                         SEQUENCE LISTING

<110>  DANISCO US INC
 
<120>  COMPOSITIONS AND METHODS FOR ENHANCED PROTEIN PRODUCTION IN 
       BACILLUS CELLS

<130>  NB41871-US-PSP

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  483
<212>  PRT
<213>  Cytophaga sp.

<400>  1

Ala Ala Thr Asn Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr Val Pro 
1               5                   10                  15      


Asn Asp Gly Gln Gln Trp Asn Arg Leu Arg Thr Asp Ala Pro Tyr Leu 
            20                  25                  30          


Ser Ser Val Gly Ile Thr Ala Val Trp Thr Pro Pro Ala Tyr Lys Gly 
        35                  40                  45              


Thr Ser Gln Ala Asp Val Gly Tyr Gly Pro Tyr Asp Leu Tyr Asp Leu 
    50                  55                  60                  


Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr Lys 
65                  70                  75                  80  


Gly Glu Leu Lys Ser Ala Val Asn Thr Leu His Ser Asn Gly Ile Gln 
                85                  90                  95      


Val Tyr Gly Asp Val Val Met Asn His Lys Ala Gly Ala Asp Tyr Thr 
            100                 105                 110         


Glu Asn Val Thr Ala Val Glu Val Asn Pro Ser Asn Arg Tyr Gln Glu 
        115                 120                 125             


Thr Ser Gly Glu Tyr Asn Ile Gln Ala Trp Thr Gly Phe Asn Phe Pro 
    130                 135                 140                 


Gly Arg Gly Thr Thr Tyr Ser Asn Trp Lys Trp Gln Trp Phe His Phe 
145                 150                 155                 160 


Asp Gly Thr Asp Trp Asp Gln Ser Arg Ser Leu Ser Arg Ile Phe Lys 
                165                 170                 175     


Phe His Gly Lys Ala Trp Asp Trp Pro Val Ser Ser Glu Asn Gly Asn 
            180                 185                 190         


Tyr Asp Tyr Leu Met Tyr Ala Asp Tyr Asp Tyr Asp His Pro Asp Val 
        195                 200                 205             


Val Asn Glu Met Lys Lys Trp Gly Val Trp Tyr Ala Asn Glu Val Gly 
    210                 215                 220                 


Leu Asp Gly Tyr Arg Leu Asp Ala Val Lys His Ile Lys Phe Ser Phe 
225                 230                 235                 240 


Leu Lys Asp Trp Val Asp Asn Ala Arg Ala Ala Thr Gly Lys Glu Met 
                245                 250                 255     


Phe Thr Val Gly Glu Tyr Trp Gln Asn Asp Leu Gly Ala Leu Asn Asn 
            260                 265                 270         


Tyr Leu Ala Lys Val Asn Tyr Asn Gln Ser Leu Phe Asp Ala Pro Leu 
        275                 280                 285             


His Tyr Asn Phe Tyr Ala Ala Ser Thr Gly Gly Gly Ala Tyr Asp Met 
    290                 295                 300                 


Arg Asn Ile Leu Asn Asn Thr Leu Val Ala Ser Asn Pro Thr Lys Ala 
305                 310                 315                 320 


Val Thr Leu Val Glu Asn His Asp Thr Gln Pro Gly Gln Ser Leu Glu 
                325                 330                 335     


Ser Thr Val Gln Pro Trp Phe Lys Pro Leu Ala Tyr Ala Phe Ile Leu 
            340                 345                 350         


Thr Arg Ser Gly Gly Tyr Pro Ala Val Phe Tyr Gly Asp Met Tyr Gly 
        355                 360                 365             


Thr Lys Gly Thr Thr Thr Tyr Glu Ile Pro Ala Leu Lys Ser Lys Ile 
    370                 375                 380                 


Glu Pro Leu Leu Lys Ala Arg Lys Asp Tyr Ala Tyr Gly Thr Gln Arg 
385                 390                 395                 400 


Asp Tyr Ile Asp Asn Pro Asp Val Ile Gly Trp Thr Arg Glu Gly Asp 
                405                 410                 415     


Ser Thr Lys Ala Lys Ser Gly Leu Ala Thr Val Ile Thr Asp Gly Pro 
            420                 425                 430         


Gly Gly Ser Lys Arg Met Tyr Val Gly Thr Ser Asn Ala Gly Glu Ile 
        435                 440                 445             


Trp Tyr Asp Leu Thr Gly Asn Arg Thr Asp Lys Ile Thr Ile Gly Ser 
    450                 455                 460                 


Asp Gly Tyr Ala Thr Phe Pro Val Asn Gly Gly Ser Val Ser Val Trp 
465                 470                 475                 480 


Val Gln Gln 
            


<210>  2
<211>  9515
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic expression cassette

<400>  2
ctgggagctg atagcacagc gatttcccgc ctcctgtcgg aagcatcgcc accgtgtccc       60

gtccctccag cacgcttttg atgatcgttt cctggccttt tttaaaagca tgataaccaa      120

aatacctgcg gagcgcctga tgaagtttat ccattcctga tcaccgtttt cgacagggcc      180

aggcggattt taaaataact gtatcggccg ccgagtccct ctttaatttg tctgatttta      240

ttggtttggt tcgctatcgc ataatcggca ataattttct gttcctcttt cgacacgtac      300

tgatcgatgg aaaaagacgg atcatgaatg gcgatttcca caatgtgatc ctcgatcgtc      360

gccaatttca gctttctaat ttttgcgatc cggtctattg tatacccttc ttttataaga      420

aagagcgttt ttctcgtcga ttgtgtcaga ccatcattca aaggaatgtc atgaatgagc      480

gtttgaaaaa gcggactttc accattttgg gcggattgaa taaaatggtg cagaacatcc      540

caaaacagtg catatatgta ccactcatca aggttcattt tttcggaaag ctgtctgaat      600

gtatagcccg cttttgttct ggatgtcagg gagtgaacaa aaatagcggc ctgttcatca      660

tgatttaaaa cagacagctt ctctttcagc tcttggtgaa actgggccgc tgtttcagca      720

gcattccggt ttcttaaata ctgtttaacc cagttctgaa tctgataatc ttttacgatc      780

ggcaaataca cgcgttcgcg gtaaagcttg ttggagagca cttggatcaa aagcgacatc      840

cttgcccaca tcactttggc tgccgcctga taatagccgc cgtgaaaatg gcggggccac      900

ggataaaggg caaaaaagcc ggcaagttcc gcttctcctt tttcggtgac ggtgtaagcg      960

ccgctttccg ctttttctct gaccaaagac tcctgcttca atcgttgaac gctggctgcc     1020

acctgctctc tcgacagagc cgaacaaaag ccgaagtatt ttgaaacggc aaataaaccg     1080

gcgtcctgta tcgtctgtga cgaccttttt ccttttaata aatgatagac cgcgcttgga     1140

gaacgctcac ccttcatgga tgacagaatg tcaagcacaa tcgcgtcaaa aaaatgaacc     1200

ggcatatcat cacctgcaat cttccggcaa cattcgatca tttcttcctt ttattttaac     1260

agattttgcg gagaaatcga cgtttaaact catataaaag gggtatgtta gcagtagaac     1320

ccttgtgtga taagcattct caatattttt gagttgaaat gtaagattaa caccattaca     1380

ataaggaatg ggaataggtt tcatatcgga tagatagagg gttaaaccat ttgttccaac     1440

gaagaacaat ctgggaggtt ttttattcat gccaaaatat acaattgtag acaaagatac     1500

gtgcatcgca tgcggagctt gtggtgctgc ggctcctgat atttatgatt acgacgatga     1560

gggaatcgca tttgtcaccc ttgacgacaa tcagggtgtc gtcgaagtcc ctgacgtctt     1620

agaagaagac atgatggacg cgtttgaagg ctgtcctaca gattcgatca aagttgcgga     1680

tgagccgttc gaaggcgacc cgcttaaaca cgaataaagc caaaaaacat ccggtgcaca     1740

aagtgccgga tgttttttta tgagataagc acggctttac caacaagcaa aaagaagccg     1800

gctaaagaca tccggcttct tctgcagctg acaatatccg ggaacatgca cccgatattg     1860

tcatgtttat ttatttggcc atgcggacgt tttccttcag ccgcggtttc agcgaaagga     1920

aaatcggcgt ggacacgagg gccacagcga tgcctttaat gaaattaaaa ggcaggattc     1980

cggccagaac tgttgtcttg agcgcctctc cagtcagcgc tggagcattt aaaaaccaag     2040

tgtaggcagg cagaaacagc agataattta aaatgctcat cgaaacggcc atcacaagcg     2100

tccctgcgaa aagagctgtg acaaaccctt tggcagaact tgattttttc agcagtacag     2160

ctgccggcag gataaacaat gttccggcaa tgaagttagc cgcctgatca atcggaacgc     2220

ccgaggcgct tcctgcaata aagtaattca gcacgttttt gatcgcttca acggcaatcc     2280

cggctcccgg accgtacaaa ataacagcga gcaatgccgg gatatcactg aaatcgattt     2340

ttaaatacgg gaatgccccc aggatcggaa agctcagcat cattaaaata aatgcgatgc     2400

tgctcagcat gctgatagag acgagacgtc tcaccttgtt gtgtttcatt ttgtcactct     2460

ctccttttcg atcacatctc acgaaaagag gaatggttct ttcccctgtc ctaaacaaaa     2520

aacccgcttt attgaaaaag cggggctgtt ttacagacag gtcaaataaa cgtttgaaaa     2580

tgttcatttc aaaacgcgcg gaacctccat cttctcccat ccagactata ctgtcggctt     2640

cggaatcgca ccgaatcctg cccataaaaa ggctcgcggg cttagagcgc ttgctcatca     2700

ccgccggtag ggaatttcac cctgccccga agattgatct tatttatttt taatactgat     2760

attattataa attaattgtg aaaaaatgta caggtgcaaa gcttattgcg ctgttttggg     2820

acatcctgca cgatatttcg gtaaactcac tttttccgca tactaaaaac cgcacattca     2880

cagttatttc atttttaatt ttcgtctttc cgcgtgaaac tcattgacac tctttatgga     2940

atatggtaaa ttatcagata tttatgacgc ttatttagga ggaaatctta catgtttcga     3000

gtattggtct cagataaaat gtccagcgac ggcctcaaac cattaatgga agcagatttt     3060

attgaaattg tagaaaagaa tgttgcggaa gcggaagacg agcttcatac gtttgacgcg     3120

ctcttggtgc ggagcgccac gaaggtaacc gaagagctgt ttaaaaagat gacttcgctg     3180

aaaatcgtcg ccagagcagg tgtcggcgtc gacaatatcg atattgacga ggcgacaaaa     3240

cacggtgtta tcgtcgtaaa cgcgccaaac gggaatacaa tttcaaccgc tgaacatacc     3300

tttgcaatgt tttcagcgtt aatgagacat attccgcagg caaacatctc cgtgaaatca     3360

agggagtgga atcgttcggc ttacgtcggt tcagagcttt acggaaaaac gctcggcatc     3420

atcggaatgg gccgcatcgg aagcgaaatc gcgagccgcg caaaagcatt cggtatgacc     3480

gttcatgtat ttgacccgtt cctgacccaa gaaagggcaa gcaagctcgg cgttaacgcg     3540

aacagctttg aagaagttct ggcatgcgcc gacatcatta cggttcatac cccgctcacg     3600

aaagaaacga agggactttt gaacaaagaa accatcgcaa aaacgaaaaa aggcgttcgt     3660

ctcgttaact gtgcaagagg cggcatcatc gatgaagcag cgcttttgga agctctggaa     3720

agcggacatg tcgctggcgc tgccttggat gtattcgaag tcgagcctcc ggtcgattca     3780

aaactgatcg atcatccgct tgtagtcgcg actcctcact tgggcgcctc aacaaaagaa     3840

gcccagctga atgtcgctgc acaagtgtcc gaagaagtcc ttcagtatgc gcaaggaaac     3900

cctgtgatgt ccgcgatcaa ccttccggcc atgacaaagg attcattcga aaaaatccag     3960

ccttatcatc agtttgccaa tacgatcgga aaccttgtgt ctcagtgcat gaatgagcct     4020

gttcaagatg tagccatcca atatgaaggc tccatcgcca aacttgaaac gtcatttatt     4080

acgaaaagcc ttttggccgg atttctgaag ccgagggtcg cggctaccgt taacgaagtg     4140

aatgccggca ccgttgcgaa agagcgcggc atcagcttca gcgaaaaaat ttcttccaat     4200

gagtcaggct atgaaaactg catctctgtg actgtcacgg gagatgtaac aacattctct     4260

ttaagagcga cgtacattcc gcacttcggc ggacgcatcg ttgccttaaa cggctttgat     4320

attgattttt atccggctgg acaccttgtc tacattcacc accaggataa accaggggct     4380

atcggccatg tcggacgaat tttaggagac catgacatca atatcgccac tatgcaggta     4440

ggccgaaaag aaaaaggcgg agaagcgatc atgatgcttt cctttgaccg ccaccttgag     4500

gacgatattt tagctgagct gaaaaacatc ccggatatcg tgtctgttaa agccatcgac     4560

cttccttaag tcgctgataa acagctgaca tcaatatcct attttttcaa aaaatatttt     4620

aaaaagttgt tgacttaaaa gaagctaaat gttatagtaa taaaacagaa tagtctttta     4680

agtaagtcta ctctgaattt ttttaaaagg agagggtaaa gatgaaacaa caaaaacggc     4740

tttacgcccg attgctgacg ctgttatttg cgctcatctt cttgctgcct cattctgcag     4800

ctagcgcagc agcgacaaac ggaacaatga tgcagtattt cgagtggtat gtacctaacg     4860

acggccagca atggaacaga ctgagaacag atgcccctta cttgtcatct gttggtatta     4920

cagcagtatg gacaccgccg gcttataagg gcacgtctca agcagatgtg gggtacggcc     4980

cgtacgatct gtatgattta ggcgagttta atcaaaaagg tacagtcaga acgaagtatg     5040

gcacaaaagg agaacttaaa tctgctgtca acacgctgca ttcaaatgga atccaagtgt     5100

atggtgatgt cgtgatgaat cataaagcag gtgctgatta tacagaaaac gtaacggcgg     5160

tggaggtgaa tccgtctaat agatatcagg aaacgagcgg cgaatataat attcaggcat     5220

ggacaggctt caactttccg ggcagaggaa caacgtattc taactggaaa tggcagtggt     5280

tccattttga tggaacggat tgggaccaga gcagaagcct ctctagaatc ttcaaattcc     5340

atggaaaggc gtgggactgg ccggtttctt cagaaaacgg aaattatgac tatctgatgt     5400

acgcggacta tgattatgac catccggatg tcgtgaatga aatgaaaaag tggggcgtct     5460

ggtatgccaa cgaagttggg ttagatggat acagacttga cgcggtcaaa catattaaat     5520

ttagctttct caaagactgg gtggataacg caagagcagc gacgggaaaa gaaatgttta     5580

cggttggcga atattggcaa aatgatttag gggccctgaa taactacctg gcaaaggtaa     5640

attacaacca atctcttttt gatgcgccgt tgcattacaa cttttacgct gcctcaacag     5700

ggggtggagc gtacgatatg agaaatattc ttaataacac gttagtcgca agcaatccga     5760

caaaggctgt tacgttagtt gagaatcatg acacacagcc tggacaatca ctggaatcaa     5820

cagtccaacc gtggtttaaa ccgttagcct acgcgtttat tctcacgaga agcggaggct     5880

atcctgcggt attttatgga gatatgtacg gtacaaaagg aacgacaaca tatgagatcc     5940

ctgctcttaa atctaaaatc gaacctttgc ttaaggctag aaaagactat gcttatggaa     6000

cacagagaga ctatattgat aacccggatg tcattggctg gacgagagaa ggggactcaa     6060

cgaaagccaa gagcggtctg gccacagtga ttacagatgg gccgggcggt tcaaaaagaa     6120

tgtatgttgg cacgagcaat gcgggtgaaa tctggtatga tttgacaggg aatagaacag     6180

ataaaatcac gattggaagc gatggctatg caacatttcc tgtcaatggg ggctcagttt     6240

cagtatgggt gcagcaatga aagcttctcg aggttaacag aggacggatt tcctgaagga     6300

aatccgtttt tttattttac agaagctgcg gaacctgaaa agaattcctt tcaggttccg     6360

ttttttttag gaattctccc tgatctcaag catctggcgg ggataaatcc gctctccttt     6420

caaatcgttc cattctttga ggcgctgtac agttacgccc attttttcgg cgatatgatg     6480

aagcgtatcc cctttccgca ctacatatgt accggtcttc gattcatcgt catgaaggcg     6540

gagtgtttgg ccggccttga gatttgaatg tttcaacccg tttattctca tgatctcctc     6600

gatggatata ccgctatcct tgctgattct ccagagcgtg tccccttttt gaacggtcac     6660

cgcaccgctc attgtcccgg cgttttgata aacgtggata gaattttgcc ggaacgcctc     6720

ctcacgaagc accgtcagcg gattgattgc atatctttta tcttcagtcc atgaaccgtg     6780

atgcatttca aaatgcaggt gggttccggt cgatattccc gtattgccga tgattccgat     6840

ttgctcgcct tttttcaccc gctccttttc ctttttcagg cgtttgctta agtgggcata     6900

aacggtttca tatccgttgt catgtttaat aaatatcact tggccgtagg agtcggattg     6960

atacgatttg cttatcgttc cgtctgcggc tgccgctact gcttcccctt cgggagcagc     7020

gatgtcaagc cccttatgct ttccgcctct cgtaccgaat tgatctgtga tctctccttt     7080

aatcggttca atccactctg aggcttccgc ccccggggca ttgacgaaaa gcgccaatcc     7140

cgaaagccat gcgatcgcga acaggaagtt ttgatgtctg agtttcttca aggttttcca     7200

tatcctccta ttacatgcat cttcggtaaa attgccccct attcggagac agcttagtat     7260

acttccaaat caatacaatt tatacattaa aaaaagactc cgcacaggga gtcttttagt     7320

tttctatcgt catcggattc ggtgcgtacg gaacctgtac agatttcgac aggtcatagg     7380

cgccgacctt ggttatggat gcgtttttaa atttcacttt tgtgaagccg aaatctttcg     7440

cggtcaatag aaggccttcc accatcaaga catcttcggg tttattttca atattcgcgg     7500

aggaagaaaa ttgaatgatc agttcttttc cattcttttg aatatcttca atcggcgtat     7560

catcggataa aatgggtttt aaatgagtgc cgctttcttc gtttttcatc atcttaatcg     7620

cttcctgcac cgattcgtaa gattcgcttg aaggtgcaag gaaccggcgc ccgtctgagc     7680

tttcatataa atagtagcat ttttgcgtct ggtgcataat cgccatatcg gcgagcattc     7740

cgaatgtttc aaattcaaca cccgatttat cattggaaat aaacagaaca gaatcatacg     7800

atccccattt aaaggtttcg ttgatcacat ttttcagccg ttcgaaatct tcgactgata     7860

gctccggtat tttctcatca acttgaatct tcagtttttt attgtttttc tgctctttga     7920

acttcacctt atcaaggtaa gctgtgtcaa atgatgtaaa ctggtccact ccaagccggc     7980

tgtaagcgtg aagcgcatct tcaagatttg tcatgccagt gcttttctcg aggcttaccg     8040

ggacaacgac agacttggac tcgtcaagga aagcgaaggt gatatagtcg tctttttgat     8100

tctgtgagac gacaaacgta tttgcaggtt cagacttggc agcatcagcc tccgtctgca     8160

ccaattttcc gtcagaagaa atgttggcgt cggcgctgtt ttgagatctg atctgttcga     8220

ttaactgggg agtgatcagc atcagaagaa agagaaccaa aactgtagca gcaaatgcgc     8280

cgacccgttt tttcggtgat ttacgctttg gtgcgagaat cagcttttga tagatctgat     8340

ttgccgaacg attatcctta accgttggaa gttggcttag taacgccttc agccgttctt     8400

cgttccattc tgacttcttc attctttgga tcctccttca aaagctccat ctgtttacga     8460

agcactttca gaccgcggtg ctgagtggtt tttaccttgc tttcggaaaa attcaaggct     8520

tttgctgttt cactgatcga atatccttga ataaaacgca agacgataac tgatctttgg     8580

tcaagcgtac acttgtctag ggcctcgaaa atttccttta ggttttcatt ttgcatcacg     8640

atgtcctcag gcagaggctt gcggtctttt acatcttgtt tctcccagtc aaacgtcccc     8700

aaaatccgct ggcggatcgt ctgctgcttt ctgaaccagt cgatcgcaac gtgccgcgca     8760

atcgaaagaa gccaggtttt ttcgctgctc ctgccttcaa atgtttcgta agaatgcagg     8820

acgcggatgt atacttcctg aactaagtct tccgcctgat ttttgtcttt taccatataa     8880

aataaaaact gaaataaatc ctgatgatac tgatcatata ttttctgaaa ggtttcttcc     8940

acctgaaacc cctccgttca atttattgtc gtttgtcaat cttaaaaggt tacattacaa     9000

ctattacaac tatattacga acatatgaaa atggaaaggg ggttttgcga aagttaagct     9060

taattttaac ttaacaagca caaaagcacc cgttctaaat gaacaggtgc caaggttata     9120

ggagcccaca ttttcactaa gctgtgccct tacaaggctt tcgttctcct gaccggagcg     9180

ttgcggatcc gctgaaatga actaatttca atccgtttat gactttaagt ccaattgttg     9240

gcgaagcttt ttggaaatct ccattctctt ttcgtcagtc actaggtgat accataagcc     9300

gtcaactttt ttgtcttcac cttcaatttc tacctgctta atatctttag cagcttcttt     9360

ataagaactt tgaatatcga tcatcttgtc cagtgttaag tttgttttga tgtttttttc     9420

taatgctgcc aaaatctctt gatagtttgt taatgatttt aattgtgcac cttcatgaat     9480

aatttcgttg ataatctcac gctgacgctc ttgac                                9515


<210>  3
<211>  1000
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  serA1 locus

<400>  3
aatggttctt tcccctgtcc taaacaaaaa acccgcttta ttgaaaaagc ggggctgttt       60

tacagacagg tcaaataaac gtttgaaaat gttcatttca aaacgcgcgg aacctccatc      120

ttctcccatc cagactatac tgtcggcttc ggaatcgcac cgaatcctgc ccataaaaag      180

gctcgcgggc ttagagcgct tgctcatcac cgccggtagg gaatttcacc ctgccccgaa      240

gattgatctt atttattttt aatactgata ttattataaa ttaattgtga aaaaatgtac      300

aggtgcaaag cttattgcgc tgttttggga catcctgcac gatatttcgg taaactcact      360

ttttccgcat actaaaaacc gcacattcac agttatttca tttttaattt tcgtctttcc      420

gcgtgaaact cattgacact ctttatggaa tatggtaaat tatcagatat ttatgacgct      480

tatttaggag gaaatcttac acagaagctg cggaacctga aaagaattcc tttcaggttc      540

cgtttttttt aggaattctc cctgatctca agcatctggc ggggataaat ccgctctcct      600

ttcaaatcgt tccattcttt gaggcgctgt acagttacgc ccattttttc ggcgatatga      660

tgaagcgtat cccctttccg cactacatat gtaccggtct tcgattcatc gtcatgaagg      720

cggagtgttt ggccggcctt gagatttgaa tgtttcaacc cgtttattct catgatctcc      780

tcgatggata taccgctatc cttgctgatt ctccagagcg tgtccccttt ttgaacggtc      840

accgcaccgc tcattgtccc ggcgttttga taaacgtgga tagaattttg ccggaacgcc      900

tcctcacgaa gcaccgtcag cggattgatt gcatatcttt tatcttcagt ccatgaaccg      960

tgatgcattt caaaatgcag gtgggttccg gtcgatattc                           1000


<210>  4
<211>  1578
<212>  DNA
<213>  Bacillus licheniformis

<400>  4
atgtttcgag tattggtctc agataaaatg tccagcgacg gcctcaaacc attaatggaa       60

gcagatttta ttgaaattgt agaaaagaat gttgcggaag cggaagacga gcttcatacg      120

tttgacgcgc tcttggtgcg gagcgccacg aaggtaaccg aagagctgtt taaaaagatg      180

acttcgctga aaatcgtcgc cagagcaggt gtcggcgtcg acaatatcga tattgacgag      240

gcgacaaaac acggtgttat cgtcgtaaac gcgccaaacg ggaatacaat ttcaaccgct      300

gaacatacct ttgcaatgtt ttcagcgtta atgagacata ttccgcaggc aaacatctcc      360

gtgaaatcaa gggagtggaa tcgttcggct tacgtcggtt cagagcttta cggaaaaacg      420

ctcggcatca tcggaatggg ccgcatcgga agcgaaatcg cgagccgcgc aaaagcattc      480

ggtatgaccg ttcatgtatt tgacccgttc ctgacccaag aaagggcaag caagctcggc      540

gttaacgcga acagctttga agaagttctg gcatgcgccg acatcattac ggttcatacc      600

ccgctcacga aagaaacgaa gggacttttg aacaaagaaa ccatcgcaaa aacgaaaaaa      660

ggcgttcgtc tcgttaactg tgcaagaggc ggcatcatcg atgaagcagc gcttttggaa      720

gctctggaaa gcggacatgt cgctggcgct gccttggatg tattcgaagt cgagcctccg      780

gtcgattcaa aactgatcga tcatccgctt gtagtcgcga ctcctcactt gggcgcctca      840

acaaaagaag cccagctgaa tgtcgctgca caagtgtccg aagaagtcct tcagtatgcg      900

caaggaaacc ctgtgatgtc cgcgatcaac cttccggcca tgacaaagga ttcattcgaa      960

aaaatccagc cttatcatca gtttgccaat acgatcggaa accttgtgtc tcagtgcatg     1020

aatgagcctg ttcaagatgt agccatccaa tatgaaggct ccatcgccaa acttgaaacg     1080

tcatttatta cgaaaagcct tttggccgga tttctgaagc cgagggtcgc ggctaccgtt     1140

aacgaagtga atgccggcac cgttgcgaaa gagcgcggca tcagcttcag cgaaaaaatt     1200

tcttccaatg agtcaggcta tgaaaactgc atctctgtga ctgtcacggg agatgtaaca     1260

acattctctt taagagcgac gtacattccg cacttcggcg gacgcatcgt tgccttaaac     1320

ggctttgata ttgattttta tccggctgga caccttgtct acattcacca ccaggataaa     1380

ccaggggcta tcggccatgt cggacgaatt ttaggagacc atgacatcaa tatcgccact     1440

atgcaggtag gccgaaaaga aaaaggcgga gaagcgatca tgatgctttc ctttgaccgc     1500

caccttgagg acgatatttt agctgagctg aaaaacatcc cggatatcgt gtctgttaaa     1560

gccatcgacc ttccttaa                                                   1578


<210>  5
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic promoter

<400>  5
gtcgctgata aacagctgac atcaatatcc tattttttca aaaaatattt taaaaagttg       60

ttgacttaaa agaagctaaa tgttatagta ataaa                                  95


<210>  6
<211>  58
<212>  DNA
<213>  Bacillus subtilis

<400>  6
acagaatagt cttttaagta agtctactct gaattttttt aaaaggagag ggtaaaga         58


<210>  7
<211>  87
<212>  DNA
<213>  Bacillus licheniformis

<400>  7
atgaaacaac aaaaacggct ttacgcccga ttgctgacgc tgttatttgc gctcatcttc       60

ttgctgcctc attctgcagc tagcgca                                           87


<210>  8
<211>  34
<212>  DNA
<213>  Bacillus licheniformis

<400>  8
cggatttcct gaaggaaatc cgttttttta tttt                                   34


<210>  9
<211>  1000
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  lysA locus

<400>  9
tcgttccggg ttcgatgccg cctccatgga tggccatgat gagcatcggc actcttgcgt       60

tcttctccgt aattcggtaa gaagctggat cttcattccg ctccaattct tcaaagcttg      120

aatattgatc gccggactgt gttgcgccat gggccgcagc agaatatggg aatgagatca      180

cagccgaaat caataaaata ttcacaaaaa tgtttattgc tgtaaaacga tttactgtgt      240

tacaatcaac acattgcttc aggcactgga gcaatcggtg gagtagggat actttggccg      300

gtttcatcta ctccgcctct aaatccaaca aatcagcaaa tctcatcctt ttcaccctgg      360

tcattgcctt tatcatctcc tttgtaaatt ttttgttaaa ttcacaatat tatatacaat      420

tagcccggtc gctgttctat gcatgtcatg atctttttaa caaaaaacac ctcccgatca      480

ccgggaagtg tttttcgtgc ttgtcattcc ctctttctct atcgacaaag gtgcaagggg      540

cgctccggtt gctgcccgat gcatcttccg gcttcatgat agtgcgattc ttatgttgta      600

ttataacatt tcgttttaaa aactaaacca ccatttgtcc aaattttctt gatttataaa      660

ggaaatgatt ccctaaacaa ctaatccatt ctattgatta tagaataaca gaaaacagcg      720

cttgatagaa ataggcatac acaacgtcca ttcaataaaa aaagagccga aaagctgaga      780

ttttcgccca tacaaatagg catttacctg aatataatct taaagatgaa aaccaaaaaa      840

cgaagtggga aagaggttat catatgtatc caaatcgcta tccctacacc cccgctcccg      900

gctatgcacc tgaagatgta tacgcccctt atcagccggc tgcgcattat gaacagagaa      960

tttatgagcc ttatgaaggg cagattatta cagctccggc                           1000


<210>  10
<211>  1320
<212>  DNA
<213>  Bacillus licheniformis

<400>  10
ttgtttttac acggtactag cagacaaaat gaaagagggc acctcgaaat cggcggtgtc       60

gatgttctat cattggcaga aagatacgga acacctcttt atgtatacga tgtcgcgctg      120

attagagagc gcgcccgaaa attccagaag gcattcaagg aagccggttt aaaagcgcag      180

gtagcgtatg caagcaaggc gttttcatcg gttgccatga ttcagcttgc cgaacaagag      240

gggctgtctc tggatgtggt atcgggagga gagcttttca ctgcgatcaa agcagggttc      300

ccagctgagc ggattcattt tcacggaaac aataagagcc ctgaagaact agccatggcg      360

ctggagcatc aaatcggctg catcgtgctc gataactttc acgagatcgc cattacagaa      420

gatctttgca agcgatcagg acaaactgta gacgttttgc tcagaatcac tccgggagtt      480

gaagcgcaca cgcacgatta tattacgacg gggcaggaag attccaaatt cggttttgat      540

ctgcataatg gacaggtcga acaagccatc gaacaagtcc tccgctcgtc tgcgtttaag      600

ctcctcggcg tgcactgcca catcggttcg caaatttttg atacggcagg atttgtcctt      660

gcagcagaca agattttcga gaagcttgcg gaatggcggg agacttactc tttcattccg      720

gaagtgctca atcttggcgg gggcttcggc atccgctata caaaagacga cgagccgctt      780

gcagctgatg tttatgttga aaaaatcatc gaggcggtca aagcaaatgc cgagcatttc      840

ggctttgaca tccctgagat ttggatcgaa ccaggccggt ctctcgtcgg tgatgcgggg      900

actacgctgt acacgatcgg ttctcaaaaa gaggtgccgg gcattcgcaa atatgtagcc      960

atcgacggcg gcatgagcga taatatcagg ccggcgcttt atgaggcaaa atatgaagca     1020

gccgtcgcca acaggatgaa cgatgcttgt catgataccg catcaatcgc aggaaaatgc     1080

tgcgaaagcg gagatatgct gatttgggat ttggaaatcc ccgaagttcg cgacggagat     1140

gtgctcgccg ttttctgcac cggtgcgtac ggctacagca tggccaacaa ctacaaccgc     1200

attccgcgcc cggccgtcgt ctttgtcgag gacggggaag cgcagctcgt cattcagaga     1260

gagacgtatg aggatatcgt caagctggat ctgccgctga aatcgaaagt caaacaataa     1320


<210>  11
<211>  74
<212>  DNA
<213>  Bacillus licheniformis

<400>  11
gcttttcttt tggaagaaaa tatagggaaa atggtacttg ttaaaaattc ggaatattta       60

tacaatatca tatg                                                         74


<210>  12
<211>  5822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette

<400>  12
aaatataagg aggccaatta aatgatgaca gcaaaaaatt tgcaagatcg aatcaaattg       60

aataacggga cagctatgcc ttggtttgga cttggcgtat ttaaagtaga agaagggccc      120

gaactggtac aagccgtaaa aacggcgatt aaacacggct accgcagtat tgataccgca      180

gccatctacg gtaatgaaga gggggttggg caaggaatcc gcgaggggtt gaaagaagcc      240

ggcatttcaa gagaagacct gtttgttaca tcaaaggtct ggaatgacga tttaggctat      300

gacgaaacga ttgcagccta tgaggcgagt ctcgaaaagc tcggacttga ctaccttgat      360

ttatacctga tccactggcc tgttgaagga cgctacaaag cggcgtggaa agcgcttgaa      420

acactttatg aacaaggacg cgtaaaagca atcggagtga gcaattttca gattcaccat      480

ctggaagact tgctgaaaga tgccgccgtc aaaccggcga tcaaccaggt tgagtatcat      540

ccgcggctga cgcagaaaga gctgcaagcg ttttgccgtg cgcacggcat ccagctgcaa      600

gcatggtcgc cgctgatgca aggccaattg ctcagccatc cactgctgaa agatatcgcg      660

gacaagtacg gcaagacacc ggcccaagtc attttgcgct gggatttgca aaacggggtc      720

gttacgattc cgaagtcgac taaagcggag cggattgccc aaaacgcgga catatttgat      780

tttgaactga ccaccgagga aatgaagcaa attgacgcgc tgaatgaaaa cacccgtgtc      840

ggccctgatc ccgataactt tgacttttaa caaaacggcc ccgttcgaca ttcgaacggg      900

gctttaattg aattgtgcgg ttacaccgcc ggactccatc atcatcagtt cttttttcat      960

atccaatccg ccccggtatc ccgtgagctg cccgctttta ccgataaccc gatggcaagg     1020

caccaccatt aacagcggat ttgcgccgat cgccgcgcct actgcccgca cagcggcctg     1080

cttttcaata tgctcggcga tatcggaata ggagcaagtg ctgccgtaag ggatttcgga     1140

gagcgccttc cacactgcca gctgaaaagg cgtgccggca aggtcgacag gaaagctgaa     1200

atgagttcgc ttgccgttca aatacgcctg cagctgctcg gcgtattctg ccaatccttt     1260

gtcatcccga atgaaaactg gctgtgtaaa tcttttttca gcccaagcgg ccaaatcctc     1320

gaagccttga ttccatcccc ctgtaaaaca gagcccgcgg gcagtcgccc caatgtgaat     1380

ctgccaacct cggcaaataa gcgtacgcca gtatacgatt tgatcgtcca tatgtttacc     1440

tccgtttcat ttgccggtac gacgtcggcg attgcccagt cttcttttta aacaaagagg     1500

caaaatattc cgcattcgca atgcctacca ttgaagcgat ttctgcgatc gatcgttctg     1560

aatgagcaag caaatcgacc gctttctcaa tccttttctg caggatgtat tctgccggcg     1620

agacgccttt gattcgttta aatgtccgct gcaggtgaaa agggctgata tggcacctgt     1680

cagccaaagc ttgcagagac agcggatcgc gataagattc ctcgatgatt tccaccacac     1740

gctgtgccag ctcttcatcc ggcagcagcg ccccggccgg attgcagcgt ttgcaggggc     1800

ggtacccttc tgataaagca tcttttgcat tgaaaaagat ctgcacattg tcgatttgcg     1860

gaactctcga tttgcaggaa gggcggcaaa atatgccggt cgttttgacc gcgtaataaa     1920

aaactccgtc ataggcggaa tcgttttccg taatcgcccg ccacatttca ggcgtcaatc     1980

gtgatttgct gttcatatct tcaccccgat ctatgtcagt ataacctata tgacagccgg     2040

aggtggagag gcggagaacg gcacagcaag aagacaaaga agaagagaga ctgttgcctg     2100

gacctccgaa acgcgctaca attcatttac aacacaggat ggggtgagaa tattgccgga     2160

atcagtgaag caggcctcct aaaataaaaa tctatatttt aggaggtaaa acatgaattt     2220

tcaaacaatc gagcttgaca catggtatag aaaatcttat tttgaccatt acatgaagga     2280

agcgaaatgt tctttcagca tcacggcaaa cgtcaatgtg acaaatttgc tcgccgtgct     2340

caagaaaaag aagctcaagc tgtatccggc ttttatttat atcgtatcaa gggtcattca     2400

ttcgcgccct gagtttagaa caacgtttga tgacaaagga cagctgggtt attgggaaca     2460

aatgcatccg tgctatgcga tttttcatca ggacgaccaa acgttttccg ccctctggac     2520

ggaatactca gacgattttt cgcagtttta tcatcaatat cttctggacg ccgagcgctt     2580

tggagacaaa aggggccttt gggctaagcc ggacatcccg cccaatacgt tttcagtttc     2640

ttctattcca tgggtgcgct tttcaaactt caatttaaac cttgataaca gcgaacactt     2700

gctgccgatt attacaaacg ggaaatactt ttcagaaggc agggaaacat ttttgcccgt     2760

ttccttgcaa gttcaccatg cagtgtgtga cggctatcat gccggcgctt ttataaacga     2820

gttggaacgg cttgccgccg attgtgagga gtggcttgtg tgacagagga aaggccgata     2880

tgattcggcc ttttttatat gtacttctta gcgggtctct aaaataacca aaaagcaagg     2940

actgctgaaa gggctgacat aagccttttg ccggcggtcc ttttttaatt ctgatttttc     3000

aaacttagcc ccctcgagca cacaaggacg cggtacgttc acaatggtct ttgaccacta     3060

tgaagaagtg cctaagagca tcgctgatga gatcatcaaa aaaaatcaag gtgaataatt     3120

gattttgcct cttcattaaa gtataactac tattacagaa tagtctttta agtaagtcta     3180

ctctgaattt ttttaaaagg agagggtaaa gattgaataa atcagtcata ccttctttaa     3240

ttacggttgg aaactttgtt tcgggcatct cttcaatact gcttgcattc agagggtatt     3300

tattcttagc cattatattt gttctgatcg gcgctatctt ggacagcctt gacggcatgg     3360

ccgcgcgcag attaaatgcc gtcagcccgt ttggaaaaga attagattct ttgtctgata     3420

ttatcacttt cggagtcgcc ccggccatca tcacctattc aatcgttttt tatgatgctc     3480

cgatcttagg ccttccttcg acattgttat tccctgtttg cggagcgctt cggctggcgc     3540

gatttaatat tcaatcagag aaccaggatt attttacagg ccttccgatc accgcagccg     3600

gaacgattct ggtatgctta aaccttttct cagaaatcct gggcaaaaag ccgttcattt     3660

tgatcatgct gttcctgtcc tttctcatgg tcagcaaaat caaagtcatc agcctgaagg     3720

ggaaaaaact attgaagaaa gtgagctaga agagcagaga ggacggattt cctgaaggaa     3780

atccgttttt ttatttttaa catctctcac tgctgtgtga ttttactcac ggcatttgga     3840

acgccggctc tcaacaaact ttctgtagtg aaaatcatga accaaacgga tcgtcggcct     3900

gattaacagc tgaaagctgc cgatcacaaa catccatagt cccgccggct tcagttcctc     3960

ggagaaaaag cagaagctcc cgacaaggaa taaaaggccg atgagaaaat cgtttaatgt     4020

atgtagaact ttgtatcttt ttttgaaaaa gagttcatat cgattgttat tgttttgcgg     4080

cattgcttga tcactccaat ccttttattt accctgccgg aagccggagt gaaacgccgg     4140

tatacatagg atttatgaat taggaaaaca tatggggaaa taaaccatcc aggagtgaaa     4200

aatatgcggt tattcatatg tgcatcgtgc ctgttcggct tgattgttcc gtcatttgaa     4260

acgaaagcgc tgacgtttga agaattgccg gttaaacaag cttcaaaaca atgggaagtt     4320

caaatcggta aagccgaagc cggaaacgga atggcgaaac cggaaaaagg agcgtttcat     4380

acttatgctg tcgaaatcaa aaacattgga cacgatgtgg cttcggcgga aatttttgtc     4440

tatcggaacg agcctaattc ttcaacgaaa ttttcgcttt ggaacattcc tcacgaaaat     4500

ccggtttctt tagccaaaag cttaaatcac ggaagctctg tcaagcaccg caatctgctt     4560

atggcagaga atgcgaccga attggaagtg gacatgattt ggacggaaaa aggaagcgaa     4620

ggcagacttt taaaggaaac gttcattttc aagggagatg aatcatgaag aaaaaatggc     4680

cgttcatcgt caacggtctt tttttaatga cttaggcagc cgatcgttcg gccatacgat     4740

atcgaagcga cctcgaacca gcagagctcg tcacaaaaca tttgcattta aagaaaaata     4800

caggatgttt tcaccaatat ttttctcaat gatgatacac tattgacaag ctgctacttt     4860

gggagggtgt ttccatagat gccgatgaag caaaaacacc aaatgtgtca tgagagctct     4920

ctctaatcga tataaaagta gggtgaaccg gggttgtcaa tctgtaaaag atcttttttt     4980

atcccgtgat acgcttttgg aattctgaat cttcaagaaa gtccccagcc ttttgctgat     5040

caatcgagaa caaaggatga tacatatgaa aagaatagat aaaatctacc atcagctgct     5100

ggataatttt cgcgaaaaga atatcaatca gcttttaaag atacaaggga attcggctaa     5160

agaaatcgcc gggcagctgc aaatggagcg ttccaatgtc agctttgaat taaacaatct     5220

cgttcgggcc aaaaaggtga tcaagattaa aacgttcccc gtccgctaca tcccggtgga     5280

aattgttgaa aacgtcttga acatcaaatg gaattcagag ttgatggagg ttgaagaact     5340

gaggcggctg gctgacggcc aaaaaaagcc ggcgcgcaat atatccgccg atcccctcga     5400

gctcatgatc ggggctaaag ggagcttgaa aaaggcaatt tctcaggcga aagcggcagt     5460

cttttatcct ccgcacggct tgcatatgct gctgctcggg ccgacgggtt cggggaaatc     5520

gctgtttgcg aatcggatct accagttcgc cgtttattct gacatattga agcccgattc     5580

cccgttcatc acattcaact gtgcagatta ctataacaac cctcaattat tgctctctca     5640

attgttcgga cataaaaaag ggtcttttac aggtgcgggt gaagacaaag caggattagt     5700

cgagcaggcg gacgggggca ttctgtttat ggatgaaatc catcgcctcc cgccggaggg     5760

gcaggaaatg ctgttttatt tcatagacag cggcacatac aacaggcttg gtgaaacaga     5820

gc                                                                    5822


<210>  13
<211>  3308
<212>  DNA
<213>  Bacillus licheniformis

<400>  13
atgagcaaca ctgaacttga tttgctgagg cagcaggcga atgaattaaa cttgcaaata       60

ttaaagctga ttaacgagcg aggaagaatc gttcaggaga ttggaaaagc gaaggaagca      120

caaggcatca accgctacga tcctgtcaga gaaagagcga tgctgaacga aattattgaa      180

aataacgacg gaccgtttga aaactcgacg atccagcata tcttcaaaga gattttcaaa      240

gccgggcttg aactgcagga agacgatcac agcaaagcgc tgctcgtttc tcgcaagaaa      300

aagcctgaaa atacaatcgt tgatctgaaa ggcgaaaaaa tcggcgacgg agaacaaaga      360

tttatcgtcg gtccgtgtgc ggttgaaagc tacgaacaag tagcggaagt cgcagcggca      420

gctaaaaagc aaggcttgaa actgcttcgc ggcggagctt tcaaaccgcg tacaagcccg      480

tacgacttcc aaggcctcgg cgtggaaggc ctgcaaatct taaagcgtgt tgctgatgag      540

tatgatctgg ccgtcatcag tgagatcgtg aatccgcagc atattgaaga agccattgat      600

tacatcgatg tcatccaaat cggcgcccgc aacatgcaga acttcgagct cttaaaagcg      660

gccggttcag tgaagaagcc ggttctgctg aagcgcggac ttgccgcaac gctgaaggaa      720

ttcatcaatg cagcggagta catcatgtcg cagggcaatg atcaaatcat cctttgtgaa      780

cgcggaatca gaacgtatga aacagcgact agaaatacgc ttgatatctc agctgtgccg      840

atcctgaagc aggaaactca ccttcctgta ttcgtggatg tcactcattc aaccggacgc      900

cgcgacctgc ttcttccgac agcaaaagca gcgcttgcga tcggagctga cggtgtcatg      960

gccgaggtcc atcctgatcc ttctgtcgcg ctttccgact cagctcagca aatggatatc     1020

ccgacatttg aaaaatggtt aaatgaactg aagccgctcg ttcaagtaaa agcataagat     1080

ttgtttatgg accgctcgcg agcggtcctt tttttacgtt gtcatcaaaa attgtcgaaa     1140

aaatttctta tttttaagta aaaagctgtt tattcttatt taaattaatt ataaaaataa     1200

agggaacgtt ttcatattag gtaaaaccgt gtataatttc agagagcccc ttttgttttg     1260

gcaggttatg aaaaataatg taaactggtg ttctgtttat tttacataaa gttttacatt     1320

ttgacatttc tctacatgaa aatgtttatg ctataggaaa agaaaagtgt acccagttaa     1380

ggagtggtaa aaatgagtaa tgtgacaata tatgatgtag cacgcgaagc aaatgtaagt     1440

atggcaaccg tttcccgggt cgtcaacggg aatccgaatg tcaagccgac gacgagaaag     1500

aaggtgcttg aagcgatcga gcgtcttggc tatcgtccaa acgccgtggc aagagggctg     1560

gcaagcaaaa agacgacgac tgtcggcgtg atcattcccg atatttccag tatcttttat     1620

tcagagctgg cgaggggaat cgaagatatc gcaacgatgt acaagtacaa tattattttg     1680

agcaattccg accagaatat ggacaaagaa cttcatcttt tgaatacgat gctaggaaag     1740

caagttgacg gtatcgtctt tatgagcgga aatgtgactg aagagcatgt ggaggaattt     1800

aagcgatcgc cagttccgat cgtgcttgcg gcatctgttg aagaaaaagg ggaaacgccg     1860

tcggttgcga tcgattatga acaggcgatt tatgatgcgg caaccatgct gattgagaaa     1920

ggccataagc gccttgcgtt cgtctcagga cctatggctg aaccggttaa tcaagcgaaa     1980

aaactgcaag gctttaaaag agcgcttggg gataaggggc tgcaatttaa ggaagagtat     2040

gttgtagaag gcgattatac gtacgattca ggaatggaag cgctggaggc tttaatgaag     2100

ctggatgaaa aaccgacagc cgtcctgtca gcgacagacg aaatggcgct cggcgttatt     2160

cacgcagcac aggataaagg actggctgtt ccggatgacc ttgaagtgat cggttttgac     2220

aatacaaggc tttcattaat ggttcggccg cagctgtcga ctgtcgtcca gcctacgtat     2280

gatatcggtg ccgtggcgat gaggcttctg acaaagctga tgaacaaaga ggaagtcgaa     2340

gagcatattg tccagctgcc gcatcgcatt gaactcagac aatcaacaaa atgatcatca     2400

ttaaataaac gagaaagaaa gcaagtgttc acagcttttt tcgtgaattg cttgctttct     2460

tttccttgag ctggagagat tatgaaaaaa tgaaacgatt tgattatctg acaccggtcg     2520

gttttattct gggtattttc attttagtgc tcggcgttgt ctctggagcg gggctttccg     2580

gtttttattc gtttatcgat ttgacgtctt tttttattgt gacgggtgga ttgtgtgcag     2640

ctgtattcat cagcttttcc ccgaaagatt taaaaagagc gccggctgtg ctgaagcagg     2700

tctttatttc ggaggaagat gatgtgcgcg aattggtcaa aacctttgtc agcctgtctg     2760

aacaggcgcg caaacaagga attttattat tggacgccaa tgtcaatgaa atgaaagacc     2820

cgtttttgaa aaaaggcctg ctccttgcca tcgacggatg ggatgaggag acgatccgca     2880

atgtgatgaa ctcggaaatt gccgcgatgg aagaacgaca cagaaagggg agaaggatct     2940

ttgaaaaagc aggagaattt gcgcctgcat ggggaatgat cggaacgctt gtcggtctcg     3000

tcatgatgct caaaaacctg aacaagccgg aaacgctcgg tccgaatatg gcaatcgccc     3060

ttttgacgac gctttacgga tcacttttag ccaacatgct gtttataccg atcgccgcga     3120

agcttgaaga aaagacggag aatgaaatct ttaagaagca agtcatgatc gaaggcatca     3180

tcggcatcca gtcgggaaga aatccgcgaa atcttgaaag tcagcttgtc gttttcagtt     3240

caaaagaaga atggacaaaa aagcgggcgg accgcatgaa acaaaaggac aggtctcatg     3300

aagcttag                                                              3308


<210>  14
<211>  924
<212>  DNA
<213>  Bacillus licheniformis

<400>  14
atcttcaccc cgatctatgt cagtataacc tatatgacag ccggaggtgg agaggcggag       60

aacggcacag caagaagaca aagaagaaga gagactgttg cctggacctc cgaaacgcgc      120

tacaattcat ttacaacaca ggatggggtg agaatattgc cggaatcagt gaagcaggcc      180

tcctaaaata aaaatctata ttttaggagg taaaacatga attttcaaac aatcgagctt      240

gacacatggt atagaaaatc ttattttgac cattacatga aggaagcgaa atgttctttc      300

agcatcacgg caaacgtcaa tgtgacaaat ttgctcgccg tgctcaagaa aaagaagctc      360

aagctgtatc cggcttttat ttatatcgta tcaagggtca ttcattcgcg ccctgagttt      420

agaacaacgt ttgatgacaa aggacagctg ggttattggg aacaaatgca tccgtgctat      480

gcgatttttc atcaggacga ccaaacgttt tccgccctct ggacggaata ctcagacgat      540

ttttcgcagt tttatcatca atatcttctg gacgccgagc gctttggaga caaaaggggc      600

ctttgggcta agccggacat cccgcccaat acgttttcag tttcttctat tccatgggtg      660

cgcttttcaa acttcaattt aaaccttgat aacagcgaac acttgctgcc gattattaca      720

aacgggaaat acttttcaga aggcagggaa acatttttgc ccgtttcctt gcaagttcac      780

catgcagtgt gtgacggcta tcatgccggc gcttttataa acgagttgga acggcttgcc      840

gccgattgtg aggagtggct tgtgtgacag aggaaaggcc gatatgattc ggcctttttt      900

atatgtactt cttagcgggt ctct                                             924


<210>  15
<211>  98
<212>  DNA
<213>  Bacillus subtilis

<400>  15
aaaataacca aaaagcaagg actgctgaaa gggctgacat aagccttttg ccggcggtcc       60

ttttttaatt ctgatttttc aaacttagcc ccctcgag                               98


<210>  16
<211>  537
<212>  DNA
<213>  Bacillus licheniformis

<400>  16
ttgaataaat cagtcatacc ttctttaatt acggttggaa actttgtttc gggcatctct       60

tcaatactgc ttgcattcag agggtattta ttcttagcca ttatatttgt tctgatcggc      120

gctatcttgg acagccttga cggcatggcc gcgcgcagat taaatgccgt cagcccgttt      180

ggaaaagaat tagattcttt gtctgatatt atcactttcg gagtcgcccc ggccatcatc      240

acctattcaa tcgtttttta tgatgctccg atcttaggcc ttccttcgac attgttattc      300

cctgtttgcg gagcgcttcg gctggcgcga tttaatattc aatcagagaa ccaggattat      360

tttacaggcc ttccgatcac cgcagccgga acgattctgg tatgcttaaa ccttttctca      420

gaaatcctgg gcaaaaagcc gttcattttg atcatgctgt tcctgtcctt tctcatggtc      480

agcaaaatca aagtcatcag cctgaagggg aaaaaactat tgaagaaagt gagctag         537


<210>  17
<211>  178
<212>  PRT
<213>  Bacillus licheniformis

<400>  17

Leu Asn Lys Ser Val Ile Pro Ser Leu Ile Thr Val Gly Asn Phe Val 
1               5                   10                  15      


Ser Gly Ile Ser Ser Ile Leu Leu Ala Phe Arg Gly Tyr Leu Phe Leu 
            20                  25                  30          


Ala Ile Ile Phe Val Leu Ile Gly Ala Ile Leu Asp Ser Leu Asp Gly 
        35                  40                  45              


Met Ala Ala Arg Arg Leu Asn Ala Val Ser Pro Phe Gly Lys Glu Leu 
    50                  55                  60                  


Asp Ser Leu Ser Asp Ile Ile Thr Phe Gly Val Ala Pro Ala Ile Ile 
65                  70                  75                  80  


Thr Tyr Ser Ile Val Phe Tyr Asp Ala Pro Ile Leu Gly Leu Pro Ser 
                85                  90                  95      


Thr Leu Leu Phe Pro Val Cys Gly Ala Leu Arg Leu Ala Arg Phe Asn 
            100                 105                 110         


Ile Gln Ser Glu Asn Gln Asp Tyr Phe Thr Gly Leu Pro Ile Thr Ala 
        115                 120                 125             


Ala Gly Thr Ile Leu Val Cys Leu Asn Leu Phe Ser Glu Ile Leu Gly 
    130                 135                 140                 


Lys Lys Pro Phe Ile Leu Ile Met Leu Phe Leu Ser Phe Leu Met Val 
145                 150                 155                 160 


Ser Lys Ile Lys Val Ile Ser Leu Lys Gly Lys Lys Leu Leu Lys Lys 
                165                 170                 175     


Val Ser 
        


<210>  18
<211>  136
<212>  DNA
<213>  Bacillus licheniformis

<400>  18
cacacaagga cgcggtacgt tcacaatggt ctttgaccac tatgaagaag tgcctaagag       60

catcgctgat gagatcatca aaaaaaatca aggtgaataa ttgattttgc ctcttcatta      120

aagtataact actatt                                                      136


<210>  19
<211>  227
<212>  DNA
<213>  Bacillus licheniformis

<400>  19
aaatgtgcgc ggtccccctg tttcaggggg ttttttagtt tgaaaaaaac tttcgctact       60

aaaaaaattt cggccccgta tttttttaaa aaaacataat tgaatataaa taatacagtt      120

aaaactacta agaatctgta aatagaagta agctgggcca aaaaataaga aaaaaaaact      180

atagttaaac atttaacaaa tgtctgataa ttgtttataa tataaat                    227


<210>  20
<211>  430
<212>  PRT
<213>  Pseudomonas saccharophila

<400>  20

Ala Asp Gln Ala Gly Lys Ser Pro Ala Gly Val Arg Tyr His Gly Gly 
1               5                   10                  15      


Asp Glu Ile Ile Leu Gln Gly Phe His Trp Asn Val Val Arg Glu Ala 
            20                  25                  30          


Pro Tyr Asn Trp Tyr Asn Ile Leu Arg Gln Gln Ala Ser Thr Ile Ala 
        35                  40                  45              


Ala Asp Gly Phe Ser Ala Ile Trp Met Pro Val Pro Trp Arg Asp Phe 
    50                  55                  60                  


Ser Ser Trp Thr Asp Gly Asp Lys Ser Gly Gly Gly Glu Gly Tyr Phe 
65                  70                  75                  80  


Trp His Asp Phe Asn Lys Asn Gly Arg Tyr Gly Ser Asp Ala Gln Leu 
                85                  90                  95      


Arg Gln Ala Ala Gly Ala Leu Gly Gly Ala Gly Val Lys Val Leu Tyr 
            100                 105                 110         


Asp Val Val Pro Asn His Met Asn Arg Phe Tyr Pro Asp Lys Glu Ile 
        115                 120                 125             


Asn Leu Pro Ala Gly Gln Arg Phe Trp Arg Asn Asp Cys Pro Asp Pro 
    130                 135                 140                 


Gly Asn Gly Pro Asn Asp Cys Asp Asp Gly Asp Arg Phe Leu Gly Gly 
145                 150                 155                 160 


Glu Ala Asp Leu Asn Thr Gly His Pro Gln Ile Tyr Gly Met Phe Arg 
                165                 170                 175     


Asp Glu Phe Thr Asn Leu Arg Ser Gly Tyr Gly Ala Gly Gly Phe Arg 
            180                 185                 190         


Phe Asp Phe Val Arg Gly Tyr Ala Pro Glu Arg Val Asp Ser Trp Met 
        195                 200                 205             


Ser Asp Ser Ala Asp Ser Ser Phe Cys Val Gly Glu Leu Trp Lys Glu 
    210                 215                 220                 


Pro Ser Glu Tyr Pro Pro Trp Asp Trp Arg Asn Thr Ala Ser Trp Gln 
225                 230                 235                 240 


Gln Ile Ile Lys Asp Trp Ser Asp Arg Ala Lys Cys Pro Val Phe Asp 
                245                 250                 255     


Phe Ala Leu Lys Glu Arg Met Gln Asn Gly Ser Val Ala Asp Trp Lys 
            260                 265                 270         


His Gly Leu Asn Gly Asn Pro Asp Pro Arg Trp Arg Glu Val Ala Val 
        275                 280                 285             


Thr Phe Val Asp Asn His Asp Thr Gly Tyr Ser Pro Gly Gln Asn Gly 
    290                 295                 300                 


Gly Gln His Lys Trp Pro Leu Gln Asp Gly Leu Ile Arg Gln Ala Tyr 
305                 310                 315                 320 


Ala Tyr Ile Leu Thr Ser Pro Gly Thr Pro Val Val Tyr Trp Pro His 
                325                 330                 335     


Met Tyr Asp Trp Gly Tyr Gly Asp Phe Ile Arg Gln Leu Ile Gln Val 
            340                 345                 350         


Arg Arg Thr Ala Gly Val Arg Ala Asp Ser Ala Ile Ser Phe His Ser 
        355                 360                 365             


Gly Tyr Ser Gly Leu Val Ala Thr Val Ser Gly Ser Gln Gln Thr Leu 
    370                 375                 380                 


Val Val Ala Leu Asn Ser Asp Leu Ala Asn Pro Gly Gln Val Ala Ser 
385                 390                 395                 400 


Gly Ser Phe Ser Glu Ala Val Asn Ala Ser Asn Gly Gln Val Arg Val 
                405                 410                 415     


Trp Arg Ser Gly Ser Gly Asp Gly Gly Gly Asn Asp Gly Gly 
            420                 425                 430 


<210>  21
<211>  9175
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette

<400>  21
ctagcgtgtc ctcgcatagt tcttagattg tcgctacggc atatacgatc cgtgagacgt       60

catcggacag ctcttgcttg atatcttcaa aatgacgccg gctcatgtca tgtcaacttt      120

tgtcgtatct ggagcgatcc ttgacggatt cggcatttac gaccgtttta tcgaatttgc      180

cggtgccggg gctacagtcc cgattgtcag cttcggccac tctcttttgc acggcgcgat      240

gcaccaggct gagaaacatg gctttatcgg aatcggcatg gggatatttg aactgacatc      300

tgccggtata tctgccgcta tcttgttcgc ttttcttgtt gccgtgattt ttaaaccgaa      360

aggataaagg aaaatgccag caaaacgcaa ggtcattttg gtcacagacg gcgatatata      420

cgctgcaaaa gcaatcgaat atgcagcaag aaaaacgggt ggccgctgca tttcccaatc      480

ggcggggaat ccgagcgtta aaacaggacc ggagcttgta accatgatcc tgcaaacccc      540

tcatgatcct gtattcgtca tgtttgatga ttccggactt caaggtgaag gcccgggaga      600

gacagctatg aaatatgtag cgatgcatcc cgatatcgag gtgctcggag tcatcgccgt      660

cgcttcaaaa actcattatg cagagtggac gagagtcgat gtatcaatcg atgcagaagg      720

cgaactgaca gagtacggcg tcgataaaca cggggtcaaa gagttcgatg tcaaacgaat      780

gaatggtgat acagtctatt gccttgacca gctggatgtt ccgatcattg tcggaatcgg      840

tgatatcggt aagatgaaca gaaaagacga tgtggaaaaa ggttcgccga ttacaatgaa      900

agcggtcgag ctcattttag aaaggagcgg gtatcatgag tgctcaaaag caagagaaga      960

cgaacgtatt ccttgatcct tctaagaatg aagcgtattt caagaagcgg gtcggcatgg     1020

gagaaagctt tgaccttggc gtacggaagg tctttattct cggacatgaa gttcagcttt     1080

attatgtcaa cggattgtgc gacacacaat acatcattca cctgttaaga gaactggtgc     1140

atctgaatga taaagaaaaa gaatcgggcg aggtcgaaga catcgtcgaa aacaggcttt     1200

tgaaccagca ggtttcaaaa gcggaaacgc ttgatgaagc tgtcgaccaa gtgttgtcag     1260

gactggttgc catcatcgtc gaagatgcgg gctttgcttt tatcatcgat gtcagaagct     1320

acccgggcag aacgccggaa gaacctgata cagaaaaagt cgtacgcggt gcaagggacg     1380

gactcgtcga gaacatcatc gtcaacacag ccctgattag acgccggatc agagatgagc     1440

gcttgcgcta caaaatgctt catatcggtg aacgctctaa aacagacatc tgcctctgct     1500

atttggaaga cgttgcagat cccgatcttg ttgaagtatt aaaaaaagaa attgaagatg     1560

tgaagatcga cgggctgccg atgtcggata aatcggtaga ggaattcctg gtcggccaag     1620

gctacaatcc gtttccgctt gtcaggttta cggaaagggc agacgtagcc gcaagccata     1680

ttttagaggg gcatgtcatc gtgatcgtcg atacgtcgcc aagcgtcatc atcacaccga     1740

ccactttgtt tcaccatgtt cagcatgctg aggaatacag acagacgccg gctgttggga     1800

cgtttttaag gtgggtgcgg tttttcggta ttttggcctc cacctttttg ctgccgcttt     1860

ggctgctgtt tgtcattcat ccgtcgctct tgcctgataa tttatcgttt atcgggttga     1920

ataaagacac ccatattccg attatcatgc agattttcct ggcggatctc ggcgtcgaat     1980

ttttaagaat ggccgccatt catacgccga cggcgctttc gactgcaatg ggcctgatcg     2040

ccgctgtatt gatcggcgat atcgcgatca atgtcggctt gttttctccc gaagtcattt     2100

tatacgtttc cctctcggca atcggagcct acacgacacc aagctacgag ctgagcctgg     2160

cgaataaaat ggtgaagctg tttatgctga tattggtggc gctttttaaa gtggagggat     2220

ttgtcatcgg attaacgatc ttaactatag tgatgacttc gatcaggtca ttgcgaacgc     2280

cttacttatg gcctctcctc ccgttcaatg gaaaagcgtt ttggcatgtt ctcgtgcgca     2340

cgtccgttcc agggggaaaa gtcaggccga gcatcgttca tccgagaaac cgctccagac     2400

agccgtgaag ccggcattcg aagaggcttt tccccgggga aaagcctctt tttcaataat     2460

cgaattccgg tctttgagta ccgatgcctc tgtattcatt ggcagagatc gcgactgccc     2520

ggaggctgca gatgttgttc tgtcttctga tcggatagac gacatacagc atttcgcggc     2580

cgtacgggtc aatcgttgac gaatgaagga aaacctcagt tcctctccgc caaaatctcg     2640

tattcgccgg agctgtaata atctgccctt cataaggctc ataaattctc tgttcataat     2700

gcgcagccgg ctgataaggg gcgtatacat cttcaggtgc atagccggga gcgggggtgt     2760

agggatagcg atttggatac atatgataac ctctttccca cttcgttttt tggttttcat     2820

ctttaagatt atattcaggt aaatgcctat ttgtatgggc gaaaatctca gcttttcggc     2880

tcttttttta ttgaatggac gttgtgtatg cctatttcta tcaagcgctg ttttctgtta     2940

ttctataatc aatagaatgg attagttgtt tagggaatca tttcctttat aaatcaagaa     3000

aatttggaca aatggtggtt tagtttttaa aacgaaatgt tataatacaa cataagaatc     3060

gcactatcat gaagccggaa gatgcatcgg gcagcaaccg gagcgcccct tgcacctttg     3120

tcgatagaga aagagggaat gacaattgtt tttacacggt actagcagac aaaatgaaag     3180

agggcacctc gaaatcggcg gtgtcgatgt tctatcattg gcagaaagat acggaacacc     3240

tctttatgta tacgatgtcg cgctgattag agagcgcgcc cgaaaattcc agaaggcatt     3300

caaggaagcc ggtttaaaag cgcaggtagc gtatgcaagc aaggcgtttt catcggttgc     3360

catgattcag cttgccgaac aagaggggct gtctctggat gtggtatcgg gaggagagct     3420

tttcactgcg atcaaagcag ggttcccagc tgagcggatt cattttcacg gaaacaataa     3480

gagccctgaa gaactagcca tggcgctgga gcatcaaatc ggctgcatcg tgctcgataa     3540

ctttcacgag atcgccatta cagaagatct ttgcaagcga tcaggacaaa ctgtagacgt     3600

tttgctcaga atcactccgg gagttgaagc gcacacgcac gattatatta cgacggggca     3660

ggaagattcc aaattcggtt ttgatctgca taatggacag gtcgaacaag ccatcgaaca     3720

agtcctccgc tcgtctgcgt ttaagctcct cggcgtgcac tgccacatcg gttcgcaaat     3780

ttttgatacg gcaggatttg tccttgcagc agacaagatt ttcgagaagc ttgcggaatg     3840

gcgggagact tactctttca ttccggaagt gctcaatctt ggcgggggct tcggcatccg     3900

ctatacaaaa gacgacgagc cgcttgcagc tgatgtttat gttgaaaaaa tcatcgaggc     3960

ggtcaaagca aatgccgagc atttcggctt tgacatccct gagatttgga tcgaaccagg     4020

ccggtctctc gtcggtgatg cggggactac gctgtacacg atcggttctc aaaaagaggt     4080

gccgggcatt cgcaaatatg tagccatcga cggcggcatg agcgataata tcaggccggc     4140

gctttatgag gcaaaatatg aagcagccgt cgccaacagg atgaacgatg cttgtcatga     4200

taccgcatca atcgcaggaa aatgctgcga aagcggagat atgctgattt gggatttgga     4260

aatccccgaa gttcgcgacg gagatgtgct cgccgttttc tgcaccggtg cgtacggcta     4320

cagcatggcc aacaactaca accgcattcc gcgcccggcc gtcgtctttg tcgaggacgg     4380

ggaagcgcag ctcgtcattc agagagagac gtatgaggat atcgtcaagc tggatctgcc     4440

gctgaaatcg aaagtcaaac aataaaaaaa tggagattcc ctaagagggg ggtctccatt     4500

tttaattcag agctcgtcgc tgataaacag ctgacatcaa tatcctattt tttcaaaaaa     4560

tattttaaaa agttgttgac ttaaaagaag ctaaatgtta tagtaataaa acagaatagt     4620

cttttaagta agtctactct gaattttttt aaaaggagag ggtaaagaat gaaacaacaa     4680

aaacggcttt acgcccgatt gctgacgctg ttatttgcgc tcatcttctt gctgcctcat     4740

tctgcagctt cagcagctga tcaagcagga aaaagcccgg caggcgtcag atatcatggc     4800

ggtgatgaaa tcatccttca gggctttcat tggaacgtcg tcagagaagc gccgtataac     4860

tggtataaca tcctgagaca acaagcgagc acaattgccg ctgatggctt ttccgcaatc     4920

tggatgccgg ttccgtggag agattttagc agctggacgg atggagataa aagcggaggc     4980

ggcgaaggat atttttggca tgactttaac aaaaacggcc gctatggaag cgatgctcaa     5040

ctgagacaag cagcaggagc acttggagga gcaggagtca aagtcctgta cgatgtcgtc     5100

ccgaaccata tgaaccgctt ttatccggac aaagaaatca atctgccggc aggccaaaga     5160

ttttggagaa acgattgccc ggacccggga aatggaccga atgattgcga tgatggcgat     5220

agatttctgg gcggcgaagc ggatctgaat acaggccatc cgcaaatcta tggcatgttt     5280

cgggacgaat ttacgaatct gagaagcgga tatggagcgg gcggatttcg ctttgatttt     5340

gtcagaggct atgccccgga aagagttgat agctggatga gcgattcagc ggatagcagc     5400

ttttgcgtcg gcgaactttg gaaagaaccg agcgaatatc cgccgtggga ttggagaaat     5460

acagcgagct ggcagcagat catcaaagat tggagcgata gagcaaaatg cccggtcttt     5520

gactttgccc tgaaagaacg catgcaaaat ggaagcgtcg ccgattggaa acatggcctg     5580

aacggaaatc cggacccgag atggagagaa gtcgccgtca cgtttgtcga taaccatgac     5640

acaggatata gcccgggaca aaatggagga caacataaat ggccgcttca agatggcctt     5700

atcagacagg cgtatgccta tatccttaca tcaccgggaa caccggttgt ttattggccg     5760

catatgtatg attggggcta tggcgatttc atccgccaac tgatccaggt tagaagaaca     5820

gcaggagtca gagcggatag cgccattagc tttcatagcg gctatagcgg acttgtcgct     5880

acagttagcg gcagccaaca aacactggtc gtcgccctga atagcgatct ggcaaatccg     5940

ggacaagttg ctagcggcag ctttagcgaa gcagtcaatg ccagcaatgg ccaagtcaga     6000

gtctggagaa gcggaagcgg agatggagga ggaaatgacg gcggctaaaa gagcagagag     6060

gacggatttc ctgaaggaaa tccgtttttt tattttcaag cacgaaaaac acttcccggt     6120

gatcgggagg tgttttttgt taaaaagatc atgacatgca tagaacagcg accgggctaa     6180

ttgtatataa tattgtgaat ttaacaaaaa atttacaaag gagatgataa aggcaatgac     6240

cagggtgaaa aggatgagat ttgctgattt gttggattta gaggcggagt agatgaaacc     6300

ggccaaagta tccctactcc accgattgct ccagtgcctg aagcaatgtg ttgattgtaa     6360

cacagtaaat cgttttacag caataaacat ttttgtgaat attttattga tttcggctgt     6420

gatctcattc ccatattctg ctgcggccca tggcgcaaca cagtccggcg atcaatattc     6480

aagctttgaa gaattggagc ggaatgaaga tccagcttct taccgaatta cggagaagaa     6540

cgcaagagtg ccgatgctca tcatggccat ccatggaggc ggcatcgaac ccggaacgag     6600

cgaaatcgcc aatgaagtgt ccaaaaacta ttccctgtac ttgtttgaag ggctgaaatc     6660

atcaggcaat acggaccttc acattacaag cacgcgtttt gacgagccag cggcgctcgc     6720

aattactgca agccaccagt atgtcatgtc gctccacggc tattacagtg aagaccgcga     6780

tattaaagta ggcggcacag accgcgctaa aatcagaata ttggttgatg agctgaaccg     6840

ctcggggttt gccgctgaaa tgctggggac agatgacaag tatgccggaa cccatccgaa     6900

taacatcgcc aacaagtcgc tttccgggct gagcattcag cttgaaatga gcacgggttt     6960

ccgcaaatct ttattcgacc ggtttacact aaaagacagg gcggcgacgc aaaacgaaac     7020

gttttaccga tttacaaagc tgctgacaga ttttattcat gaaaactatg aagaagacgg     7080

aggggatttc ccctctgcaa aaataaaaca cccccttcaa gtgaaaaagg aggtgtttcg     7140

gcggttgtgt taaccgttgg actctgaggt gccgccgccg gtgaatacgg aaacgatggc     7200

gttccacaga gacacaaaga agtcgatcag tttttgaaga aagttttgtc cttcttcaga     7260

atccaagaat ttcgtgattt tatcctttgc tttgtcaagc tggtctccaa cctggttcca     7320

gtcgatatta atatttttca tgttattaaa taaagatata agagagtttt tctgatcttc     7380

tgtgagtgtc acgccaagtt cggaagcagc cgaatcaatc gttttctcca attcctcttt     7440

tgactcggga actccgtttt tcgagatttc ttccttgact ttggccatca gcgctgacgc     7500

gttttcactg ccgattttct cgccaagctc tgaagtggtg acaagctctt cattcgcgac     7560

ctttttcaca tcttcggaaa ttttttcgcc cgaagtcgtt tcatacgctt tcatcaatcc     7620

ggttaaagcg gctgtgcctg acacttcaaa cggagcggtg acatagactt tggcgtcttt     7680

tacaccggcc gtcatcagcg cgttcaaata catctcatct gtaattctgc tgatattgtg     7740

tgtctgaact tccaaaccgg tgcctttttt cgctacggta attgaagaag aagaaatcgc     7800

tcttgttccg atttgtgctt tcggtatata atcccctaaa tatttatgct cctcatcatt     7860

tgtcacctcg atgatggtcg cattttcagg cgcattcatt tcttttaata ctttttgtct     7920

gtcctggctt gacaagtctt tccccagcgt gacgatgaca tcacccactg cggcgtcagc     7980

gaagctgacc tgcgggaaaa tgagcagaca caatgctgta aagattccta gtatcgattt     8040

tttcaagctc aatgccctcc ttaaaaatgc aggcttcagg cagaattgct gtacttttaa     8100

agaagcctgc cggaacggaa ataatgcgtt ccgaaatata gacggatgaa agatgagtga     8160

ggtttcaaag aaaaaaagag agaattttct cttcaagtca aatgccctcc cggcatcgta     8220

tctcgccgct cttttatcat tcatgatttt cacaggcgat tcaacctttt tttaaaattt     8280

tttacaaaaa cgatacaaga gcggcgttta tttcggtcga ttggctctct gcttcttcaa     8340

tatgatataa tgacccttgt gaaatgaaag gagagaatca agatggctaa aaaaggatac     8400

atacaactga caaacggcaa aaaaatcgag tttgaactat atccggatgc ggcgccggga     8460

actgtcgcca actttgaaaa acttgcaaac gaagggttct atgacgggct gaagttccac     8520

cgcgtcatcc cgggcttcgt cagccaggga ggctgcccgc acggcaccgg aacaggcgga     8580

cctggatata cgattaaatg cgagacagaa gggaatccgc acaaacacga agccggttct     8640

ctctcaatgg ctcacgcagg aaaagatacc ggaggcagcc aattttttat cgtccatgag     8700

cctcagccgc acttgaacgg cgttcacacc gttttcggaa aggtcacatc aggccttgat     8760

gccgtcactt caatggagca gggacaaggc atggaaaaag tcgaagtatt tgatgcataa     8820

tcagagagcg caaaaaacag cccgcttagc cgggctgttt ttttgtctgt aacggtgttt     8880

attttccagg tgcaacagga cttgaggccg attcttcgtc cacatcctga taggaaataa     8940

cgatgctaat aaataaaata attgtgaaaa aatgaccctt tatgtaaaat atattcaagt     9000

gaagagctag atagagaacg caatctgtaa aaaaggaagg ggcgtaaggg gtgagcgtaa     9060

aaatcccatc gacggcagtc ggcgtaaaaa ttaatgactg gtataacgcg atacggttga     9120

acattcttag gctggtcgaa tcatttagac acgggcatcg tcctctcgaa aggtg          9175


<210>  22
<211>  84
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic

<400>  22

Phe Tyr Thr Phe Thr Tyr Phe Tyr Thr Phe Tyr Thr Phe Tyr Thr Phe 
1               5                   10                  15      


Thr Tyr Phe Tyr Thr Phe Tyr Thr Phe Tyr Thr Phe Thr Tyr Phe Tyr 
            20                  25                  30          


Thr Phe Tyr Thr Phe Tyr Thr Phe Thr Tyr Phe Tyr Thr Phe Tyr Thr 
        35                  40                  45              


Phe Tyr Thr Phe Thr Tyr Phe Tyr Thr Phe Tyr Thr Phe Tyr Thr Phe 
    50                  55                  60                  


Thr Tyr Phe Tyr Thr Phe Tyr Thr Phe Tyr Thr Phe Thr Tyr Phe Tyr 
65                  70                  75                  80  


Thr Phe Tyr Thr 
                


<210>  23
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette

<400>  23
aaaaaaaaaa aaattttttt ttttttgggg gggggggggc cccccccccc cc               52


<210>  24
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic promoter

<400>  24
gctgataaac agctgacatc aactaaaagt ttcattaaat actttgaaaa aagttgttga       60

cttaaaagaa gctaaatgtt atagtaattg t                                      91


<210>  25
<211>  1127
<212>  DNA
<213>  Bacillus licheniformis

<400>  25
catccgacca taatggaacg gattcaaaat attgaacagt cttcaactga atgaagaaaa       60

agctagtgct aaaaacacta gctttttcta tatgccattt gttagaatat gttattgagc      120

ggcagcttcg acattgatca gaccttttcc atagtagaag gagcttccca aataagtcgc      180

cgtactggag agacggttgc ggacttgtga agctgaaagg ttcggatgtt ttgacaagat      240

caaagctgct gctcccgcta catgaggaga agccattgac gttccgttca atgttgcata      300

agtgctggtt gggtaagtgc tgtatacgcc tgcgccagga gccatgactt caagctctgc      360

tccgacgctg gaaaatgaag ctctgttgct gttagagtct accgcaccaa cagcgatgac      420

agaatcgtat ttcgcaggat agccaattgt attcgtgttt cctgaagatc cgctgttccc      480

tgctgcagct acaacgacaa cccctcttgc atatgcattg tccctatttt tttggaggac      540

caatttaaaa ttttttggtt attaataact gttttagaaa aatgttcagt tcgaaagtat      600

gaatagaccg cttcagcctg gcagggaaag aggtcccgag ggccgcgaat gacaggagat      660

tgctccatct gattcatttt atgaaaatac tattcattta ttttatgaga tcattttttg      720

ccgaaggagg ttgcggcctt gtcgatcatc tattaaaaca tgcaatatat cgcatacaga      780

aacgggtgaa agatatgaaa tcagctcatg tgaaaggtgt attgatggtg cttgcagggg      840

cttcattgtg gggattatcg ggaagcgctg cacagtacat atttgaaagg ggagcggttg      900

aagccggctc gcttgtctct gtcaggcttc tggcatcggg agtcattctc ttgttatatg      960

tttcaatgaa aaacggtttt caacatgtgt gtaagatatg gaagaaaaaa acagatattt     1020

gttcgatttt agtattttcc atttttggaa tgcttgctgt tcaatatacg ttttttgctt     1080

caatcgaaaa gggtaatgcc gctgctgctg caattcttca atatttg                   1127


<210>  26
<211>  2472
<212>  DNA
<213>  Bacillus sp.

<400>  26
gcagctaaac ccgctgtaag caacgcttat ttagatgctt caaaccaggt gctggttaaa       60

cttagccagc cgttaactct tggggaaggc gcaagcggct ttacggttca tgacgacaca      120

gcaaataagg atattccagt gacatctgtg aaggatgcaa gtcttggtca agatgtaacc      180

gctgttttgg caggtacctt ccaacatatt tttggaggtt ccgattgggc acctgataat      240

cacagtactt tattaaaaaa ggtgactaac aatctctatc aattctcagg agatcttcct      300

gaaggaaact accaatataa agtggcttta aatgatagct ggaataatcc gagttaccca      360

tctgacaaca ttaatttaac agtccctgcc ggcggtgcac acgtcacttt ttcgtatatt      420

ccgtccactc atgcagtcta tgacacaatt aataatccta atgcggattt acaagtagaa      480

agcggggtta aaacggatct cgtgacggtt actctagggg aagatccaga tgtgagccat      540

actctgtcca ttcaaacaga tggctatcag gcaaagcagg tgatacctcg taacgtgctt      600

aattcatcac agtactacta ttcaggagat gatcttggga atacctatac acagaaagca      660

acaaccttta aagtctgggc accaacttct actcaagtaa atgttcttct ttatgacagt      720

gcaacgggtt ctgtaacaaa aatcgtacct atgacggcat cgggccatgg tgtgtgggaa      780

gcaacggtta atcaaaacct tgaaaattgg tattacatgt atgaggtaac aggccaaggc      840

tctacccgaa cggctgttga tccttatgca actgcgattg caccaaatgg aacgagaggc      900

atgattgtgg acctggctaa aacagatcct gctggctgga acagtgataa acatattacg      960

ccaaagaata tagaagatga ggtcatctat gaaatggatg tccgtgactt ttccattgac     1020

cctaattcgg gtatgaaaaa taaagggaag tatttggctc ttacagaaaa aggaacaaag     1080

ggccctgaca acgtaaagac ggggatagat tccttaaaac aacttgggat tactcatgtt     1140

cagcttatgc ctgttttcgc atctaacagt gtcgatgaaa ctgatccaac ccaagataat     1200

tggggttatg accctcgcaa ctatgatgtt cctgaagggc agtatgctac aaatgcgaat     1260

ggtaatgctc gtataaaaga gtttaaggaa atggttcttt cactccatcg tgaacacatt     1320

ggggttaaca tggatgttgt ctataatcat acctttgcca cgcaaatctc tgacttcgat     1380

aaaattgtac cagaatatta ttaccgtacg gatgatgcag gtaattatac caacggatca     1440

ggtactggaa atgaaattgc agccgaaagg ccaatggttc aaaaatttat tattgattcc     1500

cttaagtatt gggtcaatga gtatcatatt gacggcttcc gttttgactt aatggcgctg     1560

cttggaaaag acacgatgtc caaagctgcc tcggagcttc atgctattaa tccaggaatt     1620

gcactttacg gtgagccatg gacgggtgga acctctgcac tgccagatga tcagcttctg     1680

acaaaaggag ctcaaaaagg catgggagta gcggtgttta atgacaattt acgaaacgcg     1740

ttggacggca atgtctttga ttcttccgct caaggttttg cgacaggtgc aacaggctta     1800

actgatgcaa ttaagaatgg cgttgagggg agtattaatg actttacctc ttcaccaggt     1860

gagacaatta actatgtcac aagtcatgat aactacaccc tttgggacaa aatagcccta     1920

agcaatccta atgattccga agcggatcgg attaaaatgg atgaactcgc acaagcagtt     1980

gttatgacct cacaaggcgt tccattcatg caaggcgggg aagaaatgct tcgtacaaaa     2040

ggcggcaacg acaatagtta taatgcaggc gatgcggtca atgagtttga ttggagcagg     2100

aaagctcaat atccagatgt tttcaactat tatagcgggc taatccacct tcgtcttgat     2160

cacccagcct tccgcatgac gacagctaat gaaatcaata gccacctcca attcctaaat     2220

agtccagaga acacagtggc ctatgaatta actgatcatg ttaataaaga caaatgggga     2280

aatatcattg ttgtttataa cccaaataaa actgtagcaa ccatcaattt gccgagcggg     2340

aaatgggcaa tcaatgctac gagcggtaag gtaggagaat ccacccttgg tcaagcagag     2400

ggaagtgtcc aagtaccagg tatatctatg atgatccttc atcaagaggt aagcccagac     2460

cacggtaaaa ag                                                         2472


<210>  27
<211>  5913
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette

<400>  27
aaatataagg aggccaatta aatgatgaca gcaaaaaatt tgcaagatcg aatcaaattg       60

aataacggga cagctatgcc ttggtttgga cttggcgtat ttaaagtaga agaagggccc      120

gaactggtac aagccgtaaa aacggcgatt aaacacggct accgcagtat tgataccgca      180

gccatctacg gtaatgaaga gggggttggg caaggaatcc gcgaggggtt gaaagaagcc      240

ggcatttcaa gagaagacct gtttgttaca tcaaaggtct ggaatgacga tttaggctat      300

gacgaaacga ttgcagccta tgaggcgagt ctcgaaaagc tcggacttga ctaccttgat      360

ttatacctga tccactggcc tgttgaagga cgctacaaag cggcgtggaa agcgcttgaa      420

acactttatg aacaaggacg cgtaaaagca atcggagtga gcaattttca gattcaccat      480

ctggaagact tgctgaaaga tgccgccgtc aaaccggcga tcaaccaggt tgagtatcat      540

ccgcggctga cgcagaaaga gctgcaagcg ttttgccgtg cgcacggcat ccagctgcaa      600

gcatggtcgc cgctgatgca aggccaattg ctcagccatc cactgctgaa agatatcgcg      660

gacaagtacg gcaagacacc ggcccaagtc attttgcgct gggatttgca aaacggggtc      720

gttacgattc cgaagtcgac taaagcggag cggattgccc aaaacgcgga catatttgat      780

tttgaactga ccaccgagga aatgaagcaa attgacgcgc tgaatgaaaa cacccgtgtc      840

ggccctgatc ccgataactt tgacttttaa caaaacggcc ccgttcgaca ttcgaacggg      900

gctttaattg aattgtgcgg ttacaccgcc ggactccatc atcatcagtt cttttttcat      960

atccaatccg ccccggtatc ccgtgagctg cccgctttta ccgataaccc gatggcaagg     1020

caccaccatt aacagcggat ttgcgccgat cgccgcgcct actgcccgca cagcggcctg     1080

cttttcaata tgctcggcga tatcggaata ggagcaagtg ctgccgtaag ggatttcgga     1140

gagcgccttc cacactgcca gctgaaaagg cgtgccggca aggtcgacag gaaagctgaa     1200

atgagttcgc ttgccgttca aatacgcctg cagctgctcg gcgtattctg ccaatccttt     1260

gtcatcccga atgaaaactg gctgtgtaaa tcttttttca gcccaagcgg ccaaatcctc     1320

gaagccttga ttccatcccc ctgtaaaaca gagcccgcgg gcagtcgccc caatgtgaat     1380

ctgccaacct cggcaaataa gcgtacgcca gtatacgatt tgatcgtcca tatgtttacc     1440

tccgtttcat ttgccggtac gacgtcggcg attgcccagt cttcttttta aacaaagagg     1500

caaaatattc cgcattcgca atgcctacca ttgaagcgat ttctgcgatc gatcgttctg     1560

aatgagcaag caaatcgacc gctttctcaa tccttttctg caggatgtat tctgccggcg     1620

agacgccttt gattcgttta aatgtccgct gcaggtgaaa agggctgata tggcacctgt     1680

cagccaaagc ttgcagagac agcggatcgc gataagattc ctcgatgatt tccaccacac     1740

gctgtgccag ctcttcatcc ggcagcagcg ccccggccgg attgcagcgt ttgcaggggc     1800

ggtacccttc tgataaagca tcttttgcat tgaaaaagat ctgcacattg tcgatttgcg     1860

gaactctcga tttgcaggaa gggcggcaaa atatgccggt cgttttgacc gcgtaataaa     1920

aaactccgtc ataggcggaa tcgttttccg taatcgcccg ccacatttca ggcgtcaatc     1980

gtgatttgct gttcatatct tcaccccgat ctatgtcagt ataacctata tgacagccgg     2040

aggtggagag gcggagaacg gcacagcaag aagacaaaga agaagagaga ctgttgcctg     2100

gacctccgaa acgcgctaca attcatttac aacacaggat ggggtgagaa tattgccgga     2160

atcagtgaag caggcctcct aaaataaaaa tctatatttt aggaggtaaa acatgaattt     2220

tcaaacaatc gagcttgaca catggtatag aaaatcttat tttgaccatt acatgaagga     2280

agcgaaatgt tctttcagca tcacggcaaa cgtcaatgtg acaaatttgc tcgccgtgct     2340

caagaaaaag aagctcaagc tgtatccggc ttttatttat atcgtatcaa gggtcattca     2400

ttcgcgccct gagtttagaa caacgtttga tgacaaagga cagctgggtt attgggaaca     2460

aatgcatccg tgctatgcga tttttcatca ggacgaccaa acgttttccg ccctctggac     2520

ggaatactca gacgattttt cgcagtttta tcatcaatat cttctggacg ccgagcgctt     2580

tggagacaaa aggggccttt gggctaagcc ggacatcccg cccaatacgt tttcagtttc     2640

ttctattcca tgggtgcgct tttcaaactt caatttaaac cttgataaca gcgaacactt     2700

gctgccgatt attacaaacg ggaaatactt ttcagaaggc agggaaacat ttttgcccgt     2760

ttccttgcaa gttcaccatg cagtgtgtga cggctatcat gccggcgctt ttataaacga     2820

gttggaacgg cttgccgccg attgtgagga gtggcttgtg tgacagagga aaggccgata     2880

tgattcggcc ttttttatat gtacttctta gcgggtctct aaaataacca aaaagcaagg     2940

actgctgaaa gggctgacat aagccttttg ccggcggtcc ttttttaatt ctgatttttc     3000

aaacttagcc ccctcgagaa atgtgcgcgg tccccctgtt tcagggggtt ttttagtttg     3060

aaaaaaactt tcgctactaa aaaaatttcg gccccgtatt tttttaaaaa aacataattg     3120

aatataaata atacagttaa aactactaag aatctgtaaa tagaagtaag ctgggccaaa     3180

aaataagaaa aaaaaactat agttaaacat ttaacaaatg tctgataatt gtttataata     3240

taaatacaga atagtctttt aagtaagtct actctgaatt tttttaaaag gagagggtaa     3300

agattgaata aatcagtcat accttcttta attacggttg gaaactttgt ttcgggcatc     3360

tcttcaatac tgcttgcatt cagagggtat ttattcttag ccattatatt tgttctgatc     3420

ggcgctatct tggacagcct tgacggcatg gccgcgcgca gattaaatgc cgtcagcccg     3480

tttggaaaag aattagattc tttgtctgat attatcactt tcggagtcgc cccggccatc     3540

atcacctatt caatcgtttt ttatgatgct ccgatcttag gccttccttc gacattgtta     3600

ttccctgttt gcggagcgct tcggctggcg cgatttaata ttcaatcaga gaaccaggat     3660

tattttacag gccttccgat caccgcagcc ggaacgattc tggtatgctt aaaccttttc     3720

tcagaaatcc tgggcaaaaa gccgttcatt ttgatcatgc tgttcctgtc ctttctcatg     3780

gtcagcaaaa tcaaagtcat cagcctgaag gggaaaaaac tattgaagaa agtgagctag     3840

aagagcagag aggacggatt tcctgaagga aatccgtttt tttattttta acatctctca     3900

ctgctgtgtg attttactca cggcatttgg aacgccggct ctcaacaaac tttctgtagt     3960

gaaaatcatg aaccaaacgg atcgtcggcc tgattaacag ctgaaagctg ccgatcacaa     4020

acatccatag tcccgccggc ttcagttcct cggagaaaaa gcagaagctc ccgacaagga     4080

ataaaaggcc gatgagaaaa tcgtttaatg tatgtagaac tttgtatctt tttttgaaaa     4140

agagttcata tcgattgtta ttgttttgcg gcattgcttg atcactccaa tccttttatt     4200

taccctgccg gaagccggag tgaaacgccg gtatacatag gatttatgaa ttaggaaaac     4260

atatggggaa ataaaccatc caggagtgaa aaatatgcgg ttattcatat gtgcatcgtg     4320

cctgttcggc ttgattgttc cgtcatttga aacgaaagcg ctgacgtttg aagaattgcc     4380

ggttaaacaa gcttcaaaac aatgggaagt tcaaatcggt aaagccgaag ccggaaacgg     4440

aatggcgaaa ccggaaaaag gagcgtttca tacttatgct gtcgaaatca aaaacattgg     4500

acacgatgtg gcttcggcgg aaatttttgt ctatcggaac gagcctaatt cttcaacgaa     4560

attttcgctt tggaacattc ctcacgaaaa tccggtttct ttagccaaaa gcttaaatca     4620

cggaagctct gtcaagcacc gcaatctgct tatggcagag aatgcgaccg aattggaagt     4680

ggacatgatt tggacggaaa aaggaagcga aggcagactt ttaaaggaaa cgttcatttt     4740

caagggagat gaatcatgaa gaaaaaatgg ccgttcatcg tcaacggtct ttttttaatg     4800

acttaggcag ccgatcgttc ggccatacga tatcgaagcg acctcgaacc agcagagctc     4860

gtcacaaaac atttgcattt aaagaaaaat acaggatgtt ttcaccaata tttttctcaa     4920

tgatgataca ctattgacaa gctgctactt tgggagggtg tttccataga tgccgatgaa     4980

gcaaaaacac caaatgtgtc atgagagctc tctctaatcg atataaaagt agggtgaacc     5040

ggggttgtca atctgtaaaa gatctttttt tatcccgtga tacgcttttg gaattctgaa     5100

tcttcaagaa agtccccagc cttttgctga tcaatcgaga acaaaggatg atacatatga     5160

aaagaataga taaaatctac catcagctgc tggataattt tcgcgaaaag aatatcaatc     5220

agcttttaaa gatacaaggg aattcggcta aagaaatcgc cgggcagctg caaatggagc     5280

gttccaatgt cagctttgaa ttaaacaatc tcgttcgggc caaaaaggtg atcaagatta     5340

aaacgttccc cgtccgctac atcccggtgg aaattgttga aaacgtcttg aacatcaaat     5400

ggaattcaga gttgatggag gttgaagaac tgaggcggct ggctgacggc caaaaaaagc     5460

cggcgcgcaa tatatccgcc gatcccctcg agctcatgat cggggctaaa gggagcttga     5520

aaaaggcaat ttctcaggcg aaagcggcag tcttttatcc tccgcacggc ttgcatatgc     5580

tgctgctcgg gccgacgggt tcggggaaat cgctgtttgc gaatcggatc taccagttcg     5640

ccgtttattc tgacatattg aagcccgatt ccccgttcat cacattcaac tgtgcagatt     5700

actataacaa ccctcaatta ttgctctctc aattgttcgg acataaaaaa gggtctttta     5760

caggtgcggg tgaagacaaa gcaggattag tcgagcaggc ggacgggggc attctgttta     5820

tggatgaaat ccatcgcctc ccgccggagg ggcaggaaat gctgttttat ttcatagaca     5880

gcggcacata caacaggctt ggtgaaacag agc                                  5913


