                               SEQUENCE LISTING

<110> KIMBERLY-CLARK WORLDWIDE, INC.
 
<120> OPTIMIZATION OF A HALOPHILIC PHB DEPOLYMERASE FOR INDUSTRIAL 
      APPLICATIONS

<130> KCX-2016-PCT

<140>
<141>

<160> 19    

<170> PatentIn version 3.5

<210> 1
<211> 355
<212> PRT
<213> Halomonas aquamarina

<400> 1
Met Gly Ile Ala Val His Gln Arg Arg Leu Met Ala Ala Leu Ile Leu 
1               5                   10                  15      


Leu Gly Ser Ala Val Ala His Ala Glu Glu Glu Ala Pro Gly Leu Pro 
            20                  25                  30          


Ala Leu Gly Ala Ala Asn Asp Gln Ala Ser Val Val Gly Val Ser Ser 
        35                  40                  45              


Gly Gly Tyr Met Ala Ser Gln Leu Ala Val Ala Trp Pro Glu Arg Phe 
    50                  55                  60                  


Ser Gly Val Gly Met Leu Ala Ala Gly Pro Trp Gly Cys Ala Gln Gly 
65                  70                  75                  80  


Ala Leu Ser Leu Ala Leu Asn Gln Cys Met Met Thr Arg Arg Gly Leu 
                85                  90                  95      


Pro Ser Leu Asp Glu Leu Glu Gln Arg Arg Glu Arg Tyr Leu Ser Leu 
            100                 105                 110         


Asp Gln Val Gly Ser Gln Asp Ala Leu Ser Gln Leu Arg Ala Phe Val 
        115                 120                 125             


Trp His Gly Asp Ala Asp Glu Thr Val Ser Pro Ala Leu Gly Asp Leu 
    130                 135                 140                 


Leu Ala Gln Gln Trp Gln Gly Trp Leu Glu Ser Pro Glu Gln Gln Leu 
145                 150                 155                 160 


Arg Tyr Val Gln Arg Ala Asn Thr Gly His Gly Trp Pro Val Ala Met 
                165                 170                 175     


Pro Lys Asp Ala Pro Ile Asp Pro Gln Ser Leu Gly Asp Cys Arg Asn 
            180                 185                 190         


Gly Gly Gly Ser His Val Leu Ala Cys Gly Glu Asp Val Ala Gly Glu 
        195                 200                 205             


Met Met Ala Trp Leu Tyr Pro Glu Arg Glu Thr Asn Ala Ser Glu Gly 
    210                 215                 220                 


Glu Leu Leu Ala Phe Asp Gln Ser Asp Phe Ala Ala Lys Gly Phe Ala 
225                 230                 235                 240 


Asp Thr Gly Tyr Val Phe Val Pro Glu Ala Cys Glu Ala Gly Gly Cys 
                245                 250                 255     


Pro Val Thr Val Ala Leu His Gly Cys Gln Met Asn Ala Glu Ala Ile 
            260                 265                 270         


Asp Asp Thr Phe Val Arg Tyr Ser Gly Leu Asn Arg Trp Ala Ala Glu 
        275                 280                 285             


His Gly Gln Val Val Leu Tyr Pro Gln Ala Glu Ser Ser Met Ala Asn 
    290                 295                 300                 


Pro Gln Ala Cys Trp Asp Trp Trp Gly Phe Ala Glu Ser Thr Trp Gln 
305                 310                 315                 320 


Ile Asn Pro Leu His Asp Thr Arg Asp Gly Thr Gln Thr Gln Ala Leu 
                325                 330                 335     


Met Ala Met Leu Asp His Leu Gln Ser Ala Thr Ala Asn Lys Ala Ala 
            340                 345                 350         


Thr Ala Glu 
        355 


<210> 2
<211> 9
<212> PRT
<213> Halomonas aquamarina

<400> 2
Ala Ala Gly Pro Trp Gly Cys Ala Gln 
1               5                   


<210> 3
<211> 10
<212> PRT
<213> Halomonas aquamarina

<400> 3
Arg Ala Phe Val Trp His Gly Asp Ala Asp 
1               5                   10  


<210> 4
<211> 7
<212> PRT
<213> Halomonas aquamarina

<400> 4
Gly Gly Cys Pro Val Thr Val 
1               5           


<210> 5
<211> 332
<212> PRT
<213> Halomonas aquamarina

<400> 5
Met Glu Glu Glu Ala Pro Gly Leu Pro Ala Leu Gly Ala Ala Asn Asp 
1               5                   10                  15      


Gln Ala Ser Val Val Gly Val Ser Ser Gly Gly Tyr Met Ala Ser Gln 
            20                  25                  30          


Leu Ala Val Ala Trp Pro Glu Arg Phe Ser Gly Val Gly Met Leu Ala 
        35                  40                  45              


Ala Gly Pro Trp Gly Cys Ala Gln Gly Ala Leu Ser Leu Ala Leu Asn 
    50                  55                  60                  


Gln Cys Met Met Thr Arg Arg Gly Leu Pro Ser Leu Asp Glu Leu Glu 
65                  70                  75                  80  


Gln Arg Arg Glu Arg Tyr Leu Ser Leu Asp Gln Val Gly Ser Gln Asp 
                85                  90                  95      


Ala Leu Ser Gln Leu Arg Ala Phe Val Trp His Gly Asp Ala Asp Glu 
            100                 105                 110         


Thr Val Ser Pro Ala Leu Gly Asp Leu Leu Ala Gln Gln Trp Gln Gly 
        115                 120                 125             


Trp Leu Glu Ser Pro Glu Gln Gln Leu Arg Tyr Val Gln Arg Ala Asn 
    130                 135                 140                 


Thr Gly His Gly Trp Pro Val Ala Met Pro Lys Asp Ala Pro Ile Asp 
145                 150                 155                 160 


Pro Gln Ser Leu Gly Asp Cys Arg Asn Gly Gly Gly Ser His Val Leu 
                165                 170                 175     


Ala Cys Gly Glu Asp Val Ala Gly Glu Met Met Ala Trp Leu Tyr Pro 
            180                 185                 190         


Glu Arg Glu Thr Asn Ala Ser Glu Gly Glu Leu Leu Ala Phe Asp Gln 
        195                 200                 205             


Ser Asp Phe Ala Ala Lys Gly Phe Ala Asp Thr Gly Tyr Val Phe Val 
    210                 215                 220                 


Pro Glu Ala Cys Glu Ala Gly Gly Cys Pro Val Thr Val Ala Leu His 
225                 230                 235                 240 


Gly Cys Gln Met Asn Ala Glu Ala Ile Asp Asp Thr Phe Val Arg Tyr 
                245                 250                 255     


Ser Gly Leu Asn Arg Trp Ala Ala Glu His Gly Gln Val Val Leu Tyr 
            260                 265                 270         


Pro Gln Ala Glu Ser Ser Met Ala Asn Pro Gln Ala Cys Trp Asp Trp 
        275                 280                 285             


Trp Gly Phe Ala Glu Ser Thr Trp Gln Ile Asn Pro Leu His Asp Thr 
    290                 295                 300                 


Arg Asp Gly Thr Gln Thr Gln Ala Leu Met Ala Met Leu Asp His Leu 
305                 310                 315                 320 


Gln Ser Ala Thr Ala Asn Lys Ala Ala Thr Ala Glu 
                325                 330         


<210> 6
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 6
Met His His His His His His Gly Ser Glu Asn Leu Tyr Phe Gln Ser 
1               5                   10                  15      


<210> 7
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 7
Ser Met Glu Glu Glu 
1               5   


<210> 8
<211> 332
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 8
Met Glu Glu Glu Ala Pro Gly Leu Pro Ala Leu Gly Ala Ala Asn Asp 
1               5                   10                  15      


Gln Ala Ser Val Val Gly Val Ser Ser Gly Gly Tyr Met Ala Ser Gln 
            20                  25                  30          


Leu Ala Val Ala Trp Pro Glu Arg Phe Ser Gly Val Gly Met Leu Ala 
        35                  40                  45              


Ala Gly Pro Trp Gly Cys Ala Gln Gly Ser Leu Ser Leu Ala Leu Asn 
    50                  55                  60                  


Gln Cys Met Met Thr Arg Arg Gly Leu Pro Ser Leu Asp Glu Leu Glu 
65                  70                  75                  80  


Gln Arg Arg Glu Arg Tyr Leu Ser Ser Asp Gln Val Gly Ser Gln Asp 
                85                  90                  95      


Ala Leu Ser Gln Leu Arg Ala Phe Val Trp His Gly Asp Ala Asp Glu 
            100                 105                 110         


Thr Val Ser Pro Ala Leu Gly Asp Leu Leu Ala Gln Gln Trp Gln Gly 
        115                 120                 125             


Trp Leu Glu Ser Pro Glu Gln Gln Leu Arg Tyr Val Gln Ser Ala Asn 
    130                 135                 140                 


Thr Gly His Gly Trp Pro Val Ala Met Pro Lys Asp Ala Pro Ser Asp 
145                 150                 155                 160 


Pro Gln Ser Leu Gly Asp Cys Arg Asn Gly Gly Gly Ser His Val Leu 
                165                 170                 175     


Ala Cys Gly Glu Asp Val Ala Gly Glu Met Met Ala Trp Leu Tyr Pro 
            180                 185                 190         


Glu Arg Glu Thr Asn Ala Ser Glu Gly Glu Leu Leu Ala Phe Asp Gln 
        195                 200                 205             


Ser Asp Phe Ala Ala Lys Gly Phe Ala Asp Thr Gly Tyr Val Phe Val 
    210                 215                 220                 


Pro Glu Ala Cys Glu Ala Gly Gly Cys Pro Val Thr Val Ala Leu His 
225                 230                 235                 240 


Gly Cys Gln Met Asn Ala Glu Ala Ile Asp Asp Thr Phe Val Arg Tyr 
                245                 250                 255     


Ser Gly Leu Asn Arg Trp Ala Ala Glu His Gly Gln Val Val Leu Tyr 
            260                 265                 270         


Pro Gln Ala Glu Ser Ser Met Ala Asn Pro Gln Ala Cys Trp Asp Trp 
        275                 280                 285             


Trp Gly Phe Ala Glu Ser Thr Trp Gln Ile Asn Pro Leu His Asp Thr 
    290                 295                 300                 


Arg Asp Gly Thr Gln Thr Gln Ala Leu Met Ala Met Leu Asp His Leu 
305                 310                 315                 320 


Gln Ser Ala Thr Ala Asn Lys Ala Ala Thr Ala Glu 
                325                 330         


<210> 9
<211> 332
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 9
Met Glu Glu Glu Ala Pro Gly Leu Pro Ala Leu Pro Tyr Ala Asn Asp 
1               5                   10                  15      


Gln Leu Tyr Val Val Gly Val Ser Ser Gly Gly Tyr Met Ala Leu Gln 
            20                  25                  30          


Leu Leu Val Ala Trp Pro Glu Arg Phe Ser Gly Val Gly Met Leu Ala 
        35                  40                  45              


Ala Leu Pro Trp Gly Cys Ala Gln Gly Ala Leu Ser Leu Ala Leu Asn 
    50                  55                  60                  


Gln Cys Met Met Thr Arg Arg Gly Leu Pro Ser Leu Asp Glu Leu Glu 
65                  70                  75                  80  


Gln Arg Arg Glu Arg Tyr Leu Ser Leu Asp Gln Val Gly Ser Gln Asp 
                85                  90                  95      


Ala Leu Ser Gln Leu Arg Ile Phe Val Trp His Gly Asp Ala Asp Glu 
            100                 105                 110         


Thr Val Ser Pro Ala Leu Leu Asp Leu Leu Ala Gln Gln Trp Gln Gly 
        115                 120                 125             


Trp Leu Glu Ser Pro Glu Gln Gln Leu Arg Tyr Val Gln Arg Ala Asn 
    130                 135                 140                 


Thr Gly His Gly Trp Pro Val Ala Met Pro Lys Asp Ala Pro Ile Asp 
145                 150                 155                 160 


Pro Gln Ser Leu Gly Asp Cys Arg Asn Gly Gly Gly Ser His Val Leu 
                165                 170                 175     


Ala Cys Gly Glu Asp Val Ala Trp Glu Met Met Ala Trp Leu Tyr Pro 
            180                 185                 190         


Glu Arg Glu Thr Asn Ala Ser Glu Gly Glu Leu Leu Ala Phe Asp Gln 
        195                 200                 205             


Ser Asp Phe Ala Ala Lys Gly Phe Ala Asp Thr Gly Tyr Val Phe Val 
    210                 215                 220                 


Pro Glu Ala Cys Glu Ala Gly Gly Cys Pro Val Thr Val Trp Leu His 
225                 230                 235                 240 


Gly Cys Gln Met Asn Ala Glu Ala Ile Asp Asp Thr Phe Val Arg Tyr 
                245                 250                 255     


Ser Gly Leu Asn Arg Trp Ala Ala Glu His Gly Gln Val Val Leu Tyr 
            260                 265                 270         


Pro Gln Ala Glu Ser Ser Met Ala Asn Pro Gln Ala Cys Trp Asp Trp 
        275                 280                 285             


Trp Gly Phe Ala Glu Ser Thr Trp Gln Ile Asn Pro Leu His Asp Thr 
    290                 295                 300                 


Arg Asp Gly Thr Gln Thr Gln Ala Leu Met Ala Met Leu Asp His Leu 
305                 310                 315                 320 


Gln Ser Ala Thr Ala Asn Lys Ala Ala Thr Ala Glu 
                325                 330         


<210> 10
<211> 332
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 10
Met Glu Glu Glu Ala Pro Gly Leu Pro Ala Leu Pro Tyr Ala Asn Asp 
1               5                   10                  15      


Gln Leu Tyr Val Val Gly Val Ser Ser Gly Gly Tyr Met Ala Leu Gln 
            20                  25                  30          


Leu Leu Val Ala Trp Pro Glu Arg Phe Ser Gly Val Gly Met Leu Ala 
        35                  40                  45              


Ala Leu Pro Trp Gly Cys Ala Gln Gly Ser Leu Ser Leu Ala Leu Asn 
    50                  55                  60                  


Gln Cys Met Met Thr Arg Arg Gly Leu Pro Ser Leu Asp Glu Leu Glu 
65                  70                  75                  80  


Gln Arg Arg Glu Arg Tyr Leu Ser Ser Asp Gln Val Gly Ser Gln Asp 
                85                  90                  95      


Ala Leu Ser Gln Leu Arg Ile Phe Val Trp His Gly Asp Ala Asp Glu 
            100                 105                 110         


Thr Val Ser Pro Ala Leu Leu Asp Leu Leu Ala Gln Gln Trp Gln Gly 
        115                 120                 125             


Trp Leu Glu Ser Pro Glu Gln Gln Leu Arg Tyr Val Gln Ser Ala Asn 
    130                 135                 140                 


Thr Gly His Gly Trp Pro Val Ala Met Pro Lys Asp Ala Pro Ser Asp 
145                 150                 155                 160 


Pro Gln Ser Leu Gly Asp Cys Arg Asn Gly Gly Gly Ser His Val Leu 
                165                 170                 175     


Ala Cys Gly Glu Asp Val Ala Trp Glu Met Met Ala Trp Leu Tyr Pro 
            180                 185                 190         


Glu Arg Glu Thr Asn Ala Ser Glu Gly Glu Leu Leu Ala Phe Asp Gln 
        195                 200                 205             


Ser Asp Phe Ala Ala Lys Gly Phe Ala Asp Thr Gly Tyr Val Phe Val 
    210                 215                 220                 


Pro Glu Ala Cys Glu Ala Gly Gly Cys Pro Val Thr Val Trp Leu His 
225                 230                 235                 240 


Gly Cys Gln Met Asn Ala Glu Ala Ile Asp Asp Thr Phe Val Arg Tyr 
                245                 250                 255     


Ser Gly Leu Asn Arg Trp Ala Ala Glu His Gly Gln Val Val Leu Tyr 
            260                 265                 270         


Pro Gln Ala Glu Ser Ser Met Ala Asn Pro Gln Ala Cys Trp Asp Trp 
        275                 280                 285             


Trp Gly Phe Ala Glu Ser Thr Trp Gln Ile Asn Pro Leu His Asp Thr 
    290                 295                 300                 


Arg Asp Gly Thr Gln Thr Gln Ala Leu Met Ala Met Leu Asp His Leu 
305                 310                 315                 320 


Gln Ser Ala Thr Ala Asn Lys Ala Ala Thr Ala Glu 
                325                 330         


<210> 11
<211> 332
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 11
Met Glu Glu Glu Ala Pro Gly Leu Pro Ala Leu Gly Ala Ala Phe Asp 
1               5                   10                  15      


Gln Ala Ser Val Val Gly Val Ser Ser Gly Gly Tyr Met Ala Ser Gln 
            20                  25                  30          


Leu Ala Val Ala Trp Pro Glu Arg Phe Ser Gly Val Gly Trp Leu Ala 
        35                  40                  45              


Gly Gly Pro Trp Gly Cys Ala Gln Gly Ala Leu Ser Leu Ala Leu Asn 
    50                  55                  60                  


Gln Cys Met Met Thr Arg Arg Gly Leu Pro Ser Leu Asp Glu Leu Leu 
65                  70                  75                  80  


Gln Arg Leu Glu Arg Tyr Leu Ser Leu Asp Gln Val Gly Ser Leu Asp 
                85                  90                  95      


Ala Leu Ser Gln Leu Arg Ala Phe Val Trp His Gly Asp Ala Asp Glu 
            100                 105                 110         


Thr Val Ser Pro Ala Leu Gly Asp Leu Leu Ala Gln Gln Trp Gln Gly 
        115                 120                 125             


Trp Leu Glu Ser Pro Glu Gln Gln Leu Arg Tyr Val Ile Arg Ala Asn 
    130                 135                 140                 


Thr Gly His Gly Trp Trp Val Ala Met Pro Lys Asp Ala Pro Ile Asp 
145                 150                 155                 160 


Pro Gln Ser Leu Gly Asp Cys Arg Asn Gly Gly Gly Ser His Val Leu 
                165                 170                 175     


Ala Cys Gly Glu Asp Val Ala Gly Glu Met Met Ala Trp Leu Tyr Pro 
            180                 185                 190         


Glu Arg Glu Thr Asn Ala Ser Glu Gly Glu Leu Leu Ala Phe Asp Gln 
        195                 200                 205             


Ser Asp Phe Ala Ala Lys Gly Phe Ala Asp Thr Gly Tyr Val Phe Val 
    210                 215                 220                 


Pro Glu Ala Cys Glu Ala Gly Gly Cys Pro Val Thr Val Ala Leu His 
225                 230                 235                 240 


Gly Cys Gln Met Asn Ala Glu Ala Ile Asp Asp Thr Phe Val Arg Tyr 
                245                 250                 255     


Ser Gly Leu Asn Arg Trp Ala Ala Glu His Gly Gln Val Val Leu Tyr 
            260                 265                 270         


Pro Gln Ala Glu Ser Ser Met Ala Asn Pro Gln Ala Cys Trp Asp Trp 
        275                 280                 285             


Trp Gly Phe Ala Glu Ser Thr Trp Gln Ile Asn Pro Leu His Asp Thr 
    290                 295                 300                 


Arg Asp Gly Thr Gln Thr Gln Ala Leu Met Ala Met Leu Asp His Leu 
305                 310                 315                 320 


Thr Ser Ala Thr Ala Asn Lys Ala Ala Thr Ala Glu 
                325                 330         


<210> 12
<211> 332
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 12
Met Glu Glu Glu Ala Pro Gly Leu Pro Ala Leu Gly Ala Ala Phe Asp 
1               5                   10                  15      


Gln Ala Ser Val Val Gly Val Ser Ser Gly Gly Tyr Met Ala Ser Gln 
            20                  25                  30          


Leu Ala Val Ala Trp Pro Glu Arg Phe Ser Gly Val Gly Trp Leu Ala 
        35                  40                  45              


Gly Gly Pro Trp Gly Cys Ala Gln Gly Ser Leu Ser Leu Ala Leu Asn 
    50                  55                  60                  


Gln Cys Met Met Thr Arg Arg Gly Leu Pro Ser Leu Asp Glu Leu Leu 
65                  70                  75                  80  


Gln Arg Leu Glu Arg Tyr Leu Ser Ser Asp Gln Val Gly Ser Leu Asp 
                85                  90                  95      


Ala Leu Ser Gln Leu Arg Ala Phe Val Trp His Gly Asp Ala Asp Glu 
            100                 105                 110         


Thr Val Ser Pro Ala Leu Gly Asp Leu Leu Ala Gln Gln Trp Gln Gly 
        115                 120                 125             


Trp Leu Glu Ser Pro Glu Gln Gln Leu Arg Tyr Val Ile Ser Ala Asn 
    130                 135                 140                 


Thr Gly His Gly Trp Trp Val Ala Met Pro Lys Asp Ala Pro Ser Asp 
145                 150                 155                 160 


Pro Gln Ser Leu Gly Asp Cys Arg Asn Gly Gly Gly Ser His Val Leu 
                165                 170                 175     


Ala Cys Gly Glu Asp Val Ala Gly Glu Met Met Ala Trp Leu Tyr Pro 
            180                 185                 190         


Glu Arg Glu Thr Asn Ala Ser Glu Gly Glu Leu Leu Ala Phe Asp Gln 
        195                 200                 205             


Ser Asp Phe Ala Ala Lys Gly Phe Ala Asp Thr Gly Tyr Val Phe Val 
    210                 215                 220                 


Pro Glu Ala Cys Glu Ala Gly Gly Cys Pro Val Thr Val Ala Leu His 
225                 230                 235                 240 


Gly Cys Gln Met Asn Ala Glu Ala Ile Asp Asp Thr Phe Val Arg Tyr 
                245                 250                 255     


Ser Gly Leu Asn Arg Trp Ala Ala Glu His Gly Gln Val Val Leu Tyr 
            260                 265                 270         


Pro Gln Ala Glu Ser Ser Met Ala Asn Pro Gln Ala Cys Trp Asp Trp 
        275                 280                 285             


Trp Gly Phe Ala Glu Ser Thr Trp Gln Ile Asn Pro Leu His Asp Thr 
    290                 295                 300                 


Arg Asp Gly Thr Gln Thr Gln Ala Leu Met Ala Met Leu Asp His Leu 
305                 310                 315                 320 


Thr Ser Ala Thr Ala Asn Lys Ala Ala Thr Ala Glu 
                325                 330         


<210> 13
<211> 332
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 13
Met Glu Glu Glu Ala Pro Gly Leu Pro Ala Leu Pro Tyr Ala Phe Asp 
1               5                   10                  15      


Gln Leu Tyr Val Val Gly Val Ser Ser Gly Gly Tyr Met Ala Leu Gln 
            20                  25                  30          


Leu Leu Val Ala Trp Pro Glu Arg Phe Ser Gly Val Gly Trp Leu Ala 
        35                  40                  45              


Gly Leu Pro Trp Gly Cys Ala Gln Gly Ala Leu Ser Leu Ala Leu Asn 
    50                  55                  60                  


Gln Cys Met Met Thr Arg Arg Gly Leu Pro Ser Leu Asp Glu Leu Leu 
65                  70                  75                  80  


Gln Arg Leu Glu Arg Tyr Leu Ser Leu Asp Gln Val Gly Ser Leu Asp 
                85                  90                  95      


Ala Leu Ser Gln Leu Arg Ile Phe Val Trp His Gly Asp Ala Asp Glu 
            100                 105                 110         


Thr Val Ser Pro Ala Leu Leu Asp Leu Leu Ala Gln Gln Trp Gln Gly 
        115                 120                 125             


Trp Leu Glu Ser Pro Glu Gln Gln Leu Arg Tyr Val Ile Arg Ala Asn 
    130                 135                 140                 


Thr Gly His Gly Trp Trp Val Ala Met Pro Lys Asp Ala Pro Ile Asp 
145                 150                 155                 160 


Pro Gln Ser Leu Gly Asp Cys Arg Asn Gly Gly Gly Ser His Val Leu 
                165                 170                 175     


Ala Cys Gly Glu Asp Val Ala Trp Glu Met Met Ala Trp Leu Tyr Pro 
            180                 185                 190         


Glu Arg Glu Thr Asn Ala Ser Glu Gly Glu Leu Leu Ala Phe Asp Gln 
        195                 200                 205             


Ser Asp Phe Ala Ala Lys Gly Phe Ala Asp Thr Gly Tyr Val Phe Val 
    210                 215                 220                 


Pro Glu Ala Cys Glu Ala Gly Gly Cys Pro Val Thr Val Trp Leu His 
225                 230                 235                 240 


Gly Cys Gln Met Asn Ala Glu Ala Ile Asp Asp Thr Phe Val Arg Tyr 
                245                 250                 255     


Ser Gly Leu Asn Arg Trp Ala Ala Glu His Gly Gln Val Val Leu Tyr 
            260                 265                 270         


Pro Gln Ala Glu Ser Ser Met Ala Asn Pro Gln Ala Cys Trp Asp Trp 
        275                 280                 285             


Trp Gly Phe Ala Glu Ser Thr Trp Gln Ile Asn Pro Leu His Asp Thr 
    290                 295                 300                 


Arg Asp Gly Thr Gln Thr Gln Ala Leu Met Ala Met Leu Asp His Leu 
305                 310                 315                 320 


Thr Ser Ala Thr Ala Asn Lys Ala Ala Thr Ala Glu 
                325                 330         


<210> 14
<211> 108
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 14
Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp Val 
1               5                   10                  15      


Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp Cys 
            20                  25                  30          


Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp Glu 
        35                  40                  45              


Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn Pro 
    50                  55                  60                  


Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu 
65                  70                  75                  80  


Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser Lys 
                85                  90                  95      


Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala 
            100                 105             


<210> 15
<211> 332
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 15
Met Glu Glu Glu Ala Pro Gly Leu Pro Ala Leu Pro Tyr Ala Phe Asp 
1               5                   10                  15      


Gln Leu Tyr Val Val Gly Val Ser Ser Gly Gly Tyr Met Ala Leu Gln 
            20                  25                  30          


Leu Leu Val Ala Trp Pro Glu Arg Phe Ser Gly Val Gly Trp Leu Ala 
        35                  40                  45              


Gly Leu Pro Trp Gly Cys Ala Gln Gly Ser Leu Ser Leu Ala Leu Asn 
    50                  55                  60                  


Gln Cys Met Met Thr Arg Arg Gly Leu Pro Ser Leu Asp Glu Leu Leu 
65                  70                  75                  80  


Gln Arg Leu Glu Arg Tyr Leu Ser Ser Asp Gln Val Gly Ser Leu Asp 
                85                  90                  95      


Ala Leu Ser Gln Leu Arg Ile Phe Val Trp His Gly Asp Ala Asp Glu 
            100                 105                 110         


Thr Val Ser Pro Ala Leu Leu Asp Leu Leu Ala Gln Gln Trp Gln Gly 
        115                 120                 125             


Trp Leu Glu Ser Pro Glu Gln Gln Leu Arg Tyr Val Ile Ser Ala Asn 
    130                 135                 140                 


Thr Gly His Gly Trp Trp Val Ala Met Pro Lys Asp Ala Pro Ser Asp 
145                 150                 155                 160 


Pro Gln Ser Leu Gly Asp Cys Arg Asn Gly Gly Gly Ser His Val Leu 
                165                 170                 175     


Ala Cys Gly Glu Asp Val Ala Trp Glu Met Met Ala Trp Leu Tyr Pro 
            180                 185                 190         


Glu Arg Glu Thr Asn Ala Ser Glu Gly Glu Leu Leu Ala Phe Asp Gln 
        195                 200                 205             


Ser Asp Phe Ala Ala Lys Gly Phe Ala Asp Thr Gly Tyr Val Phe Val 
    210                 215                 220                 


Pro Glu Ala Cys Glu Ala Gly Gly Cys Pro Val Thr Val Trp Leu His 
225                 230                 235                 240 


Gly Cys Gln Met Asn Ala Glu Ala Ile Asp Asp Thr Phe Val Arg Tyr 
                245                 250                 255     


Ser Gly Leu Asn Arg Trp Ala Ala Glu His Gly Gln Val Val Leu Tyr 
            260                 265                 270         


Pro Gln Ala Glu Ser Ser Met Ala Asn Pro Gln Ala Cys Trp Asp Trp 
        275                 280                 285             


Trp Gly Phe Ala Glu Ser Thr Trp Gln Ile Asn Pro Leu His Asp Thr 
    290                 295                 300                 


Arg Asp Gly Thr Gln Thr Gln Ala Leu Met Ala Met Leu Asp His Leu 
305                 310                 315                 320 


Thr Ser Ala Thr Ala Asn Lys Ala Ala Thr Ala Glu 
                325                 330         


<210> 16
<211> 332
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (12)..(12)
<223> P, L, F, W or N

<220>
<221> MOD_RES
<222> (13)..(13)
<223> Y, W, I, F, L or V

<220>
<221> MOD_RES
<222> (15)..(15)
<223> F, L or V

<220>
<221> MOD_RES
<222> (17)..(17)
<223> L, F, I, W, V, M, Y, C or T

<220>
<221> MOD_RES
<222> (18)..(18)
<223> Y, W or F

<220>
<221> MOD_RES
<222> (31)..(31)
<223> L, I, W, F, V, M or Y

<220>
<221> MOD_RES
<222> (34)..(34)
<223> L, F, I, W, M or H

<220>
<221> MOD_RES
<222> (49)..(49)
<223> G, L, F or W

<220>
<221> MOD_RES
<222> (50)..(50)
<223> L, F, W or L

<220>
<221> MOD_RES
<222> (80)..(80)
<223> L, W, V or M

<220>
<221> MOD_RES
<222> (83)..(83)
<223> L, F, I, V, T, M, A or W

<220>
<221> MOD_RES
<222> (95)..(95)
<223> L, I or W

<220>
<221> MOD_RES
<222> (103)..(103)
<223> I, Y, L, F, W, V or M

<220>
<221> MOD_RES
<222> (119)..(119)
<223> L, F, W, I, M, Y, T, V or C

<220>
<221> MOD_RES
<222> (141)..(141)
<223> I, Y, W, L, V or F

<220>
<221> MOD_RES
<222> (184)..(184)
<223> W, L or F

<220>
<221> MOD_RES
<222> (238)..(238)
<223> W, F or Y

<220>
<221> MOD_RES
<222> (321)..(321)
<223> T, V, I, L, F or M

<400> 16
Met Glu Glu Glu Ala Pro Gly Leu Pro Ala Leu Xaa Xaa Ala Xaa Asp 
1               5                   10                  15      


Xaa Xaa Ser Val Val Gly Val Ser Ser Gly Gly Tyr Met Ala Xaa Gln 
            20                  25                  30          


Leu Xaa Val Ala Trp Pro Glu Arg Phe Ser Gly Val Gly Trp Leu Ala 
        35                  40                  45              


Xaa Xaa Pro Trp Gly Cys Ala Gln Gly Ala Leu Ser Leu Ala Leu Asn 
    50                  55                  60                  


Gln Cys Met Met Thr Arg Arg Gly Leu Pro Ser Leu Asp Glu Leu Xaa 
65                  70                  75                  80  


Gln Arg Xaa Glu Arg Tyr Leu Ser Leu Asp Gln Val Gly Ser Xaa Asp 
                85                  90                  95      


Ala Leu Ser Gln Leu Arg Xaa Phe Val Trp His Gly Asp Ala Asp Glu 
            100                 105                 110         


Thr Val Ser Pro Ala Leu Xaa Asp Leu Leu Ala Gln Gln Trp Gln Gly 
        115                 120                 125             


Trp Leu Glu Ser Pro Glu Gln Gln Leu Arg Tyr Val Xaa Ile Ala Asn 
    130                 135                 140                 


Thr Gly His Gly Trp Trp Val Ala Met Pro Lys Asp Ala Pro Ile Asp 
145                 150                 155                 160 


Pro Gln Ser Leu Gly Asp Cys Arg Asn Gly Gly Gly Ser His Val Leu 
                165                 170                 175     


Ala Cys Gly Glu Asp Val Ala Xaa Glu Met Met Ala Trp Leu Tyr Pro 
            180                 185                 190         


Glu Arg Glu Thr Asn Ala Ser Glu Gly Glu Leu Leu Ala Phe Asp Gln 
        195                 200                 205             


Ser Asp Phe Ala Ala Lys Gly Phe Ala Asp Thr Gly Tyr Val Phe Val 
    210                 215                 220                 


Pro Glu Ala Cys Glu Ala Gly Gly Cys Pro Val Thr Val Xaa Leu His 
225                 230                 235                 240 


Gly Cys Gln Met Asn Ala Glu Ala Ile Asp Asp Thr Phe Val Arg Tyr 
                245                 250                 255     


Ser Gly Leu Asn Arg Trp Ala Ala Glu His Gly Gln Val Val Leu Tyr 
            260                 265                 270         


Pro Gln Ala Glu Ser Ser Met Ala Asn Pro Gln Ala Cys Trp Asp Trp 
        275                 280                 285             


Trp Gly Phe Ala Glu Ser Thr Trp Gln Ile Asn Pro Leu His Asp Thr 
    290                 295                 300                 


Arg Asp Gly Thr Gln Thr Gln Ala Leu Met Ala Met Leu Asp His Leu 
305                 310                 315                 320 


Xaa Ser Ala Thr Ala Asn Lys Ala Ala Thr Ala Glu 
                325                 330         


<210> 17
<211> 333
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (13)..(13)
<223> Y, W, I or F

<220>
<221> MOD_RES
<222> (18)..(18)
<223> L, F, I or W

<220>
<221> MOD_RES
<222> (19)..(19)
<223> Y, W or L

<220>
<221> MOD_RES
<222> (83)..(83)
<223> L, F, I or V

<220>
<221> MOD_RES
<222> (119)..(119)
<223> L or F

<220>
<221> MOD_RES
<222> (322)..(322)
<223> T, V, I or L

<400> 17
Met Glu Glu Glu Ala Pro Gly Leu Pro Ala Leu Gly Xaa Ala Asn Asp 
1               5                   10                  15      


Gln Xaa Xaa Val Val Gly Val Ser Ser Gly Gly Tyr Met Ala Leu Gln 
            20                  25                  30          


Leu Leu Val Ala Trp Pro Glu Arg Phe Ser Gly Val Gly Met Leu Ala 
        35                  40                  45              


Gly Gly Pro Trp Gly Cys Ala Gln Gly Ala Leu Ser Leu Ala Leu Asn 
    50                  55                  60                  


Gln Cys Met Met Thr Arg Arg Gly Leu Pro Ser Leu Asp Glu Leu Glu 
65                  70                  75                  80  


Gln Arg Xaa Glu Arg Tyr Leu Ser Leu Asp Gln Val Gly Ser Gln Asp 
                85                  90                  95      


Ala Leu Ser Gln Leu Arg Ile Phe Val Trp His Gly Asp Ala Asp Glu 
            100                 105                 110         


Thr Val Ser Pro Ala Leu Xaa Asp Leu Leu Ala Gln Gln Trp Gln Gly 
        115                 120                 125             


Trp Leu Glu Ser Pro Glu Gln Gln Leu Arg Tyr Val Ile Gln Arg Ala 
    130                 135                 140                 


Asn Thr Gly His Gly Trp Pro Val Ala Met Pro Lys Asp Ala Pro Ile 
145                 150                 155                 160 


Asp Pro Gln Ser Leu Gly Asp Cys Arg Asn Gly Gly Gly Ser His Val 
                165                 170                 175     


Leu Ala Cys Gly Glu Asp Val Ala Trp Glu Met Met Ala Trp Leu Tyr 
            180                 185                 190         


Pro Glu Arg Glu Thr Asn Ala Ser Glu Gly Glu Leu Leu Ala Phe Asp 
        195                 200                 205             


Gln Ser Asp Phe Ala Ala Lys Gly Phe Ala Asp Thr Gly Tyr Val Phe 
    210                 215                 220                 


Val Pro Glu Ala Cys Glu Ala Gly Gly Cys Pro Val Thr Val Ala Leu 
225                 230                 235                 240 


His Gly Cys Gln Met Asn Ala Glu Ala Ile Asp Asp Thr Phe Val Arg 
                245                 250                 255     


Tyr Ser Gly Leu Asn Arg Trp Ala Ala Glu His Gly Gln Val Val Leu 
            260                 265                 270         


Tyr Pro Gln Ala Glu Ser Ser Met Ala Asn Pro Gln Ala Cys Trp Asp 
        275                 280                 285             


Trp Trp Gly Phe Ala Glu Ser Thr Trp Gln Ile Asn Pro Leu His Asp 
    290                 295                 300                 


Thr Arg Asp Gly Thr Gln Thr Gln Ala Leu Met Ala Met Leu Asp His 
305                 310                 315                 320 


Leu Xaa Ser Ala Thr Ala Asn Lys Ala Ala Thr Ala Glu 
                325                 330             


<210> 18
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 18
His His His His His His 
1               5       


<210> 19
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 19
Gly Ser Gly Ser Gly Ser 
1               5       


