                         SEQUENCE LISTING

<110>  Ĵײ̩ҽҩɷ޹˾
       ˼ҩҵ޹˾
 
<120>  ҩżＰƱ;

<130>  IEC210174PCT

<150>  202110615214.X
<151>  2021-06-02

<150>  202110941134.3
<151>  2021-8-16

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  118
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 VH

<400>  1

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Tyr Ser Ile Thr Ser Asp 
            20                  25                  30          


Tyr Ala Trp Asn Trp Ile Arg Gln Phe Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Gly Tyr Ile Ser Tyr Ser Gly Ser Ile Arg Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Val Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Phe Ser 
65                  70                  75                  80  


Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Glu Ile Thr Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  2
<211>  107
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 VL

<400>  2

Glu Thr Thr Leu Thr Gln Ser Pro Ala Phe Met Ser Ala Thr Pro Gly 
1               5                   10                  15      


Asp Lys Val Thr Ile Ser Cys Lys Ala Ser Thr Ser Ile Asp Asp Asp 
            20                  25                  30          


Met Asn Trp Tyr Gln Gln Lys Pro Gly Glu Pro Pro Lys Leu Ile Ile 
        35                  40                  45              


Ser Glu Gly Asn Thr Leu Arg Pro Gly Ile Pro Pro Arg Phe Ser Ser 
    50                  55                  60                  


Ser Gly Tyr Gly Thr Asp Phe Thr Phe Thr Ile Asn Lys Ile Lys Ser 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Leu Gln Ser Asn Asp Leu Pro Leu 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  3
<211>  8
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 Chothia CDR-H1

<400>  3

Gly Tyr Ser Ile Thr Ser Asp Tyr 
1               5               


<210>  4
<211>  5
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 Chothia CDR-H2

<400>  4

Ser Tyr Ser Gly Ser 
1               5   


<210>  5
<211>  9
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 Chothia/AbM/Kabat CDR-H3

<400>  5

Gly Glu Ile Thr Tyr Tyr Phe Asp Tyr 
1               5                   


<210>  6
<211>  11
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 AbM/Kabat/Chothia CDR-L1

<400>  6

Lys Ala Ser Thr Ser Ile Asp Asp Asp Met Asn 
1               5                   10      


<210>  7
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 AbM/Kabat/Chothia CDR-L2

<400>  7

Glu Gly Asn Thr Leu Arg Pro 
1               5           


<210>  8
<211>  9
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 AbM/Kabat/Chothia /IMGT CDR-L3

<400>  8

Leu Gln Ser Asn Asp Leu Pro Leu Thr 
1               5                   


<210>  9
<211>  121
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24 VH

<400>  9

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Ser Ile Asn Ser Gly Gly Ser Thr Tyr Tyr Pro Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Val Asn Tyr Tyr Gly Tyr Gly Asp Tyr Ala Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120     


<210>  10
<211>  107
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24 VL

<400>  10

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Asp Ile Ser Asn Tyr 
            20                  25                  30          


Leu Thr Trp Phe Gln Gln Lys Pro Gly Lys Ser Pro Lys Thr Leu Ile 
        35                  40                  45              


His Arg Ala Asn Arg Leu Val Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Leu Gln Tyr Asp Glu Phe Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  11
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24 Chothia CDR-H1

<400>  11

Gly Phe Thr Phe Ser Ser Tyr 
1               5           


<210>  12
<211>  5
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24 Chothia CDR-H2

<400>  12

Asn Ser Gly Gly Ser 
1               5   


<210>  13
<211>  13
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24 AbM/Kabat/Chothia CDR-H3

<400>  13

Val Asn Tyr Tyr Gly Tyr Gly Asp Tyr Ala Met Asp Tyr 
1               5                   10              


<210>  14
<211>  11
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24/38F8_Hu57 AbM/Kabat/Chothia CDR-L1

<400>  14

Gln Ala Ser Gln Asp Ile Ser Asn Tyr Leu Thr 
1               5                   10      


<210>  15
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24/38F8_Hu57 AbM/Kabat/Chothia CDR-L2

<400>  15

Arg Ala Asn Arg Leu Val Thr 
1               5           


<210>  16
<211>  9
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24/38F8_Hu57 IMGT/Abm/Kabat/Chothia CDR-L3

<400>  16

Leu Gln Tyr Asp Glu Phe Pro Tyr Thr 
1               5                   


<210>  17
<211>  120
<212>  PRT
<213>  ˹

<220>
<223>  38F8_Hu57 VH

<400>  17

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Ser Ile Ser Ser Gly Gly Pro Thr Tyr Tyr Pro Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Val Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Gly Tyr Arg Tyr Asp Val Asn Trp Tyr Phe Asp Val Trp Gly Ala 
            100                 105                 110         


Gly Thr Met Val Thr Val Ser Ser 
        115                 120 


<210>  18
<211>  107
<212>  PRT
<213>  ˹

<220>
<223>  38F8_Hu57 VL

<400>  18

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Asp Ile Ser Asn Tyr 
            20                  25                  30          


Leu Thr Trp Phe Gln Gln Lys Pro Gly Lys Ser Pro Lys Thr Leu Ile 
        35                  40                  45              


His Arg Ala Asn Arg Leu Val Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Ile Tyr Tyr Cys Leu Gln Tyr Asp Glu Phe Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  19
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  38F8_Hu57 Chothia CDR-H1

<400>  19

Gly Phe Thr Phe Ser Asn Tyr 
1               5           


<210>  20
<211>  5
<212>  PRT
<213>  ˹

<220>
<223>  38F8_Hu57 Chothia CDR-H2

<400>  20

Ser Ser Gly Gly Pro 
1               5   


<210>  21
<211>  12
<212>  PRT
<213>  ˹

<220>
<223>  38F8_Hu57 AbM/Kabat/Chothia CDR-H3

<400>  21

Gly Tyr Arg Tyr Asp Val Asn Trp Tyr Phe Asp Val 
1               5                   10          


<210>  22
<211>  330
<212>  PRT
<213>  ˹

<220>
<223>  Human IgG1㶨

<400>  22

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  23
<211>  107
<212>  PRT
<213>  ˹

<220>
<223>  Human kappa㶨

<400>  23

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  24
<211>  9
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 IMGT CDR-H1

<400>  24

Gly Tyr Ser Ile Thr Ser Asp Tyr Ala 
1               5                   


<210>  25
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 IMGT CDR-H2

<400>  25

Ile Ser Tyr Ser Gly Ser Ile 
1               5           


<210>  26
<211>  11
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 IMGT CDR-H3

<400>  26

Ala Arg Gly Glu Ile Thr Tyr Tyr Phe Asp Tyr 
1               5                   10      


<210>  27
<211>  6
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 IMGT CDR-L1

<400>  27

Thr Ser Ile Asp Asp Asp 
1               5       


<210>  28
<211>  3
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 IMGT CDR-L2

<400>  28

Glu Gly Asn 
1           


<210>  29
<211>  11
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 AbM CDR-H1

<400>  29

Gly Tyr Ser Ile Thr Ser Asp Tyr Ala Trp Asn 
1               5                   10      


<210>  30
<211>  9
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 AbM CDR-H2

<400>  30

Tyr Ile Ser Tyr Ser Gly Ser Ile Arg 
1               5                   


<210>  31
<211>  6
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 Kabat CDR-H1

<400>  31

Ser Asp Tyr Ala Trp Asn 
1               5       


<210>  32
<211>  16
<212>  PRT
<213>  ˹

<220>
<223>  19F6_Hu35V1 Kabat CDR-H2

<400>  32

Tyr Ile Ser Tyr Ser Gly Ser Ile Arg Tyr Asn Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  33
<211>  8
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24 IMGT CDR-H1

<400>  33

Gly Phe Thr Phe Ser Ser Tyr Ala 
1               5               


<210>  34
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24 IMGT CDR-H2

<400>  34

Ile Asn Ser Gly Gly Ser Thr 
1               5           


<210>  35
<211>  15
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24 IMGT CDR-H3

<400>  35

Ala Arg Val Asn Tyr Tyr Gly Tyr Gly Asp Tyr Ala Met Asp Tyr 
1               5                   10                  15  


<210>  36
<211>  10
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24 AbM CDR-H1

<400>  36

Gly Phe Thr Phe Ser Ser Tyr Ala Met Ser 
1               5                   10  


<210>  37
<211>  9
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24 AbM CDR-H2

<400>  37

Ser Ile Asn Ser Gly Gly Ser Thr Tyr 
1               5                   


<210>  38
<211>  5
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24 Kabat CDR-H1

<400>  38

Ser Tyr Ala Met Ser 
1               5   


<210>  39
<211>  16
<212>  PRT
<213>  ˹

<220>
<223>  3D8_HuC24 Kabat CDR-H2

<400>  39

Ser Ile Asn Ser Gly Gly Ser Thr Tyr Tyr Pro Asp Ser Val Lys Gly 
1               5                   10                  15      


<210>  40
<211>  8
<212>  PRT
<213>  ˹

<220>
<223>  38F8_Hu57 IMGT CDR-H1

<400>  40

Gly Phe Thr Phe Ser Asn Tyr Ala 
1               5               


<210>  41
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  38F8_Hu57 IMGT CDR-H2

<400>  41

Ile Ser Ser Gly Gly Pro Thr 
1               5           


<210>  42
<211>  14
<212>  PRT
<213>  ˹

<220>
<223>  38F8_Hu57 IMGT CDR-H3

<400>  42

Ala Arg Gly Tyr Arg Tyr Asp Val Asn Trp Tyr Phe Asp Val 
1               5                   10                  


<210>  43
<211>  6
<212>  PRT
<213>  ˹

<220>
<223>  38F8_Hu57 /3D8_HuC24 IMGT CDR-L1

<400>  43

Gln Asp Ile Ser Asn Tyr 
1               5       


<210>  44
<211>  3
<212>  PRT
<213>  ˹

<220>
<223>  38F8_Hu57/3D8_HuC24 IMGT CDR-L2

<400>  44

Arg Ala Asn 
1           


<210>  45
<211>  10
<212>  PRT
<213>  ˹

<220>
<223>  38F8_Hu57 AbM  CDR-H1

<400>  45

Gly Phe Thr Phe Ser Asn Tyr Ala Met Ser 
1               5                   10  


<210>  46
<211>  9
<212>  PRT
<213>  ˹

<220>
<223>  38F8_Hu57 AbM  CDR-H2

<400>  46

Ser Ile Ser Ser Gly Gly Pro Thr Tyr 
1               5                   


<210>  47
<211>  5
<212>  PRT
<213>  ˹

<220>
<223>  38F8_Hu57 Kabat CDR-H1

<400>  47

Asn Tyr Ala Met Ser 
1               5   


<210>  48
<211>  16
<212>  PRT
<213>  ˹

<220>
<223>  38F8_Hu57 Kabat CDR-H2

<400>  48

Ser Ile Ser Ser Gly Gly Pro Thr Tyr Tyr Pro Asp Ser Val Lys Gly 
1               5                   10                  15      


