                         SEQUENCE LISTING

<110>  Universidad de Chile
       Fundacin COPEC Universidad Catlica
 
<120>  Method for simultaneous detection and quantification OF Listeria 
       monocytogenes, Salmonella spp., and shiga toxin-producing 
       Escherichia coli (STEC)

<130>  ALBA 173

<160>  96    

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer_bind (forward) to Listeria monocytogenes

<400>  1
aattcttcct tcaaagccgt aa                                                22


<210>  2
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer_bind reverse Listeria monocytogenes

<400>  2
gaggttaccg tcgatgattt g                                                 21


<210>  3
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer_bind forward Listeria monocytogenes

<400>  3
tgcgcaacaa actgaagcaa                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer_bind reverse Listeria monocytogenes

<400>  4
tggcgtctta ggacttgcag                                                   20


<210>  5
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer_bind forward Listeria monocytogenes

<400>  5
tgcatctgca ttcaataaag aaa                                               23


<210>  6
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer_bind reverse Listeria monocytogenes

<400>  6
tccgcgtgtt tcttttcgat tggc                                              24


<210>  7
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer_bind forward Shiga toxin-producing Escherichia coli (STEC)

<400>  7
ctatactccg attcctctgg tga                                               23


<210>  8
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer_bind reverse Shiga toxin-producing Escherichia coli (STEC)

<400>  8
gatcattttc attacccgta cca                                               23


<210>  9
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer_bind forward Shiga toxin-producing Escherichia coli (STEC)

<400>  9
tttaggttcg gcacctcttg                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer_bind reverse Shiga toxin-producing Escherichia coli (STEC)

<400>  10
ccttgtcatc ggtcatgttg                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer_bind forward Shiga toxin-producing Escherichia coli (STEC)

<400>  11
caacatgacc gatgacaagg                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer_bind reverse Shiga toxin-producing Escherichia coli (STEC)

<400>  12
gcggtatctt tcgcgtaatc                                                   20


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer_bind forward Salmonella spp.

<400>  13
cggactcacc aggagattac a                                                 21


<210>  14
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer_bind reverse Salmonella spp.

<400>  14
ggcgagaccg acttttagc                                                    19


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer_bind forward Salmonella spp.

<400>  15
cttttagcga agccatggag                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer_bind reverse Salmonella spp.

<400>  16
gtttacccac gcgtttcatc                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer_bind forward Salmonella spp.

<400>  17
cgctgtttac cggatttctc                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer_bind reverse Salmonella spp.

<400>  18
cggccggtat aaatcaacag                                                   20


<210>  19
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Center region to intenal control (IC)

<400>  19
acgtagtggc ctcggcgcca gtagct                                            26


<210>  20
<211>  70
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example of internal control, contructec with SEQ ID No 13, SEQ ID
       NO 19 and reverse of SEQ ID No 8 (i.e. SEQ ID No 39)

<400>  20
cggactcacc aggagattac aacgtagtgg cctcggcgcc agtagcttgg tacgggtaat       60

gaaaatgatc                                                              70


<210>  21
<211>  68
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 1, SEQ ID 
       No 9, and reverse complementary of SEQ ID No 10 (i.e. SEQ ID No 40)

<400>  21
aattcttcct tcaaagccgt aaacgtagtg gcctcggcgc cagtagctca acatgaccga       60

tgacaagg                                                                68


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe to Listeria monocytogenes

<400>  22
ccgccaagaa aaggttacaa                                                   20


<210>  23
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe to Listeria monocytogenes

<400>  23
cggaggttcc gcaaaagatg a                                                 21


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe to Listeria monocytogenes

<400>  24
atctgcattc aataaagaaa a                                                 21


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe to Listeria monocytogenes

<400>  25
catccatggc accaccagca tct                                               23


<210>  26
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe to Shiga toxin-producing Escherichia coli (STEC)

<400>  26
aggtggtgtt gctggtcaca cgaat                                             25


<210>  27
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe to Shiga toxin-producing Escherichia coli (STEC)

<400>  27
cgatggggat cgattaccgt ca                                                22


<210>  28
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe to Shiga toxin-producing Escherichia coli (STEC)

<400>  28
taaattatgc ggcacaacag gcgg                                              24


<210>  29
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe to Shiga toxin-producing Escherichia coli (STEC)

<400>  29
tgctggtcac acgaataaac tga                                               23


<210>  30
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe to Salmonella spp.

<400>  30
catggctaat ttaacccgtc gtcagtg                                           27


<210>  31
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe to Salmonella spp.

<400>  31
gtggttcctg gcgcagcaaa taaaac                                            26


<210>  32
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe to Salmonella spp.

<400>  32
gagaaagcgg tcttgacgcc cgctcaa                                           27


<210>  33
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe to Salmonella spp.

<400>  33
ctggcgcagc aaataaaacg attattc                                           27


<210>  34
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe to Internal control

<400>  34
cagtggcctc ggcgccagta g                                                 21


<210>  35
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe to Internal control

<400>  35
ccgtagtggc ctcggcgcca g                                                 21


<210>  36
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement  of primer_bind reverse 2 Listeria 
       monocytogenes

<400>  36
caaatcatcg acggtaacct c                                                 21


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement  of primer_bind reverse 4 Listeria 
       monocytogenes

<400>  37
ctgcaagtcc taagacgcca                                                   20


<210>  38
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement of primer_bind reverse 6 Listeria 
       monocytogenes

<400>  38
gccaatcgaa aagaaacacg cgga                                              24


<210>  39
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement primer_bind reverse 8 Shiga toxin-producing 
       Escherichia coli (STEC)

<400>  39
tggtacgggt aatgaaaatg atc                                               23


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement primer_bind reverse 10 Shiga toxin-producing 
       Escherichia coli (STEC)

<400>  40
caacatgacc gatgacaagg                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement primer_bind reverse 12 Shiga toxin-producing 
       Escherichia coli (STEC)

<400>  41
gattacgcga aagataccgc                                                   20


<210>  42
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement primer_bind reverse 14 Salmonella spp.

<400>  42
gctaaaagtc ggtctcgcc                                                    19


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement primer_bind reverse 16 Salmonella spp.

<400>  43
gatgaaacgc gtgggtaaac                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse complement primer_bind reverse 18 Salmonella spp.

<400>  44
ctgttgattt ataccggccg                                                   20


<210>  45
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 1, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 8 (i.e. SEQ ID No 39)

<400>  45
aattcttcct tcaaagccgt aaacgtagtg gcctcggcgc cagtagcttg gtacgggtaa       60

tgaaaatgat c                                                            71


<210>  46
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 1, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 12  (i.e.SEQ ID No 
       41)

<400>  46
aattcttcct tcaaagccgt aaacgtagtg gcctcggcgc cagtagctga ttacgcgaaa       60

gataccgc                                                                68


<210>  47
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 1, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 14 (i.e. SEQ ID No 
       42)

<400>  47
aattcttcct tcaaagccgt aaacgtagtg gcctcggcgc cagtagctgc taaaagtcgg       60

tctcgcc                                                                 67


<210>  48
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 1, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 16 (i.e. SEQ ID No 
       43)

<400>  48
aattcttcct tcaaagccgt aaacgtagtg gcctcggcgc cagtagctga tgaaacgcgt       60

gggtaaac                                                                68


<210>  49
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 1, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 18 (i.e. SEQ ID No 
       44)

<400>  49
aattcttcct tcaaagccgt aaacgtagtg gcctcggcgc cagtagctct gttgatttat       60

accggccg                                                                68


<210>  50
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 3, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 8 (i.e. SEQ ID No 
       39)

<400>  50
tgcgcaacaa actgaagcaa acgtagtggc ctcggcgcca gtagcttggt acgggtaatg       60

aaaatgatc                                                               69


<210>  51
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 3, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 10 (i.e. SEQ ID No 
       40)

<400>  51
tgcgcaacaa actgaagcaa acgtagtggc ctcggcgcca gtagctcaac atgaccgatg       60

acaagg                                                                  66


<210>  52
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 3, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 12 (i.e. SEQ ID No 
       41)

<400>  52
tgcgcaacaa actgaagcaa acgtagtggc ctcggcgcca gtagctgatt acgcgaaaga       60

taccgc                                                                  66


<210>  53
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 3, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 14 (i.e. SEQ ID No 
       42)

<400>  53
tgcgcaacaa actgaagcaa acgtagtggc ctcggcgcca gtagctgcta aaagtcggtc       60

tcgcc                                                                   65


<210>  54
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 3, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 16 (i.e. SEQ ID No 
       43)

<400>  54
tgcgcaacaa actgaagcaa acgtagtggc ctcggcgcca gtagctgatg aaacgcgtgg       60

gtaaac                                                                  66


<210>  55
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 3, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 18 (i.e. SEQ ID No 
       44)

<400>  55
tgcgcaacaa actgaagcaa acgtagtggc ctcggcgcca gtagctctgt tgatttatac       60

cggccg                                                                  66


<210>  56
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 5, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 8 (i.e. SEQ ID No 
       39)

<400>  56
tgcatctgca ttcaataaag aaaacgtagt ggcctcggcg ccagtagctt ggtacgggta       60

atgaaaatga tc                                                           72


<210>  57
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 5, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 10  (i.e. SEQ ID No
       40)

<400>  57
tgcatctgca ttcaataaag aaaacgtagt ggcctcggcg ccagtagctc aacatgaccg       60

atgacaagg                                                               69


<210>  58
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 5, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 12 (i.e. SEQ ID No 
       41)

<400>  58
tgcatctgca ttcaataaag aaaacgtagt ggcctcggcg ccagtagctg attacgcgaa       60

agataccgc                                                               69


<210>  59
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 5, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 14 (i.e. SEQ ID No 
       42)

<400>  59
tgcatctgca ttcaataaag aaaacgtagt ggcctcggcg ccagtagctg ctaaaagtcg       60

gtctcgcc                                                                68


<210>  60
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 5, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 16 (i.e. SEQ ID No 
       43)

<400>  60
tgcatctgca ttcaataaag aaaacgtagt ggcctcggcg ccagtagctg atgaaacgcg       60

tgggtaaac                                                               69


<210>  61
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 5, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 18 (i.e. SEQ ID No 
       44)

<400>  61
tgcatctgca ttcaataaag aaaacgtagt ggcctcggcg ccagtagctc tgttgattta       60

taccggccg                                                               69


<210>  62
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 7, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 2 (i.e. SEQ ID No 
       36)

<400>  62
ctatactccg attcctctgg tgaacgtagt ggcctcggcg ccagtagctc aaatcatcga       60

cggtaacctc                                                              70


<210>  63
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 7, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 4  (i.e. SEQ ID No 
       37)

<400>  63
ctatactccg attcctctgg tgaacgtagt ggcctcggcg ccagtagctc tgcaagtcct       60

aagacgcca                                                               69


<210>  64
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 7, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 6 (i.e. SEQ ID No 
       38)

<400>  64
ctatactccg attcctctgg tgaacgtagt ggcctcggcg ccagtagctg ccaatcgaaa       60

agaaacacgc gga                                                          73


<210>  65
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 7, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 14 (i.e. SEQ ID No 
       42)

<400>  65
ctatactccg attcctctgg tgaacgtagt ggcctcggcg ccagtagctg ctaaaagtcg       60

gtctcgcc                                                                68


<210>  66
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 7, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 16 (i.e. SEQ ID No 
       43)

<400>  66
ctatactccg attcctctgg tgaacgtagt ggcctcggcg ccagtagctg atgaaacgcg       60

tgggtaaac                                                               69


<210>  67
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 7, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 18 (i.e. SEQ ID No 
       44)

<400>  67
ctatactccg attcctctgg tgaacgtagt ggcctcggcg ccagtagctc tgttgattta       60

taccggccg                                                               69


<210>  68
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No9, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 2  (i.e. SEQ ID No 
       36)

<400>  68
tttaggttcg gcacctcttg acgtagtggc ctcggcgcca gtagctcaaa tcatcgacgg       60

taacctc                                                                 67


<210>  69
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 9, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 4 (i.e. SEQ ID No 
       37)

<400>  69
tttaggttcg gcacctcttg acgtagtggc ctcggcgcca gtagctctgc aagtcctaag       60

acgcca                                                                  66


<210>  70
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 9, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 6 (i.e. SEQ ID No 
       38)

<400>  70
tttaggttcg gcacctcttg acgtagtggc ctcggcgcca gtagctgcca atcgaaaaga       60

aacacgcgga                                                              70


<210>  71
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 9, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 14 (i.e. SEQ ID No 
       42)

<400>  71
tttaggttcg gcacctcttg acgtagtggc ctcggcgcca gtagctgcta aaagtcggtc       60

tcgcc                                                                   65


<210>  72
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 9, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 16 (i.e. SEQ ID No 
       43)

<400>  72
tttaggttcg gcacctcttg acgtagtggc ctcggcgcca gtagctgatg aaacgcgtgg       60

gtaaac                                                                  66


<210>  73
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 9, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 18 (i.e. SEQ ID No 
       44)

<400>  73
tttaggttcg gcacctcttg acgtagtggc ctcggcgcca gtagctctgt tgatttatac       60

cggccg                                                                  66


<210>  74
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No11, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 2  (i.e. SEQ ID No 
       36)

<400>  74
caacatgacc gatgacaagg acgtagtggc ctcggcgcca gtagctcaaa tcatcgacgg       60

taacctc                                                                 67


<210>  75
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 11, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 4 (i.e. SEQ ID No 
       37)

<400>  75
caacatgacc gatgacaagg acgtagtggc ctcggcgcca gtagctctgc aagtcctaag       60

acgcca                                                                  66


<210>  76
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 11, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 6 (i.e. SEQ ID No 
       38)

<400>  76
caacatgacc gatgacaagg acgtagtggc ctcggcgcca gtagctgcca atcgaaaaga       60

aacacgcgga                                                              70


<210>  77
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 11, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 14 (i.e. SEQ ID No 
       42)

<400>  77
caacatgacc gatgacaagg acgtagtggc ctcggcgcca gtagctgcta aaagtcggtc       60

tcgcc                                                                   65


<210>  78
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 11, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 16 (i.e. SEQ ID No 
       43)

<400>  78
caacatgacc gatgacaagg acgtagtggc ctcggcgcca gtagctgatg aaacgcgtgg       60

gtaaac                                                                  66


<210>  79
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 11, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 18 (i.e. SEQ ID No 
       44)

<400>  79
caacatgacc gatgacaagg acgtagtggc ctcggcgcca gtagctctgt tgatttatac       60

cggccg                                                                  66


<210>  80
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No13, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 2  (i.e. SEQ ID No 
       36)

<400>  80
cggactcacc aggagattac aacgtagtgg cctcggcgcc agtagctcaa atcatcgacg       60

gtaacctc                                                                68


<210>  81
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 13, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 4 (i.e. SEQ ID No 
       37)

<400>  81
cggactcacc aggagattac aacgtagtgg cctcggcgcc agtagctctg caagtcctaa       60

gacgcca                                                                 67


<210>  82
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 13, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 6 (i.e. SEQ ID No 
       38)

<400>  82
cggactcacc aggagattac aacgtagtgg cctcggcgcc agtagctgcc aatcgaaaag       60

aaacacgcgg a                                                            71


<210>  83
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 13, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 10  (i.e. SEQ ID No
       40)

<400>  83
cggactcacc aggagattac aacgtagtgg cctcggcgcc agtagctcaa catgaccgat       60

gacaagg                                                                 67


<210>  84
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 13, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 12 (i.e. SEQ ID No 
       41)

<400>  84
cggactcacc aggagattac aacgtagtgg cctcggcgcc agtagctgat tacgcgaaag       60

ataccgc                                                                 67


<210>  85
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No15, SEQ ID  
       No 19, and reverse complementary of SEQ ID No 2 (i.e. SEQ ID No 
       36)

<400>  85
cttttagcga agccatggag acgtagtggc ctcggcgcca gtagctcaaa tcatcgacgg       60

taacctc                                                                 67


<210>  86
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 15, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 4 (i.e. SEQ ID No 
       37)

<400>  86
cttttagcga agccatggag acgtagtggc ctcggcgcca gtagctctgc aagtcctaag       60

acgcca                                                                  66


<210>  87
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 15, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 6 (i.e. SEQ ID No 
       38)

<400>  87
cttttagcga agccatggag acgtagtggc ctcggcgcca gtagctgcca atcgaaaaga       60

aacacgcgga                                                              70


<210>  88
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 15, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 8 (i.e. SEQ ID No 
       39)

<400>  88
cttttagcga agccatggag acgtagtggc ctcggcgcca gtagcttggt acgggtaatg       60

aaaatgatc                                                               69


<210>  89
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 15, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 10  (i.e. SEQ ID No
       40)

<400>  89
cttttagcga agccatggag acgtagtggc ctcggcgcca gtagctcaac atgaccgatg       60

acaagg                                                                  66


<210>  90
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 15, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 12 (i.e. SEQ ID No 
       41)

<400>  90
cttttagcga agccatggag acgtagtggc ctcggcgcca gtagctgatt acgcgaaaga       60

taccgc                                                                  66


<210>  91
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 17, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 2  (i.e. SEQ ID No 
       36)

<400>  91
cgctgtttac cggatttctc acgtagtggc ctcggcgcca gtagctcaaa tcatcgacgg       60

taacctc                                                                 67


<210>  92
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 17, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 4 (i.e. SEQ ID No 
       37)

<400>  92
cgctgtttac cggatttctc acgtagtggc ctcggcgcca gtagctctgc aagtcctaag       60

acgcca                                                                  66


<210>  93
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 17, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 6 o(i.e. SEQ ID No 
       38)

<400>  93
cgctgtttac cggatttctc acgtagtggc ctcggcgcca gtagctgcca atcgaaaaga       60

aacacgcgga                                                              70


<210>  94
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 17, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 8 (i.e. SEQ ID No 
       39)

<400>  94
cgctgtttac cggatttctc acgtagtggc ctcggcgcca gtagcttggt acgggtaatg       60

aaaatgatc                                                               69


<210>  95
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 17, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 10  (i.e. SEQ ID No
       40)

<400>  95
cgctgtttac cggatttctc acgtagtggc ctcggcgcca gtagctcaac atgaccgatg       60

acaagg                                                                  66


<210>  96
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of Intenal control constructed with SEQ ID No 17, SEQ ID 
       No 19, and reverse complementary of SEQ ID No 12  (i.e. SEQ ID No
       41)

<400>  96
cgctgtttac cggatttctc acgtagtggc ctcggcgcca gtagctgatt acgcgaaaga       60

taccgc                                                                  66


