                               SEQUENCE LISTING

<110> KIMBERLY-CLARK WORLDWIDE, INC.
 
<120> OPTIMIZATION OF A THERMOPHILIC PHB DEPOLYMERASE FOR INDUSTRIAL 
      APPLICATIONS

<130> KCX-2008-PCT

<140>
<141>

<160> 18    

<170> PatentIn version 3.5

<210> 1
<211> 322
<212> PRT
<213> Lihuaxuella thermophila

<400> 1
Met Phe Leu Leu Ala Leu Val Thr Ser Leu Ile Gly Thr Gly Met Phe 
1               5                   10                  15      


Thr Thr Pro Val His Ala Ala Gly Gln Phe Ile Arg Asp Thr Ala Pro 
            20                  25                  30          


Asp Gly Arg Val Tyr Lys Leu Tyr Ile Pro Ser Gly Tyr Asn Gly Ser 
        35                  40                  45              


Thr Pro Leu Pro Leu Val Val Met Leu His Gly Cys Thr Gln Asn Pro 
    50                  55                  60                  


Asp Asp Phe Ala Ala Gly Thr Glu Met Asn Val Tyr Ala Glu Gln Asn 
65                  70                  75                  80  


Asn Phe Leu Val Ala Tyr Pro Glu Gln Pro Ser Ser Ala Asn Leu Asn 
                85                  90                  95      


Lys Cys Trp Asn Trp Phe Asp Ser Asn His Gln Ser Arg Gly Arg Gly 
            100                 105                 110         


Glu Pro Ala Ser Ile Ala Gly Val Val Glu Asp Val Lys Arg Asn Tyr 
        115                 120                 125             


Ser Val Asp Ser Arg Arg Val Tyr Ala Ala Gly Leu Ser Ala Gly Gly 
    130                 135                 140                 


Ala Met Ser Val Ile Met Gly Ala Thr Tyr Pro Asp Val Phe Ala Ala 
145                 150                 155                 160 


Ile Gly Val Gly Ser Gly Leu Glu Tyr Lys Ala Ala Thr Ser Met Thr 
                165                 170                 175     


Ser Ala Tyr Met Ala Met Ile Asn Gly Gly Pro Asp Pro Val Gln Gln 
            180                 185                 190         


Gly Asn Leu Ala Tyr Gln Ala Met Gly Ser His Ala Arg Val Val Pro 
        195                 200                 205             


Val Ile Val Phe His Gly Thr Ser Asp Tyr Thr Val Tyr Pro Val Asn 
    210                 215                 220                 


Gly His Gln Val Ile Ser Gln Trp Ala Gln Thr Asn Asp Arg Ala Gly 
225                 230                 235                 240 


Asp Gly Val Asp Asn Asn His Ile Asp Asp Gln Ala Asp Val Thr Met 
                245                 250                 255     


Asn Gly Ser Val Pro Asn Gly Arg Thr Tyr Thr Arg Tyr Leu Tyr Lys 
            260                 265                 270         


Asp Gln Asn Gly Asn Val Val Met Glu Lys Ile Met Val Asn Gly Met 
        275                 280                 285             


Gly His Ala Trp Ser Gly Gly Ser Thr Ala Gly Thr Tyr Thr Asp Pro 
    290                 295                 300                 


Ala Gly Pro Glu Ala Ser Ser Met Met Trp Ser Phe Phe Val Asn His 
305                 310                 315                 320 


Pro Lys 
        


<210> 2
<211> 9
<212> PRT
<213> Lihuaxuella thermophila

<400> 2
Val Val Met Leu His Gly Cys Thr Gln 
1               5                   


<210> 3
<211> 7
<212> PRT
<213> Lihuaxuella thermophila

<400> 3
Thr Ser Ala Tyr Met Ala Met 
1               5           


<210> 4
<211> 6
<212> PRT
<213> Lihuaxuella thermophila

<400> 4
Gly Arg Thr Tyr Thr Arg 
1               5       


<210> 5
<211> 5
<212> PRT
<213> Lihuaxuella thermophila

<400> 5
Met Gly His Ala Trp 
1               5   


<210> 6
<211> 299
<212> PRT
<213> Lihuaxuella thermophila

<400> 6
Gly Gln Phe Ile Arg Asp Thr Ala Pro Asp Gly Arg Val Tyr Lys Leu 
1               5                   10                  15      


Tyr Ile Pro Ser Gly Tyr Asn Gly Ser Thr Pro Leu Pro Leu Val Val 
            20                  25                  30          


Met Leu His Gly Cys Thr Gln Asn Pro Asp Asp Phe Ala Ala Gly Thr 
        35                  40                  45              


Glu Met Asn Val Tyr Ala Glu Gln Asn Asn Phe Leu Val Ala Tyr Pro 
    50                  55                  60                  


Glu Gln Pro Ser Ser Ala Asn Leu Asn Lys Cys Trp Asn Trp Phe Asp 
65                  70                  75                  80  


Ser Asn His Gln Ser Arg Gly Arg Gly Glu Pro Ala Ser Ile Ala Gly 
                85                  90                  95      


Val Val Glu Asp Val Lys Arg Asn Tyr Ser Val Asp Ser Arg Arg Val 
            100                 105                 110         


Tyr Ala Ala Gly Leu Ser Ala Gly Gly Ala Met Ser Val Ile Met Gly 
        115                 120                 125             


Ala Thr Tyr Pro Asp Val Phe Ala Ala Ile Gly Val Gly Ser Gly Leu 
    130                 135                 140                 


Glu Tyr Lys Ala Ala Thr Ser Met Thr Ser Ala Tyr Met Ala Met Ile 
145                 150                 155                 160 


Asn Gly Gly Pro Asp Pro Val Gln Gln Gly Asn Leu Ala Tyr Gln Ala 
                165                 170                 175     


Met Gly Ser His Ala Arg Val Val Pro Val Ile Val Phe His Gly Thr 
            180                 185                 190         


Ser Asp Tyr Thr Val Tyr Pro Val Asn Gly His Gln Val Ile Ser Gln 
        195                 200                 205             


Trp Ala Gln Thr Asn Asp Arg Ala Gly Asp Gly Val Asp Asn Asn His 
    210                 215                 220                 


Ile Asp Asp Gln Ala Asp Val Thr Met Asn Gly Ser Val Pro Asn Gly 
225                 230                 235                 240 


Arg Thr Tyr Thr Arg Tyr Leu Tyr Lys Asp Gln Asn Gly Asn Val Val 
                245                 250                 255     


Met Glu Lys Ile Met Val Asn Gly Met Gly His Ala Trp Ser Gly Gly 
            260                 265                 270         


Ser Thr Ala Gly Thr Tyr Thr Asp Pro Ala Gly Pro Glu Ala Ser Ser 
        275                 280                 285             


Met Met Trp Ser Phe Phe Val Asn His Pro Lys 
    290                 295                 


<210> 7
<211> 299
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 7
Gly Gln Phe Ile Arg Asp Thr Ala Pro Leu Gly Arg Val Tyr Phe Leu 
1               5                   10                  15      


Tyr Ile Pro Ser Gly Tyr Asn Gly Ser Thr Pro Leu Pro Leu Val Val 
            20                  25                  30          


Met Leu His Gly Cys Thr Gln Asn Pro Asp Asp Phe Ala Ala Gly Thr 
        35                  40                  45              


Leu Met Asn Val Tyr Ala Glu Gln Asn Asn Phe Leu Val Ala Tyr Pro 
    50                  55                  60                  


Glu Gln Leu Ser Ser Ala Asn Leu Asn Lys Cys Trp Asn Trp Phe Asp 
65                  70                  75                  80  


Ser Asn His Gln Ser Arg Gly Arg Gly Glu Pro Ala Ser Ile Ala Gly 
                85                  90                  95      


Met Val Glu Leu Val Phe Arg Asn Tyr Ser Val Asp Ser Arg Arg Val 
            100                 105                 110         


Tyr Leu Ala Gly Leu Ser Ala Gly Gly Ala Met Ile Leu Ile Met Leu 
        115                 120                 125             


Ala Thr Tyr Pro Asp Val Phe Ala Ala Ile Phe Val Gly Ser Gly Leu 
    130                 135                 140                 


Pro Tyr Lys Leu Ala Thr Ser Met Thr Ser Ala Tyr Met Ala Met Ile 
145                 150                 155                 160 


Asn Gly Gly Pro Asp Pro Val Gln Gln Gly Asn Leu Ala Tyr Gln Ala 
                165                 170                 175     


Met Gly Ser His Ala Arg Val Val Pro Val Ile Val Phe His Gly Thr 
            180                 185                 190         


Ser Asp Tyr Thr Val Tyr Glu Val Asn Gly His Gln Val Ile Ser Gln 
        195                 200                 205             


Trp Ala Gln Thr Asn Asp Arg Ala Gly Asp Gly Val Asp Asn Asn His 
    210                 215                 220                 


Ile Asp Asp Gln Ala Asp Val Thr Met Asn Gly Ser Val Glu Asn Gly 
225                 230                 235                 240 


Arg Thr Tyr Thr Arg Tyr Leu Tyr Lys Asp Gln Asn Gly Asn Val Val 
                245                 250                 255     


Met Glu Lys Ile Met Val Asn Gly Met Gly His Ala Trp Ser Gly Gly 
            260                 265                 270         


Ser Thr Ala Gly Thr Tyr Thr Asp Pro Ala Gly Glu Glu Ala Ser Ser 
        275                 280                 285             


Met Met Trp Ser Phe Phe Val Asn His Glu Lys 
    290                 295                 


<210> 8
<211> 299
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 8
Gly Gln Val Ile Arg Asp Thr Ala Pro Asp Gly Arg Val Tyr Lys Leu 
1               5                   10                  15      


Tyr Ile Pro Ser Gly Tyr Asn Gly Ser Thr Pro Leu Pro Leu Val Val 
            20                  25                  30          


Val Leu His Gly Cys Thr Gln Asn Trp Asp Asp Ile Ala Ala Gly Thr 
        35                  40                  45              


Glu Leu Asn Val Tyr Ala Glu Gln Asn Asn Phe Leu Val Ala Tyr Pro 
    50                  55                  60                  


Glu Gln Pro Ser Ser Ala Asn Leu Asn Lys Cys Trp Asn Leu Phe Asp 
65                  70                  75                  80  


Ser Asn His Gln Ser Arg Gly Arg Gly Glu Leu Pro Ser Ile Ala Gly 
                85                  90                  95      


Val Val Glu Asp Val Lys Arg Asn Tyr Ser Val Asp Ser Arg Arg Val 
            100                 105                 110         


Tyr Val Ala Gly Leu Ser Ala Gly Gly Ile Leu Ser Val Ile Leu Gly 
        115                 120                 125             


Leu Thr Tyr Pro Asp Val Leu Ala Ala Ile Gly Val Gly Ser Gly Trp 
    130                 135                 140                 


Glu Tyr Lys Ala Ala Thr Ser Trp Thr Ser Ala Tyr Leu Ala Leu Gly 
145                 150                 155                 160 


Asn Gly Gly Pro Asp Pro Pro Gln Gln Gly Asn Leu Ala Tyr Gln Ala 
                165                 170                 175     


Met Gly Ser His Ala Arg Val Pro Pro Val Ile Val Trp His Gly Thr 
            180                 185                 190         


Ser Asp Tyr Thr Val Tyr Pro Pro Asn Gly His Gln Val Leu Ser Gln 
        195                 200                 205             


Ile Ala Gln Thr Asn Asp Arg Ala Gly Asp Gly Val Asp Asn Asn His 
    210                 215                 220                 


Ile Asp Asp Gln Ala Asp Val Thr Ile Asn Gly Ser Val Pro Asn Gly 
225                 230                 235                 240 


Arg Thr Tyr Thr Arg Tyr Leu Tyr Lys Asp Gln Asn Gly Asn Val Val 
                245                 250                 255     


Met Glu Lys Ile Leu Val Asn Gly Met Gly His Ala Val Ser Gly Gly 
            260                 265                 270         


Ser Thr Gly Gly Thr Tyr Thr Asp Pro Ala Gly Pro Glu Ala Ser Ser 
        275                 280                 285             


Met Met Trp Ser Phe Phe Val Asn His Pro Lys 
    290                 295                 


<210> 9
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 9
Met His His His His His His Gly Ser Glu Asn Leu Tyr Phe Gln Ser 
1               5                   10                  15      


<210> 10
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 10
Ser Ala Gly Gln Phe 
1               5   


<210> 11
<211> 299
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (10)..(10)
<223> L or F

<220>
<221> MOD_RES
<222> (15)..(15)
<223> F, L or W

<220>
<221> MOD_RES
<222> (49)..(49)
<223> L, I, V or F

<220>
<221> MOD_RES
<222> (62)..(62)
<223> L, W, Y, I, F, M, V or C

<220>
<221> MOD_RES
<222> (93)..(93)
<223> I or L

<220>
<221> MOD_RES
<222> (100)..(100)
<223> L or F

<220>
<221> MOD_RES
<222> (102)..(102)
<223> M, F or L

<220>
<221> MOD_RES
<222> (114)..(114)
<223> L, I, F, Y, V or M

<220>
<221> MOD_RES
<222> (124)..(124)
<223> I, L, T, A, F, M or V

<220>
<221> MOD_RES
<222> (128)..(128)
<223> L, F, I, W, A, M, V, E, T, C, Y or N

<220>
<221> MOD_RES
<222> (139)..(139)
<223> W, F, Y, L, I or P

<220>
<221> MOD_RES
<222> (148)..(148)
<223> L, C or F

<220>
<221> MOD_RES
<222> (169)..(169)
<223> L, W or I

<220>
<221> MOD_RES
<222> (202)..(202)
<223> L, F, I, V, M or W

<400> 11
Gly Gln Phe Ile Arg Asp Thr Ala Pro Xaa Gly Arg Val Tyr Xaa Leu 
1               5                   10                  15      


Tyr Ile Pro Ser Gly Tyr Asn Gly Ser Thr Pro Leu Pro Leu Val Val 
            20                  25                  30          


Met Leu His Gly Cys Thr Gln Asn Pro Asp Asp Phe Ala Ala Gly Thr 
        35                  40                  45              


Xaa Met Asn Val Tyr Ala Glu Gln Asn Asn Phe Leu Val Xaa Tyr Pro 
    50                  55                  60                  


Glu Gln Pro Ser Ser Ala Asn Leu Asn Lys Cys Trp Asn Trp Phe Asp 
65                  70                  75                  80  


Ser Asn His Gln Ser Arg Gly Arg Gly Glu Pro Ala Xaa Ile Ala Gly 
                85                  90                  95      


Met Val Glu Xaa Val Xaa Arg Asn Tyr Ser Val Asp Ser Arg Arg Val 
            100                 105                 110         


Tyr Xaa Ala Gly Leu Ser Ala Gly Gly Ala Met Xaa Val Ile Met Xaa 
        115                 120                 125             


Ala Thr Tyr Pro Asp Val Phe Ala Ala Ile Xaa Val Gly Ser Gly Leu 
    130                 135                 140                 


Pro Tyr Lys Xaa Ala Thr Ser Met Thr Ser Ala Tyr Met Ala Met Ile 
145                 150                 155                 160 


Asn Gly Gly Pro Asp Pro Val Gln Xaa Gly Asn Leu Ala Tyr Gln Ala 
                165                 170                 175     


Met Gly Ser His Ala Arg Val Val Pro Val Ile Val Phe His Gly Thr 
            180                 185                 190         


Ser Asp Tyr Thr Val Tyr Pro Val Asn Xaa His Gln Val Ile Ser Gln 
        195                 200                 205             


Trp Ala Gln Thr Asn Asp Arg Ala Gly Asp Gly Val Asp Asn Asn His 
    210                 215                 220                 


Ile Asp Asp Gln Ala Asp Val Thr Met Asn Gly Ser Val Pro Asn Gly 
225                 230                 235                 240 


Arg Thr Tyr Thr Arg Tyr Leu Tyr Lys Asp Gln Asn Gly Asn Val Val 
                245                 250                 255     


Met Glu Leu Trp Met Val Asn Gly Met Gly His Ala Trp Ser Gly Gly 
            260                 265                 270         


Ser Thr Ala Gly Thr Tyr Thr Asp Pro Ala Gly Pro Glu Ala Ser Ser 
        275                 280                 285             


Met Met Trp Ser Phe Phe Val Asn His Pro Lys 
    290                 295                 


<210> 12
<211> 299
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 12
Gly Gln Phe Ile Arg Asp Thr Ala Pro Leu Gly Arg Val Tyr Phe Leu 
1               5                   10                  15      


Tyr Ile Pro Ser Gly Tyr Asn Gly Ser Thr Pro Leu Pro Leu Val Val 
            20                  25                  30          


Met Leu His Gly Cys Thr Gln Asn Pro Asp Asp Phe Ala Ala Gly Thr 
        35                  40                  45              


Leu Met Asn Val Tyr Ala Glu Gln Asn Asn Phe Leu Val Leu Tyr Pro 
    50                  55                  60                  


Glu Gln Pro Ser Ser Ala Asn Leu Asn Lys Cys Trp Asn Trp Phe Asp 
65                  70                  75                  80  


Ser Asn His Gln Ser Arg Gly Arg Gly Glu Pro Ala Ile Ile Ala Gly 
                85                  90                  95      


Met Val Glu Leu Val Met Arg Asn Tyr Ser Val Asp Ser Arg Arg Val 
            100                 105                 110         


Tyr Leu Ala Gly Leu Ser Ala Gly Gly Ala Met Ile Val Ile Met Leu 
        115                 120                 125             


Ala Thr Tyr Pro Asp Val Phe Ala Ala Ile Trp Val Gly Ser Gly Leu 
    130                 135                 140                 


Pro Tyr Lys Leu Ala Thr Ser Met Thr Ser Ala Tyr Met Ala Met Ile 
145                 150                 155                 160 


Asn Gly Gly Pro Asp Pro Val Gln Leu Gly Asn Leu Ala Tyr Gln Ala 
                165                 170                 175     


Met Gly Ser His Ala Arg Val Val Pro Val Ile Val Phe His Gly Thr 
            180                 185                 190         


Ser Asp Tyr Thr Val Tyr Pro Val Asn Leu His Gln Val Ile Ser Gln 
        195                 200                 205             


Trp Ala Gln Thr Asn Asp Arg Ala Gly Asp Gly Val Asp Asn Asn His 
    210                 215                 220                 


Ile Asp Asp Gln Ala Asp Val Thr Met Asn Gly Ser Val Pro Asn Gly 
225                 230                 235                 240 


Arg Thr Tyr Thr Arg Tyr Leu Tyr Lys Asp Gln Asn Gly Asn Val Val 
                245                 250                 255     


Met Glu Leu Trp Met Val Asn Gly Met Gly His Ala Trp Ser Gly Gly 
            260                 265                 270         


Ser Thr Ala Gly Thr Tyr Thr Asp Pro Ala Gly Pro Glu Ala Ser Ser 
        275                 280                 285             


Met Met Trp Ser Phe Phe Val Asn His Pro Lys 
    290                 295                 


<210> 13
<211> 299
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (49)..(49)
<223> L or I

<220>
<221> MOD_RES
<222> (62)..(62)
<223> L, W, Y, I or F

<220>
<221> MOD_RES
<222> (100)..(100)
<223> L or F

<220>
<221> MOD_RES
<222> (114)..(114)
<223> L, I, F, Y or V

<220>
<221> MOD_RES
<222> (124)..(124)
<223> I, L, T or A

<220>
<221> MOD_RES
<222> (128)..(128)
<223> L, F, I, W, A, M, V or E

<220>
<221> MOD_RES
<222> (202)..(202)
<223> L, F or I

<400> 13
Gly Gln Phe Ile Arg Asp Thr Ala Pro Asp Gly Arg Val Tyr Phe Leu 
1               5                   10                  15      


Tyr Ile Pro Ser Gly Tyr Asn Gly Ser Thr Pro Leu Pro Leu Val Val 
            20                  25                  30          


Met Leu His Gly Cys Thr Gln Asn Pro Asp Asp Phe Ala Ala Gly Thr 
        35                  40                  45              


Xaa Met Asn Val Tyr Ala Glu Gln Asn Asn Phe Leu Val Xaa Tyr Pro 
    50                  55                  60                  


Glu Gln Pro Ser Ser Ala Asn Leu Asn Lys Cys Trp Asn Trp Phe Asp 
65                  70                  75                  80  


Ser Asn His Gln Ser Arg Gly Arg Gly Glu Pro Ala Ile Ile Ala Gly 
                85                  90                  95      


Met Val Glu Xaa Val Met Arg Asn Tyr Ser Val Asp Ser Arg Arg Val 
            100                 105                 110         


Tyr Xaa Ala Gly Leu Ser Ala Gly Gly Ala Met Xaa Val Ile Met Xaa 
        115                 120                 125             


Ala Thr Tyr Pro Asp Val Phe Ala Ala Ile Trp Val Gly Ser Gly Leu 
    130                 135                 140                 


Pro Tyr Lys Ala Ala Thr Ser Met Thr Ser Ala Tyr Met Ala Met Ile 
145                 150                 155                 160 


Asn Gly Gly Pro Asp Pro Val Gln Leu Gly Asn Leu Ala Tyr Gln Ala 
                165                 170                 175     


Met Gly Ser His Ala Arg Val Val Pro Val Ile Val Phe His Gly Thr 
            180                 185                 190         


Ser Asp Tyr Thr Val Tyr Pro Val Asn Xaa His Gln Val Ile Ser Gln 
        195                 200                 205             


Trp Ala Gln Thr Asn Asp Arg Ala Gly Asp Gly Val Asp Asn Asn His 
    210                 215                 220                 


Ile Asp Asp Gln Ala Asp Val Thr Met Asn Gly Ser Val Pro Asn Gly 
225                 230                 235                 240 


Arg Thr Tyr Thr Arg Tyr Leu Tyr Lys Asp Gln Asn Gly Asn Val Val 
                245                 250                 255     


Met Glu Lys Ile Met Val Asn Gly Met Gly His Ala Trp Ser Gly Gly 
            260                 265                 270         


Ser Thr Ala Gly Thr Tyr Thr Asp Pro Ala Gly Pro Glu Ala Ser Ser 
        275                 280                 285             


Met Met Trp Ser Phe Phe Val Asn His Pro Lys 
    290                 295                 


<210> 14
<211> 299
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (114)..(114)
<223> L or V

<400> 14
Gly Gln Phe Ile Arg Asp Thr Ala Pro Leu Gly Arg Val Tyr Phe Leu 
1               5                   10                  15      


Tyr Ile Pro Ser Gly Tyr Asn Gly Ser Thr Pro Leu Pro Leu Val Val 
            20                  25                  30          


Met Leu His Gly Cys Thr Gln Asn Pro Asp Asp Phe Ala Ala Gly Thr 
        35                  40                  45              


Leu Met Asn Val Tyr Ala Glu Gln Asn Asn Phe Leu Val Ala Tyr Pro 
    50                  55                  60                  


Glu Gln Pro Ser Ser Ala Asn Leu Asn Lys Cys Trp Asn Trp Phe Asp 
65                  70                  75                  80  


Ser Asn His Gln Ser Arg Gly Arg Gly Glu Pro Ala Ile Ile Ala Gly 
                85                  90                  95      


Met Val Glu Leu Val Phe Arg Asn Tyr Ser Val Asp Ser Arg Arg Val 
            100                 105                 110         


Tyr Xaa Ala Gly Leu Ser Ala Gly Gly Ala Met Ile Val Ile Met Leu 
        115                 120                 125             


Ala Thr Tyr Pro Asp Val Phe Ala Ala Ile Phe Val Gly Ser Gly Leu 
    130                 135                 140                 


Pro Tyr Lys Ala Ala Thr Ser Met Thr Ser Ala Tyr Met Ala Met Ile 
145                 150                 155                 160 


Asn Gly Gly Pro Asp Pro Val Gln Gln Gly Asn Leu Ala Tyr Gln Ala 
                165                 170                 175     


Met Gly Ser His Ala Arg Val Val Pro Val Ile Val Phe His Gly Thr 
            180                 185                 190         


Ser Asp Tyr Thr Val Tyr Pro Val Asn Gly His Gln Val Ile Ser Gln 
        195                 200                 205             


Trp Ala Gln Thr Asn Asp Arg Ala Gly Asp Gly Val Asp Asn Asn His 
    210                 215                 220                 


Ile Asp Asp Gln Ala Asp Val Thr Met Asn Gly Ser Val Pro Asn Gly 
225                 230                 235                 240 


Arg Thr Tyr Thr Arg Tyr Leu Tyr Lys Asp Gln Asn Gly Asn Val Val 
                245                 250                 255     


Met Glu Lys Ile Met Val Asn Gly Met Gly His Ala Trp Ser Gly Gly 
            260                 265                 270         


Ser Thr Ala Gly Thr Tyr Thr Asp Pro Ala Gly Pro Glu Ala Ser Ser 
        275                 280                 285             


Met Met Trp Ser Phe Phe Val Asn His Pro Lys 
    290                 295                 


<210> 15
<211> 299
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (124)..(124)
<223> I, L, T, A, F, M or V

<220>
<221> MOD_RES
<222> (128)..(128)
<223> L, V, I, W, A, M, V, E, T, C, Y or N

<400> 15
Gly Gln Phe Ile Arg Asp Thr Ala Pro Asp Gly Arg Val Tyr Lys Leu 
1               5                   10                  15      


Tyr Ile Pro Ser Gly Tyr Asn Gly Ser Thr Pro Leu Pro Leu Val Val 
            20                  25                  30          


Met Leu His Gly Cys Thr Gln Asn Pro Asp Asp Phe Ala Ala Gly Thr 
        35                  40                  45              


Glu Met Asn Val Tyr Ala Glu Gln Asn Asn Phe Leu Val Ala Tyr Pro 
    50                  55                  60                  


Glu Gln Pro Ser Ser Ala Asn Leu Asn Lys Cys Trp Asn Trp Phe Asp 
65                  70                  75                  80  


Ser Asn His Gln Ser Arg Gly Arg Gly Glu Pro Ala Ser Ile Ala Gly 
                85                  90                  95      


Val Val Glu Asp Val Lys Arg Asn Tyr Ser Val Asp Ser Arg Arg Val 
            100                 105                 110         


Tyr Ala Ala Gly Leu Ser Ala Gly Gly Ile Leu Xaa Leu Ile Leu Xaa 
        115                 120                 125             


Leu Thr Tyr Pro Asp Val Phe Ala Ala Ile Gly Val Gly Ser Gly Leu 
    130                 135                 140                 


Glu Tyr Lys Ala Ala Thr Ser Met Thr Ser Ala Tyr Met Ala Met Ile 
145                 150                 155                 160 


Asn Gly Gly Pro Asp Pro Val Gln Gln Gly Asn Leu Ala Tyr Gln Ala 
                165                 170                 175     


Met Gly Ser His Ala Arg Val Val Pro Val Ile Val Phe His Gly Thr 
            180                 185                 190         


Ser Asp Tyr Thr Val Tyr Pro Val Asn Gly His Gln Val Ile Ser Gln 
        195                 200                 205             


Trp Ala Gln Thr Asn Asp Arg Ala Gly Asp Gly Val Asp Asn Asn His 
    210                 215                 220                 


Ile Asp Asp Gln Ala Asp Val Thr Met Asn Gly Ser Val Pro Asn Gly 
225                 230                 235                 240 


Arg Thr Tyr Thr Arg Tyr Leu Tyr Lys Asp Gln Asn Gly Asn Val Val 
                245                 250                 255     


Met Glu Lys Ile Met Val Asn Gly Met Gly His Ala Trp Ser Gly Gly 
            260                 265                 270         


Ser Thr Ala Gly Thr Tyr Thr Asp Pro Ala Gly Pro Glu Ala Ser Ser 
        275                 280                 285             


Met Met Trp Ser Phe Phe Val Asn His Pro Lys 
    290                 295                 


<210> 16
<211> 108
<212> PRT
<213> Escherichia coli

<400> 16
Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp Val 
1               5                   10                  15      


Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp Cys 
            20                  25                  30          


Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp Glu 
        35                  40                  45              


Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn Pro 
    50                  55                  60                  


Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu 
65                  70                  75                  80  


Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser Lys 
                85                  90                  95      


Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala 
            100                 105             


<210> 17
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 17
His His His His His His 
1               5       


<210> 18
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 18
Gly Ser Gly Ser Gly Ser 
1               5       


