser Trp Glu 
    210                 215                 220                 
Ser Trp Asn Arg Arg Val Ala Glu Glu Tyr Gln Lys Leu Gln Ala Gln 
225                 230                 235                 240 
Arg Asp Glu Val Tyr Ala Lys Tyr Leu Glu Asp Ala Gly Ser Trp Leu 
                245                 250                 255     
Asn Asp Leu Gln Thr Phe Glu Lys Gln Arg Glu Glu Glu Leu Ala Glu 
            260                 265                 270         
Val Ser Phe Glu Pro Asn Ser Glu Tyr Leu Ile Thr Arg Arg Gln Ile 
        275                 280                 285             
Arg Gly Trp Lys Glu Val Tyr Glu Lys Trp Ser Lys Thr Ser Glu Asn 
    290                 295                 300                 
Ala Ser Gln Glu Gln Leu Trp Arg Met Val Ala Asp Val Gln Thr Ala 
305                 310                 315                 320 
Met Ala Gly Ala Phe Gly Asp Pro Lys Val Tyr Gln Phe Leu Ser Gln 
                325                 330                 335     
Pro Lys His His His Ile Trp Arg Glu His Pro Asn Arg Leu Phe Tyr 
            340                 345                 350         
Tyr Ser Lys Tyr Asn Glu Val Arg Glu Lys Leu Asn Arg Ala Lys Lys 
        355                 360                 365             
Gln Ala Ala Phe Thr Leu Pro Asp Pro Val Glu His Pro Leu Trp Thr 
    370                 375                 380                 
Arg Phe Asp Ala Arg Gly Gly Asn Ile His Asp Tyr Glu Ile Ser Lys 
385                 390                 395                 400 
Val Gly Lys Gln Tyr His Val Thr Phe Ser Ser Leu Ile Leu Pro Glu 
                405                 410                 415     
Ala Gln Ser Trp Val Glu Ile Glu Asn Val Thr Val Gly Ile Gly Asn 
            420                 425                 430         
Ser Leu Gln Leu Lys Arg Gln Ile Arg Leu Asp Gly Tyr Ala Asp Lys 
        435                 440                 445             
Lys Gln Lys Val Lys Tyr Tyr Asp Tyr Ser Ser Arg Phe Glu Leu Thr 
    450                 455                 460                 
Gly Val Leu Gly Gly Ala Lys Ile Gln Phe Asp Arg Lys His Leu Lys 
465                 470                 475                 480 
Lys Ala Ala His Arg Leu Ala Glu Gly Glu Thr Gly Pro Ile Phe Leu 
                485                 490                 495     
Asn Val Val Val Asp Val Glu Pro Phe Leu Glu Val Lys Asn Gly Arg 
            500                 505                 510         
Leu Arg Thr Pro Leu Gly Gln Val Leu Gln Val Asn Thr Arg Asp Trp 
        515                 520                 525             
Pro Lys Val Val Asp Tyr Lys Ala Lys Glu Leu Ser Val Leu Met Glu 
    530                 535                 540                 
Asn Thr Gln Ile Gly Asn Glu Asn Gly Val Ser Thr Ile Glu Ala Gly 
545                 550                 555                 560 
Met Arg Ile Met Ser Ile Asp Leu Gly Gln Arg Thr Ala Ala Ala Val 
                565                 570                 575     
Ser Ile Phe Glu Val Ile Ser Lys Lys Pro Asp Glu Lys Glu Thr Lys 
            580                 585                 590         
Leu Phe Tyr Pro Ile Ala Asp Thr Asp Leu Tyr Ala Val His Arg Arg 
        595                 600                 605             
Ser Leu Leu Leu Arg Leu Pro Gly Glu Glu Ile Ser Ser Lys Lys Met 
    610                 615                 620                 
Ile Glu Lys Arg Lys Glu Arg Ala Arg Ile Arg Ser Leu Val Arg Tyr 
625                 630                 635                 640 
Gln Ile Arg Leu Leu Ser Glu Val Leu Arg Leu His Thr Gln Gly Thr 
                645                 650                 655     
Ala Glu Gln Arg Arg Phe Lys Leu Asp Glu Leu Leu Val Ser Ile Gln 
            660                 665                 670         
Arg Lys Leu Glu Leu Asp Gln Ser Glu Trp Ile Ser Glu Leu Glu Lys 
        675                 680                 685             
Leu Phe Asp Tyr Ile Asp Glu Ser Ala Glu Lys Trp Lys Glu Ala Leu 
    690                 695                 700                 
Val Val Ala His Arg Thr Leu Glu Pro Ile Val Val Glu Ala Val Arg 
705                 710                 715                 720 
Asn Trp Lys Lys Ser Leu Ser Lys Glu Asn Lys Asp Arg Arg Arg Ile 
                725                 730                 735     
Ala Gly Ile Ser Ile Trp Ser Ile Glu Glu Leu Glu Glu Thr Arg Lys 
            740                 745                 750         
Leu Leu Ile Ala Trp Ser Lys His Ser Arg Glu Pro Gly Ile Pro Lys 
        755                 760                 765             
Arg Leu Glu Lys Glu Glu Thr Phe Ala Pro Glu His Leu Gln His Ile 
    770                 775                 780                 
Gln Asn Val Lys Asp Asp Arg Leu Lys Gln Met Ala Asn Leu Phe Val 
785                 790                 795                 800 
Met Thr Ala Leu Gly Tyr Lys Tyr Asp Glu Gly Asn Lys Arg Trp Val 
                805                 810                 815     
Glu Ala Tyr Pro Ala Cys Gln Val Ile Leu Phe Glu Asp Leu Ser Arg 
            820                 825                 830         
Tyr Arg Phe Ala Leu Asp Arg Pro Arg Arg Glu Asn Asn Arg Leu Met 
        835                 840                 845             
Lys Trp Ala His Arg Ser Ile Pro Arg Leu Thr Tyr Met Gln Ala Glu 
    850                 855                 860                 
Leu Phe Gly Ile Gln Val Gly Asp Val Tyr Ser Ala Tyr Thr Ser Arg 
865                 870                 875                 880 
Phe His Ala Lys Thr Gly Ala Pro Gly Ile Arg Cys His Ala Leu Thr 
                885                 890                 895     
Glu Ala Asp Leu Gln Ser Asn Ser Tyr Val Val Asn Gln Leu Ile Lys 
            900                 905                 910         
Asp Lys Phe Ile Gln Asp Asn Gln Thr Glu Ile Leu Lys Ala Gly Gln 
        915                 920                 925             
Ile Val Pro Trp Gln Gly Gly Glu Leu Phe Val Thr Phe Ala Asp Arg 
    930                 935                 940                 
Ser Gly Ala Ser Leu Ala Val Ile His Ala Asp Ile Asn Ala Ala Gln 
945                 950                 955                 960 
Asn Leu Gln Lys Arg Phe Trp Gln His Asn Ser Glu Val Phe Arg Val 
                965                 970                 975     
Pro Cys Lys Val Val Lys Gly Gly Leu Val Pro Val Tyr Glu Lys Met 
            980                 985                 990         
Arg Lys Leu Phe Gly Lys Gly Leu Phe Val Asn Ile Asp Asp Pro Glu 
        995                 1000                1005            
Ser Lys Glu Val Tyr Arg Trp Glu His Ser Thr Lys Met Lys Ser Lys 
    1010                1015                1020                
Thr Thr Pro Val Asp Leu Glu Ser Glu Asp Ile Glu His Glu Glu Leu 
1025                1030                1035                1040
Ser Asp Glu Trp Glu Asp Met Gln Glu Gly Tyr Lys Thr Leu Leu Arg 
                1045                1050                1055    
Asp Pro Ser Gly Phe Phe Trp Ser Ser Asp Ser Trp Ile Pro Gln Lys 
            1060                1065                1070        
Asp Phe Trp Ile Arg Val Lys Ser Arg Ile Gly Lys Ser Leu Arg Glu 
        1075                1080                1085            
Gln Ile Arg 
    1090    

<210> 40
<211> 97
<212> RNA
<213> other sequences


<220> 
<223> single guide scaffold with N(23) place holder for a target
      specific sequence B-GEn.1-sgRNA_v4

<400> 40
guuuagcuau aggcuaauaa gauaguugug ucaagugcuu cggagaccua acacgucauc     60

cagucacaac ggcuaucuaa aaauagaugg uuagcac                              97


<210> 41
<211> 87
<212> RNA
<213> other sequences


<220> 
<223> single guide scaffold with N(23) place holder for a target
      specific sequence B-GEn.1-sgRNA_v4.2

<400> 41
uuagcuauag gcuaauaaga uaguuguguc aagugcuucg gagaccuaac acgucaucca     60

gucacaacgg cuaaaaaugg uuagcac                                         87


<210> 42
<211> 87
<212> RNA
<213> other sequences


<220> 
<223> single guide scaffold with N(23) place holder for a target
      specific sequence B-GEn.1-sgRNA_v4.3

<400> 42
uuagcuauag gcuaauaaga uaguuguguc aagugcuucg gagaccuaac acgucaucca     60

gucacaacgg cuaaaaauag ccagcac                                         87


<210> 43
<211> 86
<212> RNA
<213> other sequences


<220> 
<223> single guide scaffold with N(23) place holder for a target
      specific sequence B-GEn.1-sgRNA_v4.4

<400> 43
uuagcuauag gcuaauaaga uaguuguguc aagugcuucg gagaccuaac acgucuccag     60

ucacaacggc uaaaaauagc cagcac                                          86


<210> 44
<211> 86
<212> RNA
<213> other sequences


<220> 
<223> single guide scaffold with N(23) place holder for a target
      specific sequence B-GEn.1-sgRNA_v4.5

<400> 44
uuagcuauag gcuaauaaga uaguugugac aagugcuucg gagaccuaac acgucuccag     60

ucacaacggc uaaaaauagc cagcac                                          86


