                         SEQUENCE LISTING

<110>  Beijing Mabworks Biotech Co., Ltd
       
<120>  ANTIBODIES BINDING TIGIT AND USES THEREOF

<130>  55556 00059

<150>  CN202110650525.X
<151>  2021-06-10

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR1 of mouse, chimeric and humanized 70E11 antibodies

<400>  1

Ser Tyr Asn Val His 
1               5   


<210>  2
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR2 of mouse, chimeric and humanized 70E11 antibodies

<400>  2

Thr Ile Tyr Pro Gly Asn Leu Ala Thr Ser Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR3 of mouse, chimeric and humanized 70E11 antibodies

<400>  3

Ser Gly Thr Met Asp Tyr 
1               5       


<210>  4
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR1 of mouse, chimeric and humanized 70E11 antibodies

<400>  4

Arg Ala Ser Ser Ser Ile Ser Ser Thr Tyr Leu His 
1               5                   10          


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR2 of mouse, chimeric and humanized 70E11 antibodies

<400>  5

Asn Thr Gln Asn Leu Ala Ser 
1               5           


<210>  6
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR3 of mouse, chimeric and humanized 70E11 antibodies

<400>  6

Gln Gln Phe Gly Gly Tyr Pro Leu Ile Thr 
1               5                   10  


<210>  7
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR1 of mouse, chimeric and humanized 149G11 antibodies

<400>  7

Asn Tyr Trp Ile His 
1               5   


<210>  8
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR2 of mouse, chimeric and humanized 149G11 antibodies

<400>  8

Asp Ile Tyr Pro Gly Gly Arg Tyr Ser Asn Tyr Asn Glu Lys Phe Arg 
1               5                   10                  15      


Gly 
    


<210>  9
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR3 of mouse, chimeric and humanized 149G11 antibodies

<400>  9

Tyr Tyr Glu Ser Ala Met Asp Phe 
1               5               


<210>  10
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR1 of mouse, chimeric and humanized 149G11 antibodies

<400>  10

Lys Ser Ser Gln Asn Leu Leu Tyr Asn Ser Asn Gln Lys Ser Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR2 of mouse, chimeric and humanized 149G11 antibodies

<400>  11

Trp Ala Ser Thr Arg Glu Ser 
1               5           


<210>  12
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR3 of mouse, chimeric and humanized 149G11 antibodies

<400>  12

Gln Gln Tyr Tyr Asn Tyr Pro Phe Thr 
1               5                   


<210>  13
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of mouse and chimeric 70E11 antibodies 

<400>  13

Gln Val Gln Leu Gln Gln Pro Gly Thr Asp Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Arg Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Val His Trp Val Lys Gln Thr Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Thr Ile Tyr Pro Gly Asn Leu Ala Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Thr Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Thr Met Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  14
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of 70E11-VH1VL1

<400>  14

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Val His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Thr Ile Tyr Pro Gly Asn Leu Ala Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Thr Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  15
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of 70E11-VH2VL2, 70E11-VH2VL3 and 70E11-VH2VL4

<400>  15

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Val His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Thr Ile Tyr Pro Gly Asn Leu Ala Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Leu Thr Ala Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Thr Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  16
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of 70E11-VH3VL2, 70E11-VH3VL3 and 70E11-VH3VL4

<400>  16

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Val His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Thr Ile Tyr Pro Gly Asn Leu Ala Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Leu Thr Ala Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Thr Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  17
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of 70E11-VH4VL2, 70E11-VH4VL3 and 70E11-VH4VL4

<400>  17

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Val His Trp Val Lys Gln Thr Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Thr Ile Tyr Pro Gly Asn Leu Ala Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Leu Thr Ala Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Thr Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  18
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of 70E11-VH5VL2, 70E11-VH5VL3 and 70E11-VH5VL4

<400>  18

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Val His Trp Val Lys Gln Thr Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Thr Ile Tyr Pro Gly Asn Leu Ala Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Thr Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  19
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of mouse and chimeric 149G11 antibodies

<400>  19

Gln Val Gln Leu Gln Gln Ser Gly Thr Glu Leu Val Arg Pro Gly Thr 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Arg Thr Ala Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Val Lys Gln Arg Pro Gly His Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asp Ile Tyr Pro Gly Gly Arg Tyr Ser Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Arg Gly Lys Ala Thr Leu Thr Ala Asp Thr Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Glu Ser Ala Met Asp Phe Trp Gly Gln Gly Thr Ser 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  20
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of 149G11-VH1VL1

<400>  20

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Asp Ile Tyr Pro Gly Gly Arg Tyr Ser Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Arg Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Glu Ser Ala Met Asp Phe Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  21
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of 149G11-VH2VL2 and 149G11-VH2VL3

<400>  21

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asp Ile Tyr Pro Gly Gly Arg Tyr Ser Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Arg Gly Arg Val Thr Leu Thr Ala Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Glu Ser Ala Met Asp Phe Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  22
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of 149G11-VH3VL2 and 149G11-VH3VL3

<400>  22

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Val Lys Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asp Ile Tyr Pro Gly Gly Arg Tyr Ser Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Arg Gly Arg Val Thr Leu Thr Ala Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Glu Ser Ala Met Asp Phe Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  23
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of 149G11-VH4VL2 and 149G11-VH4VL3

<400>  23

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Val Lys Gln Arg Pro Gly His Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asp Ile Tyr Pro Gly Gly Arg Tyr Ser Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Arg Gly Arg Val Thr Leu Thr Ala Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Glu Ser Ala Met Asp Phe Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  24
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of mouse and chimeric 70E11 antibodies

<400>  24

Glu Asn Val Val Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Ile Ser Ser Thr 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Ser Gly Ala Ser Pro Lys Leu Trp 
        35                  40                  45              


Ile Tyr Asn Thr Gln Asn Leu Ala Ser Gly Val Pro Ala Arg Ile Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Val Glu 
65                  70                  75                  80  


Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Phe Gly Gly Tyr Pro 
                85                  90                  95      


Leu Ile Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 


<210>  25
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of 70E11-VH1VL1

<400>  25

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Ser Ser Ile Ser Ser Thr 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Asn Thr Gln Asn Leu Ala Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Phe Gly Gly Tyr Pro 
                85                  90                  95      


Leu Ile Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 


<210>  26
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of 70E11-VH2VL2, 70E11-VH3VL2, 70E11-VH4VL2 and 70E11-VH5VL2

<400>  26

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Ser Ser Ile Ser Ser Thr 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Asn Thr Gln Asn Leu Ala Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Ser Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Phe Gly Gly Tyr Pro 
                85                  90                  95      


Leu Ile Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 


<210>  27
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of 70E11-VH2VL3, 70E11-VH3VL3, 70E11-VH4VL3 and 70E11-VH5VL3

<400>  27

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Ser Ser Ile Ser Ser Thr 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Ala Ser Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Asn Thr Gln Asn Leu Ala Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Ser Tyr Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Phe Gly Gly Tyr Pro 
                85                  90                  95      


Leu Ile Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 


<210>  28
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of 70E11-VH2VL4, 70E11-VH3VL4, 70E11-VH4VL4 and 70E11-VH5VL4

<400>  28

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Met Thr Cys Arg Ala Ser Ser Ser Ile Ser Ser Thr 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Ala Ser Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Asn Thr Gln Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Ser Tyr Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Phe Gly Gly Tyr Pro 
                85                  90                  95      


Leu Ile Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 


<210>  29
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of mouse and chimeric 149G11 antibodies

<400>  29

Asp Ile Val Met Ser Gln Ser Pro Ser Ser Leu Ala Val Ser Val Gly 
1               5                   10                  15      


Glu Lys Val Thr Val Asn Cys Lys Ser Ser Gln Asn Leu Leu Tyr Asn 
            20                  25                  30          


Ser Asn Gln Lys Ser Tyr Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Lys Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asn Tyr Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys 
    


<210>  30
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of 149G11-VH1VL1

<400>  30

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Asn Leu Leu Tyr Asn 
            20                  25                  30          


Ser Asn Gln Lys Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asn Tyr Pro Phe Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
            100                 105                 110         


Lys 
    


<210>  31
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of 149G11-VH2VL2, 149G11-VH3VL2 and 149G11-VH4VL2

<400>  31

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Asn Leu Leu Tyr Asn 
            20                  25                  30          


Ser Asn Gln Lys Ser Tyr Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asn Tyr Pro Phe Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
            100                 105                 110         


Lys 
    


<210>  32
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of 149G11-VH2VL3, 149G11-VH3VL3 and 149G11-VH4VL3

<400>  32

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Val Asn Cys Lys Ser Ser Gln Asn Leu Leu Tyr Asn 
            20                  25                  30          


Ser Asn Gln Lys Ser Tyr Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asn Tyr Pro Phe Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
            100                 105                 110         


Lys 
    


<210>  33
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 heavy chain constant region

<400>  33

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  34
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human  light chain constant region

<400>  34

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  35
<211>  244
<212>  PRT
<213>  Homo sapiens

<400>  35

Met Arg Trp Cys Leu Leu Leu Ile Trp Ala Gln Gly Leu Arg Gln Ala 
1               5                   10                  15      


Pro Leu Ala Ser Gly Met Met Thr Gly Thr Ile Glu Thr Thr Gly Asn 
            20                  25                  30          


Ile Ser Ala Glu Lys Gly Gly Ser Ile Ile Leu Gln Cys His Leu Ser 
        35                  40                  45              


Ser Thr Thr Ala Gln Val Thr Gln Val Asn Trp Glu Gln Gln Asp Gln 
    50                  55                  60                  


Leu Leu Ala Ile Cys Asn Ala Asp Leu Gly Trp His Ile Ser Pro Ser 
65                  70                  75                  80  


Phe Lys Asp Arg Val Ala Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln 
                85                  90                  95      


Ser Leu Thr Val Asn Asp Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr 
            100                 105                 110         


Tyr Pro Asp Gly Thr Tyr Thr Gly Arg Ile Phe Leu Glu Val Leu Glu 
        115                 120                 125             


Ser Ser Val Ala Glu His Gly Ala Arg Phe Gln Ile Pro Leu Leu Gly 
    130                 135                 140                 


Ala Met Ala Ala Thr Leu Val Val Ile Cys Thr Ala Val Ile Val Val 
145                 150                 155                 160 


Val Ala Leu Thr Arg Lys Lys Lys Ala Leu Arg Ile His Ser Val Glu 
                165                 170                 175     


Gly Asp Leu Arg Arg Lys Ser Ala Gly Gln Glu Glu Trp Ser Pro Ser 
            180                 185                 190         


Ala Pro Ser Pro Pro Gly Ser Cys Val Gln Ala Glu Ala Ala Pro Ala 
        195                 200                 205             


Gly Leu Cys Gly Glu Gln Arg Gly Glu Asp Cys Ala Glu Leu His Asp 
    210                 215                 220                 


Tyr Phe Asn Val Leu Ser Tyr Arg Ser Leu Gly Asn Cys Ser Phe Phe 
225                 230                 235                 240 


Thr Glu Thr Gly 
                


<210>  36
<211>  312
<212>  PRT
<213>  Macaca fascicularis

<400>  36

Met Ala Phe Leu Val Ala Pro Pro Met Gln Phe Val Tyr Leu Leu Lys 
1               5                   10                  15      


Thr Leu Cys Val Phe Asn Met Val Phe Ala Lys Pro Gly Phe Ser Glu 
            20                  25                  30          


Thr Val Phe Ser His Arg Leu Ser Phe Thr Val Leu Ser Ala Val Gly 
        35                  40                  45              


Tyr Phe Arg Trp Gln Lys Arg Pro His Leu Leu Pro Val Ser Pro Leu 
    50                  55                  60                  


Gly Arg Ser Met Arg Trp Cys Leu Phe Leu Ile Trp Ala Gln Gly Leu 
65                  70                  75                  80  


Arg Gln Ala Pro Leu Ala Ser Gly Met Met Thr Gly Thr Ile Glu Thr 
                85                  90                  95      


Thr Gly Asn Ile Ser Ala Lys Lys Gly Gly Ser Val Ile Leu Gln Cys 
            100                 105                 110         


His Leu Ser Ser Thr Met Ala Gln Val Thr Gln Val Asn Trp Glu Gln 
        115                 120                 125             


His Asp His Ser Leu Leu Ala Ile Arg Asn Ala Glu Leu Gly Trp His 
    130                 135                 140                 


Ile Tyr Pro Ala Phe Lys Asp Arg Val Ala Pro Gly Pro Gly Leu Gly 
145                 150                 155                 160 


Leu Thr Leu Gln Ser Leu Thr Met Asn Asp Thr Gly Glu Tyr Phe Cys 
                165                 170                 175     


Thr Tyr His Thr Tyr Pro Asp Gly Thr Tyr Arg Gly Arg Ile Phe Leu 
            180                 185                 190         


Glu Val Leu Glu Ser Ser Val Ala Glu His Ser Ala Arg Phe Gln Ile 
        195                 200                 205             


Pro Leu Leu Gly Ala Met Ala Met Met Leu Val Val Ile Cys Ile Ala 
    210                 215                 220                 


Val Ile Val Val Val Val Leu Ala Arg Lys Lys Lys Ser Leu Arg Ile 
225                 230                 235                 240 


His Ser Val Glu Ser Gly Leu Gln Arg Lys Ser Thr Gly Gln Glu Glu 
                245                 250                 255     


Gln Ile Pro Ser Ala Pro Ser Pro Pro Gly Ser Cys Val Gln Ala Glu 
            260                 265                 270         


Ala Ala Pro Ala Gly Leu Cys Gly Glu Gln Gln Gly Asp Asp Cys Ala 
        275                 280                 285             


Glu Leu His Asp Tyr Phe Asn Val Leu Ser Tyr Arg Ser Leu Gly Ser 
    290                 295                 300                 


Cys Ser Phe Phe Thr Glu Thr Gly 
305                 310         


<210>  37
<211>  241
<212>  PRT
<213>  Mus musculus

<400>  37

Met His Gly Trp Leu Leu Leu Val Trp Val Gln Gly Leu Ile Gln Ala 
1               5                   10                  15      


Ala Phe Leu Ala Thr Gly Ala Thr Ala Gly Thr Ile Asp Thr Lys Arg 
            20                  25                  30          


Asn Ile Ser Ala Glu Glu Gly Gly Ser Val Ile Leu Gln Cys His Phe 
        35                  40                  45              


Ser Ser Asp Thr Ala Glu Val Thr Gln Val Asp Trp Lys Gln Gln Asp 
    50                  55                  60                  


Gln Leu Leu Ala Ile Tyr Ser Val Asp Leu Gly Trp His Val Ala Ser 
65                  70                  75                  80  


Val Phe Ser Asp Arg Val Val Pro Gly Pro Ser Leu Gly Leu Thr Phe 
                85                  90                  95      


Gln Ser Leu Thr Met Asn Asp Thr Gly Glu Tyr Phe Cys Thr Tyr His 
            100                 105                 110         


Thr Tyr Pro Gly Gly Ile Tyr Lys Gly Arg Ile Phe Leu Lys Val Gln 
        115                 120                 125             


Glu Ser Ser Val Ala Gln Phe Gln Thr Ala Pro Leu Gly Gly Thr Met 
    130                 135                 140                 


Ala Ala Val Leu Gly Leu Ile Cys Leu Met Val Thr Gly Val Thr Val 
145                 150                 155                 160 


Leu Ala Arg Lys Lys Ser Ile Arg Met His Ser Ile Glu Ser Gly Leu 
                165                 170                 175     


Gly Arg Thr Glu Ala Glu Pro Gln Glu Trp Asn Leu Arg Ser Leu Ser 
            180                 185                 190         


Ser Pro Gly Ser Pro Val Gln Thr Gln Thr Ala Pro Ala Gly Pro Cys 
        195                 200                 205             


Gly Glu Gln Ala Glu Asp Asp Tyr Ala Asp Pro Gln Glu Tyr Phe Asn 
    210                 215                 220                 


Val Leu Ser Tyr Arg Ser Leu Glu Ser Phe Ile Ala Val Ser Lys Thr 
225                 230                 235                 240 


Gly 
    


<210>  38
<211>  417
<212>  PRT
<213>  Homo sapiens

<400>  38

Met Ala Arg Ala Met Ala Ala Ala Trp Pro Leu Leu Leu Val Ala Leu 
1               5                   10                  15      


Leu Val Leu Ser Trp Pro Pro Pro Gly Thr Gly Asp Val Val Val Gln 
            20                  25                  30          


Ala Pro Thr Gln Val Pro Gly Phe Leu Gly Asp Ser Val Thr Leu Pro 
        35                  40                  45              


Cys Tyr Leu Gln Val Pro Asn Met Glu Val Thr His Val Ser Gln Leu 
    50                  55                  60                  


Thr Trp Ala Arg His Gly Glu Ser Gly Ser Met Ala Val Phe His Gln 
65                  70                  75                  80  


Thr Gln Gly Pro Ser Tyr Ser Glu Ser Lys Arg Leu Glu Phe Val Ala 
                85                  90                  95      


Ala Arg Leu Gly Ala Glu Leu Arg Asn Ala Ser Leu Arg Met Phe Gly 
            100                 105                 110         


Leu Arg Val Glu Asp Glu Gly Asn Tyr Thr Cys Leu Phe Val Thr Phe 
        115                 120                 125             


Pro Gln Gly Ser Arg Ser Val Asp Ile Trp Leu Arg Val Leu Ala Lys 
    130                 135                 140                 


Pro Gln Asn Thr Ala Glu Val Gln Lys Val Gln Leu Thr Gly Glu Pro 
145                 150                 155                 160 


Val Pro Met Ala Arg Cys Val Ser Thr Gly Gly Arg Pro Pro Ala Gln 
                165                 170                 175     


Ile Thr Trp His Ser Asp Leu Gly Gly Met Pro Asn Thr Ser Gln Val 
            180                 185                 190         


Pro Gly Phe Leu Ser Gly Thr Val Thr Val Thr Ser Leu Trp Ile Leu 
        195                 200                 205             


Val Pro Ser Ser Gln Val Asp Gly Lys Asn Val Thr Cys Lys Val Glu 
    210                 215                 220                 


His Glu Ser Phe Glu Lys Pro Gln Leu Leu Thr Val Asn Leu Thr Val 
225                 230                 235                 240 


Tyr Tyr Pro Pro Glu Val Ser Ile Ser Gly Tyr Asp Asn Asn Trp Tyr 
                245                 250                 255     


Leu Gly Gln Asn Glu Ala Thr Leu Thr Cys Asp Ala Arg Ser Asn Pro 
            260                 265                 270         


Glu Pro Thr Gly Tyr Asn Trp Ser Thr Thr Met Gly Pro Leu Pro Pro 
        275                 280                 285             


Phe Ala Val Ala Gln Gly Ala Gln Leu Leu Ile Arg Pro Val Asp Lys 
    290                 295                 300                 


Pro Ile Asn Thr Thr Leu Ile Cys Asn Val Thr Asn Ala Leu Gly Ala 
305                 310                 315                 320 


Arg Gln Ala Glu Leu Thr Val Gln Val Lys Glu Gly Pro Pro Ser Glu 
                325                 330                 335     


His Ser Gly Ile Ser Arg Asn Ala Ile Ile Phe Leu Val Leu Gly Ile 
            340                 345                 350         


Leu Val Phe Leu Ile Leu Leu Gly Ile Gly Ile Tyr Phe Tyr Trp Ser 
        355                 360                 365             


Lys Cys Ser Arg Glu Val Leu Trp His Cys His Leu Cys Pro Ser Ser 
    370                 375                 380                 


Thr Glu His Ala Ser Ala Ser Ala Asn Gly His Val Ser Tyr Ser Ala 
385                 390                 395                 400 


Val Ser Arg Glu Asn Ser Ser Ser Gln Asp Pro Gln Thr Glu Gly Thr 
                405                 410                 415     


Arg 
    


