                         SEQUENCE LISTING

<110>  BEIJING MEIKANG GENO-IMMUNE BIOTECHNOLOGY CO., LTD.
 
<120>  CD7-BASED HUMANIZED CHIMERIC ANTIGEN RECEPTOR AND USE THEREOF

<130>  22DX0453FPPC

<150>  CN 202110640020.5
<151>  2021-06-08

<150>  CN 202210271824.7
<151>  2022-03-18

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  726
<212>  DNA
<213>  Artificial sequence

<220>
<223>  anti-CD7 scFv

<400>  1
gaagtccaac ttgttgaatc aggaggtggc ttggtaaaac ctgggggctc cctcaaactt       60

agctgcgcgg catccggcct cacttttagc tcttacgcaa tgtcatgggt gagacagaca      120

ccggaaaagc gccttgagtg ggttgcaagc atatcaagtg gtgggttcac atactatcca      180

gacagtgtaa aagggcggtt cacgatttca agagacaacg caagaaacat actgtacctg      240

cagatgagct ctctccgatc cgaagataca gctatgtatt actgcgcgag agatgaggta      300

cgcggttacc ttgatgtctg gggagcgggg actacagtga cagtctccag tggctccact      360

tccggtagcg gcaagcctgg tagttccgag ggcagtacca agggggacat tcagatgacg      420

cagacgactt cctcactttc agccagtctt ggtgatcgcg tcactataag ttgttctgca      480

tcacaaggta tatctaatta cctgaattgg tatcaacaaa agcccgacgg cactgtcaag      540

ttgttgatat attacacctc ctcactccat tctggggtcc cgtcccgatt cagtggtagc      600

gggtctggaa cggactactc cctcaccatt tcaaacttgg agcccgagga tattgctacc      660

tactattgtc agcagtactc aaaactgcct tacacctttg gagggggcac aaaactcgag      720

ataaaa                                                                 726


<210>  2
<211>  242
<212>  PRT
<213>  Artificial sequence

<220>
<223>  anti-CD7 scFv

<400>  2

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Leu Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Ser Ile Ser Ser Gly Gly Phe Thr Tyr Tyr Pro Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Asn Ile Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asp Glu Val Arg Gly Tyr Leu Asp Val Trp Gly Ala Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser 
        115                 120                 125             


Ser Glu Gly Ser Thr Lys Gly Asp Ile Gln Met Thr Gln Thr Thr Ser 
    130                 135                 140                 


Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Ser Ala 
145                 150                 155                 160 


Ser Gln Gly Ile Ser Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp 
                165                 170                 175     


Gly Thr Val Lys Leu Leu Ile Tyr Tyr Thr Ser Ser Leu His Ser Gly 
            180                 185                 190         


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu 
        195                 200                 205             


Thr Ile Ser Asn Leu Glu Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln 
    210                 215                 220                 


Gln Tyr Ser Lys Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys 
        


<210>  3
<211>  1680
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CAR

<400>  3
atgctcctgc ttgtaacgag cctcctgctc tgtgagctgc ctcaccccgc tttccttctt       60

ataccggaag tccaacttgt tgaatcagga ggtggcttgg taaaacctgg gggctccctc      120

aaacttagct gcgcggcatc cggcctcact tttagctctt acgcaatgtc atgggtgaga      180

cagacaccgg aaaagcgcct tgagtgggtt gcaagcatat caagtggtgg gttcacatac      240

tatccagaca gtgtaaaagg gcggttcacg atttcaagag acaacgcaag aaacatactg      300

tacctgcaga tgagctctct ccgatccgaa gatacagcta tgtattactg cgcgagagat      360

gaggtacgcg gttaccttga tgtctgggga gcggggacta cagtgacagt ctccagtggc      420

tccacttccg gtagcggcaa gcctggtagt tccgagggca gtaccaaggg ggacattcag      480

atgacgcaga cgacttcctc actttcagcc agtcttggtg atcgcgtcac tataagttgt      540

tctgcatcac aaggtatatc taattacctg aattggtatc aacaaaagcc cgacggcact      600

gtcaagttgt tgatatatta cacctcctca ctccattctg gggtcccgtc ccgattcagt      660

ggtagcgggt ctggaacgga ctactccctc accatttcaa acttggagcc cgaggatatt      720

gctacctact attgtcagca gtactcaaaa ctgccttaca cctttggagg gggcacaaaa      780

ctcgagataa aagcggccgc aattgaagtt atgtatcctc ctccttacct agacaatgag      840

aagagcaatg gaaccattat ccatgtgaaa gggaaacacc tttgtccaag tcccctattt      900

cccggacctt ctaagccctt ttgggtgctg gtggtggttg ggggagtcct ggcttgctat      960

agcttgctag taacagtggc ctttattatt ttctgggtga ggagtaagag gagcaggctc     1020

ctgcacagtg actacatgaa catgactccc cgccgccctg ggcccacccg caagcattac     1080

cagccctatg ccccaccacg cgacttcgca gcctatcgct ccgctagcgg cggaggtggc     1140

tctggcggtg gcggatcagt tgttaaacgg ggcagaaaga aactcctgta tatattcaaa     1200

caaccattta tgagaccagt acaaactact caagaggaag atggctgtag ctgccgattt     1260

ccagaagaag aagaaggagg atgtgaactg ggcggcggcg gcagcggagg cggtggcagc     1320

ggcggcggcg gctctagagt gaagttcagc aggagcgcag acgcccccgc gtaccagcag     1380

ggccagaacc agctctataa cgagctcaat ctaggacgaa gagaggagta cgatgttttg     1440

gacaagagac gtggccggga ccctgagatg gggggaaagc cgagaaggaa gaaccctcag     1500

gaaggcctgt acaatgaact gcagaaagat aagatggcgg aggcctacag tgagattggg     1560

atgaaaggcg agcgccggag gggcaagggg cacgatggcc tttaccaggg tctcagtaca     1620

gccaccaagg acacctacga cgcccttcac atgcaggccc tgccccctcg cactagttaa     1680


<210>  4
<211>  2946
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CAR

<400>  4
atgctcctgc ttgtaacgag cctcctgctc tgtgagctgc ctcaccccgc tttccttctt       60

ataccggaag tccaacttgt tgaatcagga ggtggcttgg taaaacctgg gggctccctc      120

aaacttagct gcgcggcatc cggcctcact tttagctctt acgcaatgtc atgggtgaga      180

cagacaccgg aaaagcgcct tgagtgggtt gcaagcatat caagtggtgg gttcacatac      240

tatccagaca gtgtaaaagg gcggttcacg atttcaagag acaacgcaag aaacatactg      300

tacctgcaga tgagctctct ccgatccgaa gatacagcta tgtattactg cgcgagagat      360

gaggtacgcg gttaccttga tgtctgggga gcggggacta cagtgacagt ctccagtggc      420

tccacttccg gtagcggcaa gcctggtagt tccgagggca gtaccaaggg ggacattcag      480

atgacgcaga cgacttcctc actttcagcc agtcttggtg atcgcgtcac tataagttgt      540

tctgcatcac aaggtatatc taattacctg aattggtatc aacaaaagcc cgacggcact      600

gtcaagttgt tgatatatta cacctcctca ctccattctg gggtcccgtc ccgattcagt      660

ggtagcgggt ctggaacgga ctactccctc accatttcaa acttggagcc cgaggatatt      720

gctacctact attgtcagca gtactcaaaa ctgccttaca cctttggagg gggcacaaaa      780

ctcgagataa aagcggccgc aattgaagtt atgtatcctc ctccttacct agacaatgag      840

aagagcaatg gaaccattat ccatgtgaaa gggaaacacc tttgtccaag tcccctattt      900

cccggacctt ctaagccctt ttgggtgctg gtggtggttg ggggagtcct ggcttgctat      960

agcttgctag taacagtggc ctttattatt ttctgggtga ggagtaagag gagcaggctc     1020

ctgcacagtg actacatgaa catgactccc cgccgccctg ggcccacccg caagcattac     1080

cagccctatg ccccaccacg cgacttcgca gcctatcgct ccgctagcgg aggtggaggt     1140

tctggaggtg gtggaagtca aagaaggaag taccgcagca acaaaggaga atctcccgtc     1200

gagccagccg agccctgtca ttattcatgc ccaagggagg aggagggaag tacaatccca     1260

attcaagaag actacaggaa gcccgaacct gcatgcagtc caggtggagg cggttctgga     1320

ggcggtggct cccgggtgaa attctcacgg tctgcagacg cacccgctta ccagcaaggc     1380

cagaaccaac tctataacga gctcaatcta ggacgaagag aggagtacga tgttttggac     1440

aagagacgtg gccgggaccc tgagatgggg ggaaagccga gaaggaagaa ccctcaggaa     1500

ggcctgtaca atgaactgca gaaagataag atggcggagg cctacagtga gattgggatg     1560

aaaggcgagc gccggagggg caaggggcac gatggccttt accagggtct cagtacagcc     1620

accaaggaca cctacgacgc ccttcacatg caggccctgc cccctcgcac tagtggctcc     1680

ggagccacga acttctctct gttaaagcaa gcaggagacg tggaagaaaa ccccggtccc     1740

atgggagtgc aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc     1800

cagacctgcg tggtgcacta caccgggatg cttgaagatg gaaagaaagt ggactcctcc     1860

cgggacagaa acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg     1920

gaagaagggg ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat     1980

tatgcctatg gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc     2040

gatgtggagc ttctaaaact ggaaggtgga ggcggttcag gcggcggcgg cagcggcgcc     2100

atggtcggtg ctcttgagag tttgagggga aatgcagatt tggcttacat cctgagcatg     2160

gagccctgtg gccactgcct cattatcaac aatgtgaact tctgccgtga gtccgggctc     2220

cgcacccgca ctggctccaa catcgactgt gagaagttgc ggcgtcgctt ctcctcgctg     2280

catttcatgg tggaggtgaa gggcgacctg actgccaaga aaatggtgct ggctttgctg     2340

gagctggcgc ggcaggacca cggtgctctg gactgctgcg tggtggtcat tctctctcac     2400

ggctgtcagg ccagccacct gcagttccca ggggctgtct acggcacaga tggatgccct     2460

gtgtcggtcg agaagattgt gaacatcttc aatgggacca gctgccccag cctgggaggg     2520

aagcccaagc tctttttcat ccaggcctgt ggtggggagc agaaagacca tgggtttgag     2580

gtggcctcca cttcccctga agacgagtcc cctggcagta accccgagcc agatgccacc     2640

ccgttccagg aaggtttgag gaccttcgac cagctggacg ccatatctag tttgcccaca     2700

cccagtgaca tctttgtgtc ctactctact ttcccaggtt ttgtttcctg gagggacccc     2760

aagagtggct cctggtacgt tgagaccctg gacgacatct ttgagcagtg ggctcactct     2820

gaagacctgc agtccctcct gcttagggtc gctaatgctg tttcggtgaa agggatttat     2880

aaacagatgc ctggttgctt taatttcctc cggaaaaaac ttttctttaa aacatcagct     2940

agttaa                                                                2946


<210>  5
<211>  1644
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CAR

<400>  5
atgctcctgc ttgtaacgag cctcctgctc tgtgagctgc ctcaccccgc tttccttctt       60

ataccggaag tccaacttgt tgaatcagga ggtggcttgg taaaacctgg gggctccctc      120

aaacttagct gcgcggcatc cggcctcact tttagctctt acgcaatgtc atgggtgaga      180

cagacaccgg aaaagcgcct tgagtgggtt gcaagcatat caagtggtgg gttcacatac      240

tatccagaca gtgtaaaagg gcggttcacg atttcaagag acaacgcaag aaacatactg      300

tacctgcaga tgagctctct ccgatccgaa gatacagcta tgtattactg cgcgagagat      360

gaggtacgcg gttaccttga tgtctgggga gcggggacta cagtgacagt ctccagtggc      420

tccacttccg gtagcggcaa gcctggtagt tccgagggca gtaccaaggg ggacattcag      480

atgacgcaga cgacttcctc actttcagcc agtcttggtg atcgcgtcac tataagttgt      540

tctgcatcac aaggtatatc taattacctg aattggtatc aacaaaagcc cgacggcact      600

gtcaagttgt tgatatatta cacctcctca ctccattctg gggtcccgtc ccgattcagt      660

ggtagcgggt ctggaacgga ctactccctc accatttcaa acttggagcc cgaggatatt      720

gctacctact attgtcagca gtactcaaaa ctgccttaca cctttggagg gggcacaaaa      780

ctcgagataa aagcggccgc aattgaagtt atgtatcctc ctccttacct agacaatgag      840

aagagcaatg gaaccattat ccatgtgaaa gggaaacacc tttgtccaag tcccctattt      900

cccggacctt ctaagccctt ttgggtgctg gtggtggttg ggggagtcct ggcttgctat      960

agcttgctag taacagtggc ctttattatt ttctgggtga ggagtaagag gagcaggctc     1020

ctgcacagtg actacatgaa catgactccc cgccgccctg ggcccacccg caagcattac     1080

cagccctatg ccccaccacg cgacttcgca gcctatcgct ccgctagcgg tggaggtgga     1140

tctggtggtg gaggttccaa atctcggcag acaccacctc tcgcatctgt cgagatggag     1200

gccatggagg cactgcctgt cacatggggt acttccagca gggacgagga cctcgaaaat     1260

tgctcccatc atctgggtgg cggcggttcc ggtggtggag gttctcgggt gaaattctcc     1320

aggtccgcag acgctccagc ctaccagcaa ggacagaacc aactgtacaa tgagctgaat     1380

ctgggaagac gcgaggagta tgacgtgctg gacaaacgga gaggtcgcga ccccgagatg     1440

ggaggcaagc ctcgcaggaa gaacccacag gaaggactgt acaacgaact gcagaaggac     1500

aagatggccg aggcatactc agagatcgga atgaagggcg agagacgcag agggaaaggc     1560

cacgacggac tgtaccaggg actgtcaact gccacaaagg atacttacga tgccctgcac     1620

atgcaggcac tccctccacg ctga                                            1644


<210>  6
<211>  559
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CAR

<400>  6

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Leu Thr Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Thr Pro Glu 
    50                  55                  60                  


Lys Arg Leu Glu Trp Val Ala Ser Ile Ser Ser Gly Gly Phe Thr Tyr 
65                  70                  75                  80  


Tyr Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                85                  90                  95      


Arg Asn Ile Leu Tyr Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr 
            100                 105                 110         


Ala Met Tyr Tyr Cys Ala Arg Asp Glu Val Arg Gly Tyr Leu Asp Val 
        115                 120                 125             


Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ser Gly Ser Thr Ser Gly 
    130                 135                 140                 


Ser Gly Lys Pro Gly Ser Ser Glu Gly Ser Thr Lys Gly Asp Ile Gln 
145                 150                 155                 160 


Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val 
                165                 170                 175     


Thr Ile Ser Cys Ser Ala Ser Gln Gly Ile Ser Asn Tyr Leu Asn Trp 
            180                 185                 190         


Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr Tyr Thr 
        195                 200                 205             


Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser 
    210                 215                 220                 


Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Pro Glu Asp Ile 
225                 230                 235                 240 


Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Lys Leu Pro Tyr Thr Phe Gly 
                245                 250                 255     


Gly Gly Thr Lys Leu Glu Ile Lys Ala Ala Ala Ile Glu Val Met Tyr 
            260                 265                 270         


Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile His 
        275                 280                 285             


Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser 
    290                 295                 300                 


Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr 
305                 310                 315                 320 


Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys 
                325                 330                 335     


Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg 
            340                 345                 350         


Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp 
        355                 360                 365             


Phe Ala Ala Tyr Arg Ser Ala Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    370                 375                 380                 


Gly Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys 
385                 390                 395                 400 


Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys 
                405                 410                 415     


Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly Gly 
            420                 425                 430         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Arg Val Lys 
        435                 440                 445             


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
    450                 455                 460                 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
465                 470                 475                 480 


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
                485                 490                 495     


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
            500                 505                 510         


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
        515                 520                 525             


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
    530                 535                 540                 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Thr Ser 
545                 550                 555                 


<210>  7
<211>  981
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CAR

<400>  7

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Leu Thr Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Thr Pro Glu 
    50                  55                  60                  


Lys Arg Leu Glu Trp Val Ala Ser Ile Ser Ser Gly Gly Phe Thr Tyr 
65                  70                  75                  80  


Tyr Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                85                  90                  95      


Arg Asn Ile Leu Tyr Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr 
            100                 105                 110         


Ala Met Tyr Tyr Cys Ala Arg Asp Glu Val Arg Gly Tyr Leu Asp Val 
        115                 120                 125             


Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ser Gly Ser Thr Ser Gly 
    130                 135                 140                 


Ser Gly Lys Pro Gly Ser Ser Glu Gly Ser Thr Lys Gly Asp Ile Gln 
145                 150                 155                 160 


Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val 
                165                 170                 175     


Thr Ile Ser Cys Ser Ala Ser Gln Gly Ile Ser Asn Tyr Leu Asn Trp 
            180                 185                 190         


Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr Tyr Thr 
        195                 200                 205             


Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser 
    210                 215                 220                 


Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Pro Glu Asp Ile 
225                 230                 235                 240 


Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Lys Leu Pro Tyr Thr Phe Gly 
                245                 250                 255     


Gly Gly Thr Lys Leu Glu Ile Lys Ala Ala Ala Ile Glu Val Met Tyr 
            260                 265                 270         


Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile His 
        275                 280                 285             


Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser 
    290                 295                 300                 


Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr 
305                 310                 315                 320 


Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys 
                325                 330                 335     


Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg 
            340                 345                 350         


Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp 
        355                 360                 365             


Phe Ala Ala Tyr Arg Ser Ala Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    370                 375                 380                 


Gly Ser Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro Val 
385                 390                 395                 400 


Glu Pro Ala Glu Pro Cys His Tyr Ser Cys Pro Arg Glu Glu Glu Gly 
                405                 410                 415     


Ser Thr Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala Cys 
            420                 425                 430         


Ser Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Arg Val Lys Phe 
        435                 440                 445             


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
    450                 455                 460                 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
465                 470                 475                 480 


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
                485                 490                 495     


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
            500                 505                 510         


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
        515                 520                 525             


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
    530                 535                 540                 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Thr Ser Gly Ser 
545                 550                 555                 560 


Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu 
                565                 570                 575     


Asn Pro Gly Pro Met Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp 
            580                 585                 590         


Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr 
        595                 600                 605             


Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn 
    610                 615                 620                 


Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp 
625                 630                 635                 640 


Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr 
                645                 650                 655     


Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile 
            660                 665                 670         


Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
        675                 680                 685             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ala Met Val Gly Ala 
    690                 695                 700                 


Leu Glu Ser Leu Arg Gly Asn Ala Asp Leu Ala Tyr Ile Leu Ser Met 
705                 710                 715                 720 


Glu Pro Cys Gly His Cys Leu Ile Ile Asn Asn Val Asn Phe Cys Arg 
                725                 730                 735     


Glu Ser Gly Leu Arg Thr Arg Thr Gly Ser Asn Ile Asp Cys Glu Lys 
            740                 745                 750         


Leu Arg Arg Arg Phe Ser Ser Leu His Phe Met Val Glu Val Lys Gly 
        755                 760                 765             


Asp Leu Thr Ala Lys Lys Met Val Leu Ala Leu Leu Glu Leu Ala Arg 
    770                 775                 780                 


Gln Asp His Gly Ala Leu Asp Cys Cys Val Val Val Ile Leu Ser His 
785                 790                 795                 800 


Gly Cys Gln Ala Ser His Leu Gln Phe Pro Gly Ala Val Tyr Gly Thr 
                805                 810                 815     


Asp Gly Cys Pro Val Ser Val Glu Lys Ile Val Asn Ile Phe Asn Gly 
            820                 825                 830         


Thr Ser Cys Pro Ser Leu Gly Gly Lys Pro Lys Leu Phe Phe Ile Gln 
        835                 840                 845             


Ala Cys Gly Gly Glu Gln Lys Asp His Gly Phe Glu Val Ala Ser Thr 
    850                 855                 860                 


Ser Pro Glu Asp Glu Ser Pro Gly Ser Asn Pro Glu Pro Asp Ala Thr 
865                 870                 875                 880 


Pro Phe Gln Glu Gly Leu Arg Thr Phe Asp Gln Leu Asp Ala Ile Ser 
                885                 890                 895     


Ser Leu Pro Thr Pro Ser Asp Ile Phe Val Ser Tyr Ser Thr Phe Pro 
            900                 905                 910         


Gly Phe Val Ser Trp Arg Asp Pro Lys Ser Gly Ser Trp Tyr Val Glu 
        915                 920                 925             


Thr Leu Asp Asp Ile Phe Glu Gln Trp Ala His Ser Glu Asp Leu Gln 
    930                 935                 940                 


Ser Leu Leu Leu Arg Val Ala Asn Ala Val Ser Val Lys Gly Ile Tyr 
945                 950                 955                 960 


Lys Gln Met Pro Gly Cys Phe Asn Phe Leu Arg Lys Lys Leu Phe Phe 
                965                 970                 975     


Lys Thr Ser Ala Ser 
            980     


<210>  8
<211>  547
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CAR

<400>  8

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Leu Thr Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Thr Pro Glu 
    50                  55                  60                  


Lys Arg Leu Glu Trp Val Ala Ser Ile Ser Ser Gly Gly Phe Thr Tyr 
65                  70                  75                  80  


Tyr Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                85                  90                  95      


Arg Asn Ile Leu Tyr Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr 
            100                 105                 110         


Ala Met Tyr Tyr Cys Ala Arg Asp Glu Val Arg Gly Tyr Leu Asp Val 
        115                 120                 125             


Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ser Gly Ser Thr Ser Gly 
    130                 135                 140                 


Ser Gly Lys Pro Gly Ser Ser Glu Gly Ser Thr Lys Gly Asp Ile Gln 
145                 150                 155                 160 


Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val 
                165                 170                 175     


Thr Ile Ser Cys Ser Ala Ser Gln Gly Ile Ser Asn Tyr Leu Asn Trp 
            180                 185                 190         


Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr Tyr Thr 
        195                 200                 205             


Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser 
    210                 215                 220                 


Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Pro Glu Asp Ile 
225                 230                 235                 240 


Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Lys Leu Pro Tyr Thr Phe Gly 
                245                 250                 255     


Gly Gly Thr Lys Leu Glu Ile Lys Ala Ala Ala Ile Glu Val Met Tyr 
            260                 265                 270         


Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile His 
        275                 280                 285             


Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser 
    290                 295                 300                 


Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr 
305                 310                 315                 320 


Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys 
                325                 330                 335     


Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg 
            340                 345                 350         


Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp 
        355                 360                 365             


Phe Ala Ala Tyr Arg Ser Ala Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    370                 375                 380                 


Gly Ser Lys Ser Arg Gln Thr Pro Pro Leu Ala Ser Val Glu Met Glu 
385                 390                 395                 400 


Ala Met Glu Ala Leu Pro Val Thr Trp Gly Thr Ser Ser Arg Asp Glu 
                405                 410                 415     


Asp Leu Glu Asn Cys Ser His His Leu Gly Gly Gly Gly Ser Gly Gly 
            420                 425                 430         


Gly Gly Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
        435                 440                 445             


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
    450                 455                 460                 


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
465                 470                 475                 480 


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
                485                 490                 495     


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
            500                 505                 510         


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
        515                 520                 525             


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
    530                 535                 540                 


Pro Pro Arg 
545         


<210>  9
<211>  1695
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CAR

<400>  9
atgctcctgc ttgtgacctc actcttgctt tgtgaactgc cacatccggc ctttctgttg       60

atcccgcaag tccaacttgt tgagtcagga ggcggtctcg tgcagccagg tggatcacgc      120

aaactttctt gtgcagcttc cggattcaca ttcagtagtt tcgggatgca ttgggtcagg      180

caagctccag agaaagggct cgaatgggta gcgtatatct ctagtggatc tagtacgctg      240

cattacgctg acaccgtcaa aggtcggttt acgattagca gggataaccc caagaatacg      300

ctgttcttgc agatgacatc attgcggtct gaagatacgg ccatgtacta ctgcgcccgg      360

tgggggaact atccccatta tgcgatggat tactggggtc aaggcacctc tgtcacggtt      420

tcttccggaa gcacgagtgg gagtgggaaa cctggcagct ctgaggggag tacgaaaggt      480

gatatagtga tgacacaaag ccccgcctca cttgcagtgt ctcttgggga cagagcgacc      540

atcagttgcc gagccagcaa atctgtttct gcctctggat acagttatat gcactggtat      600

caacagaaac cggggcaacc gccaaagttg ctcatatact tggcgtcaaa tctcgaatcc      660

ggtgttcctg cacggttctc cggctctgga tctggtacag atttcactct caacatccat      720

cccgtcgagg aggaggacgc tgtaacctac tactgtcagc attccaggga gcttccttac      780

acatttggcg gcggcacaaa gctcgagatt aaggcggccg caattgaagt tatgtatcct      840

cctccttacc tagacaatga gaagagcaat ggaaccatta tccatgtgaa agggaaacac      900

ctttgtccaa gtcccctatt tcccggacct tctaagccct tttgggtgct ggtggtggtt      960

gggggagtcc tggcttgcta tagcttgcta gtaacagtgg cctttattat tttctgggtg     1020

aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgccct     1080

gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc     1140

tccgctagcg gaggtggagg ttctggaggt ggtggaagtc aaagaaggaa gtaccgcagc     1200

aacaaaggag aatctcccgt cgagccagcc gagccctgtc attattcatg cccaagggag     1260

gaggagggaa gtacaatccc aattcaagaa gactacagga agcccgaacc tgcatgcagt     1320

ccaggtggag gcggttctgg aggcggtggc tcccgggtga aattctcacg gtctgcagac     1380

gcacccgctt accagcaagg ccagaaccaa ctctataacg agctcaatct aggacgaaga     1440

gaggagtacg atgttttgga caagagacgt ggccgggacc ctgagatggg gggaaagccg     1500

agaaggaaga accctcagga aggcctgtac aatgaactgc agaaagataa gatggcggag     1560

gcctacagtg agattgggat gaaaggcgag cgccggaggg gcaaggggca cgatggcctt     1620

taccagggtc tcagtacagc caccaaggac acctacgacg cccttcacat gcaggccctg     1680

ccccctcgca ctagt                                                      1695


<210>  10
<211>  1701
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CAR

<400>  10
atgctcctgc ttgtgacctc actcttgctt tgtgaactgc cacatccggc ctttctgttg       60

atcccgcaag tccaacttgt tgagtcagga ggcggtctcg tgcagccagg tggatcacgc      120

aaactttctt gtgcagcttc cggattcaca ttcagtagtt tcgggatgca ttgggtcagg      180

caagctccag agaaagggct cgaatgggta gcgtatatct ctagtggatc tagtacgctg      240

cattacgctg acaccgtcaa aggtcggttt acgattagca gggataaccc caagaatacg      300

ctgttcttgc agatgacatc attgcggtct gaagatacgg ccatgtacta ctgcgcccgg      360

tgggggaact atccccatta tgcgatggat tactggggtc aaggcacctc tgtcacggtt      420

tcttccggaa gcacgagtgg gagtgggaaa cctggcagct ctgaggggag tacgaaaggt      480

gatatagtga tgacacaaag ccccgcctca cttgcagtgt ctcttgggga cagagcgacc      540

atcagttgcc gagccagcaa atctgtttct gcctctggat acagttatat gcactggtat      600

caacagaaac cggggcaacc gccaaagttg ctcatatact tggcgtcaaa tctcgaatcc      660

ggtgttcctg cacggttctc cggctctgga tctggtacag atttcactct caacatccat      720

cccgtcgagg aggaggacgc tgtaacctac tactgtcagc attccaggga gcttccttac      780

acatttggcg gcggcacaaa gctcgagatt aaggcggccg caattgaagt tatgtatcct      840

cctccttacc tagacaatga gaagagcaat ggaaccatta tccatgtgaa agggaaacac      900

ctttgtccaa gtcccctatt tcccggacct tctaagccct tttgggtgct ggtggtggtt      960

gggggagtcc tggcttgcta tagcttgcta gtaacagtgg cctttattat tttctgggtg     1020

aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgccct     1080

gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc     1140

tccgctagcg gcggaggtgg ctctggcggt ggcggatcag ttgttaaacg gggcagaaag     1200

aaactcctgt atatattcaa acaaccattt atgagaccag tacaaactac tcaagaggaa     1260

gatggctgta gctgccgatt tccagaagaa gaagaaggag gatgtgaact gggcggcggc     1320

ggcagcggag gcggtggcag cggcggcggc ggctctagag tgaagttcag caggagcgca     1380

gacgcccccg cgtaccagca gggccagaac cagctctata acgagctcaa tctaggacga     1440

agagaggagt acgatgtttt ggacaagaga cgtggccggg accctgagat ggggggaaag     1500

ccgagaagga agaaccctca ggaaggcctg tacaatgaac tgcagaaaga taagatggcg     1560

gaggcctaca gtgagattgg gatgaaaggc gagcgccgga ggggcaaggg gcacgatggc     1620

ctttaccagg gtctcagtac agccaccaag gacacctacg acgcccttca catgcaggcc     1680

ctgccccctc gcactagtta a                                               1701


<210>  11
<211>  21
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CD8a signal peptide

<400>  11

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  12
<211>  22
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GM-CSFR signal peptide

<400>  12

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro 
            20          


<210>  13
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hinge

<400>  13

Gly Gly Gly Gly Ser 
1               5   


<210>  14
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hinge

<400>  14

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


