                         SEQUENCE LISTING

<110>  Biocytogen PharmaceuticalsBeijingCo., Ltd.
 
<120>  GENETICALLY MODIFIED NON-HUMAN ANIMAL WITH HUMAN OR CHIMERIC 
       VSIG4

<130>  1

<150>  CN202110615989.7
<151>  2021-06-02

<150>  CN202111470150.5
<151>  2021-12-03

<160>  60    

<170>  PatentIn version 3.5

<210>  1
<211>  280
<212>  PRT
<213>  Mus musculus

<400>  1

Met Glu Ile Ser Ser Gly Leu Leu Phe Leu Gly His Leu Ile Val Leu 
1               5                   10                  15      


Thr Tyr Gly His Pro Thr Leu Lys Thr Pro Glu Ser Val Thr Gly Thr 
            20                  25                  30          


Trp Lys Gly Asp Val Lys Ile Gln Cys Ile Tyr Asp Pro Leu Arg Gly 
        35                  40                  45              


Tyr Arg Gln Val Leu Val Lys Trp Leu Val Arg His Gly Ser Asp Ser 
    50                  55                  60                  


Val Thr Ile Phe Leu Arg Asp Ser Thr Gly Asp His Ile Gln Gln Ala 
65                  70                  75                  80  


Lys Tyr Arg Gly Arg Leu Lys Val Ser His Lys Val Pro Gly Asp Val 
                85                  90                  95      


Ser Leu Gln Ile Asn Thr Leu Gln Met Asp Asp Arg Asn His Tyr Thr 
            100                 105                 110         


Cys Glu Val Thr Trp Gln Thr Pro Asp Gly Asn Gln Val Ile Arg Asp 
        115                 120                 125             


Lys Ile Ile Glu Leu Arg Val Arg Lys Tyr Asn Pro Pro Arg Ile Asn 
    130                 135                 140                 


Thr Glu Ala Pro Thr Thr Leu His Ser Ser Leu Glu Ala Thr Thr Ile 
145                 150                 155                 160 


Met Ser Ser Thr Ser Asp Leu Thr Thr Asn Gly Thr Gly Lys Leu Glu 
                165                 170                 175     


Glu Thr Ile Ala Gly Ser Gly Arg Asn Leu Pro Ile Phe Ala Ile Ile 
            180                 185                 190         


Phe Ile Ile Ser Leu Cys Cys Ile Val Ala Val Thr Ile Pro Tyr Ile 
        195                 200                 205             


Leu Phe Arg Cys Arg Thr Phe Gln Gln Glu Tyr Val Tyr Gly Val Ser 
    210                 215                 220                 


Arg Val Phe Ala Arg Lys Thr Ser Asn Ser Glu Glu Thr Thr Arg Val 
225                 230                 235                 240 


Thr Thr Ile Ala Thr Asp Glu Pro Asp Ser Gln Ala Leu Ile Ser Asp 
                245                 250                 255     


Tyr Ser Asp Asp Pro Cys Leu Ser Gln Glu Tyr Gln Ile Thr Ile Arg 
            260                 265                 270         


Ser Thr Met Ser Ile Pro Ala Cys 
        275                 280 


<210>  2
<211>  399
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Gly Ile Leu Leu Gly Leu Leu Leu Leu Gly His Leu Thr Val Asp 
1               5                   10                  15      


Thr Tyr Gly Arg Pro Ile Leu Glu Val Pro Glu Ser Val Thr Gly Pro 
            20                  25                  30          


Trp Lys Gly Asp Val Asn Leu Pro Cys Thr Tyr Asp Pro Leu Gln Gly 
        35                  40                  45              


Tyr Thr Gln Val Leu Val Lys Trp Leu Val Gln Arg Gly Ser Asp Pro 
    50                  55                  60                  


Val Thr Ile Phe Leu Arg Asp Ser Ser Gly Asp His Ile Gln Gln Ala 
65                  70                  75                  80  


Lys Tyr Gln Gly Arg Leu His Val Ser His Lys Val Pro Gly Asp Val 
                85                  90                  95      


Ser Leu Gln Leu Ser Thr Leu Glu Met Asp Asp Arg Ser His Tyr Thr 
            100                 105                 110         


Cys Glu Val Thr Trp Gln Thr Pro Asp Gly Asn Gln Val Val Arg Asp 
        115                 120                 125             


Lys Ile Thr Glu Leu Arg Val Gln Lys Leu Ser Val Ser Lys Pro Thr 
    130                 135                 140                 


Val Thr Thr Gly Ser Gly Tyr Gly Phe Thr Val Pro Gln Gly Met Arg 
145                 150                 155                 160 


Ile Ser Leu Gln Cys Gln Ala Arg Gly Ser Pro Pro Ile Ser Tyr Ile 
                165                 170                 175     


Trp Tyr Lys Gln Gln Thr Asn Asn Gln Glu Pro Ile Lys Val Ala Thr 
            180                 185                 190         


Leu Ser Thr Leu Leu Phe Lys Pro Ala Val Ile Ala Asp Ser Gly Ser 
        195                 200                 205             


Tyr Phe Cys Thr Ala Lys Gly Gln Val Gly Ser Glu Gln His Ser Asp 
    210                 215                 220                 


Ile Val Lys Phe Val Val Lys Asp Ser Ser Lys Leu Leu Lys Thr Lys 
225                 230                 235                 240 


Thr Glu Ala Pro Thr Thr Met Thr Tyr Pro Leu Lys Ala Thr Ser Thr 
                245                 250                 255     


Val Lys Gln Ser Trp Asp Trp Thr Thr Asp Met Asp Gly Tyr Leu Gly 
            260                 265                 270         


Glu Thr Ser Ala Gly Pro Gly Lys Ser Leu Pro Val Phe Ala Ile Ile 
        275                 280                 285             


Leu Ile Ile Ser Leu Cys Cys Met Val Val Phe Thr Met Ala Tyr Ile 
    290                 295                 300                 


Met Leu Cys Arg Lys Thr Ser Gln Gln Glu His Val Tyr Glu Ala Ala 
305                 310                 315                 320 


Arg Ala His Ala Arg Glu Ala Asn Asp Ser Gly Glu Thr Met Arg Val 
                325                 330                 335     


Ala Ile Phe Ala Ser Gly Cys Ser Ser Asp Glu Pro Thr Ser Gln Asn 
            340                 345                 350         


Leu Gly Asn Asn Tyr Ser Asp Glu Pro Cys Ile Gly Gln Glu Tyr Gln 
        355                 360                 365             


Ile Ile Ala Gln Ile Asn Gly Asn Tyr Ala Arg Leu Leu Asp Thr Val 
    370                 375                 380                 


Pro Leu Asp Tyr Glu Phe Leu Ala Thr Glu Gly Lys Ser Val Cys 
385                 390                 395                 


<210>  3
<211>  4306
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' homologous arm

<400>  3
gccacagtga tccctgctta tgaggcttga aatttttgca tcattttctc tgctgatcat       60

ccataatatg taagttcttt caacacatgc accattatcc ctggaagcac tagaattgaa      120

catgaaaagg taaattctct ggattaaagt aactacattc cttccttctc ccactctaat      180

actgtttcct ttctgagcca gctcaagtac tagcctgcta tactgaagaa aaagggcaca      240

acccttgaga tcaagccata cctgagtgca aatttcccct ttgctcatta ttttttgact      300

gtgtataggt gctatagtga gtctttatat aaatttcctc taacaacaaa gattatacaa      360

gaactagtgt atatctcagc atatcaatgc atactgagca agtacaaggc cctagttgaa      420

atgacctgca ccacaaataa aaagactcac aatgtctttc ttaaaataca aagcaagcat      480

aacagtgagg tacttagcag gatcattttg gagatatgca gatattgagt ataaatatta      540

tctaggtatt tctgtactaa tgatttcaca aattctagtc ttttggttca tttttagaat      600

agaaatgtat ttaattcagt caactcaaca aagtatttat taattattat attatatgtc      660

tggttgtctt tagtgaacat gactatataa aagtaatgcc ttgtttatac ctacattaca      720

gaatgtacag tgaaaaatta aagagatagt aaatgtcagg tacaagcact atagagacaa      780

aagaagaaat gtaagagaat agagatgaaa catataaaga tacaagagta agttcactaa      840

tactcatttt agtacagtgt ctgaatgaat aaaagatgga aaatgccagt tgttattgct      900

gtagccgata ttgtatagat aatgtggctt gatcattttt aatttacaac acacaattac      960

caagtctcat catgttcatg ttacaactga gaaagctaat attcagaagg gttttatttt     1020

tctaaggcct agaattaaaa ctttcaatct aataaagcat tcccttatta agtctaacaa     1080

actacaaata aggcttatct ttctctttaa aaaaataaaa tatagatgtc tttacaaagg     1140

catcatgagg aatactaatt aaagctagtc gtttcttgac atcagagatg gggctactgg     1200

caaaagtcag tcaacaaact ttcccactgt gagatatgcg acaccctcca aagcaactat     1260

atccctagtc ttaacaacta ataaaacaat ttctgtgtag aaagtcatcc agtgcactaa     1320

actcatacta tgaagcactc gagtgcctct aattcacaca gtgtccattt gtattgagga     1380

ctatggagct aatggtacaa attatgaaaa ctgcatatgc cagaacaaat ggtttcccta     1440

gactgaccag aaagtaaaac atccctctat ggtaaacttt gagcagactc aaaattctgg     1500

gacattaaag accagagagc accactggtc ccagagaaag tcaaatccta gtagaatgag     1560

gaacagtttc acccatcctt aaatctctaa ccttacgcag cgtaaaaaga aagaaccctg     1620

tgtgactctt ctgaaatacg gaaggtgggg gaagtgtgtg aggaaactag gattctgagg     1680

gaataaaata gaaaagcaac acagaaactg gcagctacca gcacttccag gttcttcagc     1740

agcaagagga tggaaggatg aatagaagta gcttcaaata ggatggagat ctcatcaggc     1800

ttgctgttcc tgggccacct aatagtgctc acctatggta aggcaggata ttgatacttg     1860

actacctctt caccctctgg cagaaaatgt gagtgttgac tttgagtgtt taccgagaaa     1920

aacttgtttc tcctgggcag tcacagttgc ctgcaattct acaggctttc tgaatgttga     1980

gcagaaggga agaaagtctg gtaaagcttc cagaaactgc cttggataga aattgaaaga     2040

gttcttctct tagaaaatat ctgcatcact ttccaatatt tcagggatgt cagaggactc     2100

caatgtcatg caaactttcc atattctcct tttcccttga aattttataa ttatgactaa     2160

ctttccattt ccaagctttg attcttgctg cagtatatac ctccacaatg aataaatgtt     2220

gttgagtaaa tagatgtgac cttagaagta acctatagcc aaggatgttg aggtacacct     2280

ttaatcctag ctgttgggag gtagaggcac tctgatctct gtgatctcaa ggacactttc     2340

gactacatag aaagttccag gatatctaag gttacatggt gagactcagt ttgtaaataa     2400

atagataaaa gtaacacatg ccactactta gaattgttta caagtgctca gcacttgcta     2460

ttcatacact tttagaacac agacatttac tgatatccat gctactctaa gacttctcac     2520

gatcaaccca gaacagatac tattcctttt ttttcatgtc agcacttcaa acatctaaag     2580

aacggaaaga actgtatcct cttaattttc ttctctaaac taaatatctc tagcttcttt     2640

aactaaagga tgtggtatac tactagctgc tgggataact cacttccctg tgggcatagt     2700

agcccagttg tagggcctgt ttgcagggat ctaattctag attcatagtc cagcagagtc     2760

tgggatatct catagtagca ttatttctgt gactcttctt ccactctcac tattcaagaa     2820

aatgaaatgc cttttcagga aactaagtac aagtaataac tacttaagac ttggtatagg     2880

gtaaaaggat ttttttgctg aatttctgct tgcttcatct tcctataggg tgtcattggc     2940

ttaaacataa tttgaagtac tcagcaatga tgggctatgt ttatgctttt taattttatt     3000

ttattgaagt tgacccctct aatggttaaa atatgccgtg tattttccaa gagttcctgg     3060

tgaggcaaag accatagatg ccacttcagt gttcagatac attagtatat gctttggtac     3120

ttagcagtgt atcctgaaag tcagataaca taacctcgct ccatgcacaa ggcatcgtag     3180

tctacctgct taactgaaaa tatgctaaat aaaaaagatt cagtcagtga taccttgcat     3240

tttgaaacta tatctaccaa gaaagacatt gccaaaaaac agcccctgga cccagttgaa     3300

taaaagtcat caagatatga gcatacatat gaaaacctct aatacaatgg gcacccatct     3360

ctttttgcct ctggactccc tccaactccc cagcccatca agaagcaatt ttctttgttt     3420

tcaccacaca atagctagca caaccagtgc tagagagtta ctcaggttct tttcttttcc     3480

agaccaagta tggatgaaat cttttccccc tcaggatatc attttctaat atctttttgg     3540

taagaactaa aggaaatgaa aaaaaaaaaa aaaacaaaga gaaaagagaa ataaggacag     3600

aaattggcta gacttgcttg caaagtacat gtttcaaatc taatagctaa tggtttggtt     3660

aaataggaat atactccaga atttttgctt caccctatgt ttcatcttta gagtcttcta     3720

tagctcatct ctttcaccct gccagaaaag aatttgattt tatgtcccaa tcactgagcc     3780

tttctcttga atgagtctgt ttcaagcagc aaactgtaca aaatgaagta ctttggtttc     3840

cactattcac cattttcctt cctacatatc atttgaaaat agtgaaagaa aatattatat     3900

atatgtacat atatttacat attctataat attttacata tatgcactta tatgtgtgtg     3960

catacatata catgtaaaat attatagaat tctaaaaatt attctatcct aactctcaca     4020

taatatttgg aggaaaatgg atccttcaca gtggtagtga cttaccgagg gtcacttact     4080

ggcaagacag tgtcgtgaaa ggagcttaaa agcactagag ctaataagtg ttaaagtctt     4140

tgtcgtaggg actgactcac attgatggga aagttaacag accagaagag cacagggatg     4200

aaggaagggt gtttcccaga aacctattga gtctgtcact cataccctca ctccagtgaa     4260

cattttgtct gtgtttgcct ttttgacttt gttccctgtt ttaggc                    4306


<210>  4
<211>  4339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' homologous arm

<400>  4
tgaaagttcc ctataccctc cctccgccct gctcccctac ccacccacgc ccacttcttg       60

gccctggcat tcccctgtac tggggcatat aaagcttgca agaccaaggg gcctctcatc      120

caactaggcc atcctctgct acacatgtag ctattgttgt tgtgtatatt gttgttccac      180

ctatagggtt gcagacccct tcagctcctt gggtgctttc tctagcttct ccattgggga      240

ccctatgttc catctttttt tttccatttt tattaggtat ttagctcatt tacatttcca      300

atgctatacc aaaagtcccc catacccacc cacccccact cccctaccca cccactcccc      360

ctttttggcc ctggcgttcc cctgtactgg ggcatataaa gtttgcgtgt ccaatgggcc      420

tcactttccc tatgttccat cttatagatg actgtgagca tccacttcat tttacagaaa      480

atttaaccca gggttaacca acaattttcc cactgaacta aaatattgta ctatttttac      540

ttcttatctt tttttttgtg gtactttttt tctttttttt tctttttttt tattacgtat      600

tttcttcaat tacatttcca atgctatccc aaaagcccca caccacactc ccctacccac      660

ccattcccat tttttggccc tggcattccc ctgtactggg gcatataacg tttgcctgac      720

caatgggcct ctctttccag tgatggccga ctaggtcacc ttttgataca tatgcagcta      780

gagtcaagag ctctggggta ctggttagtt cataatgttg ttcctccaga tctctttagc      840

tccttggata ctttctctag ctcctccatt ggggaccctg tgatccatcc aatagttgac      900

tgtgagcatc ctcttctgtg tttgctaggc cccggcctag tctcacaagg gacagctata      960

tcaccgtcct tgcaacaaag gcttgctagt gtatgcaatg gtgtcatcgc ttggaggcta     1020

attatgggat ggatccctcg atatggcagt ctctagatgg accatccttt tgtctcagct     1080

ccaaactttg tctctgtaac tccttccatg ggtgattgtt tccaattcta agaaggagca     1140

aagtgtccac actttggtct ttgttcttct tcagtttcat atgttttaca tattgtacct     1200

tatatctcgc tatactattt acttcttatc ttgatgatat catactaacc tagccaggct     1260

tgcctttatt tcactctcta aattaggtag gctttgaact tgccacctgc cttagtctcc     1320

caagtaattg gaatgatagg cctgtaactc taatccaagc tttggttttt gttgtctgtt     1380

tgtttcttgg tttgtctttc accttccaac tcatgccacc tcccttctca taatatggat     1440

agccctatct aaagcaaatc aatttttgct taaatagtat tctacataac atccactacc     1500

cagtccatct ttctatcttc tctactttgt gactcttctc ggggcaacag gaagattgtc     1560

aagaagcctc acatctttat caacgtacta tgaatgtaca aataaagtag tttctgtctt     1620

ttcaaagtct gtgtataata tcactaaaat atctggacta gttgcattgg tcatcttttc     1680

tcctcaattc tttttataat gtgaagagac ttttttaaaa ttttattaat ataggttggt     1740

ttatttgaga aagtggtaag atgggtttca atgtttttta aagctttatg catataaatg     1800

ggaagagtaa gagattattt cttaattgat ggaacggggt ggggggaatg aagaaaagtg     1860

tagggaacag gagggtaggg gaaaggaaag aaggaactct atgtactttg gaatgaaact     1920

cccttgtaaa gaaattgttt ggggccacgt ttgcattgct tcctttcccc ccatttattt     1980

catgtcgaat caacttccta aattttcatg ggaactacca tgttgcctat gtagtctgca     2040

tatgcacagg atcagatcag gtttcaacaa gaggtactcc cactttgaaa tgactgcaaa     2100

agtcaaaata gtacacaaaa gtgattgaca gtctgcccta ttttaccccc actgatgctg     2160

ccaagtctat gaccaaaatc tcagcatttt cttctttgac aaatgctagc tatgcataat     2220

tagtgtcaga gatgacttct tcttctccat ctcacttacc agcctccaca acctgggtac     2280

cacccagtag aaacctcagc ttccttcatg aaacatctgt gagtccagcc ctgaaagcct     2340

tgaaaacacc ctttatctca ttctgctctt tagaattaat ccctgggctt gaagagagaa     2400

tgtttattga aattgcccta gtaaagtgag ccacaaattc agggaacccc caacactgga     2460

accaccagga atgtctccta ttcttgcatg tgaaaatgtt tacagattac ttatctgaac     2520

attcacatac cacaaattgg gaagtgaagc ccagagagaa atgaaggttt gtcaaaagta     2580

acatacatca agtcaatggc agaacaaaga ctggaggtta gacctgtgga tttttagcag     2640

tgtgtgtact ttgctgcaga catcttctga agatggtctt tgtccctaaa caatggtatt     2700

tttccatgca gggtaagcca gtaagtcatc cctagataga gatgcatccc tccgacaatt     2760

ctgagcaaaa ttggggtaac aatatgggtc ttgttattta ggtcactggt tagtgggtga     2820

aaagttaaag aatgaaagta ggtatctcca gaaccaaggg agagtatgga atttctccag     2880

aaccaaggga gagtatggaa tttctctaaa gaagcttgcc aaaatatatg ttatgaaatg     2940

ttagactctg gagagaggga ttggagatgc ttcattttat agttctatat tagaagttca     3000

ccccagtgca atgatagaat attaaaggct cagaaaattc tgtttaaaaa gacacgtgta     3060

aaccaataat tcaaaaaatt tttccctctg agtctattct ttcttcttgc cctcctgccc     3120

cagacctaaa tcttatttct cagaggaaaa aaaaatccat tttggttacc atcttgtata     3180

cactatagca ttagaatacc cacagtttga tttatttttt tcgcaaaacc ctggttggta     3240

tttgacctat atagcaaaag gttattgact agtaaaacat caattaattg gtaaatattt     3300

attaataatt aaaatgttgg acaatggtat gagagacagc aatcaactga atataataag     3360

ctctagattc ttacaatata atagggaatt caagaacata ccctggaaaa attcactgac     3420

aactttagaa gatgtgttgg atggtaggac atataatagc acataccagt atgtggttag     3480

ccaaggagta aagaaaagta taggttaact ttcaatagtg ccaagaggaa ttggaatttg     3540

gtagtgtctg aaagcccaat gaagataaac aagaaggttc ttttaagaat gataaaagca     3600

aagtggaaaa gagaaaatgt aggtagctta agatgtgctc cgaaggccag acagtggata     3660

tttatttcct actttctcat agaattcatg atgcttgtat gaaaattctg ctattctggc     3720

tgcaagaaga ggaaagtgaa agaagacatt aataataaat caaataatac tctcattccc     3780

tgtatccctc atgtaccact atgcagtcca tggcattttc acaatgtaca cagatgcata     3840

ccagacttgt agaccccaga gctgcatcgt tcctactctg atgttctctg aataaatgtt     3900

atagttccct taaagacata gaataacacc agagagcttc atgtttcata agcattcata     3960

tgtaggcagc aggctatgtg ctctgtcctg gacctggagc tgataggcat gagccacact     4020

tggggtattt gacaaactga cactctacca cacagagtgt ccaactcagc ttgtcaaata     4080

cacggagcgt gacactgcag atggccagat caggaacctc cactgggcag caagaacatg     4140

ctggaagatg tgcaactgga aagagaaaaa aatagggtga ttatatctta ccctaagtgt     4200

gagacaaatg gcagacacta acatgactga ctaagacaca ccaaaagagg gaagtgaaca     4260

aaacacgcta atgctccaaa caaaaacggg gatcctgggt gtttgtctgg agattctggt     4320

ggcccaagcc tcaattaac                                                  4339


<210>  5
<211>  8707
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of 66025122-66033828 of NCBI accession number
       NC_000023.11

<400>  5
cgtcccatcc tggaagtgcc agagagtgta acaggacctt ggaaagggga tgtgaatctt       60

ccctgcacct atgaccccct gcaaggctac acccaagtct tggtgaagtg gctggtacaa      120

cgtggctcag accctgtcac catctttcta cgtgactctt ctggagacca tatccagcag      180

gcaaagtacc agggccgcct gcatgtgagc cacaaggttc caggagatgt atccctccaa      240

ttgagcaccc tggagatgga tgaccggagc cactacacgt gtgaagtcac ctggcagact      300

cctgatggca accaagtcgt gagagataag attactgagc tccgtgtcca gaaacgtgag      360

tcactatggg ggtaggaaag cactgataat caatgaatca atagtggatg taattctaga      420

aaagcctgaa aaatccagag gccttcgttt atacagaggc tctccacagg tatatgtgct      480

tatcagagga agtggacagg cagaggattc ttagggtcag cattcccata gttaaattta      540

ggaagtgctg ggaagggttc ctgacctgca aatctctatc ccttcgagtg tcccagaaat      600

gccagtatcc atggactagt taccctcctt gggctctggg ctttacaact caaaaatgag      660

tacatttaat ttgtagatag atgctgatag agaagctctg gggaaaggga ggcagaggtt      720

agccaggata agtttgcttg ctggacaatt ttacaaaagt ctggccctga taatccacaa      780

ctgcaggtaa tcaaaagctc tacttgagcc ccagaacaaa ctacattaat tttaaaggtc      840

agttgatttt tacttccaag tattccaaaa ccccgggtaa taccaaaaag tacctacctt      900

caaaggaagc cattttattt gtattgccct ttccttctta tttcctccaa agtcagaagc      960

cccctttagt tccagttgca gatatatgca tgcccttacg cccaacaaga atgattcagc     1020

cttttggtcc catatcctta tgactgccca gagtttcctg tcttgtcttt tctttgcagt     1080

ctctgtctcc aagcccacag tgacaactgg cagcggttat ggcttcacgg tgccccaggg     1140

aatgaggatt agccttcaat gccaggctcg gggttctcct cccatcagtt atatttggta     1200

taagcaacag actaataacc aggaacccat caaagtagca accctaagta ccttactctt     1260

caagcctgcg gtgatagccg actcaggctc ctatttctgc actgccaagg gccaggttgg     1320

ctctgagcag cacagcgaca ttgtgaagtt tgtggtcaaa ggtgagcggc cctctttcct     1380

ggtgagcatc ttaacacaca agcttgaaac aaagatacct caaaaggtgc ccttatgcaa     1440

aagaagaatt gggagaggag gatcccaagc aggcaatggt tgtacaatca caaagaaaaa     1500

aaacttggac tggggacaac agggatagag acattgccta tcatgccata ggttaagtag     1560

ataaagacgg taaaggaaag agagatttac tagcacattg tatttgccag atacaaagta     1620

caataacttg acacacattt tctcattgaa tccccacaac tgtcccatga gatagttatc     1680

attactctta ctttacagaa gaggaaacag agtcagaaag gggaatctac ttgcccatgg     1740

tctcacagca aggaagtata aatctggatt tgtactcaga tttcatgctc ttcctactat     1800

gtctgattgt ttctcatggt tgaatctata tcagatcctg ggcacccttt gaaagctgtt     1860

gttttttgtt tgtgtgttct gtttgtttgg ttattgtttt gcttgtttga ttaggcttga     1920

tctaatatat ctacatgctt tcttttaact tccctccccc acttcattgt aaacccaaat     1980

acaagttctg gttgaagtat cctttaagat gggctcttgt taatcaagat ctgactattt     2040

cttacagcac tgtcctggga gcactacaaa ctatgcatat aaagactgta agctgaggct     2100

taaatgaaga gtccagcagt gatatgtcca gatgagtttt cttagctttc aagagatctc     2160

cttggaaaga aagacacccc aacaaggtta ccattgccca aaacatgttg gaaactaact     2220

gattttaggg aattgccctc agaaccatct gaaaattatt gtcttctgag gatagttctg     2280

tgtaaggaga gggcgtaaac ctagcccaaa tggcttatta ccatttgggc taggagactg     2340

atgttggttt gagactcttg tgtttgcctg gaggaatttg tttcccaaag ttaagctgta     2400

gtgaggatgt gtgtgctgta gatcatggtt tgttagtttt gggaggaaca agagtggtcc     2460

gccctgaaag ttatatgaac agtgtcccag ttctctaaga gctgacagtc tggtcaagca     2520

gagagggcag ggcgtgacca gaaagcccaa gaatgtatac ttcataaaga gactaattga     2580

acattcttgc ctttctgtga gttacttctc agaccaccta ggcaagaact tgctcacagt     2640

aaaccctcct gtttattcct tctcacagtc aatgcttatt acagtgctgt taagcagtgc     2700

tcgccattgc cccctactga cagataggaa aactgaggct cagggaagtt attctcagcc     2760

ttccactctt ccctgctctg tgagccaacg taatctttcc tggctacctc tacctgcctc     2820

cctgtccacc caaccttgtc ccctgagtct gaccccctat ttcacctctg ccctacggaa     2880

ctctcagccc tttcaccctt ccacctcact ctgcactgtt ctttagttct agaatgactc     2940

tggaggccct gcttttgctc atgttcttgg actgctgagc tttgctgacg aacaaaacaa     3000

aacacagata tgaattgact ccacttgaat tttatgctct gcgatatgta ctgcctgacc     3060

ccatgccgtg tgtctatttg cttatctttg tctcattcct catagcagct tttcagcaca     3120

tttctacctt atttcaggcc cccagccttg ctcctaaaag ataaggtaac ttccttcctc     3180

tgtaagaagg tccagaccct cttcatatgt gaattttatc aactaccctc ctctctgcct     3240

tgatatttcc ctatccattt cctcttggct cagagtaagg tatgctttgc tcctctccac     3300

aacaagcccc tcctcctgaa ctcttaacat cagctactcc aatgccctgc aatacccttt     3360

atcctctacc taagccaaat agatatgcat gtataatata tgtatatatg tcagtatgca     3420

agcctaatac atacatatat atatgttatt tttcaaaaat ctttctctcc atctgccacc     3480

tcatctctcc tatgttcact accaaccttc tcacgaaggt agcttccacc agctgaacct     3540

ctcaattcat tcaccacaac ataactttgt aatttggggt catcattaac ctccatatca     3600

ccaaatccca agacctctcc attcatactt ttcctcctca acatctgtgt agcagtggat     3660

cctgttggcc atttcttcca cctcaatatt cttttaatca ctgctattga tgacaccaac     3720

ttatcttggt tcttctaaga ccttcctgaa tgatccttct gagacacctt tgttggctct     3780

tttttcattc actccttaat tgtaaatgtt ctcaaagact caattcttat tcatctctgc     3840

atgtatttct atattatcag gagattttat ttacttctgt ggcttcaaat atcacctgta     3900

gtctgatgac tctccaacca taatattatc tgtatttatt ttttcctgac tgctagatcc     3960

cagtgtctag ttaccttctg gacagcttca tttctaattt cagcgtgttc aaaactgatc     4020

gtacctttta ctcaccaaac ctgttcctca aacctgttag aaaacatagc agtacctttg     4080

actcctcctt ctcttttacc tcgcatcgtc aaataatcat caatattacc aagtgctgtg     4140

aattcaccta ctgaatcaga taccttatta ggctctttct ttctcttcct ataccctcat     4200

ggtctcatat tcttttttaa aaaacaattt caatcatctt ctaaatgttc tccccatgtt     4260

catacattct tacgaagtca tcaacttatt caatatttat tgagtatata ttttatgtca     4320

ctagattata aaattcaaga gtgcagaatt ctacctgtct acttcattgc tgtttttcag     4380

agcccagcaa agtatctggc acaaagaaga cactagacac tagatgaata ctgtatttgt     4440

taatgaatgg ggaaaatatg ctattgcctt acctttgaaa atcctaccac actgttgtca     4500

gaattatttc caaaaaaaat agactgaaca atttttgctt cctttctcaa aaggaagtaa     4560

acctttctca aaaggtgtaa ccttctgtta caccccatgg atctttccaa ctaaagaaca     4620

aagcccaaac ccttttagtc tgttttcagc ctacctatcc agacttatct actaatatgt     4680

ttcgccacat ctaaaacgga gcatgctgtt tcctctgcta gcaagaggga gaagcttatg     4740

gttttctcat gtcttttctg agcaggtaca tggccctgtg cttgtgtgtg gcctcctagt     4800

ttcccagaac tgtgtcagaa ctcttcaaaa tccctatgga aatcttattc ctcaaccatt     4860

tttaaggcta ttttggttag ttttttttgt tttctcgcct attatccatt gcctcaggca     4920

gctgtgtgac aaatgctacc ccatccctgg ggagaggctc ttagcactgg gtgaccaata     4980

aaacaagtct tttgagtcag gtctttcagg gaaccaccag acaggccaaa taatggcaat     5040

tattcaaaaa tgaagtttgt tgggctcact ccagcacctg gaatggggtt gtatgttcaa     5100

gattacctct gaggtagaag gggtaagggg actaggagaa gttaaaacag cacaaagctt     5160

gctgttctta ccaaaactgg gatatttttc ttccataaat cctgtctaag gtgctgcaaa     5220

cctttggcta ttttgcaaaa ttctaaaaaa aaaaaaaaaa aaaaaaaaaa gttgattacg     5280

tcaaattttg cattatttca ctgatgttgt taaagggtaa atttctggag gttattatgt     5340

caccattttc actgatgtca cccattgcca ctttctttat gatacttccc aagtttctct     5400

caagagaatt aattactagg tctcagttag aagttagtaa aaataaaata tattttccca     5460

tccaagttga ctgactctct gaactctagc tacaggttcc ttcgagatct attaatcctg     5520

ggttaaaaac tctgtccttg attgatgtat gaaagatgaa caagcaacac aggacttacc     5580

agcatcatga cagaagtgtt gaggcaggtc caagtatgga cctgaatggc ctttacttac     5640

ttcctatttc cctagggcat taaactattc accaaagggt accaggtccc ttcaatcggt     5700

tcccttttcc ttgcagactc ctcaaagcta ctcaagacca agactgaggc acctacaacc     5760

atgacatacc ccttgaaagg tgagttttgc tagagtctga ggaagtagta gaggttcaaa     5820

atggtgtcat cgtagccaag aaaggaatca caatgccatg gaatggctaa taaacagtca     5880

cttggttcct cagagagaac atagcagtaa aggaatggaa acaaaagaaa ggtaacaatg     5940

atgaatatat actctttttt tgtgaaaact tctgaggcaa aaggaaagag agggttagaa     6000

ttactcccat tggaaataca aaaaacaaga acactaaatt ctattctgct cctagtttga     6060

cttgctgtgt gactttgggt acattacctc ccctcgatgg gaattcttct cactgcccac     6120

acctaacagt aagaaatgaa gatgccttat ctcatggtac tagtggttga taacatggtc     6180

aggtaccctg gaaagtacag cacaaatggc cagggaagga ctccaacctt tgcaaccatc     6240

tcctatctct caactttttg aaagaagaga gacatctctg accctattct ctatattcat     6300

agcaacatct acagtgaagc agtcctggga ctggaccact gacatggatg gctaccttgg     6360

agagaccagt gctgggccag gtaggaatat tgtcaggatt tctatctttt cttcttgact     6420

ctaagctttg tgaccaaaac tttcccttat gcacactgtt ctacagcatt gaaatctgca     6480

aatcattacc tgctatgtgc caagcaatgg tcaagggcac tgaggatggg taaagtgggt     6540

aggatgtaga gagagagata cagatccaga gatgagcaaa gtcagagtga ggttaagggg     6600

agtgataaat gtgcccttta tttagttctc tgttgtcata gtaatatgct ttccaaacat     6660

tctgagcaag aatatggata gggacattat gacatcatga cctcaaccag gtaaggataa     6720

tggctgagtg tcattgcttg agctcttaac cagggctctt caagccgctt gaagctgatt     6780

tctgattcct ccaaagcctt gaaaattccc aagtcagggt ctcagagatt caaatcccaa     6840

ataaatcttt tgtgtttcac ctgcatctga tagtcagttg gtatgccttc acatgactac     6900

attgactgtt gaaataatag aacactctca caccaattga taattagctg ctccccttaa     6960

cccctgagcc ctccattatt acctggggct gccttgtccc attcctcaga actctctaga     7020

ccacccccat ggtcatgcaa caaaaggtat accaaggaat ctctaagact ccaagtctac     7080

agtaggcatc tgttgtgact ggttggaatt aaatagtttg ataactatca ctcctggtac     7140

tcattctctg cctgtgtata attgagagag tggacttcca aatgctcttt gtaactctcc     7200

attcctgatt gcagagtttt ttggctatcc cttggcagca ctcaaacctg ttttatgttc     7260

cacatttgca ttgccatctt tggcttcaag atttccatag ctccgttcct ctccgagaag     7320

aacatctact gggagaagag aagttagaaa ttgcctcata gggagttggt ttggaaaaat     7380

gagaaaataa ccttgggcaa agttattaga tgttgggtct gcgccctatt ttctctacca     7440

agttttttag tacttgactc tcttcaaaag agaatatttc ttcgatttgt accagcttat     7500

aacttgagct ctctcagtcc caacaagaca ctctgaggac cacaaatact ggattcctag     7560

gacacgatat cttagagtct catgaaaaca agccagttgc agcaacaaat cctaagtaca     7620

ctgaggttcc tttcctcacg cctctctatc ccccctgacc tagcatctta gattcagaat     7680

attatagcta aaagtgcttt agacatcatc caatcttttc attgaactta tggggtaact     7740

aaggcctagt gacagtcaaa gttttgtcta aggtcactgt attcattttt ccatggctgc     7800

tgtaaaaact taccccaaac ctagtatctt aaaacagcat aaatatatta tcttccactt     7860

ctggaaataa gtctgacaca attatcactg agctaaaatc aaaatgtcag tcagcaaggc     7920

tatgttccct ttggagggtt tggagaagaa ttaatttcct tgtctttctt ttctggcttc     7980

tagaagctac ccacgttctt tggctcatgg cccccttcct ccattttcaa aactaacaat     8040

gtagcattct caaatctctg ttccttttta ctatgtaaac taacatactg acaggttcca     8100

ggaatttgga tgtgaatatc tttagaggac cattattctg cctaccatag tcatacagaa     8160

agtaaataac agatctagga ctggaagcca agtccagttg attctttcag cttttaggca     8220

taatgggaat atgtgtctca agtggctcag tggaaatatg ttttgagaaa gaaggaagct     8280

ggggacttga ccagagcgtg tgatgggaag aatctttgtg gctagaagta actgaccaga     8340

atcttaataa ggtatgtagc atttttggtg aagctggaaa gtcaggatat tattttcttt     8400

tttctaaggg aaggtgaaaa ccttctgtct tggccctata gaaagcatag acaactagct     8460

caaatgccca ggccaatcag cccaccagtg agtctggaag aacctgttta cacatggcat     8520

ctctttctca agtctttctc atgggactgg agttttttct ctctggctag ctaagaaggt     8580

taggagaata tggaaaatga aaaagaaaaa ggcctggctt agtctaggct gtttcttaga     8640

gtttttgttt ctggccttat tttcaatcaa ataccacttg atcttgtttt accctctagg     8700

aaagagc                                                               8707


<210>  6
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connection between the 5' end of the human VSIG4 sequence in the 
       A1 Fragment and the mouse sequence

<400>  6
gtctgtgttt gcctttttga ctttgttccc tgttttaggc cgtcccatcc tggaagtgcc       60

agagagtgta acaggacctt                                                   80


<210>  7
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connection between the 3' end of the human VSIG4 sequence and 
       mouse sequence

<400>  7
caaataccac ttgatcttgt tttaccctct aggaaagagc ctgccaatct ttgccataat       60

cttcatcatc tccctttgct                                                   80


<210>  8
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connection between the 5' end of the Neo cassette and the human 
       sequence

<400>  8
ttattagata ttttctttat atacatttca aatgctatcc gatatcgaat tccgaagttc       60

ctattctcta gaaagtatag gaa                                               83


<210>  9
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connection between the 3' end of the Neo cassette and the mouse 
       sequence

<400>  9
gaaagtatag gaacttcatc agtcaggtac ataatggtgg atccagtact tgaaagttcc       60

ctataccctc cctccgccct gctcccctac                                        90


<210>  10
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mRNA sequence of the engineered mouse VSIG4 after humanization

<400>  10
agctaccagc acttccaggt tcttcagcag caagaggatg gaaggatgaa tagaagtagc       60

ttcaaatagg atggagatct catcaggctt gctgttcctg ggccacctaa tagtgctcac      120

ctatggccgt cccatcctgg aagtgccaga gagtgtaaca ggaccttgga aaggggatgt      180

gaatcttccc tgcacctatg accccctgca aggctacacc caagtcttgg tgaagtggct      240

ggtacaacgt ggctcagacc ctgtcaccat ctttctacgt gactcttctg gagaccatat      300

ccagcaggca aagtaccagg gccgcctgca tgtgagccac aaggttccag gagatgtatc      360

cctccaattg agcaccctgg agatggatga ccggagccac tacacgtgtg aagtcacctg      420

gcagactcct gatggcaacc aagtcgtgag agataagatt actgagctcc gtgtccagaa      480

actctctgtc tccaagccca cagtgacaac tggcagcggt tatggcttca cggtgcccca      540

gggaatgagg attagccttc aatgccaggc tcggggttct cctcccatca gttatatttg      600

gtataagcaa cagactaata accaggaacc catcaaagta gcaaccctaa gtaccttact      660

cttcaagcct gcggtgatag ccgactcagg ctcctatttc tgcactgcca agggccaggt      720

tggctctgag cagcacagcg acattgtgaa gtttgtggtc aaagactcct caaagctact      780

caagaccaag actgaggcac ctacaaccat gacatacccc ttgaaagcaa catctacagt      840

gaagcagtcc tgggactgga ccactgacat ggatggctac cttggagaga ccagtgctgg      900

gccaggaaag agcctgccaa tctttgccat aatcttcatc atctcccttt gctgcatagt      960

agctgtcacc ataccttata tcttgttccg ctgcaggaca ttccaacaag agtatgtcta     1020

tggagtgagc agggtgtttg ccaggaagac aagcaactct gaagaaacca caagggtgac     1080

taccatcgca actgatgaac cagattccca ggctctgatt agtgactact ctgatgatcc     1140

ttgcctcagc caggagtacc aaataaccat cagatcaaca atgtctattc ctgcctgctg     1200

aacacagttt ccagaaacta agaagttctt gctactgaag aaaataacat ctgctaaaat     1260

gcccctacta agtcaaggtc tactggcgta attacctgtt acttatttac tacttgcctt     1320

caacatagct ttctccctgg cttcctttct tcttagacaa cctaaagtat ctatctagtc     1380

tgccaattct ggggccattg agaaatcctg ggtttggcta agaatatact acatgcacct     1440

caagaaatct agcttctggg cttcacccag aacaattttc ttcctagggc cttcacaact     1500

cttctccaaa cagcagagaa attccatagc agtagaggtt ctttatcatg cctccagaca     1560

gcgtgagtct cagtcctaca aactcagaca agcacatggg tctaggatta ctcctctttc     1620

tctagggcca gatgactttt aattgatatt actattgcta cattatgaat ctaatgcaca     1680

tgtattcttt tgttgttaat aaatgtttaa tcatgacatc aa                        1722


<210>  11
<211>  376
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  expressed protein sequence of the engineered mouse VSIG4

<400>  11

Met Glu Ile Ser Ser Gly Leu Leu Phe Leu Gly His Leu Ile Val Leu 
1               5                   10                  15      


Thr Tyr Gly Arg Pro Ile Leu Glu Val Pro Glu Ser Val Thr Gly Pro 
            20                  25                  30          


Trp Lys Gly Asp Val Asn Leu Pro Cys Thr Tyr Asp Pro Leu Gln Gly 
        35                  40                  45              


Tyr Thr Gln Val Leu Val Lys Trp Leu Val Gln Arg Gly Ser Asp Pro 
    50                  55                  60                  


Val Thr Ile Phe Leu Arg Asp Ser Ser Gly Asp His Ile Gln Gln Ala 
65                  70                  75                  80  


Lys Tyr Gln Gly Arg Leu His Val Ser His Lys Val Pro Gly Asp Val 
                85                  90                  95      


Ser Leu Gln Leu Ser Thr Leu Glu Met Asp Asp Arg Ser His Tyr Thr 
            100                 105                 110         


Cys Glu Val Thr Trp Gln Thr Pro Asp Gly Asn Gln Val Val Arg Asp 
        115                 120                 125             


Lys Ile Thr Glu Leu Arg Val Gln Lys Leu Ser Val Ser Lys Pro Thr 
    130                 135                 140                 


Val Thr Thr Gly Ser Gly Tyr Gly Phe Thr Val Pro Gln Gly Met Arg 
145                 150                 155                 160 


Ile Ser Leu Gln Cys Gln Ala Arg Gly Ser Pro Pro Ile Ser Tyr Ile 
                165                 170                 175     


Trp Tyr Lys Gln Gln Thr Asn Asn Gln Glu Pro Ile Lys Val Ala Thr 
            180                 185                 190         


Leu Ser Thr Leu Leu Phe Lys Pro Ala Val Ile Ala Asp Ser Gly Ser 
        195                 200                 205             


Tyr Phe Cys Thr Ala Lys Gly Gln Val Gly Ser Glu Gln His Ser Asp 
    210                 215                 220                 


Ile Val Lys Phe Val Val Lys Asp Ser Ser Lys Leu Leu Lys Thr Lys 
225                 230                 235                 240 


Thr Glu Ala Pro Thr Thr Met Thr Tyr Pro Leu Lys Ala Thr Ser Thr 
                245                 250                 255     


Val Lys Gln Ser Trp Asp Trp Thr Thr Asp Met Asp Gly Tyr Leu Gly 
            260                 265                 270         


Glu Thr Ser Ala Gly Pro Gly Lys Ser Leu Pro Ile Phe Ala Ile Ile 
        275                 280                 285             


Phe Ile Ile Ser Leu Cys Cys Ile Val Ala Val Thr Ile Pro Tyr Ile 
    290                 295                 300                 


Leu Phe Arg Cys Arg Thr Phe Gln Gln Glu Tyr Val Tyr Gly Val Ser 
305                 310                 315                 320 


Arg Val Phe Ala Arg Lys Thr Ser Asn Ser Glu Glu Thr Thr Arg Val 
                325                 330                 335     


Thr Thr Ile Ala Thr Asp Glu Pro Asp Ser Gln Ala Leu Ile Ser Asp 
            340                 345                 350         


Tyr Ser Asp Asp Pro Cys Leu Ser Gln Glu Tyr Gln Ile Thr Ile Arg 
        355                 360                 365             


Ser Thr Met Ser Ile Pro Ala Cys 
    370                 375     


<210>  12
<211>  701
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' homologous arm

<400>  12
ggctagactt gcttgcaaag tacatgtttc aaatctaata gctaatggtt tggttaaata       60

ggaatatact ccagaatttt tgcttcaccc tatgtttcat ctttagagtc ttctatagct      120

catctctttc accctgccag aaaagaattt gattttatgt cccaatcact gagcctttct      180

cttgaatgag tctgtttcaa gcagcaaact gtacaaaatg aagtactttg gtttccacta      240

ttcaccattt tccttcctac atatcatttg aaaatagtga aagaaaatat tatatatatg      300

tacatatatt tacatattct ataatatttt acatatatgc acttatatgt gtgtgcatac      360

atatacatgt aaaatattat agaattctaa aaattattct atcctaactc tcacataata      420

tttggaggaa aatggatcct tcacagtggt agtgacttac cgagggtcac ttactggcaa      480

gacagtgtcg tgaaaggagc ttaaaagcac tagagctaat aagtgttaaa gtctttgtcg      540

tagggactga ctcacattga tgggaaagtt aacagaccag aagagcacag ggatgaagga      600

agggtgtttc ccagaaacct attgagtctg tcactcatac cctcactcca gtgaacattt      660

tgtctgtgtt tgcctttttg actttgttcc ctgttttagg c                          701


<210>  13
<211>  2086
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' homologous arm

<400>  13
ctgccaatct ttgccataat cttcatcatc tccctttgct gcatagtagc tgtcaccata       60

ccttatatct tgttccgctg caggacattc caacaaggta actgataagt agttcaagtt      120

gtttttttgc ttattcattc ttttcaacat taccttgatc aacattctga tgatgctctg      180

tgtctaacat tccagtgagc ttgaagacca gttttagagt aatgatagag atggatgtta      240

attgtccctg caaacaatga tgtaagattt agagtaccca accaagcaca attatctttg      300

taagtcacct tataagaatg caagagaagc ttccctcaag ttgcttgcca tttatgtgtg      360

gatttccaag ttgaagcata gaagttatca acagcacaca tagcagaaag agtaatgatc      420

caaatgtaaa gaagaaaata ttgaacaaaa aataaagaga agtcaaagct ggaataatca      480

acaaagtttt tcagaagttt taagtagaac taggtcatat tacatgaaga caatatgtaa      540

agggacagta ttccaggaaa gtgaatccac atgagcataa gtacaagggt ctaaattgca      600

aatgactgtg gatcatcata aagaaaaatg atagtgaaat atagggaaga ataaaagttc      660

ctttctaatt agaggaggtt ttaaattcca gcctaaagaa tttcttcact gggttctgca      720

acatttctaa gattaactta gttgttttga tcagggaaac tgcctgatga agacaatatt      780

gtcaaagtta tttactgagt atgggaaaca cagggatgag ggaaattgga atgtttgctt      840

ctaaggctcc cttctcttta aataattcaa tttagtctgt agttactgag aagagtttgt      900

attatagatt ctgagtatat aaagacaaaa agaacagatg tggtgtacag aatttcccaa      960

tcagtgggga agacaaaaca tggaaattgg ggaggggggt cttatacgtt gcattgttag     1020

ctttcttgta atttagtaca tgaatttcca gttgctactc tgaaagatga taaccatctg     1080

gagggcagat ttaagtatca agaaatggtc atctttgtgc caatctaatc ctatggaata     1140

gagaacagtt ctccacagac actaagaagc aatgattatc taataatcgt ctttttaatt     1200

tttaagattt tatgtagtta tctttcatta attcaatgaa acatcaactt ttccatatat     1260

gatatatatg taacaactgc ccactttctt gaataacaga tgggcataag tgcacttaaa     1320

aatggattgg gctatggtcc ttgacatgga accaaattca atgcattttt cctgggctgt     1380

gactgggcaa tcaagcttcc cacagaattt tctgagaatc tttgatgaga ctactgggtg     1440

aagaacaagt cttgtgttct ccctttaccg acaacaagca acatcttcag cctgttgttc     1500

tgagtcaaaa atgccaaagg ggatgaaaat caaagaggat gtatacgttc tacttctcca     1560

acaaatctgc atccctgtat ttcaactcat gacaaaaacc tcttgatatt aaaaagctgt     1620

tacttggaat tctcttcagt ttcctccccc atctttgaat aggtactcag atttcatgtt     1680

tagatcttat atgctctcca tgtaattttc tgacatgaat cttccttatt ttttagagta     1740

tgtctatgga gtgagcaggt aagataccat ctcctattct aacttattaa ccattacctt     1800

ccctcaattc tgttggccat tgacagaaaa ccttgaaact agtcctaata gggtctttca     1860

gggtcagaaa ataatttact ctctcagata aatgtcttca tattatgtcc tggatcagtg     1920

aacacacaag atgatttagg tacaccaact ttctgttctc acagtccctg tctagcagga     1980

tcctgagatt cagtgaacaa gaacataggt ggtgggatgc tagaaaacca ggatctaagc     2040

cctaaaatct ttctcatgag taactaggtc ttggttccta gggtgt                    2086


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting site

<400>  14
agggtggggt ggcctaaaac agg                                               23


<210>  15
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting site

<400>  15
caggtccctg tcacactctc agg                                               23


<210>  16
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting site

<400>  16
gcaagttttg gtgaaatggc tgg                                               23


<210>  17
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting site

<400>  17
caggaatcac tatacatgtg agg                                               23


<210>  18
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting site

<400>  18
atcttcctac gtgactccac tgg                                               23


<210>  19
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting site

<400>  19
tgcatctatg atcccctgag agg                                               23


<210>  20
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting site

<400>  20
aaataagacc aagtgctatt tgg                                               23


<210>  21
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting site

<400>  21
gactttaata ccaacatacc agg                                               23


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting site

<400>  22
ggtcttgcta ctatcatacc agg                                               23


<210>  23
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting site

<400>  23
agctgttagt tatcctataa tgg                                               23


<210>  24
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting site

<400>  24
tgaccaagga ttatagtaac agg                                               23


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA targeting site

<400>  25
acataggctt ctctcaatat tgg                                               23


<210>  26
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  a DNA fragment containing the T7 promoter and sgRNA scaffold

<400>  26
gaattctaat acgactcact atagggggtc ttcgagaaga cctgttttag agctagaaat       60

agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct      120

tttaaaggat cc                                                          132


<210>  27
<211>  1332
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' homologous arm

<400>  27
cctggtgagg caaagaccat agatgccact tcagtgttca gatacattag tatatgcttt       60

ggtacttagc agtgtatcct gaaagtcaga taacataacc tcgctccatg cacaaggcat      120

cgtagtctac ctgcttaact gaaaatatgc taaataaaaa agattcagtc agtgatacct      180

tgcattttga aactatatct accaagaaag acattgccaa aaaacagccc ctggacccag      240

ttgaataaaa gtcatcaaga tatgagcata catatgaaaa cctctaatac aatgggcacc      300

catctctttt tgcctctgga ctccctccaa ctccccagcc catcaagaag caattttctt      360

tgttttcacc acacaatagc tagcacaacc agtgctagag agttactcag gttcttttct      420

tttccagacc aagtatggat gaaatctttt ccccctcagg atatcatttt ctaatatctt      480

tttggtaaga actaaaggaa atgaaaaaaa aaaaaaaaac aaagagaaaa gagaaataag      540

gacagaaatt ggctagactt gcttgcaaag tacatgtttc aaatctaata gctaatggtt      600

tggttaaata ggaatatact ccagaatttt tgcttcaccc tatgtttcat ctttagagtc      660

ttctatagct catctctttc accctgccag aaaagaattt gattttatgt cccaatcact      720

gagcctttct cttgaatgag tctgtttcaa gcagcaaact gtacaaaatg aagtactttg      780

gtttccacta ttcaccattt tccttcctac atatcatttg aaaatagtga aagaaaatat      840

tatatatatg tacatatatt tacatattct ataatatttt acatatatgc acttatatgt      900

gtgtgcatac atatacatgt aaaatattat agaattctaa aaattattct atcctaactc      960

tcacataata tttggaggaa aatggatcct tcacagtggt agtgacttac cgagggtcac     1020

ttactggcaa gacagtgtcg tgaaaggagc ttaaaagcac tagagctaat aagtgttaaa     1080

gtctttgtcg tagggactga ctcacattga tgggaaagtt aacagaccag aagagcacag     1140

ggatgaagga agggtgtttc ccagaaacct attgagtctg tcactcatac cctcactcca     1200

gtgaacattt tgtctgtgtt tgcctttttg actttgttcc ctgttttagg ccaccccacc     1260

ctaaaaacac ctgagagtgt gacagggacc tggaaaggag atgtgaagat tcagtgcatc     1320

tatgatcccc tg                                                         1332


<210>  28
<211>  1541
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' homologous arm

<400>  28
agaggctaca ggcaagtttt ggtgaaatgg ctggtaagac acggctctga ctccgtcacc       60

atcttcctac gtgactccac tggagaccat atccagcagg caaagtacag aggccgcctg      120

aaagtgagcc acaaagttcc aggagatgtg tccctccaaa taaataccct gcagatggat      180

gacaggaatc actatacatg tgaggtcacc tggcagactc ctgatggaaa ccaagtaata      240

agagataaga tcattgagct ccgtgttcgg aaatgtaagt cactgtgaga gtagaaatgc      300

attggtaagc aaagacacag tagagtagtg taatcccaga cacctctgag aaattgagtg      360

gccttggttg ctatgtacat aggttctctg taattctgtt tgctaatcac aagcacctca      420

gggtcaccac cttattagaa atttaaaaag tgtttaaaaa agacaattcc actaggctgt      480

ccattacttt ccaccctgta aggagtatct ttctactcac acagacccca ggtgggaagt      540

gctggttttc atatgacagt gttttgtgat ataaataata ccatatgata ggcaaaggct      600

tgtttgcatt tggagagcag gttttcattt ataagtattt ttgagaataa aaaataaaat      660

tttaaccggg caatggtggc acatgtcttt aatcccagca cttgggaggc agaggcaggc      720

agattctgag ttcgaggcta gcctggtcta caaggtgagt tccaggacag ccagggctat      780

atagagaaac tctgtcttgg aaaacaaaca aacaagcaaa caaataaata gataaataaa      840

taaatatata aataaatata aaatttgtaa atataaaaaa tacaattcca ttgccttata      900

aaatgctacc tatctctcct atattcacta aagtctttct gaggtacata cttcccacca      960

agtacatctc ccaatttacc acaaaacttt gcaaacagaa aagagcatta ttgtctacat     1020

aaagctaaac tctatccatt tatacccaac catataaatt tttctacagc agctgaccct     1080

gctgtccatt tctttctcct tcgtttaacc acttttactt gggttttcta cagtttatct     1140

gcatgactat tcctagatta ctttgcaggc tctaattcac tactttgtac atctagagta     1200

gaggacattg ttcacttcac ccagcttgag aattttcatt tctaatctta cagtctacac     1260

gttgtctctc tcttctctct ttaattattt ttttccaata cagatacttt ttagtcaatt     1320

tcaataccaa tgagaattta atgggcttct gtgtgttttt gaggttgtta ccagaaaatt     1380

attgcctcta attacatatt gaagcatttc cctatgcttg tttctaatat attgagagtt     1440

cccaacctca cattgaagtt cttgatctac ttttgttgat tttttacagg ataatacagg     1500

tcagatttga atattagtga tatccacttt ccccagcacc a                         1541


<210>  29
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P2A

<400>  29
ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct       60

ggacct                                                                  66


<210>  30
<211>  1200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequences of 83-1282 of NCBI accession number 
       NM_007268.3

<400>  30
atggggatct tactgggcct gctactcctg gggcacctaa cagtggacac ttatggccgt       60

cccatcctgg aagtgccaga gagtgtaaca ggaccttgga aaggggatgt gaatcttccc      120

tgcacctatg accccctgca aggctacacc caagtcttgg tgaagtggct ggtacaacgt      180

ggctcagacc ctgtcaccat ctttctacgt gactcttctg gagaccatat ccagcaggca      240

aagtaccagg gccgcctgca tgtgagccac aaggttccag gagatgtatc cctccaattg      300

agcaccctgg agatggatga ccggagccac tacacgtgtg aagtcacctg gcagactcct      360

gatggcaacc aagtcgtgag agataagatt actgagctcc gtgtccagaa actctctgtc      420

tccaagccca cagtgacaac tggcagcggt tatggcttca cggtgcccca gggaatgagg      480

attagccttc aatgccaggc tcggggttct cctcccatca gttatatttg gtataagcaa      540

cagactaata accaggaacc catcaaagta gcaaccctaa gtaccttact cttcaagcct      600

gcggtgatag ccgactcagg ctcctatttc tgcactgcca agggccaggt tggctctgag      660

cagcacagcg acattgtgaa gtttgtggtc aaagactcct caaagctact caagaccaag      720

actgaggcac ctacaaccat gacatacccc ttgaaagcaa catctacagt gaagcagtcc      780

tgggactgga ccactgacat ggatggctac cttggagaga ccagtgctgg gccaggaaag      840

agcctgcctg tctttgccat catcctcatc atctccttgt gctgtatggt ggtttttacc      900

atggcctata tcatgctctg tcggaagaca tcccaacaag agcatgtcta cgaagcagcc      960

agggcacatg ccagagaggc caacgactct ggagaaacca tgagggtggc catcttcgca     1020

agtggctgct ccagtgatga gccaacttcc cagaatctgg gcaacaacta ctctgatgag     1080

ccctgcatag gacaggagta ccagatcatc gcccagatca atggcaacta cgcccgcctg     1140

ctggacacag ttcctctgga ttatgagttt ctggccactg agggcaaaag tgtctgttaa     1200


<210>  31
<211>  660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequences of 95290668-95291327 of NCBI accession 
       number NC_000086.8

<400>  31
acacagtttc cagaaactaa gaagttcttg ctactgaaga aaataacatc tgctaaaatg       60

cccctactaa gtcaaggtct actggcgtaa ttacctgtta cttatttact acttgccttc      120

aacatagctt tctccctggc ttcctttctt cttagacaac ctaaagtatc tatctagtct      180

gccaattctg gggccattga gaaatcctgg gtttggctaa gaatatacta catgcacctc      240

aagaaatcta gcttctgggc ttcacccaga acaattttct tcctagggcc ttcacaactc      300

ttctccaaac agcagagaaa ttccatagca gtagaggttc tttatcatgc ctccagacag      360

cgtgagtctc agtcctacaa actcagacaa gcacatgggt ctaggattac tcctctttct      420

ctagggccag atgactttta attgatatta ctattgctac attatgaatc taatgcacat      480

gtattctttt gttgttaata aatgtttaat catgacatca acttcagttg tgttggtctt      540

ctctcaatac aataggacct atccgaaatt attaaaatga gacctatccg aaattattaa      600

aatgagacta aaatgctaag gacccctagg aatatagatc acgcactttg ctgtgctctc      660


<210>  32
<211>  1361
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  STOP

<400>  32
aggcctatta agggttccgg atcctcgggg acaccaaata tggcgatctc ggccttttcg       60

tttcttggag ctgggacatg tttgccatcg atccatctac caccagaacg gccgttagat      120

ctgctgccac cgttgtttcc accgaagaaa ccaccgttgc cgtaaccacc acgacggttg      180

ttgctaaaga agctgccacc gccacggcca ccgttgtagc cgccgttgtt gttattgtag      240

ttgctcatgt tatttctggc acttcttggt tttcctctta agtgaggagg aacataacca      300

ttctcgttgt tgtcgttgat gcttaaattt tgcacttgtt cgctcagttc agccataata      360

tgaaatgctt ttcttgttgt tcttacggaa taccacttgc cacctatcac cacaactaac      420

tttttcccgt tcctccatct cttttatatt ttttttctcg agggatcttt gtgaaggaac      480

cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg      540

taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat      600

tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg      660

aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc      720

aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag      780

aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta      840

tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg      900

taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac      960

acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt     1020

taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata     1080

atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc     1140

ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat     1200

aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg     1260

cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgacat     1320

ggtaagtaag cttgggctgc aggtcgaggg acctagtcga c                         1361


<210>  33
<211>  1995
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mRNA sequence of the engineered mouse VSIG4 after humanization

<400>  33
agctaccagc acttccaggt tcttcagcag caagaggatg gaaggatgaa tagaagtagc       60

ttcaaatagg atggagatct catcaggctt gctgttcctg ggccacctaa tagtgctcac      120

ctatggccac cccaccctaa aaacacctga gagtgtgaca gggacctgga aaggagatgt      180

gaagattcag tgcatctatg atcccctggg aagcggagct actaacttca gcctgctgaa      240

gcaggctgga gacgtggagg agaaccctgg acctatgggg atcttactgg gcctgctact      300

cctggggcac ctaacagtgg acacttatgg ccgtcccatc ctggaagtgc cagagagtgt      360

aacaggacct tggaaagggg atgtgaatct tccctgcacc tatgaccccc tgcaaggcta      420

cacccaagtc ttggtgaagt ggctggtaca acgtggctca gaccctgtca ccatctttct      480

acgtgactct tctggagacc atatccagca ggcaaagtac cagggccgcc tgcatgtgag      540

ccacaaggtt ccaggagatg tatccctcca attgagcacc ctggagatgg atgaccggag      600

ccactacacg tgtgaagtca cctggcagac tcctgatggc aaccaagtcg tgagagataa      660

gattactgag ctccgtgtcc agaaactctc tgtctccaag cccacagtga caactggcag      720

cggttatggc ttcacggtgc cccagggaat gaggattagc cttcaatgcc aggctcgggg      780

ttctcctccc atcagttata tttggtataa gcaacagact aataaccagg aacccatcaa      840

agtagcaacc ctaagtacct tactcttcaa gcctgcggtg atagccgact caggctccta      900

tttctgcact gccaagggcc aggttggctc tgagcagcac agcgacattg tgaagtttgt      960

ggtcaaagac tcctcaaagc tactcaagac caagactgag gcacctacaa ccatgacata     1020

ccccttgaaa gcaacatcta cagtgaagca gtcctgggac tggaccactg acatggatgg     1080

ctaccttgga gagaccagtg ctgggccagg aaagagcctg cctgtctttg ccatcatcct     1140

catcatctcc ttgtgctgta tggtggtttt taccatggcc tatatcatgc tctgtcggaa     1200

gacatcccaa caagagcatg tctacgaagc agccagggca catgccagag aggccaacga     1260

ctctggagaa accatgaggg tggccatctt cgcaagtggc tgctccagtg atgagccaac     1320

ttcccagaat ctgggcaaca actactctga tgagccctgc ataggacagg agtaccagat     1380

catcgcccag atcaatggca actacgcccg cctgctggac acagttcctc tggattatga     1440

gtttctggcc actgagggca aaagtgtctg ttaaacacag tttccagaaa ctaagaagtt     1500

cttgctactg aagaaaataa catctgctaa aatgccccta ctaagtcaag gtctactggc     1560

gtaattacct gttacttatt tactacttgc cttcaacata gctttctccc tggcttcctt     1620

tcttcttaga caacctaaag tatctatcta gtctgccaat tctggggcca ttgagaaatc     1680

ctgggtttgg ctaagaatat actacatgca cctcaagaaa tctagcttct gggcttcacc     1740

cagaacaatt ttcttcctag ggccttcaca actcttctcc aaacagcaga gaaattccat     1800

agcagtagag gttctttatc atgcctccag acagcgtgag tctcagtcct acaaactcag     1860

acaagcacat gggtctagga ttactcctct ttctctaggg ccagatgact tttaattgat     1920

attactattg ctacattatg aatctaatgc acatgtattc ttttgttgtt aataaatgtt     1980

taatcatgac atcaa                                                      1995


<210>  34
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connection between the 3' end of the Neo cassette and the mouse 
       sequence

<400>  34
ttctctagaa agtataggaa cttcatcagt caggtacata atggtggatc ccttaatata       60

tttctgtgaa atgttccaaa atctgatatt g                                      91


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA6 upstream sequence

<400>  35
tgcatctatg atcccctgag                                                   20


<210>  36
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA6 forward oligonucleotide

<400>  36
taggtgcatc tatgatcccc tgag                                              24


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA6 downstream sequence

<400>  37
ctcaggggat catagatgca                                                   20


<210>  38
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA6 reverse oligonucleotide

<400>  38
aaacctcagg ggatcataga tgca                                              24


<210>  39
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  39
ctgaatttct gcttgcttca tcttcc                                            26


<210>  40
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  40
cttctttagc aacaaccgtc gtgg                                              24


<210>  41
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  41
gtgctctcag gcctattaag ggttcc                                            26


<210>  42
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  42
ccagcacaat actgctataa aaaga                                             25


<210>  43
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  43
cgtagggact gactcacatt gatgg                                             25


<210>  44
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  44
cagggtattt atttggaggg acacatc                                           27


<210>  45
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  45
ggtccagggt tctcctccac g                                                 21


<210>  46
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  46
cttctcacga tcaacccaga acag                                              24


<210>  47
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  47
gcataaacat agcccatcat tgctg                                             25


<210>  48
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  48
aactgatgaa tgggagcagt ggtgg                                             25


<210>  49
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  49
gcagacactc tatgcctgtg tggag                                             25


<210>  50
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  50
aagtgagcca caaagttcca gg                                                22


<210>  51
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  51
tctggttcat cagttgcgat gg                                                22


<210>  52
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  52
ttggtgaagt ggctggtaca ac                                                22


<210>  53
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  53
atgcagggct catcagagta gt                                                22


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  54
accacagtcc atgccatcac                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  55
tccaccaccc tgttgctgta                                                   20


<210>  56
<211>  3731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' homologous arm

<400>  56
tgtctggttg tctttagtga acatgactat ataaaagtaa tgccttgttt atacctacat       60

tacagaatgt acagtgaaaa attaaagaga tagtaaatgt caggtacaag cactatagag      120

acaaaagaag aaatgtaaga gaatagagat gaaacatata aagatacaag agtaagttca      180

ctaatactca ttttagtaca gtgtctgaat gaataaaaga tggaaaatgc cagttgttat      240

tgctgtagcc gatattgtat agataatgtg gcttgatcat ttttaattta caacacacaa      300

ttaccaagtc tcatcatgtt catgttacaa ctgagaaagc taatattcag aagggtttta      360

tttttctaag gcctagaatt aaaactttca atctaataaa gcattccctt attaagtcta      420

acaaactaca aataaggctt atctttctct ttaaaaaaat aaaatataga tgtctttaca      480

aaggcatcat gaggaatact aattaaagct agtcgtttct tgacatcaga gatggggcta      540

ctggcaaaag tcagtcaaca aactttccca ctgtgagata tgcgacaccc tccaaagcaa      600

ctatatccct agtcttaaca actaataaaa caatttctgt gtagaaagtc atccagtgca      660

ctaaactcat actatgaagc actcgagtgc ctctaattca cacagtgtcc atttgtattg      720

aggactatgg agctaatggt acaaattatg aaaactgcat atgccagaac aaatggtttc      780

cctagactga ccagaaagta aaacatccct ctatggtaaa ctttgagcag actcaaaatt      840

ctgggacatt aaagaccaga gagcaccact ggtcccagag aaagtcaaat cctagtagaa      900

tgaggaacag tttcacccat ccttaaatct ctaaccttac gcagcgtaaa aagaaagaac      960

cctgtgtgac tcttctgaaa tacggaaggt gggggaagtg tgtgaggaaa ctaggattct     1020

gagggaataa aatagaaaag caacacagaa actggcagct accagcactt ccaggttctt     1080

cagcagcaag aggatggaag gatgaataga agtagcttca aataggatgg agatctcatc     1140

aggcttgctg ttcctgggcc acctaatagt gctcacctat ggtaaggcag gatattgata     1200

cttgactacc tcttcaccct ctggcagaaa atgtgagtgt tgactttgag tgtttaccga     1260

gaaaaacttg tttctcctgg gcagtcacag ttgcctgcaa ttctacaggc tttctgaatg     1320

ttgagcagaa gggaagaaag tctggtaaag cttccagaaa ctgccttgga tagaaattga     1380

aagagttctt ctcttagaaa atatctgcat cactttccaa tatttcaggg atgtcagagg     1440

actccaatgt catgcaaact ttccatattc tccttttccc ttgaaatttt ataattatga     1500

ctaactttcc atttccaagc tttgattctt gctgcagtat atacctccac aatgaataaa     1560

tgttgttgag taaatagatg tgaccttaga agtaacctat agccaaggat gttgaggtac     1620

acctttaatc ctagctgttg ggaggtagag gcactctgat ctctgtgatc tcaaggacac     1680

tttcgactac atagaaagtt ccaggatatc taaggttaca tggtgagact cagtttgtaa     1740

ataaatagat aaaagtaaca catgccacta cttagaattg tttacaagtg ctcagcactt     1800

gctattcata cacttttaga acacagacat ttactgatat ccatgctact ctaagacttc     1860

tcacgatcaa cccagaacag atactattcc ttttttttca tgtcagcact tcaaacatct     1920

aaagaacgga aagaactgta tcctcttaat tttcttctct aaactaaata tctctagctt     1980

ctttaactaa aggatgtggt atactactag ctgctgggat aactcacttc cctgtgggca     2040

tagtagccca gttgtagggc ctgtttgcag ggatctaatt ctagattcat agtccagcag     2100

agtctgggat atctcatagt agcattattt ctgtgactct tcttccactc tcactattca     2160

agaaaatgaa atgccttttc aggaaactaa gtacaagtaa taactactta agacttggta     2220

tagggtaaaa ggattttttt gctgaatttc tgcttgcttc atcttcctat agggtgtcat     2280

tggcttaaac ataatttgaa gtactcagca atgatgggct atgtttatgc tttttaattt     2340

tattttattg aagttgaccc ctctaatggt taaaatatgc cgtgtatttt ccaagagttc     2400

ctggtgaggc aaagaccata gatgccactt cagtgttcag atacattagt atatgctttg     2460

gtacttagca gtgtatcctg aaagtcagat aacataacct cgctccatgc acaaggcatc     2520

gtagtctacc tgcttaactg aaaatatgct aaataaaaaa gattcagtca gtgatacctt     2580

gcattttgaa actatatcta ccaagaaaga cattgccaaa aaacagcccc tggacccagt     2640

tgaataaaag tcatcaagat atgagcatac atatgaaaac ctctaataca atgggcaccc     2700

atctcttttt gcctctggac tccctccaac tccccagccc atcaagaagc aattttcttt     2760

gttttcacca cacaatagct agcacaacca gtgctagaga gttactcagg ttcttttctt     2820

ttccagacca agtatggatg aaatcttttc cccctcagga tatcattttc taatatcttt     2880

ttggtaagaa ctaaaggaaa tgaaaaaaaa aaaaaaaaca aagagaaaag agaaataagg     2940

acagaaattg gctagacttg cttgcaaagt acatgtttca aatctaatag ctaatggttt     3000

ggttaaatag gaatatactc cagaattttt gcttcaccct atgtttcatc tttagagtct     3060

tctatagctc atctctttca ccctgccaga aaagaatttg attttatgtc ccaatcactg     3120

agcctttctc ttgaatgagt ctgtttcaag cagcaaactg tacaaaatga agtactttgg     3180

tttccactat tcaccatttt ccttcctaca tatcatttga aaatagtgaa agaaaatatt     3240

atatatatgt acatatattt acatattcta taatatttta catatatgca cttatatgtg     3300

tgtgcataca tatacatgta aaatattata gaattctaaa aattattcta tcctaactct     3360

cacataatat ttggaggaaa atggatcctt cacagtggta gtgacttacc gagggtcact     3420

tactggcaag acagtgtcgt gaaaggagct taaaagcact agagctaata agtgttaaag     3480

tctttgtcgt agggactgac tcacattgat gggaaagtta acagaccaga agagcacagg     3540

gatgaaggaa gggtgtttcc cagaaaccta ttgagtctgt cactcatacc ctcactccag     3600

tgaacatttt gtctgtgttt gcctttttga ctttgttccc tgttttaggc caccccaccc     3660

taaaaacacc tgagagtgtg acagggacct ggaaaggaga tgtgaagatt cagtgcatct     3720

atgatcccct g                                                          3731


<210>  57
<211>  3583
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' homologous arm

<400>  57
cttaatatat ttctgtgaaa tgttccaaaa tctgatattg taatgatttc agccttctat       60

gtaactgcac atctaaatac tgtaaccatt taaaaattac atttgtctaa ttaatcttga      120

gttgtaagtg taaattattt atttggaatg ttatagtctt atgaaataca ttagtgtatt      180

tctccatcct tcatattgtc tttttaaaat tttttttaga tttatttata tgtgagtgtt      240

ttgtctacag gtattcccat gcaccatatg tatgtctagt acttaaagag gtctgaatag      300

tgatcaaatg ctctctatct ggagttatag atgtttataa gccatcatgt gtgtgctaac      360

aaaaccaaac ccaggtcctc tacaagagca gacagtgatc ttaactatta agccatctct      420

ccaaccaaac atgttgcctt tttattttca tgatagtatc ctttaaggtc ccaacgtttc      480

taattttatt gacacctaac ctatatttgt cttagtactt gtatgttgaa ctatgtccta      540

ccaaacattt cttaacatgc ataaagtctg gcagaaatga gtagtgaagg cacatgggct      600

tgatattttc tttgttagta gttgtttaac tagcaatcca aatgtttttt tacttgttta      660

aattatgtaa ttttatttcc tggttcatct tttaaaatta acatttctgt ttttaagaat      720

ttatcatttt atacatataa gtgttttatc taaatatatg tgtgtcttag ttactttttt      780

attactgtga taaaatgatt taacaaactt atgaagtaaa tagtttgagc ttacaatttc      840

ttttatttgc taatttcttt tttttattag gtatttattt catttacatt tccaatgctt      900

tcccaaaagt cccacacatg ctccctaccc actcccccgc ccacccactc ccaattcttg      960

gccctggcgt tcccctgtac taaggcatat aaagtttgca agaccaatgg gcctctcttt     1020

ccagtgatgg ccgactaggc catcttctga tacatatgca gctagagaca agagctccgg     1080

gggtactggt tagttcatat tgctcttcca tctatagggt tgcagatccc tttagctcct     1140

tggttacttt ctctagctcc tccattgggc gccctgtgat acatccaata gctgactgtg     1200

agcatccact tatgtgtttg aaggccccgg catagtctca caagagacag caatatctgg     1260

gtcctttcag caagatcttg ctaatatatg caatggtgtt agtgtttgga agctgattat     1320

gggatggatc cccggatatg gcagcctcta gatggtccat cctttcatct cagctccaaa     1380

ctttgtctct gtaactcctt ccatgggtgt tttgttccca attctaagac gggacaaagt     1440

gctcacattt tggtcttggt tcttcttgag tttcatgtgt ttcacaaatt ttatcttata     1500

tcttgggtat tctaaatttc tgagctaata tccacttatc agtaagtaca tatggtttga     1560

gttcttttgt gattggatta cctcactcag gatgatgccc tccaggtcca tccatttgcc     1620

taggaatttc ataaattcat tcttttgaac agctgagtag tactccattg tgtaaatgta     1680

cctcattttc tgtatccatt cctctgttga ggggcatctg ggttctttcc agcttctggc     1740

tattataaat aaggctgcta tgaacatcgt agagcatgtg tccttcttac caattggaac     1800

atcttctgga tatatgccaa ggagaggtat tgcaggatcc tctggtagta ctatgtcaaa     1860

ttttctgagg aactaccaga ctgatttcca aagtggttgt aaaagcttgc aatctcacca     1920

gcaatggagg agtgtttctc tttctccaca tcctcgtcag catctactgt gacatgaatt     1980

tttgatctta gccattctga ctggtgtgag gtagaatctc agggttgttt tgacttgcat     2040

ttccctgatg attaaggatg ctgaacattt tttcaggtgc ttctcagcca ttcggtattc     2100

ctcacgtgag aattcttctg tttttttttt ttttgtttgt ttgtttgttt gtttctgttt     2160

ttgttttttt tttggctctg agccccattt tttaataggg ttatttgatt tttctggagt     2220

ccaccttctt aagttcttta tatatattgg atattagtcc cctatctgat ttaggatagg     2280

taaagatcct ttcccaatct gttggtggcc tttttgtctt attgacagta acttttgcct     2340

tgcagaaact ttgcaatttt ttgaggtccc attagtcaat tctcgatctt acagcacaag     2400

ccattgctgt tctattcagg aatttttccc ctttcttcga ggtttttccc tactttctcc     2460

tcaataagtt tcagtgtctc tggttttatg tggagttcct taatccactt agatttgacc     2520

ttagtacaag gagataggaa tggatcaatt tgcattcttc tacatgataa ccgccagttg     2580

tgccagtacc atttgttgaa aatgccgtct tttttccacc agaaggtttt tgctcccttg     2640

tcaaagatca agtgaccata ggtgtgtggg ttcatttctg ggtcttcaat tctattccat     2700

tggtctactt gtctgtctct ataccagtac catgcagttt ttatcacaat tgctctgtag     2760

tacagcttta ggtcaggcat ggtgattcca ccggaggttc ttttatcctt gagcagagtt     2820

tttgctatcc taggcttttt gttattccag atgaatttac agattgccct ttctaatttg     2880

ttgaagaact gatttgtaat tttgatgggg attgcattga atctgtaaat tgcttttggc     2940

aagatagaca tttttactat attaatcctg ccaatccatg agcatgtgag atctttccat     3000

cttctgagat cttctttaat ttctttcttc agagatttga agtttttatc ataaagatct     3060

ttcacttcct tagttagagt caaaccaagg tattttatat tatttgtgac tattgagaag     3120

ggtattgttt ccctaattaa tttctcagtc tatttatcct ttgtgtacag aaaggccatt     3180

gacttgtttc agttaatttt atatccagca aattccctga agctgtttat caggtttagg     3240

aggtctctgg tagaattttt aggatcactt atatatgcta tcatatcatc tgcaaaaagt     3300

gatattttga ctttttcctt gccaatttgt atccccttga tctgctattg ttatcgaatt     3360

gctctggcta gaacttcaag tacaatgttg aataggtatg gagaaagtga gcagccttgt     3420

gtattccctg gttttagtgg gattgcttcc agcttctcac catttacttt gatggtggct     3480

actcgtttgc tgtaaattgc ttttatcatg tttaggtatg ggcctccaat gcctgatctt     3540

tccaagactt taatcatgaa tgggtgttgg attttgtcaa atg                       3583


<210>  58
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connection between the STOP sequence and the mouse sequence

<400>  58
gtaagtaagc ttgggctgca ggtcgaggga cctagtcgac agaggctaca ggcaagtttt       60

ggtgaaatgg ctggtaagac                                                   80


<210>  59
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connection between the 5' end of the Neo cassette and the mouse 
       sequence

<400>  59
ctgacctgtg tctttttata gcagtattgt gctggttttg gaattccgaa gttcctattc       60

tctagaaagt ataggaactt caggt                                             85


<210>  60
<211>  285
<212>  PRT
<213>  Rattus norvegicus

<400>  60

Met Glu Ile Ser Ser Ser Leu Leu Phe Leu Ser His Leu Ile Val Leu 
1               5                   10                  15      


Thr Tyr Gly His Pro Val Leu Lys Thr Pro Glu Arg Val Thr Gly Thr 
            20                  25                  30          


Trp Lys Gly Asp Val Lys Ile Gln Cys Ile Tyr Asn Pro Leu Thr Gly 
        35                  40                  45              


Tyr Arg Glu Val Leu Val Lys Trp Leu Val Gln Arg Gly Ser Asp His 
    50                  55                  60                  


Val Thr Ile Phe Leu Arg Asp Tyr Ser Gly Asp His Val Gln Gln Ala 
65                  70                  75                  80  


Lys Tyr Arg Gly Arg Leu Lys Val Ser His Lys Val Pro Gly Asp Val 
                85                  90                  95      


Ser Leu Gln Leu Asn Asn Leu Gln Met Asp Asp Arg Asn His Tyr Thr 
            100                 105                 110         


Cys Glu Val Thr Trp Gln Thr Pro Asp Gly Asp Gln Val Arg Arg Asp 
        115                 120                 125             


Lys Ile Thr Glu Leu Arg Val Gln Lys Tyr Pro Tyr Lys Pro Pro Lys 
    130                 135                 140                 


Ile Thr Thr Glu Ala Leu Thr Thr Met Gln Ser Ser Leu Glu Ala Thr 
145                 150                 155                 160 


Thr Ile Thr Ser Ser Thr Ser Asp Trp Thr Thr Ser Val Thr Gln Thr 
                165                 170                 175     


Pro Gln Gly Thr Thr Thr Val Pro Gly Gly Lys Leu Pro Ile Phe Ala 
            180                 185                 190         


Ile Ile Leu Ile Ile Cys Phe Cys Cys Ile Val Val Val Thr Ile Ala 
        195                 200                 205             


Phe Ile Met Phe Arg Arg Arg Thr Phe Gln Glu Tyr Val Tyr Glu Val 
    210                 215                 220                 


Ser Arg Met Tyr Ala Arg Gln Thr Ser Asn Ser Glu Glu Thr Thr Arg 
225                 230                 235                 240 


Val Thr Thr Ile Ala Ser Ala Tyr Phe Asn Asn Glu Pro Asp Ser Gln 
                245                 250                 255     


Ala Leu Ile Asn Asp Tyr Ser Asp Asp Pro Cys Leu Ser Gln Glu Tyr 
            260                 265                 270         


Gln Ile Thr Thr Arg Ser Thr Met Thr Ile Pro Ala Cys 
        275                 280                 285 


