               SEQUENCE LISTING

<110> EryDel S.p.A.

<120> Engineering new metabolic pathways in isolated cells for the degradation of guanidinoacetic acid and simultaneous production of creatine

<130> BW1174R

<150> IT102021000013814
<151> 2021-05-27

<160> 32

<170> BiSSAP 1.3.6

<210> 1
<211> 235
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQ ID NO 1 – Ammino acid sequence of GAMT wild-type without the
      initial methionine

<400> 1
Ser Ala Pro Ser Ala Thr Pro Ile Phe Ala Pro Gly Glu Asn Cys Ser 
1               5                   10                  15      
Pro Ala Trp Gly Ala Ala Pro Ala Ala Tyr Asp Ala Ala Asp Thr His 
            20                  25                  30          
Leu Arg Ile Leu Gly Lys Pro Val Met Glu Arg Trp Glu Thr Pro Tyr 
        35                  40                  45              
Met His Ala Leu Ala Ala Ala Ala Ser Ser Lys Gly Gly Arg Val Leu 
    50                  55                  60                  
Glu Val Gly Phe Gly Met Ala Ile Ala Ala Ser Lys Val Gln Glu Ala 
65                  70                  75                  80  
Pro Ile Asp Glu His Trp Ile Ile Glu Cys Asn Asp Gly Val Phe Gln 
                85                  90                  95      
Arg Leu Arg Asp Trp Ala Pro Arg Gln Thr His Lys Val Ile Pro Leu 
            100                 105                 110         
Lys Gly Leu Trp Glu Asp Val Ala Pro Thr Leu Pro Asp Gly His Phe 
        115                 120                 125             
Asp Gly Ile Leu Tyr Asp Thr Tyr Pro Leu Ser Glu Glu Thr Trp His 
    130                 135                 140                 
Thr His Gln Phe Asn Phe Ile Lys Asn His Ala Phe Arg Leu Leu Lys 
145                 150                 155                 160 
Pro Gly Gly Val Leu Thr Tyr Cys Asn Leu Thr Ser Trp Gly Glu Leu 
                165                 170                 175     
Met Lys Ser Lys Tyr Ser Asp Ile Thr Ile Met Phe Glu Glu Thr Gln 
            180                 185                 190         
Val Pro Ala Leu Leu Glu Ala Gly Phe Arg Arg Glu Asn Ile Arg Thr 
        195                 200                 205             
Glu Val Met Ala Leu Val Pro Pro Ala Asp Cys Arg Tyr Tyr Ala Phe 
    210                 215                 220                 
Pro Gln Met Ile Thr Pro Leu Val Thr Lys Gly 
225                 230                 235 

<210> 2
<211> 235
<212> PRT
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 2 – Ammino acid sequence of GAMT CL 32
      (A26N-L37M-I187Q-V215Q)

<400> 2
Ser Ala Pro Ser Ala Thr Pro Ile Phe Ala Pro Gly Glu Asn Cys Ser 
1               5                   10                  15      
Pro Ala Trp Gly Ala Ala Pro Ala Asn Tyr Asp Ala Ala Asp Thr His 
            20                  25                  30          
Leu Arg Ile Met Gly Lys Pro Val Met Glu Arg Trp Glu Thr Pro Tyr 
        35                  40                  45              
Met His Ala Leu Ala Ala Ala Ala Ser Ser Lys Gly Gly Arg Val Leu 
    50                  55                  60                  
Glu Val Gly Phe Gly Met Ala Ile Ala Ala Ser Lys Val Gln Glu Ala 
65                  70                  75                  80  
Pro Ile Asp Glu His Trp Ile Ile Glu Cys Asn Asp Gly Val Phe Gln 
                85                  90                  95      
Arg Leu Arg Asp Trp Ala Pro Arg Gln Thr His Lys Val Ile Pro Leu 
            100                 105                 110         
Lys Gly Leu Trp Glu Asp Val Ala Pro Thr Leu Pro Asp Gly His Phe 
        115                 120                 125             
Asp Gly Ile Leu Tyr Asp Thr Tyr Pro Leu Ser Glu Glu Thr Trp His 
    130                 135                 140                 
Thr His Gln Phe Asn Phe Ile Lys Asn His Ala Phe Arg Leu Leu Lys 
145                 150                 155                 160 
Pro Gly Gly Val Leu Thr Tyr Cys Asn Leu Thr Ser Trp Gly Glu Leu 
                165                 170                 175     
Met Lys Ser Lys Tyr Ser Asp Ile Thr Gln Met Phe Glu Glu Thr Gln 
            180                 185                 190         
Val Pro Ala Leu Leu Glu Ala Gly Phe Arg Arg Glu Asn Ile Arg Thr 
        195                 200                 205             
Glu Val Met Ala Leu Gln Pro Pro Ala Asp Cys Arg Tyr Tyr Ala Phe 
    210                 215                 220                 
Pro Gln Met Ile Thr Pro Leu Val Thr Lys Gly 
225                 230                 235 

<210> 3
<211> 235
<212> PRT
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 3 – Ammino acid sequence of GAMT CL 51
      (L37M-I187Q-V215Q)

<400> 3
Ser Ala Pro Ser Ala Thr Pro Ile Phe Ala Pro Gly Glu Asn Cys Ser 
1               5                   10                  15      
Pro Ala Trp Gly Ala Ala Pro Ala Ala Tyr Asp Ala Ala Asp Thr His 
            20                  25                  30          
Leu Arg Ile Met Gly Lys Pro Val Met Glu Arg Trp Glu Thr Pro Tyr 
        35                  40                  45              
Met His Ala Leu Ala Ala Ala Ala Ser Ser Lys Gly Gly Arg Val Leu 
    50                  55                  60                  
Glu Val Gly Phe Gly Met Ala Ile Ala Ala Ser Lys Val Gln Glu Ala 
65                  70                  75                  80  
Pro Ile Asp Glu His Trp Ile Ile Glu Cys Asn Asp Gly Val Phe Gln 
                85                  90                  95      
Arg Leu Arg Asp Trp Ala Pro Arg Gln Thr His Lys Val Ile Pro Leu 
            100                 105                 110         
Lys Gly Leu Trp Glu Asp Val Ala Pro Thr Leu Pro Asp Gly His Phe 
        115                 120                 125             
Asp Gly Ile Leu Tyr Asp Thr Tyr Pro Leu Ser Glu Glu Thr Trp His 
    130                 135                 140                 
Thr His Gln Phe Asn Phe Ile Lys Asn His Ala Phe Arg Leu Leu Lys 
145                 150                 155                 160 
Pro Gly Gly Val Leu Thr Tyr Cys Asn Leu Thr Ser Trp Gly Glu Leu 
                165                 170                 175     
Met Lys Ser Lys Tyr Ser Asp Ile Thr Gln Met Phe Glu Glu Thr Gln 
            180                 185                 190         
Val Pro Ala Leu Leu Glu Ala Gly Phe Arg Arg Glu Asn Ile Arg Thr 
        195                 200                 205             
Glu Val Met Ala Leu Gln Pro Pro Ala Asp Cys Arg Tyr Tyr Ala Phe 
    210                 215                 220                 
Pro Gln Met Ile Thr Pro Leu Val Thr Lys Gly 
225                 230                 235 

<210> 4
<211> 235
<212> PRT
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 4 – Ammino acid sequence of GAMT CL 76
      (A26N-L37I-I187Q-V215Q)

<400> 4
Ser Ala Pro Ser Ala Thr Pro Ile Phe Ala Pro Gly Glu Asn Cys Ser 
1               5                   10                  15      
Pro Ala Trp Gly Ala Ala Pro Ala Asn Tyr Asp Ala Ala Asp Thr His 
            20                  25                  30          
Leu Arg Ile Ile Gly Lys Pro Val Met Glu Arg Trp Glu Thr Pro Tyr 
        35                  40                  45              
Met His Ala Leu Ala Ala Ala Ala Ser Ser Lys Gly Gly Arg Val Leu 
    50                  55                  60                  
Glu Val Gly Phe Gly Met Ala Ile Ala Ala Ser Lys Val Gln Glu Ala 
65                  70                  75                  80  
Pro Ile Asp Glu His Trp Ile Ile Glu Cys Asn Asp Gly Val Phe Gln 
                85                  90                  95      
Arg Leu Arg Asp Trp Ala Pro Arg Gln Thr His Lys Val Ile Pro Leu 
            100                 105                 110         
Lys Gly Leu Trp Glu Asp Val Ala Pro Thr Leu Pro Asp Gly His Phe 
        115                 120                 125             
Asp Gly Ile Leu Tyr Asp Thr Tyr Pro Leu Ser Glu Glu Thr Trp His 
    130                 135                 140                 
Thr His Gln Phe Asn Phe Ile Lys Asn His Ala Phe Arg Leu Leu Lys 
145                 150                 155                 160 
Pro Gly Gly Val Leu Thr Tyr Cys Asn Leu Thr Ser Trp Gly Glu Leu 
                165                 170                 175     
Met Lys Ser Lys Tyr Ser Asp Ile Thr Gln Met Phe Glu Glu Thr Gln 
            180                 185                 190         
Val Pro Ala Leu Leu Glu Ala Gly Phe Arg Arg Glu Asn Ile Arg Thr 
        195                 200                 205             
Glu Val Met Ala Leu Gln Pro Pro Ala Asp Cys Arg Tyr Tyr Ala Phe 
    210                 215                 220                 
Pro Gln Met Ile Thr Pro Leu Val Thr Lys Gly 
225                 230                 235 

<210> 5
<211> 395
<212> PRT
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 5 – Ammino acid sequence of MAT2A

<400> 5
Met Asn Gly Gln Leu Asn Gly Phe His Glu Ala Phe Ile Glu Glu Gly 
1               5                   10                  15      
Thr Phe Leu Phe Thr Ser Glu Ser Val Gly Glu Gly His Pro Asp Lys 
            20                  25                  30          
Ile Cys Asp Gln Ile Ser Asp Ala Val Leu Asp Ala His Leu Gln Gln 
        35                  40                  45              
Asp Pro Asp Ala Lys Val Ala Cys Glu Thr Val Ala Lys Thr Gly Met 
    50                  55                  60                  
Ile Leu Leu Ala Gly Glu Ile Thr Ser Arg Ala Ala Val Asp Tyr Gln 
65                  70                  75                  80  
Lys Val Val Arg Glu Ala Val Lys His Ile Gly Tyr Asp Asp Ser Ser 
                85                  90                  95      
Lys Gly Phe Asp Tyr Lys Thr Cys Asn Val Leu Val Ala Leu Glu Gln 
            100                 105                 110         
Gln Ser Pro Asp Ile Ala Gln Gly Val His Leu Asp Arg Asn Glu Glu 
        115                 120                 125             
Asp Ile Gly Ala Gly Asp Gln Gly Leu Met Phe Gly Tyr Ala Thr Asp 
    130                 135                 140                 
Glu Thr Glu Glu Cys Met Pro Leu Thr Ile Val Leu Ala His Lys Leu 
145                 150                 155                 160 
Asn Ala Lys Leu Ala Glu Leu Arg Arg Asn Gly Thr Leu Pro Trp Leu 
                165                 170                 175     
Arg Pro Asp Ser Lys Thr Gln Val Thr Val Gln Tyr Met Gln Asp Arg 
            180                 185                 190         
Gly Ala Val Leu Pro Ile Arg Val His Thr Ile Val Ile Ser Val Gln 
        195                 200                 205             
His Asp Glu Glu Val Cys Leu Asp Glu Met Arg Asp Ala Leu Lys Glu 
    210                 215                 220                 
Lys Val Ile Lys Ala Val Val Pro Ala Lys Tyr Leu Asp Glu Asp Thr 
225                 230                 235                 240 
Ile Tyr His Leu Gln Pro Ser Gly Arg Phe Val Ile Gly Gly Pro Gln 
                245                 250                 255     
Gly Asp Ala Gly Leu Thr Gly Arg Lys Ile Ile Val Asp Thr Tyr Gly 
            260                 265                 270         
Gly Trp Gly Ala His Gly Gly Gly Ala Phe Ser Gly Lys Asp Tyr Thr 
        275                 280                 285             
Lys Val Asp Arg Ser Ala Ala Tyr Ala Ala Arg Trp Val Ala Lys Ser 
    290                 295                 300                 
Leu Val Lys Gly Gly Leu Cys Arg Arg Val Leu Val Gln Val Ser Tyr 
305                 310                 315                 320 
Ala Ile Gly Val Ser His Pro Leu Ser Ile Ser Ile Phe His Tyr Gly 
                325                 330                 335     
Thr Ser Gln Lys Ser Glu Arg Glu Leu Leu Glu Ile Val Lys Lys Asn 
            340                 345                 350         
Phe Asp Leu Arg Pro Gly Val Ile Val Arg Asp Leu Asp Leu Lys Lys 
        355                 360                 365             
Pro Ile Tyr Gln Arg Thr Ala Ala Tyr Gly His Phe Gly Arg Asp Ser 
    370                 375                 380                 
Phe Pro Trp Glu Val Pro Lys Lys Leu Lys Tyr 
385                 390                 395 

<210> 6
<211> 963
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 6 – Nucleotidic sequence of His-UB-GAMT wild-type

<400> 6
atggcacatc accaccacca tcacgtgatg cagatcttcg tgaagaccct gactggtaag      60

accatcactc tcgaggtgga gccgagtgac accattgaga atgtcaaggc aaagatccaa     120

gacaaggaag gcatccctcc tgaccagcag aggttgatct ttgctgggaa acagctggaa     180

gatggacgca ccctgtctga ctacaacatc cagaaagagt ccaccctgca cctggtgctc     240

cgtctccgcg gtggatcggc ccccagcgcg acccccatct tcgcgcccgg cgagaactgc     300

agccccgcgt ggggggcggc gcccgcggcc tacgacgcag cggacacgca cctgcgcatc     360

ctgggcaagc cggtgatgga gcgctgggag accccctata tgcacgcgct ggccgccgcc     420

gcctcctcca aagggggccg ggtcctggag gtgggctttg gcatggccat cgcagcgtca     480

aaggtgcagg aggcgcccat tgatgagcat tggatcatcg agtgcaatga cggcgtcttc     540

cagcggctcc gggactgggc cccacggcag acacacaagg tcatcccctt gaaaggcctg     600

tgggaggatg tggcacccac cctgcctgac ggtcactttg atgggatcct gtacgacacg     660

tacccactct cggaggagac ctggcacaca caccagttca acttcatcaa gaaccacgcc     720

tttcgcctgc tgaagccggg gggcgtcctc acctactgca acctcacctc ctggggggag     780

ctgatgaagt ccaagtactc agacatcacc atcatgtttg aggagacgca ggtgcccgcg     840

ctgctggagg ccggcttccg gagggagaac atccgtacgg aggtgatggc gctggtccca     900

ccggccgact gccgctacta cgccttccca cagatgatca cgcccctggt gaccaaaggc     960

tga                                                                   963


<210> 7
<211> 963
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 7 – Nucleotidic sequence of His-UB-GAMT mutant CLONE 32
      (A26N-L37M-I187Q-V215Q)

<400> 7
atggcacatc accaccacca tcacgtgatg cagatcttcg tgaagaccct gactggtaag      60

accatcactc tcgaggtgga gccgagtgac accattgaga atgtcaaggc aaagatccaa     120

gacaaggaag gcatccctcc tgaccagcag aggttgatct ttgctgggaa acagctggaa     180

gatggacgca ccctgtctga ctacaacatc cagaaagagt ccaccctgca cctggtgctc     240

cgtctccgcg gtggatcggc ccccagcgcg acccccatct tcgcgcccgg cgagaactgc     300

agccccgcgt ggggggcggc gcccgcgaac tacgacgcag cggacacgca cctgcgcatc     360

atgggcaagc cggtgatgga gcgctgggag accccctata tgcacgcgct ggccgccgcc     420

gcctcctcca aagggggccg ggtcctggag gtgggctttg gcatggccat cgcagcgtca     480

aaggtgcagg aggcgcccat tgatgagcat tggatcatcg agtgcaatga cggcgtcttc     540

cagcggctcc gggactgggc cccacggcag acacacaagg tcatcccctt gaaaggcctg     600

tgggaggatg tggcacccac cctgcctgac ggtcactttg atgggatcct gtacgacacg     660

tacccactct cggaggagac ctggcacaca caccagttca acttcatcaa gaaccacgcc     720

tttcgcctgc tgaagccggg gggcgtcctc acctactgca acctcacctc ctggggggag     780

ctgatgaagt ccaagtactc agacatcacc cagatgtttg aggagacgca ggtgcccgcg     840

ctgctggagg ccggcttccg gagggagaac atccgtacgg aggtgatggc gctgcagcca     900

ccggccgact gccgctacta cgccttccca cagatgatca cgcccctggt gaccaaaggc     960

tga                                                                   963


<210> 8
<211> 963
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 8 – Nucleotidic sequence of His-UB-GAMT mutant CLONE 51
      (L37M-I187Q-V215Q)

<400> 8
atggcacatc accaccacca tcacgtgatg cagatcttcg tgaagaccct gactggtaag      60

accatcactc tcgaggtgga gccgagtgac accattgaga atgtcaaggc aaagatccaa     120

gacaaggaag gcatccctcc tgaccagcag aggttgatct ttgctgggaa acagctggaa     180

gatggacgca ccctgtctga ctacaacatc cagaaagagt ccaccctgca cctggtgctc     240

cgtctccgcg gtggatcggc ccccagcgcg acccccatct tcgcgcccgg cgagaactgc     300

agccccgcgt ggggggcggc gcccgcggcc tacgacgcag cggacacgca cctgcgcatc     360

atgggcaagc cggtgatgga gcgctgggag accccctata tgcacgcgct ggccgccgcc     420

gcctcctcca aagggggccg ggtcctggag gtgggctttg gcatggccat cgcagcgtca     480

aaggtgcagg aggcgcccat tgatgagcat tggatcatcg agtgcaatga cggcgtcttc     540

cagcggctcc gggactgggc cccacggcag acacacaagg tcatcccctt gaaaggcctg     600

tgggaggatg tggcacccac cctgcctgac ggtcactttg atgggatcct gtacgacacg     660

tacccactct cggaggagac ctggcacaca caccagttca acttcatcaa gaaccacgcc     720

tttcgcctgc tgaagccggg gggcgtcctc acctactgca acctcacctc ctggggggag     780

ctgatgaagt ccaagtactc agacatcacc cagatgtttg aggagacgca ggtgcccgcg     840

ctgctggagg ccggcttccg gagggagaac atccgtacgg aggtgatggc gctgcagcca     900

ccggccgact gccgctacta cgccttccca cagatgatca cgcccctggt gaccaaaggc     960

tga                                                                   963


<210> 9
<211> 963
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 9 – Nucleotidic sequence of His-UB-GAMT mutant CLONE 76
      (A26N-L37I-I187Q-V215Q)

<400> 9
atggcacatc accaccacca tcacgtgatg cagatcttcg tgaagaccct gactggtaag      60

accatcactc tcgaggtgga gccgagtgac accattgaga atgtcaaggc aaagatccaa     120

gacaaggaag gcatccctcc tgaccagcag aggttgatct ttgctgggaa acagctggaa     180

gatggacgca ccctgtctga ctacaacatc cagaaagagt ccaccctgca cctggtgctc     240

cgtctccgcg gtggatcggc ccccagcgcg acccccatct tcgcgcccgg cgagaactgc     300

agccccgcgt ggggggcggc gcccgcgaac tacgacgcag cggacacgca cctgcgcatc     360

attggcaagc cggtgatgga gcgctgggag accccctata tgcacgcgct ggccgccgcc     420

gcctcctcca aagggggccg ggtcctggag gtgggctttg gcatggccat cgcagcgtca     480

aaggtgcagg aggcgcccat tgatgagcat tggatcatcg agtgcaatga cggcgtcttc     540

cagcggctcc gggactgggc cccacggcag acacacaagg tcatcccctt gaaaggcctg     600

tgggaggatg tggcacccac cctgcctgac ggtcactttg atgggatcct gtacgacacg     660

tacccactct cggaggagac ctggcacaca caccagttca acttcatcaa gaaccacgcc     720

tttcgcctgc tgaagccggg gggcgtcctc acctactgca acctcacctc ctggggggag     780

ctgatgaagt ccaagtactc agacatcacc cagatgtttg aggagacgca ggtgcccgcg     840

ctgctggagg ccggcttccg gagggagaac atccgtacgg aggtgatggc gctgcagcca     900

ccggccgact gccgctacta cgccttccca cagatgatca cgcccctggt gaccaaaggc     960

tga                                                                   963


<210> 10
<211> 1188
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 10 – Nucleotidic sequence of MAT2A wild-type

<400> 10
atgaacggac agctcaacgg cttccacgag gcgttcatcg aggagggcac attccttttc      60

acctcagagt cggtcgggga aggccaccca gataagattt gtgaccaaat cagtgatgct     120

gtccttgatg cccaccttca gcaggatcct gatgccaaag tagcttgtga aactgttgct     180

aaaactggaa tgatccttct tgctggggaa attacatcca gagctgctgt tgactaccag     240

aaagtggttc gtgaagctgt taaacacatt ggatatgatg attcttccaa aggttttgac     300

tacaagactt gtaacgtgct ggtagccttg gagcaacagt caccagatat tgctcaaggt     360

gttcatcttg acagaaatga agaagacatt ggtgctggag accagggctt aatgtttggc     420

tatgccactg atgaaactga ggagtgtatg cctttaacca ttgtcttggc acacaagcta     480

aatgccaaac tggcagaact acgccgtaat ggcactttgc cttggttacg ccctgattct     540

aaaactcaag ttactgtgca gtatatgcag gatcgaggtg ctgtgcttcc catcagagtc     600

cacacaattg ttatatctgt tcagcatgat gaagaggttt gtcttgatga aatgagggat     660

gccctaaagg agaaagtcat caaagcagtt gtgcctgcga aataccttga tgaggataca     720

atctaccacc tacagccaag tggcagattt gttattggtg ggcctcaggg tgatgctggt     780

ttgactggac ggaaaatcat tgtggacact tatggcggtt ggggtgctca tggaggaggt     840

gccttttcag gaaaggatta taccaaggtc gaccgttcag ctgcttatgc tgctcgttgg     900

gtggcaaaat cccttgttaa aggaggtctg tgccggaggg ttcttgttca ggtctcttat     960

gctattggag tttctcatcc attatctatc tccattttcc attatggtac ctctcagaag    1020

agtgagagag agctattaga gattgtgaag aagaatttcg atctccgccc tggggtcatt    1080

gtcagggatc tggatctgaa gaagccaatt tatcagagga ctgcagccta tggccacttt    1140

ggtagggaca gcttcccatg ggaagtgccc aaaaagctta aatattga                 1188


<210> 11
<211> 1443
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 11 – Nucleotidic sequence of His-UB-MAT2A

<400> 11
atggcacatc accaccacca tcacgtgatg cagatcttcg tgaagaccct gactggtaag      60

accatcactc tcgaggtgga gccgagtgac accattgaga atgtcaaggc aaagatccaa     120

gacaaggaag gcatccctcc tgaccagcag aggttgatct ttgctgggaa acagctggaa     180

gatggacgca ccctgtctga ctacaacatc cagaaagagt ccaccctgca cctggtgctc     240

cgtctccgcg gtggaatgaa cggacagctc aacggcttcc acgaggcgtt catcgaggag     300

ggcacattcc ttttcacctc agagtcggtc ggggaaggcc acccagataa gatttgtgac     360

caaatcagtg atgctgtcct tgatgcccac cttcagcagg atcctgatgc caaagtagct     420

tgtgaaactg ttgctaaaac tggaatgatc cttcttgctg gggaaattac atccagagct     480

gctgttgact accagaaagt ggttcgtgaa gctgttaaac acattggata tgatgattct     540

tccaaaggtt ttgactacaa gacttgtaac gtgctggtag ccttggagca acagtcacca     600

gatattgctc aaggtgttca tcttgacaga aatgaagaag acattggtgc tggagaccag     660

ggcttaatgt ttggctatgc cactgatgaa actgaggagt gtatgccttt aaccattgtc     720

ttggcacaca agctaaatgc caaactggca gaactacgcc gtaatggcac tttgccttgg     780

ttacgccctg attctaaaac tcaagttact gtgcagtata tgcaggatcg aggtgctgtg     840

cttcccatca gagtccacac aattgttata tctgttcagc atgatgaaga ggtttgtctt     900

gatgaaatga gggatgccct aaaggagaaa gtcatcaaag cagttgtgcc tgcgaaatac     960

cttgatgagg atacaatcta ccacctacag ccaagtggca gatttgttat tggtgggcct    1020

cagggtgatg ctggtttgac tggacggaaa atcattgtgg acacttatgg cggttggggt    1080

gctcatggag gaggtgcctt ttcaggaaag gattatacca aggtcgaccg ttcagctgct    1140

tatgctgctc gttgggtggc aaaatccctt gttaaaggag gtctgtgccg gagggttctt    1200

gttcaggtct cttatgctat tggagtttct catccattat ctatctccat tttccattat    1260

ggtacctctc agaagagtga gagagagcta ttagagattg tgaagaagaa tttcgatctc    1320

cgccctgggg tcattgtcag ggatctggat ctgaagaagc caatttatca gaggactgca    1380

gcctatggcc actttggtag ggacagcttc ccatgggaag tgcccaaaaa gcttaaatat    1440

tga                                                                  1443


<210> 12
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 12 – GAMT PmlI_Fwd cloning primer

<400> 12
acggtacttc acgtgatgag cgcccccagc gcga                                 34


<210> 13
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 13 - UB-GAMT NcoI_Fwd cloning primer

<400> 13
acggtacttc catgggcccc cagcgcgacc ccc                                  33


<210> 14
<211> 37
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 14 - GAMT HindIII_Rev cloning primer

<400> 14
ggatacctaa agcttcagcc tttggtcacc aggggcg                              37


<210> 15
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 15 - UB-GAMT (L37I) mutagenesis primer

<400> 15
cacgcacctg cgcatcattg gcaagccggt gatgg                                35


<210> 16
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 16 - UB-GAMT (G38A) mutagenesis primer

<400> 16
acctgcgcat cctggccaag ccggtgatgg agc                                  33


<210> 17
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 17 - UB-GAMT (L37I - G38A) mutagenesis primer

<400> 17
cacgcacctg cgcatcattg ccaagccggt gatggagc                             38


<210> 18
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 18 - UB-GAMT (L37M) mutagenesis primer

<400> 18
acgcacctgc gcatcatggg caagccggtg atgg                                 34


<210> 19
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 19 - UB-GAMT (I187Q) mutagenesis primer

<400> 19
gtactcagac atcacccaga tgtttgagga gacgcaggtg                           40


<210> 20
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 20 - UB-GAMT (A26N) mutagenesis primer

<400> 20
gggcggcgcc cgcgaactac gacgcagcgg ac                                   32


<210> 21
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 21 - UB-GAMT (V215Q) mutagenesis primer

<400> 21
ggaggtgatg gcgctgcagc caccggccga ctgcc                                35


<210> 22
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 22 - GAMT 7*_Fwd sequencing primer

<400> 22
gcccccagcg cgacccccat ct                                             22


<210> 23
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 23 - GAMT 312*_Fwd sequencing primer

<400> 23
acggcagaca cacaaggtca tcc                                            23


<210> 24
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 24 - GAMT 711*_Rev sequencing primer

<400> 24
tcagcctttg gtcaccaggg gc                                             22


<210> 25
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 25 - T7 promoter sequencing primer

<400> 25
taatacgact cactataggg                                                20


<210> 26
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 26 - T7 terminator sequencing primer

<400> 26
gctagttatt gctcagcgg                                                 19


<210> 27
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 27 - MAT2A SacII_Fwd cloning primer

<400> 27
cgtctccgcg gtggaatgaa cggacagctc aacggc                               36


<210> 28
<211> 42
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 28 - MAT2A NotI_Rev cloning primer

<400> 28
cgtctgcggc cgctcaatat ttaagctttt tgggcacttc cc                        42


<210> 29
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 29 - MAT2A 100*_Fwd sequencing primer

<400> 29
gtgaccaaat cagtgatgct gtcc                                           24


<210> 30
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 30 - MAT2A 398*_Fwd sequencing primer

<400> 30
agaccagggc ttaatgtttg gc                                             22


<210> 31
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> SEQ ID NO 31 - MAT2A 799*_Fwd sequencing primer

<400> 31
ttgtggacac ttatggcggt tg                                             22


<210> 32
<211> 236
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQ ID NO 32 – Ammino acid sequence of GAMT wild-type containing
      the starting methionine

<400> 32
Met Ser Ala Pro Ser Ala Thr Pro Ile Phe Ala Pro Gly Glu Asn Cys 
1               5                   10                  15      
Ser Pro Ala Trp Gly Ala Ala Pro Ala Ala Tyr Asp Ala Ala Asp Thr 
            20                  25                  30          
His Leu Arg Ile Leu Gly Lys Pro Val Met Glu Arg Trp Glu Thr Pro 
        35                  40                  45              
Tyr Met His Ala Leu Ala Ala Ala Ala Ser Ser Lys Gly Gly Arg Val 
    50                  55                  60                  
Leu Glu Val Gly Phe Gly Met Ala Ile Ala Ala Ser Lys Val Gln Glu 
65                  70                  75                  80  
Ala Pro Ile Asp Glu His Trp Ile Ile Glu Cys Asn Asp Gly Val Phe 
                85                  90                  95      
Gln Arg Leu Arg Asp Trp Ala Pro Arg Gln Thr His Lys Val Ile Pro 
            100                 105                 110         
Leu Lys Gly Leu Trp Glu Asp Val Ala Pro Thr Leu Pro Asp Gly His 
        115                 120                 125             
Phe Asp Gly Ile Leu Tyr Asp Thr Tyr Pro Leu Ser Glu Glu Thr Trp 
    130                 135                 140                 
His Thr His Gln Phe Asn Phe Ile Lys Asn His Ala Phe Arg Leu Leu 
145                 150                 155                 160 
Lys Pro Gly Gly Val Leu Thr Tyr Cys Asn Leu Thr Ser Trp Gly Glu 
                165                 170                 175     
Leu Met Lys Ser Lys Tyr Ser Asp Ile Thr Ile Met Phe Glu Glu Thr 
            180                 185                 190         
Gln Val Pro Ala Leu Leu Glu Ala Gly Phe Arg Arg Glu Asn Ile Arg 
        195                 200                 205             
Thr Glu Val Met Ala Leu Val Pro Pro Ala Asp Cys Arg Tyr Tyr Ala 
    210                 215                 220                 
Phe Pro Gln Met Ile Thr Pro Leu Val Thr Lys Gly 
225                 230                 235     

