                         SEQUENCE LISTING

<110>  New England Biolabs, Inc.
 
<120>  DNASE I VARIANTS, COMPOSITIONS, METHODS, AND KITS

<130>  NEB-437

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  DNase I variant V7 AA sequence

<400>  1

Leu Lys Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Ala Ser Tyr Ile Val Arg Ile Leu Arg Arg Tyr 
            20                  25                  30          


Asp Ile Ala Leu Ile Gln Glu Val Arg Asp Ser His Leu Val Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Tyr Leu Asn Gln Asp Asp Pro Asn Thr Tyr His 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Asn Ser Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Leu Phe Arg Pro Asp Gln Val Ser Val Leu Asp Ser Tyr Gln 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Pro Cys Gly Asn Asp Thr Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Val Val Lys Phe Ser Ser Pro Ser Thr Lys Val Lys Glu Phe 
        115                 120                 125             


Ala Ile Val Pro Leu His Ala Ala Pro Ser Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Asp Ser Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp His Leu 
145                 150                 155                 160 


Glu Asp Val Met Leu Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Pro Thr Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Ser Thr His 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Ser Leu Leu Gln Ser Ala 
    210                 215                 220                 


Val Val Pro Gly Ser Ala Ala Pro Phe Asp Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Asn Glu Met Ala Leu Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Thr 
            260 


<210>  2
<211>  260
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Human DNase I

<400>  2

Leu Lys Ile Ala Ala Phe Asn Ile Gln Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Val Ser Tyr Ile Val Gln Ile Leu Ser Arg Tyr 
            20                  25                  30          


Asp Ile Ala Leu Val Gln Glu Val Arg Asp Ser His Leu Thr Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Asn Leu Asn Gln Asp Ala Pro Asp Thr Tyr His 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Asn Ser Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Val Tyr Arg Pro Asp Gln Val Ser Ala Val Asp Ser Tyr Tyr 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Pro Cys Gly Asn Asp Thr Phe Asn Arg Glu 
            100                 105                 110         


Pro Ala Ile Val Arg Phe Phe Ser Arg Phe Thr Glu Val Arg Glu Phe 
        115                 120                 125             


Ala Ile Val Pro Leu His Ala Ala Pro Gly Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Asp Ala Leu Tyr Asp Val Tyr Leu Asp Val Gln Glu Lys Trp Gly Leu 
145                 150                 155                 160 


Glu Asp Val Met Leu Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val 
                165                 170                 175     


Arg Pro Ser Gln Trp Ser Ser Ile Arg Leu Trp Thr Ser Pro Thr Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Pro Thr His 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Met Leu Leu Arg Gly Ala 
    210                 215                 220                 


Val Val Pro Asp Ser Ala Leu Pro Phe Asn Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Asp Gln Leu Ala Gln Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Met Leu Lys 
            260 


<210>  3
<211>  260
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  Bovine DNase I

<400>  3

Leu Lys Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Ala Ser Tyr Ile Val Arg Ile Val Arg Arg Tyr 
            20                  25                  30          


Asp Ile Val Leu Ile Gln Glu Val Arg Asp Ser His Leu Val Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Tyr Leu Asn Gln Asp Asp Pro Asn Thr Tyr His 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Asn Ser Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Leu Phe Arg Pro Asn Lys Val Ser Val Leu Asp Thr Tyr Gln 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Ser Cys Gly Asn Asp Ser Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Val Val Lys Phe Ser Ser His Ser Thr Lys Val Lys Glu Phe 
        115                 120                 125             


Ala Ile Val Ala Leu His Ser Ala Pro Ser Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Asn Ser Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp His Leu 
145                 150                 155                 160 


Asn Asp Val Met Leu Met Gly Asp Phe Asn Ala Asp Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Ser Thr Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Ser Thr Asn 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Ser Leu Leu Gln Ser Ser 
    210                 215                 220                 


Val Val Pro Gly Ser Ala Ala Pro Phe Asp Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Asn Glu Met Ala Leu Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Thr 
            260 


<210>  4
<211>  816
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  His-tagged DNase I variant V7 AA sequence

<220>
<221>  misc_feature
<222>  (781)..(816)
<223>  C-terminal His tag

<400>  4
cttaaaatcg ccgcgtttaa tattcgcacc tttggggaga ccaaaatgag caacgccaca       60

cttgcttcct atatcgtccg tatcttgcgc cgctatgaca tcgctctgat ccaagaggtt      120

cgcgataaac acttagtggc agtgggtaag ttactggact acctgaacca ggatgaccct      180

aatacttacc attacgtagt gtctgaacct ctgggtcgca actcttacaa agaacgctac      240

ctgtttttgt tccgtccgga ccaagtatcc gtcctggatt cataccaata cgacgatggt      300

tgtgagccct gcgggaacga cacgtttagt cgtgaacctg cggtggtgaa attttcgagt      360

ccttcaacaa aagtcaaaga atttgcgatt gtccctcttc acgcagcccc atcagacgct      420

gttgcggaga ttgactcact ttacgatgtc taccttgatg tccaacagaa gtggcatttg      480

gaggacgtta tgcttatggg agattttaat gcaggatgct cgtatgttac ttcatcgcag      540

tggtcgtcta tccgccttcg cacctcccct actttccagt ggctgatccc cgacagtgcc      600

gacacgactg ccaccaaaac tcactgtgca tacgatcgca ttgttgttgc tgggagtctt      660

ttacaaagtg cggttgtccc aggcagcgcc gcaccgttcg acttccaagc tgcctatggt      720

ttaagcaacg aaatggcgtt ggctatctct gaccattatc cagtagaagt cacgctgact      780

aatagcgccg tcgaccatca ccatcaccat cactga                                816


<210>  5
<211>  271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  His-tagged DNase I variant V7 AA sequence

<220>
<221>  MISC_FEATURE
<222>  (261)..(271)
<223>  C-terminal His tag

<400>  5

Leu Lys Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Ala Ser Tyr Ile Val Arg Ile Leu Arg Arg Tyr 
            20                  25                  30          


Asp Ile Ala Leu Ile Gln Glu Val Arg Asp Lys His Leu Val Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Tyr Leu Asn Gln Asp Asp Pro Asn Thr Tyr His 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Asn Ser Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Leu Phe Arg Pro Asp Gln Val Ser Val Leu Asp Ser Tyr Gln 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Pro Cys Gly Asn Asp Thr Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Val Val Lys Phe Ser Ser Pro Ser Thr Lys Val Lys Glu Phe 
        115                 120                 125             


Ala Ile Val Pro Leu His Ala Ala Pro Ser Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Asp Ser Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp His Leu 
145                 150                 155                 160 


Glu Asp Val Met Leu Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Pro Thr Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Lys Thr His 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Ser Leu Leu Gln Ser Ala 
    210                 215                 220                 


Val Val Pro Gly Ser Ala Ala Pro Phe Asp Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Asn Glu Met Ala Leu Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Thr Asn Ser Ala Val Asp His His His His His His 
            260                 265                 270     


<210>  6
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  DNase I variant V7 AA sequence

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  x is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  x is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  x is any amino acid other than valine

<220>
<221>  MISC_FEATURE
<222>  (35)..(35)
<223>  x is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (43)..(43)
<223>  x is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (44)..(44)
<223>  x is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (74)..(74)
<223>  x is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (75)..(75)
<223>  x is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (87)..(87)
<223>  x is any amino acid other than asparagine

<220>
<221>  MISC_FEATURE
<222>  (88)..(88)
<223>  x is any amino acid other than lysine

<220>
<221>  MISC_FEATURE
<222>  (94)..(94)
<223>  x is any amino acid other than threonine

<220>
<221>  MISC_FEATURE
<222>  (103)..(103)
<223>  x is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (106)..(106)
<223>  x is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (108)..(108)
<223>  x is any amino acid other than serine

<220>
<221>  MISC_FEATURE
<222>  (121)..(121)
<223>  x is any amino acid other than histidine

<220>
<221>  MISC_FEATURE
<222>  (132)..(132)
<223>  x is any amino acid other than alanine

<220>
<221>  MISC_FEATURE
<222>  (135)..(135)
<223>  x is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  x is any amino acid other than asparagine

<220>
<221>  MISC_FEATURE
<222>  (161)..(161)
<223>  x is any amino acid other than asparagine

<220>
<221>  MISC_FEATURE
<222>  (172)..(172)
<223>  x is any amino acid other than aspartate

<220>
<221>  MISC_FEATURE
<222>  (190)..(190)
<223>  x is any amino acid other than serine

<220>
<221>  MISC_FEATURE
<222>  (205)..(205)
<223>  x is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (206)..(206)
<223>  x is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (207)..(207)
<223>  x is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (208)..(208)
<223>  x is any amino acid other than asparagine

<220>
<221>  MISC_FEATURE
<222>  (224)..(224)
<223>  x is any amino acid other than serine

<400>  6

Leu Lys Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Xaa Thr Lys Met 
1               5                   10                  15      


Ser Xaa Ala Thr Leu Ala Ser Tyr Ile Val Arg Ile Xaa Arg Arg Tyr 
            20                  25                  30          


Asp Ile Xaa Leu Ile Gln Glu Val Arg Asp Xaa Xaa Leu Val Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Tyr Leu Asn Gln Asp Asp Pro Asn Thr Tyr His 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Xaa Xaa Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Leu Phe Arg Pro Xaa Xaa Val Ser Val Leu Asp Xaa Tyr Gln 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Xaa Cys Gly Xaa Asp Xaa Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Val Val Lys Phe Ser Ser Xaa Ser Thr Lys Val Lys Glu Phe 
        115                 120                 125             


Ala Ile Val Xaa Leu His Xaa Ala Pro Ser Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Xaa Ser Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp His Leu 
145                 150                 155                 160 


Xaa Asp Val Met Leu Met Gly Asp Phe Asn Ala Xaa Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Xaa Thr Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Xaa Xaa Xaa Xaa 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Ser Leu Leu Gln Ser Xaa 
    210                 215                 220                 


Val Val Pro Gly Ser Ala Ala Pro Phe Asp Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Asn Glu Met Ala Leu Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Thr 
            260 


<210>  7
<211>  363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  DNase I variant V7 AA sequence with dual tag

<220>
<221>  MISC_FEATURE
<222>  (1)..(92)
<223>  N-terminal chitin binding protein tag plus linker

<220>
<221>  MISC_FEATURE
<222>  (353)..(363)
<223>  C-terminal His tag

<400>  7

Asp Ser Trp Ala Val Thr Arg Ala Lys Glu Leu Asn Glu Gln Phe Val 
1               5                   10                  15      


Lys Gly Glu Leu Asn Gly Lys Asp Ser Cys Ser Asp Gly Glu Ile Ser 
            20                  25                  30          


Cys Thr Ala Asp Gly Lys Ile Ala Ile Cys Asn Tyr Gly Ala Trp Val 
        35                  40                  45              


Tyr Thr Glu Cys Ala Ala Gly Thr Thr Cys Phe Ala Tyr Asp Ser Gly 
    50                  55                  60                  


Asp Ser Val Tyr Thr Ser Cys Asn Phe Thr Tyr Leu Lys Pro Asp Val 
65                  70                  75                  80  


Val Phe Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Lys Ile Ala 
                85                  90                  95      


Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met Ser Asn Ala Thr 
            100                 105                 110         


Leu Ala Ser Tyr Ile Val Arg Ile Leu Arg Arg Tyr Asp Ile Ala Leu 
        115                 120                 125             


Ile Gln Glu Val Arg Asp Lys His Leu Val Ala Val Gly Lys Leu Leu 
    130                 135                 140                 


Asp Tyr Leu Asn Gln Asp Asp Pro Asn Thr Tyr His Tyr Val Val Ser 
145                 150                 155                 160 


Glu Pro Leu Gly Arg Asn Ser Tyr Lys Glu Arg Tyr Leu Phe Leu Phe 
                165                 170                 175     


Arg Pro Asp Gln Val Ser Val Leu Asp Ser Tyr Gln Tyr Asp Asp Gly 
            180                 185                 190         


Cys Glu Pro Cys Gly Asn Asp Thr Phe Ser Arg Glu Pro Ala Val Val 
        195                 200                 205             


Lys Phe Ser Ser Pro Ser Thr Lys Val Lys Glu Phe Ala Ile Val Pro 
    210                 215                 220                 


Leu His Ala Ala Pro Ser Asp Ala Val Ala Glu Ile Asp Ser Leu Tyr 
225                 230                 235                 240 


Asp Val Tyr Leu Asp Val Gln Gln Lys Trp His Leu Glu Asp Val Met 
                245                 250                 255     


Leu Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val Thr Ser Ser Gln 
            260                 265                 270         


Trp Ser Ser Ile Arg Leu Arg Thr Ser Pro Thr Phe Gln Trp Leu Ile 
        275                 280                 285             


Pro Asp Ser Ala Asp Thr Thr Ala Thr Lys Thr His Cys Ala Tyr Asp 
    290                 295                 300                 


Arg Ile Val Val Ala Gly Ser Leu Leu Gln Ser Ala Val Val Pro Gly 
305                 310                 315                 320 


Ser Ala Ala Pro Phe Asp Phe Gln Ala Ala Tyr Gly Leu Ser Asn Glu 
                325                 330                 335     


Met Ala Leu Ala Ile Ser Asp His Tyr Pro Val Glu Val Thr Leu Thr 
            340                 345                 350         


Asn Ser Ala Val Asp His His His His His His 
        355                 360             


<210>  8
<211>  360
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  DNase I variant V7 AA sequence with a dual tag

<220>
<221>  MISC_FEATURE
<222>  (1)..(89)
<223>  N-terminal alpha mating factor

<220>
<221>  MISC_FEATURE
<222>  (350)..(360)
<223>  C-terminal His tag

<400>  8

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser Leu Glu Lys Arg Glu Ala Glu Ala Leu Lys Ile Ala Ala Phe Asn 
                85                  90                  95      


Ile Arg Thr Phe Gly Glu Thr Lys Met Ser Asn Ala Thr Leu Ala Ser 
            100                 105                 110         


Tyr Ile Val Arg Ile Leu Arg Arg Tyr Asp Ile Ala Leu Ile Gln Glu 
        115                 120                 125             


Val Arg Asp Ser His Leu Val Ala Val Gly Lys Leu Leu Asp Tyr Leu 
    130                 135                 140                 


Asn Gln Asp Asp Pro Asn Thr Tyr His Tyr Val Val Ser Glu Pro Leu 
145                 150                 155                 160 


Gly Arg Asn Ser Tyr Lys Glu Arg Tyr Leu Phe Leu Phe Arg Pro Asp 
                165                 170                 175     


Gln Val Ser Val Leu Asp Ser Tyr Gln Tyr Asp Asp Gly Cys Glu Pro 
            180                 185                 190         


Cys Gly Asn Asp Thr Phe Ser Arg Glu Pro Ala Val Val Lys Phe Ser 
        195                 200                 205             


Ser Pro Ser Thr Lys Val Lys Glu Phe Ala Ile Val Pro Leu His Ala 
    210                 215                 220                 


Ala Pro Ser Asp Ala Val Ala Glu Ile Asp Ser Leu Tyr Asp Val Tyr 
225                 230                 235                 240 


Leu Asp Val Gln Gln Lys Trp His Leu Glu Asp Val Met Leu Met Gly 
                245                 250                 255     


Asp Phe Asn Ala Gly Cys Ser Tyr Val Thr Ser Ser Gln Trp Ser Ser 
            260                 265                 270         


Ile Arg Leu Arg Thr Ser Pro Thr Phe Gln Trp Leu Ile Pro Asp Ser 
        275                 280                 285             


Ala Asp Thr Thr Ala Thr Ser Thr His Cys Ala Tyr Asp Arg Ile Val 
    290                 295                 300                 


Val Ala Gly Ser Leu Leu Gln Ser Ala Val Val Pro Gly Ser Ala Ala 
305                 310                 315                 320 


Pro Phe Asp Phe Gln Ala Ala Tyr Gly Leu Ser Asn Glu Met Ala Leu 
                325                 330                 335     


Ala Ile Ser Asp His Tyr Pro Val Glu Val Thr Leu Thr Asn Ser Ala 
            340                 345                 350         


Val Asp His His His His His His 
        355                 360 


<210>  9
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  DNase I variant V7+ AA sequence

<400>  9

Leu Lys Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Ser Ser Tyr Ile Val Arg Ile Leu Ser Arg Tyr 
            20                  25                  30          


Asp Ile Ala Leu Ile Gln Glu Val Arg Asp Ser His Leu Thr Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Tyr Leu Asn Gln Asp Asp Pro Asn Thr Tyr His 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Asn Ser Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Leu Phe Arg Pro Asp Gln Val Ser Val Leu Asp Ser Tyr Gln 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Pro Cys Gly Asn Asp Thr Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Val Val Lys Phe Ser Ser Pro Ser Thr Lys Val Lys Glu Phe 
        115                 120                 125             


Ala Ile Val Pro Leu His Ala Ala Pro Ser Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Asp Ser Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp His Leu 
145                 150                 155                 160 


Glu Asp Ile Met Leu Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Pro Thr Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Ser Thr His 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Ser Leu Leu Gln Ser Ala 
    210                 215                 220                 


Val Val Pro Gly Ser Ala Ala Pro Phe Asp Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Asn Glu Met Ala Leu Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Thr 
            260 


<210>  10
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  DNase I variant V7+ AA sequence

<220>
<221>  MISC_FEATURE
<222>  (22)..(22)
<223>  x is any amino acid other than alanine

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  x is any amino acid other than valine

<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  x is any amino acid other than arginine

<220>
<221>  MISC_FEATURE
<222>  (35)..(35)
<223>  x is any amino acid other than valine

<220>
<221>  MISC_FEATURE
<222>  (46)..(46)
<223>  x is any amino acid other than valine

<220>
<221>  MISC_FEATURE
<222>  (87)..(87)
<223>  x is any amino acid other than asparagine

<220>
<221>  MISC_FEATURE
<222>  (88)..(88)
<223>  x is any amino acid other than lysine

<220>
<221>  MISC_FEATURE
<222>  (94)..(94)
<223>  x is any amino acid other than threonine

<220>
<221>  MISC_FEATURE
<222>  (103)..(103)
<223>  x is any amino acid other than serine

<220>
<221>  MISC_FEATURE
<222>  (108)..(108)
<223>  x is any amino acid other than serine

<220>
<221>  MISC_FEATURE
<222>  (121)..(121)
<223>  x is any amino acid other than histidine

<220>
<221>  MISC_FEATURE
<222>  (132)..(132)
<223>  x is any amino acid other than alanine

<220>
<221>  MISC_FEATURE
<222>  (135)..(135)
<223>  x is any amino acid other than serine

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  x is any amino acid other than asparagine

<220>
<221>  MISC_FEATURE
<222>  (161)..(161)
<223>  x is any amino acid other than asparagine

<220>
<221>  MISC_FEATURE
<222>  (163)..(163)
<223>  x is any amino acid other than valine

<220>
<221>  MISC_FEATURE
<222>  (172)..(172)
<223>  x is any amino acid other than aspartate

<220>
<221>  MISC_FEATURE
<222>  (190)..(190)
<223>  x is any amino acid other than serine

<220>
<221>  MISC_FEATURE
<222>  (208)..(208)
<223>  x is any amino acid other than asparagine

<220>
<221>  MISC_FEATURE
<222>  (224)..(224)
<223>  x is any amino acid other than serine

<400>  10

Leu Lys Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Xaa Ser Tyr Ile Val Arg Ile Xaa Xaa Arg Tyr 
            20                  25                  30          


Asp Ile Xaa Leu Ile Gln Glu Val Arg Asp Ser His Leu Xaa Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Tyr Leu Asn Gln Asp Asp Pro Asn Thr Tyr His 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Asn Ser Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Leu Phe Arg Pro Xaa Xaa Val Ser Val Leu Asp Xaa Tyr Gln 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Xaa Cys Gly Asn Asp Xaa Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Val Val Lys Phe Ser Ser Xaa Ser Thr Lys Val Lys Glu Phe 
        115                 120                 125             


Ala Ile Val Xaa Leu His Xaa Ala Pro Ser Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Xaa Ser Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp His Leu 
145                 150                 155                 160 


Xaa Asp Xaa Met Leu Met Gly Asp Phe Asn Ala Xaa Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Xaa Thr Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Ser Thr Xaa 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Ser Leu Leu Gln Ser Xaa 
    210                 215                 220                 


Val Val Pro Gly Ser Ala Ala Pro Phe Asp Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Asn Glu Met Ala Leu Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Thr 
            260 


<210>  11
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synnthetic construct


<220>
<221>  misc_feature
<223>  DNase I variant AA sequence

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  x is any amino acid

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  x is any amino acid

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  x is any amino acid other than alanine

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  x is any amino acid other than valine

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  x is any amino acid other than arginine

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  x is any amino acid other than valine

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  x is any amino acid

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  x is any amino acid

<220>
<221>  misc_feature
<222>  (46)..(46)
<223>  x is any amino acid other than valine

<220>
<221>  misc_feature
<222>  (74)..(74)
<223>  x is any amino acid

<220>
<221>  misc_feature
<222>  (75)..(75)
<223>  x is any amino acid

<220>
<221>  misc_feature
<222>  (87)..(87)
<223>  x is any amino acid other than asparagine

<220>
<221>  misc_feature
<222>  (88)..(88)
<223>  x is any amino acid other than lysine

<220>
<221>  misc_feature
<222>  (94)..(94)
<223>  x is any amino acid other than theronine

<220>
<221>  misc_feature
<222>  (103)..(103)
<223>  x is any amino acid

<220>
<221>  misc_feature
<222>  (106)..(106)
<223>  x is any amino acid

<220>
<221>  misc_feature
<222>  (108)..(108)
<223>  x is any amino acid other than serine

<220>
<221>  misc_feature
<222>  (121)..(121)
<223>  x is any amino acid other than histidine

<220>
<221>  misc_feature
<222>  (132)..(132)
<223>  x is any amino acid other than alanine

<220>
<221>  misc_feature
<222>  (135)..(135)
<223>  x is any amino acid other than serine

<220>
<221>  misc_feature
<222>  (145)..(145)
<223>  x is any amino acid other than asparagine

<220>
<221>  misc_feature
<222>  (161)..(161)
<223>  x is any amino acid other than asparagine

<220>
<221>  misc_feature
<222>  (163)..(163)
<223>  x is any amino acid other than valine

<220>
<221>  misc_feature
<222>  (172)..(172)
<223>  x is any amino acid other than aspartate

<220>
<221>  misc_feature
<222>  (190)..(190)
<223>  x is any amino acid other than serine

<220>
<221>  misc_feature
<222>  (205)..(205)
<223>  x is any amino acid

<220>
<221>  misc_feature
<222>  (206)..(206)
<223>  x is any amino acid

<220>
<221>  misc_feature
<222>  (207)..(207)
<223>  x is any amino acid

<220>
<221>  misc_feature
<222>  (208)..(208)
<223>  x is any amino acid other than asparagine

<220>
<221>  misc_feature
<222>  (224)..(224)
<223>  x is any amino acid other than serine

<400>  11

Leu Lys Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Xaa Thr Lys Met 
1               5                   10                  15      


Ser Xaa Ala Thr Leu Xaa Ser Tyr Ile Val Arg Ile Xaa Xaa Arg Tyr 
            20                  25                  30          


Asp Ile Xaa Leu Ile Gln Glu Val Arg Asp Xaa Xaa Leu Xaa Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Tyr Leu Asn Gln Asp Asp Pro Asn Thr Tyr His 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Xaa Xaa Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Leu Phe Arg Pro Xaa Xaa Val Ser Val Leu Asp Xaa Tyr Gln 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Xaa Cys Gly Xaa Asp Xaa Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Val Val Lys Phe Ser Ser Xaa Ser Thr Lys Val Lys Glu Phe 
        115                 120                 125             


Ala Ile Val Xaa Leu His Xaa Ala Pro Ser Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Xaa Ser Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp His Leu 
145                 150                 155                 160 


Xaa Asp Xaa Met Leu Met Gly Asp Phe Asn Ala Xaa Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Xaa Thr Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Xaa Xaa Xaa Xaa 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Ser Leu Leu Gln Ser Xaa 
    210                 215                 220                 


Val Val Pro Gly Ser Ala Ala Pro Phe Asp Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Asn Glu Met Ala Leu Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Thr 
            260 


<210>  12
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  12
tctaagccgt gtacattttt tgtacacggc ttaga                                  35


