               SEQUENCE LISTING

<110> ASOCIACIÓN CENTRO DE INVESTIGACIÓN COOPERATIVA EN NANOCIENCIAS “CIC nanoGUNE"

<120> SYNTHETIC CAS PROTEINS

<130> IBIO-2300/PCT

<150> EP22165690.3
<151> 2022-03-30
<150> GB2107671.6
<151> 2021-05-28
<150> EP21382474.1
<151> 2021-05-25

<160> 246

<170> BiSSAP 1.3.6

<210> 1
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> Last Firmicute Common Ancestor (LFCA Cas9) - Node 63

<400> 1
Met Lys Lys Asp Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Asp Asn Tyr Asn Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Ser Ile Gly Lys Ala Leu Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Ala Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
His Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Gln Arg Ile Asp 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Gln Glu Glu Ile Ser Lys Val Asp Pro Ser 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Asp Asp Arg His Pro Leu Phe Gly Asp Asn Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Met Glu 
    130                 135                 140                 
Ser Asp Glu Lys His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Leu Val Gln Val Phe 
            180                 185                 190         
Asn Asp Thr Phe Glu Glu Glu His Leu Ser Glu Glu Ala Ile Asp Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Arg Ser Thr Arg Ala Lys Glu 
    210                 215                 220                 
Val Val Lys Leu Phe Gly Ser Glu Lys Lys Gln Ala Cys Ile Ser Ala 
225                 230                 235                 240 
Leu Ile Lys Leu Ile Val Gly Leu Lys Gly Asn Leu Lys Lys Val Phe 
                245                 250                 255     
Gly Asp Ala Glu Asp Thr Ser Ile His Phe Ser Lys Asp Asn Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Ile Arg Asp Ile Ile Pro Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Glu Ala Ile Lys Ala Leu Tyr Asp Ala Ile Val Leu Ser Gly 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Ser Asn Thr Lys Ala Lys Val Ser Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Gln His Gln Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Asn Thr Lys Asn Gly Tyr Ser Ala Tyr Ile Glu Gly Gly Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Asn Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Thr Ser Asp Asn Gly Val Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Ser Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Gly Arg Phe Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Lys Asp Gly Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Asn Cys Thr Tyr Leu Pro Gly Glu Asn Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Leu Asn Glu Leu Asn Asn Val Arg 
        515                 520                 525             
Leu Thr Thr Asp Lys Gly Lys Thr Arg Arg Phe Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Arg Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Glu Asp Tyr Leu Lys Arg Glu Tyr Glu Glu Phe Asp Ser 
                565                 570                 575     
Ala Asp Ile Ser Gly Ile Asp Gly Glu Phe Lys Ser Ser Leu Gly Ser 
            580                 585                 590         
Tyr His Asp Phe Cys Lys Ile Val Val Lys Gly Asn Ser Leu Asp Glu 
        595                 600                 605             
Glu Asp Tyr Lys Asp Ile Ile Glu Glu Ile Ile Lys Trp Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Leu Arg Arg Arg Leu Glu Lys Tyr Ser Glu 
625                 630                 635                 640 
Ile Leu Thr Glu Glu Gln Ile Lys Lys Ile Cys Lys Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Ile Arg Asp Lys 
            660                 665                 670         
Glu Thr Gly Lys Ser Ile Ile Asp Val Leu Arg Glu Thr Asp Ser Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Leu Ser Asp Glu Asp Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Gln Ala Asn Ala Glu Ala Glu Gly Glu Asn Leu His 
705                 710                 715                 720 
Glu Ile Val Glu Asp Leu Pro Gly Ser Pro Ala Ile Lys Arg Gly Ile 
                725                 730                 735     
Leu Gln Ala Leu Lys Ile Val Asp Glu Ile Val Lys Val Met Gly His 
            740                 745                 750         
Glu Pro Lys Asn Ile Phe Val Glu Met Ala Arg Glu Asn Gln Lys Thr 
        755                 760                 765             
Gly Arg Gly Arg Arg Ser Thr Lys Lys Arg Leu Lys Arg Leu Gln Glu 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asn Leu Leu Lys Glu Leu Pro Arg Asp 
785                 790                 795                 800 
Asp Asn Glu Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Thr Gln Met 
                805                 810                 815     
Gly Lys Cys Met Tyr Thr Gly Glu Pro Ile Asp Leu Asp Asp Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Val Ser Arg Glu Glu Asn Ala Arg Lys 
    850                 855                 860                 
Thr Asp Asn Phe Pro Ser Pro Glu Ile Arg Arg Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Met Leu Leu Lys Ala Gly Leu Ile Ser Lys Lys Lys Phe Asp 
                885                 890                 895     
Arg Leu Thr Arg Ala Asp Arg Gly Asp Phe Thr Asp Asp Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Thr Leu Leu Lys Gln Arg Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Arg Asn Ala Lys Ile Val Ser Val Lys Ala Asn Leu 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Gly Leu Tyr Lys Cys Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Asn Ala Leu Leu Lys Lys Tyr Pro Gln Leu Ala Ala Glu Phe Ile Lys 
        995                 1000                1005            
Gly Asp Tyr Arg Lys Asn Asn Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Met His Phe Tyr Ser Asn Ile Met Asn Ser Phe Thr Ser Asp Val 
1025                1030                1035                1040
Lys Ile Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Ile Ala Thr Val Arg Lys Val Met Asn Tyr His Gln Val Leu Ile Thr 
            1060                1065                1070        
Arg Lys Val Glu Glu Glu Lys Gly Gly Phe Phe Asp Gln Thr Ile Leu 
        1075                1080                1085            
Ser Lys Gly Asn Ser Lys Lys Leu Ile Pro Leu Lys Lys Asn Leu Asp 
    1090                1095                1100                
Pro Glu Lys Tyr Gly Gly Tyr Asn Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Glu Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Gln Leu Val Gly Ile Pro Ile Arg Glu Arg Ala Lys Leu Glu 
            1140                1145                1150        
Lys Asn Pro Ile Lys Tyr Leu Glu Lys Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Val Asp Leu Leu Ile Lys Ile Pro Lys Asn Ser Leu Phe Glu Leu Asp 
    1170                1175                1180                
Gly Gly Arg Arg Arg Ile Leu Ala Ala Ala Lys Glu Leu Lys Asn Ala 
1185                1190                1195                1200
Asn Gln Leu Val Leu Pro Ala Glu Glu Tyr Thr Leu Leu Asp Lys Val 
                1205                1210                1215    
Ala Lys Ile Ile Lys Lys Asn Asn Ser Glu Ser Ile Glu Tyr Val Glu 
            1220                1225                1230        
Glu His Leu Ser Glu Phe Asp Glu Leu Leu Glu Ser Leu Ile Asp Tyr 
        1235                1240                1245            
Ser Pro Lys Leu Ala Leu Gln Asp Lys Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Ala Phe Glu Gln Leu Asn Leu Ala Asp Lys Lys Glu Val Ala Lys Glu 
1265                1270                1275                1280
Ile Ile Asn Leu Leu His Cys Thr Ala Thr Thr Ala Asn Ala Ala Leu 
                1285                1290                1295    
Lys Phe Leu Gly Gly Ser Lys Asn Arg Met Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Ser Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 2
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> Last Bacilli Common Ancestor (LBCA Cas9) - node 71

<400> 2
Met Lys Lys Asp Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Ser Ile Lys Lys Asn Leu Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Ser Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Asn Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Phe Val Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Glu Glu Gln His Leu Ser Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Lys Ser Arg Arg Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Leu Ile Lys Leu Ile Val Gly Leu Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Ala Met Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Asp Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Arg Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Val Thr Asp Arg Gly Lys Thr Gln Asn Phe Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Glu Asn Tyr Leu Lys Lys Glu Tyr Glu Tyr Phe Asp Ser 
                565                 570                 575     
Pro Asp Ile Thr Gly Ile Glu Asp Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Ser Lys Asp Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Leu Thr Glu Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ser Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Val Ile Gly Glu Thr Glu Asn Leu His 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Arg Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asn Leu Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Glu Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu Asp Ser Arg Phe Asn Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Arg Lys Asn Asn Ala Arg Glu Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys His Phe Tyr Ser Asn Ile Met Asn Phe Phe Ala Ser Glu Val 
1025                1030                1035                1040
Lys Ile Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Ile Ala Thr Val Arg Lys Val Leu Ser Tyr His Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Thr Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Tyr Leu Glu Lys Lys Gly Tyr Gln Asn Pro Gln 
        1155                1160                1165            
Glu Asp Val Leu Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Asp Lys Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Glu 
            1220                1225                1230        
Glu His Arg Asn Glu Phe Asp Glu Leu Leu Asp Tyr Ile Ile Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Gln Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Met Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Thr Ile Asp Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 3
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> Last Streotococci Common Ancestor (LSCA Cas9) - node 91

<400> 3
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Gln Phe Val Glu Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Glu Glu Ser His Leu Ser Glu Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Ala Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Asp Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Leu His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Arg Gly Lys Thr Gln Phe Phe Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Lys Asp Tyr Leu Glu Lys Glu Phe Glu Glu Phe Asp Ser 
                565                 570                 575     
Val Asp Ile Thr Gly Val Glu Asp Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Leu Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu His 
705                 710                 715                 720 
Glu Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Arg Arg Asn Ser Arg Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Glu Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys Lys Tyr Asn Asp Arg Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Lys Lys Gly Tyr Gln Asn Ile Gln 
        1155                1160                1165            
Glu Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Asp Lys Ser Asn Asn Ser Glu Asn Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Lys Asn Glu Phe Asp Glu Leu Leu Asp Tyr Ile Ile Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Leu Tyr Asp Gln Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Val Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 4
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> Last Pyogenic Common Ancestor (LPCA Cas9) - node 92

<400> 4
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe His Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asp Gln Leu Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Gln Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Asn Asp Glu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Phe Leu Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     
Val Asp Ile Thr Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala Asp 
625                 630                 635                 640 
Leu Phe Asp Lys Lys Val Leu Lys Lys Leu Lys Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Val Ile Gly Gln Thr Asp Ser Leu His 
705                 710                 715                 720 
Glu Val Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Thr Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ala Gln Gly Ile Lys Asn Ser Arg Gln Arg Met Lys Arg Leu Glu Glu 
    770                 775                 780                 
Val Leu Lys Lys Leu Gly Ser Asn Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Thr Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Thr Ser Ser Glu Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys Ser Tyr 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ser Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Pro Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Lys Leu 
945                 950                 955                 960 
Val Ser Asp Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys Lys Tyr Asp Asp Lys Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asn Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Lys Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Ala Lys Gly Tyr Gln Asp Ile Gln 
        1155                1160                1165            
Glu Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Phe Leu Tyr His Ala 
                1205                1210                1215    
Ser Arg Ile Asp Lys Ser Thr Ser Ser Glu Asn Leu Glu Tyr Val Glu 
            1220                1225                1230        
Gln His Lys His Glu Phe Asp Glu Ile Leu Asp Tyr Ile Ile Asp Phe 
        1235                1240                1245            
Ser Glu Arg Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ser 
    1250                1255                1260                
Leu Tyr Asn Gln Asn Asp Asp Ser Asp Ile Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Phe Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Ala Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Val Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp 
    1330                1335                1340

<210> 5
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> Last Pyogenic Common Ancestor (LPCA Cas9) - node 95

<400> 5
Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Phe Leu Ser Gly Glu Gln Lys 
    530                 535                 540                 
Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     
Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala His 
625                 630                 635                 640 
Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu His 
705                 710                 715                 720 
Glu Gln Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu Glu 
    770                 775                 780                 
Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val Glu 
785                 790                 795                 800 
Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu Ser 
            820                 825                 830         
Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn Tyr 
865                 870                 875                 880 
Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu Asn 
    930                 935                 940                 
Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys Leu 
945                 950                 955                 960 
Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys Val Tyr Asp Glu Gln Glu Ile Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Arg Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile 
1025                1030                1035                1040
Thr Leu Ala Asn Glu Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Met Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Arg Asn Ser Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Lys Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Ser 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Asp Phe Leu Glu Ala Lys Gly Tyr Lys Asp Val Gln 
        1155                1160                1165            
Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala Lys Leu Val Thr Phe Leu Tyr His Ala 
                1205                1210                1215    
Ser His Ile Glu Lys Ser Lys Ser Pro Glu Asn Asn Ala Tyr Val Glu 
            1220                1225                1230        
Gln His Lys His Asp Leu Asp Glu Ile Leu Glu Tyr Ile Ser Glu Phe 
        1235                1240                1245            
Ser Lys Arg Tyr Ile Leu Ala Asp Lys Asn Leu Ser Lys Val Lys Ser 
    1250                1255                1260                
Leu Phe Asn Lys His Glu Asp Ser Ser Ile Ser Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Ile Ile Asn Leu Phe Thr Leu Thr Ser Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Leu Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp 
    1330                1335                1340

<210> 6
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> PAM library construction forward primer

<400> 6
aataggcgta tcacgaggc                                                 19


<210> 7
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> PAM library construction reverse primer

<400> 7
agcgagtcag tgagcgag                                                  18


<210> 8
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> HEK293T cell genome editing foward primer

<400> 8
tattgttcct ccgtgcgtca g                                              21


<210> 9
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> HEK293T cell genome editing reverse primer

<400> 9
gacgagaaac acagcccca                                                 19


<210> 10
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #60

<400> 10
Met Lys Lys Asp Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Asp Asn Tyr Asn Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Ser Ile Gly Lys Ala Leu Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Ala Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
His Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Trp Arg Ile Asp 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Gln Glu Glu Ile Ser Lys Val Asp Ser Ser 
                85                  90                  95      
Phe Phe Ile Arg Leu Lys Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Asp Asp Arg His Pro Leu Phe Gly Asp Asn Glu Thr Glu Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Met Glu 
    130                 135                 140                 
Ser Asp Glu Lys His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Ser Ser 
                165                 170                 175     
Glu Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Leu Val Gln Val Leu 
            180                 185                 190         
Asn Asp Thr Leu Glu Glu Glu His Leu Ser Glu Glu Ala Asn Asp Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Arg Ser Thr Arg Ala Lys Glu 
    210                 215                 220                 
Val Val Lys Leu Phe Gly Ser Glu Lys Lys Glu Ala Cys Ile Ser Ala 
225                 230                 235                 240 
Leu Val Lys Leu Ile Val Gly Leu Lys Gly Asn Leu Lys Lys Val Phe 
                245                 250                 255     
Gly Asp Ala Glu Asp Thr Ala Ile His Phe Ser Lys Asp Asn Tyr Glu 
            260                 265                 270         
Glu Asp Val Glu Ala Ile Arg Asp Leu Ile Pro Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Glu Ala Ile Lys Ala Leu Tyr Asp Ala Ile Val Leu Ser Gly 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Ser Asn Thr Lys Ala Lys Val Ser Ala Ser 
305                 310                 315                 320 
Met Val Ala Arg Tyr Glu Gln His Gln Lys Asp Leu Lys Leu Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu His Leu Ser Glu Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Asn Thr Asn Asn Gly Tyr Ser Ala Tyr Ile Glu Gly Gly Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Leu Leu Lys Glu Leu Glu 
    370                 375                 380                 
Asp Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Asn Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Thr Ser Asp Asn Gly Val Ile Pro Tyr Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Ala Glu Lys Ile Glu Ser Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Gly Arg Phe Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Lys Asp Gly Val Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Gly Asn Cys Thr Tyr Leu Pro Gly Glu Thr Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Leu Asn Glu Leu Asn Asn Val Arg 
        515                 520                 525             
Leu Thr Ile Asp Lys Gly Lys Arg Arg Arg Phe Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Arg Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Val Glu Asp Tyr Leu Lys Arg Glu Tyr Glu Asn Phe Asp Ser 
                565                 570                 575     
Ala Asp Ile Ser Gly Ile Asp Gly Glu Phe Lys Ser Ser Leu Gly Ser 
            580                 585                 590         
Tyr His Asp Phe Cys Lys Ile Val Val Lys Ala Asn Ser Leu Asp Glu 
        595                 600                 605             
Glu Asp Tyr Lys Asp Ile Ile Glu Glu Ile Ile Lys Trp Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Leu Arg Arg Arg Leu Glu Lys Tyr Gly Glu 
625                 630                 635                 640 
Ile Leu Thr Glu Glu Gln Ile Lys Lys Ile Cys Lys Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Ile Arg Asp Lys 
            660                 665                 670         
Glu Thr Gly Lys Ser Ile Ile Asp Val Leu Arg Glu Thr Asp Pro Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Leu Ser Asp Glu Asp Leu Gly Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Gln Ala Asn Ala Glu Ala Ala Gly Glu Asn Leu Gln 
705                 710                 715                 720 
Glu Ile Val Asp Asp Leu Pro Gly Ser Pro Ala Ile Lys Arg Gly Ile 
                725                 730                 735     
Asn Gln Ala Leu Arg Ile Val Asp Glu Ile Val Lys Val Met Gly Lys 
            740                 745                 750         
Glu Pro Ser Asn Ile Phe Val Glu Met Ala Arg Glu Asn Gln Gln Ser 
        755                 760                 765             
Ala Arg Gly Arg Arg Ser Thr Lys Lys Arg Leu Lys Arg Leu Gln Glu 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asp Leu Leu Lys Glu Leu Leu Arg Asp 
785                 790                 795                 800 
Asp Asn Glu Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Thr Gln Met 
                805                 810                 815     
Gly Lys Cys Met Tyr Thr Gly Glu Pro Ile Asp Leu Asp Asp Leu Ser 
            820                 825                 830         
Lys Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Val Ser Arg Glu Glu Asn Ala Arg Lys 
    850                 855                 860                 
Thr Asp Asn Leu Pro Ser Pro Glu Ile Arg Arg Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Met Leu Leu Lys Ala Gly Leu Ile Ser Lys Lys Lys Phe Asp 
                885                 890                 895     
Arg Leu Thr Arg Ala Asp Arg Gly Asp Phe Thr Asp Asp Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Thr Leu Leu Lys Gln Arg Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Arg Asn Ala Lys Ile Val Ser Val Lys Ala Asn Leu 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Gly Leu Tyr Lys Cys Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Asn Ala Leu Leu Lys Arg Tyr Pro Ser Leu Ala Ala Glu Phe Ile Lys 
        995                 1000                1005            
Gly Asp Tyr Arg Lys Asn Asn Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Met His Phe Tyr Ser Ser Ile Met Asn Ser Phe Thr Ser Asp Glu 
1025                1030                1035                1040
Lys Ile Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Ile Ala Thr Val Arg Lys Val Met Asn Tyr Arg Gln Val Leu Ile Thr 
            1060                1065                1070        
Arg Lys Val Glu Glu Glu Thr Gly Gly Phe Phe Asp Gln Thr Ile Tyr 
        1075                1080                1085            
Ser Lys Gly Asn Ser Lys Lys Leu Ile Pro Leu Lys Lys Asn Leu Asp 
    1090                1095                1100                
Pro Asp Lys Tyr Gly Gly Tyr Ser Ser Pro Lys Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Glu Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Gln Leu Val Gly Ile Pro Ile Arg Glu Arg Ala Lys Leu Glu 
            1140                1145                1150        
Lys Asn Pro Ser Lys Tyr Leu Glu Lys Lys Gly Tyr Glu Asn Pro Lys 
        1155                1160                1165            
Val Asp Leu Leu Ile Lys Ile Pro Lys Asn Gln Leu Leu Glu Leu Asp 
    1170                1175                1180                
Gly Gly Arg Arg Arg Ile Leu Ala Ala Ala Lys Glu Leu Lys Asn Ala 
1185                1190                1195                1200
Asn Gln Leu Val Leu Pro Ala Glu Glu Tyr Thr Leu Leu Asp Lys Val 
                1205                1210                1215    
Ala Lys Ile Ala Lys Lys Asn Asn Ser Glu Ser Ile Glu Tyr Val Glu 
            1220                1225                1230        
Glu His Leu Ser Glu Phe Asp Glu Leu Leu Glu Ser Leu Ile Asp Tyr 
        1235                1240                1245            
Ser Pro Lys Leu Ala Leu Gln Asp Asn Asn Leu Glu Lys Leu Lys Glu 
    1250                1255                1260                
Ala Phe Glu Gln Leu Asn Leu Ala Asp Lys Lys Asp Val Ala Lys Glu 
1265                1270                1275                1280
Ile Ile Gly Leu Leu His Cys Thr Ala Thr Thr Ala Asn Ala Ala Leu 
                1285                1290                1295    
Lys Ser Leu Gly Gly Ser Lys Asn Arg Met Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Ser Leu Leu Asn Ala Ser Leu Ile His Gln Ser Ile Thr Gly Leu Phe 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 11
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #61

<400> 11
Met Lys Lys Asp Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Asp Asn Tyr Asn Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Ser Ile Gly Lys Ala Leu Leu 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Ala Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
His Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Trp Arg Leu Asp 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Gln Glu Glu Ile Ser Lys Val Asp Ser Ser 
                85                  90                  95      
Phe Phe Ile Arg Leu Lys Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Asp Asp Arg His Pro Leu Phe Asp Asp Asn Glu Thr Glu Lys Asp Tyr 
        115                 120                 125             
Tyr Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Met Glu 
    130                 135                 140                 
Asn Asp Glu Lys His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Ser Ser 
                165                 170                 175     
Glu Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Leu Val Gln Val Leu 
            180                 185                 190         
Asn Asp Ala Leu Glu Glu Glu His Leu Ser Glu Glu Ala Asn Asp Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Arg Ser Thr Arg Ala Lys Glu 
    210                 215                 220                 
Val Val Lys Leu Phe Gly Ser Glu Lys Lys Glu Ala Cys Ile Ser Ala 
225                 230                 235                 240 
Leu Val Lys Ala Ile Val Gly Leu Lys Gly Asp Leu Lys Lys Val Phe 
                245                 250                 255     
Gly Asp Ala Glu Asp Thr Ala Ile His Phe Ser Lys Asp Asp Tyr Glu 
            260                 265                 270         
Glu Asp Val Glu Ala Ile Arg Asp Leu Ile Pro Asp Asp Ser Ala Asp 
        275                 280                 285             
Leu Phe Glu Ala Ile Lys Ala Leu Tyr Asp Ala Ile Val Leu Gln Gly 
    290                 295                 300                 
Leu Leu Gly Asp Ser Thr Ser Asn Thr Lys Ala Lys Val Ser Ala Ser 
305                 310                 315                 320 
Met Val Ala Arg Tyr Glu Gln His Gln Lys Asp Leu Lys Leu Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu His Leu Ser Glu Lys Tyr Asn Glu Phe Phe Ser 
            340                 345                 350         
Asp Asn Thr Asn Asn Gly Tyr Ser Ala Tyr Ile Glu Gly Gly Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Leu Leu Lys Glu Leu Glu 
    370                 375                 380                 
Asp Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Asn Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Thr Ser Asp Asn Gly Val Ile Pro Tyr Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asp Ala Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Gly Arg Phe Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Lys Asp Gly Val Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Gly Asn Cys Thr Tyr Leu Pro Gly Glu Thr Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Glu Glu Phe Thr Val Leu Asn Glu Leu Asn Asn Val Arg 
        515                 520                 525             
Leu Thr Ile Asp Lys Gly Lys Arg Arg Arg Phe Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Glu Ile Leu Asp Asp Leu Phe Arg Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Val Glu Asp Tyr Leu Lys Arg Glu Tyr Glu Asn Phe Asp Ser 
                565                 570                 575     
Ala Asp Ile Ser Gly Ile Asp Gly Glu Phe Lys Ser Ser Leu Gly Ser 
            580                 585                 590         
Tyr His Asp Phe Cys Lys Ile Val Val Lys Ala Asp Ser Leu Asp Glu 
        595                 600                 605             
Glu Asp His Lys Asp Ile Ile Glu Glu Ile Ile Lys Trp Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Leu Lys Arg Arg Leu Glu Lys Tyr Gly Glu 
625                 630                 635                 640 
Ile Leu Thr Glu Glu Gln Ile Lys Lys Ile Cys Lys Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Ile Arg Asp Ala 
            660                 665                 670         
Glu Thr Gly Lys Ser Ile Ile Asp Val Leu Arg Glu Thr Asp Pro Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Leu Ser Asp Glu Asp Leu Gly Phe Lys 
    690                 695                 700                 
Glu Glu Ile Asp Gln Leu Asn Ala Glu Ala Ala Gly Glu Asn Leu Gln 
705                 710                 715                 720 
Asp Ile Val Asp Asp Leu Pro Gly Ser Pro Ala Ile Lys Arg Gly Ile 
                725                 730                 735     
Asn Gln Ala Ile Arg Ile Val Asp Glu Ile Val Lys Val Met Gly Lys 
            740                 745                 750         
Glu Pro Ser Asn Ile Phe Val Glu Met Ala Arg Glu Glu Gln Gln Ser 
        755                 760                 765             
Ala Arg Gly Arg Arg Ser Thr Lys Arg Arg Leu Lys Arg Leu Gln Glu 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asp Leu Leu Lys Glu Leu Leu Arg Asp 
785                 790                 795                 800 
Asp Asn Asp Leu Arg Asp Asp Arg Leu Phe Leu Tyr Tyr Thr Gln Met 
                805                 810                 815     
Gly Lys Cys Met Tyr Thr Gly Glu Pro Ile Asp Leu Asp Asp Leu Ser 
            820                 825                 830         
Lys Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Val Ser Arg Glu Glu Asn Ala Arg Lys 
    850                 855                 860                 
Thr Asp Asn Leu Pro Ser Pro Glu Ile Arg Arg Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Met Leu Leu Asn Ala Arg Leu Ile Ser Lys Lys Lys Phe Asp 
                885                 890                 895     
Arg Leu Thr Arg Ala Asp Arg Gly Asp Phe Thr Asp Asp Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Thr Leu Leu Glu Gln Arg Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Arg Asn Ala Lys Ile Val Ser Val Lys Ala Asn Leu 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Gly Leu Tyr Lys Cys Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Asn Ala Ile Leu Lys Arg Tyr Pro Ser Leu Ala Ala Asp Tyr Ile Arg 
        995                 1000                1005            
Gly Asp Tyr Arg Lys Asn Asn Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Ala His Phe Tyr Ser Ser Ile Met Asn Ser Phe Thr Ser Asp Glu 
1025                1030                1035                1040
Lys Ile Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Ile Ala Thr Val Arg Lys Val Met Asn Tyr Arg Gln Val Leu Ile Thr 
            1060                1065                1070        
Arg Lys Val Glu Glu Glu Thr Gly Ala Phe Phe Asp Gln Thr Ile Tyr 
        1075                1080                1085            
Ser Lys Gly Ser Ser Lys Lys Leu Ile Pro Leu Lys Lys Asn Leu Asp 
    1090                1095                1100                
Pro Asp Lys Tyr Gly Gly Tyr Ser Ser Glu Lys Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Glu Tyr Asp Ile Glu Lys Arg Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Gln Leu Val Gly Ile Pro Ile Arg Glu Arg Ala Lys Leu Glu 
            1140                1145                1150        
Lys Asn Pro Ser Lys Tyr Leu Glu Lys Lys Gly Tyr Glu Asn Pro Lys 
        1155                1160                1165            
Val Asp Leu Leu Ile Arg Ile Pro Lys Asn Gln Leu Leu Glu Leu Asp 
    1170                1175                1180                
Gly Gly Arg Arg Arg Ile Leu Ala Ala Ala Lys Glu Leu Lys Asn Ala 
1185                1190                1195                1200
Thr Gln Leu Val Leu Pro Ala Glu Glu Tyr Thr Leu Leu Asp Lys Val 
                1205                1210                1215    
Ala Lys Ile Ala Lys Lys Asn Asn Ser Glu Ser Ile Glu Tyr Val Glu 
            1220                1225                1230        
Glu His Leu Ser Glu Phe Asp Glu Leu Leu Glu Ser Leu Ile Asp Tyr 
        1235                1240                1245            
Ser Pro Lys Leu Ala Leu Gln Asp Asn Asn Leu Glu Lys Leu Lys Glu 
    1250                1255                1260                
Ala Phe Glu Gln Leu Asn Leu Ala Asp Lys Lys Asp Val Ala Lys Glu 
1265                1270                1275                1280
Ile Ile Gly Leu Leu His Cys Thr Ala Thr Thr Ala Asn Ala Ala Leu 
                1285                1290                1295    
Lys Ser Leu Gly Gly Ser Lys Asn Arg Met Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Ser Leu Leu Asn Ala Ser Leu Ile His Gln Ser Ile Thr Gly Leu Phe 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 12
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #62

<400> 12
Thr Thr Asp Asp Tyr Ser Ile Gly Leu Asp Met Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Ser Asn Gly Ser Met Ala His Lys Lys Met 
            20                  25                  30          
Pro Thr Leu Gly Asn Thr Asp Lys Lys Ser Ile Gly Lys Pro Thr Leu 
        35                  40                  45              
Gly Ser Arg Leu Phe Asp Ala Ala Glu Thr Ala Ala Glu Ala Arg Ala 
    50                  55                  60                  
His Arg Gly Gln Arg Arg Arg Tyr Val Arg Arg Arg Trp Arg Leu Asp 
65                  70                  75                  80  
Leu Leu Gln Gly Leu Phe Gln Asp Glu Ile Asn Lys Val Asp Ser Glu 
                85                  90                  95      
Phe Phe Ile Arg Leu Arg Gln Ser Arg Leu His Val Glu Asp Arg Ala 
            100                 105                 110         
Asp Tyr Arg Trp Pro Leu Phe Asn Asp Ser Asp Thr Glu Lys Asp Tyr 
        115                 120                 125             
Tyr Lys Arg Phe Pro Thr Ile Tyr His Leu Arg Lys Trp Leu Met Glu 
    130                 135                 140                 
Thr Asp Glu Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Val His Asn 
145                 150                 155                 160 
Ile Val Lys His Arg Gly Asn Phe Leu Arg Glu Lys Asn Leu Ser Ser 
                165                 170                 175     
His Asn Ala Asp Pro Ala Glu Ala Val Arg His Leu Arg Gln Ala Leu 
            180                 185                 190         
Asn Asp Trp Leu Glu Arg Gly Tyr Leu Ala Glu Asn Asp Ser His Ile 
        195                 200                 205             
Leu Asp Ile Leu Thr Asp Ser Glu Arg Arg Ser Asp Gln Ala Lys Glu 
    210                 215                 220                 
Ile Ala Lys Leu Phe Gly Thr Glu Gln Ser Asp Ala Cys Asn Ser Ala 
225                 230                 235                 240 
Leu Ala Lys Ala Ile Val Gly Leu Lys Ala Glu Leu Lys Asn Val Phe 
                245                 250                 255     
Gly Glu Ala Glu Lys Thr Ala Ile His Leu Ser Ser Asp Asp Tyr Glu 
            260                 265                 270         
Asp Asp Val Glu Ala Ile Arg Asp Ala Ile Pro Asp Asp Ser Gly Glu 
        275                 280                 285             
Leu Phe Gln Ala Leu Cys Ala Leu Tyr Ser Ala Tyr Val Leu Gln Gly 
    290                 295                 300                 
Leu Leu Ser Tyr Ala Pro Gly Gln Thr Lys Ala Lys Ile Ser Ala Asn 
305                 310                 315                 320 
Met Val Ala Lys Tyr Asp Gln Tyr Gly Lys Asp Leu Lys Leu Leu Lys 
                325                 330                 335     
Asn Leu Ala Arg Thr Tyr Val Ser Glu Asn Tyr Asp Ala Phe Phe Arg 
            340                 345                 350         
Gly Glu Thr Tyr Glu Gly Ser Ser Asp Tyr Val Gln Gly Tyr Ala Ser 
        355                 360                 365             
Tyr Asp Asp Phe Gln Lys Ser Val Lys Lys Leu Phe Lys Gln Leu Glu 
    370                 375                 380                 
Asp Asp Arg Tyr Met Met Lys Arg Phe Gly Glu Glu Lys Phe Leu Arg 
385                 390                 395                 400 
Arg Leu Lys Thr Ser Asp Asn Gly Ser Ile Tyr Tyr Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys His Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Glu Glu Asp Lys Ile Glu Ser Leu Val Ser Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Glu Asn Gln Arg Phe Ala Trp 
    450                 455                 460                 
Ser Ser Arg Met Lys Asp Ala Val Ile Thr Pro Trp Asn Trp Asp Glu 
465                 470                 475                 480 
Ile Ile Asp Arg Asp Lys Ser Ala Glu Glu Phe Ile Leu Arg Met Thr 
                485                 490                 495     
Gly Asp Cys Thr Tyr Leu Gln Gly Glu Pro Val Leu Pro Lys Cys Ser 
            500                 505                 510         
Leu Leu Tyr Glu Glu Phe Cys Val Leu Asn Glu Leu Asn Gly Ala Arg 
        515                 520                 525             
Trp Thr Ile Asp Glu Asp Asp Glu Arg Arg Phe Asp Ala Ala Gln Arg 
    530                 535                 540                 
Glu Gly Ile Leu His Asp Leu Phe Arg Lys Thr Arg Lys Val Thr Tyr 
545                 550                 555                 560 
Lys Lys Val Ala Asp Trp Leu Glu Arg Glu Tyr Glu Asn Phe Ala Asn 
                565                 570                 575     
Ala His Val Arg Gly Gly Gln Gly Glu Phe Glu Ser Lys Leu Gly Ser 
            580                 585                 590         
Tyr Ile Phe Phe Cys Lys Asp Val Phe Lys Thr Asp Ser Leu Asp Glu 
        595                 600                 605             
Ala Asp Tyr Lys Pro Met Ile Glu Gln Ile Ile Leu Trp Asn Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Asp Ile Leu Lys Arg Lys Ile Glu Ala Tyr Gly Glu 
625                 630                 635                 640 
His Leu Thr Asp Glu Gln Ile Lys Val Ile Cys Lys Lys Arg Phe Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Ile Lys Asp Ala 
            660                 665                 670         
Asp Thr Gly Arg Ser Ile Ile Asp Val Leu Arg Glu Gly Asn Pro Asn 
        675                 680                 685             
Ser Arg Thr Phe Met Glu Val Leu Arg Asp Glu Asp Leu Gly Phe Gln 
    690                 695                 700                 
Lys Lys Ile Asp Asp Phe Asn Lys Asp Tyr Ala Gly Glu Asn Leu Met 
705                 710                 715                 720 
Asp Ile Val Asn Asp Leu Pro Gly Ser Pro Ala Ile Arg Arg Ser Ile 
                725                 730                 735     
Asn Gln Ala Ile Arg Ile Val Asp Glu Ile Val Ser Ile Ala Gly Lys 
            740                 745                 750         
Glu Pro Ser Asn Ile Phe Ile Glu Val Thr Arg Glu Glu Asp Asp Pro 
        755                 760                 765             
Arg Lys Lys Gly Arg Arg Thr Lys Arg Arg Tyr Glu Asp Ile Lys Asn 
    770                 775                 780                 
Ala Leu Glu Asn Phe Lys Thr Asp Leu Trp Lys Glu Leu Leu Gly Lys 
785                 790                 795                 800 
Asp Ser Asn Asn Leu Asp Glu Arg Leu Ser Leu Tyr Phe Met Gln Arg 
                805                 810                 815     
Gly Lys Cys Met Tyr Ser Gly Arg Pro Ile Lys Leu Glu Glu Leu His 
            820                 825                 830         
Lys Tyr Glu Val Asp His Ile Ile Pro Arg Ser Tyr Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Glu Asn Lys Val Leu Val Cys Arg Glu Glu Asn Gln Arg Lys 
    850                 855                 860                 
Thr Asp Glu Leu Leu Asp Pro Ala Ile Arg Arg Lys Met Gly Asp Tyr 
865                 870                 875                 880 
Trp Arg Met Leu His Lys Ala Lys Leu Ile Gly Asp Lys Lys Phe Asn 
                885                 890                 895     
Asn Leu Phe Arg Arg Asp Arg Gly Asp Ile Asp Asp Lys Thr Leu Lys 
            900                 905                 910         
Gly Phe Val Ala Arg Gln Leu Val Glu Thr Ser Gln Met Val Lys Leu 
        915                 920                 925             
Val Gln Thr Leu Leu Glu Ala Arg Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Lys Asn Ala Lys Ile Val Pro Val Lys Ala Asn Ile 
945                 950                 955                 960 
Ser His Asp Leu Arg Glu Ala Ala Gly Leu Val Lys Cys Arg Glu Ala 
                965                 970                 975     
Asn Asp Phe His His Ala His Asp Ala Tyr Leu Ala Cys Arg Val Gly 
            980                 985                 990         
Leu Phe Ile Gln Lys Arg His Pro Ser Val Tyr Ala Asn His Val Met 
        995                 1000                1005            
Arg Asp Tyr Ala Arg Lys Gln Ala Arg Glu Tyr Asn Lys Thr His Ser 
    1010                1015                1020                
Met Ala His Thr Lys Gly Phe Ile Val Asn Ser Phe Met Ser Ser Glu 
1025                1030                1035                1040
Phe Asp Lys Asp Glu Thr Gly Glu Ile Phe Trp Asp Ala Glu Ala Glu 
                1045                1050                1055    
Val Glu Asp Ile Arg Arg Val Leu Asn Tyr Arg Gln Cys Phe Ile Ser 
            1060                1065                1070        
Arg Met Pro Val Glu Asp Thr Gly Ala Phe Trp Asp Gln Thr Ile Tyr 
        1075                1080                1085            
Ser Pro Arg Ser Asn Lys Lys Leu Leu Pro Leu Lys Arg Asp Leu Asp 
    1090                1095                1100                
Pro Asp Lys Tyr Gly Ser Tyr Ser Arg Glu Gln Phe Ala Tyr Ser Val 
1105                1110                1115                1120
Phe Phe Ile Tyr Asp Ile Glu Ala Arg Asp Ala Lys Lys Ser Lys Thr 
                1125                1130                1135    
Val Leu Gln Phe Ala Gln Val Pro Val Arg Val Thr Ala Ala Ile Ala 
            1140                1145                1150        
Lys Asp Pro Ser Lys Tyr Ala Ser Lys Leu Gly Gln Glu Asn Leu Glu 
        1155                1160                1165            
Phe Thr Arg Ile Ile Arg Ile Leu Lys Lys Gln Leu Leu Glu Leu Glu 
    1170                1175                1180                
Asn Gly Asp Arg Leu Ile Ile Thr Gly Ala Lys Glu Val Arg Asn Ala 
1185                1190                1195                1200
Thr Glu Phe Ala Phe Ser Leu Glu Glu Thr Thr Leu Val Glu Arg Cys 
                1205                1210                1215    
Ser Gln Ile Ala Lys Glu Gly Asn Pro Glu Asp Ile Glu Tyr Val Glu 
            1220                1225                1230        
Gln Leu Ser Ser Gln Leu Asn Glu Leu Phe Ser Ser Leu Ile Glu Arg 
        1235                1240                1245            
Gly Pro Lys Leu Gly Val Arg Leu His Ala Gln Leu Lys Leu Asp Gln 
    1250                1255                1260                
Tyr Leu Ser Gln Phe Asp Asp Ala Ala Leu Arg Asp Lys Ala Lys Val 
1265                1270                1275                1280
Ile Ile Gly Leu Ile Ser Ile Thr Asn Gly Ala Thr Asn Thr Ala Leu 
                1285                1290                1295    
Ser Ser Leu Gly Gly Ser Lys Asn Arg Met Arg Tyr Thr His Asn Lys 
            1300                1305                1310        
Leu Leu Asn Asn Ala Ser Leu Ile Asp Gln Ser Val Thr Gly Met Phe 
        1315                1320                1325            
Glu Arg Arg Thr His Ile Gly Leu Leu Gly Glu Asp 
    1330                1335                1340

<210> 13
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #64

<400> 13
Met Lys Lys Glu Tyr Tyr Ile Gly Leu Asp Met Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Cys Lys Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Thr Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Tyr Arg Thr Ser Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ser Glu Glu Ile Ser Lys Val Asp Pro Thr 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Glu Asp Arg Tyr Pro Leu Phe Val Asp Asn Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Met Glu 
    130                 135                 140                 
Asn Asp Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asp Ser 
                165                 170                 175     
Glu Asn Thr Asp Leu Gly Glu Val Phe Lys Glu Leu Val Lys Val Phe 
            180                 185                 190         
Asn Asp Glu Leu Glu Ile Glu Leu Leu Ser Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Lys Ser Ala Lys Ala Lys Glu 
    210                 215                 220                 
Ile Ala Lys Leu Phe Gly Ser Glu Lys Lys Gln Ala Cys Ile Lys Ala 
225                 230                 235                 240 
Leu Val Lys Leu Ile Val Gly Leu Thr Gly Asn Leu Ser Glu Ile Phe 
                245                 250                 255     
Gly Asp Ser Glu Glu Thr Ser Ile Asn Phe Ser Glu Ser Asn Tyr Glu 
            260                 265                 270         
Glu Asn Met Glu Ala Ile Glu Glu Ile Leu Pro Asp Glu Phe Ser Glu 
        275                 280                 285             
Leu Ile Glu Ala Ile Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asp Leu Gln Gln Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Thr His Cys Pro Glu Glu Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Lys Asn Gly Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Glu Glu Leu Glu 
    370                 375                 380                 
Lys Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Gln Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Ser Ile Phe Lys Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Gly Asn Gly Ser Asn Ala Trp 
    450                 455                 460                 
Met Val Arg Lys Glu Asp Gly Lys Ile Tyr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Val Val Asp Val Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Glu Lys Ile Lys Lys Arg Arg Phe Ser Val Glu Leu Lys 
    530                 535                 540                 
Gln Glu Ile Tyr Asn Asp Leu Phe Cys Arg Asn Arg Lys Val Thr Gly 
545                 550                 555                 560 
Lys Lys Ile Lys Asp Tyr Leu Lys Arg Glu Tyr Asn Glu Ile Asp Glu 
                565                 570                 575     
Ala Glu Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ser Leu Arg Ser 
            580                 585                 590         
Tyr His Asp Phe Lys Lys Ile Val Gly Lys Gly Asn Ser Leu Asp Glu 
        595                 600                 605             
Glu Asp Tyr Lys Glu Ile Ile Glu Glu Ile Ile Lys Trp Ile Thr Ile 
    610                 615                 620                 
Phe Asp Asp Lys Lys Met Leu Lys Arg Arg Leu Lys Lys Tyr Pro Glu 
625                 630                 635                 640 
Ile Leu Ser Glu Glu Gln Ile Lys Lys Ile Cys Lys Leu Lys Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Thr Gly Val Arg Asp Lys 
            660                 665                 670         
Glu Thr Gly Glu Ser Ile Ile Asn Ala Leu Trp Glu Thr Asp Ser Ser 
        675                 680                 685             
Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Lys Asp Tyr Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Gln Ile Asn Ala Glu Ala Ala Gly Glu Asn Ser Tyr 
705                 710                 715                 720 
Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Ala Ile 
                725                 730                 735     
Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Val Lys Val Met Gly His 
            740                 745                 750         
Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Glu Lys Glu Lys Asn 
        755                 760                 765             
Gly Lys Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu Tyr Lys 
    770                 775                 780                 
Ser Cys Lys Asn Asp Ala Ser Asp Leu Leu Lys Glu Leu Gly Arg Asp 
785                 790                 795                 800 
Asp Asn Glu Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr Gln Met 
                805                 810                 815     
Gly Lys Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Asp Asp Leu Phe 
            820                 825                 830         
Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Val Arg Lys Glu Val Asn Ala Lys Lys 
    850                 855                 860                 
Ser Asp Asn Phe Pro Ser Pro Glu Ile Arg Glu Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Met Leu Leu Lys Lys Gly Phe Ile Ser Lys Lys Lys Tyr Asp 
                885                 890                 895     
Arg Leu Thr Arg Thr Thr Arg Gly Glu Phe Thr Asp Asp Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala 
        915                 920                 925             
Val Ala Glu Leu Leu Lys Gln Leu Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Arg Asp Ser Glu Ile Val Tyr Val Lys Ala Asn Leu 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Asp Leu Ile Lys Cys Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val Gly 
            980                 985                 990         
Asn Val Tyr His Val Lys Tyr Thr Asn Asn Ala Ala Lys Phe Ile Lys 
        995                 1000                1005            
Gly Asn Arg Gly Arg Asn Tyr Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Met His Phe Tyr Ser Asn Ile Lys Lys Met Phe Thr Ser Asp Val 
1025                1030                1035                1040
Lys Ser Ala Asp Lys Asn Gly Glu Ile Val Trp Asp Lys Asp Lys Ser 
                1045                1050                1055    
Ile Ala Thr Val Arg Lys Ile Met Asn Lys Asn Asn Ile Leu Tyr Thr 
            1060                1065                1070        
Arg Tyr Thr Tyr Glu Glu Lys Gly Gly Leu Phe Asn Gln Thr Ile Leu 
        1075                1080                1085            
Lys Lys Gly Asn Ser Gln Lys Leu Ile Pro Leu Lys Lys Asn Leu Asp 
    1090                1095                1100                
Pro Glu Lys Tyr Gly Gly Tyr Asn Ser Pro Thr Ala Ala Tyr Phe Ala 
1105                1110                1115                1120
Leu Val Glu Tyr Asp Ile Glu Glu Gly Lys Lys Arg Lys Arg Lys Thr 
                1125                1130                1135    
Thr Lys Gln Leu Val Ala Ile Pro Ile Tyr Glu Lys Ala Lys Leu Glu 
            1140                1145                1150        
Glu Asn Pro Ile Lys Tyr Leu Glu Glu Arg Gly Leu Lys Asn Pro Ala 
        1155                1160                1165            
Val Asp Leu Leu Ile Lys Ile Lys Lys Asn Ser Leu Phe Glu Met Asp 
    1170                1175                1180                
Gly Phe Arg Met Arg Leu Ser Gly Ala Thr Ile Val Phe Lys Asn Ala 
1185                1190                1195                1200
Val Gln Leu Val Leu Asp Glu Glu His Tyr Glu Ile Leu Lys Lys Val 
                1205                1210                1215    
Ala Lys Val Leu Glu Lys Asn Glu Asn Gln Thr Ile Gly Tyr Thr Glu 
            1220                1225                1230        
Glu Leu Leu Ser Leu Tyr Asp Glu Leu Leu Glu Lys Leu Lys Asp Tyr 
        1235                1240                1245            
Lys Asn Arg Leu Ala Asn Gln Ala Lys Thr Leu Glu Lys Gly Lys Glu 
    1250                1255                1260                
Lys Phe Glu Lys Leu Asn Leu Glu Asp Lys Ala Glu Val Ile Asn Glu 
1265                1270                1275                1280
Ile Ile Asn Leu Phe His Cys Asn Ala Thr Thr Ala Asn Asn Ala Leu 
                1285                1290                1295    
Lys Leu Ile Gly Gly Ser Lys Asn Arg Met Lys Asn Asn Asn Ile Ser 
            1300                1305                1310        
Lys Ser Asn Lys Val Ser Leu Ile Asn Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Lys Ile Asp Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 14
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #65

<400> 14
Met Lys Lys Glu Tyr Tyr Leu Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Val Lys Arg His Val 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Ile Arg Leu Phe Glu Thr Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Tyr Arg Ser Ser Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Asp 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ser Glu Glu Ile Ala Lys Val Asp Leu Thr 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Glu Glu Lys Tyr Pro Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Ile Glu 
    130                 135                 140                 
Asn Pro Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Ile Glu Gly Asp Ser Asp Asn 
                165                 170                 175     
Lys Asn Thr Asp Leu Ser Glu Val Phe Ser Glu Leu Val Lys Ala Phe 
            180                 185                 190         
Asn Asp Val Ile Glu Tyr Glu Ile Leu Ser Thr Glu Ala Leu Glu Leu 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Thr Ser Lys Ser Ala Lys Ala Lys Glu 
    210                 215                 220                 
Ile Ala Lys Leu Phe Glu Asp Glu Lys Glu Gln Gln Cys Ile Lys Ala 
225                 230                 235                 240 
Leu Ser Lys Leu Ile Val Gly Leu Thr Gly Asn Ile Ser Glu Met Phe 
                245                 250                 255     
His Gln Ser Glu Glu Thr Ser Ile Asn Phe Ser Glu Ser Asn Tyr Glu 
            260                 265                 270         
Glu Asn Met Glu Ala Ile Glu Glu Ile Ile Pro Asp Glu Phe Ser Glu 
        275                 280                 285             
Leu Ile Asp Ala Ile Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Glu 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asn Leu Lys Gln Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Thr Tyr Cys Ser Glu Glu Tyr Lys Glu Phe Phe Ser 
            340                 345                 350         
Ser Asp Glu Lys Asn Gly Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Glu Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Glu Glu Lys Glu 
    370                 375                 380                 
Lys Ala Glu Tyr Leu Leu Ala Glu Ile Glu Asn Glu Thr Leu Leu Pro 
385                 390                 395                 400 
Leu Gln Arg Ser Lys Asp Asn Gly Thr Ile Pro His Gln Val His Leu 
                405                 410                 415     
Gln Glu Leu Lys Lys Ile Leu Glu Asn Ala Ser Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Ile Ser Ile Phe Lys Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Arg Asn Gly Ser Asn Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Glu Asp Gly Arg Ile Tyr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Val Val Asp Val Glu Lys Ser Asn Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Lys 
        515                 520                 525             
Ile Arg Gly Asn Lys Ile Lys Lys Arg Arg Phe Ser Val Glu Leu Lys 
    530                 535                 540                 
Gln Glu Ile Tyr Asn Asp Leu Phe Cys Arg Asn Arg Lys Val Thr Gly 
545                 550                 555                 560 
Lys Lys Ile Leu Asp Tyr Leu Lys Arg Glu Tyr Asn Glu Ile Asp Gln 
                565                 570                 575     
Ala Asp Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ala Leu Ala Ser 
            580                 585                 590         
Tyr His Asp Phe Lys Lys Leu Val Gly Asn Gly Asn Ser Leu Asp Lys 
        595                 600                 605             
Glu Asp Tyr Lys Glu Ile Ile Glu Glu Ile Ile Lys Trp Lys Thr Ile 
    610                 615                 620                 
Tyr Asp Asp Asn Lys Met Leu Arg Arg Met Ile Lys Lys Tyr Pro Glu 
625                 630                 635                 640 
Val Leu Ser Glu Glu Gln Ile Lys Lys Ile Cys Lys Leu Lys Tyr Thr 
                645                 650                 655     
Gly Trp Gly Asn Phe Ser Arg Glu Phe Leu Thr Gly Val Arg Gly Lys 
            660                 665                 670         
Glu Thr Gly Glu Ser Ile Ile Asn Ala Leu Trp Glu Thr Asp Ser Ser 
        675                 680                 685             
Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Lys Asp Tyr Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Asp Gln Ile Asn Ala Glu Ala Ala Gly Glu Asn Ser Tyr 
705                 710                 715                 720 
Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Asn Lys Arg Ala Ile 
                725                 730                 735     
Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Val Lys Val Met Gly His 
            740                 745                 750         
Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Gly Lys Glu Lys Asn 
        755                 760                 765             
Ala Lys Arg Thr Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu Tyr Lys 
    770                 775                 780                 
Ser Cys Lys Asn Asp Thr Arg Asn Leu Met Lys Glu Leu Gly Arg Asp 
785                 790                 795                 800 
Asp Asn Glu Leu Arg Ser Lys Lys Leu Phe Leu Tyr Tyr Thr Gln Met 
                805                 810                 815     
Gly Lys Cys Met Tyr Ser Gly Glu Glu Ile Asp Leu Asp Asp Leu Leu 
            820                 825                 830         
Asn Trp Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Met Val Leu Val Arg Lys Glu Val Asn Ala Lys Lys 
    850                 855                 860                 
Ser Asn Glu Leu Leu Ser Pro Glu Ile Arg Glu Lys Met Lys Pro Tyr 
865                 870                 875                 880 
Trp Lys Met Leu Leu Gln Lys Gly Phe Ile Ser Lys Lys Lys Tyr Asp 
                885                 890                 895     
Arg Leu Thr Arg Thr Thr Arg Gly Glu Phe Thr Asp Glu Glu Leu Ser 
            900                 905                 910         
Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala 
        915                 920                 925             
Val Ala Glu Leu Leu Lys Gln Leu Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Arg Asp Ser Glu Val Val Tyr Val Lys Ala Asn Leu 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Asp Leu Leu Lys Cys Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val Gly 
            980                 985                 990         
Asn Val Tyr His Val Arg Tyr Thr Asn Asn Pro Ala Lys Trp Ile Lys 
        995                 1000                1005            
Gly Asn Arg Asp Ser Asn Tyr Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Lys His Phe Tyr Ser Ser Ile Lys Lys Met Phe Ser Tyr Asp Val 
1025                1030                1035                1040
Lys Asn Ala Asp Lys Asn Gly Glu Ile Val Trp Asn Lys Asp Ser Ser 
                1045                1050                1055    
Ile Asp Gln Ile Arg Lys Ile Met Asn Lys Asn Asn Ile Leu Tyr Thr 
            1060                1065                1070        
Glu Tyr Thr Tyr Cys Glu Lys Gly Gln Leu Phe Asn Glu Thr Leu Leu 
        1075                1080                1085            
Lys Lys Gly Asn Ser Glu Lys Leu Ile Pro Leu Lys Lys Asn Leu Asp 
    1090                1095                1100                
Pro Glu Lys Tyr Gly Gly Tyr Thr Thr Pro Lys Ala Ser Tyr Phe Ala 
1105                1110                1115                1120
Leu Val Glu Phe Asp Ile Glu Glu Gly Lys Lys Arg Lys Arg Lys Thr 
                1125                1130                1135    
His Lys Gln Leu Ile Pro Val Pro Val Tyr Glu Lys Ala Lys Leu Glu 
            1140                1145                1150        
His Asn Ala Val Lys Tyr Leu Glu Glu Arg Gly Leu Ser Asn Val Val 
        1155                1160                1165            
Val Asp Leu Lys Tyr Lys Ile Lys Lys Asn Ser Leu Leu Lys Met Asp 
    1170                1175                1180                
Gly Tyr Arg Met Arg Leu Arg Gly Ala Asn Ile Ile Phe Lys Asn Ala 
1185                1190                1195                1200
Val Gln Leu Val Leu Asp Glu Glu His Tyr Glu Ile Leu Arg Lys Val 
                1205                1210                1215    
Glu Lys Tyr Leu Glu Lys Asn Glu Asn Gln Thr Ile Gly Phe Thr Glu 
            1220                1225                1230        
Glu Leu Leu Ser Leu Tyr Glu Glu Leu Leu Glu Lys Leu Lys Asp Tyr 
        1235                1240                1245            
Lys Asn Arg Pro Ala Asn Gln Met Lys Thr Leu Glu Lys Gly Lys Glu 
    1250                1255                1260                
Ala Phe Glu Ser Ile Asn Leu Glu Asp Lys Ala Glu Val Ile Asn Glu 
1265                1270                1275                1280
Ile Ile Lys Leu Phe Arg Cys Asn Ala Thr Thr Ala Ser Asn Ala Leu 
                1285                1290                1295    
Lys Leu Ile Asn Gly Ser Gln Asn Arg Met Lys Asn Lys Asn Ile Leu 
            1300                1305                1310        
Asn Ser Asn Lys Val Lys Leu Ile Asn Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Lys Ile Asp Leu Val Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 15
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #66

<400> 15
Met Lys Lys Asp Tyr Tyr Ile Gly Leu Asp Met Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Cys Lys Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Glu Thr Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ala Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ser Glu Glu Ile Ser Lys Val Asp Ile Ala 
                85                  90                  95      
Phe Phe Leu Arg Leu Asn Glu Ser Lys Leu Tyr Pro Glu Asp Lys Thr 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Met Gln 
    130                 135                 140                 
Asn Asp Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Phe Glu Gly Asp Leu Asp Ser 
                165                 170                 175     
Glu Val Thr Asp Phe Gly Ala Val Phe Lys Glu Leu Leu Lys Val Ile 
            180                 185                 190         
Asn Asp Glu Leu Glu Ile Ala Leu Leu Ser Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Lys Ser Lys Ser Ala Lys Ser Lys Glu 
    210                 215                 220                 
Ile Val Lys Leu Leu Gly Ser Glu Lys Lys Gln Ala Cys Ile Lys Ala 
225                 230                 235                 240 
Leu Val Lys Leu Ile Val Gly Leu Thr Val Lys Leu Ser Glu Ile Phe 
                245                 250                 255     
Gly Asp Gly Glu Lys Ser Ser Ile Ser Phe Ala Glu Ser Asn Tyr Glu 
            260                 265                 270         
Glu Asn Val Glu Thr Val Glu Glu Thr Leu Pro Glu Arg Phe Ser Glu 
        275                 280                 285             
Leu Ile Glu Ala Ile Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Thr Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asp Leu Gln Gln Leu Lys 
                325                 330                 335     
Ala Leu Val Lys Thr His Cys Pro Glu Glu Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Leu Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Thr Leu Glu Glu Leu Glu 
    370                 375                 380                 
Lys Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Ser Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Ile Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Arg Gly Asn Gly Glu Asn Ala Trp 
    450                 455                 460                 
Met Val Arg Lys Glu Asp Gly Lys Ile Tyr Pro Trp Asn Phe Asp Asn 
465                 470                 475                 480 
Val Val Asp Val Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Glu Lys Ile Lys Lys Arg Arg Phe Ser Val Glu Leu Lys 
    530                 535                 540                 
Gln Ala Ile Tyr Asn Asp Leu Phe Cys Arg Arg Arg Lys Val Thr Gly 
545                 550                 555                 560 
Lys Lys Leu Lys Asp Tyr Leu Lys Arg Glu Gly Gly Thr Gly Lys Glu 
                565                 570                 575     
Ala Glu Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ser Leu Arg Ala 
            580                 585                 590         
Tyr His Asp Phe Lys Lys Ile Val Gly Lys Gly Asn Ser Leu Asp Glu 
        595                 600                 605             
Glu Asp Asn Gln Asn Ile Lys Glu Glu Ile Ile Lys Ser Ile Thr Ile 
    610                 615                 620                 
Phe Asp Asp Lys Lys Met Leu Lys Lys Arg Leu Lys Lys Tyr Pro Glu 
625                 630                 635                 640 
Ile Leu Ser Glu Glu Gln Ile Lys Lys Ile Cys Lys Leu Lys Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Thr Glu Val Arg Asp Lys 
            660                 665                 670         
Glu Thr Gly Glu Asn Ile Ile Asn Ala Leu Trp Glu Thr Asp Ser Ser 
        675                 680                 685             
Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Lys Asp Tyr Ser Phe Gln 
    690                 695                 700                 
Glu Glu Ile Glu Gln Ile Asn Ala Glu Ala Ala Gly Glu Ser Ser Tyr 
705                 710                 715                 720 
Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Ala Ile 
                725                 730                 735     
Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Val Lys Val Met Gly His 
            740                 745                 750         
Glu Pro Lys Arg Val Phe Val Glu Met Ala Arg Glu Lys Gly Lys Asn 
        755                 760                 765             
Gly Lys Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu Tyr Lys 
    770                 775                 780                 
Ser Cys Lys Asn Asp Ala Pro Asp Leu Trp Lys Glu Leu Gly Arg Asp 
785                 790                 795                 800 
Asp Asn Glu Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr Gln Met 
                805                 810                 815     
Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp Leu Phe 
            820                 825                 830         
Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Val Lys Lys Glu Val Asn Ser Lys Lys 
    850                 855                 860                 
Ser Asp Asn Tyr Pro Ser Pro Glu Ile Arg Glu Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Met Leu Leu Lys Lys Gly Phe Ile Ser Lys Lys Lys Tyr Asp 
                885                 890                 895     
Arg Leu Thr Arg Thr Thr Arg Gly Glu Phe Thr Asp Asp Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala 
        915                 920                 925             
Val Ala Glu Leu Leu Lys Gln Leu Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Arg Asp Thr Glu Ile Val Tyr Val Lys Ala Asn Leu 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Asp Phe Ile Lys Cys Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val Gly 
            980                 985                 990         
Asn Val Tyr His Val Lys Tyr Thr Lys Asn Ala Ala Lys Phe Ile Lys 
        995                 1000                1005            
Gly Asn Pro Gly Arg Asn Tyr Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Met His Phe Tyr Ser Asn Leu Lys Lys Met Phe Thr Ser Asp Val 
1025                1030                1035                1040
Pro Ser Ala Asp Arg Asn Gly Glu Ile Val Trp Asp Lys Asp Gly Ser 
                1045                1050                1055    
Ile Ala Thr Val Arg Lys Ile Met Asn Lys Asn Asn Ile Leu Tyr Thr 
            1060                1065                1070        
Arg Tyr Ser Tyr Glu Glu Lys Gly Gly Leu Phe Asp Gln Gln Ile Leu 
        1075                1080                1085            
Lys Lys Gly Asn Gly Gln Ala Leu Ile Pro Leu Lys Lys Arg Leu Asp 
    1090                1095                1100                
Ile Glu Lys Tyr Gly Gly Tyr Asn Lys Pro Thr Ala Ala Tyr Phe Ala 
1105                1110                1115                1120
Leu Val Glu Tyr Asp Ile Glu Glu Gly Lys Lys Gly Lys Lys Lys Thr 
                1125                1130                1135    
Thr Lys Gln Leu Val Ala Ile Pro Ile Tyr Glu Lys Ala Lys Leu Glu 
            1140                1145                1150        
Glu Asn Pro Ile Lys Tyr Leu Glu Glu Arg Gly Leu Lys Asn Pro Ala 
        1155                1160                1165            
Val Asp Leu Leu Ile Lys Ile Lys Lys Asn Ser Leu Phe Glu Val Asp 
    1170                1175                1180                
Gly Phe Arg Met His Leu Ser Gly Arg Thr Ile Val Phe Lys Ser Ala 
1185                1190                1195                1200
Val Gln Leu Val Leu Asp Glu Glu Asn Glu Ala Ile Leu Lys Lys Val 
                1205                1210                1215    
Ala Lys Val Leu Glu Lys His Lys Asn Gln Lys Ile Gly Tyr Thr Glu 
            1220                1225                1230        
Glu Leu Leu Ser Leu Tyr Asp Ala Leu Leu Glu Lys Leu Lys Asp Tyr 
        1235                1240                1245            
Lys Ile Arg Leu Ala Thr Gln Ala Lys Thr Leu Glu Asn Gly Lys Glu 
    1250                1255                1260                
Lys Phe Glu Lys Leu Ser Leu Glu Asp Lys Cys Gln Val Ile Asn Glu 
1265                1270                1275                1280
Ile Leu His Leu Phe Gln Cys Asn Ala Thr Thr Ala Asn Asn Ala Leu 
                1285                1290                1295    
Ser Leu Ile Gly Gly Ala Lys Asn Ile Met Thr Asn Asn Asn Ile Ser 
            1300                1305                1310        
Lys Cys Asn Lys Val Ser Leu Ile Asn Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Lys Ile Asp Leu Lys Lys Ile Gly Glu Asp 
    1330                1335                1340

<210> 16
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #67

<400> 16
Met Lys Lys Glu Tyr Tyr Val Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Ile Cys Arg Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Glu Thr Ala Asn Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ala Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Lys Val Asp Ile Gly 
                85                  90                  95      
Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro Glu Asp Lys Pro 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Asp Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Gln 
    130                 135                 140                 
Asn Glu Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Phe Glu Gly Asp Ile Ser Ser 
                165                 170                 175     
Glu Val Thr Asp Phe Gly Ala Ala Phe Ser Glu Phe Leu Lys Val Ile 
            180                 185                 190         
Asn Asp Glu Leu Glu Ile Ser Leu Leu Asp Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Lys Ser Lys Ser Ala Lys Lys Thr Glu 
    210                 215                 220                 
Leu Val Lys Leu Leu Ala Ala Glu Lys Lys Gln Lys Cys Glu Lys Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Ser Gly Cys Thr Val Lys Leu Ser Asp Ile Phe 
                245                 250                 255     
Gly Asp Gly Glu Arg Ser Lys Ile Ser Phe Ala Asp Ser Asn Tyr Glu 
            260                 265                 270         
Glu Tyr Ile Ala Thr Val Glu Glu Thr Leu Ala Glu Arg Phe Leu Glu 
        275                 280                 285             
Leu Ile Glu Ala Ala Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Thr Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Arg Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Ala Leu Val Lys Thr His Leu Pro Glu Glu Tyr Lys Asp Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Leu Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Lys Glu Asp Phe Tyr Ser Phe Leu Lys Lys Asn Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Ser Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Ile Phe Lys Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Lys Gly Asn Gly Glu Asn Ala Trp 
    450                 455                 460                 
Ala Val Arg Lys Gln Asp Gly Lys Ile Tyr Pro Trp Asn Phe Glu Asn 
465                 470                 475                 480 
Val Ile Asp Val Glu Glu Ser Ala Glu Lys Phe Ile Arg Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asp Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Glu Lys Ile Lys Lys Arg Arg Phe Ser Val Glu Leu Lys 
    530                 535                 540                 
Gln Ala Val Tyr Glu Asp Val Phe Cys Arg Tyr Arg Lys Val Thr Gly 
545                 550                 555                 560 
Lys Lys Leu Lys Asp Tyr Leu Ile Arg Glu Gly Ile Thr Gly Lys Glu 
                565                 570                 575     
Ala Glu Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ser Leu Arg Ala 
            580                 585                 590         
Tyr His Asp Phe Lys Gln Ile Val Gly Lys Gly Lys Leu Leu Asp Val 
        595                 600                 605             
Glu Leu Thr Gln Asn Glu Lys Glu Asp Ile Ile Lys Asn Ile Thr Ile 
    610                 615                 620                 
Phe Asp Asp Lys Lys Met Leu Lys Lys Arg Leu Lys Lys Tyr Pro Glu 
625                 630                 635                 640 
Ile Leu Ser Glu Lys Gln Ile Lys Lys Ile Cys Ala Leu Lys Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Glu Glu Val Gln Ala Lys 
            660                 665                 670         
Glu Thr Gly Glu Asn Ile Ile Ser Ala Leu Trp Glu Thr Asp Ser Ser 
        675                 680                 685             
Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Lys Asp Tyr Gly Phe Gln 
    690                 695                 700                 
Glu Glu Ile Glu Gln Leu Asn Ala Glu Ala Ala Gly Lys Thr Ser Tyr 
705                 710                 715                 720 
Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Pro Ile 
                725                 730                 735     
Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Glu Lys Val Met Gly Ser 
            740                 745                 750         
Lys Pro Lys Arg Val Phe Val Glu Met Ala Arg Glu Lys Gly Glu Asn 
        755                 760                 765             
Gly Arg Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu Tyr Lys 
    770                 775                 780                 
Ser Cys Lys Lys Glu Ala Pro Asp Leu Trp Lys Glu Leu Gly His Asp 
785                 790                 795                 800 
Asp His Lys Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr Gln Met 
                805                 810                 815     
Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp Leu Phe 
            820                 825                 830         
Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Met Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Val Lys Arg Glu Leu Asn Ala Lys Lys 
    850                 855                 860                 
Thr Asp Asn Tyr Pro Ser Ala Glu Ile Arg Glu Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Asp Met Leu Leu Lys Lys Gly Phe Ile Ser Lys Glu Lys Tyr Asp 
                885                 890                 895     
Arg Leu Thr Arg Ser Thr Arg Gly Glu Phe Thr Glu Asn Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala 
        915                 920                 925             
Val Ala Glu Leu Leu Lys Gln Ala Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Lys Glu Thr Glu Ile Val Tyr Val Lys Ala Asn Thr 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Asp Phe Ile Lys Val Arg Asp Val 
                965                 970                 975     
Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val Gly 
            980                 985                 990         
Asn Thr Tyr Tyr Val Lys Phe Thr Lys Asn Ala Ala Trp Phe Ile Lys 
        995                 1000                1005            
Gly Asn Pro Gly Arg Thr Tyr Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Leu His Phe Tyr Ser Asn Leu Lys Lys Met Phe Thr Ser Asp Val 
1025                1030                1035                1040
Ala Ser Ala Asp Arg Asn Gly Glu Ile Ala Trp Gly Lys Asp Gly Thr 
                1045                1050                1055    
Ile Val Thr Val Arg Lys Val Met Asn Lys Asn Asn Ile Leu Phe Thr 
            1060                1065                1070        
Arg Arg Ser Tyr Glu Val Lys Gly Gly Leu Phe Asp Gln Gln Ile Met 
        1075                1080                1085            
Lys Lys Gly Lys Gly Gln Ala Leu Ile Pro Val Lys Glu Arg Leu Ser 
    1090                1095                1100                
Ile Glu Lys Tyr Gly Gly Tyr Asn Lys Ala Thr Gly Ala Tyr Phe Met 
1105                1110                1115                1120
Leu Val Glu Ser Asp Ile Glu Glu Gly Lys Lys Gly Lys Lys Lys Thr 
                1125                1130                1135    
Thr Lys Glu Leu Val Ala Ile Pro Leu Tyr Glu Lys Ala Lys Leu Glu 
            1140                1145                1150        
Asp Asn Ala Ile Arg Tyr Leu Glu Glu Arg Gly Leu Lys Asn Pro Lys 
        1155                1160                1165            
Val Asp Leu Leu Ile Lys Ile Lys Lys Asp Ser Leu Phe Glu Val Asp 
    1170                1175                1180                
Gly Phe Arg Met His Leu Ser Gly Arg Thr Leu Ile Phe Lys Cys Ala 
1185                1190                1195                1200
Asn Gln Leu Val Leu Asp Glu Glu Asn Glu Lys Ile Leu Lys Lys Val 
                1205                1210                1215    
Ala Lys Val Leu Glu Arg His Lys Asn Ile Lys Ile Gly Tyr Thr Glu 
            1220                1225                1230        
Glu Met Leu Ile Lys Tyr Glu Ala Phe Leu Asp Lys Leu Lys Asn Tyr 
        1235                1240                1245            
Asn Ile Arg Leu Ser Thr Gln Ala Lys Thr Leu Glu Asn Gly Lys Glu 
    1250                1255                1260                
Lys Phe Glu Lys Leu Ser Leu Glu Asp Lys Cys Gln Val Ile Asn Glu 
1265                1270                1275                1280
Ile Leu His Leu Phe Gln Cys Gln Ser Val Ala Ala Asn Asn Ala Leu 
                1285                1290                1295    
Ser Met Ile Gly Gly Ala Ala Asn Ile Leu Val Asn Asn Asn Ile Ser 
            1300                1305                1310        
Lys Cys Asn Lys Val Ser Ile Ile Asn Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Gln Glu Ile Asp Leu Lys Lys Ile Gly Glu Asp 
    1330                1335                1340

<210> 17
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #68

<400> 17
Met Lys Lys Glu Tyr Tyr Val Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Ser Tyr His Ile Cys Arg Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ala His Gly Lys Ala Leu Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Glu Thr Ala Asn Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ser Arg Arg Arg Leu Asp Arg Arg Lys Arg Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Lys Val Asp Pro Gly 
                85                  90                  95      
Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro Glu Asp Lys Pro 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Gln 
    130                 135                 140                 
Asp Glu Glu Pro Pro Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Met Ile Lys His Arg Gly His Phe Leu Phe Ala Gly Asp Ile Ser Asp 
                165                 170                 175     
Glu Val Thr Asp Phe Gly Ala Ala Phe Ser Glu Phe Leu Lys Ile Ile 
            180                 185                 190         
Lys Asp Glu Leu Asp Ile Ser Leu Leu Asp Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Leu Thr Arg Ser Ala Lys Lys Thr Glu 
    210                 215                 220                 
Leu Val Lys Gly Leu Lys Ala Glu Lys Lys Asn Gly Cys Glu Lys Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Ser Gly Cys Thr Val Lys Leu Ser Asp Ile Phe 
                245                 250                 255     
Gly Asp Gly Glu Arg Pro Lys Ile Ser Phe Ala Asp Ser Ser Tyr Glu 
            260                 265                 270         
Asp Tyr Ala Ala Val Val Glu Asp Glu Leu Gly Glu Arg Phe Ile Glu 
        275                 280                 285             
Ile Ile Glu Ala Ala Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Ser Val Ser Glu Ala Lys Val Arg Thr Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Arg Asp Leu Lys Tyr Leu Lys 
                325                 330                 335     
Ala Leu Val Lys Thr Tyr Leu Pro Glu Glu Tyr Lys Asp Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Leu Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Lys Glu Asp Phe Tyr Asp Phe Leu Lys Lys Asn Ile Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Leu Glu Lys Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Tyr Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Ile Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Lys Gly Asn Gly Glu Phe Ala Trp 
    450                 455                 460                 
Ala Val Arg Lys Ser Asn Glu Lys Ile Tyr Pro Trp Asn Phe Glu Asn 
465                 470                 475                 480 
Val Ile Asp Val Glu Ala Ser Ala Glu Lys Phe Ile Arg Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asp Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Glu Lys Ile Lys Lys Arg Arg Phe Ser Val Glu Leu Lys 
    530                 535                 540                 
Gln Glu Ile Tyr Glu Asp Val Phe Cys Arg Tyr Arg Lys Val Thr Ala 
545                 550                 555                 560 
Lys Lys Leu Lys Asp Tyr Leu Ile Arg Glu Gly Ile Thr Gly Lys Asp 
                565                 570                 575     
Ala Glu Ile Ser Gly Ile Asp Gly Asp Phe Lys Gly Ser Leu Arg Ala 
            580                 585                 590         
Tyr His Asp Phe Lys Glu Ile Leu Gly Lys Gly Lys Leu Leu Asp Val 
        595                 600                 605             
Glu Leu Thr Gln Asn Glu Lys Glu Asp Ile Ile Leu Asn Ile Thr Leu 
    610                 615                 620                 
Phe Asp Asp Lys Lys Leu Leu Lys Lys Arg Leu Lys Lys Tyr Pro Gln 
625                 630                 635                 640 
Ile Leu Thr Glu Lys Gln Ile Lys Lys Ile Cys Ala Leu Asn Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Glu Glu Val Gln Ala Pro 
            660                 665                 670         
Glu Thr Gly Glu Asn Ile Ile Thr Ala Leu Trp Glu Thr Asp Pro Ser 
        675                 680                 685             
Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Glu Asp Tyr Gly Phe Gln 
    690                 695                 700                 
Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Lys Thr Ser Tyr 
705                 710                 715                 720 
Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Gln Ile 
                725                 730                 735     
Trp Gln Thr Leu Gln Ile Val Lys Glu Ile Glu Lys Val Met Gly Glu 
            740                 745                 750         
Lys Pro Lys Arg Val Phe Val Glu Met Ala Arg Glu Lys Gln Glu Asn 
        755                 760                 765             
Lys Arg Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Asp Leu Tyr Lys 
    770                 775                 780                 
Ser Cys Lys Asn Glu Glu Pro Asp Trp Trp Lys Glu Leu Gly His Glu 
785                 790                 795                 800 
Glu His Lys Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr Gln Lys 
                805                 810                 815     
Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp Leu Trp 
            820                 825                 830         
Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Met Asp Asp 
        835                 840                 845             
Ser Leu Asn Asn Arg Val Leu Val Lys Arg Glu Tyr Asn Ala Lys Lys 
    850                 855                 860                 
Thr Asp Asn Tyr Pro Ser Ala Glu Ile Arg Gln Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Lys Ser Leu Leu Thr Gly Gly Phe Ile Ser Lys Glu Lys Tyr Asp 
                885                 890                 895     
Arg Leu Ile Arg Ser Thr Arg Gly Glu Phe Thr Ala Asn Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Glu Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala 
        915                 920                 925             
Val Ala Glu Ile Leu Lys Gln Val Phe Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Lys Glu Thr Glu Ile Val Tyr Val Lys Ala Lys Thr 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Asp Phe Ile Lys Val Arg Asp Val 
                965                 970                 975     
Asn Asp Phe His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val Gly 
            980                 985                 990         
Asn Thr Tyr Tyr Val Lys Phe Thr Lys Asn Ala Ala Trp Phe Ile Lys 
        995                 1000                1005            
Glu Asn Pro Gly Arg Thr Tyr Ala Arg Glu Glu Lys Lys Ala Asn Ala 
    1010                1015                1020                
Lys Leu His Phe Tyr Ser Asn Leu Lys Lys Met Phe Thr Ser Asp Val 
1025                1030                1035                1040
Ala Val Ala Asp Arg Asn Gly Glu Ile Ala Trp Gly Lys Ser Gly Thr 
                1045                1050                1055    
Ile Val Thr Val Arg Lys Met Met Asn Lys Asn Asn Ile Leu Phe Thr 
            1060                1065                1070        
Arg Arg Ser Tyr Glu Val Lys Gly Gly Leu Phe Asp Gln Gln Ile Met 
        1075                1080                1085            
Lys Lys Gly Lys Gly Gln Val Tyr Ile Pro Val Lys Glu Arg Leu Ser 
    1090                1095                1100                
Ile Glu Lys Tyr Gly Gly Tyr Asn Lys Ala Thr Gly Ala Tyr Phe Met 
1105                1110                1115                1120
Leu Val Glu Ser Asp Ile Glu Glu Gly Lys Lys Gly Lys Lys Lys Arg 
                1125                1130                1135    
Thr Ile Glu Phe Val Pro Ile Pro Leu Tyr Glu Lys Ala Lys Leu Glu 
            1140                1145                1150        
Ala Asn Glu Ala Arg Phe Leu Glu Glu Arg Gly Leu Lys Glu Pro Lys 
        1155                1160                1165            
Val Asp Ile Leu Ile Lys Ile Lys Lys Asp Thr Leu Phe Glu Val Asp 
    1170                1175                1180                
Gly Phe Lys Met His Leu Ser Gly Arg Thr Leu Ile Phe Lys Cys Ala 
1185                1190                1195                1200
Asn Gln Leu Ile Leu Asp Glu Glu Asn Glu Lys Ile Leu Lys Lys Val 
                1205                1210                1215    
Ala Lys Val Ile Glu Arg Gln Lys Asn Val Lys Ile Gly Tyr Thr Glu 
            1220                1225                1230        
Glu Leu Leu Met Lys Tyr Glu Thr Phe Leu Asp Lys Leu Lys Asn Tyr 
        1235                1240                1245            
Gly Ile Arg Leu Ser Thr Gln Ala Lys Thr Leu Glu Asn Gly Lys Glu 
    1250                1255                1260                
Lys Phe Glu Lys Leu Ser Leu Glu Asp Lys Cys Gln Val Leu Ser Glu 
1265                1270                1275                1280
Ile Leu His Leu Phe Gln Cys Gln Ser Ser Ala Ala Asn Asn Ala Leu 
                1285                1290                1295    
Ser Met Ile Gly Gly Pro Gly Asn Ile Leu Val Asn Asn Asn Ile Ser 
            1300                1305                1310        
Lys Cys Asn Lys Ile Ser Ile Ile Asn Gln Ser Pro Thr Gly Ile Tyr 
        1315                1320                1325            
Glu Gln Glu Ile Asp Leu Lys Lys Ile Gly Glu Asp 
    1330                1335                1340

<210> 18
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #69

<400> 18
Met Glu Lys Glu Tyr Tyr Val Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Ser Tyr His Ile Leu Arg Arg His Met 
            20                  25                  30          
Lys Ile Leu Gly Asn Thr Glu Gly Lys Ala His Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Glu Ser Ala Lys Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ser Arg Arg Arg Leu Asp Arg Arg Asn Trp Arg Ile Glu 
65                  70                  75                  80  
Ile Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Lys Val Asp Pro Gly 
                85                  90                  95      
Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro Glu Asp Lys Pro 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Lys Asp Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Glu 
    130                 135                 140                 
Thr Glu Glu Thr Pro Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Met Met Lys His Arg Gly His Phe Leu Leu Ser Gly Asp Ile Ser Glu 
                165                 170                 175     
Glu Val Thr Glu Phe Gly Thr Thr Phe Ser Gln Phe Leu Glu Asn Ile 
            180                 185                 190         
Lys Asn Glu Leu Asp Phe Asn Leu Leu Asp Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Leu Thr Arg Ser Ala Lys Lys Thr Lys 
    210                 215                 220                 
Leu Val Lys Gly Leu Lys Ala Glu Lys Lys Asn Val Cys Glu Lys Ala 
225                 230                 235                 240 
Leu Leu Thr Leu Leu Ser Gly Gly Thr Val Lys Leu Ser Asp Ile Phe 
                245                 250                 255     
Gly Met Gly Glu Arg Pro Lys Ile Ser Phe Ala Asp Asn Gly Tyr Glu 
            260                 265                 270         
Asp Tyr Ala Ala Val Val Glu Asn Glu Leu Gly Glu Gln Phe Tyr Glu 
        275                 280                 285             
Ile Ile Glu Ala Ala Lys Ala Val Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Ser Val Ser Glu Ala Lys Val Arg Thr Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Lys His Lys Ser Asp Leu Lys Tyr Leu Lys 
                325                 330                 335     
Ala Ile Val Lys Lys Tyr Leu Pro Glu Glu Tyr Lys Asp Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Leu Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Lys Glu Glu Phe Tyr Asp Phe Leu Lys Lys Asn Ile Lys Glu Leu Glu 
    370                 375                 380                 
Ala Gln Glu Tyr Leu Gln Glu Glu Leu Glu Lys Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Asn Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Tyr Glu Leu Lys Lys Ile Leu Asp Asn Leu Gly Asp Lys Ile Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Leu Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Lys Gly Asn Gly Glu Phe Ala Trp 
    450                 455                 460                 
Ala Val Arg Lys Ser Asn Glu Lys Ile Tyr Pro Trp Asn Phe Glu Asn 
465                 470                 475                 480 
Val Ile Asp Val Glu Ala Ser Ala Glu Lys Phe Ile Arg Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro Lys Asp Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Leu Asp Gly Glu Lys Ile Lys Lys Arg Lys Phe Ser Val Glu Leu Lys 
    530                 535                 540                 
Gln Lys Ile Tyr Arg Asp Val Phe Cys Arg Tyr Arg Lys Val Thr Ala 
545                 550                 555                 560 
Lys Lys Leu Lys Asn Tyr Leu Ile Arg Glu Gly Ile Thr Gly Lys Asn 
                565                 570                 575     
Val Glu Ile Thr Gly Ile Asp Gly Asp Phe Lys Gly Ser Leu Thr Ala 
            580                 585                 590         
Tyr His Asp Phe Lys Glu Ile Leu Gly Lys Gly Lys Leu Leu Asp Val 
        595                 600                 605             
Glu Leu Thr Gln Asn Glu Lys Glu Glu Ile Ile Leu Asn Ile Thr Leu 
    610                 615                 620                 
Phe Asp Asp Lys Lys Leu Leu Lys Lys Arg Leu Lys Lys Tyr Pro Gln 
625                 630                 635                 640 
Ile Leu Thr Glu Lys Gln Ile Lys Lys Ile Cys Ala Leu Ser Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Lys Lys Phe Leu Glu Glu Val Thr Ala Pro 
            660                 665                 670         
Glu Thr Gly Glu Asn Ile Ile Thr Ala Leu Trp Glu Thr Asp Pro Ser 
        675                 680                 685             
Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Glu Asp Tyr Arg Phe Met 
    690                 695                 700                 
Glu Glu Ile Glu Gln Phe Asn Ala Glu Lys Ala Asp Lys Thr Ser Tyr 
705                 710                 715                 720 
Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Gln Ile 
                725                 730                 735     
Trp Gln Thr Leu Gln Ile Val Lys Glu Ile Glu Lys Val Met Gly Glu 
            740                 745                 750         
Lys Pro Lys Arg Val Phe Val Glu Met Ala Arg Glu Lys Gln Glu Asn 
        755                 760                 765             
Lys Arg Gly Arg Glu Ser Arg Lys Lys Lys Leu Ser Asp Leu Tyr Lys 
    770                 775                 780                 
Ser Cys Lys Asn Glu Glu Pro Asp Trp Val Lys Glu Leu Gly His Glu 
785                 790                 795                 800 
Glu His Lys Leu Arg Ser Asp Lys Leu Tyr Leu Tyr Tyr Thr Gln Lys 
                805                 810                 815     
Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp Leu Trp 
            820                 825                 830         
Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Thr Met Asp Asp 
        835                 840                 845             
Ser Leu Asn Asn Arg Val Leu Val Lys Lys Glu Tyr Asn Ala Lys Lys 
    850                 855                 860                 
Ser Asp Thr Tyr Pro Ser Ala Asp Ile Arg Gln Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Lys Ser Leu Leu Thr Gly Gly Phe Ile Ser Lys Glu Lys Tyr Asp 
                885                 890                 895     
Arg Leu Ile Arg Asn Asn Arg Gly Glu Leu Ser Ala Asn Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Glu Arg Gln Ile Val Glu Thr Arg Gln Ser Thr Lys Ala 
        915                 920                 925             
Val Ala Glu Ile Leu Lys Gln Val Phe Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Lys Glu Thr Glu Ile Val Tyr Val Lys Ala Lys Thr 
945                 950                 955                 960 
Val Ser Lys Phe Arg Gln Asp Phe Asp Phe Ile Lys Val Arg Asp Val 
                965                 970                 975     
Asn Asp Leu His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val Gly 
            980                 985                 990         
Asn Thr Tyr Tyr Val Lys Phe Thr Lys Asn Ala Ala Trp Phe Ile Lys 
        995                 1000                1005            
Glu Asn Pro Gly Arg Thr Tyr Ala Arg Glu Glu Lys Lys Ala Asn Ala 
    1010                1015                1020                
Lys Leu His Phe Tyr Ser Asn Leu Lys Lys Met Phe Thr Ser Asp Val 
1025                1030                1035                1040
Ala Ile Glu Asp Arg Asn Gly Glu Ile Ala Trp Gly Lys Ser Gly Thr 
                1045                1050                1055    
Ile Val Thr Val Arg Lys Met Met Asn Lys Asn Asn Ile Leu Val Thr 
            1060                1065                1070        
Arg Arg Ser Tyr Glu Val Lys Gly Gly Leu Phe Asp Gln Gln Ile Met 
        1075                1080                1085            
Lys Lys Gly Lys Gly Gln Val Tyr Val Pro Val Lys Glu Arg Leu Ser 
    1090                1095                1100                
Ile Glu Lys Tyr Gly Gly Tyr Asn Lys Ala Ala Gly Ala Tyr Phe Met 
1105                1110                1115                1120
Leu Val Glu Ser Asp Ile Glu Glu Gly Lys Lys Gly Lys Glu Lys Arg 
                1125                1130                1135    
Thr Ile Glu Phe Val Pro Ile Pro Leu Tyr Asp Lys Ala Lys Leu Glu 
            1140                1145                1150        
Ser Asp Glu Ala Arg Phe Leu Glu Glu Arg Gly Leu Lys Glu Pro Lys 
        1155                1160                1165            
Val Asp Ile Leu Ile Lys Lys Ile Lys Asp Thr Leu Phe Glu Val Asp 
    1170                1175                1180                
Gly Phe Lys Met Trp Leu Ser Gly Arg Thr Leu Ile Phe Lys Cys Ala 
1185                1190                1195                1200
Asn Gln Leu Ile Leu Asp Glu Glu Asn Met Lys Thr Leu Lys Lys Val 
                1205                1210                1215    
Val Lys Phe Ile Gln Arg Gln Lys Asn Val Lys Ile Gly Ile Thr Glu 
            1220                1225                1230        
Glu Val Leu Met Lys Tyr Glu Thr Phe Leu Asp Lys Leu Lys Asn Tyr 
        1235                1240                1245            
Gly Ile Arg Leu Ser Ser Gln Ala Lys Thr Leu Val Asn Gly Lys Glu 
    1250                1255                1260                
Lys Phe Glu Lys Leu Ser Leu Glu Asp Lys Cys Gln Val Leu Tyr Glu 
1265                1270                1275                1280
Ile Leu His Leu Phe Gln Cys Gln Ser Ser Ala Ala Asn Asn Ala Leu 
                1285                1290                1295    
Lys Met Ile Gly Gly Pro Gly Asn Ile Leu Val Asn Asn Asn Ile Ser 
            1300                1305                1310        
Lys Cys Asn Lys Ile Ser Ile Ile Asn Gln Ser Pro Thr Gly Ile Tyr 
        1315                1320                1325            
Glu Lys Glu Ile Asp Leu Leu Lys Ile Gly Glu Asp 
    1330                1335                1340

<210> 19
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #71

<400> 19
Met Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Ser Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Asp Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Leu Phe Lys Gln Phe Val Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Leu Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Arg Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Ile Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Ala Met Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Thr Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Asp Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Arg Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Val Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ala Glu Glu Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Asn Tyr Ile Asp Ser 
                565                 570                 575     
Pro Thr Ile Lys Gly Ile Glu Asp Glu Phe Asn Ala Ser Phe Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Gly Val Ser Arg Glu Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ser Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ala Ile Asn Glu Thr Glu Asn Leu Gln 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Gln Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu Asp Ser Arg Phe Asn Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Asn Asn Ala His Glu Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Ile Met Lys Phe Phe Ala Ser Glu Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Ile Ala Thr Val Lys Lys Val Leu Ser Tyr His Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Tyr Leu Glu Lys Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asp Val Leu Met Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ser His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Ile Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Glu 
            1220                1225                1230        
Glu His Arg Gln Glu Phe Asp Glu Leu Leu Asp Tyr Ile Ile Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Gln Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Arg Leu Gly Glu Leu Gly Glu Asp 
    1330                1335                1340

<210> 20
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #72

<400> 20
Met Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Leu Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Lys Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Ala Met Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Asp Lys Tyr Tyr Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Asp Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Lys Gln Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Asp Ser 
                565                 570                 575     
Pro Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Gly Val Ser Arg Glu Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ser Ile Asn Glu Thr Glu Asn Leu Gln 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Gln Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Ser Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Ile Met Lys Phe Phe Ala Ser Glu Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Ile Ala Thr Val Lys Lys Val Leu Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Tyr Leu Glu Lys Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asp Val Leu Met Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ser His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Tyr Ile Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Gln Glu Phe Asp Glu Leu Leu Asp Tyr Val Ile Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Gln Asn Lys Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Arg Leu Gly Glu Leu Gly Glu Asp 
    1330                1335                1340

<210> 21
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #73

<400> 21
Met Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Pro Met Ser Glu Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Thr Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Ile Ser Glu Thr Phe Glu Gln Phe Leu Asp Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Leu Val Asp Glu Thr Ile Lys Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Lys Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ile Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Gly Met Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Ala His Leu Pro Asp Lys Tyr Tyr Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Ser Lys Thr Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Asp Arg Gln Ser Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Val Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Lys Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Ile Glu Ile Phe Asp Asp Leu Phe Lys Gln Lys Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Val Asn Tyr Leu Glu Asn Glu Tyr Gln Tyr Ile Asp Ser 
                565                 570                 575     
Pro Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Gly Val Ser Lys Glu Phe Leu Asp Asp 
        595                 600                 605             
Pro Glu Asn Glu Glu Ile Leu Glu Asp Ile Ile Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ser Ile Asn Glu Thr Glu Asn Leu Gln 
705                 710                 715                 720 
Glu Ile Val Asn Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Gln Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Asp Ser Asn Leu Leu Lys Glu Lys Pro Leu Asp 
785                 790                 795                 800 
Asn Glu Gln Leu Lys Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asn Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Leu Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Asn Val Asn Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Lys Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Asn Leu Lys Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Ser Asn Met Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys His Phe Tyr Ser Asn Ile Met Lys Phe Phe Ser Ser Glu Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Ile Ser Thr Val Lys Lys Val Leu Ser Tyr Arg Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Lys Gln Lys Gly Arg Phe Tyr Lys Glu Thr Ile His 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Ile Lys Asn Asn Leu Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Glu Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Pro Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Thr Ala Tyr Leu Glu Lys Thr Gly Tyr Asn Asn Pro Lys 
        1155                1160                1165            
Glu Asp Val Leu Met Glu Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ser His Leu Val Glu Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Tyr Ile Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Gln Glu Phe Asp Glu Leu Leu Asp Tyr Val Ile Asp Phe 
        1235                1240                1245            
Ser Asn Lys Tyr Ile Ser Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Lys Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Thr Ile Ser Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asp Ser Thr Leu Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Arg Leu Glu Glu Leu Gly Glu Asp 
    1330                1335                1340

<210> 22
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #74

<400> 22
Met Lys Lys Asn Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Lys Asp Asp Tyr Thr Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Gln Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Pro Met Ser Glu Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Asp Thr Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Ile Ser Glu Thr Phe Glu Gln Phe Leu Asp Thr Tyr 
            180                 185                 190         
Asn Glu Ile Phe Ser Glu Gln Glu Leu Val Asp Glu Ile Thr Lys Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Gln 
    210                 215                 220                 
Val Leu Ala Leu Phe Pro Asn Glu Lys Arg Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Asp Asp Ile Lys Leu Gln Leu Ser Glu Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Gly Met Ile Asp Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Asp Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Gln 
305                 310                 315                 320 
Met Ile Glu Leu Tyr Asp Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Ala His Leu Pro Asp Lys Tyr Tyr Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Val Leu Lys Lys Thr Asp 
    370                 375                 380                 
Asn Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Ser Phe Tyr Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Leu Arg Ala Ile Leu Asp Arg Gln Ser Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Arg Asp Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Glu Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Ile Val Asp Leu Glu Lys Ser Ala Glu Lys Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys Arg Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Glu Arg Gly Lys Thr Gln Asn Phe Ser Ser Gln Glu Lys 
    530                 535                 540                 
Ile Glu Ile Phe Asp Asp Leu Phe Lys Gln Lys Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Val Asn Tyr Leu Glu Asn Glu Tyr Gln Tyr Ile Asp Ser 
                565                 570                 575     
Pro Asp Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Ala Lys Ile Gly Val Ser Lys Glu Tyr Leu Asp Asp 
        595                 600                 605             
Pro Glu Asn Glu Glu Ile Leu Glu Asp Ile Ile Lys Ile Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Arg Lys Lys Gln Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 
Thr Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Lys His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp Glu 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Ser Phe Lys 
    690                 695                 700                 
Glu Lys Ile Glu Lys Ala Gln Thr Ile Asn Glu Thr Asn Asn Ile Gln 
705                 710                 715                 720 
Glu Ile Val Asn Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Ile Glu Ile Met Gly Tyr 
            740                 745                 750         
Gln Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Lys Lys Gly Lys Asn Asn Ser Lys Ala Arg Leu Lys Lys Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Asp Ser Asn His Val Lys Glu Gln Pro Leu Asp 
785                 790                 795                 800 
Asn Glu Gln Leu Lys Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asn Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Lys Asp Ile Val Arg Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Lys Gln Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Arg Ala 
            900                 905                 910         
His Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Leu Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Asn Ile Asn Ile Val Leu Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Lys Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Asn Leu Lys Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Asn Ser Met Phe Lys Glu Asp Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys His Phe Tyr Ser Asn Ile Met Lys Phe Phe Ser Lys Glu Lys 
1025                1030                1035                1040
Leu Val Asn Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Leu Ser Thr Ile Lys Lys Val Leu Ser Tyr Lys Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Lys Gln Lys Gly Arg Leu Phe Lys Glu Thr Ile His 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Ile Lys Asn Asn Leu Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Ser Glu Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Pro Ile Met Asp Arg Lys Ala Phe Glu 
            1140                1145                1150        
Lys His Pro Thr Ala Tyr Leu Glu Lys Thr Gly Tyr Asn Asn Pro Thr 
        1155                1160                1165            
Glu Asp Val Leu His Glu Leu Ser Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Ser Arg Arg Met Ile Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ser His Leu Val Glu Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Tyr Asp Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ala Lys Phe Asp Glu Leu Leu Asp Tyr Val Ile Asp Phe 
        1235                1240                1245            
Ser Asn Lys Tyr Ile Asn Ala Asp Lys Asn Ile Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Lys Glu Asn Lys Glu Ala Asp Val Lys Glu Leu Val Glu Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Thr Ile Ser Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Gly Ser Thr Leu Ile Phe Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Arg Leu Glu Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 23
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #75

<400> 23
Met Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Lys Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Tyr Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Asp Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Lys Gln Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Glu Ser 
                565                 570                 575     
Pro Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Ile Gly Val Ser Arg Glu Val Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Leu Asp Glu Glu Ile Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ser Ile Thr Glu Thr Glu Asn Leu Gln 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Gln Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Lys Glu Val Val Lys Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Lys Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Ser Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Ile Met Lys Phe Phe Ala Ser Glu Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Asp 
                1045                1050                1055    
Ile Ala Thr Ile Lys Lys Val Leu Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Tyr Leu Glu Lys Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asp Val Leu Met Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ser His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Tyr Ile Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Gln Glu Phe Asp Glu Leu Leu Asp His Val Ile Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Gln Asn Lys Glu Ala Asp Ile Lys Glu Leu Ala Gln Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Thr Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Arg Leu Gly Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 24
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #76

<400> 24
Met Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Arg Met 
            20                  25                  30          
Lys Ile His Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Asp Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asn Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Asn Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Glu Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Asp Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ser Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Tyr Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Lys Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Tyr Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Asp Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Asn Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Glu Cys Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asn Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Glu Ser 
                565                 570                 575     
Pro Thr Ile Lys Gly Ile Glu Asp Asn Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Ile Lys Ile Gly Val Ser Arg Glu Val Leu Asp Asp 
        595                 600                 605             
Glu Asp Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Leu Asp Glu Glu Ile Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Leu Thr Thr Glu Thr Glu Asn Leu Lys 
705                 710                 715                 720 
Asp Ile Val Asn Asn Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Gln Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Gly Ser Asp Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Lys Glu Val Val Lys Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Tyr Lys Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Asp Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Ser Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Ile Met Glu Phe Phe Ser Ser Glu Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Asp 
                1045                1050                1055    
Ile Ala Thr Ile Lys Lys Val Leu Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Thr Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Ile 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Tyr Leu Glu Lys Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asp Val Leu Met Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ser His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Tyr Ile Glu Ser Asn Asn Ala Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Gln Gln Phe Asp Glu Leu Leu Asp His Val Ile Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Lys Glu Ala Asp Ile Lys Glu Leu Ala Gln Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Gln Phe Asn Lys Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Lys Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 25
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #77

<400> 25
Met Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Arg Met 
            20                  25                  30          
Lys Ile Asn Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Lys Asp Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asn Glu Ser Phe Tyr Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Asn Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Glu Glu Thr Phe Lys Glu Phe Leu Gln Glu Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Leu Gln Pro Pro Val Asp Glu Asp Ile Asp Val 
        195                 200                 205             
Glu Ala Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Asn Leu Phe Pro Thr Glu Lys Ser Asn Gly Met Phe His Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ser Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Tyr Glu Ile Phe Arg 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Glu Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Ile Lys Lys Ile Asp Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Tyr Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Glu Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Lys Phe Ala Trp 
    450                 455                 460                 
Ile Glu Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Ser Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Glu Cys Asn Phe Ser Ser Lys Glu Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asn Leu Phe Lys Lys Asn Arg Lys Val Ser Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Glu Ser 
                565                 570                 575     
Pro Thr Ile Lys Gly Ile Glu Asn Asn Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Ile Lys Ile Gly Val Ser Arg Glu Val Leu Asp Asn 
        595                 600                 605             
Glu Asp Tyr Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Leu Asp Glu Glu Ile Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Thr Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ala Leu 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Lys Glu Ile Glu Lys Ala Gln Leu Lys Thr Glu Ser Glu Asn Leu Lys 
705                 710                 715                 720 
Asp Ile Val Asp Asn Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Gln Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Ile Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Gly Ser Asp Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Ser Lys Leu Tyr Lys Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Ser Ile Leu His Gln Arg Phe Asn Thr Glu Asn Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Asp Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Ser Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Leu Met Glu Phe Phe Ser Ser Glu Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Asp 
                1045                1050                1055    
Ile Ala Thr Ile Lys Lys Val Leu Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Thr Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Glu Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Ile 
1105                1110                1115                1120
Val Ile Thr His Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ile Phe Glu 
            1140                1145                1150        
Lys Asn Gln Ile Ala Tyr Leu Glu Asn Lys Gly Tyr Val Asn Pro Lys 
        1155                1160                1165            
Glu Asp Val Leu Met Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Asn Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ser His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Tyr Ile Glu Ser Asn Asn Ala Glu Ser Glu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg His Gln Phe Asp Glu Leu Leu Asp His Val Ile Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Thr Leu Ala Asp Thr Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Lys Glu Ala Glu Ile Lys Glu Leu Ala Gln Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Thr Phe Asn Lys Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Asn Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Lys Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 26
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #78

<400> 26
Met Lys Lys Gln Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Ser Asp Asp Tyr Asp Leu Ile Arg Lys Arg Met 
            20                  25                  30          
Lys Ile Asn Gly Asp His Glu Lys Gly Ser Met Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Ile 
    50                  55                  60                  
Arg Arg Thr Thr Arg Arg Arg Leu Arg Arg Arg Arg Asn Arg Val His 
65                  70                  75                  80  
Tyr Leu Gln Ser Ile Phe Lys Asp Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asn Glu Ser Phe Tyr Val Pro Met Asp Lys Glu 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 
Ser Asn Glu Gln Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Glu Glu Thr Phe Lys Asp Phe Leu His His Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Leu Gln Pro Pro Ile Ser Glu Asp Ile Ser Val 
        195                 200                 205             
Glu Ala Asp Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Asn Lys Phe Gly Thr Glu Lys Ser Asn Gly Met Phe His Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Ser Leu Asp Glu Asp Arg Lys Leu Asn Ile Thr Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Glu Ile Gly Glu Asp Tyr Arg Glu 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Val His Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Val Ala Asp Asp Lys Thr Lys Ala Ser Leu Ser Ser Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Gln Asp Leu Ser Ala Leu Lys 
                325                 330                 335     
Asn Phe Ile Lys Ala His Leu Pro Asp Arg Tyr Tyr Asp Ile Phe Arg 
            340                 345                 350         
Asn Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Glu Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Val Leu Asp Lys Val Asp 
    370                 375                 380                 
Gly Ala Glu Ser Phe Ile Lys Lys Ile Asp Glu Glu Arg Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Tyr Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Glu Lys Gln Gly Gln Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Gln Ser Lys Phe Ala Trp 
    450                 455                 460                 
Ile Glu Arg Lys Gly Asp Glu Ser Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Glu Val Asp Leu Val Ser Ser Ala Glu Lys Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Ile Gln Lys Phe Asn Val Phe Asn Glu Leu Thr Lys Val Gln 
        515                 520                 525             
Tyr Val Asp Asp Gln Gly Lys Arg Cys Asn Phe Ser Ser Lys Glu Lys 
    530                 535                 540                 
Gln Glu Ile Tyr Asp Asn Leu Phe Lys Lys Asn Arg Thr Val Ser Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asp Tyr Leu Lys Asn Glu Tyr Gln Tyr Leu Glu Asn 
                565                 570                 575     
Pro Thr Ile Lys Gly Ile Glu Asn Lys Phe Asn Ala Ser Leu Lys Thr 
            580                 585                 590         
Tyr His Asp Phe Ile Lys Leu Gly Val Ser Arg Asp Val Leu Asp Asn 
        595                 600                 605             
Glu Asn Tyr Glu Glu Arg Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Phe Ser Asp 
625                 630                 635                 640 
Val Leu Asp Gln Asp Ile Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Gln Lys Leu Ile Asp Gly Ile Arg Asp Glu 
            660                 665                 670         
His Thr Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ala Pro 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asp Leu Ser Phe Lys 
    690                 695                 700                 
Lys Thr Ile Glu Glu Ala Arg Leu Lys Thr Glu Ser Asp Asn Leu Lys 
705                 710                 715                 720 
Asp Ile Val Asp Lys Ile Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Glu Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Gln Gly Gln Arg Asn Ala Lys Glu Arg Leu Lys Arg Ile Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Lys Ser Asp Leu Leu Lys Lys His Pro Val Asp 
785                 790                 795                 800 
Gln Glu Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Asn Gln Glu Leu Asp Ile Asn Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Thr Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Asp Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Glu Glu Val Val Lys Asn Met Lys Pro Phe 
865                 870                 875                 880 
Trp Ser Ser Leu Tyr Arg Ser Asn Leu Ile Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Ser Ile Leu His Gln Arg Tyr Asn Thr Glu Asn Asp Glu Glu 
    930                 935                 940                 
Ala His Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Ala Phe Asp Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Leu Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Asp Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Thr Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Leu Ile Glu Arg Phe Ser His Glu Asp 
1025                1030                1035                1040
Gln Ile Ile Asp Glu Asn Gly Glu Ile Val Trp Asp Lys Ser Lys Asp 
                1045                1050                1055    
Ile Ala Lys Ile Lys Arg Val Leu Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Thr Gly Asn Phe Ser Lys Glu Thr Val Gln 
        1075                1080                1085            
Pro Lys Gly Glu Ser Asn Lys Leu Ile Pro Arg Lys Lys Asp Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Ile 
1105                1110                1115                1120
Ile Phe Thr His Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Glu 
                1125                1130                1135    
Val Lys Glu Ile Ile Gly Ile Thr Ile Met Asp Gln Ser Ile Phe Glu 
            1140                1145                1150        
Ala Asp Gln Ile Lys Tyr Leu Glu Asn Lys Gly Tyr Val Asn Pro Lys 
        1155                1160                1165            
Glu Asp Ile Lys Phe Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Asn Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ser His Leu Val Thr Leu Leu Tyr His Cys 
                1205                1210                1215    
Lys Arg Tyr Ile Asp Ser Ser Asn Ala Asp His Glu Thr Tyr Ile Asn 
            1220                1225                1230        
Glu Asn Arg His Gln Phe Asn Glu Leu Leu Asp His Val Leu Asn Phe 
        1235                1240                1245            
Ser Ala Gln Tyr Thr Leu Ala Glu Ala Asn Glu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asn Gln Ala Glu Val Ser Glu Leu Ala Thr Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Asp Phe Asn Lys Met Gly Ala Pro Ser Ala Phe 
                1285                1290                1295    
Lys Phe Phe Gly Thr Asn Ile Glu Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Lys Glu Val Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 27
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #79

<400> 27
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Asp Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Thr Lys Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu Asn Leu Pro Glu Lys Tyr His Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Asp Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Lys Gln Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu Ser 
                565                 570                 575     
Ala Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp Asp 
        595                 600                 605             
Pro Glu Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Ser Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Leu Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ser Ile Thr Asp Thr Glu Asn Leu Gln 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Gln Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Arg Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr His Lys Phe Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Ile Met Lys Phe Phe Ala Ser Asp Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Asn 
                1045                1050                1055    
Ile Ala Thr Val Lys Lys Val Leu Asn Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Arg Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Asn Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asp Val Leu Met Lys Leu Pro Lys Tyr Thr Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Tyr Asp Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Gln Glu Phe Ser Glu Ile Leu Glu His Val Ile Asp Phe 
        1235                1240                1245            
Ala Glu Lys Tyr Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Gln Lys 
    1250                1255                1260                
Leu Tyr Glu Gln Asn Gln Glu Ala Asp Ile Lys Glu Leu Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Arg Leu Gly Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 28
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #80

<400> 28
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Arg Leu Val Ala Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Ala Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Glu Leu Gln Glu Ile Phe Ala Glu Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      
Phe Phe Ala Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Ser Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Lys Ser Tyr 
        115                 120                 125             
His Gln Thr Tyr Pro Thr Ile Tyr His Leu Arg Gln His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Leu Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Arg Gln Phe Leu Gln Thr Tyr 
            180                 185                 190         
Asn Gln Gln Phe Ser Glu Pro Glu Pro Val Asp Glu Ser Val Asp Val 
        195                 200                 205             
Ala Val Ile Leu Thr Glu Lys Leu Ser Lys Thr Lys Lys Ala Glu Lys 
    210                 215                 220                 
Val Leu Lys Tyr Phe Pro Asn Glu Lys Ser Asn Gly Tyr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Met Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Glu Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Glu Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Gln Ala Lys Asn Val Tyr Asp Ala Ile Leu Leu Ser Glu 
    290                 295                 300                 
Ile Leu Ser Thr Thr Thr Lys Asn Thr Lys Ala Lys Leu Ser Ala Gly 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu Asn Leu Pro Glu Lys Tyr His Glu Leu Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Glu Leu Ser Gly Ile Asp 
    370                 375                 380                 
Gly Ser Glu Pro Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Thr Glu Leu Arg Ala Ile Ile Asp Arg Gln Lys Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Ala Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Gln Ser Pro Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Glu Glu Lys Ile Lys Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Met Glu Lys Ser Ala Met Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Met Ile Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Lys Asp Glu Arg Gly Lys Thr His Tyr Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Arg Glu Ile Phe Asp Glu Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Gln Asn Phe Leu Tyr Leu Asn Tyr Asn Tyr Ile Glu Asn 
                565                 570                 575     
Ala Glu Ile Lys Gly Ile Glu Lys Ala Phe Asn Ala Ser Tyr Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Leu Ser Glu Met Glu Glu Trp Leu Glu Asp 
        595                 600                 605             
Pro Glu Met Glu Ser Met Phe Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Thr Gln Leu Ser Lys Tyr Glu Ala 
625                 630                 635                 640 
Val Leu Gly Glu Glu Ile Leu Lys Lys Leu Ala Lys Lys His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Gln Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly Phe 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ala Ile Thr Asp Thr Glu Asn Leu Gln 
705                 710                 715                 720 
Glu Ile Val Lys Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Leu Lys Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Asp Phe Gly Ser Gln Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Pro Leu Asp Ile His Arg Leu Ser 
            820                 825                 830         
Asp Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Lys Asp Asp Val Pro Ser Glu Lys Val Val Lys Lys Met Arg Pro Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Arg Ala Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ile Glu Arg Gly Gly Leu Thr Glu Glu Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Ser Lys Lys Glu Asp Glu 
    930                 935                 940                 
Gly Lys Ala Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Ile Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Ile Ala Leu Leu Lys Lys Tyr Pro Gln Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Leu Lys Phe Asn Ala His Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Glu Phe Tyr Ser Asn Ile Met Lys Phe Phe Glu Ser Asp Glu 
1025                1030                1035                1040
Pro Ile Cys Asp Glu Asn Gly Glu Ile Phe Trp Asp Lys Ser Lys Asn 
                1045                1050                1055    
Ile Ala Gln Ile Lys Lys Val Ile Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Val Gln 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Ala Lys Tyr Gly Gly Phe Gly Ser Pro Asn Val Ala Tyr Thr Val 
1105                1110                1115                1120
Ala Phe Thr Tyr Asp Ile Glu Lys Gly Lys Ala Arg Lys Leu Lys Arg 
                1125                1130                1135    
Ala Lys Ala Ile Glu Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Gln Asn Pro Ile Ala Phe Leu Lys Asn Lys Gly Tyr Glu Asn Ala Lys 
        1155                1160                1165            
Glu Asp Val Leu Met Lys Leu Pro Lys Tyr Thr Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Val Ala Ser His Lys Glu Ala Gln Lys Ala 
1185                1190                1195                1200
Asn Ser Phe Ile Leu Pro Glu His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Tyr Asp Glu Ile Ser His Lys Glu Ser Phe Asp Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ser Glu Phe Ser Glu Ile Leu Asp His Val Ile Asp Phe 
        1235                1240                1245            
Ala Gly Lys Tyr Thr Leu Ala Asp Lys Asn Ile Glu Lys Ile Gln Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Gln Glu Asp Asp Ile Ala Glu Leu Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Asp Val Thr Ile Pro Arg Lys Arg Tyr Thr Ser Leu Thr 
            1300                1305                1310        
Glu Ile Trp Asp Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Arg Met Gly Asn Leu Gly Glu Gln 
    1330                1335                1340

<210> 29
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #81

<400> 29
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Arg Leu Val Ala Lys Lys Met 
            20                  25                  30          
Ser Val His Gly Asn Thr Glu Lys Lys Lys Val Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Ala Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Glu Leu Gln Glu Ile Phe Ala Glu Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      
Phe Phe Ala Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Thr Arg Phe Pro Ile Phe Pro Thr Leu Glu Glu Glu Lys Ser Tyr 
        115                 120                 125             
His Gln Thr Tyr Pro Thr Ile Tyr His Leu Arg Gln His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Leu Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Glu Leu Asp Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Arg Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Gln Phe Ser Glu Pro Glu Pro Val Asp Glu Ser Glu Gly Val 
        195                 200                 205             
Ala Val Ile Leu Thr Glu Lys Leu Ser Lys Thr Lys Lys Val Glu Lys 
    210                 215                 220                 
Val Leu Lys Tyr Tyr Pro Asn Glu Lys Ser Asn Gly Tyr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Glu Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Glu Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Gln Ala Lys Asn Val Tyr Asp Ala Ile Leu Leu Ser Glu 
    290                 295                 300                 
Ile Leu Ser Thr Thr Lys Lys Asn Thr Lys Ala Lys Leu Ser Ala Gly 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Lys Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu Asn Leu Pro Glu Lys Tyr Ala Glu Phe Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Glu Leu Ser Gly Ile Asp 
    370                 375                 380                 
Gly Ser Glu Pro Phe Leu Glu Lys Ile Asp Gln Glu Asn Phe Leu Leu 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Ala Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Thr Glu Leu Arg Ala Ile Ile Asp Arg Gln Lys Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Ala Gln Glu Lys Ile Glu Gln Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Gln Gly Gln Ser Pro Phe Ala Trp 
    450                 455                 460                 
Leu Glu Arg Lys Ser Ala Glu Lys Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Met Glu Lys Ser Ala Met Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Gln Asp Thr Tyr Leu Pro Thr Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Met Ile Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Lys Asp Glu Arg Gly Lys Thr His Tyr Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Arg Glu Ile Phe Asp Glu Leu Phe Lys Lys His Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Gln Asn Phe Leu Tyr Leu Asn Tyr Asn Tyr Ile Glu Asn 
                565                 570                 575     
Ala Glu Ile Lys Gly Ile Glu Lys Ala Phe Asn Ala Ser Tyr Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Leu Ala Glu Met Glu Glu Trp Leu Glu Asp 
        595                 600                 605             
Pro Glu Met Glu Ser Met Phe Glu Asp Ile Val Lys Ile Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Thr Gln Leu Ser Lys Tyr Glu Ala 
625                 630                 635                 640 
Val Leu Gly Glu Gln Val Leu Lys Lys Leu Ala Lys Lys His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Glu Lys Leu Ile Gln Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Asp Phe 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp His Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Ala Lys Ala Leu Ala Ile Ser Asp Ser Glu Ser Leu Leu 
705                 710                 715                 720 
Glu Val Val Lys Ala Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Trp Gln Ser Leu Lys Ile Val Glu Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Asn Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Leu Lys Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Asn Phe Gly Ser Pro Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Pro Leu Asp Ile His Arg Leu Ser 
            820                 825                 830         
Glu Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Ile Val Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Met Asp Asp Val Pro Ser Glu Lys Val Val Lys Lys Met Arg Pro Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Gln Arg Ala Lys Leu Ile Ser Glu Arg Lys Phe Ala 
                885                 890                 895     
Asn Leu Thr Lys Ile Glu Arg Gly Gly Leu Thr Glu Glu Asp Lys Ala 
            900                 905                 910         
Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln His Phe Asn Gln Lys Lys Glu Asp Glu 
    930                 935                 940                 
Gly Lys Ala Ser Gly Lys Val Arg Ile Ile Thr Leu Lys Ser Thr Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Ile Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asn His His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Ile Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asn Tyr Ile Lys Phe Asn Leu His Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Glu Phe Tyr Ser Asn Ile Met Lys Phe Phe Glu Ser Glu Glu 
1025                1030                1035                1040
Pro Ser Cys Asp Glu Asn Gly Glu Ile Phe Trp Asp Lys Ser Arg Asn 
                1045                1050                1055    
Ile Ala Gln Ile Lys Lys Val Ile Ser Ser His Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Tyr Lys Glu Thr Val Asn 
        1075                1080                1085            
Pro Lys Gly Asn Pro Asp Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Ser Lys Tyr Gly Gly Phe Gly Ser Pro Val Val Ala Tyr Thr Val 
1105                1110                1115                1120
Ala Phe Ile Tyr Asp Ile Glu Lys Gly Lys Ala Arg Lys Leu Lys Arg 
                1125                1130                1135    
Ala Lys Ala Ile Glu Gly Ile Thr Ile Met Glu Gln Ser Ala Phe Glu 
            1140                1145                1150        
Gln Asp Pro Ile Ala Phe Leu Lys Asn Lys Gly Tyr Glu Asn Val Lys 
        1155                1160                1165            
Glu Asp Glu Phe Met Lys Leu Pro Lys Tyr Thr Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Val Ala Ser His Lys Glu Ala Gln Lys Ala 
1185                1190                1195                1200
Asn Ser Phe Ile Leu Pro Glu His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Gln His Tyr Asp Glu Ile Ser His Lys Glu Ser Phe Asp Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ser Glu Phe Ser Glu Ile Leu Asp His Val Ile Asp Phe 
        1235                1240                1245            
Ala Gly Arg Tyr Thr Leu Ala Asp Lys Asn Ile Glu Lys Ile Gln Lys 
    1250                1255                1260                
Leu Tyr Glu Gln Asn Gln Glu Asp Asp Ile Ala Glu Leu Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Asp Val Thr Ile Pro Arg Lys Arg Tyr Thr Ser Leu Thr 
            1300                1305                1310        
Glu Ile Trp Asp Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Arg Met Gly Asn Leu Gly Glu Gln 
    1330                1335                1340

<210> 30
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #82

<400> 30
Met Ala Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Arg Leu Met Ala Lys Lys Met 
            20                  25                  30          
Ser Val His Gly Asn Thr Glu Lys Lys Lys Val Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Ala Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Glu Leu Gln Glu Ile Phe Ala Glu Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      
Phe Phe Val Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Gln 
            100                 105                 110         
Tyr Thr Arg Phe Pro Ile Phe Pro Thr Leu Glu Glu Glu Lys Ser Tyr 
        115                 120                 125             
Tyr Gln Thr Tyr Pro Thr Ile Tyr His Leu Arg Gln Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Leu Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Glu Leu Asp Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Glu Ile Tyr 
            180                 185                 190         
Asn Gln Gln Phe Asp Gln Pro Leu Ala Val Asp Glu Ser Glu Gly Val 
        195                 200                 205             
Ala Val Ile Leu Thr Glu Lys Leu Ser Lys Thr Lys Lys Val Glu Lys 
    210                 215                 220                 
Ile Leu Lys Tyr Tyr Pro Thr Glu Lys Thr Asn Gly Cys Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Glu Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ser Leu Leu Glu Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Gln Ala Lys Lys Val Tyr Asp Ala Ile Leu Leu Ser Glu 
    290                 295                 300                 
Ile Leu Ser Thr Thr Lys Lys Asn Thr Lys Ala Lys Leu Ser Ala Gly 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Ser His Lys Lys Asp Leu Ala Lys Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu Asn Leu Pro Glu Lys Thr Ala Val Phe Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Glu Leu Ser Gly Ile Val 
    370                 375                 380                 
Gly Ser Glu Pro Phe Leu Glu Lys Ile Asp Gln Glu Asn Phe Leu Leu 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Ala Asn Gly Val Ile Pro His Gln Val His Leu 
                405                 410                 415     
Thr Glu Leu Lys Ala Ile Ile Asp Arg Gln Lys Gln Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Glu Glu Ala Arg Asp Lys Ile Ile Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Gln Gly Gln Ser Ser Phe Ala Trp 
    450                 455                 460                 
Leu Glu Arg Lys Thr Ala Glu Lys Ile Thr Pro Trp Asn Phe Thr Glu 
465                 470                 475                 480 
Val Val Asp Ile Glu Lys Ser Ala Met Arg Phe Ile Gln Arg Met Thr 
                485                 490                 495     
Asn Gln Asp Thr Tyr Leu Pro Thr Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Tyr Met Ile Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Lys Asp Glu Arg Gly Ile Lys His Tyr Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Arg Glu Ile Phe Lys Glu Leu Phe Gln Lys Gln Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Lys Leu Gln Asn Phe Leu Tyr Ala Asn Tyr Asn Tyr Ile Glu Asn 
                565                 570                 575     
Ala Glu Ile Leu Gly Ile Glu Lys Ala Phe Asn Ala Ser Tyr Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Leu Ala Lys Met Gln Glu Trp Leu Glu Gln 
        595                 600                 605             
Pro Glu Met Glu Ser Met Phe Glu Asp Ile Val Lys Ile Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Thr Gln Leu Ser Lys Tyr Gln Ala 
625                 630                 635                 640 
Val Leu Gly Glu Lys Leu Leu Lys Lys Leu Ala Lys Lys His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Glu Lys Leu Ile His Gly Ile Arg Asp Lys 
            660                 665                 670         
Lys Thr Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Asp Phe 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp His Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Ala Lys Ala Leu Ala Leu Ser Asp Asn Gln Ser Leu Leu 
705                 710                 715                 720 
Glu Val Val Gln Ala Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Trp Gln Ser Leu Lys Ile Val Glu Glu Leu Ile Glu Ile Ile Gly Tyr 
            740                 745                 750         
Asn Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Lys Thr 
        755                 760                 765             
Asn Arg Gly Leu Lys Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Asn Phe Gly Ser Pro Leu Leu Lys Glu Gln Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Pro Leu Asp Ile Asp Arg Leu Ser 
            820                 825                 830         
Glu Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Ile Val Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Met Asp Asp Val Pro Ser Glu Lys Val Val Lys Lys Met Arg Arg Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Gln Arg Ala Lys Leu Ile Ser Glu Arg Lys Phe Ala 
                885                 890                 895     
Tyr Leu Thr Lys Ile Glu Arg Gly Gly Leu Thr Pro Glu Asp Lys Ala 
            900                 905                 910         
Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln His Phe Asn Gln Pro Gln Glu Asp Glu 
    930                 935                 940                 
Gly Lys Gly Cys Gly Lys Val Arg Ile Ile Thr Leu Lys Ser Thr Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Ile Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asn His His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Ile Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asn Tyr Ile Lys Phe Asn Leu Ala Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Glu Phe Tyr Ser Asn Ile Leu Lys Phe Phe Glu Ser Glu Glu 
1025                1030                1035                1040
Pro Ser Cys Asp Glu Asn Gly Glu Ile Phe Trp Asp Lys Ser Arg His 
                1045                1050                1055    
Ile Pro Gln Ile Lys Lys Val Ile Ser Ser His Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Lys Gly Gly Phe Tyr Lys Glu Thr Val Asn 
        1075                1080                1085            
Pro Lys Gly Lys Pro Asp Lys Leu Ile Gln Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Val Ser Lys Tyr Gly Gly Phe Gly Ser Pro Val Val Ala Tyr Ala Val 
1105                1110                1115                1120
Ala Phe Ile Tyr Asp Ile Glu Lys Gly Lys Ala Arg Lys Leu Lys Lys 
                1125                1130                1135    
Ala Lys Ala Ile Glu Gly Ile Thr Ile Met Glu Gln Ser Ala Phe Glu 
            1140                1145                1150        
Gln Asp Pro Ile Ala Phe Leu Lys Asn Lys Gly Tyr Ser Asn Val Thr 
        1155                1160                1165            
Glu Asp Lys Phe Ile Lys Leu Pro Lys Tyr Thr Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Val Ala Ser His Lys Glu Ala Gln Lys Ala 
1185                1190                1195                1200
Asn Ser Phe Ile Leu Pro Glu Gln Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Gln His Tyr Asp Glu Ile Ser His Lys Glu Ser Phe Asp Tyr Val Asn 
            1220                1225                1230        
Asp His Leu Ser Glu Phe Ser Glu Ile Leu Asp Gln Val Ile Asp Phe 
        1235                1240                1245            
Ala Gly Arg Tyr Thr Leu Ala Asp Lys Asn Ile Glu Lys Ile Gln Glu 
    1250                1255                1260                
Leu Tyr Glu Gln Asn Gln Glu Gly Asp Thr Gln Asn Leu Ala Gln Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Asp Val Thr Ile Pro Arg Lys Arg Tyr Thr Ser Leu Thr 
            1300                1305                1310        
Glu Ile Trp Asp Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Arg Met Gly Asn Leu Gly Glu Gln 
    1330                1335                1340

<210> 31
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #83

<400> 31
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val Tyr Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Thr Lys Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Val Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Glu Asn Leu Pro Glu Lys Tyr His Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile His Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Gln Gln Ile Phe Asp Asp Leu Phe Lys Gln Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu Ser 
                565                 570                 575     
Ala Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp Asp 
        595                 600                 605             
Pro Glu Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Leu Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ser Thr Thr Asn Thr Glu Asp Leu Gln 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Arg Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Glu 
    770                 775                 780                 
Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys Ala Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr His Lys Phe Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Ile Met Lys Phe Phe Ala Ser Asp Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Asn 
                1045                1050                1055    
Ile Ala Thr Val Lys Lys Val Leu Asn Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Arg Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Glu Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asp Val Leu Met Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Cys Asp Glu Ser Asn Asn Gly Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ala Glu Phe Ser Glu Ile Leu Glu His Val Ile Asp Phe 
        1235                1240                1245            
Ala Glu Lys Tyr Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Leu Lys 
    1250                1255                1260                
Leu Tyr Glu Gln Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Arg Leu Gly Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 32
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #84

<400> 32
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Glu Asp Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val Tyr Gly Asn Thr Glu Lys Lys Tyr Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Thr Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Ile 
        195                 200                 205             
Glu Glu Ile Phe Thr Glu Lys Val Ser Arg Ala Lys Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Thr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Thr Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Glu Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Ala Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Lys Asn Leu Pro Glu Lys Tyr His Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Ser Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile His Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Leu Ala Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Met Leu Tyr Glu Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Gln Gln Ile Phe Asp Asp Leu Phe Lys Gln Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu Ser 
                565                 570                 575     
Ala Thr Ile Lys Gly Ile Glu Asn Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Ile Gly Ile Thr Gln Glu Val Leu Asp Asp 
        595                 600                 605             
Pro Glu Asn Glu Glu Met Phe Glu Glu Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Leu Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ser Glu Thr Asn Thr Glu Asp Leu His 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Arg Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Glu 
    770                 775                 780                 
Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys Ala Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Arg Ser Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr His Lys Phe Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Ile Met Lys Phe Phe Ala Ser Asp Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Asn 
                1045                1050                1055    
Ile Ala Thr Val Lys Lys Val Met Asn Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Ser Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Arg Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Glu Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asp Val Leu Met Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Cys Asp Glu Ser Asn Asn Gly Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ala Glu Phe Ser Glu Ile Leu Glu His Val Lys Asp Phe 
        1235                1240                1245            
Ala Glu Lys Tyr Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Leu Lys 
    1250                1255                1260                
Leu Tyr Glu Gln Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Arg Leu Gly Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 33
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #85

<400> 33
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Glu Asn Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val Tyr Gly Asn Thr Glu Lys Lys Tyr Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Glu Lys Arg 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Glu Glu Thr Phe Lys Thr Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Ile 
        195                 200                 205             
Glu Glu Ile Phe Thr Glu Lys Val Ser Arg Ala Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Thr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Thr Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Glu Tyr Glu 
            260                 265                 270         
Glu Glu Leu Glu Ala Leu Leu Ala Ile Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Ala Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Lys Glu Leu Pro Glu Lys Tyr His Glu Ile Phe Arg 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Ser Val Ser 
        355                 360                 365             
Gln Ala Glu Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Pro 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile His Lys Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asp Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Leu Ala Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Met Leu Tyr Glu Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Lys Gln Ile Phe Asp Glu Leu Phe Lys Gln Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Leu Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu Asp 
                565                 570                 575     
Ala Thr Ile Lys Gly Ile Glu Asn Ser Phe Asn Ala Ser Tyr Gly Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Ile Gly Ile Thr Gln Glu Met Leu Asp Asp 
        595                 600                 605             
Pro Glu Asn Glu Glu Met Phe Glu Glu Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Phe Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Ala Lys Ala Gln Ser Glu Thr Asn Thr Glu Asp Leu His 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Arg Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Glu 
    770                 775                 780                 
Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu Tyr Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Ile Ala Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Lys Arg Ser Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Leu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
His Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Thr Val Arg Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr His Lys Phe Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Ile Met Lys Phe Phe Ala Ser Asp Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Asn 
                1045                1050                1055    
Ile Ala Thr Val Lys Lys Val Met Asn Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Thr Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Ser Arg Lys Thr Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Arg Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Glu Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asp Val Leu Met Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Asn Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Cys Asp Glu Ser Asn Asn Gly Lys Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ala Glu Phe Ser Glu Ile Leu Glu Tyr Val Lys Asp Phe 
        1235                1240                1245            
Ala Glu Lys Tyr Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Leu Lys 
    1250                1255                1260                
Leu Tyr Glu Gln Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Arg Leu Gly Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 34
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #86

<400> 34
Met Asn Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Asp Glu Asn Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Ile Gln Gly Asn Gly Asp Lys Lys Lys Val Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Ala Gly Glu Thr Ala Glu Gly Thr Arg Met 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Gly Glu Met Asp Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Glu Lys Arg 
            100                 105                 110         
Asn Glu Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Lys Glu Thr Phe Lys Thr Phe Leu Gln Ala Tyr 
            180                 185                 190         
Asn Gln Ala Phe Thr Val Gln Glu Pro Val Asn Glu Thr Ile Asp Ile 
        195                 200                 205             
Gly Ser Leu Phe Gln Glu Arg Val Ser Arg Gln Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Thr Phe Met Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Thr Phe 
                245                 250                 255     
Gly Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Glu Tyr Glu 
            260                 265                 270         
Glu Glu Leu Glu Gly Leu Leu Ala Ile Val Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Ala Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Lys Asp Ile Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Leu Ile Lys Arg Tyr Glu Glu His Lys Thr Asp Leu Glu Gln Leu Lys 
                325                 330                 335     
Lys Phe Val Arg Lys Glu Leu Pro Glu Lys Tyr Tyr Glu Val Phe Arg 
            340                 345                 350         
Asp Ser Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Ser Val Ser 
        355                 360                 365             
Gln Ala Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Glu Lys Ile Pro 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Arg Lys Gln Ser Arg Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asp Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Thr Asp Gly Ser Ile Arg Pro Trp Asn Phe Thr Glu 
465                 470                 475                 480 
Val Val Asp Glu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Ser Tyr Leu Pro Gln Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Met Leu Tyr Glu Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Glu Thr Gln Asn Phe Ser Ser Val Glu Lys 
    530                 535                 540                 
Lys Glu Ile Phe Asp Glu Leu Phe Lys Lys Asn Arg Lys Val Thr Tyr 
545                 550                 555                 560 
Ala Leu Leu Glu Asn Phe Leu Lys Asn Gln Tyr Met Tyr Ile Glu Thr 
                565                 570                 575     
Ala Glu Ile Lys Gly Ile Glu Lys Ser Phe Asn Ala Ser Tyr Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Gly Ile Ser Gln Glu Met Leu Asp Asp 
        595                 600                 605             
Pro Glu Asn Glu Glu Met Phe Glu Glu Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Lys Glu Gln Leu Ser Lys Tyr Ala Asp 
625                 630                 635                 640 
Tyr Phe Glu Ala Gly Val Leu Lys Lys Leu Val Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Lys Lys Thr Ile Leu Asp Tyr Leu Met Arg Asp Asp Asp Leu 
        675                 680                 685             
Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Ser Ile Ala Gln Gln Gln Ser Glu Asp Gln Thr Glu Asp Leu His 
705                 710                 715                 720 
Glu Ile Val Lys Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Thr Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Arg Lys Lys Ser Lys Pro Arg Gln Lys Val Leu Glu Glu 
    770                 775                 780                 
Ala Met Lys Glu Phe Gly Ser Asn Leu Leu Lys Glu Tyr Pro Pro Asp 
785                 790                 795                 800 
Asn Ala Asn Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Lys Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Met Val Leu Val Ser Ser Thr Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Lys Glu Val Val Glu Lys Met Ile Gly Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Lys Arg Ser Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Leu Asn Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
His Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu His Gln Lys Tyr Asn Ser Glu Asp Glu Asp Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Thr Thr Arg Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Gln Ile Tyr Gln Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Arg Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Gln Lys Phe Asn Leu Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Asn Phe Tyr Ser Asn Leu Met Arg Phe Phe Ala Ser Asp Gln 
1025                1030                1035                1040
Pro Lys Val Asp Glu Asn Gly Glu Ile Leu Trp Asp Gln Ser Lys Asn 
                1045                1050                1055    
Ile Ala Met Val Lys Lys Val Met Asn Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Ile Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asp Ser Ala Lys Leu Ile Ala Arg Lys Thr Glu Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Ile Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Arg Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Arg Gln Phe Glu 
            1140                1145                1150        
Lys Asn Glu Val Glu Phe Leu Glu Glu Lys Gly Tyr Gln Asn Pro Arg 
        1155                1160                1165            
Glu Asp Val Leu Val Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Ile Ala Ser Ala Ser Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Asn His Leu Val Glu Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Cys Asp Asp Leu Asn Asp Gly Lys Ser Leu Ala Tyr Leu Asn 
            1220                1225                1230        
Ala His Arg Ala Glu Phe Asn Glu Leu Phe Glu Tyr Val Lys His Phe 
        1235                1240                1245            
Ala Glu Arg Tyr Thr Leu Ala Asp Lys Asn Leu Lys Lys Leu Ser Lys 
    1250                1255                1260                
Leu Tyr Glu Gln Asn Gln Glu Ala Asp Ile Lys Val Met Ala Gln Ser 
1265                1270                1275                1280
Phe Leu Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Asp Thr Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Ile Leu Thr Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr His Trp Lys Leu Gly Glu Leu Gly Glu Asp 
    1330                1335                1340

<210> 35
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #87

<400> 35
Met Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val Tyr Gly Asn Thr Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Ile Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ala Asn Gly Glu Pro Leu Asp Glu Ser Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Thr Lys Lys Ala Glu Lys 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Lys Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Ile Gln Phe Ala Lys Glu Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Val Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Ser 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Glu Asn Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Gln Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Asn Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Gln Ala Ile Ile His Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Leu Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Gly Glu Glu Lys 
    530                 535                 540                 
Gln Gln Ile Phe Asp Asp Leu Phe Lys Gln Lys Arg Lys Val Lys Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Gln Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu Ser 
                565                 570                 575     
Ala Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp Asp 
        595                 600                 605             
Pro Glu Asn Ala Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Phe Ser Asp 
625                 630                 635                 640 
Val Leu Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe Lys 
    690                 695                 700                 
Asn Glu Ile Glu Lys Ala Gln Ser Ser Thr Asn Asp Glu Asp Leu Gln 
705                 710                 715                 720 
Glu Ile Val Asn Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Lys 
    770                 775                 780                 
Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Arg Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys Ala Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr His Lys Phe Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Thr Asn Ile Met Lys Phe Phe Ala Lys Asp Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Tyr 
                1045                1050                1055    
Leu Ala Thr Ile Lys Lys Val Leu Asn Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Lys 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Arg Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Thr Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Glu Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asp Val Leu Met Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Cys Asp Glu Ser Asn Asn Gly Glu Ser Leu Ala Tyr Val Glu 
            1220                1225                1230        
Glu His Arg Ala Glu Phe Ser Glu Ile Leu Glu His Val Ile Asp Phe 
        1235                1240                1245            
Ala Glu Arg Tyr Thr Leu Ala Asp Ala Asn Leu Glu Lys Ile Asn Lys 
    1250                1255                1260                
Leu Phe Glu Gln Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Ser Phe 
                1285                1290                1295    
Lys Phe Phe Glu Lys Thr Ile Glu Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Arg Leu Gly Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 36
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #88

<400> 36
Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          
Lys Val Ala Gly Asn Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  
Asn Arg Thr Thr Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Ala Asn 
                85                  90                  95      
Phe Phe Tyr Arg Leu Glu Asp Ser Phe Tyr Val Glu Ser Glu Lys Arg 
            100                 105                 110         
His Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Lys Leu Val Asn 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Lys Leu Asp Thr 
                165                 170                 175     
Lys Asn Thr Ser Val Asp Glu Val Phe Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         
Asn Gln Val Phe Ala Ser Asp Ile Arg Leu Glu Glu Asn Lys Asp Val 
        195                 200                 205             
Ala Lys Ile Leu Ser Glu Lys Val Thr Arg Arg Glu Lys Leu Glu Lys 
    210                 215                 220                 
Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser Thr Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Ile Ser Leu Ile Val Gly Ser Lys Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Asp Glu Lys Thr Asp Ile Glu Cys Ala Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Glu 
        275                 280                 285             
Ile Phe Val Ala Ala Lys Asn Ala Tyr Asn Ala Val Val Leu Ser Ser 
    290                 295                 300                 
Ile Ile Thr Val Thr Asp Thr Glu Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Asp Lys His Glu Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Ala Phe Ile Lys Val His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Asn 
            340                 345                 350         
Asp Val Glu Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Lys 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Met Lys Lys Met Leu Glu Asn Ile Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Ile Asp Lys Ile Glu Glu Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile Pro His Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Glu Ala Ile Leu His Gln Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Tyr Glu Lys Ile Lys Ser Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly Gln Ser Glu Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Lys Val Asp Phe Gly Lys Ser Ala Ile Asp Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Gln Gly Lys Thr His Tyr Phe Ser Gly Gln Glu Lys 
    530                 535                 540                 
Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Lys Arg Lys Val Lys Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Gln Phe Leu Arg Asn Met Ser Asn His Ile Glu Ser 
                565                 570                 575     
Pro Thr Ile Glu Gly Val Glu Asp Ser Phe Asn Ser Ser Phe Ala Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Val Gly Val Thr Gln Glu Ile Leu Asp Asn 
        595                 600                 605             
Pro Leu Asn Ala Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu Gln Gln Phe Ser Asp 
625                 630                 635                 640 
Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Ile Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe Lys 
    690                 695                 700                 
Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Ala Asp Lys Asp Ile Gln 
705                 710                 715                 720 
Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Gly Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Gly Lys Gly Lys Asn Asn Ser Lys Pro Arg Phe Lys Ser Leu Glu Lys 
    770                 775                 780                 
Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Glu Leu Arg Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Ala Asn Arg Glu Lys 
    850                 855                 860                 
Gly Asp Asp Val Pro Ser Leu Glu Val Val Arg Lys Arg Lys Val Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Gln Ala Asn Leu Met Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Cys Glu Lys Asp Glu Asn 
    930                 935                 940                 
Gly Asn Thr Ile Glu Gln Val Arg Ile Val Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr Lys Val Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Asn Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr His Gln Phe Asp Trp Phe Lys Ala Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Thr Asn Ile Met Leu Phe Phe Ala Lys Lys Asp 
1025                1030                1035                1040
Arg Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Tyr 
                1045                1050                1055    
Leu Asp Thr Ile Lys Lys Val Leu Ser Tyr Arg Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Ile Gln Lys Gly Glu Phe Ser Lys Ala Thr Ile Lys 
        1075                1080                1085            
Pro Lys Gly Asn Ser Ser Lys Leu Ile Pro Arg Lys Ala Asn Trp Asp 
    1090                1095                1100                
Pro Met Lys Tyr Gly Gly Phe Asp Ser Pro Asn Met Ala Tyr Ala Val 
1105                1110                1115                1120
Val Ile Glu Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Ile Ile Lys 
                1125                1130                1135    
Glu Lys Glu Ile Ile Arg Ile Thr Ile Met Glu Arg Thr Ala Phe Glu 
            1140                1145                1150        
Lys Asp Gln Lys Ala Phe Leu Glu Glu Lys Gly Tyr Arg Asn Pro Lys 
        1155                1160                1165            
Glu Asp Val Leu Thr Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Cys Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Asn Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Asn His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Asn Cys Glu Ala Ser Asn Asp Gly Lys Ser Leu Ala Tyr Ile Glu 
            1220                1225                1230        
Ser His Arg Glu Met Phe Ala Glu Leu Leu Ala His Val Ser Glu Phe 
        1235                1240                1245            
Ala Lys Arg Tyr Thr Leu Ala Asp Ala Asn Leu Glu Lys Ile Asn Gln 
    1250                1255                1260                
Leu Phe Glu Gln Asn Lys Glu Gly Asp Ile Lys Val Ile Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Ala Phe Asn Ala Met Gly Ala Pro Ala Ser Phe 
                1285                1290                1295    
Lys Phe Phe Glu Thr Thr Ile Glu Arg Lys Arg Tyr Asn Asn Leu Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Ala Arg Lys Arg Leu Asp Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 37
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #89

<400> 37
Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          
Lys Val Ala Gly Asn Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  
Asn Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Ile Asp Ala Asn 
                85                  90                  95      
Phe Phe Cys Arg Leu Asn Asp Ser Phe Tyr Val Asp Ser Glu Lys Arg 
            100                 105                 110         
Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
                165                 170                 175     
Lys Asn Thr Ser Val Asp Gly Val Tyr Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         
Asn Gln Val Phe Ala Ser Asn Ile Lys Leu Glu Glu Asn Lys Asp Val 
        195                 200                 205             
Ala Lys Ile Leu Ala Glu Lys Val Thr Arg Lys Glu Lys Leu Glu Arg 
    210                 215                 220                 
Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser Thr Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Ile Ser Leu Ile Val Gly Ser Lys Gly Asn Phe Gln Lys Val Phe 
                245                 250                 255     
Asp Leu Val Glu Lys Thr Asp Ile Glu Cys Ala Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Ile Ile Gly Asp Glu Tyr Ala Glu 
        275                 280                 285             
Leu Phe Val Ala Ala Lys Asn Ala Tyr Asn Ala Val Val Leu Ser Ser 
    290                 295                 300                 
Ile Ile Thr Val Thr Asp Thr Glu Thr Asn Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Asp Thr His Glu Lys Asp Leu Ser Glu Leu Lys 
                325                 330                 335     
Ala Phe Ile Lys Leu His Leu Pro Lys Gln Tyr Glu Glu Ile Phe Ser 
            340                 345                 350         
Asn Val Glu Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Lys 
        355                 360                 365             
Gln Ala Asp Phe Tyr Lys Tyr Met Lys Thr Met Leu Glu Asn Ile Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Ile Ala Lys Ile Glu Glu Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile Pro His Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Glu Ala Ile Leu His Gln Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Tyr Asp Lys Ile Lys Ser Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly Gln Ser Glu Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Lys Val Asp Phe Gly Lys Ser Ala Val Asp Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Gln Gly Lys Thr Asn Tyr Phe Ser Gly Gln Glu Lys 
    530                 535                 540                 
Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Lys Arg Lys Val Lys Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Gln Phe Leu Arg Asn Met Ser His His Ile Glu Ser 
                565                 570                 575     
Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn Ser Ser Tyr Ala Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Val Gly Ile Lys Gln Glu Ile Leu Asp Asn 
        595                 600                 605             
Pro Leu Asn Thr Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu Gln Gln Phe Ser Asp 
625                 630                 635                 640 
Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Val Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe Lys 
    690                 695                 700                 
Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Ala Asp Lys Asp Ile Gln 
705                 710                 715                 720 
Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Ser Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Gly Lys Gly Lys Asn Asn Ser Arg Pro Arg Tyr Lys Ser Leu Glu Lys 
    770                 775                 780                 
Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Glu Leu Arg Asn Asn Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala Gly Asn Arg Glu Lys 
    850                 855                 860                 
Gly Asp Asp Val Pro Pro Leu Glu Ile Val Arg Lys Arg Lys Val Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Gln Gly Asn Leu Met Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Tyr Glu Lys Asp Asp His 
    930                 935                 940                 
Gly Asn Thr Met Glu Gln Val Arg Ile Val Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr Lys Val Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Asn Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr His Gln Phe Asp Trp Phe Lys Ala Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Thr Asn Ile Met Leu Phe Phe Ala Gln Lys Asp 
1025                1030                1035                1040
Arg Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Tyr 
                1045                1050                1055    
Leu Asp Thr Ile Lys Lys Val Leu Ser Tyr Arg Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Ile Gln Lys Gly Glu Phe Ser Lys Ala Thr Ile Lys 
        1075                1080                1085            
Pro Lys Gly Asn Ser Ser Lys Leu Ile Pro Arg Lys Thr Asn Trp Asp 
    1090                1095                1100                
Pro Met Lys Tyr Gly Gly Leu Asp Ser Pro Asn Met Ala Tyr Ala Val 
1105                1110                1115                1120
Val Ile Glu Tyr Asp Ile Ala Lys Gly Lys Lys Lys Lys Ile Val Phe 
                1125                1130                1135    
Glu Lys Lys Ile Ile Arg Ile Thr Ile Met Glu Arg Lys Ala Phe Glu 
            1140                1145                1150        
Lys Asp Glu Lys Ala Phe Leu Glu Glu Gln Gly Tyr Arg Gln Pro Lys 
        1155                1160                1165            
Glu Asp Val Leu Thr Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Cys Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Asn Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Gln Val Leu Pro Asn His Leu Val Thr Leu Leu His His Ala 
                1205                1210                1215    
Lys Asn Cys Glu Ala Ser Asn Asp Gly Lys Ser Leu Asp Tyr Ile Glu 
            1220                1225                1230        
Ser Asn Arg Glu Met Phe Ala Glu Leu Leu Ala His Val Ser Glu Phe 
        1235                1240                1245            
Ala Lys Arg Tyr Thr Leu Ala Asp Ala Asn Leu Asn Lys Ile Asn Gln 
    1250                1255                1260                
Leu Phe Glu Gln Asn Lys Glu Gly Asp Ile Lys Val Ile Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Ala Phe Asn Ala Met Gly Ala Pro Ala Ser Phe 
                1285                1290                1295    
Lys Phe Phe Glu Thr Thr Ile Glu Arg Lys Arg Tyr Asn Asn Leu Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Ala Arg Lys Arg Leu Asp Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 38
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #90

<400> 38
Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          
Lys Val Ala Gly Asn Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  
Asn Arg Thr Thr Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Ala Glu Met Ser Glu Val Asp Ala Asn 
                85                  90                  95      
Phe Phe Tyr Arg Leu Glu Asp Ser Phe Tyr Ile Glu Ser Glu Lys Arg 
            100                 105                 110         
His Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Lys Leu Val Asn 
    130                 135                 140                 
Ser Ser Asp Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Lys Leu Asp Thr 
                165                 170                 175     
Lys Asn Thr Ser Val Asp Glu Val Phe Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         
Asn Gln Val Phe Ala Ser Asp Ile Arg Leu Glu Glu Asn Lys Glu Val 
        195                 200                 205             
Ala Lys Ile Leu Ser Glu Lys Leu Thr Arg Arg Glu Lys Leu Asp Lys 
    210                 215                 220                 
Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser Thr Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Ile Ser Leu Ile Ile Gly Ser Lys Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Asp Glu Lys Thr Asp Ile Glu Cys Ala Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Glu 
        275                 280                 285             
Ile Phe Val Ala Ala Lys Asn Ala Tyr Asn Ala Val Val Leu Ser Ser 
    290                 295                 300                 
Ile Ile Thr Val Thr Asp Thr Glu Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Asp Lys His Glu Lys Asp Leu Lys Arg Leu Lys 
                325                 330                 335     
Ala Phe Phe Lys Val His Leu Pro Glu Lys Phe Asn Glu Val Phe Asn 
            340                 345                 350         
Asp Val Glu Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Lys 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Met Lys Lys Met Leu Glu Asn Ile Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Ile Asp Gln Ile Glu Glu Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile Pro His Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Glu Ala Ile Leu His Gln Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asp Tyr Glu Lys Ile Lys Ser Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly Gln Ser Glu Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Lys Val Asp Phe Gly Lys Ser Ala Ile Asp Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn Glu Leu Thr Lys Ile Arg 
        515                 520                 525             
Tyr Ile Asp Asp Gln Gly Lys Thr His Tyr Phe Ser Gly Gln Glu Lys 
    530                 535                 540                 
Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Lys Arg Lys Val Lys Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Gln Phe Leu Tyr Asn Met Ile Asn His Ile Glu Ser 
                565                 570                 575     
Pro Thr Ile Glu Gly Val Glu Asp Ala Phe Asn Ser Ser Phe Ala Thr 
            580                 585                 590         
Tyr His Asp Leu Gln Lys Val Gly Val Thr Gln Glu Ile Leu Asp Asn 
        595                 600                 605             
Pro Leu Asn Ala Glu Met Leu Glu Glu Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu Gln Gln Phe Ser Asp 
625                 630                 635                 640 
Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Ile Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe Lys 
    690                 695                 700                 
Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Ala Asp Lys Asp Ile Gln 
705                 710                 715                 720 
Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Gly Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Gly Lys Gly Lys Asn Asn Ser Lys Pro Arg Phe Thr Ser Leu Glu Lys 
    770                 775                 780                 
Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Gly Leu Lys Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Ala Asn Arg Glu Lys 
    850                 855                 860                 
Gly Asp Asn Val Pro Ser Leu Glu Val Val Arg Lys Arg Lys Val Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Gln Ala Lys Leu Met Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Cys Lys Lys Asp Glu Asn 
    930                 935                 940                 
Gly Asn Val Ile Glu Gln Val Arg Ile Val Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr Lys Val Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Asn Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr His Gln Phe Asp Trp Phe Lys Ala Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Thr Asn Ile Met Leu Phe Phe Ala Lys Lys Asp 
1025                1030                1035                1040
Arg Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Tyr 
                1045                1050                1055    
Leu Asp Thr Ile Lys Lys Val Leu Ser Tyr Arg Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Ile Gln Lys Gly Glu Phe Ser Asn Ala Thr Val Asn 
        1075                1080                1085            
Pro Lys Gly Asn Ser Ser Lys Leu Ile Pro Arg Lys Ala Asp Trp Asp 
    1090                1095                1100                
Pro Ile Lys Tyr Gly Gly Phe Asp Gly Ser Asn Met Ala Tyr Ala Ile 
1105                1110                1115                1120
Val Ile Glu Tyr Asp Ile Glu Lys Arg Lys Lys Lys Thr Val Ile Lys 
                1125                1130                1135    
Glu Lys Glu Leu Ile Gln Ile Asn Ile Met Glu Arg Thr Ala Phe Glu 
            1140                1145                1150        
Lys Asp Gln Lys Ala Phe Leu Glu Glu Lys Gly Tyr Arg Asn Pro Lys 
        1155                1160                1165            
Glu Asp Val Leu Thr Lys Ile Pro Lys Tyr Thr Leu Tyr Glu Cys Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Gly Ser Ala Asn Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Asn His Leu Met Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Asn Cys Glu Ala Ser Asn Asp Gly Lys Ser Leu Ala Tyr Ile Glu 
            1220                1225                1230        
Ser His Arg Glu Met Phe Ala Glu Leu Leu Ala His Ile Ser Glu Phe 
        1235                1240                1245            
Ala Lys Arg Tyr Thr Leu Ala Asp Ala Asn Leu Glu Lys Ile Asn Thr 
    1250                1255                1260                
Leu Phe Glu Gln Asn Lys Glu Gly Asp Ile Lys Val Ile Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Ala Phe Asn Ala Met Gly Ala Pro Ala Ser Phe 
                1285                1290                1295    
Lys Phe Phe Glu Thr Thr Ile Glu Arg Lys Arg Tyr Asn Asn Leu Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Ala Arg Lys Arg Leu Asp Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 39
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #93

<400> 39
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
Tyr Glu Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe His Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asp Gln Leu Asp Thr Glu Ala Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Gln Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Asn Asp Glu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His His Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Glu Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Glu Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asn Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Glu Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Phe Leu Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     
Val Asp Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Glu Lys Arg Leu Ala Thr Tyr Ala Asp 
625                 630                 635                 640 
Leu Phe Asp Lys Lys Val Leu Lys Gln Leu Lys Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Val Ser Gly Gln Thr Asp Ser Leu His 
705                 710                 715                 720 
Glu Gln Ile Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Thr Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ala Lys Gly Ile Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu Glu 
    770                 775                 780                 
Val Leu Lys Lys Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val Glu 
785                 790                 795                 800 
Asn Thr Gln Leu Gln Asn Glu Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asn Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Glu Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Lys Lys Met Lys Ser Tyr 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ser Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Pro Ile Arg Asn Val Lys Val Ile Thr Leu Lys Ser Lys Leu 
945                 950                 955                 960 
Val Ser Asp Phe Arg Lys Asp Phe Gly Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys Lys Tyr Asp Asp Lys Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asn Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Met Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Lys Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Ala Lys Gly Tyr Gln Asp Ile Gln 
        1155                1160                1165            
Glu Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Thr Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Phe Leu Tyr His Ala 
                1205                1210                1215    
Ser Arg Ile Asp Lys Ser Thr Ser Ser Glu Asn Leu Glu Tyr Val Glu 
            1220                1225                1230        
Gln His Lys His Glu Phe Asp Glu Ile Leu Asp Tyr Ile Ile Asp Phe 
        1235                1240                1245            
Ser Glu Arg Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ser 
    1250                1255                1260                
Leu Phe Asn Gln Asn Asp Asp Ser Asp Ile Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Leu Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Val Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp 
    1330                1335                1340

<210> 40
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #94

<400> 40
Met Glu Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Lys Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Ser Asp Val Asp Lys Leu Phe Tyr Gln Leu Val Gln Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Leu Asp Ala Ser Gly Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Gln Phe Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Ala Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Glu Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Phe Leu Ser Gly Glu Gln Lys 
    530                 535                 540                 
Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     
Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala His 
625                 630                 635                 640 
Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu His 
705                 710                 715                 720 
Glu Gln Ile Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Thr Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Thr Lys Gly Leu Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu Glu 
    770                 775                 780                 
Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val Glu 
785                 790                 795                 800 
Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu Ser 
            820                 825                 830         
Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn Tyr 
865                 870                 875                 880 
Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ala Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Pro Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys Leu 
945                 950                 955                 960 
Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys Val Tyr Asp Glu Gln Glu Ile Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Arg Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asn Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Met Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Arg Asn Ser Asp Lys Leu Ile Pro Arg Lys Lys Asp Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Lys Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Ser 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Gly Phe Leu Glu Ala Lys Gly Tyr Lys Asp Ile Gln 
        1155                1160                1165            
Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Thr Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Phe Leu Tyr His Ala 
                1205                1210                1215    
Ser Lys Ile Glu Lys Ser Thr Ser Ser Glu Asn Leu Ala Tyr Ile Glu 
            1220                1225                1230        
Gln His Lys His Glu Phe Asp Glu Ile Leu Glu Tyr Ile Ile Asp Phe 
        1235                1240                1245            
Ser Glu Arg Tyr Ile Leu Ala Asp Lys Asn Leu Ser Lys Val Lys Ser 
    1250                1255                1260                
Leu Phe Asn Lys His Glu Asp Ser Asp Ile Ser Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Leu Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp 
    1330                1335                1340

<210> 41
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #96

<400> 41
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Lys Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Glu Glu Ser Phe Leu Val Pro Glu Asp Lys Glu 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe His Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Leu Leu Phe Glu Glu Asp Pro Leu Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Gln Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Gly Leu Ser Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Ser Asp Glu Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Lys Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Gln Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Phe Leu Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asn Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     
Val Asp Ile Thr Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             
Glu Glu Asn Gln Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Lys Lys Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala Asp 
625                 630                 635                 640 
Leu Phe Asp Lys Ser Val Leu Lys Lys Leu Lys Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Ser Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Val Ile Gly Lys Thr Glu Ser Leu Lys 
705                 710                 715                 720 
Glu Val Val Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Thr Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ala Gln Gly Ile Lys Asn Ser Arg Gln Arg Met Lys Lys Leu Glu Glu 
    770                 775                 780                 
Val Leu Lys Lys Leu Gly Ser Asn Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Thr Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Asp Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Thr Ser Gln Glu Ala Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys Ser Tyr 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Lys Ala Gly Ala Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ser Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Leu Asp Glu Asn 
    930                 935                 940                 
Asn Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Lys Leu 
945                 950                 955                 960 
Val Ser Asp Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys Lys Tyr Asp Asp Lys Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asn Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Ile Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Lys Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Ala Lys Gly Tyr Gln Asp Ile Gln 
        1155                1160                1165            
Glu His Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Leu Val Leu Pro Ala His Tyr Val Thr Phe Leu Tyr Leu Ala 
                1205                1210                1215    
Ser Arg Tyr Asp Lys Leu Thr Ser Ser Glu Asp Leu His Phe Val Glu 
            1220                1225                1230        
Gln His Leu His Tyr Phe Asp Glu Ile Leu Asp Ile Ile Ile Asp Phe 
        1235                1240                1245            
Ser Glu Arg Tyr Ile Leu Ala Asp Ser Asn Leu Glu Lys Ile Lys Ser 
    1250                1255                1260                
Leu Tyr Asn Gln Asn Asp Asp Phe Ser Ile Asn Glu Gln Ala Ser Asn 
1265                1270                1275                1280
Met Ile Asn Leu Phe Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Ala Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Val Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp 
    1330                1335                1340

<210> 42
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #97

<400> 42
Met Arg Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Lys Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Asn Ile Phe Ser Asp Glu Met Ala Lys Val Asp Asp Ala 
                85                  90                  95      
Phe Phe Gln Arg Leu Ser Glu Ser Phe Leu Val Pro Glu Asp Lys Glu 
            100                 105                 110         
Phe Asp Arg His Pro Ile Phe Gly Asn Leu Lys Asp Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Asp Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Leu Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Leu Leu Phe Glu Glu Asp Pro Ile Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Thr Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Glu Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Gly Leu Ser Glu Asp Ala Lys Leu Gln Ile Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Ser Asp Glu Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Val Lys Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Lys Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asn Lys Glu Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Leu Leu Ala Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Asn Glu Leu His Ala Ile Leu Arg Arg Gln Glu Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Ser Phe Ala Trp 
    450                 455                 460                 
Leu Lys Arg Arg Ser Asp Glu Ser Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Gln Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Pro Leu Tyr Glu Met Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Phe Leu Ser Ala Glu Gln Lys 
    530                 535                 540                 
Glu Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asn Tyr Phe Ser Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     
Val Asp Ile Thr Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Ala Phe Leu Asp Asp 
        595                 600                 605             
Glu Ala Asn Gln Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Glu Lys Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala Asp 
625                 630                 635                 640 
Leu Phe Asp Lys Ser Val Leu Lys Lys Leu Lys Lys Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Ser Leu Asp Phe Ala 
    690                 695                 700                 
Lys Ile Ile Glu Asn Ala Gln Glu Lys Ala Lys Ser Glu Ser Leu Lys 
705                 710                 715                 720 
Glu Thr Val Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Ile Lys Ile Val Asp Glu Ile Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ala Gln Gly Ile Lys Asn Ser Arg Gln Arg Leu Lys Lys Leu Glu Glu 
    770                 775                 780                 
Val His Lys Lys Thr Gly Ser Lys Ile Leu Lys Glu His Ser Val Ser 
785                 790                 795                 800 
Asn Thr Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Leu Leu Gln Asp 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Asp Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Ile Val Leu Thr Ser Gln Glu Ala Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys Ser Tyr 
865                 870                 875                 880 
Trp Gln Lys Gln Leu Lys Ser Gly Ala Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
His Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ser Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Ser Lys Leu Asp Glu Asn 
    930                 935                 940                 
Asn Lys Ser Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Lys Met 
945                 950                 955                 960 
Val Ser Asp Phe Arg Lys Asp Phe Gly Leu Tyr Lys Leu Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Ala Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys His Tyr Asp Asp Ser Ser Leu Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Lys Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Val Lys Asp 
                1045                1050                1055    
Phe Lys Thr Ile Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Ile Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Lys Val Ala Lys Gly Lys Ala Gln Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Gln Ser Glu Phe Glu 
            1140                1145                1150        
Lys Asp Pro Ile Ala Phe Leu Glu Lys Lys Gly Tyr Gln Asp Ile Gln 
        1155                1160                1165            
Glu Asn Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Leu Ala Leu Pro Asn Lys Tyr Val Lys Phe Leu Tyr Leu Ala 
                1205                1210                1215    
Ser Arg Tyr Asp Lys Leu Thr Lys Ser Glu Asp Arg His Phe Val Glu 
            1220                1225                1230        
Gln His Leu His Tyr Phe Asp Glu Ile Leu Asp Ile Ile Val Asp Phe 
        1235                1240                1245            
Ser Asn Arg Tyr Ile Leu Ala Asp Ser Asn Leu Glu Lys Ile Lys Ser 
    1250                1255                1260                
Leu Tyr Asn Glu Lys Asp Asp Phe Ser Ile Asn Glu Gln Ala Thr Asn 
1265                1270                1275                1280
Met Leu Asn Leu Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Ala Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Asn 
            1300                1305                1310        
Glu Val Leu Asn Ser Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Gly Asp 
    1330                1335                1340

<210> 43
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #98

<400> 43
Met Arg Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Lys 
65                  70                  75                  80  
Tyr Leu Gln Asn Ile Phe Ser Asp Glu Met Ser Lys Val Asp Ser Ala 
                85                  90                  95      
Phe Phe Gln Arg Leu Ser Glu Ser Phe Leu Val Pro Asp Asp Lys Glu 
            100                 105                 110         
Phe Asp Arg His Pro Ile Phe Gly Asn Leu Lys Asp Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Asp Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asp Ser 
                165                 170                 175     
Glu Asn Thr Asp Val His Ala Leu Phe Leu Lys Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Leu Leu Phe Glu Glu Asp Lys Ile Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Thr Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Glu Ile Pro Asn Gln Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ser Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Glu Leu Ser Glu Asp Ala Lys Leu Gln Ile Ser Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Ile Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Lys Gly Ala Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Val Gln Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Lys Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Asp 
            340                 345                 350         
Asn Lys Glu Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Lys Leu Ile Ser Lys Leu Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Asn Glu Leu Lys Ala Ile Ile Arg Arg Gln Glu Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Leu Phe Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Ser Phe Ala Trp 
    450                 455                 460                 
Leu Lys Arg Arg Ser Asn Glu Ser Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Gln Glu Ala Ser Ala Arg Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Pro Leu Tyr Glu Met Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Gln Thr Glu Gly Met Lys Lys Pro Phe Leu Ser Ser Glu Glu Lys 
    530                 535                 540                 
Glu Glu Ile Val Asn Leu Leu Phe Lys Lys Glu Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asn Tyr Phe Ser Lys Ile Lys Cys Phe Asp Ser 
                565                 570                 575     
Val Thr Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Ala Phe Leu Asp Asp 
        595                 600                 605             
Glu Ala Asn Gln Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Glu Lys Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala Asp 
625                 630                 635                 640 
Val Phe Asp Lys Ser Val Leu Lys Lys Leu Lys Lys Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Gln Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Thr Gly Lys Thr Ile Leu Asp Phe Leu Lys Asp Asp Gly Val Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Ser Leu Asp Phe Ala 
    690                 695                 700                 
Lys Ile Ile Lys Asn Ala Gln Glu Lys Thr Lys Ser Glu Ser Leu Glu 
705                 710                 715                 720 
Glu Thr Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Ile Lys Ile Val Asp Glu Ile Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Ser Thr 
        755                 760                 765             
Ala Gln Gly Ile Lys Asn Ser Arg Gln Arg Leu Arg Lys Leu Glu Glu 
    770                 775                 780                 
Val His Lys Asn Thr Gly Ser Lys Ile Leu Lys Glu His Asn Val Ser 
785                 790                 795                 800 
Asn Thr Gln Leu Gln Ser Asp Arg Leu Tyr Leu Tyr Leu Leu Gln Asp 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Lys Asp Leu Asp Phe Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Ile Val Leu Thr Ser Gln Glu Ser Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Ile Val Asn Lys Met Lys Ser Tyr 
865                 870                 875                 880 
Trp Gln Lys Gln Leu Lys Ser Gly Ala Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
His Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Phe Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Ser Asn Leu Asp Glu Asn 
    930                 935                 940                 
Ser Lys Ser Asn Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Lys Met 
945                 950                 955                 960 
Val Ser Asp Phe Arg Lys Asp Phe Gly Phe Tyr Lys Leu Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Ala Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys His Tyr Asp Asp Ser Ser Leu Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Lys Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Val Lys Asp 
                1045                1050                1055    
Ile Lys Thr Ile Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Ile Ile Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Lys Val Ala Lys Gly Lys Ala Gln Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Gln Asn Glu Phe Glu 
            1140                1145                1150        
Lys Asp Pro Ile Ala Phe Leu Glu Lys Lys Gly Tyr Gln Asp Ile Gln 
        1155                1160                1165            
Glu Ser Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Lys Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Leu Ala Leu Pro Asn Lys Tyr Val Lys Phe Leu Tyr Leu Ala 
                1205                1210                1215    
Ser Arg Tyr Thr Lys Phe Thr Lys Glu Glu Asp Arg His Phe Val Glu 
            1220                1225                1230        
Ser His Leu His Tyr Phe Asp Glu Ile Met Asp Ile Ile Val Asp Phe 
        1235                1240                1245            
Ser Asn Arg Tyr Ile Leu Ala Asp Ser Asn Leu Glu Lys Ile Gln Asn 
    1250                1255                1260                
Leu Tyr Asn Glu Lys Asp Asn Phe Ser Ile Glu Glu Gln Ala Thr Asn 
1265                1270                1275                1280
Met Leu Asn Leu Phe Thr Phe Thr Gly Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asn Ala Asp Ile Asp Arg Lys Arg Tyr Thr Ser Thr Asn 
            1300                1305                1310        
Glu Ile Leu Asn Ser Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Gly Asp 
    1330                1335                1340

<210> 44
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #99

<400> 44
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Glu Ser His Leu Ser Glu Gln Thr Val Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu Asn Leu Pro Asp Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Lys Thr Gln Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Leu Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Leu Asp Tyr Leu Glu Lys Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Leu Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu Lys 
705                 710                 715                 720 
Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Asp 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys Lys Tyr Asn Tyr Arg Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Lys Lys Gly Tyr Gln Asn Ile Gln 
        1155                1160                1165            
Glu Asp Asn Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Asp Lys Ser Asn Glu Pro Glu His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Lys Asn Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Leu Tyr Asp Gln Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Val Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 45
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #100

<400> 45
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Ile Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Glu Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asn Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu Asn Val Pro Asp Gln Tyr Asn Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Ser Tyr Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Ser Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ala Gln Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             
Glu Ala Asn Glu Glu Ile Ile Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Val Asp Asp Leu Ala 
705                 710                 715                 720 
Gln Val Val His Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Gln Asp 
    770                 775                 780                 
Ser Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu Lys Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Ala Arg Lys Ser Phe 
865                 870                 875                 880 
Trp Val Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Val Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Lys Lys Tyr Asn His Arg Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Lys Lys Gly Tyr His Asn Ile Gln 
        1155                1160                1165            
Glu Asp Lys Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Gln Arg Ile Asp Lys Ser Asn Glu Pro Glu His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Lys Asn Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Val Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ala 
    1250                1255                1260                
Leu Tyr Asp Gln Asn Asp Asp Ala Asp Ile Glu Glu Ile Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Cys Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 46
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #101

<400> 46
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Val Asp Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Ile Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Glu Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Val Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Val Asn Ala Pro Asp Gln Tyr Asn Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ala Lys Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             
Lys Ala Asn Glu Glu Ile Ile Glu Asp Ile Ile Gln Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Gln Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Asp Val Asp Asp Ile Ala 
705                 710                 715                 720 
Asn Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Gln Asp 
    770                 775                 780                 
Ser Leu Lys Asn Leu Gly Ser Asn Ile Asn Lys Lys Lys Pro Val Glu 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Ile Glu Val Val Arg Ala Arg Lys Ser Phe 
865                 870                 875                 880 
Trp Val Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Val Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Lys Lys Tyr Asn His Arg Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asn Lys Gln Lys Asp 
                1045                1050                1055    
Phe Glu Ile Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Lys Lys Gly Tyr His Asn Ile Gln 
        1155                1160                1165            
Glu Asp Lys Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
His Arg Ile Asp Lys Ser Asn Glu Pro Glu His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Lys Asn Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Val Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ala 
    1250                1255                1260                
Leu Tyr Asp Gln Asn Asp Asp Ala Ser Ile Glu Glu Ile Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Asn Phe Phe Gly Glu Lys Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Cys Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 47
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #102

<400> 47
Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Asp Glu Asp Lys Arg 
            100                 105                 110         
Gly Glu Arg His Pro Ile Phe Gly Asn Ile Ala Ala Glu Val Lys Tyr 
        115                 120                 125             
His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Thr Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Lys Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Ala Leu Phe Lys Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Lys Thr Ile Glu Glu Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Leu Ser Ile Leu Thr Glu Lys Val Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Gly Leu Leu Gly Glu Val Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Ala Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Val Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Lys Lys Leu Lys 
                325                 330                 335     
Asp Phe Ile Lys Val Asn Ala Pro Asp Gln Tyr Asn Ala Ile Phe Lys 
            340                 345                 350         
Asp Lys Asn Lys Lys Gly Tyr Ala Gly Tyr Ile Glu Ser Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Leu Lys Ile Asn 
    370                 375                 380                 
Gly Ser Gly Asp Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Lys Ala Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Ile Leu Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Glu Ala Lys Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Asp Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     
Val Asn Leu Thr Gly Leu Asp Lys Glu Phe Asn Ser Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Arg Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             
Lys Ala Asn Glu Gln Ile Ile Glu Asp Ile Ile Gln Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Gln Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Lys Ala Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Asn Lys Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Tyr Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Ala Lys Ala Gln Ile Ile Gly Asp Val Asp Asp Ile Ala 
705                 710                 715                 720 
Asn Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Ala Asn Ile Ile Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asp Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Leu Leu Gln Asp 
    770                 775                 780                 
Ser Leu Lys Asn Leu Asp Asn Pro Val Asn Ile Lys Asn Pro Val Glu 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asn Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Ile Glu Val Val Arg Ala Arg Lys Ser Phe 
865                 870                 875                 880 
Trp Met Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Glu Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu His Asp Glu Asn 
    930                 935                 940                 
Lys Lys Val Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Lys Lys Tyr Asn Tyr Arg Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Lys Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Asp Gly Glu Ile Ala Trp Asn Lys Gln Lys Asp 
                1045                1050                1055    
Phe Lys Ile Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Ser Tyr Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Gln Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Lys Lys Gly Tyr Arg Asn Ile Arg 
        1155                1160                1165            
Glu Asp Lys Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Leu Val Ile Pro Gln Arg Phe Thr Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
His Arg Ile Asp Lys Ser Asn Glu Pro Glu His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Lys Asp Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Val Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ala 
    1250                1255                1260                
Leu Phe Ala Gln Asn Asp Asp Ala Ser Ile Glu Glu Ile Ala Thr Ser 
1265                1270                1275                1280
Phe Ile Ser Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Asn Phe Phe Gly Glu Lys Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Cys Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 48
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #103

<400> 48
Met Lys Lys Ser Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Val Asn Lys Ser Glu Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Arg Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ser Ser Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     
Asn Asp Leu Ile Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             
Lys Thr Asn Glu Glu Ile Ile Glu Asp Ile Ile Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Lys Gln Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Gln Ile Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp Ile Glu 
705                 710                 715                 720 
Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Asp 
            740                 745                 750         
Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Arg Ser Lys Ser Gln Gln Arg Leu Lys Arg Leu Gln Asp 
    770                 775                 780                 
Ser Leu Lys Glu Leu Gly Ser Asn Leu Asn Lys Lys Lys Pro Val Glu 
785                 790                 795                 800 
Asn Ser His Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp His Leu Ser 
            820                 825                 830         
Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Ile Glu Ile Val Arg Ala Arg Lys Ser Tyr 
865                 870                 875                 880 
Trp Val Lys Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Lys Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Lys Lys Tyr Asp His Ser Glu Met Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Tyr Ala Asp Asp Thr Gly Glu Ile Val Trp Asn Lys Gln Lys Asp 
                1045                1050                1055    
Phe Glu Ile Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Val Tyr Trp Asp 
    1090                1095                1100                
Thr Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Ser Ile Met Glu Arg Ser Phe Phe Glu 
            1140                1145                1150        
Glu Asn Pro Val Ala Phe Leu Glu Lys Lys Gly Tyr His Asn Val Gln 
        1155                1160                1165            
Glu Asp Lys Leu Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Phe Glu 
    1170                1175                1180                
Gly Gly Arg Arg Arg Leu Leu Ala Ser Ala Thr Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Glu Leu Leu Tyr His Ala 
                1205                1210                1215    
His Arg Ile Asp Asn Phe Asn Ser Thr Glu His Leu Lys Tyr Val Ser 
            1220                1225                1230        
Glu His Lys Lys Glu Phe Glu Glu Val Leu Ser Cys Val Glu Asn Phe 
        1235                1240                1245            
Ala Asn Leu Tyr Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala 
    1250                1255                1260                
Ala Ala Asp Ser Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Asn Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Asn Phe Leu Gly Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Cys Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Glu 
    1330                1335                1340

<210> 49
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #104

<400> 49
Met Glu Lys Ser Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Lys Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Glu Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Pro Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys Gln Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Val Asn Lys Leu Glu Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Ser Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Ala Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Lys Tyr His Ser Asp Glu Pro Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ser Phe Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Ala Lys Leu Leu Ser Tyr Leu Asn Asn Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     
Asn Asp Leu Ile Gly Leu Asp Lys Asp Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             
Lys Thr Asn Glu Gln Ile Ile Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Ser Gln Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Asn Asn Lys Thr Ile Leu Asp Phe Leu Ile Asp Asp Gly His Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe Lys 
    690                 695                 700                 
Thr Ile Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp Ile Glu 
705                 710                 715                 720 
Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Asp 
            740                 745                 750         
Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Gly Tyr Gly Arg Asn Lys Ser Asn Gln Arg Leu Lys Arg Leu Gln Asp 
    770                 775                 780                 
Ser Leu Lys Glu Phe Gly Ser Asp Leu Ser Lys Lys Lys Pro Val Glu 
785                 790                 795                 800 
Asn Ser His Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp His Leu Ser 
            820                 825                 830         
Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Ile Glu Ile Val Arg Asn Arg Lys Ser Tyr 
865                 870                 875                 880 
Trp Tyr Lys Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Lys Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Lys Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Lys Lys Tyr Asp His Ser Glu Met Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Tyr Ala Asp Ala Asp Gly Glu Val Val Trp Asn Lys Gln Lys Asp 
                1045                1050                1055    
Phe Asp Ile Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Val Tyr Trp Asp 
    1090                1095                1100                
Thr Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Ser Ile Met Glu Arg Ser Phe Phe Glu 
            1140                1145                1150        
Glu Asn Pro Val Ser Phe Leu Glu Lys Lys Gly Tyr His Asn Val Gln 
        1155                1160                1165            
Glu Asp Lys Leu Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Phe Glu 
    1170                1175                1180                
Gly Gly Arg Arg Arg Leu Leu Ala Ser Ala Thr Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Val Val Leu Pro Ala His Leu Val Glu Leu Leu Tyr His Ala 
                1205                1210                1215    
His Arg Ile Asp Asn Phe Asn Ser Thr Glu His Leu Lys Tyr Val Ser 
            1220                1225                1230        
Glu His Lys Lys Glu Phe Glu Glu Val Leu Ser Cys Val Glu Asn Phe 
        1235                1240                1245            
Ala Asn Leu Tyr Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala 
    1250                1255                1260                
Ala Ala Glu Ser Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Ala Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Asn Phe Leu Gly Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Cys Leu Ser Ala Thr Leu Ile His Gln Ser Val Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Glu 
    1330                1335                1340

<210> 50
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #105

<400> 50
Thr Lys Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Ala Asn Lys Ser Glu Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Arg Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Lys 
465                 470                 475                 480 
Val Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Asp Ser Phe Phe Asp Ser Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu Tyr Arg Ile 
                565                 570                 575     
Lys Asp Leu Ile Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             
Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile Lys Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Lys Gln Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Gln Ile Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp Ile Glu 
705                 710                 715                 720 
Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Asp 
            740                 745                 750         
Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu Lys Lys Leu Gln Asn 
    770                 775                 780                 
Ser Leu Lys Glu Leu Gly Ser Asn Leu Asn Glu Glu Lys Pro Val Glu 
785                 790                 795                 800 
Asn Ser His Leu Gln Asn Asp Gln Leu Phe Leu Tyr Tyr Ile Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Asp Glu Leu Asp Ile Asp His Leu Ser 
            820                 825                 830         
Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Leu Asp Ile Val Arg Ala Arg Lys Ala Glu 
865                 870                 875                 880 
Trp Val Arg Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Lys Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Lys Lys Tyr Asp His Ser Glu Met Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Thr Val Val 
1025                1030                1035                1040
Lys Tyr Ala Asp Asp Thr Gly Glu Ile Ala Trp Asn Lys Gln Thr Asp 
                1045                1050                1055    
Phe Glu Lys Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Thr Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Val Tyr Trp Asp 
    1090                1095                1100                
Thr Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Ser Ile Met Glu Arg Ser Phe Phe Glu 
            1140                1145                1150        
Glu Asn Pro Val Glu Phe Leu Glu Asn Lys Gly Tyr His Asn Val Gln 
        1155                1160                1165            
Glu Asp Lys Leu Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Phe Glu 
    1170                1175                1180                
Gly Gly Arg Arg Arg Leu Leu Ala Ser Ala Thr Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Glu Leu Leu Tyr His Ala 
                1205                1210                1215    
His Arg Ile Asp Asn Phe Asn Ser Thr Glu His Leu Lys Tyr Val Ser 
            1220                1225                1230        
Glu His Lys Lys Glu Phe Glu Glu Val Leu Ser Cys Val Glu Asn Phe 
        1235                1240                1245            
Ala Asn Leu Tyr Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala 
    1250                1255                1260                
Ala Ala Asp Ser Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Asn Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Asn Phe Leu Gly Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Cys Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Glu 
    1330                1335                1340

<210> 51
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #106

<400> 51
Thr Lys Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Ile Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys Tyr Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Ala Asn Lys Ser Glu Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Asn Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Arg Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Lys 
465                 470                 475                 480 
Val Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Tyr Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Asp Ser Phe Phe Asp Ser Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu Tyr Arg Ile 
                565                 570                 575     
Lys Asp Leu Ile Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ala Phe Leu Asp Asp 
        595                 600                 605             
Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile Lys Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Lys Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Ala Asn Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Pro Phe Lys 
    690                 695                 700                 
Gln Ile Ile Gln Lys Ser Gln Val Val Gly Asp Val Asp Asp Ile Glu 
705                 710                 715                 720 
Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Asp 
            740                 745                 750         
Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu Lys Lys Leu Gln Asn 
    770                 775                 780                 
Ser Leu Lys Glu Leu Gly Ser Asn Leu Asn Glu Glu Lys Pro Val Glu 
785                 790                 795                 800 
Asn Ser His Leu Gln Asn Asp Gln Leu Phe Leu Tyr Tyr Ile Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Asp Glu Leu Asp Ile Asp His Leu Ser 
            820                 825                 830         
Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Leu Asp Ile Val Arg Ala Arg Lys Ala Glu 
865                 870                 875                 880 
Trp Val Arg Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Asp Phe Glu Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Ser Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Lys Lys Tyr Asp His Ser Glu Met Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Thr Val Val 
1025                1030                1035                1040
Lys Tyr Ala Asp Asp Thr Gly Glu Ile Ala Trp Asn Lys Gln Thr Asp 
                1045                1050                1055    
Phe Glu Lys Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Thr Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Val Tyr Trp Asp 
    1090                1095                1100                
Thr Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Phe Val Val Ala Asp Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Ser Ile Met Glu Arg Ser Phe Phe Glu 
            1140                1145                1150        
Glu Asn Pro Val Glu Phe Leu Glu Asn Lys Gly Tyr His Asn Ile Arg 
        1155                1160                1165            
Glu Asp Lys Leu Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Phe Glu 
    1170                1175                1180                
Gly Gly Arg Arg Arg Leu Leu Ala Ser Ala Ser Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Gly His Leu Val Glu Leu Leu Tyr His Ala 
                1205                1210                1215    
His Arg Ile Asp Asn Phe Asn Ser Thr Glu Tyr Leu Asn Tyr Val Ser 
            1220                1225                1230        
Glu His Lys Lys Glu Phe Glu Lys Val Leu Ser Cys Val Glu Asp Phe 
        1235                1240                1245            
Ala Asn Leu Tyr Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala 
    1250                1255                1260                
Val Ala Asp Ser Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Asn Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Asn Phe Leu Gly Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Cys Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Glu 
    1330                1335                1340

<210> 52
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #107

<400> 52
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Val Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Leu Leu Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Phe Ile Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Ser Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Gln Asn Leu Pro Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Asn 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Lys Thr Tyr Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Leu Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Leu Asp Tyr Leu Glu Lys Glu Phe Asp Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Leu Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu Lys 
705                 710                 715                 720 
Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Asp 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Ala Arg Phe Asn Thr Glu Leu Asp Glu Asn 
    930                 935                 940                 
Asn Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Pro Lys Tyr Asn Tyr Arg Glu Arg Lys Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Lys Lys Gly Tyr Gln Asn Ile Gln 
        1155                1160                1165            
Glu Asp Asn Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile His Lys Val Asn Glu Pro Glu His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Lys Asn Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Asn Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Leu Tyr Asp Gln Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Ile Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 53
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #108

<400> 53
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Glu Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Val Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Leu Leu Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Phe Ile Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asp Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Gln Asn Leu Pro Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Ala Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Asn 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Arg Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Lys Thr Tyr Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Gly Leu Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Leu Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp Asp 
        595                 600                 605             
Ser Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Leu Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu Lys 
705                 710                 715                 720 
Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Glu Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Asp 
    770                 775                 780                 
Ala Ile Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Arg Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Asp Val Val Arg Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Leu Asp Glu Asn 
    930                 935                 940                 
Asn Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Lys Ala Leu Leu Lys Lys Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Pro Lys Tyr Asn Tyr Arg Glu Arg Lys Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys His 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Arg Lys Gly Tyr Gln Asn Ile Gln 
        1155                1160                1165            
Glu Asp Asn Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile His Lys Val Asn Glu Pro Glu His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Lys Asn Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Leu Tyr Asp Gln Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Ile Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 54
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #109

<400> 54
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Val Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Ser His Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Gly Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Thr Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Glu Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Val Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Leu Leu Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Phe Ile Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Glu Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Glu Leu Glu Val Leu Leu Ala Gln Ile Gly Asp Asn Tyr Ala Glu 
        275                 280                 285             
Leu Phe Leu Ser Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Val Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asn Glu His Gln Met Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Gln Lys Leu Ser Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Val Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
        355                 360                 365             
Gln Glu Ala Phe Tyr Lys Tyr Leu Lys Gly Leu Leu Asn Lys Ile Glu 
    370                 375                 380                 
Gly Ser Gly Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Arg Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro Phe 
            420                 425                 430         
Leu Ala Asp Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Ala Asp Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Ile Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asn Gln Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Lys Thr Glu Gln Gly Lys Thr Ala Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr Lys 
545                 550                 555                 560 
Asp Lys Leu Met Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Tyr Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Arg Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             
Ser Lys Asn Glu Lys Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Asn Tyr Ser Asp 
625                 630                 635                 640 
Leu Leu Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Glu Leu Ile His Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Asn Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu Asn 
705                 710                 715                 720 
Gln Val Val Ser Asp Ile Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Ile Met Gly His 
            740                 745                 750         
Gln Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Phe Thr 
        755                 760                 765             
Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Gly Leu Thr Asp 
    770                 775                 780                 
Ser Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro Val Glu 
785                 790                 795                 800 
Asn Ser Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Arg Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Tyr Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Lys Asp Val Val Arg Lys Met Lys Ser Tyr 
865                 870                 875                 880 
Trp Ser Lys Leu Leu Ser Ala Lys Leu Ile Thr Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Thr Asp Glu Asn 
    930                 935                 940                 
Asn Lys Lys Ile Arg Gln Val Lys Ile Val Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Ile Gly 
            980                 985                 990         
Lys Ala Leu Leu Gly Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Pro His Phe His Gly His Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Lys Asp Asp 
1025                1030                1035                1040
Val Arg Thr Asp Lys Asn Gly Glu Ile Ile Trp Lys Lys Asp Glu His 
                1045                1050                1055    
Ile Ser Asn Ile Lys Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Phe Tyr Trp Asp 
    1090                1095                1100                
Thr Lys Lys Tyr Gly Gly Phe Asp Ser Pro Ile Val Ala Tyr Ser Ile 
1105                1110                1115                1120
Leu Val Ile Ala Asp Ile Glu Lys Gly Lys Ser Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Asp Leu Val Gly Ile Thr Ile Met Glu Arg Thr Thr Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Arg Lys Gly Tyr Arg Asn Val Gln 
        1155                1160                1165            
Glu Glu Asn Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Arg Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Ile Val Leu Pro Asn His Leu Gly Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Asn Ile His Lys Val Asp Glu Pro Lys His Leu Asp Tyr Val Glu 
            1220                1225                1230        
Lys His Lys Asp Glu Phe Lys Glu Leu Leu Asp Val Val Ser Asn Phe 
        1235                1240                1245            
Ser Lys Lys Tyr Ile Leu Ala Glu Ser Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Leu Tyr Ala Gln Asn Asn Asn Glu Asp Ile Lys Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Ile Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Ile Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Gly Asp 
    1330                1335                1340

<210> 55
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #110

<400> 55
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Lys Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Leu Asp Val 
                165                 170                 175     
Arg Asn Thr Asp Val Gln Lys Leu Phe Gln Glu Phe Leu Glu Val Tyr 
            180                 185                 190         
Asp Thr Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Lys Leu Glu Arg 
    210                 215                 220                 
Leu Leu Ala Gln Phe Pro Asn Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Phe Leu Ala Leu Ile Val Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Val Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asp Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Ala Asn Leu Pro Asp Lys Tyr Lys Glu Val Phe Ser 
            340                 345                 350         
Asp Ala Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Asn 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His Leu 
                405                 410                 415     
Ala Glu Met Lys Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Asn Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Glu Thr Tyr Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Gly Leu Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Leu Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Ile Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp Asp 
        595                 600                 605             
Ser Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Leu Phe Thr Lys Lys Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Arg Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Asp Ser Asp Asn Leu His 
705                 710                 715                 720 
Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         
Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Ile Lys Asn Leu Gly Ser Asp Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Arg Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Asp Val Val Lys Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Leu Asp Glu Asn 
    930                 935                 940                 
Asn Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Asn Phe Arg Lys Glu Phe Glu Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Lys Ala Leu Leu Thr Lys Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Pro Lys Tyr Asn Tyr Arg Glu Arg Lys Ser Ala Thr Glu 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Lys Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys His 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Leu Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Arg Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Arg Lys Gly Tyr Gln Asn Ile Gln 
        1155                1160                1165            
Glu Asp Lys Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Lys Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Asn Lys Val Asn Glu Pro Glu His Ile Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Lys Asp Leu Leu Asn Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Leu Tyr Glu Gln Asn Glu Asp Asp Ser Val Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Val Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 56
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #111

<400> 56
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Ile Ala Asp Glu Val Lys Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Asp Asp Pro Leu Asp Val 
                165                 170                 175     
Arg Asn Thr Asp Ser Gln Lys Leu Phe Gln Glu Phe Leu Glu Ala Tyr 
            180                 185                 190         
Asp Ser Thr Gln Glu Asp Ser His Leu Ser Glu Gln Lys Ile Asp Ile 
        195                 200                 205             
Ser Asp Ile Ile Thr Glu Lys Ile Ser Lys Ser Arg Lys Val Glu Ala 
    210                 215                 220                 
Leu Leu Ala Gln Phe Pro Asn Gln Lys Lys Asn Ser Phe Phe Gly Asn 
225                 230                 235                 240 
Leu Val Ser Leu Val Phe Gly Leu Met Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Val Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asn Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asp Glu His Asn Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Asp Phe Ile Lys Ala Asn Leu Pro Asp Val Phe Lys Glu Val Phe Ser 
            340                 345                 350         
Asp Ala Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Gly Lys Val Asp 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Ile Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His Leu 
                405                 410                 415     
Ala Glu Met Lys Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Gln Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Ala Ser Phe Asn Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Ile Asp Lys Asn Ser Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Asn Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Thr Gly Glu Ala Arg Phe Phe Asp Ala Asn Leu Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Gly Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Leu Asp Phe Leu Glu Lys Asn Phe Asp Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Ile Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Tyr Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Glu Phe Met Asp Asp 
        595                 600                 605             
Ser Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Lys Tyr Ala Asp 
625                 630                 635                 640 
Leu Phe Asp Lys Lys Val Ile Asp Lys Leu Ala Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Arg Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Asp Glu Ile Ala Lys Ala Gln Val Ile Gly Asp Ser Asp Asp Leu His 
705                 710                 715                 720 
Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         
Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ala Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Ile Lys Asn Leu Gly Ser Asp Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Arg Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Glu Asp Val Val Lys Arg Met Lys Ser Phe 
865                 870                 875                 880 
Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Arg Glu Phe Asp Glu Asn 
    930                 935                 940                 
Asn Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Asn Phe Arg Lys Glu Phe Glu Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Lys Ala Leu Leu Thr Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Pro Lys Tyr Asn Tyr Arg Glu Arg Lys Ser Ala Thr Glu 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Met Phe Lys Thr Lys Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Ala Trp Asp Lys Thr Lys His 
                1045                1050                1055    
Phe Ala Thr Val Lys Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Ala Arg Lys Asn Tyr Leu Asp 
    1090                1095                1100                
Thr Gln Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Ile Gly Ile Thr Ile Met Glu Arg Ser Arg Phe Glu 
            1140                1145                1150        
Asn Asn Ser Val Ala Phe Leu Glu Arg Lys Gly Tyr Gln Asn Ile Gln 
        1155                1160                1165            
Glu Asn Lys Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Lys Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Ile Asn Ser Val Asn Glu Pro Glu His Ile Asn Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Lys Asp Leu Leu Asn Tyr Ile Arg Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Asn Glu 
    1250                1255                1260                
Leu Phe Glu Glu Asn Glu Glu Asp Ser Val Ser Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Val Leu Ser Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 57
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #112

<400> 57
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Met 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         
Gly Glu Arg His Pro Ile Phe Gly Asn Ile Ala Asp Glu Val Lys Tyr 
        115                 120                 125             
His Glu Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Asp Glu Pro Ile Asp Ile 
                165                 170                 175     
Arg Asn Thr Asp Ser Gln Lys Leu Phe Gln Glu Phe Leu Asp Ala Tyr 
            180                 185                 190         
Asp Ser Ile Gln Glu Asp Ser His Leu Ser Glu Glu Lys Thr Asp Ile 
        195                 200                 205             
Ser Asp Ile Ile Thr Ala Lys Ile Ser Lys Ser Arg Lys Val Glu Ala 
    210                 215                 220                 
Leu Leu Ala Gln Phe Pro Asn Gln Lys Lys Asn Ser Phe Phe Gly Asn 
225                 230                 235                 240 
Leu Val Ser Leu Val Phe Gly Leu Met Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Met Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Val Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Thr Asn Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Asp Glu His Asn Lys Asp Lys Gln Leu Leu Lys 
                325                 330                 335     
Asp Phe Ile Arg Ala Asn Ile Gly Asp Val Phe Lys Glu Val Phe Tyr 
            340                 345                 350         
Asp Thr Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Gly Lys Val Asp 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Ile Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His Leu 
                405                 410                 415     
Ala Glu Met Lys Ala Ile Leu Arg Arg Gln Gly Glu Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Gln Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Ser Phe Asn Ser Asn Glu Lys Ile Thr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Val Ile Asp Lys Ser Ser Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Asn Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Thr Gly Glu Ala Arg Phe Phe Asp Ala Asn Leu Lys 
    530                 535                 540                 
Lys Glu Ile Phe Asp Gly Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Leu Asp Phe Leu Glu Lys Asn Phe Asp Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Ile Thr Gly Leu Asp Asn Glu Phe Asn Ala Ser Tyr Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Glu Phe Met Asp Asp 
        595                 600                 605             
Ser Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Leu Lys Tyr Glu Asp 
625                 630                 635                 640 
Leu Phe Asp Lys Lys Val Ile Asp Lys Leu Ser Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Lys Asp Lys 
            660                 665                 670         
Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly His Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Asp Glu Ile Ala Lys Ala Gln Val Ile Gly Asp Gly Asp Asp Leu His 
705                 710                 715                 720 
Gln Val Val Gln Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         
Asp Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ala Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Gly Arg Leu Lys Glu 
    770                 775                 780                 
Ala Ile Gln Asp Leu Gly Ser Asp Ile Leu Lys Glu His Pro Val Glu 
785                 790                 795                 800 
Asn Asn Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Arg Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Val Ser Ser Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Asn Asp Val Val Lys Arg Met Lys Ser Phe 
865                 870                 875                 880 
Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Ala Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Arg Glu Phe Asp Glu Asn 
    930                 935                 940                 
Asn Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Asn Phe Arg Lys Glu Phe Glu Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Lys Ala Leu Leu Thr Arg Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Pro Lys Tyr Asn Tyr Arg Glu Arg Lys Ser Ala Thr Glu 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Met Phe Lys Thr Thr Ile 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Ala Trp Asp Lys Thr Lys His 
                1045                1050                1055    
Phe Ala Thr Val Lys Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Ala Arg Lys Asn Tyr Leu Asn 
    1090                1095                1100                
Thr Gln Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Phe Val Ala Asp Ile Glu Lys Gly Lys Ser Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Met Ile Gly Ile Thr Ile Met Glu Arg Ser Arg Phe Glu 
            1140                1145                1150        
Ser Asn Ser Val Ala Phe Leu Glu Glu Lys Gly Tyr Gln Asn Ile Arg 
        1155                1160                1165            
Glu Asn Thr Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Gln His Leu Val Lys Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Ile Asn Ser Leu Asn Lys Pro Glu His Ile Asn Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Thr Asp Leu Leu His His Ile Lys Asp Phe 
        1235                1240                1245            
Ser Glu Lys Asn Ile Leu Ala Ala Lys Asn Leu Glu Lys Ile Asn Glu 
    1250                1255                1260                
Ala Phe Glu Glu Asn Glu Glu Asp Ser Val Ser Glu Leu Ala Gln Ser 
1265                1270                1275                1280
Phe Ile Ser Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Val Leu Ser Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Glu 
    1330                1335                1340

<210> 58
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #113

<400> 58
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Arg Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys His Pro Ile Phe Gly Thr Leu Ala Glu Glu Lys Ala Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Asn Asp Val Gln Lys Leu Phe Asn Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Lys Thr Phe Glu Gly Ser His Leu Ser Glu Gln Asn Ala Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Ile Leu Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Phe Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Ser Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Gln Asn Leu Pro Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Met Arg Asp Tyr Phe Leu Asp Ser Gly Gln Lys 
    530                 535                 540                 
Lys Glu Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val Thr Glu 
545                 550                 555                 560 
Lys Asp Ile Ile Asp Tyr Leu Glu Asn Glu Phe Asp Glu Tyr Asp Gly 
                565                 570                 575     
Val Glu Leu Lys Gly Leu Glu Lys Gln Phe Asn Ala Ser Leu Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Glu Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Glu Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Thr Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Thr Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asp Leu Lys 
705                 710                 715                 720 
Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Tyr Lys Arg Leu Glu Asp 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Gln Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Arg Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Glu Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Ala Arg Phe Asn Thr Glu Leu Asp Glu Asn 
    930                 935                 940                 
Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Pro Lys Tyr Asn Tyr Arg Glu Arg Lys Lys Ala Thr Glu 
    1010                1015                1020                
Lys Val Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Lys Glu Val 
1025                1030                1035                1040
Lys Tyr Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Leu Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Ile Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Ile Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Thr Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Lys Lys Gly Tyr Lys Asn Ile Gln 
        1155                1160                1165            
Glu Asp Asn Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile His Lys Ile Asn Glu Pro Ile His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Lys Asn Glu Phe Lys Glu Leu Leu Asn Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Asn Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Leu Tyr Asp Gln Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Leu Gly Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Ile Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 59
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #114

<400> 59
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Arg Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Thr Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys Tyr Pro Ile Phe Gly Thr Leu Ala Glu Glu Lys Ala Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Phe Asn Ser 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Asp Phe Val Glu Ile Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Gly Ser His Leu Ser Glu Gln Asn Ala Gln Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 
Ile Leu Lys Leu Phe Pro Asp Glu Lys Asn Asn Gly Leu Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     
Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Ser Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Met Arg Asp Tyr Phe Leu Asp Ser Gly Gln Lys 
    530                 535                 540                 
Lys Glu Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val Thr Glu 
545                 550                 555                 560 
Lys Asp Ile Ile Asp Tyr Leu His Asn Glu Val Asp Gly Tyr Asp Gly 
                565                 570                 575     
Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Leu Asp Asp 
        595                 600                 605             
Ala Glu Asn Glu Ala Ile Leu Glu Asp Ile Val His Thr Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Lys Tyr Asp Asn 
625                 630                 635                 640 
Leu Phe Asp Glu Lys Val Leu Lys Lys Leu Thr Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Thr Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Thr Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asp Val Lys 
705                 710                 715                 720 
Gln Val Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Gln Gly Lys Arg Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu Asp 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu Asn Pro Thr Asp 
785                 790                 795                 800 
Asn Asn Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Gln Leu Ser 
            820                 825                 830         
Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Asp Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Gln Lys Arg Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Glu Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Ala Arg Phe Asn Thr Glu Leu Asp Glu Asn 
    930                 935                 940                 
Asp Lys Lys Ile Arg Thr Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Pro Lys Tyr Asn Tyr Arg Glu Arg Lys Lys Ala Thr Glu 
    1010                1015                1020                
Lys Val Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Lys Glu Val 
1025                1030                1035                1040
His Tyr Ala Asp Glu Thr Gly Glu Ile Val Trp Asn Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Leu Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Ile Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Ile Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Thr Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Thr Phe Leu Glu Glu Lys Gly Tyr Lys Asn Ile Gln 
        1155                1160                1165            
Glu Glu Asn Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Ile Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile His Lys Ile Asn Glu Pro Ile His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Lys Asn Glu Phe Lys Glu Leu Leu Asn Tyr Ile Ser Glu Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Asn Asn Leu Glu Lys Ile Lys Ser 
    1250                1255                1260                
Leu Tyr Asp Gln Asn Glu Asp Ala Asp Ile Asp Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Leu Gly Val Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Ser 
            1300                1305                1310        
Glu Ile Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 60
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #115

<400> 60
Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Ile Thr Ala Glu Gly Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala Thr Leu Asp Asp Ala 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Pro Asp Asp Lys Arg 
            100                 105                 110         
Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val Glu Glu Lys Ala Tyr 
        115                 120                 125             
His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 
Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Phe Asn Ser 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp Phe Leu Asp Thr Tyr 
            180                 185                 190         
Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu Asn Ser Lys Gln Leu 
        195                 200                 205             
Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp Arg 
    210                 215                 220                 
Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser Gly Ile Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys Tyr Phe 
                245                 250                 255     
Asn Leu Asp Glu Lys Ala Pro Leu His Phe Ser Lys Glu Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly Asp Asp Tyr Ser Asp 
        275                 280                 285             
Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Gly Thr Glu Ala Pro Leu Ser Ser Ala 
305                 310                 315                 320 
Met Ile Met Arg Tyr Lys Glu His Glu Glu Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Ala Tyr Ile Arg Asn Ile Ser Leu Glu Thr Tyr Asn Glu Val Phe Lys 
            340                 345                 350         
Asp Asn Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Val Tyr Leu Lys Asn Leu Leu Ser Asn Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala Lys Phe Tyr Pro Phe 
            420                 425                 430         
Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro Trp Asn Phe Glu Asp 
465                 470                 475                 480 
Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Phe Ile Ala Glu Ser Met Arg Asp Tyr Phe Leu Asp Ser Lys Gln Lys 
    530                 535                 540                 
Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp Lys Arg Lys Val Thr Asp 
545                 550                 555                 560 
Lys Asp Ile Ile Glu Tyr Leu His Ala Glu Ile Asp Gly Tyr Asp Gly 
                565                 570                 575     
Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ser Ser Leu Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys Glu Phe Leu Asp Asp 
        595                 600                 605             
Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile His Thr Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ser Lys Phe Glu Asn 
625                 630                 635                 640 
Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Glu 
            660                 665                 670         
Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ile Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Lys Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp Lys Asp Asn Ile Lys 
705                 710                 715                 720 
Gln Val Val Gln Ala Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Arg 
            740                 745                 750         
Lys Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln Tyr Thr 
        755                 760                 765             
Asn Gln Gly Lys Ser Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ser Leu Lys Glu Leu Gly Ser Lys Ile Leu Lys Glu Asn Ile Ile Asp 
785                 790                 795                 800 
Asn Asn Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Arg Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Leu Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Val Ser Ser Ala Ser Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Leu Glu Val Val Lys Lys Arg Lys Thr Leu 
865                 870                 875                 880 
Trp Tyr Gln Leu Leu Lys Ser Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Arg Glu Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Leu Leu Asp Glu Arg Phe Asn Asn Lys Lys Asp Glu Asn 
    930                 935                 940                 
Asn Lys Thr Val Arg Thr Val Lys Ile Ile Thr Leu Lys Ser Ser Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Asp Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Phe His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Ser Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Pro Lys Tyr Asn Phe Arg Glu Arg Lys Ser Ala Thr Glu 
    1010                1015                1020                
Lys Val Tyr Phe Tyr Ser Asn Ile Met Asn Ile Phe Lys Lys Ser Ile 
1025                1030                1035                1040
Ser Leu Ala Asp Glu Thr Gly Glu Ser Val Trp Asn Lys Glu Ser Asp 
                1045                1050                1055    
Leu Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Asn His Gly Leu Asp Arg Lys Gly Leu Phe 
        1075                1080                1085            
Asn Ala Asn Leu Ser Ser Lys Pro Lys Pro Asn Ser Glu Tyr Leu Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Tyr Ala Gly Ile Ser Asn Ser Phe Ala Val 
1105                1110                1115                1120
Leu Val Lys Gly Thr Ile Glu Lys Gly Ala Lys Lys Lys Ile Thr Asn 
                1125                1130                1135    
Val Leu Glu Phe Gln Gly Ile Ser Ile Leu Asp Arg Ile Thr Tyr Arg 
            1140                1145                1150        
Lys Asp Lys Leu Asn Phe Leu Leu Glu Lys Gly Tyr Lys Asp Ile Glu 
        1155                1160                1165            
Glu Glu Leu Ile Ile Glu Leu Pro Lys Tyr Ser Leu Phe Glu Leu Ser 
    1170                1175                1180                
Asp Gly Ser Arg Arg Met Leu Ala Ser Ile Gly Glu Ile His Lys Gly 
1185                1190                1195                1200
Asn Gln Ile Phe Leu Ser Gln Lys Phe Val Lys Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Ser Asn Thr Ile Asn Glu Asn His Arg Lys Tyr Val Glu 
            1220                1225                1230        
Asn His Lys Lys Glu Phe Glu Glu Leu Phe Tyr Tyr Ile Leu Glu Phe 
        1235                1240                1245            
Asn Glu Asn Tyr Val Gly Ala Lys Lys Asn Gly Glu Leu Leu Lys Ser 
    1250                1255                1260                
Ala Phe Gln Ser Trp Gln Asn His Ser Ile Asp Glu Leu Cys Ser Ser 
1265                1270                1275                1280
Phe Ile Gly Leu Phe Glu Leu Thr Ser Arg Gly Ser Ala Ala Asp Phe 
                1285                1290                1295    
Glu Phe Leu Gly Val Lys Ile Pro Arg Tyr Arg Tyr Thr Pro Ser Ser 
            1300                1305                1310        
Leu Leu Lys Asp Ala Thr Leu Ile His Gln Ser Val Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ala Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 61
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #116

<400> 61
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Arg Phe Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Ser Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ala Phe Asp Ile 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln Val 
        195                 200                 205             
Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     
Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Asp Glu Val Phe Ser 
            340                 345                 350         
Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Asn Ser Asp Glu Ala Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Ile Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Leu Arg Asp Tyr Phe Leu Asp Ser Gly Gln Lys 
    530                 535                 540                 
Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val Thr Glu 
545                 550                 555                 560 
Lys Asp Ile Ile His Tyr Leu His Asn Glu Val Asp Gly Tyr Asp Gly 
                565                 570                 575     
Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp Asp 
        595                 600                 605             
Ala Lys Asn Glu Ala Ile Leu Glu Asn Ile Val His Thr Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp Ser 
625                 630                 635                 640 
Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Thr Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Asn Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe Lys 
    690                 695                 700                 
Glu Ile Ile Gln Lys Ala Gln Val Ile Gly Lys Thr Asp Asp Val Lys 
705                 710                 715                 720 
Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu Asp 
    770                 775                 780                 
Ala Leu Lys Asn Leu Ala Ser Gly Ile Leu Lys Glu Asn Pro Thr Asp 
785                 790                 795                 800 
Asn Asn Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Ala Leu Asp Ile Asn Gln Leu Ser 
            820                 825                 830         
Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys Asp Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Gln Lys Arg Lys Ala Phe 
865                 870                 875                 880 
Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Glu Arg Lys Phe Asn 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp Glu Arg Asp Lys Val 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Val Asn Glu Lys 
    930                 935                 940                 
Asp Lys Lys Asn Arg Thr Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Asn Phe Arg Lys Glu Phe Arg Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Lys Ala Ile Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Gln Lys Tyr Asp Pro Lys Glu Val Glu Lys Ala Thr Glu 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Leu Asn Phe Phe Lys Glu Glu Val 
1025                1030                1035                1040
His Tyr Ala Asp Asp Thr Gly Glu Ile Ala Trp Asn Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Ile Lys Lys Val Leu Ser Leu Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Ile Ser Leu Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Ile Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Ile Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Lys Ala Ala Phe Glu 
            1140                1145                1150        
Glu Asn Pro Ile Thr Phe Leu Glu Glu Lys Gly Tyr Lys Asn Ile Arg 
        1155                1160                1165            
Lys Glu Asn Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Ile Val Leu Pro Val His Leu Thr Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile His Lys Ile Asn Glu Pro Ile His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Lys Glu Leu Leu Asn Leu Ile Ser Glu Phe 
        1235                1240                1245            
Ser Gln Lys Tyr Ile Leu Ala Asp Asn Asn Leu Glu Lys Ile Lys Ser 
    1250                1255                1260                
Leu Tyr Asp Gln Asn Glu Asp Ala Asp Ile Asp Gln Leu Ala Asn Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Leu Gly Val Asp Ile Asp Arg Lys Arg Tyr Thr Ser Val Ser 
            1300                1305                1310        
Glu Ile Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 62
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - marginal ancestral sequence reconstruction method
      – node #117

<400> 62
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Ser Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu Tyr 
        115                 120                 125             
His Lys Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp Ile 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln Val 
        195                 200                 205             
Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 
Val Leu Lys Leu Phe Ser Asp Glu Lys Ser Thr Gly Leu Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     
Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala Asp 
        275                 280                 285             
Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Asp Glu Val Phe Ser 
            340                 345                 350         
Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Ile Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Leu Arg Asp Tyr Phe Leu Asp Ser Gly Gln Lys 
    530                 535                 540                 
Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val Thr Glu 
545                 550                 555                 560 
Lys Asp Ile Ile His Tyr Leu His Asn Glu Val Asp Gly Tyr Asp Gly 
                565                 570                 575     
Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp Asp 
        595                 600                 605             
Ala Lys Asn Glu Ala Ile Leu Glu Asn Ile Ile His Thr Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp Ser 
625                 630                 635                 640 
Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Thr Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Asp Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe Lys 
    690                 695                 700                 
Glu Ile Ile Gln Lys Ala Gln Val Val Gly Lys Thr Asp Asp Val Lys 
705                 710                 715                 720 
Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu Asp 
    770                 775                 780                 
Ala Leu Lys Asn Leu Ala Pro Gly Ile Leu Lys Glu Asn Pro Thr Asp 
785                 790                 795                 800 
Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Lys Ala Leu Asp Ile Asn Gln Leu Ser 
            820                 825                 830         
Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys Asp Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Gln Lys Arg Lys Ala Phe 
865                 870                 875                 880 
Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Glu Arg Lys Phe Asn 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp Glu Arg Asp Lys Val 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Val Asn Glu Lys 
    930                 935                 940                 
Asp Lys Lys Asn Arg Thr Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Asn Phe Arg Lys Glu Phe Lys Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Lys Ala Ile Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Gln Lys Tyr Asp Pro Lys Asp Val Glu Lys Ala Thr Glu 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Leu Asn Phe Phe Lys Glu Glu Val 
1025                1030                1035                1040
His Tyr Ala Asp Asp Thr Gly Glu Ile Ala Trp Asn Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Ile Lys Lys Val Leu Ser Leu Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Glu Gln Thr Gly Gly Leu Phe Asp Asn Asn Ile Val 
        1075                1080                1085            
Ser Lys Lys Asp Ala Ser Lys Leu Ile Pro Ile Lys Ser Ser Leu Ser 
    1090                1095                1100                
Pro Glu Lys Tyr Gly Gly Tyr Ala Arg Pro Thr Ile Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Ile Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Arg 
                1125                1130                1135    
Ile Lys Glu Met Val Gly Ile Thr Ile Gln Asp Lys Lys Lys Phe Glu 
            1140                1145                1150        
Ala Asn Pro Ile Thr Tyr Leu Glu Glu Arg Gly Tyr Lys Asn Ile Asn 
        1155                1160                1165            
Ser Asn Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Phe Asn 
    1170                1175                1180                
Asp Gly Gln Arg Arg Leu Leu Ala Ser Ser Ile Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Leu Ile Leu Pro Tyr His Leu Thr Ala Leu Leu Tyr His Ala 
                1205                1210                1215    
Gln Arg Ile Asn Lys Ile Asn Glu Pro Ile His Lys Gln Tyr Val Glu 
            1220                1225                1230        
Thr His Gln Asn Glu Phe Lys Glu Leu Leu Thr Thr Ile Ile Ser Leu 
        1235                1240                1245            
Ser Lys Lys Tyr Ile Gln Lys Pro Asn Val Glu Ser Leu Leu Gln Gln 
    1250                1255                1260                
Ala Phe Asp Gln Asn Ala Asp Lys Asp Ile Tyr Gln Leu Ser Glu Ser 
1265                1270                1275                1280
Phe Ile Ser Leu Leu Lys Leu Thr Ser Phe Gly Ala Pro Gly Ala Phe 
                1285                1290                1295    
Lys Phe Leu Gly Val Glu Ile Ser Gln Ser Arg Tyr Lys Ser Val Ser 
            1300                1305                1310        
Gln Phe Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 63
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #60

<400> 63
Met Lys Lys Glu Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Lys Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Leu Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Ser Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
His Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Trp Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Gln Glu Glu Ile Ser Lys Val Asp Pro Ser 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Asp Asp Arg His Pro Leu Phe Gly Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Met Glu 
    130                 135                 140                 
Asn Asp Glu Lys His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Ile Glu Gly Asp Leu Ser Ser 
                165                 170                 175     
Lys Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Leu Val Gln Val Phe 
            180                 185                 190         
Asn Asp Ala Leu Glu Glu Glu His Leu Ser Glu Glu Ala Asn Asp Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Arg Ser Ala Arg Ala Lys Glu 
    210                 215                 220                 
Val Val Lys Leu Phe Ala Ser Glu Lys Lys Gln Ala Cys Ile Ser Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Val Gly Leu Lys Gly Asn Leu Lys Lys Val Phe 
                245                 250                 255     
Gly Asp Ser Glu Asp Thr Ala Ile His Phe Ser Lys Asp Asn Tyr Glu 
            260                 265                 270         
Glu Asp Val Glu Ala Ile Arg Asp Thr Ile Pro Asp Glu Tyr Ser Glu 
        275                 280                 285             
Leu Phe Glu Ala Ile Lys Ala Leu Tyr Asp Ala Ile Val Leu Ser Gly 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Asn Asn Thr Glu Ala Lys Val Ser Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Gln His Arg Lys Asp Leu Lys Leu Leu Lys 
                325                 330                 335     
Asn Phe Val Lys Thr Tyr Leu Pro Glu Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Asp Thr Lys Asn Gly Tyr Ser Ala Tyr Ile Lys Gly Gly Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Asn Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Thr Ser Asp Asn Gly Val Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Ala Glu Lys Ile Glu Ser Ile Leu Ala Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Gly Asn Gly Arg Phe Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Glu Asp Gly Val Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Gly Asn Cys Thr Tyr Leu Pro Gly Glu Thr Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Leu Asn Glu Leu Asn Asn Val Arg 
        515                 520                 525             
Leu Thr Ile Asp Lys Gly Lys Trp Arg Arg Phe Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Arg Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Glu Asp Tyr Leu Lys Arg Glu Tyr Gly Glu Phe Asp Ser 
                565                 570                 575     
Ala Asp Ile Ser Gly Ile Asp Gly Glu Phe Lys Ser Ser Leu Gly Ser 
            580                 585                 590         
Tyr His Asp Phe Cys Lys Ile Leu Val Lys Ala Asp Ser Leu Asp Glu 
        595                 600                 605             
Glu Asp Tyr Lys Asp Ile Ile Glu Glu Ile Ile Lys Trp Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Leu Lys Arg Arg Leu Glu Lys Tyr Gly Glu 
625                 630                 635                 640 
Ile Leu Thr Glu Glu Gln Ile Lys Lys Ile Cys Lys Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Val Arg Asp Ala 
            660                 665                 670         
Glu Thr Gly Lys Ser Ile Ile Asp Val Leu Arg Glu Thr Asp Pro Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Leu Ser Asp Glu Asp Leu Gly Phe Gln 
    690                 695                 700                 
Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Glu Asn Leu Lys 
705                 710                 715                 720 
Glu Ile Val Glu Asp Leu Pro Gly Ser Pro Ala Val Lys Arg Gly Ile 
                725                 730                 735     
Asn Gln Ala Leu Arg Ile Val Asp Glu Ile Val Lys Val Met Gly His 
            740                 745                 750         
Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Glu Lys Gln Gln Ser 
        755                 760                 765             
Lys Arg Gly Arg Arg Ser Thr Lys Lys Arg Leu Lys Arg Leu Gln Glu 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Thr Asn Leu Trp Lys Glu Leu Leu Arg Asp 
785                 790                 795                 800 
Asp Asn Glu Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Thr Gln Met 
                805                 810                 815     
Gly Lys Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Asp Asp Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Val Ser Arg Glu Glu Asn Ala Arg Lys 
    850                 855                 860                 
Thr Asp Asn Leu Pro Ser Pro Glu Ile Arg Arg Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Met Leu Leu Asn Ala Gly Leu Ile Ser Lys Lys Lys Phe Asp 
                885                 890                 895     
Arg Leu Thr Arg Thr Asp Arg Gly Glu Phe Thr Glu Asp Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Thr Leu Leu Lys Gln Arg Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Lys Asn Thr Lys Ile Val Ser Val Lys Ala Asn Leu 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Gly Leu Ile Lys Cys Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Asn Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Ala Glu Phe Ile Lys 
        995                 1000                1005            
Gly Asp Tyr Arg Arg Asn Asn Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Met His Phe Tyr Gly Asn Ile Met Asn Ser Phe Thr Ser Asp Glu 
1025                1030                1035                1040
Pro Ile Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Ile Ala Thr Val Arg Lys Val Met Asn Tyr His Gln Val Leu Ile Thr 
            1060                1065                1070        
Arg Lys Val Glu Glu Glu Thr Gly Gly Phe Phe Asp Gln Thr Ile Tyr 
        1075                1080                1085            
Ser Lys Gly Asn Ser Lys Lys Leu Ile Pro Leu Lys Lys Asn Leu Asp 
    1090                1095                1100                
Pro Glu Lys Tyr Gly Gly Tyr Asn Ser Glu Thr Ala Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Glu Tyr Asp Ile Glu Lys Gly Lys Ala Lys Lys Glu Lys Thr 
                1125                1130                1135    
Val Lys Gln Leu Val Pro Ile Pro Val Arg Glu Lys Ala Ala Leu Glu 
            1140                1145                1150        
Lys Asn Pro Ser Lys Tyr Leu Glu Lys Lys Gly Tyr Glu Asn Pro Lys 
        1155                1160                1165            
Val Asn Leu Leu Ile Lys Ile Pro Lys Asn Gln Leu Leu Glu Leu Asp 
    1170                1175                1180                
Gly Gly Arg Arg Arg Ile Leu Ala Ala Ala Lys Glu Leu Lys Asn Ala 
1185                1190                1195                1200
Thr Gln Leu Val Leu Pro Ala Glu Glu Tyr Thr Leu Leu Glu Lys Val 
                1205                1210                1215    
Ala Lys Ile Ala Glu Lys Asn Asn Ser Glu Ser Ile Gly Tyr Val Glu 
            1220                1225                1230        
Glu Leu Leu Ala Gln Phe Asp Glu Leu Leu Asp Ser Leu Ile Asp Tyr 
        1235                1240                1245            
Ser Pro Lys Leu Ala Leu Gln Asp Lys Thr Leu Glu Lys Leu Lys Glu 
    1250                1255                1260                
Ala Phe Glu Lys Leu Asn Leu Ala Asp Lys Lys Glu Val Ala Lys Glu 
1265                1270                1275                1280
Ile Ile Gly Leu Leu His Cys Thr Ala Thr Thr Ala Asn Thr Ala Leu 
                1285                1290                1295    
Lys Ser Leu Gly Gly Ser Lys Asn Arg Met Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Lys Leu Asn Asn Ala Ser Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 64
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #61

<400> 64
Met Lys Lys Glu Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Lys Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Leu Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Ser Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
His Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Trp Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Gln Glu Glu Ile Ser Lys Val Asp Pro Ser 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Asp Asp Arg His Pro Leu Phe Gly Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Met Glu 
    130                 135                 140                 
Asn Asp Glu Lys His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Ile Glu Gly Asp Leu Ser Ser 
                165                 170                 175     
Lys Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Leu Val Gln Val Phe 
            180                 185                 190         
Asn Asp Ala Leu Glu Glu Glu His Leu Ser Glu Glu Ala Asn Asp Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Arg Ser Ala Arg Ala Lys Glu 
    210                 215                 220                 
Val Val Lys Leu Phe Ala Ser Glu Lys Lys Gln Ala Cys Ile Ser Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Val Gly Leu Lys Gly Asn Leu Lys Lys Val Phe 
                245                 250                 255     
Gly Asp Ser Glu Asp Thr Ala Ile His Phe Ser Lys Asp Asn Tyr Glu 
            260                 265                 270         
Glu Asp Val Glu Ala Ile Arg Asp Thr Ile Pro Asp Glu Tyr Ser Glu 
        275                 280                 285             
Leu Phe Glu Ala Ile Lys Ala Leu Tyr Asp Ala Ile Val Leu Ser Gly 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Asn Asn Thr Glu Ala Lys Val Ser Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Gln His Arg Lys Asp Leu Lys Leu Leu Lys 
                325                 330                 335     
Asn Phe Val Lys Thr Tyr Leu Pro Glu Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Asp Thr Lys Asn Gly Tyr Ser Ala Tyr Ile Lys Gly Gly Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Asn Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Thr Ser Asp Asn Gly Val Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Ala Glu Lys Ile Glu Ser Ile Leu Ala Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Gly Asn Gly Arg Phe Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Glu Asp Gly Val Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Gly Asn Cys Thr Tyr Leu Pro Gly Glu Thr Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Leu Asn Glu Leu Asn Asn Val Arg 
        515                 520                 525             
Leu Thr Ile Asp Lys Gly Lys Trp Arg Arg Phe Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Arg Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Glu Asp Tyr Leu Lys Arg Glu Tyr Gly Glu Phe Asp Ser 
                565                 570                 575     
Ala Asp Ile Ser Gly Ile Asp Gly Glu Phe Lys Ser Ser Leu Gly Ser 
            580                 585                 590         
Tyr His Asp Phe Cys Lys Ile Leu Val Lys Ala Asp Ser Leu Asp Glu 
        595                 600                 605             
Glu Asp Tyr Lys Asp Ile Ile Glu Glu Ile Ile Lys Trp Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Leu Lys Arg Arg Leu Glu Lys Tyr Gly Glu 
625                 630                 635                 640 
Ile Leu Thr Glu Glu Gln Ile Lys Lys Ile Cys Lys Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Val Arg Asp Ala 
            660                 665                 670         
Glu Thr Gly Lys Ser Ile Ile Asp Val Leu Arg Glu Thr Asp Pro Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Leu Ser Asp Glu Asp Leu Gly Phe Gln 
    690                 695                 700                 
Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Glu Asn Leu Lys 
705                 710                 715                 720 
Glu Ile Val Glu Asp Leu Pro Gly Ser Pro Ala Val Lys Arg Gly Ile 
                725                 730                 735     
Asn Gln Ala Leu Arg Ile Val Asp Glu Ile Val Lys Val Met Gly His 
            740                 745                 750         
Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Glu Lys Gln Gln Ser 
        755                 760                 765             
Lys Arg Gly Arg Arg Ser Thr Lys Lys Arg Leu Lys Arg Leu Gln Glu 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Thr Asn Leu Trp Lys Glu Leu Leu Arg Asp 
785                 790                 795                 800 
Asp Asn Glu Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Thr Gln Met 
                805                 810                 815     
Gly Lys Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Asp Asp Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Val Ser Arg Glu Glu Asn Ala Arg Lys 
    850                 855                 860                 
Thr Asp Asn Leu Pro Ser Pro Glu Ile Arg Arg Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Met Leu Leu Asn Ala Gly Leu Ile Ser Lys Lys Lys Phe Asp 
                885                 890                 895     
Arg Leu Thr Arg Thr Asp Arg Gly Glu Phe Thr Glu Asp Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Thr Leu Leu Lys Gln Arg Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Lys Asn Thr Lys Ile Val Ser Val Lys Ala Asn Leu 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Gly Leu Ile Lys Cys Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Asn Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Ala Glu Phe Ile Lys 
        995                 1000                1005            
Gly Asp Tyr Arg Arg Asn Asn Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Met His Phe Tyr Gly Asn Ile Met Asn Ser Phe Thr Ser Asp Glu 
1025                1030                1035                1040
Pro Ile Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Ile Ala Thr Val Arg Lys Val Met Asn Tyr His Gln Val Leu Ile Thr 
            1060                1065                1070        
Arg Lys Val Glu Glu Glu Thr Gly Gly Phe Phe Asp Gln Thr Ile Tyr 
        1075                1080                1085            
Ser Lys Gly Asn Ser Lys Lys Leu Ile Pro Leu Lys Lys Asn Leu Asp 
    1090                1095                1100                
Pro Glu Lys Tyr Gly Gly Tyr Asn Ser Glu Thr Ala Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Glu Tyr Asp Ile Glu Lys Gly Lys Ala Lys Lys Glu Lys Thr 
                1125                1130                1135    
Val Lys Gln Leu Val Pro Ile Pro Val Arg Glu Lys Ala Ala Leu Glu 
            1140                1145                1150        
Lys Asn Pro Ser Lys Tyr Leu Glu Lys Lys Gly Tyr Glu Asn Pro Lys 
        1155                1160                1165            
Val Asn Leu Leu Ile Lys Ile Pro Lys Asn Gln Leu Leu Glu Leu Asp 
    1170                1175                1180                
Gly Gly Arg Arg Arg Ile Leu Ala Ala Ala Lys Glu Leu Lys Asn Ala 
1185                1190                1195                1200
Thr Gln Leu Val Leu Pro Ala Glu Glu Tyr Thr Leu Leu Glu Lys Val 
                1205                1210                1215    
Ala Lys Ile Ala Glu Lys Asn Asn Ser Glu Ser Ile Gly Tyr Val Glu 
            1220                1225                1230        
Glu Leu Leu Ala Gln Phe Asp Glu Leu Leu Asp Ser Leu Ile Asp Tyr 
        1235                1240                1245            
Ser Pro Lys Leu Ala Leu Gln Asp Lys Thr Leu Glu Lys Leu Lys Glu 
    1250                1255                1260                
Ala Phe Glu Lys Leu Asn Leu Ala Asp Lys Lys Glu Val Ala Lys Glu 
1265                1270                1275                1280
Ile Ile Gly Leu Leu His Cys Thr Ala Thr Thr Ala Asn Thr Ala Leu 
                1285                1290                1295    
Lys Ser Leu Gly Gly Ser Lys Asn Arg Met Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Lys Leu Asn Asn Ala Ser Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 65
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #62

<400> 65
Thr Thr Asp Glu Tyr Ser Ile Gly Leu Asp Met Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Lys Asn Gly Ser Met Ala His Lys Lys Met 
            20                  25                  30          
Pro Thr Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Pro Thr Leu 
        35                  40                  45              
Gly Ser Arg Leu Phe Asp Ala Ala Glu Thr Ala Ala Glu Ala Arg Ala 
    50                  55                  60                  
His Arg Gly Gln Arg Arg Arg Tyr Val Arg Arg Arg Trp Arg Leu Asp 
65                  70                  75                  80  
Leu Leu Gln Gly Leu Phe Gln Asp Glu Ile Asn Lys Val Asp Ser Glu 
                85                  90                  95      
Phe Phe Ile Arg Leu Arg Gln Ser Arg Leu His Val Glu Asp Arg Ala 
            100                 105                 110         
Asp Tyr Arg Trp Pro Leu Phe Asn Asp Thr Asp Thr Glu Lys Asp Tyr 
        115                 120                 125             
Tyr Lys Arg Phe Pro Thr Ile Tyr His Leu Arg Lys Trp Leu Met Glu 
    130                 135                 140                 
Thr Asp Glu Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Val His Asn 
145                 150                 155                 160 
Ile Val Lys His Arg Gly Asn Phe Leu Arg Glu Lys Asn Leu Ser Ser 
                165                 170                 175     
Arg Asn Ala Asp Pro Ala Glu Ala Val Arg His Leu Arg Gln Ala Leu 
            180                 185                 190         
Asn Asp Trp Leu Glu Arg Gly Tyr Leu Ala Glu Ala Asp Asn His Ile 
        195                 200                 205             
Leu Asp Ile Leu Thr Asp Ser Lys Arg Arg Ser Asp Gln Ala Lys Glu 
    210                 215                 220                 
Ile Ala Lys Leu Phe Gly Thr Glu Gln Ser Lys Ala Cys Asn Ser Ala 
225                 230                 235                 240 
Leu Ala Lys Ala Ile Val Gly Leu Lys Ala Glu Leu Lys Asn Val Phe 
                245                 250                 255     
Gly Glu Ala Glu Lys Thr Ala Ile His Leu Ser Ser Asp Asn Tyr Glu 
            260                 265                 270         
Asp Asp Val Glu Ala Ile Arg Asp Ala Ile Pro Asp Asp Ser Gly Glu 
        275                 280                 285             
Leu Phe Gln Ala Leu Cys Ala Leu Tyr Ser Ala Tyr Val Leu Gln Gly 
    290                 295                 300                 
Leu Leu Ser Tyr Ala Pro Gly Gln Thr Glu Ala Lys Ile Ser Ala Asn 
305                 310                 315                 320 
Met Val Ala Lys Tyr Asp Gln Tyr Gly Lys Asp Leu Lys Leu Leu Lys 
                325                 330                 335     
Asn Leu Ala Arg Thr Tyr Val Ser Glu Lys Tyr Asp Ala Phe Phe Arg 
            340                 345                 350         
Gly Glu Thr Tyr Glu Gly Ser Ser Asp Tyr Val Gln Gly Tyr Ala Ser 
        355                 360                 365             
Tyr Asp Asp Phe Gln Lys Ser Val Lys Lys Leu Phe Lys Gln Leu Glu 
    370                 375                 380                 
Glu Asp Arg Tyr Met Met Lys Ala Phe Gly Glu Glu Lys Phe Leu Arg 
385                 390                 395                 400 
Arg Leu Lys Thr Ser Asp Asn Gly Ser Ile Tyr Tyr Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys His Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Glu Glu Glu Lys Ile Glu Ser Leu Val Ser Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Glu Asn Glu Arg Phe Ala Trp 
    450                 455                 460                 
Ser Ser Arg Met Glu Asp Ala Val Ile Thr Pro Trp Asn Trp Asp Glu 
465                 470                 475                 480 
Ile Ile Asp Arg Asp Lys Ser Ala Glu Glu Phe Ile Leu Arg Met Thr 
                485                 490                 495     
Gly Asp Cys Thr Tyr Leu Gln Gly Glu Pro Val Leu Pro Lys Cys Ser 
            500                 505                 510         
Leu Leu Tyr Glu Glu Phe Cys Val Leu Asn Glu Leu Asn Gly Ala Arg 
        515                 520                 525             
Trp Thr Ile Asp Glu Asp Asp Trp Arg Arg Phe Asp Ala Ala Gln Arg 
    530                 535                 540                 
Glu Gly Ile Leu His Asp Leu Phe Arg Lys Thr Arg Lys Val Thr Tyr 
545                 550                 555                 560 
Lys Lys Val Ala Asp Trp Leu Glu Arg Glu Tyr Glu Asn Phe Ala Asn 
                565                 570                 575     
Ala His Val Arg Gly Gly Gln Gly Glu Phe Glu Ser Lys Leu Gly Ser 
            580                 585                 590         
Tyr Ile Phe Phe Cys Lys Asp Val Phe Lys Thr Asp Ser Leu Asp Glu 
        595                 600                 605             
Ala Asp Tyr Lys Pro Met Ile Glu Gln Ile Ile Leu Trp Ser Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Asp Ile Leu Lys Arg Lys Ile Glu Ala Tyr Gly Glu 
625                 630                 635                 640 
His Leu Thr Asp Glu Gln Ile Lys Val Ile Cys Lys Lys Arg Phe Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Ile Lys Asp Ala 
            660                 665                 670         
Asp Thr Gly Arg Ser Ile Ile Asp Val Leu Arg Glu Gly Asn Pro Asn 
        675                 680                 685             
Ser Arg Thr Phe Met Glu Val Leu Arg Asp Glu Asp Leu Gly Phe Gln 
    690                 695                 700                 
Lys Lys Ile Asp Asp Phe Asn Lys Asp Tyr Ala Gly Glu Asn Leu Met 
705                 710                 715                 720 
Asp Ile Val Asn Asp Leu Pro Gly Ser Pro Ala Ile Arg Arg Ser Ile 
                725                 730                 735     
Asn Gln Ala Ile Arg Ile Val Asp Glu Ile Val Ser Ile Ala Gly Lys 
            740                 745                 750         
Glu Pro Ser Asn Ile Phe Ile Glu Val Thr Arg Glu Glu Asp Asp Pro 
        755                 760                 765             
Arg Lys Lys Gly Arg Arg Thr Lys Arg Arg Tyr Glu Ala Ile Lys Asn 
    770                 775                 780                 
Ala Leu Glu Asn Phe Lys Thr Asp Leu Trp Lys Glu Leu Leu Lys Lys 
785                 790                 795                 800 
Asp Ser Asn Asn Leu Asp Glu Arg Leu Ser Leu Tyr Phe Met Gln Arg 
                805                 810                 815     
Gly Lys Cys Met Tyr Ser Gly Arg Pro Ile Lys Leu Glu Glu Leu His 
            820                 825                 830         
Lys Tyr Glu Val Asp His Ile Ile Pro Arg Ser Tyr Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Glu Asn Lys Val Leu Val Cys Arg Glu Glu Asn Gln Arg Lys 
    850                 855                 860                 
Thr Asp Thr Leu Leu Asp Pro Ala Ile Arg Arg Lys Met Gly Lys Tyr 
865                 870                 875                 880 
Trp Arg Met Leu His Asn Ala Lys Leu Ile Gly Asp Lys Lys Phe Asn 
                885                 890                 895     
Asn Leu Phe Arg Arg Asp Arg Gly Glu Ile Asp Asp Lys Thr Leu Lys 
            900                 905                 910         
Gly Phe Val Ala Arg Gln Leu Val Glu Thr Ser Gln Met Val Lys Leu 
        915                 920                 925             
Val Gln Thr Leu Leu Glu Ala Arg Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Lys Asn Thr Lys Ile Val Pro Val Lys Ala Asn Ile 
945                 950                 955                 960 
Ser His Asp Leu Arg Glu Ala Ala Gly Leu Val Lys Cys Arg Glu Ala 
                965                 970                 975     
Asn Asp Phe His His Ala His Asp Ala Tyr Leu Ala Cys Arg Val Gly 
            980                 985                 990         
Leu Phe Ile Gln Lys Arg His Pro Ser Val Tyr Ala Asn His Ile Met 
        995                 1000                1005            
Arg Asp Tyr Ala Arg Lys Gln Ala Arg Glu Tyr Asn Lys Thr His Ser 
    1010                1015                1020                
Met Ala His Thr Lys Gly Phe Ile Val Asn Ser Phe Met Ser Ser Glu 
1025                1030                1035                1040
Phe Asp Lys Asp Glu Thr Gly Glu Ile Phe Trp Asp Ala Glu Ala Glu 
                1045                1050                1055    
Val Glu Asp Ile Arg Arg Val Leu Asn Tyr Arg Gln Cys Phe Ile Ser 
            1060                1065                1070        
Arg Met Pro Val Glu Asp Thr Gly Ala Phe Trp Asp Gln Thr Ile Tyr 
        1075                1080                1085            
Ser Pro Arg Ser Asn Lys Lys Leu Leu Pro Leu Lys Arg Asp Leu Asp 
    1090                1095                1100                
Pro Asp Lys Tyr Gly Ser Tyr Ser Arg Glu Gln Phe Ala Tyr Ser Val 
1105                1110                1115                1120
Phe Phe Ile Tyr Asp Ile Glu Ala Arg Asp Ala Lys Lys Glu Lys Thr 
                1125                1130                1135    
Val Leu Gln Phe Ala Gln Val Pro Val Arg Val Thr Ala Ala Ile Ala 
            1140                1145                1150        
Lys Asp Pro Ser Lys Tyr Ala Ser Lys Leu Gly Gln Glu Asn Leu Glu 
        1155                1160                1165            
Phe Thr Arg Ile Ile Arg Ile Leu Lys Lys Gln Leu Leu Glu Leu Asp 
    1170                1175                1180                
Asn Gly Asp Arg Leu Ile Ile Thr Gly Ala Lys Glu Val Arg Asn Ala 
1185                1190                1195                1200
Thr Glu Phe Ala Phe Ser Leu Glu Glu Thr Thr Leu Val Glu Arg Cys 
                1205                1210                1215    
Ala Gln Ile Ala Lys Glu Gly Asn Pro Glu Asp Ile Gly Tyr Val Glu 
            1220                1225                1230        
Gln Leu Ser Ala Gln Leu Asn Glu Leu Phe Ser Ser Leu Ile Glu Arg 
        1235                1240                1245            
Gly Pro Lys Leu Gly Val Arg Leu His Ala Gln Leu Lys Leu Asp Gln 
    1250                1255                1260                
Tyr Leu Arg Lys Phe Asp Asp Ala Ala Leu Arg Asp Lys Ala Lys Val 
1265                1270                1275                1280
Ile Ile Gly Leu Ile Ser Ile Thr Asn Gly Ala Thr Asn Thr Ala Leu 
                1285                1290                1295    
Ser Ser Leu Gly Gly Ser Lys Asn Arg Met Arg Tyr Thr His Asn Lys 
            1300                1305                1310        
Leu Leu Asn Asn Ala Ser Leu Ile Asp Gln Ser Val Thr Gly Met Phe 
        1315                1320                1325            
Glu Arg Arg Thr His Ile Gly Leu Leu Gly Glu Asp 
    1330                1335                1340

<210> 66
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #63

<400> 66
Met Lys Lys Glu Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Lys Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Leu Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Ser Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
His Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Gln Glu Glu Ile Ser Lys Val Asp Pro Ser 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Asp Asp Arg His Pro Leu Phe Gly Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Met Glu 
    130                 135                 140                 
Asn Asp Glu Lys His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Ile Glu Gly Asp Leu Ser Ser 
                165                 170                 175     
Lys Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Leu Val Gln Val Phe 
            180                 185                 190         
Asn Asp Ala Leu Glu Glu Glu His Leu Ser Glu Glu Ala Ile Asp Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Arg Ser Ala Arg Ala Lys Glu 
    210                 215                 220                 
Val Val Lys Leu Phe Ala Ser Glu Lys Lys Gln Ala Cys Ile Ser Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Val Gly Leu Lys Gly Asn Leu Lys Lys Val Phe 
                245                 250                 255     
Gly Asp Ser Glu Asp Thr Ala Ile His Phe Ser Lys Asp Asn Tyr Glu 
            260                 265                 270         
Glu Asp Val Glu Ala Ile Arg Asp Thr Ile Pro Asp Glu Tyr Ser Glu 
        275                 280                 285             
Leu Phe Glu Ala Ile Lys Ala Leu Tyr Asp Ala Ile Val Leu Ser Gly 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Asn Asn Thr Glu Ala Lys Val Ser Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Gln His Arg Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Asn Phe Val Lys Thr Tyr Leu Pro Glu Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Asp Thr Lys Asn Gly Tyr Ser Ala Tyr Ile Lys Gly Gly Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Asn Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Thr Ser Asp Asn Gly Val Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Ser Ile Leu Ala Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Gly Asn Gly Arg Phe Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Glu Asp Gly Val Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Asn Cys Thr Tyr Leu Pro Gly Glu Thr Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Leu Asn Glu Leu Asn Asn Val Arg 
        515                 520                 525             
Leu Thr Ile Asp Lys Gly Lys Trp Arg Arg Phe Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Arg Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Glu Asp Tyr Leu Lys Arg Glu Tyr Gly Glu Phe Asp Ser 
                565                 570                 575     
Ala Asp Ile Ser Gly Ile Asp Gly Glu Phe Lys Ser Ser Leu Gly Ser 
            580                 585                 590         
Tyr His Asp Phe Cys Lys Ile Leu Val Lys Ala Asp Ser Leu Asp Glu 
        595                 600                 605             
Glu Asp Tyr Lys Asp Ile Ile Glu Glu Ile Ile Lys Trp Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Leu Lys Arg Arg Leu Glu Lys Tyr Ser Glu 
625                 630                 635                 640 
Ile Leu Thr Glu Glu Gln Ile Lys Lys Ile Cys Lys Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Val Arg Asp Lys 
            660                 665                 670         
Glu Thr Gly Lys Ser Ile Ile Asp Val Leu Arg Glu Thr Asp Pro Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Leu Ser Asp Glu Asp Leu Gly Phe Gln 
    690                 695                 700                 
Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Glu Asn Leu Lys 
705                 710                 715                 720 
Glu Ile Val Glu Asp Leu Pro Gly Ser Pro Ala Val Lys Arg Gly Ile 
                725                 730                 735     
Asn Gln Ala Leu Arg Ile Val Asp Glu Ile Val Lys Val Met Gly His 
            740                 745                 750         
Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Glu Lys Gln Gln Ser 
        755                 760                 765             
Lys Arg Gly Arg Arg Ser Thr Lys Lys Arg Leu Lys Arg Leu Gln Glu 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Thr Asn Leu Trp Lys Glu Leu Leu Arg Asp 
785                 790                 795                 800 
Asp Asn Glu Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Thr Gln Met 
                805                 810                 815     
Gly Lys Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Asp Asp Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Val Ser Arg Glu Glu Asn Ala Arg Lys 
    850                 855                 860                 
Thr Asp Asn Leu Pro Ser Pro Glu Ile Arg Arg Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Met Leu Leu Asn Ala Gly Leu Ile Ser Lys Lys Lys Phe Asp 
                885                 890                 895     
Arg Leu Thr Arg Thr Asp Arg Gly Glu Phe Thr Glu Asp Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Thr Leu Leu Lys Gln Arg Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Lys Asn Thr Lys Ile Val Ser Val Lys Ala Asn Leu 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Gly Leu Ile Lys Cys Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Asn Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Ala Glu Phe Ile Lys 
        995                 1000                1005            
Gly Asp Tyr Arg Arg Asn Asn Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Met His Phe Tyr Gly Asn Ile Met Asn Ser Phe Thr Ser Asp Glu 
1025                1030                1035                1040
Pro Ile Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Ile Ala Thr Val Arg Lys Val Met Asn Tyr His Gln Val Leu Ile Thr 
            1060                1065                1070        
Arg Lys Val Glu Glu Glu Thr Gly Gly Phe Phe Asp Gln Thr Ile Leu 
        1075                1080                1085            
Ser Lys Gly Asn Ser Lys Lys Leu Ile Pro Leu Lys Lys Asn Leu Asp 
    1090                1095                1100                
Pro Glu Lys Tyr Gly Gly Tyr Asn Ser Pro Thr Ala Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Glu Tyr Asp Ile Glu Lys Gly Lys Ala Lys Lys Glu Lys Thr 
                1125                1130                1135    
Val Lys Gln Leu Val Pro Ile Pro Val Arg Glu Lys Ala Ala Leu Glu 
            1140                1145                1150        
Lys Asn Pro Ile Lys Tyr Leu Glu Lys Lys Gly Tyr Glu Asn Pro Lys 
        1155                1160                1165            
Val Asn Leu Leu Ile Lys Ile Pro Lys Asn Ser Leu Leu Glu Leu Asp 
    1170                1175                1180                
Gly Gly Arg Arg Arg Ile Leu Ala Ala Ala Lys Glu Leu Lys Asn Ala 
1185                1190                1195                1200
Thr Gln Leu Val Leu Pro Ala Glu Glu Tyr Thr Leu Leu Glu Lys Val 
                1205                1210                1215    
Ala Lys Ile Ala Glu Lys Asn Asn Ser Glu Ser Ile Gly Tyr Val Glu 
            1220                1225                1230        
Glu Leu Leu Ala Gln Phe Asp Glu Leu Leu Asp Ser Leu Ile Asp Tyr 
        1235                1240                1245            
Ser Pro Lys Leu Ala Leu Gln Asp Lys Thr Leu Glu Lys Leu Lys Glu 
    1250                1255                1260                
Ala Phe Glu Lys Leu Asn Leu Ala Asp Lys Lys Glu Val Ala Lys Glu 
1265                1270                1275                1280
Ile Ile Gly Leu Leu His Cys Thr Ala Thr Thr Ala Asn Thr Ala Leu 
                1285                1290                1295    
Lys Ser Leu Gly Gly Ser Lys Asn Arg Met Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Lys Leu Asn Asn Ala Ser Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 67
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #64

<400> 67
Met Lys Lys Glu Tyr Tyr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Val Lys Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Thr Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Tyr Arg Thr Ser Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ser Glu Glu Ile Ser Lys Val Asp Pro Ser 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Glu Asp Arg Tyr Pro Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Met Glu 
    130                 135                 140                 
Asn Asp Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Ile Glu Gly Asp Leu Ser Ser 
                165                 170                 175     
Lys Asn Thr Asp Leu Gly Glu Val Phe Ser Gln Leu Val Lys Val Phe 
            180                 185                 190         
Asn Asp Ala Leu Glu Ile Glu Leu Leu Ser Glu Glu Ala Leu Glu Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Lys Ser Ala Lys Ala Lys Glu 
    210                 215                 220                 
Val Val Lys Leu Phe Ala Ser Glu Lys Lys Gln Ala Cys Ile Lys Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Val Gly Leu Thr Gly Asn Leu Ser Glu Ile Phe 
                245                 250                 255     
Gly Asp Ser Glu Asp Thr Ser Ile Asn Phe Ser Asp Ser Asn Tyr Glu 
            260                 265                 270         
Glu Asn Val Glu Ala Ile Glu Asp Thr Ile Pro Asp Glu Phe Ser Glu 
        275                 280                 285             
Leu Ile Glu Ala Ile Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Gly 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Thr Tyr Leu Pro Glu Glu Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Lys Asn Gly Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Leu Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Ser Ile Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Gly Asn Gly Ser Asn Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Glu Asp Gly Lys Ile Tyr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Val Val Asp Val Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Asp Lys Ile Lys Trp Arg Arg Phe Ser Val Glu Leu Lys 
    530                 535                 540                 
Gln Glu Ile Tyr Asn Asp Leu Phe Cys Arg Asn Arg Lys Val Thr Gly 
545                 550                 555                 560 
Lys Lys Leu Lys Asp Tyr Leu Lys Arg Glu Tyr Gly Glu Leu Asp Glu 
                565                 570                 575     
Ala Asp Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ser Leu Gly Ser 
            580                 585                 590         
Tyr His Asp Phe Lys Lys Ile Leu Gly Lys Gly Asn Ser Leu Asp Glu 
        595                 600                 605             
Glu Asp Tyr Lys Asp Ile Ile Glu Glu Ile Ile Lys Trp Ile Thr Ile 
    610                 615                 620                 
Phe Asp Asp Lys Lys Met Leu Lys Arg Arg Leu Lys Lys Tyr Pro Glu 
625                 630                 635                 640 
Ile Leu Ser Glu Glu Gln Ile Lys Lys Ile Cys Lys Leu Lys Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Thr Gly Val Arg Asp Lys 
            660                 665                 670         
Glu Thr Gly Glu Ser Ile Ile Asn Ala Leu Trp Glu Thr Asp Pro Ser 
        675                 680                 685             
Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Gln Asp Tyr Gly Phe Gln 
    690                 695                 700                 
Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Glu Asn Ser Tyr 
705                 710                 715                 720 
Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Ala Ile 
                725                 730                 735     
Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Val Lys Val Met Gly His 
            740                 745                 750         
Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Glu Lys Gln Gln Asn 
        755                 760                 765             
Lys Arg Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu Tyr Lys 
    770                 775                 780                 
Ser Cys Lys Asn Asp Ala Thr Asn Leu Trp Lys Glu Leu Leu Arg Asp 
785                 790                 795                 800 
Asp Asn Glu Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr Gln Met 
                805                 810                 815     
Gly Lys Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Asp Asp Leu Phe 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Val Arg Lys Glu Leu Asn Ala Lys Lys 
    850                 855                 860                 
Thr Asp Asn Leu Pro Ser Pro Glu Ile Arg Glu Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Met Leu Leu Asn Lys Gly Phe Ile Ser Lys Lys Lys Tyr Asp 
                885                 890                 895     
Arg Leu Thr Arg Thr Thr Arg Gly Glu Phe Thr Glu Asp Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala 
        915                 920                 925             
Val Ala Glu Leu Leu Lys Gln Leu Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Lys Asn Thr Glu Ile Val Tyr Val Lys Ala Asn Leu 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Asp Leu Ile Lys Cys Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val Gly 
            980                 985                 990         
Asn Val Tyr His Val Lys Tyr Thr Lys Asn Ala Ala Lys Phe Ile Lys 
        995                 1000                1005            
Gly Asn Arg Gly Arg Asn Tyr Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Met His Phe Tyr Gly Asn Ile Lys Lys Met Phe Thr Ser Asp Val 
1025                1030                1035                1040
Pro Ile Ala Asp Arg Asn Gly Glu Ile Val Trp Asp Lys Asp Lys Ser 
                1045                1050                1055    
Ile Ala Thr Val Arg Lys Val Met Asn Lys Asn Asn Ile Leu Tyr Thr 
            1060                1065                1070        
Arg Tyr Thr Tyr Glu Glu Lys Gly Gly Leu Phe Asp Gln Thr Ile Leu 
        1075                1080                1085            
Lys Lys Gly Asn Ser Gln Lys Leu Ile Pro Leu Lys Lys Asn Leu Asp 
    1090                1095                1100                
Pro Glu Lys Tyr Gly Gly Tyr Asn Ser Pro Thr Ala Ala Tyr Phe Ala 
1105                1110                1115                1120
Leu Val Glu Tyr Asp Ile Glu Glu Gly Lys Lys Lys Lys Glu Lys Thr 
                1125                1130                1135    
Thr Lys Gln Leu Val Pro Ile Pro Val Tyr Glu Lys Ala Lys Leu Glu 
            1140                1145                1150        
Ser Asn Ala Ile Lys Tyr Leu Glu Glu Arg Gly Leu Lys Asn Pro Lys 
        1155                1160                1165            
Val Asn Leu Leu Ile Lys Ile Lys Lys Asn Ser Leu Leu Glu Met Asp 
    1170                1175                1180                
Gly Tyr Arg Met Arg Ile Ser Gly Ala Thr Ile Ile Phe Lys Asn Ala 
1185                1190                1195                1200
Val Gln Leu Val Leu Asp Glu Glu Asn Tyr Lys Ile Leu Lys Lys Val 
                1205                1210                1215    
Ala Lys Val Leu Glu Lys Asn Glu Asn Gln Thr Ile Gly Tyr Thr Glu 
            1220                1225                1230        
Glu Leu Leu Ala Leu Tyr Glu Glu Leu Leu Asp Lys Leu Lys Asp Tyr 
        1235                1240                1245            
Lys Asn Arg Leu Ala Asn Gln Ala Lys Thr Leu Glu Lys Gly Lys Glu 
    1250                1255                1260                
Ala Phe Glu Lys Leu Asn Leu Glu Asp Lys Ala Glu Val Ile Asn Glu 
1265                1270                1275                1280
Ile Ile Arg Leu Phe His Cys Asn Ala Thr Thr Ala Asn Asn Ala Leu 
                1285                1290                1295    
Lys Leu Ile Gly Gly Ser Lys Asn Arg Met Thr Asn Asn Asn Ile Ser 
            1300                1305                1310        
Lys Ser Asn Lys Val Ser Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Lys Ile Asp Leu Gly Lys Ile Gly Glu Asp 
    1330                1335                1340

<210> 68
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #65

<400> 68
Met Lys Lys Glu Tyr Tyr Leu Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Val Lys Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Ile Arg Leu Phe Asp Thr Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Tyr Arg Ser Ser Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ser Glu Glu Ile Ala Lys Val Asp Pro Thr 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Glu Asp Lys Tyr Pro Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Ile Glu 
    130                 135                 140                 
Asn Pro Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Ile Glu Gly Asp Ser Asp Ser 
                165                 170                 175     
Lys Asn Thr Asp Leu Ser Glu Val Phe Ser Glu Leu Val Lys Val Phe 
            180                 185                 190         
Asn Asp Ala Leu Glu Ile Glu Leu Leu Ser Thr Glu Ala Leu Glu Leu 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Lys Ser Ala Lys Ala Lys Glu 
    210                 215                 220                 
Val Ala Lys Leu Phe Ala Asp Glu Lys Glu Gln Gln Cys Ile Lys Ala 
225                 230                 235                 240 
Leu Ser Lys Leu Ile Val Gly Leu Thr Gly Asn Ile Ser Glu Ile Phe 
                245                 250                 255     
His Gln Ser Glu Asp Thr Ser Ile Asn Phe Ser Asp Ser Asn Tyr Glu 
            260                 265                 270         
Glu Asn Val Glu Ala Ile Glu Asp Thr Ile Pro Asp Glu Phe Ser Glu 
        275                 280                 285             
Leu Ile Asp Ala Ile Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Gly 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asn Leu Lys Gln Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Thr Tyr Leu Pro Glu Glu Tyr Lys Glu Phe Phe Ser 
            340                 345                 350         
Ser Asp Glu Lys Asn Gly Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Glu Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Lys Glu Lys Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Ala Glu Ile Glu Asn Glu Thr Leu Leu Pro 
385                 390                 395                 400 
Leu Gln Arg Ser Lys Asp Asn Gly Thr Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Leu Glu Leu Lys Lys Ile Leu Glu Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Ile Ser Ile Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Gly Asn Gly Ser Asn Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Glu Asp Gly Lys Ile Tyr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Val Val Asp Val Glu Lys Ser Asn Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Lys 
        515                 520                 525             
Ile Arg Gly Asn Lys Ile Lys Trp Arg Arg Phe Ser Val Glu Leu Lys 
    530                 535                 540                 
Gln Glu Ile Tyr Asn Asp Leu Phe Cys Arg Asn Arg Lys Val Thr Gly 
545                 550                 555                 560 
Lys Lys Ile Leu Asp Tyr Leu Lys Arg Glu Tyr Gly Glu Leu Asp Glu 
                565                 570                 575     
Ala Asp Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ala Leu Ala Ser 
            580                 585                 590         
Tyr His Asp Phe Lys Lys Leu Leu Gly Lys Gly Asn Ser Leu Asp Glu 
        595                 600                 605             
Glu Asp Tyr Lys Glu Ile Ile Glu Glu Ile Ile Lys Trp Lys Thr Ile 
    610                 615                 620                 
Tyr Asp Asp Asn Lys Met Leu Lys Arg Met Leu Lys Lys Tyr Pro Glu 
625                 630                 635                 640 
Ile Leu Ser Glu Glu Gln Ile Lys Lys Ile Cys Lys Leu Lys Tyr Thr 
                645                 650                 655     
Gly Trp Gly Asn Phe Ser Arg Glu Phe Leu Thr Gly Val Arg Gly Lys 
            660                 665                 670         
Glu Thr Gly Glu Ser Ile Ile Asn Ala Leu Trp Glu Thr Asp Pro Ser 
        675                 680                 685             
Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Gln Asp Tyr Ser Phe Gln 
    690                 695                 700                 
Glu Glu Ile Asp Gln Ile Asn Ala Glu Lys Ala Gly Glu Asn Ser Tyr 
705                 710                 715                 720 
Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Asn Lys Arg Ala Ile 
                725                 730                 735     
Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Val Lys Val Met Gly His 
            740                 745                 750         
Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Gly Lys Glu Lys Asn 
        755                 760                 765             
Lys Lys Arg Thr Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu Tyr Lys 
    770                 775                 780                 
Ser Cys Lys Asn Asp Thr Thr Asn Leu Trp Lys Glu Leu Leu Arg Asp 
785                 790                 795                 800 
Asp Asn Glu Leu Arg Ser Lys Lys Leu Phe Leu Tyr Tyr Thr Gln Met 
                805                 810                 815     
Gly Lys Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Asp Asp Leu Leu 
            820                 825                 830         
Asn Trp Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Met Val Leu Val Arg Lys Glu Leu Asn Ala Lys Lys 
    850                 855                 860                 
Thr Asn Asn Leu Leu Ser Pro Glu Ile Arg Glu Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Met Leu Leu Asn Lys Gly Phe Ile Ser Lys Lys Lys Tyr Asp 
                885                 890                 895     
Arg Leu Thr Arg Thr Thr Arg Gly Glu Phe Thr Glu Glu Glu Leu Ser 
            900                 905                 910         
Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala 
        915                 920                 925             
Val Ala Glu Leu Leu Lys Gln Leu Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Lys Asn Ser Glu Val Val Tyr Val Lys Ala Asn Leu 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Asp Leu Leu Lys Cys Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val Gly 
            980                 985                 990         
Asn Val Tyr His Val Lys Tyr Thr Lys Asn Pro Ala Lys Trp Ile Lys 
        995                 1000                1005            
Gly Asn Arg Asp Ser Asn Tyr Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Met His Phe Tyr Gly Ser Ile Lys Lys Met Phe Thr Ser Asp Val 
1025                1030                1035                1040
Lys Asn Ala Asp Arg Asn Gly Glu Ile Val Trp Asn Lys Asp Lys Ser 
                1045                1050                1055    
Ile Asp Gln Ile Arg Lys Ile Met Asn Lys Asn Asn Ile Leu Tyr Thr 
            1060                1065                1070        
Glu Tyr Thr Tyr Cys Glu Lys Gly Gln Leu Phe Asn Gln Thr Ile Leu 
        1075                1080                1085            
Lys Lys Gly Asn Ser Glu Lys Leu Ile Pro Leu Lys Lys Asn Leu Asp 
    1090                1095                1100                
Pro Glu Lys Tyr Gly Gly Tyr Thr Ser Pro Lys Ala Ser Tyr Phe Ala 
1105                1110                1115                1120
Leu Val Glu Phe Asp Ile Glu Glu Gly Lys Lys Lys Lys Glu Lys Thr 
                1125                1130                1135    
Thr Lys Gln Leu Ile Pro Val Pro Val Tyr Glu Lys Ala Lys Leu Glu 
            1140                1145                1150        
Ser Asn Ala Ile Lys Tyr Leu Glu Glu Arg Gly Leu Thr Asn Val Val 
        1155                1160                1165            
Val Asn Leu Lys Ile Lys Ile Lys Lys Asn Ser Leu Leu Glu Met Asp 
    1170                1175                1180                
Gly Tyr Arg Met Arg Ile Arg Gly Ala Thr Ile Ile Phe Lys Asn Ala 
1185                1190                1195                1200
Val Gln Leu Val Leu Asp Glu Glu Asn Tyr Glu Ile Leu Arg Lys Val 
                1205                1210                1215    
Glu Lys Tyr Leu Glu Lys Asn Glu Asn Gln Thr Ile Gly Phe Thr Glu 
            1220                1225                1230        
Glu Leu Leu Ser Leu Tyr Glu Glu Leu Leu Asp Lys Leu Lys Asp Tyr 
        1235                1240                1245            
Lys Asn Arg Pro Ala Asn Gln Ala Lys Thr Leu Glu Lys Gly Lys Glu 
    1250                1255                1260                
Ala Phe Glu Ser Leu Asn Leu Glu Asp Lys Ala Glu Val Ile Asn Glu 
1265                1270                1275                1280
Ile Ile Arg Leu Phe His Cys Asn Ala Thr Thr Ala Ser Asn Ala Leu 
                1285                1290                1295    
Lys Leu Ile Asn Gly Ser Lys Asn Arg Met Thr Asn Lys Asn Ile Leu 
            1300                1305                1310        
Lys Ser Asn Lys Val Lys Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Lys Ile Asp Leu Gly Lys Ile Gly Glu Asp 
    1330                1335                1340

<210> 69
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #66

<400> 69
Met Lys Lys Glu Tyr Tyr Ile Gly Leu Asp Met Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Cys Lys Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Thr Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ser Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ser Glu Glu Ile Ser Lys Val Asp Pro Ser 
                85                  90                  95      
Phe Phe Leu Arg Leu Asn Glu Ser Lys Leu Tyr Pro Glu Asp Lys Thr 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Met Glu 
    130                 135                 140                 
Asn Asp Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Phe Glu Gly Asp Leu Ser Ser 
                165                 170                 175     
Lys Asn Thr Asp Phe Gly Ala Val Phe Ser Gln Leu Leu Lys Val Ile 
            180                 185                 190         
Asn Asp Glu Leu Glu Ile Glu Leu Leu Ser Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Lys Ser Lys Ser Ala Lys Ala Thr Glu 
    210                 215                 220                 
Val Val Lys Leu Leu Ala Ser Glu Lys Lys Gln Ala Cys Ile Lys Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Ser Gly Leu Thr Val Lys Leu Ser Glu Ile Phe 
                245                 250                 255     
Gly Asp Gly Glu Arg Thr Ser Ile Ser Phe Ala Asp Ser Asn Tyr Glu 
            260                 265                 270         
Glu Asn Val Glu Ala Ile Glu Asp Thr Leu Pro Glu Arg Phe Ser Glu 
        275                 280                 285             
Leu Ile Glu Ala Ile Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Thr Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Thr Tyr Leu Pro Glu Glu Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Lys Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Asn Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Leu Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Ile Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Arg Gly Asn Gly Glu Asn Ala Trp 
    450                 455                 460                 
Met Val Arg Lys Glu Asp Gly Lys Ile Tyr Pro Trp Asn Phe Asp Asn 
465                 470                 475                 480 
Val Val Asp Val Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Glu Lys Ile Lys Trp Arg Arg Phe Ser Val Glu Leu Lys 
    530                 535                 540                 
Gln Glu Ile Tyr Asn Asp Leu Phe Cys Arg Asn Arg Lys Val Thr Gly 
545                 550                 555                 560 
Lys Lys Leu Lys Asp Tyr Leu Lys Arg Glu Gly Gly Glu Leu Lys Glu 
                565                 570                 575     
Ala Glu Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ser Leu Arg Ser 
            580                 585                 590         
Tyr His Asp Phe Lys Lys Ile Leu Gly Lys Gly Asn Ser Leu Asp Glu 
        595                 600                 605             
Glu Asp Thr Lys Asp Ile Ile Glu Glu Ile Ile Lys Asn Ile Thr Ile 
    610                 615                 620                 
Phe Asp Asp Lys Lys Met Leu Lys Arg Arg Leu Lys Lys Tyr Pro Glu 
625                 630                 635                 640 
Ile Leu Ser Glu Glu Gln Ile Lys Lys Ile Cys Lys Leu Lys Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Thr Glu Val Arg Asp Lys 
            660                 665                 670         
Glu Thr Gly Glu Asn Ile Ile Asn Ala Leu Trp Glu Thr Asp Pro Ser 
        675                 680                 685             
Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Gln Asp Tyr Gly Phe Gln 
    690                 695                 700                 
Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Glu Asn Ser Tyr 
705                 710                 715                 720 
Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Ala Ile 
                725                 730                 735     
Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Val Lys Val Met Gly His 
            740                 745                 750         
Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Glu Lys Gln Gln Asn 
        755                 760                 765             
Lys Arg Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu Tyr Lys 
    770                 775                 780                 
Ser Cys Lys Asn Asp Ala Thr Asp Leu Trp Lys Glu Leu Leu Arg Asp 
785                 790                 795                 800 
Asp Asn Glu Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr Gln Met 
                805                 810                 815     
Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp Leu Phe 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Val Lys Lys Glu Leu Asn Ala Lys Lys 
    850                 855                 860                 
Thr Asp Asn Tyr Pro Ser Pro Glu Ile Arg Glu Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Met Leu Leu Asn Lys Gly Phe Ile Ser Lys Lys Lys Tyr Asp 
                885                 890                 895     
Arg Leu Thr Arg Thr Thr Arg Gly Glu Phe Thr Glu Asp Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala 
        915                 920                 925             
Val Ala Glu Leu Leu Lys Gln Leu Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Lys Asn Thr Glu Ile Val Tyr Val Lys Ala Asn Leu 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Asp Phe Ile Lys Cys Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val Gly 
            980                 985                 990         
Asn Val Tyr His Val Lys Tyr Thr Lys Asn Ala Ala Lys Phe Ile Lys 
        995                 1000                1005            
Gly Asn Pro Gly Arg Asn Tyr Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Met His Phe Tyr Gly Asn Leu Lys Lys Met Phe Thr Ser Asp Val 
1025                1030                1035                1040
Pro Ile Ala Asp Arg Asn Gly Glu Ile Val Trp Asp Lys Asp Lys Ser 
                1045                1050                1055    
Ile Ala Thr Val Arg Lys Val Met Asn Lys Asn Asn Ile Leu Tyr Thr 
            1060                1065                1070        
Arg Tyr Ser Tyr Glu Glu Lys Gly Gly Leu Phe Asp Gln Gln Ile Leu 
        1075                1080                1085            
Lys Lys Gly Asn Gly Gln Ala Leu Ile Pro Leu Lys Lys Arg Leu Asp 
    1090                1095                1100                
Ile Glu Lys Tyr Gly Gly Tyr Asn Lys Pro Thr Ala Ala Tyr Phe Ala 
1105                1110                1115                1120
Leu Val Glu Tyr Asp Ile Glu Glu Gly Lys Lys Gly Lys Glu Lys Thr 
                1125                1130                1135    
Thr Lys Gln Leu Val Pro Ile Pro Val Tyr Glu Lys Ala Lys Leu Glu 
            1140                1145                1150        
Ser Asn Ala Ile Lys Tyr Leu Glu Glu Arg Gly Leu Lys Asn Pro Lys 
        1155                1160                1165            
Val Asn Leu Leu Ile Lys Ile Lys Lys Asn Ser Leu Leu Glu Val Asp 
    1170                1175                1180                
Gly Phe Arg Met His Ile Ser Gly Arg Thr Ile Ile Phe Lys Ser Ala 
1185                1190                1195                1200
Val Gln Leu Val Leu Asp Glu Glu Asn Glu Lys Ile Leu Lys Lys Val 
                1205                1210                1215    
Ala Lys Val Leu Glu Lys Gln Glu Asn Gln Lys Ile Gly Tyr Thr Glu 
            1220                1225                1230        
Glu Leu Leu Ala Leu Tyr Glu Glu Leu Leu Asp Lys Leu Lys Asp Tyr 
        1235                1240                1245            
Lys Ile Arg Leu Ala Asn Gln Ala Lys Thr Leu Glu Asn Gly Lys Glu 
    1250                1255                1260                
Lys Phe Glu Lys Leu Asn Leu Glu Asp Lys Cys Gln Val Ile Asn Glu 
1265                1270                1275                1280
Ile Leu Arg Leu Phe His Cys Asn Ala Thr Thr Ala Asn Asn Ala Leu 
                1285                1290                1295    
Ser Leu Ile Gly Gly Ala Lys Asn Ile Met Thr Asn Asn Asn Ile Ser 
            1300                1305                1310        
Lys Ser Asn Lys Val Ser Leu Ile Asn Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Lys Ile Asp Leu Gly Lys Ile Gly Glu Asp 
    1330                1335                1340

<210> 70
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #67

<400> 70
Met Lys Lys Glu Tyr Tyr Val Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Ile Cys Lys Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Glu Thr Ala Lys Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ser Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Lys Val Asp Pro Gly 
                85                  90                  95      
Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro Glu Asp Lys Pro 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Glu 
    130                 135                 140                 
Asn Glu Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Phe Glu Gly Asp Ile Ser Ser 
                165                 170                 175     
Lys Val Thr Asp Phe Gly Ala Ala Phe Ser Gln Phe Leu Lys Val Ile 
            180                 185                 190         
Asn Asp Glu Leu Glu Ile Glu Leu Leu Asp Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Lys Ser Lys Ser Ala Lys Lys Thr Glu 
    210                 215                 220                 
Leu Val Lys Leu Leu Ala Ala Glu Lys Lys Gln Ala Cys Glu Lys Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Ser Gly Cys Thr Val Lys Leu Ser Glu Ile Phe 
                245                 250                 255     
Gly Asp Gly Glu Arg Thr Lys Ile Ser Phe Ala Asp Ser Asn Tyr Glu 
            260                 265                 270         
Glu Tyr Val Ala Ala Val Glu Asp Thr Leu Ala Glu Arg Phe Leu Glu 
        275                 280                 285             
Leu Ile Glu Ala Ala Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Thr Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Thr Tyr Leu Pro Glu Glu Tyr Lys Asp Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Lys Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Lys Glu Asp Phe Tyr Ala Phe Leu Lys Lys Asn Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Leu Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Ile Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Lys Gly Asn Gly Glu Asn Ala Trp 
    450                 455                 460                 
Ala Val Arg Lys Glu Asp Gly Lys Ile Tyr Pro Trp Asn Phe Glu Asn 
465                 470                 475                 480 
Val Ile Asp Val Glu Glu Ser Ala Glu Lys Phe Ile Gly Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asp Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Glu Lys Ile Lys Trp Arg Arg Phe Ser Val Glu Leu Lys 
    530                 535                 540                 
Gln Glu Ile Tyr Glu Asp Val Phe Cys Arg Tyr Arg Lys Val Thr Gly 
545                 550                 555                 560 
Lys Lys Leu Lys Asp Tyr Leu Ile Arg Glu Gly Ile Ile Gly Lys Glu 
                565                 570                 575     
Ala Glu Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ser Leu Arg Ala 
            580                 585                 590         
Tyr His Asp Phe Lys Lys Ile Leu Gly Lys Gly Lys Leu Leu Asp Val 
        595                 600                 605             
Glu Leu Thr Gln Asn Glu Lys Glu Glu Ile Ile Lys Asn Ile Thr Ile 
    610                 615                 620                 
Phe Asp Asp Lys Lys Met Leu Lys Lys Arg Leu Lys Lys Tyr Pro Glu 
625                 630                 635                 640 
Ile Leu Ser Glu Lys Gln Ile Lys Lys Ile Cys Ala Leu Lys Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Glu Glu Val Gln Ala Lys 
            660                 665                 670         
Glu Thr Gly Glu Asn Ile Ile Thr Ala Leu Trp Glu Thr Asp Pro Ser 
        675                 680                 685             
Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Gln Asp Tyr Gly Phe Gln 
    690                 695                 700                 
Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Lys Asn Ser Tyr 
705                 710                 715                 720 
Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Pro Ile 
                725                 730                 735     
Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Glu Lys Val Met Gly His 
            740                 745                 750         
Lys Pro Lys Arg Ile Phe Val Glu Met Ala Arg Glu Lys Gln Gln Asn 
        755                 760                 765             
Lys Arg Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu Tyr Lys 
    770                 775                 780                 
Ser Cys Lys Asn Glu Ala Pro Asp Leu Trp Lys Glu Leu Leu His Asp 
785                 790                 795                 800 
Asp Asn Lys Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr Gln Met 
                805                 810                 815     
Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp Leu Phe 
            820                 825                 830         
Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Met Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Val Lys Lys Glu Leu Asn Ala Lys Lys 
    850                 855                 860                 
Thr Asp Asn Tyr Pro Ser Ala Glu Ile Arg Glu Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Asp Met Leu Leu Asn Lys Gly Phe Ile Ser Lys Glu Lys Tyr Asp 
                885                 890                 895     
Arg Leu Thr Arg Asn Thr Arg Gly Glu Phe Thr Glu Asn Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala 
        915                 920                 925             
Val Ala Glu Leu Leu Lys Gln Ala Tyr Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Lys Glu Thr Glu Ile Val Tyr Val Lys Ala Asn Thr 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Asp Phe Ile Lys Val Arg Asp Val 
                965                 970                 975     
Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val Gly 
            980                 985                 990         
Asn Thr Tyr Tyr Val Lys Phe Thr Lys Asn Ala Ala Trp Phe Ile Lys 
        995                 1000                1005            
Gly Asn Pro Gly Arg Thr Tyr Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Met His Phe Tyr Gly Asn Leu Lys Lys Met Phe Thr Ser Asp Val 
1025                1030                1035                1040
Ala Ile Ala Asp Arg Asn Gly Glu Ile Ala Trp Gly Lys Asp Gly Thr 
                1045                1050                1055    
Ile Val Thr Val Arg Lys Val Met Asn Lys Asn Asn Ile Leu Phe Thr 
            1060                1065                1070        
Arg Arg Ser Tyr Glu Val Lys Gly Gly Leu Phe Asp Gln Gln Ile Met 
        1075                1080                1085            
Lys Lys Gly Lys Gly Gln Ala Leu Ile Pro Val Lys Glu Arg Leu Ser 
    1090                1095                1100                
Ile Glu Lys Tyr Gly Gly Tyr Asn Lys Ala Thr Gly Ala Tyr Phe Met 
1105                1110                1115                1120
Leu Val Glu Ser Asp Ile Glu Glu Gly Lys Lys Gly Lys Glu Lys Thr 
                1125                1130                1135    
Thr Lys Gln Leu Val Pro Ile Pro Val Tyr Glu Lys Ala Lys Leu Glu 
            1140                1145                1150        
Ser Asn Ala Ile Arg Tyr Leu Glu Glu Arg Gly Leu Lys Asn Pro Lys 
        1155                1160                1165            
Val Asn Leu Leu Ile Lys Ile Lys Lys Asp Ser Leu Phe Glu Val Asp 
    1170                1175                1180                
Gly Phe Arg Met His Leu Ser Gly Arg Thr Leu Ile Phe Lys Cys Ala 
1185                1190                1195                1200
Asn Gln Leu Val Leu Asp Glu Glu Asn Glu Lys Ile Leu Lys Lys Val 
                1205                1210                1215    
Ala Lys Val Leu Glu Lys Gln Lys Asn Val Lys Ile Gly Tyr Thr Glu 
            1220                1225                1230        
Glu Leu Leu Ala Lys Tyr Glu Glu Phe Leu Asp Lys Leu Lys Asp Tyr 
        1235                1240                1245            
Asn Ile Arg Leu Ser Thr Gln Ala Lys Thr Leu Glu Asn Gly Lys Glu 
    1250                1255                1260                
Lys Phe Glu Lys Leu Ser Leu Glu Asp Lys Cys Gln Val Ile Asn Glu 
1265                1270                1275                1280
Ile Leu His Leu Phe Gln Cys Gln Ser Thr Ala Ala Asn Asn Ala Leu 
                1285                1290                1295    
Ser Leu Ile Gly Gly Ala Gly Asn Ile Leu Val Asn Asn Asn Ile Ser 
            1300                1305                1310        
Lys Cys Asn Lys Val Ser Ile Ile Asn Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Gln Glu Ile Asp Leu Lys Lys Ile Gly Glu Asp 
    1330                1335                1340

<210> 71
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #68

<400> 71
Met Lys Lys Glu Tyr Tyr Val Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Ser Tyr His Ile Cys Arg Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Glu Thr Ala Lys Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ser Arg Arg Arg Leu Asp Arg Arg Lys Arg Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Lys Val Asp Pro Gly 
                85                  90                  95      
Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro Glu Asp Lys Pro 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Glu 
    130                 135                 140                 
Asn Glu Glu Pro Pro Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Met Ile Lys His Arg Gly His Phe Leu Phe Ala Gly Asp Ile Ser Glu 
                165                 170                 175     
Lys Val Thr Asp Phe Gly Ala Ala Phe Ser Gln Phe Leu Lys Val Ile 
            180                 185                 190         
Lys Asp Glu Leu Asp Ile Glu Leu Leu Asp Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Leu Thr Arg Ser Ala Lys Lys Thr Glu 
    210                 215                 220                 
Leu Val Lys Gly Leu Lys Ala Glu Lys Lys Asn Ala Cys Glu Lys Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Ser Gly Cys Thr Val Lys Leu Ser Glu Ile Phe 
                245                 250                 255     
Gly Asp Gly Glu Arg Pro Lys Ile Ser Phe Ala Asp Ser Asn Tyr Glu 
            260                 265                 270         
Glu Tyr Val Ala Val Val Glu Asp Glu Leu Gly Glu Arg Phe Leu Glu 
        275                 280                 285             
Ile Ile Glu Ala Ala Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Ser Ile Ser Glu Ala Lys Val Arg Thr Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asp Leu Lys Tyr Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Thr Tyr Leu Pro Glu Glu Tyr Lys Asp Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Lys Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Lys Glu Asp Phe Tyr Ala Phe Leu Lys Lys Asn Ile Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Leu Glu Lys Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Leu Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Ile Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Lys Gly Asn Gly Glu Phe Ala Trp 
    450                 455                 460                 
Ala Val Arg Lys Ser Asn Glu Lys Ile Tyr Pro Trp Asn Phe Glu Asn 
465                 470                 475                 480 
Val Ile Asp Val Glu Ala Ser Ala Glu Lys Phe Ile Arg Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asp Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Glu Lys Ile Lys Trp Arg Arg Phe Ser Val Glu Leu Lys 
    530                 535                 540                 
Gln Glu Ile Tyr Glu Asp Val Phe Cys Arg Tyr Arg Lys Val Thr Ala 
545                 550                 555                 560 
Lys Lys Leu Lys Asp Tyr Leu Ile Arg Glu Gly Ile Ile Gly Lys Asp 
                565                 570                 575     
Ala Glu Ile Ser Gly Ile Asp Gly Asp Phe Lys Gly Ser Leu Arg Ala 
            580                 585                 590         
Tyr His Asp Phe Lys Lys Ile Leu Gly Lys Gly Lys Leu Leu Asp Val 
        595                 600                 605             
Glu Leu Thr Gln Asn Glu Lys Glu Glu Ile Ile Leu Asn Ile Thr Leu 
    610                 615                 620                 
Phe Asp Asp Lys Lys Leu Leu Lys Lys Arg Leu Lys Lys Tyr Pro Gln 
625                 630                 635                 640 
Ile Leu Thr Glu Lys Gln Ile Lys Lys Ile Cys Ala Leu Asn Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Glu Glu Val Gln Ala Pro 
            660                 665                 670         
Glu Thr Gly Glu Asn Ile Ile Thr Ala Leu Trp Glu Thr Asp Pro Ser 
        675                 680                 685             
Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Gln Asp Tyr Gly Phe Gln 
    690                 695                 700                 
Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Lys Asn Ser Tyr 
705                 710                 715                 720 
Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Gln Ile 
                725                 730                 735     
Trp Gln Thr Leu Gln Ile Val Lys Glu Ile Glu Lys Val Met Gly Glu 
            740                 745                 750         
Lys Pro Lys Arg Val Phe Val Glu Met Ala Arg Glu Lys Gln Glu Asn 
        755                 760                 765             
Lys Arg Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Asp Leu Tyr Lys 
    770                 775                 780                 
Ser Cys Lys Asn Glu Glu Pro Asp Trp Trp Lys Glu Leu Leu His Glu 
785                 790                 795                 800 
Glu His Lys Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr Gln Lys 
                805                 810                 815     
Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp Leu Trp 
            820                 825                 830         
Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Met Asp Asp 
        835                 840                 845             
Ser Leu Asn Asn Arg Val Leu Val Lys Lys Glu Tyr Asn Ala Lys Lys 
    850                 855                 860                 
Thr Asp Asn Tyr Pro Ser Ala Glu Ile Arg Gln Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Asp Ser Leu Leu Asn Gly Gly Phe Ile Ser Lys Glu Lys Tyr Asp 
                885                 890                 895     
Arg Leu Ile Arg Asn Thr Arg Gly Glu Phe Thr Ala Asn Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Glu Arg Gln Leu Val Glu Thr Arg Gln Ser Thr Lys Ala 
        915                 920                 925             
Val Ala Glu Ile Leu Lys Gln Ala Phe Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Lys Glu Thr Glu Ile Val Tyr Val Lys Ala Lys Thr 
945                 950                 955                 960 
Val Ser Glu Phe Arg Gln Asp Phe Asp Phe Ile Lys Val Arg Asp Val 
                965                 970                 975     
Asn Asp Phe His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val Gly 
            980                 985                 990         
Asn Thr Tyr Tyr Val Lys Phe Thr Lys Asn Ala Ala Trp Phe Ile Lys 
        995                 1000                1005            
Gly Asn Pro Gly Arg Thr Tyr Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Met His Phe Tyr Gly Asn Leu Lys Lys Met Phe Thr Ser Asp Val 
1025                1030                1035                1040
Ala Ile Ala Asp Arg Asn Gly Glu Ile Ala Trp Gly Lys Ser Gly Thr 
                1045                1050                1055    
Ile Val Thr Val Arg Lys Met Met Asn Lys Asn Asn Ile Leu Phe Thr 
            1060                1065                1070        
Arg Arg Ser Tyr Glu Val Lys Gly Gly Leu Phe Asp Gln Gln Ile Met 
        1075                1080                1085            
Lys Lys Gly Lys Gly Gln Ala Leu Ile Pro Val Lys Glu Arg Leu Ser 
    1090                1095                1100                
Ile Glu Lys Tyr Gly Gly Tyr Asn Lys Ala Thr Gly Ala Tyr Phe Met 
1105                1110                1115                1120
Leu Val Glu Ser Asp Ile Glu Glu Gly Lys Lys Gly Lys Glu Lys Thr 
                1125                1130                1135    
Thr Ile Glu Phe Val Pro Ile Pro Leu Tyr Glu Lys Ala Lys Leu Glu 
            1140                1145                1150        
Ser Asn Glu Ala Arg Phe Leu Glu Glu Arg Gly Leu Lys Glu Pro Lys 
        1155                1160                1165            
Val Asn Leu Leu Ile Lys Ile Lys Lys Asp Thr Leu Phe Glu Val Asp 
    1170                1175                1180                
Gly Phe Lys Met His Leu Ser Gly Arg Thr Leu Ile Phe Lys Cys Ala 
1185                1190                1195                1200
Asn Gln Leu Ile Leu Asp Glu Glu Asn Glu Lys Ile Leu Lys Lys Val 
                1205                1210                1215    
Ala Lys Val Ile Glu Arg Gln Lys Asn Val Lys Ile Gly Tyr Thr Glu 
            1220                1225                1230        
Glu Leu Leu Ala Lys Tyr Glu Glu Phe Leu Asp Lys Leu Lys Asn Tyr 
        1235                1240                1245            
Gly Ile Arg Leu Ser Thr Gln Ala Lys Thr Leu Glu Asn Gly Lys Glu 
    1250                1255                1260                
Lys Phe Glu Lys Leu Ser Leu Glu Asp Lys Cys Gln Val Leu Asn Glu 
1265                1270                1275                1280
Ile Leu His Leu Phe Gln Cys Gln Ser Ser Ala Ala Asn Asn Ala Leu 
                1285                1290                1295    
Ser Met Ile Gly Gly Pro Gly Asn Ile Leu Val Asn Asn Asn Ile Ser 
            1300                1305                1310        
Lys Cys Asn Lys Ile Ser Ile Ile Asn Gln Ser Pro Thr Gly Ile Tyr 
        1315                1320                1325            
Glu Gln Glu Ile Asp Leu Lys Lys Ile Gly Glu Asp 
    1330                1335                1340

<210> 72
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #69

<400> 72
Met Glu Lys Glu Tyr Tyr Val Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Ser Tyr His Ile Leu Arg Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Glu Ser Ala Lys Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ser Arg Arg Arg Leu Asp Arg Arg Asn Trp Arg Ile Glu 
65                  70                  75                  80  
Ile Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Lys Val Asp Pro Gly 
                85                  90                  95      
Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro Glu Asp Lys Pro 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Glu 
    130                 135                 140                 
Thr Glu Glu Thr Pro Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Met Met Lys His Arg Gly His Phe Leu Leu Ser Gly Asp Ile Ser Glu 
                165                 170                 175     
Lys Val Thr Glu Phe Gly Thr Thr Phe Ser Gln Phe Leu Glu Asn Ile 
            180                 185                 190         
Lys Asn Glu Leu Asp Phe Glu Leu Leu Asp Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Leu Thr Arg Ser Ala Lys Lys Thr Lys 
    210                 215                 220                 
Leu Val Lys Gly Leu Lys Ala Glu Lys Lys Asn Val Cys Glu Lys Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Leu Ser Gly Gly Thr Val Lys Leu Ser Asp Ile Phe 
                245                 250                 255     
Gly Met Gly Glu Arg Pro Lys Ile Ser Phe Ala Asp Asn Gly Tyr Glu 
            260                 265                 270         
Glu Tyr Val Ala Val Val Glu Asp Glu Leu Gly Glu Arg Phe Tyr Glu 
        275                 280                 285             
Ile Ile Glu Ala Ala Lys Ala Val Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Ser Ile Ser Glu Ala Lys Val Arg Thr Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Lys Ser Asp Leu Lys Tyr Leu Lys 
                325                 330                 335     
Asn Ile Val Lys Lys Tyr Leu Pro Glu Glu Tyr Lys Asp Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Leu Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Lys Glu Glu Phe Tyr Asp Phe Leu Lys Lys Asn Ile Lys Glu Leu Glu 
    370                 375                 380                 
Glu Gln Glu Tyr Leu Gln Glu Glu Leu Glu Lys Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Asn Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Tyr Glu Leu Lys Lys Ile Leu Asp Asn Leu Gly Asp Lys Ile Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Leu Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Lys Gly Asn Gly Glu Phe Ala Trp 
    450                 455                 460                 
Ala Val Arg Lys Ser Asn Glu Lys Ile Tyr Pro Trp Asn Phe Glu Asn 
465                 470                 475                 480 
Val Ile Asp Val Glu Ala Ser Ala Glu Lys Phe Ile Arg Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Met Gly Glu Asp Val Leu Pro Lys Asp Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Leu Asp Gly Glu Lys Ile Lys Trp Arg Arg Phe Ser Val Glu Leu Lys 
    530                 535                 540                 
Gln Lys Ile Tyr Glu Asp Val Phe Cys Arg Tyr Arg Lys Val Thr Ala 
545                 550                 555                 560 
Lys Lys Leu Lys Asn Tyr Leu Ile Arg Glu Gly Ile Ile Gly Lys Asn 
                565                 570                 575     
Val Glu Ile Thr Gly Ile Asp Gly Asp Phe Lys Gly Ser Leu Thr Ala 
            580                 585                 590         
Tyr His Asp Phe Lys Lys Ile Leu Gly Lys Gly Lys Leu Leu Asp Val 
        595                 600                 605             
Glu Leu Thr Gln Asn Glu Lys Glu Glu Ile Ile Leu Asn Ile Thr Leu 
    610                 615                 620                 
Phe Asp Asp Lys Lys Leu Leu Lys Lys Arg Leu Lys Lys Tyr Pro Gln 
625                 630                 635                 640 
Ile Leu Thr Glu Lys Gln Ile Lys Lys Ile Cys Ala Leu Ser Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Lys Lys Phe Leu Glu Glu Val Thr Ala Pro 
            660                 665                 670         
Glu Thr Gly Glu Asn Ile Ile Thr Ala Leu Trp Glu Thr Asp Pro Ser 
        675                 680                 685             
Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Glu Asp Tyr Arg Phe Gln 
    690                 695                 700                 
Glu Glu Ile Glu Gln Phe Asn Ala Glu Lys Ala Gly Lys Asn Ser Tyr 
705                 710                 715                 720 
Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Gln Ile 
                725                 730                 735     
Trp Gln Thr Leu Gln Ile Val Lys Glu Ile Glu Lys Val Met Gly Glu 
            740                 745                 750         
Lys Pro Lys Arg Val Phe Val Glu Met Ala Arg Glu Lys Gln Glu Asn 
        755                 760                 765             
Lys Arg Gly Arg Glu Ser Arg Lys Lys Arg Leu Ser Asp Leu Tyr Lys 
    770                 775                 780                 
Ser Cys Lys Asn Glu Glu Pro Asp Trp Trp Lys Glu Leu Leu His Glu 
785                 790                 795                 800 
Glu His Lys Leu Arg Ser Asp Lys Leu Tyr Leu Tyr Tyr Thr Gln Lys 
                805                 810                 815     
Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp Leu Trp 
            820                 825                 830         
Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Thr Met Asp Asp 
        835                 840                 845             
Ser Leu Asn Asn Arg Val Leu Val Lys Lys Glu Tyr Asn Ala Lys Lys 
    850                 855                 860                 
Ser Asp Asn Tyr Pro Ser Ala Asp Ile Arg Gln Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Lys Ser Leu Leu Asn Gly Gly Phe Ile Ser Lys Glu Lys Tyr Asp 
                885                 890                 895     
Arg Leu Ile Arg Asn Thr Arg Gly Glu Leu Thr Ala Asn Glu Leu Ala 
            900                 905                 910         
Gly Phe Ile Glu Arg Gln Ile Val Glu Thr Arg Gln Ser Thr Lys Ala 
        915                 920                 925             
Val Ala Glu Ile Leu Lys Gln Val Phe Pro Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Lys Glu Thr Glu Ile Val Tyr Val Lys Ala Lys Thr 
945                 950                 955                 960 
Val Ser Lys Phe Arg Gln Asp Phe Asp Phe Ile Lys Val Arg Asp Val 
                965                 970                 975     
Asn Asp Leu His His Ala Lys Asp Ala Tyr Leu Asn Ile Val Val Gly 
            980                 985                 990         
Asn Thr Tyr Tyr Val Lys Phe Thr Lys Asn Ala Ala Trp Phe Ile Lys 
        995                 1000                1005            
Glu Asn Pro Gly Arg Thr Tyr Ala Arg Glu Glu Asn Lys Ala Asn Ala 
    1010                1015                1020                
Lys Met His Phe Tyr Gly Asn Leu Lys Lys Met Phe Thr Ser Asp Val 
1025                1030                1035                1040
Ala Ile Ala Asp Arg Asn Gly Glu Ile Ala Trp Gly Lys Ser Gly Thr 
                1045                1050                1055    
Ile Val Thr Val Arg Lys Met Met Asn Lys Asn Asn Ile Leu Val Thr 
            1060                1065                1070        
Arg Arg Ser Tyr Glu Val Lys Gly Gly Leu Phe Asp Gln Gln Ile Met 
        1075                1080                1085            
Lys Lys Gly Lys Gly Gln Ala Leu Ile Pro Val Lys Glu Arg Leu Ser 
    1090                1095                1100                
Ile Glu Lys Tyr Gly Gly Tyr Asn Lys Ala Ala Gly Ala Tyr Phe Met 
1105                1110                1115                1120
Leu Val Glu Ser Asp Ile Glu Glu Gly Lys Lys Gly Lys Glu Lys Thr 
                1125                1130                1135    
Thr Ile Glu Phe Val Pro Ile Pro Leu Tyr Glu Lys Ala Lys Leu Glu 
            1140                1145                1150        
Ser Asp Glu Ala Arg Phe Leu Glu Glu Arg Gly Leu Lys Glu Pro Lys 
        1155                1160                1165            
Val Asn Ile Leu Ile Lys Lys Ile Lys Asp Thr Leu Phe Glu Val Asp 
    1170                1175                1180                
Gly Phe Lys Met Trp Leu Ser Gly Arg Thr Leu Ile Phe Lys Cys Ala 
1185                1190                1195                1200
Asn Gln Leu Ile Leu Asp Glu Glu Asn Met Lys Thr Leu Lys Lys Val 
                1205                1210                1215    
Val Lys Phe Ile Gln Arg Gln Lys Asn Val Lys Ile Gly Ile Thr Glu 
            1220                1225                1230        
Glu Val Leu Met Lys Tyr Glu Thr Phe Leu Asp Lys Leu Lys Asn Tyr 
        1235                1240                1245            
Gly Ile Arg Leu Ser Thr Gln Ala Lys Thr Leu Val Asn Gly Lys Glu 
    1250                1255                1260                
Lys Phe Glu Lys Leu Ser Leu Glu Asp Lys Cys Gln Val Leu Tyr Glu 
1265                1270                1275                1280
Ile Leu His Leu Phe Gln Cys Gln Ser Ser Ala Ala Asn Asn Ala Leu 
                1285                1290                1295    
Lys Met Ile Gly Gly Pro Gly Asn Ile Leu Val Asn Asn Asn Ile Ser 
            1300                1305                1310        
Lys Cys Asn Lys Ile Ser Ile Ile Asn Gln Ser Pro Thr Gly Ile Tyr 
        1315                1320                1325            
Glu Lys Glu Ile Asp Leu Leu Lys Ile Gly Glu Asp 
    1330                1335                1340

<210> 73
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #70

<400> 73
Met Lys Lys Glu Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Ser Ile Lys Lys Asn Leu Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Ser Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Asn Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Phe Val Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Glu Glu Gln His Leu Ser Glu Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Arg Ala Glu Asn 
    210                 215                 220                 
Val Ile Lys Leu Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Val Gly Leu Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Asn Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Arg Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Val Thr Asp Arg Gly Lys Thr Arg Arg Phe Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Glu Asp Tyr Leu Lys Lys Glu Tyr Glu Glu Phe Asp Ser 
                565                 570                 575     
Ala Asp Ile Thr Gly Ile Glu Asp Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Val Ser Lys Asp Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Leu Thr Glu Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ser Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ala Ile Lys Glu Thr Glu Asn Leu Lys 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Arg Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asn Leu Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Glu Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Thr Ile Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Lys Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Arg Lys Asn Asn Ala Arg Glu Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met His Phe Tyr Ser Asn Ile Met Asn Phe Phe Ser Ser Glu Glu 
1025                1030                1035                1040
Pro Ile Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Ile Ala Thr Val Arg Lys Val Leu Ser Tyr His Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Thr Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Thr Tyr Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Tyr Leu Glu Lys Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asn Leu Leu Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Asp Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Glu 
            1220                1225                1230        
Glu His Arg Ala Gln Phe Asp Glu Leu Leu Asp Tyr Ile Ile Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Met Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Asn Ile Asp Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 74
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #71

<400> 74
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Ser Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Asp Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Leu Phe Lys Gln Phe Val Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Leu Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Arg Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Asn Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Asn 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Arg Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Val Asp Asp Arg Gly Lys Thr Gln Arg Phe Ser Ala Glu Glu Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Asp Ser 
                565                 570                 575     
Pro Thr Ile Lys Gly Ile Glu Asp Glu Phe Asn Ala Ser Phe Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Gly Val Ser Lys Glu Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ser Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ala Ile Lys Glu Thr Glu Asn Leu Lys 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Thr Ile Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Asp Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Asn Asn Ala His Glu Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys His Phe Tyr Ser Asn Ile Met Lys Phe Phe Ser Ser Glu Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Ile Ala Thr Val Lys Lys Val Leu Ser Tyr His Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Tyr Leu Glu Lys Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asn Val Leu Met Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Asp Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Glu 
            1220                1225                1230        
Glu His Arg Ala Gln Phe Asp Glu Leu Leu Asp Tyr Ile Ile Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Asn Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Arg Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 75
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #72

<400> 75
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Leu Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Asn Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Asp Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Asp Ser 
                565                 570                 575     
Pro Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Gly Val Ser Lys Glu Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ala Ile Lys Glu Thr Glu Asn Leu Lys 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asn Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Arg Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Asn Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys His Phe Tyr Ser Asn Ile Met Lys Phe Phe Ser Ser Glu Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Ile Ala Thr Ile Lys Lys Val Leu Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Tyr Leu Glu Lys Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asn Val Leu Met Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Tyr Asp Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ala Gln Phe Asp Glu Leu Leu Asp Tyr Val Ile Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Asn Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Leu Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Arg Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 76
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #73

<400> 76
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Pro Met Ser Glu Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Ile Ser Glu Thr Phe Glu Gln Phe Leu Asp Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Leu Val Asp Glu Thr Ile Lys Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ile Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Gly Asn Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Ala His Leu Pro Glu Lys Tyr Tyr Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Ser Lys Thr Asp 
    370                 375                 380                 
Asn Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Asp Arg Gln Ser Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Arg Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Ile Glu Ile Phe Asp Asp Leu Phe Lys Lys Lys Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Val Asn Tyr Leu Glu Asn Glu Tyr Gln Tyr Ile Asp Ser 
                565                 570                 575     
Pro Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Gly Val Ser Lys Glu Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Ile Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Ser Phe Lys 
    690                 695                 700                 
Glu Lys Ile Glu Lys Ala Gln Ala Ile Lys Glu Thr Glu Asn Leu Lys 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Asp Ser Asn Leu Leu Lys Glu Lys Pro Leu Asp 
785                 790                 795                 800 
Asn Glu Gln Leu Lys Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asn Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Arg Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Leu Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Asn Val Asn Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Lys Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Asn Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Asn Asn Met Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys His Phe Tyr Ser Asn Ile Met Lys Phe Phe Ser Ser Glu Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Ile Ser Thr Ile Lys Lys Val Leu Ser Tyr Arg Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Lys Gln Lys Gly Arg Phe Tyr Lys Glu Thr Ile His 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Ile Lys Asn Asn Leu Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asn Glu Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Pro Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Thr Ala Tyr Leu Glu Lys Thr Gly Tyr Asn Asn Pro Lys 
        1155                1160                1165            
Glu Asn Val Leu Met Glu Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Glu Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Tyr Asp Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ala Gln Phe Asp Glu Leu Leu Asp Tyr Val Ile Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Asn Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Asn Ile Ser Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ser Thr Leu Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Arg Leu Glu Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 77
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #74

<400> 77
Met Lys Lys Asn Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Lys Asp Asp Tyr Thr Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Gln Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Pro Met Ser Glu Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Ile Ser Glu Thr Phe Glu Gln Phe Leu Asp Thr Tyr 
            180                 185                 190         
Asn Glu Ile Phe Ser Glu Gln Glu Leu Val Asp Glu Ile Thr Lys Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Gln 
    210                 215                 220                 
Val Leu Ala Leu Phe Pro Asn Glu Lys Arg Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Asp Asp Ile Lys Leu Gln Leu Ser Glu Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Gly Asn Ile Asp Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Asp Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Gln 
305                 310                 315                 320 
Met Ile Glu Leu Tyr Asp Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Ala His Leu Pro Glu Lys Tyr Tyr Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Lys Lys Thr Asp 
    370                 375                 380                 
Asn Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Ser Phe Tyr Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Leu Arg Ala Ile Leu Asp Arg Gln Ser Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Arg Asp Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Glu Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Ile Val Asp Leu Glu Lys Ser Ala Glu Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys Arg Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Glu Arg Gly Lys Thr Gln Asn Phe Ser Ser Gln Glu Lys 
    530                 535                 540                 
Ile Glu Ile Phe Asp Asp Leu Phe Lys Lys Lys Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Val Asn Tyr Leu Glu Asn Glu Tyr Gln Tyr Ile Asp Ser 
                565                 570                 575     
Pro Asp Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Ser Lys Ile Gly Val Ser Lys Glu Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Ile Lys Ile Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Arg Lys Lys Gln Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Thr Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Lys His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp Glu 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Ser Phe Lys 
    690                 695                 700                 
Glu Lys Ile Glu Lys Ala Gln Ala Ile Lys Glu Thr Glu Asn Ile Lys 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Ile Glu Ile Met Gly Tyr 
            740                 745                 750         
Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Lys Asn Asn Ser Lys Gln Arg Leu Lys Lys Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Asp Ser Asn His Val Lys Glu Lys Pro Leu Asp 
785                 790                 795                 800 
Asn Glu Gln Leu Lys Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asn Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Lys Glu Ile Val Arg Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Gln Leu Leu Arg Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Arg Ala 
            900                 905                 910         
His Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Leu Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Asn Ile Asn Ile Val Leu Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Lys Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Asn Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Asn Asn Met Phe Lys Glu Asp Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys His Phe Tyr Ser Asn Ile Met Lys Phe Phe Ser Lys Glu Glu 
1025                1030                1035                1040
Leu Ile Asn Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Leu Ser Thr Ile Lys Lys Val Leu Ser Tyr Lys Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Lys Gln Lys Gly Arg Leu Tyr Lys Glu Thr Ile His 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Ile Lys Asn Asn Leu Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asn Glu Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Pro Ile Met Asp Arg Lys Ala Phe Glu 
            1140                1145                1150        
Lys His Pro Thr Ala Tyr Leu Glu Lys Thr Gly Tyr Asn Asn Pro Thr 
        1155                1160                1165            
Glu Asn Val Leu His Glu Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Ser Arg Arg Met Ile Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Glu Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Tyr Asp Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ala Gln Phe Asp Glu Leu Leu Asp Tyr Val Ile Asp Phe 
        1235                1240                1245            
Ser Asn Lys Tyr Ile Asn Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asn Glu Ala Asp Ile Lys Glu Leu Val Glu Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Asn Ile Ser Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Gly Ser Thr Leu Ile Phe Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Arg Leu Glu Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 78
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #75

<400> 78
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Asp Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Asn Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Asp Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Asp Ser 
                565                 570                 575     
Pro Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Ile Gly Val Ser Lys Glu Val Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Leu Asp Glu Glu Ile Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ala Ile Thr Glu Thr Glu Asn Leu Lys 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Lys Glu Val Val Lys Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Ser Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Ile Met Lys Phe Phe Ser Ser Glu Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Asp 
                1045                1050                1055    
Ile Ala Thr Ile Lys Lys Val Leu Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Tyr Leu Glu Asn Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asn Val Leu Met Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Tyr Asp Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ala Gln Phe Asp Glu Leu Leu Asp His Val Ile Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Thr Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Asn Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Arg Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 79
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #76

<400> 79
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Arg Met 
            20                  25                  30          
Lys Ile His Gly Asn Thr Asp Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Lys Asp Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asn Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Asn Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Glu Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Asp Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Asn Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ser Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     
Asn Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Lys Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Tyr Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Asp Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Asn Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Glu Cys Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asn Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Asp Ser 
                565                 570                 575     
Pro Thr Ile Lys Gly Ile Glu Asp Asn Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Ile Gly Val Ser Arg Glu Val Leu Asp Asp 
        595                 600                 605             
Glu Asp Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Leu Asp Glu Asp Ile Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Leu Ile Thr Glu Thr Glu Asn Leu Lys 
705                 710                 715                 720 
Asp Ile Val Gln Asn Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Gly Ser Asp Leu Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Lys Glu Val Val Lys Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Thr Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Asp Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Ser Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Ile Met Glu Phe Phe Ser Ser Glu Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Asp 
                1045                1050                1055    
Ile Ala Thr Ile Lys Lys Val Leu Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Thr Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Ile 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Tyr Leu Glu Asn Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asn Val Leu Met Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Tyr Ile Glu Ser Asn Asn Ala Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg His Gln Phe Asp Glu Leu Leu Asp His Val Ile Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Thr Phe Asn Lys Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Asn Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Lys Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 80
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #77

<400> 80
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Arg Met 
            20                  25                  30          
Lys Ile Asn Gly Asn Thr Asp Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Lys Asp Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asn Glu Ser Phe Tyr Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Asn Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Glu Glu Thr Phe Lys Glu Phe Leu Gln Glu Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Leu Gln Pro Pro Val Asp Glu Asp Ile Asp Val 
        195                 200                 205             
Glu Ala Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Asn Leu Phe Pro Thr Glu Lys Ser Asn Gly Met Phe His Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Asn Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ser Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     
Asn Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Phe Glu Ile Phe Arg 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Glu Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Ser Phe Leu Lys Lys Ile Asp Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Tyr Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Glu Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ile Glu Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Asn Ser Ala Thr Arg Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Glu Cys Asn Phe Ser Ser Lys Glu Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asn Leu Phe Lys Lys Asn Arg Lys Val Ser Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Asp Ser 
                565                 570                 575     
Pro Thr Ile Lys Gly Ile Glu Asn Asn Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Ile Gly Val Ser Arg Glu Val Leu Asp Asn 
        595                 600                 605             
Glu Asp Tyr Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Leu Asp Glu Asp Ile Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Thr Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ala Leu 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Lys Glu Ile Glu Lys Ala Gln Leu Lys Thr Glu Ser Glu Asn Leu Lys 
705                 710                 715                 720 
Asp Ile Val Asp Asn Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Gln Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Ile Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Gly Ser Asp Leu Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Ser Ile Leu His Gln Arg Phe Asn Thr Glu Asn Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Asp Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Ser Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Leu Met Glu Phe Phe Ser Ser Glu Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Asp 
                1045                1050                1055    
Ile Ala Thr Ile Lys Lys Val Leu Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Thr Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Ile 
1105                1110                1115                1120
Val Ile Thr His Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ile Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Tyr Leu Glu Asn Lys Gly Tyr Val Asn Pro Lys 
        1155                1160                1165            
Glu Asn Val Leu Met Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Tyr Ile Glu Ser Asn Asn Ala Glu Ser Glu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg His Gln Phe Asp Glu Leu Leu Asp His Val Ile Asn Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Thr Leu Ala Asp Ala Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asn Glu Ala Glu Ile Lys Glu Leu Ala Lys Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Thr Phe Asn Lys Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Asn Ile Glu Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Lys Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 81
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #78

<400> 81
Met Lys Lys Gln Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Ser Asp Asp Tyr Asp Leu Ile Arg Lys Arg Met 
            20                  25                  30          
Lys Ile Asn Gly Asp His Asp Lys Gly Lys Met Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Ile 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Arg Arg Arg Arg Asn Arg Ile His 
65                  70                  75                  80  
Tyr Leu Gln Ser Ile Phe Lys Asp Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asn Glu Ser Phe Tyr Val Pro Met Asp Lys Glu 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 
Ser Asn Glu Gln Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Glu Glu Thr Phe Lys Glu Phe Leu His His Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Leu Gln Pro Pro Ile Ser Glu Asp Ile Ser Val 
        195                 200                 205             
Glu Ala Glu Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Asn Lys Phe Gly Thr Glu Lys Ser Asn Gly Met Phe His Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Ser Leu Glu Glu Asp Arg Lys Leu Asn Ile Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Asp Ile Gly Glu Asp Tyr Arg Glu 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Val His Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asp Lys Thr Lys Ala Ser Leu Ser Ser Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Gln Asp Leu Ser Ala Leu Lys 
                325                 330                 335     
Asn Phe Ile Lys Ala His Leu Pro Asp Arg Tyr Phe Asp Ile Phe Arg 
            340                 345                 350         
Asn Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Glu Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Val Leu Asn Lys Val Asp 
    370                 375                 380                 
Gly Ala Glu Ser Phe Leu Lys Lys Ile Asp Glu Glu Arg Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Tyr Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Glu Lys Gln Gly Gln Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Gln Ser Lys Phe Ala Trp 
    450                 455                 460                 
Ile Glu Arg Lys Gly Asp Glu Ser Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Glu Val Asp Leu Val Ser Ser Ala Glu Arg Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Ile Gln Lys Phe Asn Val Phe Asn Glu Leu Thr Lys Val Gln 
        515                 520                 525             
Tyr Ile Asp Asp Gln Gly Lys Arg Cys Asn Phe Ser Ser Lys Glu Lys 
    530                 535                 540                 
Gln Glu Ile Tyr Asp Asn Leu Phe Lys Lys Asn Arg Thr Val Ser Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asp Tyr Leu Lys Asn Glu Tyr Gln Tyr Leu Asp Asn 
                565                 570                 575     
Pro Thr Ile Lys Gly Ile Glu Asn Lys Phe Asn Ala Ser Leu Lys Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Ile Gly Val Ser Arg Glu Val Leu Asp Asn 
        595                 600                 605             
Glu Asn Tyr Glu Glu Arg Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Phe Ser Asp 
625                 630                 635                 640 
Val Leu Asp Gln Asp Ile Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Glu 
            660                 665                 670         
Gln Thr Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ala Pro 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asp Leu Ser Phe Lys 
    690                 695                 700                 
Lys Thr Ile Glu Lys Ala Arg Leu Lys Thr Glu Ser Asp Asn Leu Lys 
705                 710                 715                 720 
Asp Ile Val Asp Lys Ile Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Glu Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Gln Gly Gln Arg Asn Ala Lys Glu Arg Leu Lys Arg Ile Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Lys Ser Asp Leu Leu Lys Lys His Pro Val Asp 
785                 790                 795                 800 
Gln Glu Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Asn Gln Glu Leu Asp Ile Asn Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Thr Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Glu Glu Val Val Lys Asn Met Lys Pro Phe 
865                 870                 875                 880 
Trp Ser Ser Leu Tyr Arg Ser Asn Leu Ile Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Ser Ile Leu His Gln Arg Tyr Asn Thr Glu Asn Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Ala Phe Asp Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Leu Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Asp Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Arg Lys Thr Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Leu Ile Glu Arg Phe Ser Ser Glu Glu 
1025                1030                1035                1040
Gln Ile Ile Asp Glu Asn Gly Glu Ile Val Trp Asp Lys Ser Lys Asp 
                1045                1050                1055    
Ile Ala Lys Ile Lys Arg Val Leu Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Thr Gly Asn Phe Ser Lys Glu Thr Val Gln 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Lys Asp Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Ile 
1105                1110                1115                1120
Ile Phe Thr His Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Ile Gly Ile Thr Ile Met Asp Gln Ser Ile Phe Glu 
            1140                1145                1150        
Ala Asp Gln Ile Lys Tyr Leu Glu Asn Lys Gly Tyr Val Asn Pro Lys 
        1155                1160                1165            
Glu Asn Ile Lys Phe Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Asn Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Thr His Leu Val Thr Leu Leu Tyr His Cys 
                1205                1210                1215    
Lys Arg Tyr Ile Asp Ser Ser Asn Ala Asp His Glu Thr Tyr Ile Asn 
            1220                1225                1230        
Glu Asn Arg His Gln Phe Asn Glu Leu Leu Asp His Val Leu Asn Phe 
        1235                1240                1245            
Ser Ala Gln Tyr Thr Leu Ala Asp Ala Asn Glu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asn Gln Ala Glu Val Ser Glu Leu Ala Thr Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Met Asp Phe Asn Lys Met Gly Ala Pro Ser Ala Phe 
                1285                1290                1295    
Lys Phe Phe Gly Thr Asn Ile Glu Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Lys Glu Val Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 82
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #79

<400> 82
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Thr Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu Asn Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Asp Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Asp Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu Ser 
                565                 570                 575     
Ala Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp Asp 
        595                 600                 605             
Pro Glu Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Ser Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Leu Asp Glu Glu Ile Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ala Ile Thr Asp Thr Glu Asn Leu Gln 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys Pro Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr His Lys Phe Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Ile Met Lys Phe Phe Ala Ser Asp Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Asn 
                1045                1050                1055    
Ile Ala Thr Ile Lys Lys Val Leu Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Arg Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Asn Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asn Val Leu Met Lys Leu Pro Lys Tyr Thr Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Tyr Asp Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ala Glu Phe Ser Glu Ile Leu Asp His Val Ile Asp Phe 
        1235                1240                1245            
Ala Glu Arg Tyr Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Gln Glu Ala Asp Ile Lys Glu Leu Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Arg Leu Gly Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 83
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #80

<400> 83
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Arg Leu Val Ala Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Ala Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Glu Leu Gln Glu Ile Phe Ala Glu Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      
Phe Phe Ala Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Thr Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Lys Ser Tyr 
        115                 120                 125             
His Gln Gln Tyr Pro Thr Ile Tyr His Leu Arg Gln His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Leu Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Gln Phe Ser Glu Pro Glu Pro Val Asp Glu Thr Val Asp Val 
        195                 200                 205             
Ala Val Ile Leu Thr Glu Lys Leu Ser Lys Thr Lys Lys Ala Glu Lys 
    210                 215                 220                 
Val Leu Lys Tyr Phe Pro Asn Glu Lys Ser Asn Gly Tyr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Glu Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Glu Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Gln Ala Lys Asn Val Tyr Asp Ala Ile Leu Leu Ser Glu 
    290                 295                 300                 
Ile Leu Ser Thr Thr Thr Lys Asn Thr Lys Ala Lys Leu Ser Ala Gly 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu Asn Leu Pro Glu Lys Tyr Asn Glu Phe Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Glu Leu Ser Gly Ile Asp 
    370                 375                 380                 
Gly Ser Glu Pro Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Thr Glu Leu Arg Ala Ile Ile Asp Arg Gln Lys Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Ala Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Gln Ser Pro Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Glu Glu Lys Ile Lys Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Met Glu Lys Ser Ala Met Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Met Ile Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Lys Asp Glu Arg Gly Lys Thr His Tyr Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Arg Glu Ile Phe Asp Glu Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Gln Asn Phe Leu Tyr Leu Asn Tyr Asn Tyr Ile Glu Asn 
                565                 570                 575     
Ala Glu Ile Lys Gly Ile Glu Lys Ala Phe Asn Ala Ser Tyr Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Leu Ala Glu Met Gln Glu Trp Leu Glu Asp 
        595                 600                 605             
Pro Glu Met Glu Ser Met Phe Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Thr Gln Leu Ser Lys Tyr Glu Ala 
625                 630                 635                 640 
Val Leu Gly Glu Glu Ile Leu Lys Lys Leu Ala Lys Lys His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Glu Lys Leu Ile Gln Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly Phe 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ala Ile Thr Asp Thr Glu Asn Leu Gln 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Asp Phe Gly Ser Gln Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Pro Leu Asp Ile His Arg Leu Ser 
            820                 825                 830         
Asp Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Arg Asp Asp Val Pro Ser Glu Lys Val Val Lys Lys Met Arg Pro Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Arg Ala Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ile Glu Arg Gly Gly Leu Thr Glu Glu Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Gln Lys Gln Glu Asp Glu 
    930                 935                 940                 
Gly Lys Ala Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Ile Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Ile Ala Leu Leu Lys Lys Tyr Pro Gln Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Leu Lys Phe Asn Ala His Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Glu Phe Tyr Ser Asn Ile Met Lys Phe Phe Glu Ser Asp Glu 
1025                1030                1035                1040
Pro Ile Cys Asp Glu Asn Gly Glu Ile Phe Trp Asp Lys Ser Lys Asn 
                1045                1050                1055    
Ile Ala Gln Ile Lys Lys Val Ile Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Val Gln 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Ser Lys Tyr Gly Gly Phe Gly Ser Pro Asn Val Ala Tyr Thr Val 
1105                1110                1115                1120
Ala Phe Thr Tyr Asp Ile Glu Lys Gly Lys Ala Arg Lys Leu Lys Arg 
                1125                1130                1135    
Ala Lys Ala Ile Glu Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Gln Asn Pro Ile Ala Phe Leu Lys Asn Lys Gly Tyr Glu Asn Ala Lys 
        1155                1160                1165            
Glu Asn Val Phe Met Lys Leu Pro Lys Tyr Thr Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Val Ala Ser His Lys Glu Ala Gln Lys Ala 
1185                1190                1195                1200
Asn Ser Phe Ile Leu Pro Glu His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Tyr Asp Glu Ile Ser His Lys Glu Ser Phe Asp Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ser Glu Phe Ser Glu Ile Leu Asp His Val Ile Asp Phe 
        1235                1240                1245            
Ala Gly Arg Tyr Thr Leu Ala Asp Lys Asn Ile Glu Lys Ile Gln Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Gln Glu Ala Asp Ile Ala Glu Leu Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Asp Val Thr Ile Pro Arg Lys Arg Tyr Thr Ser Leu Thr 
            1300                1305                1310        
Glu Ile Trp Asp Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Arg Met Gly Lys Leu Gly Glu Gln 
    1330                1335                1340

<210> 84
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #81

<400> 84
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Arg Leu Val Ala Lys Lys Met 
            20                  25                  30          
Ser Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Ala Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Glu Leu Gln Glu Ile Phe Ala Glu Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      
Phe Phe Ala Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Thr Arg Phe Pro Ile Phe Ala Thr Leu Glu Glu Glu Lys Ser Tyr 
        115                 120                 125             
His Gln Gln Tyr Pro Thr Ile Tyr His Leu Arg Gln His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Leu Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Glu Leu Asp Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Gln Phe Ser Glu Pro Glu Pro Val Asp Glu Thr Glu Gly Val 
        195                 200                 205             
Ala Val Ile Leu Thr Glu Lys Leu Ser Lys Thr Lys Lys Val Glu Lys 
    210                 215                 220                 
Val Leu Lys Tyr Tyr Pro Asn Glu Lys Ser Asn Gly Tyr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Glu Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Glu Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Gln Ala Lys Asn Val Tyr Asp Ala Ile Leu Leu Ser Glu 
    290                 295                 300                 
Ile Leu Ser Thr Thr Thr Lys Asn Thr Lys Ala Lys Leu Ser Ala Gly 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Lys Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu Asn Leu Pro Glu Lys Tyr Asn Glu Phe Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Glu Leu Ser Gly Ile Asp 
    370                 375                 380                 
Gly Ser Glu Pro Phe Leu Glu Lys Ile Asp Gln Glu Asn Phe Leu Leu 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Ala Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Thr Glu Leu Arg Ala Ile Ile Asp Arg Gln Lys Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Ala Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Gln Ser Pro Phe Ala Trp 
    450                 455                 460                 
Leu Glu Arg Lys Ser Ala Glu Lys Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Met Glu Lys Ser Ala Met Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Met Ile Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Lys Asp Glu Arg Gly Lys Thr His Tyr Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Arg Glu Ile Phe Asp Glu Leu Phe Lys Lys His Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Gln Asn Phe Leu Tyr Leu Asn Tyr Asn Tyr Ile Glu Asn 
                565                 570                 575     
Ala Glu Ile Lys Gly Ile Glu Lys Ala Phe Asn Ala Ser Tyr Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Leu Ala Glu Met Gln Glu Trp Leu Glu Asp 
        595                 600                 605             
Pro Glu Met Glu Ser Met Phe Glu Asp Ile Val Lys Ile Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Thr Gln Leu Ser Lys Tyr Glu Ala 
625                 630                 635                 640 
Val Leu Gly Glu Glu Ile Leu Lys Lys Leu Ala Lys Lys His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Glu Lys Leu Ile Gln Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Asp Phe 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp His Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Ala Lys Ala Leu Ala Ile Ser Asp Thr Gln Ser Leu Leu 
705                 710                 715                 720 
Glu Val Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Trp Gln Ser Leu Lys Ile Val Glu Glu Leu Val Glu Ile Ile Gly Tyr 
            740                 745                 750         
Asn Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Lys Thr 
        755                 760                 765             
Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Asn Phe Gly Ser Pro Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Pro Leu Asp Ile His Arg Leu Ser 
            820                 825                 830         
Glu Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Ile Val Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Arg Asp Asp Val Pro Ser Glu Lys Val Val Lys Lys Met Arg Pro Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Gln Arg Ala Lys Leu Ile Ser Gln Arg Lys Phe Ala 
                885                 890                 895     
Asn Leu Thr Lys Ile Glu Arg Gly Gly Leu Thr Glu Glu Asp Lys Ala 
            900                 905                 910         
Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln His Phe Asn Gln Lys Gln Glu Asp Glu 
    930                 935                 940                 
Gly Lys Ala Ser Gly Lys Val Arg Ile Ile Thr Leu Lys Ser Thr Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Ile Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asn His His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Ile Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asn Tyr Ile Lys Phe Asn Leu His Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Glu Phe Tyr Ser Asn Ile Met Lys Phe Phe Glu Ser Asp Glu 
1025                1030                1035                1040
Pro Ser Cys Asp Glu Asn Gly Glu Ile Phe Trp Asp Lys Ser Arg Asn 
                1045                1050                1055    
Ile Ala Gln Ile Lys Lys Val Ile Ser Ser His Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Tyr Lys Glu Thr Val Asn 
        1075                1080                1085            
Pro Lys Gly Asn Pro Asp Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Ser Lys Tyr Gly Gly Phe Gly Ser Pro Val Val Ala Tyr Thr Val 
1105                1110                1115                1120
Ala Phe Ile Tyr Asp Ile Glu Lys Gly Lys Ala Arg Lys Leu Lys Arg 
                1125                1130                1135    
Ala Lys Ala Ile Glu Gly Ile Thr Ile Met Glu Gln Ser Ala Phe Glu 
            1140                1145                1150        
Gln Asp Pro Ile Ala Phe Leu Lys Asn Lys Gly Tyr Glu Asn Val Lys 
        1155                1160                1165            
Glu Asn Glu Phe Met Lys Leu Pro Lys Tyr Thr Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Val Ala Ser His Lys Glu Ala Gln Lys Ala 
1185                1190                1195                1200
Asn Ser Phe Ile Leu Pro Glu His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Tyr Asp Glu Ile Ser His Lys Glu Ser Phe Asp Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ser Glu Phe Ser Glu Ile Leu Asp His Val Ile Asp Phe 
        1235                1240                1245            
Ala Gly Arg Tyr Thr Leu Ala Asp Lys Asn Ile Glu Lys Ile Gln Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Gln Glu Ala Asp Ile Ala Glu Leu Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Asp Val Thr Ile Pro Arg Lys Arg Tyr Thr Ser Leu Thr 
            1300                1305                1310        
Glu Ile Trp Asp Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Arg Met Gly Asn Leu Gly Glu Gln 
    1330                1335                1340

<210> 85
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #82

<400> 85
Met Thr Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Arg Leu Met Ala Lys Lys Met 
            20                  25                  30          
Ser Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Ala Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Glu Leu Gln Glu Ile Phe Ala Glu Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      
Phe Phe Val Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Gln 
            100                 105                 110         
Tyr Thr Arg Phe Pro Ile Phe Pro Thr Leu Glu Glu Glu Lys Ser Tyr 
        115                 120                 125             
Tyr Gln Gln Tyr Pro Thr Ile Tyr His Leu Arg Gln Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Leu Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Glu Leu Asp Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Glu Val Tyr 
            180                 185                 190         
Asn Gln Gln Phe Asp Gln Pro Glu Ala Val Asp Glu Thr Glu Gly Val 
        195                 200                 205             
Ala Val Ile Leu Thr Glu Lys Leu Ser Lys Thr Lys Lys Val Glu Lys 
    210                 215                 220                 
Ile Leu Lys Tyr Tyr Pro Thr Glu Lys Thr Asn Gly Tyr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Glu Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ser Leu Leu Glu Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Gln Ala Lys Lys Val Tyr Asp Ala Ile Leu Leu Ser Glu 
    290                 295                 300                 
Ile Leu Ser Thr Thr Thr Lys Asn Thr Lys Ala Lys Leu Ser Ala Gly 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Lys Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu Asn Leu Pro Glu Lys Thr Thr Val Phe Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Glu Leu Ser Gly Ile Val 
    370                 375                 380                 
Gly Ser Glu Pro Phe Leu Glu Lys Ile Asp Gln Glu Asn Phe Leu Leu 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Ala Asn Gly Val Ile Pro His Gln Val His Leu 
                405                 410                 415     
Thr Glu Leu Lys Ala Ile Ile Asp Arg Gln Lys Gln Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Glu Glu Ala Arg Glu Lys Ile Ile Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Gln Ser Ser Phe Ala Trp 
    450                 455                 460                 
Leu Glu Arg Lys Thr Ala Glu Lys Ile Thr Pro Trp Asn Phe Thr Glu 
465                 470                 475                 480 
Val Val Asp Met Glu Lys Ser Ala Met Arg Phe Ile Gln Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Thr Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Tyr Met Ile Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Lys Asp Glu Arg Gly Ile Lys His Tyr Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Arg Glu Ile Phe Lys Glu Leu Phe Gln Lys Gln Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Lys Leu Gln Asn Phe Leu Tyr Ala Asn Tyr Asn Tyr Ile Glu Asn 
                565                 570                 575     
Ala Glu Ile Leu Gly Ile Glu Lys Ala Phe Asn Ala Ser Tyr Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Leu Ala Lys Met Gln Glu Trp Leu Glu Gln 
        595                 600                 605             
Pro Glu Met Glu Ser Met Phe Glu Asp Ile Val Lys Ile Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Thr Gln Leu Ser Lys Tyr Gln Ala 
625                 630                 635                 640 
Val Leu Gly Glu Glu Ile Leu Lys Lys Leu Ala Lys Lys His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Glu Lys Leu Ile His Gly Ile Arg Asp Lys 
            660                 665                 670         
Lys Thr Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Asp Phe 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp His Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Ala Lys Ala Leu Ala Leu Ser Asp Asn Gln Ser Leu Leu 
705                 710                 715                 720 
Glu Val Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Trp Gln Ser Leu Lys Ile Val Glu Glu Leu Ile Glu Ile Ile Gly Tyr 
            740                 745                 750         
Asn Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Lys Thr 
        755                 760                 765             
Asn Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Asn Phe Gly Ser Pro Leu Leu Lys Glu Gln Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Pro Leu Asp Ile Asp Arg Leu Ser 
            820                 825                 830         
Glu Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Ile Val Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Arg Asp Asp Val Pro Ser Glu Lys Val Val Lys Lys Met Arg Pro Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Gln Arg Ala Lys Leu Ile Ser Gln Arg Lys Phe Ala 
                885                 890                 895     
Tyr Leu Thr Lys Ile Glu Arg Gly Gly Leu Thr Pro Glu Asp Lys Ala 
            900                 905                 910         
Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln His Phe Asn Gln Lys Gln Glu Asp Glu 
    930                 935                 940                 
Gly Lys Ala Ser Gly Lys Val Arg Ile Ile Thr Leu Lys Ser Thr Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Ile Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asn His His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Ile Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asn Tyr Ile Lys Phe Asn Leu Ala Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Glu Phe Tyr Ser Asn Ile Leu Lys Phe Phe Glu Ser Asp Glu 
1025                1030                1035                1040
Pro Ser Cys Asp Glu Asn Gly Glu Ile Phe Trp Asp Lys Ser Arg His 
                1045                1050                1055    
Ile Pro Gln Ile Lys Lys Val Ile Ser Ser His Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Tyr Lys Glu Thr Val Asn 
        1075                1080                1085            
Pro Lys Gly Lys Pro Asp Lys Leu Ile Gln Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Val Ser Lys Tyr Gly Gly Phe Gly Ser Pro Val Val Ala Tyr Ala Val 
1105                1110                1115                1120
Ala Phe Ile Tyr Asp Ile Glu Lys Gly Lys Ala Arg Lys Leu Lys Lys 
                1125                1130                1135    
Ala Lys Ala Ile Glu Gly Ile Thr Ile Met Glu Gln Ser Ala Phe Glu 
            1140                1145                1150        
Gln Asp Pro Ile Ala Phe Leu Lys Asn Lys Gly Tyr Ser Asn Val Thr 
        1155                1160                1165            
Glu Asn Glu Phe Ile Lys Leu Pro Lys Tyr Thr Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Val Ala Ser His Lys Glu Ala Gln Lys Ala 
1185                1190                1195                1200
Asn Ser Phe Ile Leu Pro Glu Gln Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Gln His Tyr Asp Glu Ile Ser His Lys Glu Ser Phe Asp Tyr Val Asn 
            1220                1225                1230        
Glu His Leu Ser Glu Phe Ser Glu Ile Leu Asp Gln Val Ile Asp Phe 
        1235                1240                1245            
Ala Gly Arg Tyr Thr Leu Ala Asp Lys Asn Ile Glu Lys Ile Gln Glu 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Gln Glu Ala Asp Thr Ala Glu Leu Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Asp Val Thr Ile Pro Arg Lys Arg Tyr Thr Ser Leu Thr 
            1300                1305                1310        
Glu Ile Trp Asp Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Arg Met Gly Asn Leu Gly Glu Gln 
    1330                1335                1340

<210> 86
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #83

<400> 86
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Thr Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Glu Asn Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile His Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Leu Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Gln Gln Ile Phe Asp Asp Leu Phe Lys Gln Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu Ser 
                565                 570                 575     
Ala Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp Asp 
        595                 600                 605             
Pro Glu Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Leu Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ala Ser Thr Asn Thr Glu Asp Leu Gln 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Glu 
    770                 775                 780                 
Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys Ala Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr His Lys Phe Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Ile Met Lys Phe Phe Ala Ser Asp Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Asn 
                1045                1050                1055    
Ile Ala Thr Ile Lys Lys Val Leu Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Arg Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Asn Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asn Val Leu Met Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Cys Asp Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ala Glu Phe Ser Glu Ile Leu Asp His Val Ile Asp Phe 
        1235                1240                1245            
Ala Glu Arg Tyr Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Arg Leu Gly Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 87
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #84

<400> 87
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Glu Asp Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Thr Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Phe Thr Glu Lys Val Ser Arg Ala Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Thr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Thr Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Glu Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Ala Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Lys Asn Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Ser Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile His Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Leu Ala Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Met Leu Tyr Glu Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Gln Gln Ile Phe Asp Asp Leu Phe Lys Gln Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu Ser 
                565                 570                 575     
Ala Thr Ile Lys Gly Ile Glu Asn Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp Asp 
        595                 600                 605             
Pro Glu Asn Glu Glu Met Leu Glu Glu Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Leu Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Glu Lys Ala Gln Ala Glu Thr Asn Thr Glu Asp Leu His 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Arg Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Glu 
    770                 775                 780                 
Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys Ala Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Arg Ser Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr His Lys Phe Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Ile Met Lys Phe Phe Ala Ser Asp Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Asn 
                1045                1050                1055    
Ile Ala Thr Val Lys Lys Val Met Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Ser Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Arg Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Glu Phe Leu Glu Asn Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asn Val Leu Met Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Cys Asp Glu Ser Asn Asn Ser Glu Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ala Glu Phe Ser Glu Ile Leu Asp His Val Lys Asp Phe 
        1235                1240                1245            
Ala Glu Arg Tyr Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Leu Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Asn Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Arg Leu Gly Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 88
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #85

<400> 88
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Glu Asn Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Glu Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Glu Glu Thr Phe Lys Thr Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Leu Phe Thr Glu Lys Val Ser Arg Ala Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Thr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Thr Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Glu Tyr Glu 
            260                 265                 270         
Glu Glu Leu Glu Ala Leu Leu Ala Ile Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Ala Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Lys Glu Leu Pro Glu Lys Tyr Asn Glu Ile Phe Arg 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Ser Val Ser 
        355                 360                 365             
Gln Ala Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Pro 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile His Lys Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asp Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Leu Ala Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Met Leu Tyr Glu Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Ser Glu Glu Lys 
    530                 535                 540                 
Lys Gln Ile Phe Asp Glu Leu Phe Lys Gln Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Leu Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu Ser 
                565                 570                 575     
Ala Thr Ile Lys Gly Ile Glu Asn Ser Phe Asn Ala Ser Tyr Gly Thr 
            580                 585                 590         
Tyr His Asp Phe Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp Asp 
        595                 600                 605             
Pro Glu Asn Glu Glu Met Leu Glu Glu Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Tyr Ser Asp 
625                 630                 635                 640 
Val Phe Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Ala Lys Ala Gln Ala Glu Thr Asn Thr Glu Asp Leu His 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Arg Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Glu 
    770                 775                 780                 
Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu Tyr Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Ile Ala Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Lys Arg Ser Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Leu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
His Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Thr Val Arg Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr His Lys Phe Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Ser Asn Ile Met Lys Phe Phe Ala Ser Asp Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Asn 
                1045                1050                1055    
Ile Ala Thr Val Lys Lys Val Met Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Thr Gly Gly Phe Ser Lys Glu Thr Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Ser Arg Lys Thr Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Arg Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Glu Phe Leu Glu Asn Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asn Val Leu Met Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Cys Asp Glu Ser Asn Asn Gly Lys Ser Leu Glu Tyr Val Asn 
            1220                1225                1230        
Glu His Arg Ala Glu Phe Ser Glu Ile Leu Asp Tyr Val Lys Asp Phe 
        1235                1240                1245            
Ala Glu Arg Tyr Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Leu Lys 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Asn Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Arg Leu Gly Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 89
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #86

<400> 89
Met Asn Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Asp Glu Asn Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Ile Gln Gly Asn Gly Asp Lys Lys Lys Val Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Ala Gly Glu Thr Ala Glu Gly Thr Arg Met 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Gly Glu Met Asp Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Glu Lys Arg 
            100                 105                 110         
Asn Glu Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Lys Glu Thr Phe Lys Thr Phe Leu Gln Ala Tyr 
            180                 185                 190         
Asn Gln Ala Phe Thr Val Gln Glu Pro Val Asn Glu Thr Ile Asp Val 
        195                 200                 205             
Gly Ser Leu Phe Gln Glu Arg Val Ser Arg Gln Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Thr Phe Met Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Thr Phe 
                245                 250                 255     
Gly Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Glu Tyr Glu 
            260                 265                 270         
Glu Glu Leu Glu Gly Leu Leu Ala Ile Val Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Ala Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Lys Asp Ile Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Leu Ile Lys Arg Tyr Glu Glu His Lys Thr Asp Leu Glu Gln Leu Lys 
                325                 330                 335     
Lys Phe Val Arg Lys Glu Leu Pro Glu Lys Tyr Tyr Glu Val Phe Arg 
            340                 345                 350         
Asp Ser Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Ser Val Ser 
        355                 360                 365             
Gln Ala Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Glu Lys Ile Pro 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Arg Lys Gln Ser Arg Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asp Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Thr Asp Gly Ser Ile Arg Pro Trp Asn Phe Thr Glu 
465                 470                 475                 480 
Val Val Asp Glu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Ser Tyr Leu Pro Gln Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Met Leu Tyr Glu Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Glu Thr Gln Asn Phe Ser Ser Val Glu Lys 
    530                 535                 540                 
Lys Glu Ile Phe Asp Glu Leu Phe Lys Lys Asn Arg Lys Val Thr Tyr 
545                 550                 555                 560 
Ala Leu Leu Glu Asn Phe Leu Lys Asn Gln Tyr Met Tyr Ile Glu Thr 
                565                 570                 575     
Ala Glu Ile Lys Gly Ile Glu Lys Ser Phe Asn Ala Ser Tyr Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Gly Val Ser Gln Glu Met Leu Asp Asp 
        595                 600                 605             
Pro Glu Asn Glu Glu Met Phe Glu Glu Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Lys Glu Gln Leu Ser Lys Tyr Ala Asp 
625                 630                 635                 640 
Tyr Phe Glu Ala Gly Val Leu Lys Lys Leu Val Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Lys Lys Thr Ile Leu Asp Tyr Leu Met Arg Asp Asp Asp Leu 
        675                 680                 685             
Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Glu Ser Ile Ala Gln Gln Gln Ala Glu Asp Gln Thr Glu Asp Leu His 
705                 710                 715                 720 
Glu Ile Val Lys Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Thr Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Arg Lys Lys Ser Lys Pro Arg Gln Lys Val Leu Glu Glu 
    770                 775                 780                 
Ala Met Lys Glu Phe Gly Ser Asn Leu Leu Lys Glu Tyr Pro Pro Asp 
785                 790                 795                 800 
Asn Ala Asn Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Lys Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Met Val Leu Val Ser Ser Thr Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Ile Gly Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Lys Arg Ser Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Leu Asn Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
His Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu His Gln Lys Tyr Asn Ser Glu Asp Glu Asp Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Thr Thr Arg Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Gln Ile Tyr Gln Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Arg Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Gln Lys Phe Asn Leu Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Asn Phe Tyr Ser Asn Leu Met Arg Phe Phe Ala Ser Asp Gln 
1025                1030                1035                1040
Pro Lys Val Asp Glu Asn Gly Glu Ile Leu Trp Asp Gln Ser Lys Asn 
                1045                1050                1055    
Ile Ala Met Val Lys Lys Val Met Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Ile Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Gln 
        1075                1080                1085            
Pro Lys Gly Asp Ser Ala Lys Leu Ile Ala Arg Lys Thr Gly Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Ile Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Arg Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Arg Gln Phe Glu 
            1140                1145                1150        
Lys Asn Glu Val Glu Phe Leu Glu Asn Lys Gly Tyr Gln Asn Pro Arg 
        1155                1160                1165            
Glu Asn Val Leu Val Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Ile Ala Ser Ala Thr Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Asn His Leu Val Glu Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Cys Asp Asp Leu Asn Asp Gly Lys Ser Leu Ala Tyr Leu Asn 
            1220                1225                1230        
Ala His Arg Ala Glu Phe Asn Glu Leu Phe Glu Tyr Val Lys His Phe 
        1235                1240                1245            
Ala Glu Arg Tyr Thr Leu Ala Asp Lys Asn Leu Lys Lys Leu Ser Lys 
    1250                1255                1260                
Leu Tyr Glu Gln Asn Gln Glu Ala Asp Ile Lys Val Met Ala Gln Ser 
1265                1270                1275                1280
Phe Leu Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Lys Phe Phe Asp Thr Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Ile Leu Asn Ala Thr Ile Ile Asn Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr His Trp Lys Leu Gly Glu Leu Gly Glu Asp 
    1330                1335                1340

<210> 90
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #87

<400> 90
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Ile Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ala Asn Gly Glu Pro Leu Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Thr Arg Lys Ala Glu Lys 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Lys Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Ile Gln Phe Ala Lys Glu Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Ser 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Glu Asn Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Gln Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Asn Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Gln Ala Ile Ile His Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Leu Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Asn Phe Ser Gly Glu Glu Lys 
    530                 535                 540                 
Gln Gln Ile Phe Asp Asp Leu Phe Lys Gln Lys Arg Lys Val Lys Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Gln Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu Ser 
                565                 570                 575     
Ala Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp Asp 
        595                 600                 605             
Pro Glu Asn Ala Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Phe Ser Asp 
625                 630                 635                 640 
Val Leu Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe Lys 
    690                 695                 700                 
Asn Glu Ile Glu Lys Ala Gln Ala Ser Thr Asn Asp Glu Asp Leu Gln 
705                 710                 715                 720 
Glu Ile Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu Glu Lys 
    770                 775                 780                 
Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Ala Leu Arg Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys Ala Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp Glu Glu 
    930                 935                 940                 
Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr His Lys Phe Asn Ala Phe Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Thr Asn Ile Met Lys Phe Phe Ala Lys Asp Glu 
1025                1030                1035                1040
Pro Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Tyr 
                1045                1050                1055    
Leu Ala Thr Ile Lys Lys Val Leu Ser Tyr His Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Ile Gln Lys Gly Gly Phe Ser Lys Glu Thr Ile Lys 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asn Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Asn Val Ala Tyr Ser Val 
1105                1110                1115                1120
Val Ile Thr Tyr Asp Ile Glu Lys Gly Lys Lys Arg Lys Leu Lys Arg 
                1125                1130                1135    
Val Lys Glu Ile Val Gly Ile Thr Ile Met Glu Arg Thr Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Glu Lys Gly Tyr Gln Asn Pro Lys 
        1155                1160                1165            
Glu Asn Val Leu Met Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Lys Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Cys Asp Glu Ser Asn Asn Ser Glu Ser Leu Ala Tyr Val Glu 
            1220                1225                1230        
Glu His Arg Ala Glu Phe Ser Glu Ile Leu Asp His Val Ile Asp Phe 
        1235                1240                1245            
Ala Glu Arg Tyr Thr Leu Ala Asp Ala Asn Leu Glu Lys Ile Lys Lys 
    1250                1255                1260                
Leu Phe Glu Glu Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Gln Phe Asn Ala Met Gly Ala Pro Ala Ser Phe 
                1285                1290                1295    
Lys Phe Phe Glu Lys Thr Ile Glu Arg Lys Arg Tyr Thr Ser Ile Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Arg Arg Leu Gly Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 91
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #88

<400> 91
Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          
Lys Val Ala Gly Asn Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  
Asn Arg Thr Thr Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Ala Asn 
                85                  90                  95      
Phe Phe Tyr Arg Leu Asp Asp Ser Phe Tyr Val Glu Ser Glu Lys Arg 
            100                 105                 110         
His Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Lys Leu Val Asn 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Lys Leu Asp Thr 
                165                 170                 175     
Lys Asn Thr Ser Val Asp Glu Val Phe Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         
Asn Gln Val Phe Ala Ser Gly Ile Arg Leu Glu Glu Asn Lys Asp Val 
        195                 200                 205             
Ala Lys Ile Leu Ser Glu Lys Val Thr Arg Lys Glu Lys Leu Glu Lys 
    210                 215                 220                 
Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser Thr Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Ile Ser Leu Ile Val Gly Ser Lys Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Asp Glu Lys Thr Asp Ile Glu Cys Ala Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Glu 
        275                 280                 285             
Ile Phe Val Ala Ala Lys Asn Ala Tyr Asn Ala Val Val Leu Ser Ser 
    290                 295                 300                 
Ile Ile Thr Val Thr Asp Thr Glu Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Asp Lys His Glu Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Ala Phe Ile Lys Val His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Asn 
            340                 345                 350         
Asp Val Glu Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Lys 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Met Lys Lys Met Leu Glu Asn Ile Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Ile Asp Lys Ile Glu Glu Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile Pro His Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Glu Ala Ile Leu His Gln Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Tyr Glu Lys Ile Lys Ser Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly Gln Ser Glu Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Lys Val Asp Phe Gly Lys Ser Ala Ile Asp Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Gln Gly Lys Thr His Tyr Phe Ser Gly Gln Glu Lys 
    530                 535                 540                 
Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Lys Arg Lys Val Lys Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Gln Phe Leu Arg Asn Met Ser Asn His Ile Glu Ser 
                565                 570                 575     
Pro Thr Ile Glu Gly Val Glu Asp Ser Phe Asn Ser Ser Phe Ala Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Val Gly Val Thr Gln Glu Ile Leu Asp Asn 
        595                 600                 605             
Pro Leu Asn Ala Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu Gln Gln Phe Ser Asp 
625                 630                 635                 640 
Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Ile Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe Lys 
    690                 695                 700                 
Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Ala Asp Lys Asp Ile Gln 
705                 710                 715                 720 
Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Gly Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Gly Lys Gly Lys Asn Asn Ser Lys Pro Arg Phe Lys Ser Leu Glu Lys 
    770                 775                 780                 
Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Gly Leu Arg Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Ala Asn Arg Glu Lys 
    850                 855                 860                 
Gly Asp Asp Val Pro Ser Leu Glu Val Val Arg Lys Arg Lys Val Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Gln Ala Asn Leu Met Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Cys Glu Lys Asp Glu Asn 
    930                 935                 940                 
Gly Asn Thr Ile Glu Gln Val Arg Ile Val Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr Lys Val Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Asn Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr His Gln Phe Asp Trp Phe Lys Ala Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Thr Asn Ile Met Leu Phe Phe Ala Lys Lys Asp 
1025                1030                1035                1040
Arg Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Tyr 
                1045                1050                1055    
Leu Asp Thr Ile Lys Lys Val Leu Ser Tyr Arg Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Ile Gln Lys Gly Glu Phe Ser Lys Ala Thr Ile Lys 
        1075                1080                1085            
Pro Lys Gly Asn Ser Ser Lys Leu Ile Pro Arg Lys Ala Asn Trp Asp 
    1090                1095                1100                
Pro Met Lys Tyr Gly Gly Phe Asp Ser Pro Asn Met Ala Tyr Ala Val 
1105                1110                1115                1120
Val Ile Glu Tyr Asp Ile Glu Lys Gly Lys Lys Lys Lys Leu Ile Lys 
                1125                1130                1135    
Glu Lys Glu Ile Ile Arg Ile Thr Ile Met Glu Arg Thr Ala Phe Glu 
            1140                1145                1150        
Lys Asp Gln Lys Ala Phe Leu Glu Glu Lys Gly Tyr Arg Asn Pro Lys 
        1155                1160                1165            
Glu Asn Val Leu Thr Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Cys Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Asn Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Asn His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Asn Cys Glu Ala Ser Asn Asp Gly Lys Ser Leu Ala Tyr Ile Glu 
            1220                1225                1230        
Ser His Arg Glu Met Phe Ala Glu Leu Leu Ala His Val Ser Glu Phe 
        1235                1240                1245            
Ala Lys Arg Tyr Thr Leu Ala Asp Ala Asn Leu Glu Lys Ile Asn Gln 
    1250                1255                1260                
Leu Phe Glu Gln Asn Lys Glu Gly Asp Ile Lys Val Ile Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Ala Phe Asn Ala Met Gly Ala Pro Ala Ser Phe 
                1285                1290                1295    
Lys Phe Phe Glu Thr Thr Ile Glu Arg Lys Arg Tyr Asn Asn Leu Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Ala Arg Lys Arg Leu Asp Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 92
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #89

<400> 92
Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          
Lys Val Ala Gly Asn Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  
Asn Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Ile Asp Ala Asn 
                85                  90                  95      
Phe Phe Cys Arg Leu Asn Asp Ser Phe Tyr Val Asp Ser Glu Lys Arg 
            100                 105                 110         
Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
                165                 170                 175     
Lys Asn Thr Ser Val Asp Gly Val Tyr Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         
Asn Gln Val Phe Ala Ser Gly Ile Lys Leu Glu Glu Asn Lys Asp Val 
        195                 200                 205             
Ala Lys Ile Leu Ala Glu Lys Val Thr Arg Lys Glu Lys Leu Glu Arg 
    210                 215                 220                 
Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser Thr Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Ile Ser Leu Ile Val Gly Ser Lys Gly Asn Phe Gln Lys Val Phe 
                245                 250                 255     
Asp Leu Val Glu Lys Thr Asp Ile Glu Cys Ala Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Ile Ile Gly Asp Glu Tyr Ala Glu 
        275                 280                 285             
Leu Phe Val Ala Ala Lys Asn Ala Tyr Asn Ala Val Val Leu Ser Ser 
    290                 295                 300                 
Ile Ile Thr Val Thr Asp Thr Glu Thr Asn Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Asp Thr His Glu Lys Asp Leu Ser Glu Leu Lys 
                325                 330                 335     
Ala Phe Ile Lys Leu His Leu Pro Lys Gln Tyr Glu Glu Ile Phe Ser 
            340                 345                 350         
Asn Val Glu Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Lys 
        355                 360                 365             
Gln Ala Asp Phe Tyr Lys Tyr Met Lys Thr Met Leu Glu Asn Ile Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Ile Ala Lys Ile Glu Glu Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile Pro His Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Glu Ala Ile Leu His Gln Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Tyr Asp Lys Ile Lys Ser Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly Gln Ser Glu Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Lys Val Asp Phe Gly Lys Ser Ala Val Asp Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Gln Gly Lys Thr Asn Tyr Phe Ser Gly Gln Glu Lys 
    530                 535                 540                 
Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Lys Arg Lys Val Lys Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Gln Phe Leu Arg Asn Met Ser His His Ile Glu Ser 
                565                 570                 575     
Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn Ser Ser Tyr Ala Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Val Gly Ile Lys Gln Glu Ile Leu Asp Asn 
        595                 600                 605             
Pro Leu Asn Thr Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu Gln Gln Phe Ser Asp 
625                 630                 635                 640 
Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Ile Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe Lys 
    690                 695                 700                 
Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Ala Asp Lys Asp Ile Gln 
705                 710                 715                 720 
Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Ser Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Gly Lys Gly Lys Asn Asn Ser Arg Pro Arg Tyr Lys Ser Leu Glu Lys 
    770                 775                 780                 
Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Glu Leu Arg Asn Asn Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala Gly Asn Arg Glu Lys 
    850                 855                 860                 
Gly Asp Asp Val Pro Pro Leu Glu Ile Val Arg Lys Arg Lys Val Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Gln Gly Asn Leu Met Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Tyr Glu Lys Asp Asp His 
    930                 935                 940                 
Gly Asn Thr Met Glu Gln Val Arg Ile Val Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr Lys Val Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Asn Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr His Gln Phe Asp Trp Phe Lys Ala Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Thr Asn Ile Met Leu Phe Phe Ala Gln Lys Asp 
1025                1030                1035                1040
Arg Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Tyr 
                1045                1050                1055    
Leu Asp Thr Ile Lys Lys Val Leu Ser Tyr Arg Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Ile Gln Lys Gly Glu Phe Ser Lys Ala Thr Ile Lys 
        1075                1080                1085            
Pro Lys Gly Asn Ser Ser Lys Leu Ile Pro Arg Lys Ala Asn Trp Asp 
    1090                1095                1100                
Pro Met Lys Tyr Gly Gly Leu Asp Ser Pro Asn Met Ala Tyr Ala Val 
1105                1110                1115                1120
Val Ile Glu Tyr Asp Ile Ala Lys Gly Lys Lys Lys Lys Leu Val Phe 
                1125                1130                1135    
Glu Lys Lys Ile Ile Arg Ile Thr Ile Met Glu Arg Lys Ala Phe Glu 
            1140                1145                1150        
Lys Asp Glu Lys Ala Phe Leu Glu Glu Gln Gly Tyr Arg Gln Pro Lys 
        1155                1160                1165            
Glu Asn Val Leu Thr Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Cys Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Asn Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Gln Val Leu Pro Asn His Leu Val Thr Leu Leu His His Ala 
                1205                1210                1215    
Lys Asn Cys Glu Ala Ser Asn Asp Gly Lys Ser Leu Asp Tyr Ile Glu 
            1220                1225                1230        
Ser Asn Arg Glu Met Phe Ala Glu Leu Leu Ala His Val Ser Glu Phe 
        1235                1240                1245            
Ala Lys Arg Tyr Thr Leu Ala Asp Ala Asn Leu Asn Lys Ile Asn Gln 
    1250                1255                1260                
Leu Phe Glu Gln Asn Lys Glu Gly Asp Ile Lys Val Ile Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Ala Phe Asn Ala Met Gly Ala Pro Ala Ser Phe 
                1285                1290                1295    
Lys Phe Phe Glu Thr Thr Ile Glu Arg Lys Arg Tyr Asn Asn Leu Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Ala Arg Lys Arg Leu Asp Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 93
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #90

<400> 93
Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          
Lys Val Ala Gly Asn Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  
Asn Arg Thr Thr Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Ala Glu Met Ser Glu Val Asp Ala Asn 
                85                  90                  95      
Phe Phe Tyr Arg Leu Glu Asp Ser Phe Tyr Ile Glu Ser Glu Lys Arg 
            100                 105                 110         
His Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Lys Leu Val Asn 
    130                 135                 140                 
Ser Ser Asp Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Lys Leu Asp Thr 
                165                 170                 175     
Lys Asn Thr Ser Val Asp Glu Val Phe Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         
Asn Gln Val Phe Ala Ser Asp Ile Arg Leu Glu Glu Asn Lys Glu Val 
        195                 200                 205             
Ala Lys Ile Leu Ser Glu Lys Leu Thr Arg Arg Glu Lys Leu Asp Lys 
    210                 215                 220                 
Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser Thr Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Ile Ser Leu Ile Ile Gly Ser Lys Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Asp Glu Lys Thr Asp Ile Glu Cys Ala Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Glu 
        275                 280                 285             
Ile Phe Val Ala Ala Lys Asn Ala Tyr Asn Ala Val Val Leu Ser Ser 
    290                 295                 300                 
Ile Ile Thr Val Thr Asp Thr Glu Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Asp Lys His Glu Lys Asp Leu Lys Arg Leu Lys 
                325                 330                 335     
Ala Phe Phe Lys Val His Leu Pro Glu Lys Phe Asn Glu Val Phe Asn 
            340                 345                 350         
Asp Val Glu Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Lys 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Met Lys Lys Met Leu Glu Asn Ile Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Ile Asp Gln Ile Glu Glu Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile Pro His Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Glu Ala Ile Leu His Gln Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asp Tyr Glu Lys Ile Lys Ser Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly Gln Ser Glu Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Lys Val Asp Phe Gly Lys Ser Ala Ile Asp Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn Glu Leu Thr Lys Ile Arg 
        515                 520                 525             
Tyr Ile Asp Asp Gln Gly Lys Thr His Tyr Phe Ser Gly Gln Glu Lys 
    530                 535                 540                 
Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Lys Arg Lys Val Lys Lys 
545                 550                 555                 560 
Lys Asp Leu Glu Gln Phe Leu Tyr Asn Met Met Asn His Ile Glu Ser 
                565                 570                 575     
Pro Thr Ile Glu Gly Val Glu Asp Ala Phe Asn Ser Ser Phe Ala Thr 
            580                 585                 590         
Tyr His Asp Leu Gln Lys Val Gly Val Thr Gln Glu Ile Leu Asp Asn 
        595                 600                 605             
Pro Leu Asn Ala Glu Met Leu Glu Glu Ile Val Lys Ile Leu Thr Val 
    610                 615                 620                 
Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu Gln Gln Phe Ser Asp 
625                 630                 635                 640 
Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Ile Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met Asn Asp Asp Gly Leu 
        675                 680                 685             
Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe Lys 
    690                 695                 700                 
Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Ala Asp Lys Asp Ile Gln 
705                 710                 715                 720 
Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Gly Ile Met Gly Tyr 
            740                 745                 750         
Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Gly Lys Gly Lys Asn Asn Ser Lys Pro Arg Phe Thr Ser Leu Glu Lys 
    770                 775                 780                 
Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Gly Leu Lys Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser Phe Ile Thr Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Ala Asn Arg Glu Lys 
    850                 855                 860                 
Gly Asp Asn Val Pro Ser Leu Glu Val Val Arg Lys Arg Lys Val Phe 
865                 870                 875                 880 
Trp Glu Lys Leu Tyr Gln Ala Lys Leu Met Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys Asn 
        915                 920                 925             
Val Ala Asn Ile Leu His Gln Arg Phe Asn Cys Lys Lys Asp Glu Asn 
    930                 935                 940                 
Gly Asn Thr Ile Glu Gln Val Arg Ile Val Thr Leu Lys Ser Ala Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr Lys Val Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val Val Ala 
            980                 985                 990         
Asn Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr His Gln Phe Asp Trp Phe Lys Ala Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Gln Phe Tyr Thr Asn Ile Met Leu Phe Phe Ala Lys Lys Asp 
1025                1030                1035                1040
Arg Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp Lys Ser Lys Tyr 
                1045                1050                1055    
Leu Asp Thr Ile Lys Lys Val Leu Ser Tyr Arg Gln Met Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Ile Gln Lys Gly Glu Phe Ser Asn Ala Thr Val Asn 
        1075                1080                1085            
Pro Lys Gly Asn Ser Ser Lys Leu Ile Pro Arg Lys Ala Asp Trp Asp 
    1090                1095                1100                
Pro Ile Lys Tyr Gly Gly Phe Asp Gly Ser Asn Met Ala Tyr Ala Ile 
1105                1110                1115                1120
Val Ile Glu Tyr Asp Ile Glu Lys Arg Lys Lys Lys Thr Val Ile Lys 
                1125                1130                1135    
Glu Lys Glu Leu Ile Gln Ile Asn Ile Met Glu Arg Thr Ala Phe Glu 
            1140                1145                1150        
Lys Asp Gln Lys Ala Phe Leu Glu Glu Lys Gly Tyr Arg Asn Pro Lys 
        1155                1160                1165            
Glu Asn Val Leu Thr Lys Ile Pro Lys Tyr Thr Leu Tyr Glu Cys Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Gly Ser Ala Asn Glu Ala Gln Lys Gly 
1185                1190                1195                1200
Asn Gln Met Val Leu Pro Asn His Leu Met Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Asn Cys Glu Ala Ser Asn Asp Gly Lys Ser Leu Ala Tyr Ile Glu 
            1220                1225                1230        
Ser His Arg Glu Met Phe Ala Glu Leu Leu Ala His Ile Ser Glu Phe 
        1235                1240                1245            
Ala Lys Arg Tyr Thr Leu Ala Asp Ala Asn Leu Glu Lys Ile Asn Thr 
    1250                1255                1260                
Leu Phe Glu Gln Asn Lys Glu Gly Asp Ile Lys Val Ile Ala Gln Ser 
1265                1270                1275                1280
Phe Val Asn Leu Met Ala Phe Asn Ala Met Gly Ala Pro Ala Ser Phe 
                1285                1290                1295    
Lys Phe Phe Glu Thr Thr Ile Glu Arg Lys Arg Tyr Asn Asn Leu Lys 
            1300                1305                1310        
Glu Leu Leu Asn Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Ala Arg Lys Arg Leu Asp Asp Leu Gly Glu Asp 
    1330                1335                1340

<210> 94
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #91

<400> 94
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Gln Phe Val Glu Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Glu Glu Ser His Leu Ser Glu Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys Leu Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Ala Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Arg Gly Lys Thr Arg Phe Phe Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Lys Asp Tyr Leu Lys Lys Glu Phe Glu Glu Phe Asp Ser 
                565                 570                 575     
Val Asp Ile Thr Gly Ile Glu Asp Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu Lys 
705                 710                 715                 720 
Glu Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Glu Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys Lys Tyr Asn Asp Arg Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Lys Lys Gly Tyr Gln Asn Ile Gln 
        1155                1160                1165            
Glu Asn Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Asp Lys Ser Asn Asn Ser Glu Asn Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Asp Glu Leu Leu Asp Tyr Ile Ile Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Val Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 95
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #92

<400> 95
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe His Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asp Pro Leu Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Leu Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Ser Asp Glu Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Phe Leu Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asn Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     
Val Asp Ile Thr Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala Asp 
625                 630                 635                 640 
Leu Phe Asp Lys Asn Val Leu Lys Lys Leu Lys Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Gln Thr Asp Ser Leu Lys 
705                 710                 715                 720 
Glu Val Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Thr Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Gln Gly Ile Lys Asn Ser Arg Gln Arg Met Lys Arg Leu Glu Glu 
    770                 775                 780                 
Val Leu Lys Lys Leu Gly Ser Asn Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Thr Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Thr Ser Ser Glu Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys Ser Tyr 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ser Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Lys Leu 
945                 950                 955                 960 
Val Ser Asp Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys Lys Tyr Asp Asp Lys Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asn Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Lys Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Ala Lys Gly Tyr Gln Asp Ile Gln 
        1155                1160                1165            
Glu Asn Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Phe Leu Tyr His Ala 
                1205                1210                1215    
Ser Arg Ile Asp Lys Ser Thr Ser Ser Glu Asn Leu Glu Tyr Val Glu 
            1220                1225                1230        
Gln His Arg His Glu Phe Asp Glu Ile Leu Asp Tyr Ile Ile Asp Phe 
        1235                1240                1245            
Ser Glu Arg Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Asn 
    1250                1255                1260                
Leu Tyr Asn Glu Asn Asp Asp Ser Asp Ile Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Ala Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Val Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp 
    1330                1335                1340

<210> 96
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #93

<400> 96
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe His Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asp Pro Leu Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Leu Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Asn Asp Glu Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Glu Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asn Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Glu Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Phe Leu Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     
Val Asp Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Glu Lys Arg Leu Ala Thr Tyr Ala Asp 
625                 630                 635                 640 
Leu Phe Asp Lys Asn Val Leu Lys Gln Leu Lys Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ser Gly Gln Thr Asp Ser Leu Lys 
705                 710                 715                 720 
Glu Gln Ile Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Thr Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Gln Gly Ile Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu Glu 
    770                 775                 780                 
Val Leu Lys Lys Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val Glu 
785                 790                 795                 800 
Asn Thr Gln Leu Gln Asn Glu Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asn Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Glu Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Lys Lys Met Lys Ser Tyr 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ser Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Pro Ile Arg Asn Val Lys Val Ile Thr Leu Lys Ser Lys Leu 
945                 950                 955                 960 
Val Ser Asp Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys Lys Tyr Asp Asp Lys Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asn Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Met Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Lys Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Ala Lys Gly Tyr Gln Asp Ile Gln 
        1155                1160                1165            
Glu Asn Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Thr Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Phe Leu Tyr His Ala 
                1205                1210                1215    
Ser Lys Ile Asp Lys Ser Thr Ser Ser Glu Asn Leu Glu Tyr Val Glu 
            1220                1225                1230        
Gln His Arg His Glu Phe Asp Glu Ile Leu Asp Tyr Ile Ile Asp Phe 
        1235                1240                1245            
Ser Glu Arg Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Asn 
    1250                1255                1260                
Leu Phe Asn Glu Asn Asp Asp Ser Asp Ile Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Leu Asp Ala Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Val Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp 
    1330                1335                1340

<210> 97
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #94

<400> 97
Met Glu Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Ser Asp Val Asp Lys Leu Phe Tyr Gln Leu Val Gln Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Leu Asp Ala Ser Gly Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Leu Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Ala Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Glu Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Glu Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Phe Leu Ser Gly Glu Gln Lys 
    530                 535                 540                 
Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     
Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala His 
625                 630                 635                 640 
Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu His 
705                 710                 715                 720 
Glu Gln Ile Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Thr Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Lys Gly Leu Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu Glu 
    770                 775                 780                 
Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val Glu 
785                 790                 795                 800 
Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu Ser 
            820                 825                 830         
Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn Tyr 
865                 870                 875                 880 
Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ser Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Pro Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys Leu 
945                 950                 955                 960 
Val Ser Asp Phe Arg Lys Asp Phe Gln Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys Val Tyr Asp Glu Gln Glu Ile Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Arg Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asn Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Met Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Arg Asn Ser Asp Lys Leu Ile Pro Arg Lys Lys Asp Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Lys Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Ser 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Asp Phe Leu Glu Ala Lys Gly Tyr Lys Asp Ile Gln 
        1155                1160                1165            
Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Thr Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Phe Leu Tyr His Ala 
                1205                1210                1215    
Ser Lys Ile Glu Lys Ser Thr Ser Ser Glu Asn Leu Ala Tyr Val Glu 
            1220                1225                1230        
Gln His Arg His Glu Phe Asp Glu Ile Leu Glu Tyr Ile Ile Asp Phe 
        1235                1240                1245            
Ser Glu Arg Tyr Ile Leu Ala Asp Lys Asn Leu Ser Lys Val Lys Ser 
    1250                1255                1260                
Leu Phe Asn Glu His Glu Asp Ser Asp Ile Ser Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Leu Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp 
    1330                1335                1340

<210> 98
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #95

<400> 98
Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Phe Leu Ser Gly Glu Gln Lys 
    530                 535                 540                 
Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     
Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala His 
625                 630                 635                 640 
Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu His 
705                 710                 715                 720 
Glu Gln Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu Glu 
    770                 775                 780                 
Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val Glu 
785                 790                 795                 800 
Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu Ser 
            820                 825                 830         
Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn Tyr 
865                 870                 875                 880 
Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu Asn 
    930                 935                 940                 
Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys Leu 
945                 950                 955                 960 
Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys Val Tyr Asp Glu Gln Glu Ile Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Arg Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile 
1025                1030                1035                1040
Thr Leu Ala Asn Glu Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Met Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Arg Asn Ser Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Lys Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Ser 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Asp Phe Leu Glu Ala Lys Gly Tyr Lys Asp Val Gln 
        1155                1160                1165            
Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala Lys Leu Val Thr Phe Leu Tyr His Ala 
                1205                1210                1215    
Ser Lys Ile Glu Lys Ser Lys Ser Pro Glu Asn Asn Ala Tyr Val Glu 
            1220                1225                1230        
Gln His Arg His Asp Leu Asp Glu Ile Leu Glu Tyr Ile Ser Glu Phe 
        1235                1240                1245            
Ser Lys Arg Tyr Ile Leu Ala Asp Lys Asn Leu Ser Lys Val Lys Ser 
    1250                1255                1260                
Leu Phe Asn Lys His Glu Asp Ser Ser Ile Ser Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Ile Ile Asn Leu Phe Thr Leu Thr Ser Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Leu Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp 
    1330                1335                1340

<210> 99
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #96

<400> 99
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Glu Glu Ser Phe Leu Val Pro Glu Asp Lys Glu 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Ser Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe His Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Leu Leu Phe Glu Glu Asp Pro Leu Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Leu Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gly Leu Ser Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Ser Asp Glu Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Lys Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Glu Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Phe Leu Ser Ala Glu Gln Lys 
    530                 535                 540                 
Gln Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asn Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     
Val Asp Ile Thr Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             
Glu Glu Asn Gln Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Lys Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala Asp 
625                 630                 635                 640 
Leu Phe Asp Lys Ser Val Leu Lys Lys Leu Lys Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Ser Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Lys Thr Glu Ser Leu Lys 
705                 710                 715                 720 
Glu Val Val Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Thr Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ala Gln Gly Ile Lys Asn Ser Arg Gln Arg Met Lys Lys Leu Glu Glu 
    770                 775                 780                 
Val Leu Lys Lys Leu Gly Ser Asn Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Thr Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Asp Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Thr Ser Gln Glu Ala Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys Ser Tyr 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Lys Ala Gly Ala Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ser Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Leu Asp Glu Asn 
    930                 935                 940                 
Asn Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Lys Leu 
945                 950                 955                 960 
Val Ser Asp Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys Lys Tyr Asp Asp Lys Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asn Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Ile Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Lys Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Ala Lys Gly Tyr Gln Asp Ile Gln 
        1155                1160                1165            
Glu Asn Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Leu Val Leu Pro Ala His Tyr Val Thr Phe Leu Tyr Leu Ala 
                1205                1210                1215    
Ser Arg Tyr Asp Lys Leu Thr Ser Ser Glu Asp Leu His Phe Val Glu 
            1220                1225                1230        
Gln His Leu His Tyr Phe Asp Glu Ile Leu Asp Ile Ile Ile Asp Phe 
        1235                1240                1245            
Ser Glu Arg Tyr Ile Leu Ala Asp Ser Asn Leu Glu Lys Ile Lys Asn 
    1250                1255                1260                
Leu Tyr Asn Glu Asn Asp Asp Phe Ser Ile Asn Glu Gln Ala Ser Asn 
1265                1270                1275                1280
Met Ile Asn Leu Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Ala Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Val Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp 
    1330                1335                1340

<210> 100
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #97

<400> 100
Met Arg Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Asn Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ala 
                85                  90                  95      
Phe Phe Gln Arg Leu Ser Glu Ser Phe Leu Val Pro Glu Asp Lys Glu 
            100                 105                 110         
Phe Asp Arg His Pro Ile Phe Gly Asn Leu Lys Asp Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Asp Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Leu Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Leu Leu Phe Glu Glu Asp Pro Ile Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Thr Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Glu Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gly Leu Ser Glu Asp Ala Lys Leu Gln Ile Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Ser Asp Glu Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Lys Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asn Lys Glu Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Lys Glu Leu His Ala Ile Leu Arg Arg Gln Glu Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Ser Phe Ala Trp 
    450                 455                 460                 
Leu Lys Arg Arg Ser Asp Glu Ser Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Glu Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Pro Leu Tyr Glu Met Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Phe Leu Ser Ala Glu Gln Lys 
    530                 535                 540                 
Glu Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asn Tyr Phe Ser Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     
Val Asp Ile Thr Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Ala Phe Leu Asp Asp 
        595                 600                 605             
Glu Ala Asn Gln Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Glu Lys Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala Asp 
625                 630                 635                 640 
Leu Phe Asp Lys Ser Val Leu Lys Lys Leu Lys Lys Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Ser Leu Asp Phe Glu 
    690                 695                 700                 
Lys Ile Ile Gln Asn Ala Gln Glu Lys Ala Lys Ser Glu Ser Leu Lys 
705                 710                 715                 720 
Glu Thr Val Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Ile Lys Ile Val Asp Glu Ile Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ala Gln Gly Ile Lys Asn Ser Arg Gln Arg Leu Lys Lys Leu Glu Glu 
    770                 775                 780                 
Val His Lys Lys Thr Gly Ser Lys Ile Leu Lys Glu His Ser Val Ser 
785                 790                 795                 800 
Asn Thr Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Leu Leu Gln Asp 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Gln Asp Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Ile Val Leu Thr Ser Gln Glu Ala Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys Ser Tyr 
865                 870                 875                 880 
Trp Gln Lys Gln Leu Lys Ser Gly Ala Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
His Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ser Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Ser Lys Leu Asp Glu Asn 
    930                 935                 940                 
Asn Lys Ser Asn Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Lys Met 
945                 950                 955                 960 
Val Ser Asp Phe Arg Lys Asp Phe Gly Leu Tyr Lys Leu Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Ala Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys His Tyr Asp Asp Ser Ser Leu Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Lys Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Val Lys Asp 
                1045                1050                1055    
Phe Lys Thr Ile Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Ile Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Lys Val Ala Lys Gly Lys Ala Gln Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Gln Ser Glu Phe Glu 
            1140                1145                1150        
Lys Asp Pro Ile Ala Phe Leu Glu Lys Lys Gly Tyr Gln Asp Ile Gln 
        1155                1160                1165            
Glu Asn Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Leu Ala Leu Pro Ala Lys Tyr Val Lys Phe Leu Tyr Leu Ala 
                1205                1210                1215    
Ser Arg Tyr Asp Lys Leu Thr Lys Ser Glu Asp Arg His Phe Val Glu 
            1220                1225                1230        
Gln His Leu His Tyr Phe Asp Glu Ile Leu Asp Ile Ile Val Asp Phe 
        1235                1240                1245            
Ser Asn Arg Tyr Ile Leu Ala Asp Ser Asn Leu Glu Lys Ile Lys Asn 
    1250                1255                1260                
Leu Tyr Asn Glu Lys Asp Asp Phe Ser Ile Asn Glu Gln Ala Ser Asn 
1265                1270                1275                1280
Met Leu Asn Leu Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Ala Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Asn 
            1300                1305                1310        
Glu Val Leu Asn Ser Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Gly Asp 
    1330                1335                1340

<210> 101
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #98

<400> 101
Met Arg Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Lys 
65                  70                  75                  80  
Tyr Leu Gln Asn Ile Phe Ser Asn Glu Met Ser Lys Val Asp Ser Ala 
                85                  90                  95      
Phe Phe Gln Arg Leu Ser Glu Ser Phe Leu Val Pro Asp Asp Lys Glu 
            100                 105                 110         
Phe Asp Arg His Pro Ile Phe Gly Asn Leu Lys Asp Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Asp Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asp Ser 
                165                 170                 175     
Glu Asn Thr Asp Val His Ala Leu Phe Leu Lys Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Leu Leu Phe Glu Glu Asp Pro Ile Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Thr Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Glu Ile Pro Asn Gln Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ser Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Glu Leu Ser Glu Asp Ala Lys Leu Gln Ile Ser Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Ile Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Lys Gly Ala Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Val Gln Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Lys Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Asp 
            340                 345                 350         
Asn Lys Glu Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Lys Leu Ile Ser Lys Leu Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Asn Glu Leu Lys Ala Ile Ile Arg Arg Gln Glu Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Leu Phe Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Ser Phe Ala Trp 
    450                 455                 460                 
Leu Lys Arg Arg Ser Asn Glu Ser Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Gln Glu Ala Ser Ala Arg Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Pro Leu Tyr Glu Met Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Gln Thr Glu Gly Met Lys Lys Pro Phe Leu Ser Ser Glu Glu Lys 
    530                 535                 540                 
Glu Glu Ile Val Asn Leu Leu Phe Lys Lys Glu Arg Lys Val Thr Val 
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asn Tyr Phe Ser Lys Ile Lys Cys Phe Asp Ser 
                565                 570                 575     
Val Thr Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Ala Phe Leu Asp Asp 
        595                 600                 605             
Glu Ala Asn Gln Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Glu Lys Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala Asp 
625                 630                 635                 640 
Val Phe Asp Lys Ser Val Leu Lys Lys Leu Lys Lys Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Gln Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Thr Gly Lys Thr Ile Leu Asp Phe Leu Lys Asp Asp Gly Val Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Ser Leu Asp Phe Ala 
    690                 695                 700                 
Lys Ile Ile Lys Asn Ala Gln Glu Lys Thr Lys Ser Glu Ser Leu Glu 
705                 710                 715                 720 
Glu Thr Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Ile Lys Ile Val Asp Glu Ile Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Ser Thr 
        755                 760                 765             
Ala Gln Gly Ile Lys Asn Ser Arg Gln Arg Leu Arg Lys Leu Glu Glu 
    770                 775                 780                 
Val His Lys Asn Thr Gly Ser Lys Ile Leu Lys Glu His Asn Val Ser 
785                 790                 795                 800 
Asn Thr Gln Leu Gln Ser Asp Arg Leu Tyr Leu Tyr Leu Leu Gln Asp 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Lys Asp Leu Asp Phe Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Ile Val Leu Thr Ser Gln Glu Ser Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Ile Val Asn Lys Met Lys Ser Tyr 
865                 870                 875                 880 
Trp Gln Lys Gln Leu Lys Ser Gly Ala Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
His Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Phe Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Ser Asn Leu Asp Glu Asn 
    930                 935                 940                 
Ser Lys Ser Asn Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Lys Met 
945                 950                 955                 960 
Val Ser Asp Phe Arg Lys Asp Phe Gly Phe Tyr Lys Leu Arg Glu Val 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Ala Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys His Tyr Asp Asp Ser Ser Leu Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Lys Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Val Lys Asp 
                1045                1050                1055    
Ile Lys Thr Ile Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Asn Asn Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Ile Ile Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Lys Val Ala Lys Gly Lys Ala Gln Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Gln Asn Glu Phe Glu 
            1140                1145                1150        
Lys Asp Pro Ile Ala Phe Leu Glu Lys Lys Gly Tyr Gln Asp Ile Gln 
        1155                1160                1165            
Glu Ser Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Lys Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Leu Ala Leu Pro Asn Lys Tyr Val Lys Phe Leu Tyr Leu Ala 
                1205                1210                1215    
Ser Arg Tyr Thr Lys Phe Thr Lys Glu Glu Asp Arg His Phe Val Glu 
            1220                1225                1230        
Ser His Leu His Tyr Phe Asp Glu Ile Met Asp Ile Ile Val Asp Phe 
        1235                1240                1245            
Ser Asn Arg Tyr Ile Leu Ala Asp Ser Asn Leu Glu Lys Ile Gln Asn 
    1250                1255                1260                
Leu Tyr Asn Glu Lys Asp Asn Phe Ser Ile Glu Glu Gln Ala Thr Asn 
1265                1270                1275                1280
Met Leu Asn Leu Phe Thr Phe Thr Gly Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asn Ala Asp Ile Asp Arg Lys Arg Tyr Thr Ser Thr Asn 
            1300                1305                1310        
Glu Ile Leu Asn Ser Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Gly Asp 
    1330                1335                1340

<210> 102
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #99

<400> 102
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Glu Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Gln Thr Phe Glu Glu Ser His Leu Ser Glu Gln Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys Leu Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu Asn Leu Pro Asp Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Lys Thr Arg Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Leu Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Leu Asp Tyr Leu Lys Lys Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu Lys 
705                 710                 715                 720 
Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Asp 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Lys Lys Tyr Asn Tyr Arg Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Lys Lys Gly Tyr Gln Asn Ile Gln 
        1155                1160                1165            
Glu Asn Lys Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Asp Lys Ser Asn Asn Pro Glu His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Val Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 103
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #100

<400> 103
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Ile Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Gln Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Glu Ser His Leu Ser Glu Ile Thr Ile Asp Ala 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Glu Asn Val Pro Asp Gln Tyr Asn Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Thr Tyr Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Ser Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ala Lys Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Glu Lys Leu Leu Asn Tyr Leu Lys Lys Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             
Glu Ala Asn Glu Asp Ile Ile Glu Asp Ile Ile Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Val Asp Asn Leu Ala 
705                 710                 715                 720 
Gln Val Val His Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Gln Asp 
    770                 775                 780                 
Ser Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Lys Lys Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Ala Arg Lys Ser Phe 
865                 870                 875                 880 
Trp Val Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Val Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Lys Lys Tyr Asn Tyr Arg Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Gln Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Lys Lys Gly Tyr His Asn Ile Gln 
        1155                1160                1165            
Glu Asp Lys Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Asp Lys Ser Asn Asn Pro Glu His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Val Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ala 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asp Asp Ala Asp Ile Glu Glu Ile Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Gly Glu Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Cys Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 104
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #101

<400> 104
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Val Asp Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Ile Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Gln Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Glu Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Val Asn Ala Pro Asp Gln Tyr Asn Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ala Lys Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             
Lys Ala Asn Glu Gln Ile Ile Glu Asp Ile Ile Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Asp Val Asp Asp Ile Ala 
705                 710                 715                 720 
Gln Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Gln Asp 
    770                 775                 780                 
Ser Leu Lys Asn Leu Gly Ser Asn Ile Asn Lys Lys Lys Pro Val Glu 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Ala Arg Lys Ser Phe 
865                 870                 875                 880 
Trp Val Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Val Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Lys Lys Tyr Asn Tyr Arg Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asn Lys Gln Lys Asp 
                1045                1050                1055    
Phe Glu Ile Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Lys Lys Gly Tyr His Asn Ile Gln 
        1155                1160                1165            
Glu Asp Lys Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Asp Lys Ser Asn Asn Pro Glu His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Val Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ala 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asp Asp Ala Ser Ile Glu Glu Ile Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Asn Phe Phe Gly Glu Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Cys Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 105
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #102

<400> 105
Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Asp Glu Asp Lys Arg 
            100                 105                 110         
Gly Glu Arg His Pro Ile Phe Gly Asn Ile Ala Ala Glu Val Lys Tyr 
        115                 120                 125             
His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Thr Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Lys Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Ala Leu Phe Lys Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Lys Thr Ile Glu Glu Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Leu Ser Ile Leu Thr Glu Lys Val Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Gly Leu Leu Gly Glu Val Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Ala Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Val Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Lys Lys Leu Lys 
                325                 330                 335     
Asp Phe Ile Lys Val Asn Ala Pro Asp Gln Tyr Asn Ala Ile Phe Lys 
            340                 345                 350         
Asp Lys Asn Lys Lys Gly Tyr Ala Gly Tyr Ile Glu Ser Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Leu Lys Ile Asn 
    370                 375                 380                 
Gly Ser Gly Asp Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Lys Ala Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Ile Leu Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Glu Ala Lys Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Asp Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     
Val Asn Leu Thr Gly Leu Asp Lys Glu Phe Asn Ser Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Arg Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             
Lys Ala Asn Glu Gln Ile Ile Glu Asp Ile Ile Gln Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Gln Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Lys Ala Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Asn Lys Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Tyr Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Ala Lys Ala Gln Ile Ile Gly Asp Val Asp Asp Ile Ala 
705                 710                 715                 720 
Asn Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Ala Asn Ile Ile Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asp Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Leu Leu Gln Asp 
    770                 775                 780                 
Ser Leu Lys Asn Leu Asp Asn Pro Val Asn Ile Lys Asn Pro Val Glu 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asn Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Ala Arg Lys Ser Phe 
865                 870                 875                 880 
Trp Val Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu His Asp Glu Asn 
    930                 935                 940                 
Lys Lys Val Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Lys Lys Tyr Asn Tyr Arg Glu Arg Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Lys Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Asp Gly Glu Ile Ala Trp Asn Lys Gln Lys Asp 
                1045                1050                1055    
Phe Lys Ile Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Ser Tyr Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ser Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Gln Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Lys Lys Gly Tyr Arg Asn Ile Arg 
        1155                1160                1165            
Glu Asp Lys Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Leu Val Ile Pro Gln Arg Phe Thr Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Asp Lys Ser Asn Asn Pro Glu His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asp Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Val Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ala 
    1250                1255                1260                
Leu Phe Ala Glu Asn Asp Asp Ala Ser Ile Glu Glu Ile Ala Thr Ser 
1265                1270                1275                1280
Phe Ile Ser Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Asn Phe Phe Gly Glu Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Cys Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 106
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #103

<400> 106
Met Lys Lys Ser Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Val Asn Lys Ser Glu Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Arg Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ser Ser Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     
Lys Asp Leu Ile Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             
Lys Thr Asn Glu Gln Ile Ile Glu Asp Ile Ile Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Lys Gln Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Gln Ile Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp Ile Glu 
705                 710                 715                 720 
Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Asp 
            740                 745                 750         
Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Arg Ser Lys Ser Gln Gln Arg Leu Lys Arg Leu Gln Asp 
    770                 775                 780                 
Ser Leu Lys Glu Leu Gly Ser Asn Leu Asn Lys Lys Lys Pro Val Glu 
785                 790                 795                 800 
Asn Ser His Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp His Leu Ser 
            820                 825                 830         
Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Ile Glu Ile Val Arg Ala Arg Lys Ser Tyr 
865                 870                 875                 880 
Trp Val Lys Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Lys Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Lys Lys Tyr Asp His Ser Glu Met Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Tyr Ala Asp Asp Thr Gly Glu Ile Val Trp Asn Lys Gln Lys Asp 
                1045                1050                1055    
Phe Glu Ile Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Val Tyr Trp Asp 
    1090                1095                1100                
Thr Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Ser Ile Met Glu Arg Ser Phe Phe Glu 
            1140                1145                1150        
Glu Asn Pro Val Ala Phe Leu Glu Lys Lys Gly Tyr His Asn Val Gln 
        1155                1160                1165            
Glu Asp Lys Leu Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Phe Glu 
    1170                1175                1180                
Gly Gly Arg Arg Arg Leu Leu Ala Ser Ala Thr Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Glu Leu Leu Tyr His Ala 
                1205                1210                1215    
His Arg Ile Asp Asn Phe Asn Ser Thr Glu His Leu Lys Tyr Val Ser 
            1220                1225                1230        
Glu His Lys Lys Glu Phe Glu Glu Val Leu Ser Cys Val Glu Asn Phe 
        1235                1240                1245            
Ala Asn Leu Tyr Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala 
    1250                1255                1260                
Ala Ala Glu Ser Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Asn Phe Leu Gly Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Cys Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Glu 
    1330                1335                1340

<210> 107
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #104

<400> 107
Met Glu Lys Ser Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Lys Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Glu Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Pro Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys Gln Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Val Asn Lys Leu Glu Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Ser Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Ala Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Lys Tyr His Ser Asp Glu Pro Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ser Phe Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Ala Lys Leu Leu Ser Tyr Leu Asn Asn Glu Phe Glu Glu Phe Arg Ile 
                565                 570                 575     
Asn Asp Leu Ile Gly Leu Asp Lys Asp Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             
Lys Thr Asn Glu Gln Ile Ile Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Ser Gln Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Asn Asn Lys Thr Ile Leu Asp Phe Leu Ile Asp Asp Gly His Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe Lys 
    690                 695                 700                 
Thr Ile Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp Ile Glu 
705                 710                 715                 720 
Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Asp 
            740                 745                 750         
Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Gly Tyr Gly Arg Asn Lys Ser Asn Gln Arg Leu Lys Arg Leu Gln Asp 
    770                 775                 780                 
Ser Leu Lys Glu Phe Gly Ser Asp Leu Ser Lys Lys Lys Pro Val Glu 
785                 790                 795                 800 
Asn Ser His Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp His Leu Ser 
            820                 825                 830         
Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Ile Glu Ile Val Arg Asn Arg Lys Ser Tyr 
865                 870                 875                 880 
Trp Tyr Lys Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Lys Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Lys Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Lys Lys Tyr Asp His Ser Glu Met Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Tyr Ala Asp Ala Asp Gly Glu Val Val Trp Asn Lys Gln Lys Asp 
                1045                1050                1055    
Phe Asp Ile Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Val Tyr Trp Asp 
    1090                1095                1100                
Thr Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Ser Ile Met Glu Arg Ser Phe Phe Glu 
            1140                1145                1150        
Glu Asn Pro Val Ser Phe Leu Glu Lys Lys Gly Tyr His Asn Val Gln 
        1155                1160                1165            
Glu Asp Lys Leu Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Phe Glu 
    1170                1175                1180                
Gly Gly Arg Arg Arg Leu Leu Ala Ser Ala Thr Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Val Val Leu Pro Ala His Leu Val Glu Leu Leu Tyr His Ala 
                1205                1210                1215    
His Arg Ile Asp Asn Phe Asn Ser Thr Glu His Leu Lys Tyr Val Ser 
            1220                1225                1230        
Glu His Lys Lys Glu Phe Glu Glu Val Leu Ser Cys Val Glu Asn Phe 
        1235                1240                1245            
Ala Asn Leu Tyr Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala 
    1250                1255                1260                
Ala Ala Glu Ser Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Ala Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Asn Phe Leu Gly Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Cys Leu Ser Ala Thr Leu Ile His Gln Ser Val Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Glu 
    1330                1335                1340

<210> 108
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #105

<400> 108
Thr Lys Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Ala Asn Lys Ser Glu Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Arg Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Lys 
465                 470                 475                 480 
Val Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Asp Ser Phe Phe Asp Ser Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu Tyr Arg Ile 
                565                 570                 575     
Lys Asp Leu Ile Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp Asp 
        595                 600                 605             
Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile Lys Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Lys Gln Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Gln Ile Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp Ile Glu 
705                 710                 715                 720 
Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Asp 
            740                 745                 750         
Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu Lys Lys Leu Gln Asn 
    770                 775                 780                 
Ser Leu Lys Glu Leu Gly Ser Asn Leu Asn Glu Glu Lys Pro Val Glu 
785                 790                 795                 800 
Asn Ser His Leu Gln Asn Asp Gln Leu Phe Leu Tyr Tyr Ile Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Asp Glu Leu Asp Ile Asp His Leu Ser 
            820                 825                 830         
Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Leu Asp Ile Val Arg Ala Arg Lys Ala Glu 
865                 870                 875                 880 
Trp Val Arg Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Lys Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Lys Lys Tyr Asp His Ser Glu Met Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Thr Val Val 
1025                1030                1035                1040
Lys Tyr Ala Asp Asp Thr Gly Glu Ile Ala Trp Asn Lys Gln Thr Asp 
                1045                1050                1055    
Phe Glu Lys Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Thr Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Val Tyr Trp Asp 
    1090                1095                1100                
Thr Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Ser Ile Met Glu Arg Ser Phe Phe Glu 
            1140                1145                1150        
Glu Asn Pro Val Glu Phe Leu Glu Asn Lys Gly Tyr His Asn Val Gln 
        1155                1160                1165            
Glu Asp Lys Leu Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Phe Glu 
    1170                1175                1180                
Gly Gly Arg Arg Arg Leu Leu Ala Ser Ala Thr Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Glu Leu Leu Tyr His Ala 
                1205                1210                1215    
His Arg Ile Asp Asn Phe Asn Ser Thr Glu His Leu Lys Tyr Val Ser 
            1220                1225                1230        
Glu His Lys Lys Glu Phe Glu Glu Val Leu Ser Cys Val Glu Asn Phe 
        1235                1240                1245            
Ala Asn Leu Tyr Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala 
    1250                1255                1260                
Ala Ala Asp Ser Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Asn Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Asn Phe Leu Gly Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Cys Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Glu 
    1330                1335                1340

<210> 109
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #106

<400> 109
Thr Lys Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Ile Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys Tyr Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Ala Asn Lys Ser Glu Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Asn Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Arg Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Lys 
465                 470                 475                 480 
Val Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Tyr Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Asp Ser Phe Phe Asp Ser Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu Tyr Arg Ile 
                565                 570                 575     
Lys Asp Leu Ile Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ala Phe Leu Asp Asp 
        595                 600                 605             
Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile Lys Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Lys Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Ala Asn Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ala 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Pro Phe Lys 
    690                 695                 700                 
Gln Ile Ile Gln Lys Ser Gln Val Val Gly Asp Val Asp Asp Ile Glu 
705                 710                 715                 720 
Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Asp 
            740                 745                 750         
Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu Lys Lys Leu Gln Asn 
    770                 775                 780                 
Ser Leu Lys Glu Leu Gly Ser Asn Leu Asn Glu Glu Lys Pro Val Glu 
785                 790                 795                 800 
Asn Ser His Leu Gln Asn Asp Gln Leu Phe Leu Tyr Tyr Ile Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Asp Glu Leu Asp Ile Asp His Leu Ser 
            820                 825                 830         
Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Leu Asp Ile Val Arg Ala Arg Lys Ala Glu 
865                 870                 875                 880 
Trp Val Arg Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp Glu Asn 
    930                 935                 940                 
Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Asp Phe Glu Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Ser Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Lys Lys Tyr Asp His Ser Glu Met Gly Lys Ala Thr Ala 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Met Asn Phe Phe Lys Thr Val Val 
1025                1030                1035                1040
Lys Tyr Ala Asp Asp Thr Gly Glu Ile Ala Trp Asn Lys Gln Thr Asp 
                1045                1050                1055    
Phe Glu Lys Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Thr Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asp Ser Asp Lys Leu Ile Pro Arg Lys Val Tyr Trp Asp 
    1090                1095                1100                
Thr Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Phe Val Val Ala Asp Val Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Ser Ile Met Glu Arg Ser Phe Phe Glu 
            1140                1145                1150        
Glu Asn Pro Val Glu Phe Leu Glu Asn Lys Gly Tyr His Asn Ile Arg 
        1155                1160                1165            
Glu Asp Lys Leu Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Phe Glu 
    1170                1175                1180                
Gly Gly Arg Arg Arg Leu Leu Ala Ser Ala Ser Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Gly His Leu Val Glu Leu Leu Tyr His Ala 
                1205                1210                1215    
His Arg Ile Asp Asn Phe Asn Ser Thr Glu Tyr Leu Asn Tyr Val Ser 
            1220                1225                1230        
Glu His Lys Lys Glu Phe Glu Lys Val Leu Ser Cys Val Glu Asp Phe 
        1235                1240                1245            
Ala Asn Leu Tyr Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala 
    1250                1255                1260                
Val Ala Asp Ser Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Asn Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe 
                1285                1290                1295    
Asn Phe Leu Gly Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys 
            1300                1305                1310        
Glu Cys Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Glu 
    1330                1335                1340

<210> 110
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #107

<400> 110
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Glu Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Leu Leu Lys Leu Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Gln Asn Leu Pro Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Lys Thr Arg Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp His Leu Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Leu Asp Tyr Leu Glu Lys Glu Phe Asp Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu Lys 
705                 710                 715                 720 
Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Asp 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Ala Arg Phe Asn Thr Glu Leu Asp Glu Asn 
    930                 935                 940                 
Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Pro Lys Tyr Asn Tyr Arg Glu Arg Lys Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Arg Lys Gly Tyr Gln Asn Ile Gln 
        1155                1160                1165            
Glu Asn Lys Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile His Lys Val Asn Glu Pro Glu His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Gly Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Val Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 111
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #108

<400> 111
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Glu Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Leu Leu Lys Leu Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asp Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Gln Asn Leu Pro Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Ala Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Arg Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Lys Thr Arg Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Gly Leu Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Leu Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Tyr Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp Asp 
        595                 600                 605             
Ser Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Leu Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu Lys 
705                 710                 715                 720 
Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Asp 
    770                 775                 780                 
Ala Ile Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Arg Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Asp Val Val Arg Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Leu Asp Glu Asn 
    930                 935                 940                 
Asp Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Lys Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Pro Lys Tyr Asn Tyr Arg Glu Arg Lys Lys Ala Thr Ala 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys His 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Trp Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ser Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Arg Lys Gly Tyr Gln Asn Ile Gln 
        1155                1160                1165            
Glu Asn Lys Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile His Lys Val Asn Glu Pro Glu His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Val Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 112
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #109

<400> 112
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Val Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Ser His Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Gly Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Thr Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Glu Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Val Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Leu Leu Lys Leu Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Glu Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Glu Leu Glu Val Leu Leu Ala Gln Ile Gly Asp Asn Tyr Ala Glu 
        275                 280                 285             
Leu Phe Leu Ser Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Val Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asn Glu His Gln Met Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Gln Lys Leu Ser Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Val Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
        355                 360                 365             
Gln Glu Ala Phe Tyr Lys Tyr Leu Lys Gly Leu Leu Asn Lys Ile Glu 
    370                 375                 380                 
Gly Ser Gly Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Arg Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro Phe 
            420                 425                 430         
Leu Ala Asp Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Ala Asp Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Ile Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asn Gln Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Lys Thr Glu Gln Gly Lys Thr Ala Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr Lys 
545                 550                 555                 560 
Asp Lys Leu Met Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Tyr Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Arg Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             
Ser Lys Asn Glu Lys Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Asn Tyr Ser Asp 
625                 630                 635                 640 
Leu Leu Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Glu Leu Ile His Gly Ile Arg Asn Lys 
            660                 665                 670         
Glu Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Asn Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu Asn 
705                 710                 715                 720 
Gln Val Val Ser Asp Ile Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Ile Met Gly His 
            740                 745                 750         
Gln Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Phe Thr 
        755                 760                 765             
Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Gly Leu Thr Asp 
    770                 775                 780                 
Ser Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro Val Glu 
785                 790                 795                 800 
Asn Ser Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Arg Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Tyr Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Lys Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Lys Asp Val Val Arg Lys Met Lys Ser Tyr 
865                 870                 875                 880 
Trp Ser Lys Leu Leu Ser Ala Lys Leu Ile Thr Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Thr Asp Glu Asn 
    930                 935                 940                 
Asn Lys Lys Ile Arg Gln Val Lys Ile Val Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Ile Gly 
            980                 985                 990         
Lys Ala Leu Leu Gly Val Tyr Pro Gln Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Pro His Phe His Gly His Lys Glu Asn Lys Ala Thr Ala 
    1010                1015                1020                
Lys Lys Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Lys Asp Asp 
1025                1030                1035                1040
Val Arg Thr Asp Lys Asn Gly Glu Ile Ile Trp Lys Lys Asp Glu His 
                1045                1050                1055    
Ile Ser Asn Ile Lys Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Phe Tyr Trp Asp 
    1090                1095                1100                
Thr Lys Lys Tyr Gly Gly Phe Asp Ser Pro Ile Val Ala Tyr Ser Ile 
1105                1110                1115                1120
Leu Val Ile Ala Asp Ile Glu Lys Gly Lys Ser Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Asp Leu Val Gly Ile Thr Ile Met Glu Arg Thr Thr Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Arg Lys Gly Tyr Arg Asn Val Gln 
        1155                1160                1165            
Glu Glu Asn Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Arg Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Ile Val Leu Pro Asn His Leu Gly Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Asn Ile His Lys Val Asp Glu Pro Lys His Leu Asp Tyr Val Glu 
            1220                1225                1230        
Lys His Lys Asp Glu Phe Lys Glu Leu Leu Asp Val Val Ser Asn Phe 
        1235                1240                1245            
Ser Lys Lys Tyr Ile Leu Ala Glu Ser Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Leu Tyr Ala Gln Asn Asn Asn Glu Asp Ile Lys Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Ile Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Ile Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Gly Asp 
    1330                1335                1340

<210> 113
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #110

<400> 113
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Leu Asp Val 
                165                 170                 175     
Arg Asn Thr Asp Val Gln Lys Leu Phe Gln Glu Phe Leu Glu Val Tyr 
            180                 185                 190         
Asp Ser Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Lys Leu Glu Arg 
    210                 215                 220                 
Leu Leu Ala Gln Phe Pro Asn Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asp Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Ala Asn Leu Pro Asp Lys Tyr Lys Glu Val Phe Ser 
            340                 345                 350         
Asp Ala Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Ser Lys Val Glu 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His Leu 
                405                 410                 415     
Ala Glu Met Lys Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Asn Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Glu Thr Arg Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Gly Leu Phe Lys Glu Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Leu Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Ile Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Tyr Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp Asp 
        595                 600                 605             
Ser Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Leu Phe Thr Lys Glu Gln Leu Lys Lys Leu Ser Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Arg Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Asp Ser Asp Asn Leu Lys 
705                 710                 715                 720 
Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         
Asn Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Ile Lys Asn Leu Gly Ser Asp Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Arg Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Asp Val Val Lys Lys Met Lys Ser Phe 
865                 870                 875                 880 
Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Ala Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Leu Asp Glu Asn 
    930                 935                 940                 
Asp Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Asn Phe Arg Lys Glu Phe Glu Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Lys Ala Leu Leu Thr Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Pro Lys Tyr Asn Tyr Arg Glu Arg Lys Ser Ala Thr Glu 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Lys Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys His 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Leu Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Leu Gly Ile Thr Ile Met Glu Arg Ser Arg Phe Glu 
            1140                1145                1150        
Lys Asn Pro Val Ala Phe Leu Glu Arg Lys Gly Tyr Gln Asn Ile Gln 
        1155                1160                1165            
Glu Asn Lys Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Lys Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Asn Lys Val Asn Glu Pro Glu His Ile Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Lys Asp Leu Leu His Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Asn Glu 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Glu Asp Ala Ser Val Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Val Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 114
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #111

<400> 114
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Ile Ala Asp Glu Val Lys Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Asp Asp Pro Leu Asp Val 
                165                 170                 175     
Arg Asn Thr Asp Ser Gln Lys Leu Phe Gln Glu Phe Leu Glu Ala Tyr 
            180                 185                 190         
Asp Ser Thr Gln Glu Asp Ser His Leu Ser Glu Gln Lys Ile Asp Ile 
        195                 200                 205             
Ser Asp Ile Ile Thr Glu Lys Ile Ser Lys Ser Arg Lys Val Glu Ala 
    210                 215                 220                 
Leu Leu Ala Gln Phe Pro Asn Gln Lys Lys Asn Ser Phe Phe Gly Asn 
225                 230                 235                 240 
Leu Val Ser Leu Val Phe Gly Leu Met Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Val Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asn Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asp Glu His Asn Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Asp Phe Ile Lys Ala Asn Leu Pro Asp Val Phe Lys Glu Val Phe Ser 
            340                 345                 350         
Asp Ala Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Gly Lys Val Asp 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Ile Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His Leu 
                405                 410                 415     
Ala Glu Met Lys Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Gln Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Ala Ser Phe Asn Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Ile Asp Lys Ser Ser Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Asn Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Thr Gly Glu Ala Arg Phe Phe Asp Ala Asn Leu Lys 
    530                 535                 540                 
Gln Glu Ile Phe Asp Gly Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Leu Asp Phe Leu Glu Lys Asn Phe Asp Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Ile Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Tyr Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Glu Phe Met Asp Asp 
        595                 600                 605             
Ser Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Lys Tyr Ala Asp 
625                 630                 635                 640 
Leu Phe Asp Lys Lys Val Ile Asp Lys Leu Ser Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Arg Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Asp Glu Ile Ala Lys Ala Gln Val Ile Gly Asp Ser Asp Asp Leu His 
705                 710                 715                 720 
Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         
Asn Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ala Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ala Ile Lys Asn Leu Gly Ser Asp Ile Leu Lys Glu His Pro Val Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Arg Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Asn Asp Val Val Lys Arg Met Lys Ser Phe 
865                 870                 875                 880 
Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Ala Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Arg Glu Phe Asp Glu Asn 
    930                 935                 940                 
Asp Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Asn Phe Arg Lys Glu Phe Glu Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Lys Ala Leu Leu Thr Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Pro Lys Tyr Asn Tyr Arg Glu Arg Lys Ser Ala Thr Glu 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Met Phe Lys Thr Lys Val 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Ala Trp Asp Lys Thr Lys His 
                1045                1050                1055    
Phe Ala Thr Val Lys Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Ala Arg Lys Asn Tyr Leu Asp 
    1090                1095                1100                
Thr Gln Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Val Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Leu Gly Ile Thr Ile Met Glu Arg Ser Arg Phe Glu 
            1140                1145                1150        
Asn Asn Ser Val Ala Phe Leu Glu Arg Lys Gly Tyr Gln Asn Ile Gln 
        1155                1160                1165            
Glu Asn Lys Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Lys Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Ile Asn Ser Val Asn Glu Pro Glu His Ile Asn Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Lys Asp Leu Leu His Tyr Ile Arg Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Asn Glu 
    1250                1255                1260                
Leu Phe Glu Glu Asn Glu Glu Asp Ser Val Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Val Leu Ser Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 115
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #112

<400> 115
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Met 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         
Gly Glu Arg His Pro Ile Phe Gly Asn Ile Ala Asp Glu Val Lys Tyr 
        115                 120                 125             
His Glu Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Asp Glu Pro Ile Asp Ile 
                165                 170                 175     
Arg Asn Thr Asp Ser Gln Lys Leu Phe Gln Glu Phe Leu Glu Ala Tyr 
            180                 185                 190         
Asp Ser Ile Gln Glu Asp Ser His Leu Ser Glu Gln Lys Thr Asp Ile 
        195                 200                 205             
Ser Asp Ile Ile Thr Ala Lys Ile Ser Lys Ser Arg Lys Val Glu Ala 
    210                 215                 220                 
Leu Leu Ala Gln Phe Pro Asn Gln Lys Lys Asn Ser Phe Phe Gly Asn 
225                 230                 235                 240 
Leu Val Ser Leu Val Phe Gly Leu Met Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Met Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Val Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Thr Asn Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Asp Glu His Asn Lys Asp Lys Gln Leu Leu Lys 
                325                 330                 335     
Asp Phe Ile Arg Ala Asn Ile Gly Asp Val Phe Lys Glu Val Phe Tyr 
            340                 345                 350         
Asp Ala Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Gly Lys Val Asp 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Ile Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His Leu 
                405                 410                 415     
Ala Glu Met Lys Ala Ile Leu Arg Arg Gln Gly Glu Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Gln Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Ser Phe Asn Ser Asn Glu Lys Ile Thr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Val Ile Asp Lys Ser Ser Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Asn Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Thr Gly Glu Ala Arg Phe Phe Asp Ala Asn Leu Lys 
    530                 535                 540                 
Lys Glu Ile Phe Asp Gly Leu Phe Lys Lys Asn Arg Lys Val Thr Lys 
545                 550                 555                 560 
Lys Lys Leu Leu Asp Phe Leu Glu Lys Asn Phe Asp Glu Phe Arg Ile 
                565                 570                 575     
Val Asp Ile Thr Gly Leu Asp Asn Glu Phe Asn Ala Ser Tyr Gly Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Glu Phe Met Asp Asp 
        595                 600                 605             
Ser Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Leu Lys Tyr Glu Asp 
625                 630                 635                 640 
Leu Phe Asp Lys Lys Val Ile Asp Lys Leu Ser Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Lys Asp Lys 
            660                 665                 670         
Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly His Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe Lys 
    690                 695                 700                 
Asp Glu Ile Ala Lys Ala Gln Val Ile Gly Asp Gly Asp Asp Leu His 
705                 710                 715                 720 
Gln Val Val Gln Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Tyr 
            740                 745                 750         
Asn Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ala Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Gly Arg Leu Lys Glu 
    770                 775                 780                 
Ala Ile Gln Asp Leu Gly Ser Asp Ile Leu Lys Glu His Pro Val Glu 
785                 790                 795                 800 
Asn Asn Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Arg Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asp Asn Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Val Ser Ser Ala Lys Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Asn Asp Val Val Lys Arg Met Lys Ser Phe 
865                 870                 875                 880 
Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Ala Asp Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Arg Glu Phe Asp Glu Asn 
    930                 935                 940                 
Asp Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Asn Phe Arg Lys Glu Phe Glu Phe Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Lys Ala Leu Leu Thr Arg Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Glu Tyr Pro Lys Tyr Asn Tyr Arg Glu Arg Lys Ser Ala Thr Glu 
    1010                1015                1020                
Lys Met Phe Phe Tyr Ser Asn Ile Met Asn Met Phe Lys Thr Thr Ile 
1025                1030                1035                1040
Lys Leu Ala Asp Glu Thr Gly Glu Ile Ala Trp Asp Lys Thr Lys His 
                1045                1050                1055    
Phe Ala Thr Val Lys Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Ala Arg Lys Asn Tyr Leu Asn 
    1090                1095                1100                
Thr Gln Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Phe Val Ala Asp Ile Glu Lys Gly Lys Ser Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Met Ile Gly Ile Thr Ile Met Glu Arg Ser Arg Phe Glu 
            1140                1145                1150        
Ser Asn Ser Val Ala Phe Leu Glu Glu Lys Gly Tyr Gln Asn Ile Arg 
        1155                1160                1165            
Glu Asn Thr Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Lys Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys His Ile Asn Ser Val Asn Lys Pro Glu His Ile Asn Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Thr Asp Leu Leu His His Ile Arg Asp Phe 
        1235                1240                1245            
Ser Glu Lys Asn Ile Leu Ala Ala Lys Asn Leu Glu Lys Ile Asn Glu 
    1250                1255                1260                
Ala Phe Glu Glu Asn Glu Glu Asp Ser Val Asn Glu Leu Ala Gln Ser 
1265                1270                1275                1280
Phe Ile Ser Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Phe Asp Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Val Leu Ser Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Glu 
    1330                1335                1340

<210> 116
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #113

<400> 116
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Arg Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys His Pro Ile Phe Gly Thr Leu Ala Glu Glu Lys Ala Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Asn Asp Val Gln Lys Leu Phe Gln Glu Phe Val Glu Ile Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Gly Ser His Leu Ser Glu Gln Asn Ala Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Ile Leu Lys Leu Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Phe Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Gln Asn Leu Pro Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Met Arg Asp Tyr Phe Leu Asp Ser Gly Gln Lys 
    530                 535                 540                 
Lys Glu Ile Val Asn His Leu Phe Lys Glu Lys Arg Lys Val Thr Glu 
545                 550                 555                 560 
Lys Asp Ile Ile Asp Tyr Leu Glu Asn Glu Ile Asp Glu Tyr Ala Gly 
                565                 570                 575     
Val Glu Leu Lys Gly Leu Glu Lys Gln Phe Asn Ala Ser Leu Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Glu Phe Leu Asp Asp 
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser Asp 
625                 630                 635                 640 
Ile Phe Thr Glu Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Thr Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Thr Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu Lys 
705                 710                 715                 720 
Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu Asp 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro Thr Asp 
785                 790                 795                 800 
Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Gln Leu Ser 
            820                 825                 830         
Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Arg Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Glu Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Ala Arg Phe Asn Thr Glu Leu Asp Glu Asn 
    930                 935                 940                 
Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Pro Lys Tyr Asn Tyr Arg Glu Arg Lys Lys Ala Thr Glu 
    1010                1015                1020                
Lys Val Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Lys Glu Val 
1025                1030                1035                1040
Lys Tyr Ala Asp Glu Thr Gly Glu Ile Val Trp Asp Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Leu Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Ile Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Ile Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ala Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Ala Phe Leu Glu Arg Lys Gly Tyr Lys Asn Ile Gln 
        1155                1160                1165            
Glu Asn Asn Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Met Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile His Lys Ile Asn Glu Pro Ile His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Lys Glu Leu Leu Asn Tyr Ile Ser Asp Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Leu Gly Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr 
            1300                1305                1310        
Glu Ile Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 117
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #114

<400> 117
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Arg Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Thr Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys Tyr Pro Ile Phe Gly Thr Leu Ala Glu Glu Lys Ala Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Phe Asn Ser 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Ile Phe Gln Glu Phe Val Glu Ile Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Gly Ser His Leu Ser Glu Gln Asn Ala Gln Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 
Ile Leu Lys Leu Phe Pro Asp Glu Lys Asn Asn Gly Leu Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     
Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Met Arg Asp Tyr Phe Leu Asp Ser Gly Gln Lys 
    530                 535                 540                 
Lys Glu Ile Val Asn His Leu Phe Lys Glu Lys Arg Lys Val Thr Glu 
545                 550                 555                 560 
Lys Asp Ile Ile Asp Tyr Leu His Asn Glu Ile Asp Gly Tyr Asp Gly 
                565                 570                 575     
Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Leu Asp Asp 
        595                 600                 605             
Ala Lys Asn Glu Ala Ile Leu Glu Asp Ile Val His Thr Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Lys Tyr Asp Asn 
625                 630                 635                 640 
Ile Phe Asp Glu Lys Val Leu Lys Lys Leu Thr Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Thr Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Thr Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Val Lys 
705                 710                 715                 720 
Gln Val Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Asn Gln Gly Lys Arg Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu Asp 
    770                 775                 780                 
Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu Asn Pro Thr Asp 
785                 790                 795                 800 
Asn Asn Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Gln Leu Ser 
            820                 825                 830         
Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Asp Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Arg Lys Ser Phe 
865                 870                 875                 880 
Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Glu Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Ile Leu Asp Ala Arg Phe Asn Thr Glu Leu Asp Glu Asn 
    930                 935                 940                 
Asp Lys Arg Ile Arg Thr Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Pro Lys Tyr Asn Tyr Arg Glu Arg Lys Lys Ala Thr Glu 
    1010                1015                1020                
Lys Val Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Lys Glu Val 
1025                1030                1035                1040
Lys Tyr Ala Asp Glu Thr Gly Glu Ile Val Trp Asn Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Ile Tyr Leu Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Ile Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Ile Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Arg Ala Ala Phe Glu 
            1140                1145                1150        
Lys Asn Pro Ile Thr Phe Leu Glu Glu Lys Gly Tyr Lys Asn Ile Gln 
        1155                1160                1165            
Glu Asn Asn Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Ile Val Leu Pro Ala His Leu Val Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile His Lys Ile Asn Glu Pro Ile His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Lys Glu Leu Leu Asn Tyr Ile Ser Glu Phe 
        1235                1240                1245            
Ser Glu Lys Tyr Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ser 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Glu Asp Ala Asp Ile Asp Glu Leu Ala Ser Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Leu Gly Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Ser 
            1300                1305                1310        
Glu Ile Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 118
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #115

<400> 118
Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Ile Thr Ala Glu Gly Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala Thr Leu Asp Asp Ala 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Pro Asp Asp Lys Arg 
            100                 105                 110         
Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val Glu Glu Lys Ala Tyr 
        115                 120                 125             
His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 
Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Phe Asn Ser 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp Phe Leu Asp Thr Tyr 
            180                 185                 190         
Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu Asn Ser Lys Gln Leu 
        195                 200                 205             
Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp Arg 
    210                 215                 220                 
Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser Gly Ile Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys Tyr Phe 
                245                 250                 255     
Asn Leu Asp Glu Lys Ala Pro Leu His Phe Ser Lys Glu Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly Asp Asp Tyr Ser Asp 
        275                 280                 285             
Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Gly Thr Glu Ala Pro Leu Ser Ser Ala 
305                 310                 315                 320 
Met Ile Met Arg Tyr Lys Glu His Glu Glu Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Ala Tyr Ile Arg Asn Ile Ser Leu Glu Thr Tyr Asn Glu Val Phe Lys 
            340                 345                 350         
Asp Asn Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Val Tyr Leu Lys Asn Leu Leu Ser Asn Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala Lys Phe Tyr Pro Phe 
            420                 425                 430         
Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro Trp Asn Phe Glu Asp 
465                 470                 475                 480 
Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Phe Ile Ala Glu Ser Met Arg Asp Tyr Phe Leu Asp Ser Lys Gln Lys 
    530                 535                 540                 
Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp Lys Arg Lys Val Thr Asp 
545                 550                 555                 560 
Lys Asp Ile Ile Glu Tyr Leu His Ala Glu Ile Asp Gly Tyr Asp Gly 
                565                 570                 575     
Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ser Ser Leu Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys Glu Phe Leu Asp Asp 
        595                 600                 605             
Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile His Thr Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ser Lys Phe Glu Asn 
625                 630                 635                 640 
Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Glu 
            660                 665                 670         
Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ile Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Lys Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp Lys Asp Asn Ile Lys 
705                 710                 715                 720 
Gln Val Val Gln Ala Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly Arg 
            740                 745                 750         
Lys Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln Tyr Thr 
        755                 760                 765             
Asn Gln Gly Lys Ser Asn Ser Gln Gln Arg Leu Lys Arg Leu Glu Glu 
    770                 775                 780                 
Ser Leu Lys Glu Leu Gly Ser Lys Ile Leu Lys Glu Asn Ile Ile Asp 
785                 790                 795                 800 
Asn Asn Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Arg Leu Ser 
            820                 825                 830         
Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Leu Lys Asp Asn 
        835                 840                 845             
Ser Ile Asp Asn Lys Val Leu Val Ser Ser Ala Ser Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asp Val Pro Ser Leu Glu Val Val Lys Lys Arg Lys Thr Leu 
865                 870                 875                 880 
Trp Tyr Gln Leu Leu Lys Ser Lys Leu Ile Ser Gln Arg Lys Phe Asp 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Arg Glu Asp Lys Ala 
            900                 905                 910         
Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Arg Leu Leu Asp Glu Arg Phe Asn Asn Lys Lys Asp Glu Asn 
    930                 935                 940                 
Asn Lys Thr Val Arg Thr Val Lys Ile Ile Thr Leu Lys Ser Ser Leu 
945                 950                 955                 960 
Val Ser Gln Phe Arg Lys Asp Phe Glu Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Phe His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Ser Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Pro Lys Tyr Asn Phe Arg Glu Arg Lys Ser Ala Thr Glu 
    1010                1015                1020                
Lys Val Tyr Phe Tyr Ser Asn Ile Met Asn Ile Phe Lys Lys Ser Ile 
1025                1030                1035                1040
Ser Leu Ala Asp Glu Thr Gly Glu Ser Val Trp Asn Lys Glu Ser Asp 
                1045                1050                1055    
Leu Ala Thr Val Arg Lys Val Leu Ser Tyr Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Val Glu Glu Gln Asn His Gly Leu Asp Arg Lys Gly Leu Phe 
        1075                1080                1085            
Asn Ala Asn Leu Ser Ser Lys Pro Lys Pro Asn Ser Glu Tyr Leu Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Tyr Ala Gly Ile Ser Asn Ser Phe Ala Val 
1105                1110                1115                1120
Leu Val Lys Gly Thr Ile Glu Lys Gly Ala Lys Lys Lys Ile Thr Asn 
                1125                1130                1135    
Val Leu Glu Phe Gln Gly Ile Ser Ile Leu Asp Arg Ile Thr Tyr Arg 
            1140                1145                1150        
Lys Asp Lys Leu Asn Phe Leu Leu Glu Lys Gly Tyr Lys Asp Ile Glu 
        1155                1160                1165            
Glu Asn Leu Ile Ile Glu Leu Pro Lys Tyr Ser Leu Phe Glu Leu Ser 
    1170                1175                1180                
Asp Gly Ser Arg Arg Met Leu Ala Ser Ile Gly Glu Ile His Lys Gly 
1185                1190                1195                1200
Asn Gln Ile Phe Leu Ser Gln Lys Phe Val Lys Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile Ser Asn Thr Ile Asn Glu Asn His Arg Lys Tyr Val Glu 
            1220                1225                1230        
Asn His Lys Lys Glu Phe Glu Glu Leu Phe Tyr Tyr Ile Leu Glu Phe 
        1235                1240                1245            
Asn Glu Asn Tyr Val Gly Ala Lys Lys Asn Gly Glu Leu Leu Lys Ser 
    1250                1255                1260                
Ala Phe Gln Ser Trp Gln Asn His Ser Ile Asp Glu Leu Cys Ser Ser 
1265                1270                1275                1280
Phe Ile Gly Leu Phe Glu Leu Thr Ser Arg Gly Ser Ala Ala Asp Phe 
                1285                1290                1295    
Glu Phe Leu Gly Val Lys Ile Pro Arg Tyr Arg Tyr Thr Pro Ser Ser 
            1300                1305                1310        
Leu Leu Lys Asp Ala Thr Leu Ile His Gln Ser Val Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ala Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 119
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #116

<400> 119
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Arg Phe Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Ser Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ala Phe Asp Ile 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln Val 
        195                 200                 205             
Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     
Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Asp Glu Val Phe Ser 
            340                 345                 350         
Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Asn Ser Asp Glu Ala Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Ile Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Leu Arg Asp Tyr Phe Leu Asp Ser Gly Gln Lys 
    530                 535                 540                 
Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val Thr Glu 
545                 550                 555                 560 
Lys Asp Ile Ile His Tyr Leu His Asn Glu Val Asp Gly Tyr Asp Gly 
                565                 570                 575     
Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp Asp 
        595                 600                 605             
Ala Lys Asn Glu Ala Ile Leu Glu Asn Ile Val His Thr Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp Ser 
625                 630                 635                 640 
Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Thr Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Lys Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe Lys 
    690                 695                 700                 
Glu Ile Ile Gln Lys Ala Gln Val Ile Gly Lys Thr Asp Asp Val Lys 
705                 710                 715                 720 
Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu Asp 
    770                 775                 780                 
Ala Leu Lys Asn Leu Ala Ser Gly Ile Leu Lys Glu Asn Pro Thr Asp 
785                 790                 795                 800 
Asn Asn Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Glu Ala Leu Asp Ile Asn Gln Leu Ser 
            820                 825                 830         
Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys Asp Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Gln Lys Arg Lys Ala Phe 
865                 870                 875                 880 
Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Glu Arg Lys Phe Asn 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp Glu Arg Asp Lys Val 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Val Asn Glu Lys 
    930                 935                 940                 
Asp Lys Lys Asn Arg Thr Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Asn Phe Arg Lys Glu Phe Lys Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Lys Ala Ile Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Gln Lys Tyr Asp Pro Lys Glu Val Glu Lys Ala Thr Glu 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Leu Asn Phe Phe Lys Glu Glu Val 
1025                1030                1035                1040
His Tyr Ala Asp Asp Thr Gly Glu Ile Ala Trp Asn Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Ile Lys Lys Val Leu Ser Leu Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu 
        1075                1080                1085            
Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Ile Ser Leu Asp 
    1090                1095                1100                
Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Ile Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Ile Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Thr 
                1125                1130                1135    
Val Lys Glu Leu Val Gly Ile Thr Ile Met Glu Lys Ala Ala Phe Glu 
            1140                1145                1150        
Glu Asn Pro Ile Thr Phe Leu Glu Glu Lys Gly Tyr Lys Asn Ile Arg 
        1155                1160                1165            
Lys Asn Asn Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu 
    1170                1175                1180                
Asn Gly Arg Arg Arg Leu Leu Ala Ser Ala Lys Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Ile Val Leu Pro Val His Leu Thr Thr Leu Leu Tyr His Ala 
                1205                1210                1215    
Lys Arg Ile His Lys Ile Asn Glu Pro Ile His Leu Glu Tyr Val Glu 
            1220                1225                1230        
Lys His Arg Asn Glu Phe Lys Glu Leu Leu Asn Leu Ile Ser Glu Phe 
        1235                1240                1245            
Ser Gln Lys Tyr Ile Leu Ala Asp Asn Asn Leu Glu Lys Ile Lys Ser 
    1250                1255                1260                
Leu Tyr Glu Glu Asn Glu Asp Ala Asp Ile Asp Gln Leu Ala Asn Ser 
1265                1270                1275                1280
Phe Ile Asn Leu Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe 
                1285                1290                1295    
Lys Phe Leu Gly Lys Asp Ile Asp Arg Lys Arg Tyr Thr Ser Val Ser 
            1300                1305                1310        
Glu Ile Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 120
<211> 1340
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1340 residue - joint ancestral sequence reconstruction method–
      node #117

<400> 120
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Ser Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu Tyr 
        115                 120                 125             
His Lys Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp Ile 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln Val 
        195                 200                 205             
Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 
Val Leu Lys Leu Phe Ser Asp Glu Lys Ser Thr Gly Leu Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     
Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala Asp 
        275                 280                 285             
Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Asp Glu Val Phe Ser 
            340                 345                 350         
Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Ile Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Leu Arg Asp Tyr Phe Leu Asp Ser Gly Gln Lys 
    530                 535                 540                 
Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val Thr Glu 
545                 550                 555                 560 
Lys Asp Ile Ile His Tyr Leu His Asn Glu Val Asp Gly Tyr Asp Gly 
                565                 570                 575     
Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp Asp 
        595                 600                 605             
Ala Lys Asn Glu Ala Ile Leu Glu Asn Ile Ile His Thr Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp Ser 
625                 630                 635                 640 
Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         
Gln Thr Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Asp Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe Lys 
    690                 695                 700                 
Glu Ile Ile Gln Lys Ala Gln Val Val Gly Lys Thr Asp Asp Val Lys 
705                 710                 715                 720 
Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     
Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly His 
            740                 745                 750         
Asn Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr 
        755                 760                 765             
Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile Glu Asp 
    770                 775                 780                 
Ala Leu Lys Asn Leu Ala Pro Gly Ile Leu Lys Glu Asn Pro Thr Asp 
785                 790                 795                 800 
Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln Asn 
                805                 810                 815     
Gly Lys Asp Met Tyr Thr Gly Lys Ala Leu Asp Ile Asn Gln Leu Ser 
            820                 825                 830         
Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asp 
        835                 840                 845             
Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys Asp Asn Arg Gly Lys 
    850                 855                 860                 
Ser Asp Asn Val Pro Ser Ile Glu Val Val Gln Lys Arg Lys Ala Phe 
865                 870                 875                 880 
Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Glu Arg Lys Phe Asn 
                885                 890                 895     
Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp Glu Arg Asp Lys Val 
            900                 905                 910         
Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His 
        915                 920                 925             
Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Val Asn Glu Lys 
    930                 935                 940                 
Asp Lys Lys Asn Arg Thr Val Lys Ile Ile Thr Leu Lys Ser Asn Leu 
945                 950                 955                 960 
Val Ser Asn Phe Arg Lys Glu Phe Lys Leu Tyr Lys Val Arg Glu Ile 
                965                 970                 975     
Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Ala 
            980                 985                 990         
Lys Ala Ile Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe Val Tyr 
        995                 1000                1005            
Gly Asp Tyr Gln Lys Tyr Asp Pro Lys Asp Val Glu Lys Ala Thr Glu 
    1010                1015                1020                
Lys Tyr Phe Phe Tyr Ser Asn Leu Leu Asn Phe Phe Lys Glu Glu Val 
1025                1030                1035                1040
His Tyr Ala Asp Asp Thr Gly Glu Ile Ala Trp Asn Lys Glu Lys Asp 
                1045                1050                1055    
Phe Ala Thr Ile Lys Lys Val Leu Ser Leu Pro Gln Val Asn Ile Val 
            1060                1065                1070        
Lys Lys Thr Glu Glu Gln Thr Gly Gly Leu Phe Asp Asn Asn Ile Val 
        1075                1080                1085            
Ser Lys Lys Asp Ala Ser Lys Leu Ile Pro Ile Lys Ser Ser Leu Ser 
    1090                1095                1100                
Pro Glu Lys Tyr Gly Gly Tyr Ala Arg Pro Thr Ile Ala Tyr Ser Val 
1105                1110                1115                1120
Leu Val Ile Ala Asp Ile Glu Lys Gly Lys Ala Lys Lys Leu Lys Arg 
                1125                1130                1135    
Ile Lys Glu Met Val Gly Ile Thr Ile Gln Asp Lys Lys Lys Phe Glu 
            1140                1145                1150        
Ala Asn Pro Ile Thr Tyr Leu Glu Glu Arg Gly Tyr Lys Asn Ile Asn 
        1155                1160                1165            
Ser Asn Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Phe Asn 
    1170                1175                1180                
Asp Gly Gln Arg Arg Leu Leu Ala Ser Ser Ile Glu Leu Gln Lys Gly 
1185                1190                1195                1200
Asn Glu Leu Ile Leu Pro Tyr His Leu Thr Ala Leu Leu Tyr His Ala 
                1205                1210                1215    
Gln Arg Ile Asn Lys Ile Asn Glu Pro Ile His Lys Gln Tyr Val Glu 
            1220                1225                1230        
Thr His Gln Asn Glu Phe Lys Glu Leu Leu Thr Thr Ile Ile Ser Leu 
        1235                1240                1245            
Ser Lys Lys Tyr Ile Gln Lys Pro Asn Val Glu Ser Leu Leu Gln Gln 
    1250                1255                1260                
Ala Phe Glu Gln Asn Ala Asp Lys Asp Ile Tyr Gln Leu Ser Glu Ser 
1265                1270                1275                1280
Phe Ile Ser Leu Leu Lys Leu Thr Ser Phe Gly Ala Pro Gly Ala Phe 
                1285                1290                1295    
Lys Phe Leu Gly Val Glu Ile Ser Gln Ser Arg Tyr Lys Ser Val Ser 
            1300                1305                1310        
Gln Phe Leu Asn Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr 
        1315                1320                1325            
Glu Thr Arg Ile Asp Leu Ser Lys Leu Gly Glu Asp 
    1330                1335                1340

<210> 121
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #60

<400> 121
Met Lys Lys Asp Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Asp Asn Tyr Asn Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Ser Ile Gly Lys Ala Leu Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Ala Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
His Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Trp Arg Ile Asp 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Gln Glu Glu Ile Ser Lys Val Asp Pro Ser 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Asp Asp Arg His Pro Leu Phe Gly Asp Asn Glu Thr Glu Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Met Glu 
    130                 135                 140                 
Ser Asp Glu Lys His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Leu Val Gln Val Phe 
            180                 185                 190         
Asn Asp Thr Phe Glu Glu Glu His Leu Ser Glu Glu Ala Ile Asp Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Arg Ser Thr Arg Ala Lys Glu 
    210                 215                 220                 
Val Val Lys Leu Phe Gly Ser Glu Lys Lys Glu Ala Cys Ile Ser Ala 
225                 230                 235                 240 
Leu Ile Lys Leu Ile Val Gly Leu Lys Gly Asn Leu Lys Lys Val Phe 
                245                 250                 255     
Gly Asp Ala Glu Asp Thr Ala Ile His Phe Ser Lys Asp Asn Tyr Glu 
            260                 265                 270         
Glu Asp Val Glu Ala Ile Arg Asp Ile Ile Pro Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Glu Ala Ile Lys Ala Leu Tyr Asp Ala Ile Val Leu Ser Gly 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Ser Asn Thr Lys Ala Lys Val Ser Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Gln His Gln Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Asn Thr Lys Asn Gly Tyr Ser Ala Tyr Ile Glu Gly Gly Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Leu Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Asn Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Thr Ser Asp Asn Gly Val Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Ser Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Gly Arg Phe Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Lys Asp Gly Val Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Asn Cys Thr Tyr Leu Pro Gly Glu Asn Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Leu Asn Glu Leu Asn Asn Val Arg 
        515                 520                 525             
Leu Thr Ile Asp Lys Gly Lys Arg Arg Gln Arg Phe Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Arg Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Val Glu Asp Tyr Leu Lys Arg Glu Tyr Glu Glu Phe Asp 
                565                 570                 575     
Ser Ala Asp Ile Ser Gly Ile Asp Gly Glu Phe Lys Ser Ser Leu Gly 
            580                 585                 590         
Ser Tyr His Asp Phe Cys Lys Ile Val Val Lys Ala Asn Ser Leu Asp 
        595                 600                 605             
Glu Glu Asp Tyr Lys Asp Ile Ile Glu Glu Ile Ile Lys Trp Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Leu Arg Arg Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Glu Ile Leu Thr Glu Glu Gln Ile Lys Lys Ile Cys Lys Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Ile Arg Asp 
            660                 665                 670         
Lys Glu Thr Gly Lys Ser Ile Ile Asp Val Leu Arg Glu Thr Asp Ser 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Leu Ser Asp Glu Asp Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Gln Ala Asn Ala Glu Ala Glu Gly Glu Asn Leu 
705                 710                 715                 720 
His Glu Ile Val Glu Asp Leu Pro Gly Ser Pro Ala Ile Lys Arg Gly 
                725                 730                 735     
Ile Asn Gln Ala Leu Lys Ile Val Asp Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Gly His Glu Pro Lys Asn Ile Phe Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Lys Ser Ala Arg Gly Arg Arg Ser Thr Lys Lys Arg Leu Lys Arg Leu 
    770                 775                 780                 
Gln Glu Ala Leu Lys Asn Leu Gly Ser Asp Leu Leu Lys Glu Leu Leu 
785                 790                 795                 800 
Arg Asp Asp Asn Glu Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Met Gly Lys Cys Met Tyr Thr Gly Glu Pro Ile Asp Leu Asp Asp 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Val Ser Arg Glu Glu Asn Ala 
    850                 855                 860                 
Arg Lys Thr Asp Asn Phe Pro Ser Pro Glu Ile Arg Arg Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Met Leu Leu Lys Ala Gly Leu Ile Ser Lys Lys Lys 
                885                 890                 895     
Phe Asp Arg Leu Thr Arg Ala Asp Arg Gly Asp Phe Thr Asp Asp Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Thr Leu Leu Lys Gln Arg Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Arg Asn Ala Lys Ile Val Ser Val Lys Ala 
945                 950                 955                 960 
Asn Leu Val Ser Glu Phe Arg Gln Asp Phe Gly Leu Tyr Lys Cys Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Asn Ala Leu Leu Lys Arg Tyr Pro Gln Leu Ala Ala Glu Phe 
        995                 1000                1005            
Ile Lys Gly Asp Tyr Arg Lys Asn Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Met His Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Ser Phe Thr Ser Asp Val Lys Ile Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Ile Ala Thr Val Arg Lys Val Met Asn 
        1075                1080                1085            
Tyr His Gln Val Leu Ile Thr Arg Lys Val Glu Glu Glu Thr Gly Gly 
    1090                1095                1100                
Phe Phe Asp Gln Thr Ile Leu Ser Lys Gly Asn Ser Lys Lys Leu Ile 
1105                1110                1115                1120
Pro Leu Lys Lys Asn Leu Asp Pro Glu Lys Tyr Gly Gly Tyr Asn Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Glu Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Thr Val Lys Gln Leu Val Gly Ile Pro Ile 
        1155                1160                1165            
Arg Glu Arg Ala Lys Leu Glu Lys Asn Pro Ile Lys Tyr Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Val Asp Leu Leu Ile Lys Ile Pro Lys 
1185                1190                1195                1200
Asn Ser Leu Phe Glu Leu Asp Gly Gly Arg Arg Arg Ile Leu Ala Ala 
                1205                1210                1215    
Ala Lys Glu Leu Lys Asn Ala Asn Gln Leu Val Leu Pro Ala Glu Glu 
            1220                1225                1230        
Tyr Thr Leu Leu Asp Lys Val Ala Lys Ile Ile Lys Lys Asn Ser Asn 
        1235                1240                1245            
Ser Glu Ser Ile Ala Lys Lys Glu Glu Tyr Val Glu Glu His Leu Ser 
    1250                1255                1260                
Glu Phe Asp Glu Leu Leu Glu Ser Leu Ile Asp Tyr Ser Pro Lys Leu 
1265                1270                1275                1280
Ala Leu Gln Asp Lys Asn Leu Glu Lys Leu Lys Glu Ala Phe Glu Gln 
                1285                1290                1295    
Leu Asn Leu Ala Asp Lys Lys Glu Val Ala Lys Glu Ile Ile Asn Leu 
            1300                1305                1310        
Leu His Cys Thr Ala Thr Thr Ala Asn Ala Ala Leu Lys Phe Leu Gly 
        1315                1320                1325            
Gly Ser Lys Asn Arg Met Arg Tyr Thr Ser Thr Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Ser Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 122
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #61

<400> 122
Met Lys Lys Asp Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Asp Asn Tyr Asn Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Ser Ile Gly Lys Ala Leu Leu 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Ala Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
His Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Trp Arg Leu Asp 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Gln Glu Glu Ile Ser Lys Val Asp Ser Ser 
                85                  90                  95      
Phe Phe Ile Arg Leu Lys Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Asp Asp Arg His Pro Leu Phe Asp Asp Asn Glu Thr Glu Lys Asp Tyr 
        115                 120                 125             
Tyr Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Met Glu 
    130                 135                 140                 
Asn Asp Glu Lys His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Ser Ser 
                165                 170                 175     
Glu Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Leu Val Gln Val Leu 
            180                 185                 190         
Asn Asp Ala Leu Glu Glu Glu His Leu Ser Glu Glu Ala Asn Asp Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Arg Ser Thr Arg Ala Lys Glu 
    210                 215                 220                 
Val Val Lys Leu Phe Gly Ser Glu Lys Lys Glu Ala Cys Ile Ser Ala 
225                 230                 235                 240 
Leu Val Lys Ala Ile Val Gly Leu Lys Gly Asp Leu Lys Lys Val Phe 
                245                 250                 255     
Gly Glu Ala Glu Asp Thr Ala Ile His Phe Ser Lys Asp Asp Tyr Glu 
            260                 265                 270         
Glu Asp Val Glu Ala Ile Arg Asp Leu Ile Pro Asp Asp Ser Ala Asp 
        275                 280                 285             
Leu Phe Glu Ala Ile Lys Ala Leu Tyr Asp Ala Ile Val Leu Gln Gly 
    290                 295                 300                 
Leu Leu Gly Asp Ser Thr Ser Asn Thr Lys Ala Lys Val Ser Ala Ser 
305                 310                 315                 320 
Met Val Ala Arg Tyr Glu Gln His Gln Lys Asp Leu Lys Leu Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu His Leu Ser Glu Lys Tyr Asn Glu Phe Phe Ser 
            340                 345                 350         
Asp Asn Thr Asn Asn Gly Tyr Ser Ala Tyr Ile Glu Gly Gly Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Leu Leu Lys Glu Leu Glu 
    370                 375                 380                 
Asp Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Asn Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Thr Ser Asp Asn Gly Val Ile Pro Tyr Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asp Ala Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Gly Arg Phe Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Lys Asp Gly Val Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Gly Asn Cys Thr Tyr Leu Pro Gly Glu Thr Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Glu Glu Phe Thr Val Leu Asn Glu Leu Asn Asn Val Arg 
        515                 520                 525             
Leu Thr Ile Asp Lys Gly Lys Arg Arg Gln Arg Phe Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Glu Ile Leu Asp Asp Leu Phe Arg Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Val Glu Asp Tyr Leu Lys Arg Glu Tyr Glu Asn Phe Asp 
                565                 570                 575     
Ser Ala Asp Ile Ser Gly Ile Asp Gly Glu Phe Lys Ser Ser Leu Gly 
            580                 585                 590         
Ser Tyr His Asp Phe Cys Lys Ile Val Val Lys Ala Asp Tyr Leu Asp 
        595                 600                 605             
Glu Glu Asp His Lys Asp Ile Ile Glu Glu Ile Ile Lys Trp Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Leu Lys Arg Arg Leu Glu Lys Tyr Gly 
625                 630                 635                 640 
Glu Ile Leu Thr Glu Glu Gln Ile Lys Lys Ile Cys Lys Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Ile Arg Asp 
            660                 665                 670         
Ala Glu Thr Gly Lys Ser Ile Ile Asp Val Leu Arg Glu Thr Asp Pro 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Leu Ser Asp Glu Asp Leu Gly Phe 
    690                 695                 700                 
Lys Glu Glu Ile Asp Gln Leu Asn Ala Glu Ala Ala Gly Glu Asn Leu 
705                 710                 715                 720 
Gln Asp Ile Val Asp Asp Leu Pro Gly Ser Pro Ala Ile Lys Arg Gly 
                725                 730                 735     
Ile Asn Gln Ala Ile Arg Ile Val Asp Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Gly Lys Glu Pro Ser Asn Ile Phe Val Glu Met Ala Arg Glu Glu Gln 
        755                 760                 765             
Gln Ser Ala Arg Gly Arg Arg Ser Thr Lys Arg Arg Leu Lys Arg Leu 
    770                 775                 780                 
Gln Glu Ala Leu Lys Asn Leu Gly Ser Asp Leu Leu Lys Glu Leu Leu 
785                 790                 795                 800 
Arg Asp Asp Asn Asp Leu Arg Asp Asp Arg Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Met Gly Lys Cys Met Tyr Thr Gly Glu Pro Ile Asp Leu Asp Asp 
            820                 825                 830         
Leu Ser Lys Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Val Ser Arg Glu Glu Asn Ala 
    850                 855                 860                 
Arg Lys Thr Asp Asn Leu Pro Ser Pro Glu Ile Arg Arg Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Met Leu Leu Lys Ala Arg Leu Ile Ser Lys Lys Lys 
                885                 890                 895     
Phe Asp Arg Leu Thr Arg Ala Asp Arg Gly Asp Phe Thr Asp Asp Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Thr Leu Leu Glu Gln Arg Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Arg Asn Ala Lys Ile Val Ser Val Lys Ala 
945                 950                 955                 960 
Asn Leu Val Ser Glu Phe Arg Gln Asp Phe Gly Leu Tyr Lys Cys Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Asn Val Ile Leu Lys Arg Tyr Pro Ser Leu Ala Ala Asp Tyr 
        995                 1000                1005            
Ile Arg Gly Asp Tyr Arg Lys Asn Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Ala His Phe Tyr Ser 
1025                1030                1035                1040
Ser Ile Met Asn Ser Phe Thr Ser Asp Glu Lys Ile Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Ile Ala Thr Val Arg Lys Val Met Asn 
        1075                1080                1085            
Tyr Arg Gln Val Leu Ile Thr Arg Lys Val Glu Glu Glu Thr Gly Ala 
    1090                1095                1100                
Phe Phe Asp Gln Thr Ile Tyr Ser Lys Gly Ser Ser Lys Lys Leu Ile 
1105                1110                1115                1120
Pro Leu Lys Lys Asn Leu Asp Pro Asp Lys Tyr Gly Gly Tyr Ser Ser 
                1125                1130                1135    
Glu Lys Val Ala Tyr Ser Val Leu Val Glu Tyr Asp Ile Glu Lys Arg 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Gln Leu Val Gly Ile Pro Ile 
        1155                1160                1165            
Arg Glu Arg Ala Lys Leu Glu Lys Asn Pro Ser Lys Tyr Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Glu Asn Pro Lys Val Asp Leu Leu Ile Arg Ile Pro Lys 
1185                1190                1195                1200
Asn Gln Leu Leu Glu Leu Asp Gly Gly Arg Arg Arg Ile Leu Ala Ala 
                1205                1210                1215    
Ala Lys Glu Leu Lys Asn Ala Thr Gln Leu Val Leu Pro Ala Glu Glu 
            1220                1225                1230        
Tyr Thr Leu Leu Asp Lys Val Ala Lys Ile Ala Lys Lys Asn Ser Asn 
        1235                1240                1245            
Ser Glu Ser Ile Ala Lys Lys Glu Glu Tyr Val Glu Glu His Leu Ser 
    1250                1255                1260                
Glu Phe Asp Glu Leu Leu Glu Ser Leu Ile Asp Tyr Ser Pro Lys Leu 
1265                1270                1275                1280
Ala Leu Gln Asp Asn Asn Leu Glu Lys Leu Lys Glu Ala Phe Glu Gln 
                1285                1290                1295    
Leu Asn Leu Ala Asp Lys Lys Asp Val Ala Lys Glu Ile Ile Gly Leu 
            1300                1305                1310        
Leu His Cys Thr Ala Thr Thr Ala Asn Ala Ala Leu Lys Ser Leu Gly 
        1315                1320                1325            
Gly Ser Lys Asn Arg Met Arg Tyr Thr Ser Thr Lys Ser Leu Leu Asn 
    1330                1335                1340                
Ala Ser Leu Ile His Gln Ser Ile Thr Gly Leu Phe Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 123
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #62

<400> 123
Thr Thr Asp Asp Tyr Ser Ile Gly Leu Asp Met Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Ser Asn Gly Ser Met Ala His Lys Lys Met 
            20                  25                  30          
Pro Thr Leu Gly Asn Thr Asp Lys Lys Ser Ile Gly Lys Pro Thr Leu 
        35                  40                  45              
Gly Ser Arg Leu Phe Asp Ala Ala Glu Thr Ala Ala Glu Ala Arg Ala 
    50                  55                  60                  
His Arg Gly Gln Arg Arg Arg Tyr Val Arg Arg Arg Trp Arg Leu Asp 
65                  70                  75                  80  
Leu Leu Gln Gly Leu Phe Gln Asp Glu Ile Asn Lys Val Asp Ser Glu 
                85                  90                  95      
Phe Phe Ile Arg Leu Arg Gln Ser Arg Leu His Val Glu Asp Arg Ala 
            100                 105                 110         
Asp Tyr Arg Trp Pro Leu Phe Asn Asp Ser Asp Thr Glu Lys Asp Tyr 
        115                 120                 125             
Tyr Lys Arg Phe Pro Thr Ile Tyr His Leu Arg Lys Trp Leu Met Glu 
    130                 135                 140                 
Thr Asp Glu Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Val His Asn 
145                 150                 155                 160 
Ile Val Lys His Arg Gly Asn Phe Leu Arg Glu Lys Asn Leu Ser Ser 
                165                 170                 175     
His Asn Ala Asp Pro Ala Glu Ala Val Arg His Leu Arg Gln Ala Leu 
            180                 185                 190         
Asn Asp Trp Leu Glu Arg Gly Tyr Leu Ala Glu Asn Asp Ser His Ile 
        195                 200                 205             
Leu Asp Ile Leu Thr Asp Ser Glu Arg Arg Ser Asp Gln Ala Lys Glu 
    210                 215                 220                 
Ile Ala Lys Leu Phe Gly Thr Glu Gln Ser Asp Ala Cys Asn Ser Ala 
225                 230                 235                 240 
Leu Ala Lys Ala Ile Val Gly Leu Lys Ala Glu Leu Lys Asn Val Phe 
                245                 250                 255     
Gly Glu Ala Glu Lys Thr Ala Ile His Leu Ser Ser Asp Asp Tyr Glu 
            260                 265                 270         
Asp Asp Val Glu Ala Ile Arg Asp Ala Ile Pro Asp Asp Ser Gly Glu 
        275                 280                 285             
Leu Phe Gln Ala Leu Cys Ala Leu Tyr Ser Ala Tyr Val Leu Gln Gly 
    290                 295                 300                 
Leu Leu Ser Tyr Ala Pro Gly Gln Thr Lys Ala Lys Ile Ser Ala Asn 
305                 310                 315                 320 
Met Val Ala Lys Tyr Asp Gln Tyr Gly Lys Asp Leu Lys Leu Leu Lys 
                325                 330                 335     
Asn Leu Ala Arg Thr Tyr Val Ser Glu Asn Tyr Asp Ala Phe Phe Arg 
            340                 345                 350         
Gly Glu Thr Tyr Glu Gly Ser Ser Asp Tyr Val Gln Gly Tyr Ala Ser 
        355                 360                 365             
Tyr Asp Asp Phe Gln Lys Ser Val Lys Lys Leu Phe Lys Gln Leu Glu 
    370                 375                 380                 
Asp Asp Arg Tyr Met Met Lys Arg Phe Gly Glu Glu Lys Phe Leu Arg 
385                 390                 395                 400 
Arg Leu Lys Thr Ser Asp Asn Gly Ser Ile Tyr Tyr Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys His Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Glu Glu Asp Lys Ile Glu Ser Leu Val Ser Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Glu Asn Gln Arg Phe Ala Trp 
    450                 455                 460                 
Ser Ser Arg Met Lys Asp Ala Val Ile Thr Pro Trp Asn Trp Asp Glu 
465                 470                 475                 480 
Ile Ile Asp Arg Asp Lys Ser Ala Glu Glu Phe Ile Leu Arg Met Thr 
                485                 490                 495     
Gly Asp Cys Thr Tyr Leu Gln Gly Glu Pro Val Leu Pro Lys Cys Ser 
            500                 505                 510         
Leu Leu Tyr Glu Glu Phe Cys Val Leu Asn Glu Leu Asn Gly Ala Arg 
        515                 520                 525             
Trp Thr Ile Asp Glu Asp Asp Glu Arg Gln Arg Phe Asp Ala Ala Gln 
    530                 535                 540                 
Arg Glu Gly Ile Leu His Asp Leu Phe Arg Lys Thr Arg Lys Val Thr 
545                 550                 555                 560 
Tyr Lys Lys Val Ala Asp Trp Leu Glu Arg Glu Tyr Glu Asn Phe Ala 
                565                 570                 575     
Asn Ala His Val Arg Gly Gly Gln Gly Glu Phe Glu Ser Lys Leu Gly 
            580                 585                 590         
Ser Tyr Ile Phe Phe Cys Lys Asp Val Phe Lys Thr Asp Ser Leu Asp 
        595                 600                 605             
Glu Ala Asp Tyr Lys Pro Met Ile Glu Gln Ile Ile Leu Trp Asn Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Asp Ile Leu Lys Arg Lys Ile Glu Ala Tyr Gly 
625                 630                 635                 640 
Glu His Leu Thr Asp Glu Gln Ile Lys Val Ile Cys Lys Lys Arg Phe 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Ile Lys Asp 
            660                 665                 670         
Ala Asp Thr Gly Arg Ser Ile Ile Asp Val Leu Arg Glu Gly Asn Pro 
        675                 680                 685             
Asn Ser Arg Thr Phe Met Glu Val Leu Arg Asp Glu Asp Leu Gly Phe 
    690                 695                 700                 
Gln Lys Lys Ile Asp Asp Phe Asn Lys Asp Tyr Ala Gly Glu Asn Leu 
705                 710                 715                 720 
Met Asp Ile Val Asn Asp Leu Pro Gly Ser Pro Ala Ile Arg Arg Ser 
                725                 730                 735     
Ile Asn Gln Ala Ile Arg Ile Val Asp Glu Ile Val Ser Ile Ala Gly 
            740                 745                 750         
Lys Lys Glu Pro Ser Asn Ile Phe Ile Glu Val Thr Arg Glu Glu Asp 
        755                 760                 765             
Asp Pro Arg Lys Lys Gly Arg Arg Thr Lys Arg Arg Tyr Glu Asp Ile 
    770                 775                 780                 
Lys Asn Ala Leu Glu Asn Phe Lys Thr Asp Leu Trp Lys Glu Leu Leu 
785                 790                 795                 800 
Lys Lys Asp Ser Asn Asn Leu Asp Glu Arg Leu Ser Leu Tyr Phe Met 
                805                 810                 815     
Gln Arg Gly Lys Cys Met Tyr Ser Gly Arg Pro Ile Lys Leu Glu Glu 
            820                 825                 830         
Leu His Lys Tyr Glu Val Asp His Ile Ile Pro Arg Ser Tyr Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Glu Asn Lys Val Leu Val Cys Arg Glu Glu Asn Gln 
    850                 855                 860                 
Arg Lys Thr Asp Glu Leu Leu Asp Pro Ala Ile Arg Arg Lys Met Gly 
865                 870                 875                 880 
Asp Tyr Trp Arg Met Leu His Lys Ala Lys Leu Ile Gly Asp Lys Lys 
                885                 890                 895     
Phe Asn Asn Leu Phe Arg Arg Asp Arg Gly Asp Ile Asp Asp Lys Thr 
            900                 905                 910         
Leu Lys Gly Phe Val Ala Arg Gln Leu Val Glu Thr Ser Gln Met Val 
        915                 920                 925             
Lys Leu Val Gln Thr Leu Leu Glu Ala Arg Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Lys Asn Ala Lys Ile Val Pro Val Lys Ala 
945                 950                 955                 960 
Asn Ile Ser His Asp Leu Arg Glu Ala Ala Gly Leu Val Lys Cys Arg 
                965                 970                 975     
Glu Ala Asn Asp Phe His His Ala His Asp Ala Tyr Leu Ala Cys Arg 
            980                 985                 990         
Val Gly Leu Phe Ile Gln Lys Arg His Pro Ser Val Tyr Ala Asn His 
        995                 1000                1005            
Val Met Arg Asp Tyr Ala Arg Lys Gln Leu Lys Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Tyr Asn Lys Thr His Ser Met Ala His Thr Lys Gly 
1025                1030                1035                1040
Phe Ile Val Asn Ser Phe Met Ser Ser Glu Phe Asp Lys Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Lys Pro Ile Ile Glu Ile Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Phe Trp Asp Ala Glu Ala Glu Val Glu Asp Ile Arg Arg Val Leu Asn 
        1075                1080                1085            
Tyr Arg Gln Cys Phe Ile Ser Arg Met Pro Val Glu Asp Thr Gly Ala 
    1090                1095                1100                
Phe Trp Asp Gln Thr Ile Tyr Ser Pro Arg Ser Asn Lys Lys Leu Leu 
1105                1110                1115                1120
Pro Leu Lys Arg Asp Leu Asp Pro Asp Lys Tyr Gly Ser Tyr Ser Arg 
                1125                1130                1135    
Glu Gln Phe Ala Tyr Ser Val Phe Phe Ile Tyr Asp Ile Glu Ala Arg 
            1140                1145                1150        
Asp Ala Lys Lys Ser Lys Thr Val Leu Gln Phe Ala Gln Val Pro Val 
        1155                1160                1165            
Arg Val Thr Ala Ala Ile Ala Lys Asp Pro Ser Lys Tyr Ala Ser Lys 
    1170                1175                1180                
Leu Gly Gln Glu Asn Leu Glu Phe Thr Arg Ile Ile Arg Ile Leu Lys 
1185                1190                1195                1200
Lys Gln Leu Leu Glu Leu Glu Asn Gly Asp Arg Leu Ile Ile Thr Gly 
                1205                1210                1215    
Ala Lys Glu Val Arg Asn Ala Thr Glu Phe Ala Phe Ser Leu Glu Glu 
            1220                1225                1230        
Thr Thr Leu Val Glu Arg Cys Ser Gln Ile Ala Lys Glu Gly Ser Asn 
        1235                1240                1245            
Pro Glu Asp Ile Ala Ser Asp Glu Glu Tyr Val Glu Gln Leu Ser Ser 
    1250                1255                1260                
Gln Leu Asn Glu Leu Phe Ser Ser Leu Ile Glu Arg Gly Pro Lys Leu 
1265                1270                1275                1280
Gly Val Arg Leu His Ala Gln Leu Lys Leu Asp Gln Tyr Leu Ser Gln 
                1285                1290                1295    
Phe Asp Asp Ala Ala Leu Arg Asp Lys Ala Lys Val Ile Ile Gly Leu 
            1300                1305                1310        
Ile Ser Ile Thr Asn Gly Ala Thr Asn Thr Ala Leu Ser Ser Leu Gly 
        1315                1320                1325            
Gly Ser Lys Asn Arg Met Arg Tyr Thr His Asn Lys Leu Leu Asn Asn 
    1330                1335                1340                
Ala Ser Leu Ile Asp Gln Ser Val Thr Gly Met Phe Glu Arg Arg Thr 
1345                1350                1355                1360
His Ile Gly Leu Leu Gly Glu Asp 
                1365            

<210> 124
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #63

<400> 124
Met Lys Lys Asp Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Asp Asn Tyr Asn Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Ser Ile Gly Lys Ala Leu Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Ala Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
His Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Gln Arg Ile Asp 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Gln Glu Glu Ile Ser Lys Val Asp Pro Ser 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Asp Asp Arg His Pro Leu Phe Gly Asp Asn Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Met Glu 
    130                 135                 140                 
Ser Asp Glu Lys His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Leu Val Gln Val Phe 
            180                 185                 190         
Asn Asp Thr Phe Glu Glu Glu His Leu Ser Glu Glu Ala Ile Asp Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Arg Ser Thr Arg Ala Lys Glu 
    210                 215                 220                 
Val Val Lys Leu Phe Gly Ser Glu Lys Lys Gln Ala Cys Ile Ser Ala 
225                 230                 235                 240 
Leu Ile Lys Leu Ile Val Gly Leu Lys Gly Asn Leu Lys Lys Val Phe 
                245                 250                 255     
Gly Asp Ala Glu Asp Thr Ser Ile His Phe Ser Lys Asp Asn Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Ile Arg Asp Ile Ile Pro Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Glu Ala Ile Lys Ala Leu Tyr Asp Ala Ile Val Leu Ser Gly 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Ser Asn Thr Lys Ala Lys Val Ser Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Gln His Gln Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Asn Thr Lys Asn Gly Tyr Ser Ala Tyr Ile Glu Gly Gly Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Asn Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Thr Ser Asp Asn Gly Val Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Ser Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Gly Arg Phe Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Lys Asp Gly Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Asn Cys Thr Tyr Leu Pro Gly Glu Asn Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Leu Asn Glu Leu Asn Asn Val Arg 
        515                 520                 525             
Leu Thr Thr Asp Lys Gly Lys Thr Arg Gln Arg Phe Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Arg Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Glu Asp Tyr Leu Lys Arg Glu Tyr Glu Glu Phe Asp 
                565                 570                 575     
Ser Ala Asp Ile Ser Gly Ile Asp Gly Glu Phe Lys Ser Ser Leu Gly 
            580                 585                 590         
Ser Tyr His Asp Phe Cys Lys Ile Val Val Lys Gly Asn Ser Leu Asp 
        595                 600                 605             
Glu Glu Asp Tyr Lys Asp Ile Ile Glu Glu Ile Ile Lys Trp Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Leu Arg Arg Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Glu Ile Leu Thr Glu Glu Gln Ile Lys Lys Ile Cys Lys Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Ile Arg Asp 
            660                 665                 670         
Lys Glu Thr Gly Lys Ser Ile Ile Asp Val Leu Arg Glu Thr Asp Ser 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Leu Ser Asp Glu Asp Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Gln Ala Asn Ala Glu Ala Glu Gly Glu Asn Leu 
705                 710                 715                 720 
His Glu Ile Val Glu Asp Leu Pro Gly Ser Pro Ala Ile Lys Arg Gly 
                725                 730                 735     
Ile Leu Gln Ala Leu Lys Ile Val Asp Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Gly His Glu Pro Lys Asn Ile Phe Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Lys Thr Gly Arg Gly Arg Arg Ser Thr Lys Lys Arg Leu Lys Arg Leu 
    770                 775                 780                 
Gln Glu Ala Leu Lys Asn Leu Gly Ser Asn Leu Leu Lys Glu Leu Pro 
785                 790                 795                 800 
Arg Asp Asp Asn Glu Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Met Gly Lys Cys Met Tyr Thr Gly Glu Pro Ile Asp Leu Asp Asp 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Val Ser Arg Glu Glu Asn Ala 
    850                 855                 860                 
Arg Lys Ser Asp Asn Phe Pro Ser Pro Glu Ile Arg Arg Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Met Leu Leu Lys Ala Gly Leu Ile Ser Lys Lys Lys 
                885                 890                 895     
Phe Asp Arg Leu Thr Arg Ala Asp Arg Gly Asp Phe Thr Asp Asp Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Thr Leu Leu Lys Gln Arg Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Arg Asn Ala Lys Ile Val Ser Val Lys Ala 
945                 950                 955                 960 
Asn Leu Val Ser Glu Phe Arg Gln Asp Phe Gly Leu Tyr Lys Cys Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Asn Ala Leu Leu Lys Lys Tyr Pro Gln Leu Ala Ala Glu Phe 
        995                 1000                1005            
Ile Lys Gly Asp Tyr Arg Lys Asn Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Met His Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Ser Phe Thr Ser Asp Val Lys Ile Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Ile Ala Thr Val Arg Lys Val Met Asn 
        1075                1080                1085            
Tyr His Gln Val Leu Ile Thr Arg Lys Val Glu Glu Glu Lys Gly Gly 
    1090                1095                1100                
Phe Phe Asp Gln Thr Ile Leu Ser Lys Gly Asn Ser Lys Lys Leu Ile 
1105                1110                1115                1120
Pro Leu Lys Lys Asn Leu Asp Pro Glu Lys Tyr Gly Gly Tyr Asn Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Glu Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Thr Val Lys Gln Leu Val Gly Ile Pro Ile 
        1155                1160                1165            
Arg Glu Arg Ala Lys Leu Glu Lys Asn Pro Ile Lys Tyr Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Val Asp Leu Leu Ile Lys Ile Pro Lys 
1185                1190                1195                1200
Asn Ser Leu Phe Glu Leu Asp Gly Gly Arg Arg Arg Ile Leu Ala Ala 
                1205                1210                1215    
Ala Lys Glu Leu Lys Asn Ala Asn Gln Leu Val Leu Pro Ala Glu Glu 
            1220                1225                1230        
Tyr Thr Leu Leu Asp Lys Val Ala Lys Ile Ile Lys Lys Asn Ser Asn 
        1235                1240                1245            
Ser Glu Ser Ile Ala Lys Lys Glu Glu Tyr Val Glu Glu His Leu Ser 
    1250                1255                1260                
Glu Phe Asp Glu Leu Leu Glu Ser Leu Ile Asp Tyr Ser Pro Lys Leu 
1265                1270                1275                1280
Ala Leu Gln Asp Lys Asn Leu Glu Lys Ile Lys Glu Ala Phe Glu Gln 
                1285                1290                1295    
Leu Asn Leu Ala Asp Lys Lys Glu Val Ala Lys Glu Ile Ile Asn Leu 
            1300                1305                1310        
Leu His Cys Thr Ala Thr Thr Ala Asn Ala Ala Leu Lys Phe Leu Gly 
        1315                1320                1325            
Gly Ser Lys Asn Arg Met Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Ser Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 125
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> Sequence1368 residue - marginal ancestral sequence reconstruction
      method– node #64

<400> 125
Met Lys Lys Glu Tyr Tyr Ile Gly Leu Asp Met Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Cys Lys Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Thr Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Tyr Arg Thr Ser Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ser Glu Glu Ile Ser Lys Val Asp Pro Thr 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Glu Asp Arg Tyr Pro Leu Phe Val Asp Asn Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Met Glu 
    130                 135                 140                 
Asn Asp Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asp Ser 
                165                 170                 175     
Glu Asn Thr Asp Leu Ser Glu Val Phe Lys Glu Leu Val Lys Val Phe 
            180                 185                 190         
Asn Asp Glu Leu Glu Ile Glu Leu Leu Ser Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Lys Ser Ala Lys Ala Lys Glu 
    210                 215                 220                 
Ile Ala Lys Leu Phe Gly Ser Glu Lys Lys Gln Ala Cys Ile Lys Ala 
225                 230                 235                 240 
Leu Val Lys Leu Ile Val Gly Leu Thr Gly Asn Leu Ser Glu Ile Phe 
                245                 250                 255     
Gly Asp Ser Glu Glu Thr Ser Ile Asn Phe Ser Glu Ser Asn Tyr Glu 
            260                 265                 270         
Glu Asn Met Glu Ala Ile Glu Glu Ile Leu Pro Asp Glu Phe Ser Glu 
        275                 280                 285             
Leu Ile Glu Ala Ile Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asp Leu Gln Gln Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Thr His Cys Pro Glu Glu Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Lys Asn Gly Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Glu Glu Leu Glu 
    370                 375                 380                 
Lys Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Gln Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Ser Ile Phe Lys Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Gly Asn Gly Ser Asn Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Glu Asp Gly Lys Ile Tyr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Val Val Asp Val Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Glu Lys Ile Lys Lys Arg Gln Arg Phe Ser Val Glu Leu 
    530                 535                 540                 
Lys Gln Glu Ile Tyr Asn Asp Leu Phe Cys Arg Asn Arg Lys Val Thr 
545                 550                 555                 560 
Gly Lys Lys Ile Lys Asp Tyr Leu Lys Arg Glu Tyr Asn Glu Ile Asp 
                565                 570                 575     
Glu Ala Asp Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ser Leu Arg 
            580                 585                 590         
Ser Tyr His Asp Phe Lys Lys Ile Val Gly Lys Gly Asn Ser Leu Asp 
        595                 600                 605             
Glu Glu Asp Tyr Lys Glu Ile Ile Glu Glu Ile Ile Lys Trp Ile Thr 
    610                 615                 620                 
Ile Phe Asp Asp Lys Lys Met Leu Lys Arg Arg Leu Lys Lys Tyr Pro 
625                 630                 635                 640 
Glu Ile Leu Ser Glu Glu Gln Ile Lys Lys Ile Cys Lys Leu Lys Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Thr Gly Val Arg Asp 
            660                 665                 670         
Lys Glu Thr Gly Glu Ser Ile Ile Asn Ala Leu Trp Glu Thr Asp Ser 
        675                 680                 685             
Ser Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Lys Asp Tyr Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Gln Ile Asn Ala Glu Ala Ala Gly Glu Asn Ser 
705                 710                 715                 720 
Tyr Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Ala 
                725                 730                 735     
Ile Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Gly His Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Glu Lys Glu 
        755                 760                 765             
Lys Asn Gly Lys Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu 
    770                 775                 780                 
Tyr Lys Ser Cys Lys Asn Asp Ala Ser Asp Leu Leu Lys Glu Leu Gly 
785                 790                 795                 800 
Arg Asp Asp Asn Glu Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Met Gly Lys Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Asp Asp 
            820                 825                 830         
Leu Phe Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Val Arg Lys Glu Val Asn Ala 
    850                 855                 860                 
Lys Lys Ser Asp Asn Phe Pro Ser Pro Glu Ile Arg Glu Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Met Leu Leu Lys Lys Gly Phe Ile Ser Lys Lys Lys 
                885                 890                 895     
Tyr Asp Arg Leu Thr Arg Thr Thr Arg Gly Glu Phe Thr Asp Asp Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr 
        915                 920                 925             
Lys Ala Val Ala Glu Leu Leu Lys Gln Leu Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Arg Asp Ser Glu Ile Val Tyr Val Lys Ala 
945                 950                 955                 960 
Asn Leu Val Ser Glu Phe Arg Gln Asp Phe Asp Leu Ile Lys Cys Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val 
            980                 985                 990         
Val Gly Asn Val Tyr His Val Lys Tyr Thr Asn Asn Ala Ala Lys Phe 
        995                 1000                1005            
Ile Lys Gly Asn Arg Gly Arg Asn Tyr Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Met His Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Lys Lys Met Phe Thr Ser Asp Val Lys Ser Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Lys Asn Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Asp Lys Ser Ile Ala Thr Val Arg Lys Ile Met Asn 
        1075                1080                1085            
Lys Asn Asn Ile Leu Tyr Thr Arg Tyr Thr Tyr Glu Glu Lys Gly Gly 
    1090                1095                1100                
Leu Phe Asn Gln Thr Ile Leu Lys Lys Gly Asn Ser Gln Lys Leu Ile 
1105                1110                1115                1120
Pro Leu Lys Lys Asn Leu Asp Pro Glu Lys Tyr Gly Gly Tyr Asn Ser 
                1125                1130                1135    
Pro Thr Ala Ala Tyr Phe Ala Leu Val Glu Tyr Asp Ile Glu Glu Gly 
            1140                1145                1150        
Lys Lys Arg Lys Arg Lys Thr Thr Lys Gln Leu Val Ala Ile Pro Ile 
        1155                1160                1165            
Tyr Glu Lys Ala Lys Leu Glu Glu Asn Pro Ile Lys Tyr Leu Glu Glu 
    1170                1175                1180                
Arg Gly Leu Lys Asn Pro Ala Val Asp Leu Leu Ile Lys Ile Lys Lys 
1185                1190                1195                1200
Asn Ser Leu Phe Glu Met Asp Gly Phe Arg Met Arg Leu Ser Gly Ala 
                1205                1210                1215    
Thr Ile Val Phe Lys Asn Ala Val Gln Leu Val Leu Asp Glu Glu His 
            1220                1225                1230        
Tyr Glu Ile Leu Lys Lys Val Ala Lys Val Leu Glu Lys Asn Ser Glu 
        1235                1240                1245            
Asn Gln Thr Ile Ala Lys Lys Glu Gly Tyr Thr Glu Glu Leu Leu Ser 
    1250                1255                1260                
Leu Tyr Asp Glu Leu Leu Glu Lys Leu Lys Asp Tyr Lys Asn Arg Leu 
1265                1270                1275                1280
Ala Asn Gln Ala Lys Thr Leu Glu Lys Gly Lys Glu Lys Phe Glu Lys 
                1285                1290                1295    
Leu Asn Leu Glu Asp Lys Ala Glu Val Ile Asn Glu Ile Ile Asn Leu 
            1300                1305                1310        
Phe His Cys Asn Ala Thr Thr Ala Asn Asn Ala Leu Lys Leu Ile Gly 
        1315                1320                1325            
Gly Ser Lys Asn Arg Met Lys Asn Asn Asn Ile Ser Lys Ser Asn Lys 
    1330                1335                1340                
Val Ser Leu Ile Asn Gln Ser Ile Thr Gly Leu Tyr Glu Thr Lys Ile 
1345                1350                1355                1360
Asp Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 126
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> Sequence1368 residue - marginal ancestral sequence reconstruction
      method– node #65

<400> 126
Met Lys Lys Glu Tyr Tyr Leu Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Val Lys Arg His Val 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Ile Arg Leu Phe Glu Thr Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Tyr Arg Ser Ser Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Asp 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ser Glu Glu Ile Ala Lys Val Asp Leu Thr 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Glu Glu Lys Tyr Pro Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Ile Glu 
    130                 135                 140                 
Asn Pro Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Ile Glu Gly Asp Ser Asp Asn 
                165                 170                 175     
Lys Asn Thr Asp Leu Ser Glu Val Phe Ser Glu Leu Val Lys Ala Phe 
            180                 185                 190         
Asn Asp Val Ile Glu Tyr Glu Ile Leu Ser Thr Glu Ala Leu Glu Leu 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Thr Ser Lys Ser Ala Lys Ala Lys Glu 
    210                 215                 220                 
Ile Ala Lys Leu Phe Glu Asp Glu Lys Glu Gln Gln Cys Ile Lys Ala 
225                 230                 235                 240 
Leu Ser Lys Leu Ile Val Gly Leu Thr Gly Asn Ile Ser Glu Met Phe 
                245                 250                 255     
His Gln Ser Glu Glu Thr Ser Ile Asn Phe Ser Glu Ser Asn Tyr Glu 
            260                 265                 270         
Glu Asn Met Glu Ala Ile Glu Glu Ile Ile Pro Asp Glu Phe Ser Glu 
        275                 280                 285             
Leu Ile Asp Ala Ile Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Glu 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asn Leu Lys Gln Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Thr Tyr Cys Ser Glu Glu Tyr Lys Glu Phe Phe Ser 
            340                 345                 350         
Ser Asp Glu Lys Asn Gly Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Glu Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Glu Glu Lys Glu 
    370                 375                 380                 
Lys Ala Glu Tyr Leu Leu Ala Glu Ile Glu Asn Glu Thr Leu Leu Pro 
385                 390                 395                 400 
Leu Gln Arg Ser Lys Asp Asn Gly Thr Ile Pro His Gln Val His Leu 
                405                 410                 415     
Gln Glu Leu Lys Lys Ile Leu Glu Asn Ala Ser Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Ile Ser Ile Phe Lys Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Arg Asn Gly Ser Asn Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Glu Asp Gly Arg Ile Tyr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Val Val Asp Val Glu Lys Ser Asn Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Lys 
        515                 520                 525             
Ile Arg Gly Asn Lys Ile Lys Lys Arg Gln Arg Phe Ser Val Glu Leu 
    530                 535                 540                 
Lys Gln Glu Ile Tyr Asn Asp Leu Phe Cys Arg Asn Arg Lys Val Thr 
545                 550                 555                 560 
Gly Lys Lys Ile Leu Asp Tyr Leu Lys Arg Glu Tyr Asn Glu Ile Asp 
                565                 570                 575     
Gln Ala Asp Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ala Leu Ala 
            580                 585                 590         
Ser Tyr His Asp Phe Lys Lys Leu Val Gly Asn Gly Asn Ser Leu Asp 
        595                 600                 605             
Lys Glu Asp Tyr Lys Glu Ile Ile Glu Glu Ile Ile Lys Trp Lys Thr 
    610                 615                 620                 
Ile Tyr Asp Asp Asn Lys Met Leu Arg Arg Met Ile Lys Lys Tyr Pro 
625                 630                 635                 640 
Glu Val Leu Ser Glu Glu Gln Ile Lys Lys Ile Cys Lys Leu Lys Tyr 
                645                 650                 655     
Thr Gly Trp Gly Asn Phe Ser Arg Glu Phe Leu Thr Gly Val Arg Gly 
            660                 665                 670         
Lys Glu Thr Gly Glu Ser Ile Ile Asn Ala Leu Trp Glu Thr Asp Ser 
        675                 680                 685             
Ser Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Lys Asp Tyr Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Asp Gln Ile Asn Ala Glu Ala Ala Gly Glu Asn Ser 
705                 710                 715                 720 
Tyr Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Asn Lys Arg Ala 
                725                 730                 735     
Ile Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Lys His Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Gly Lys Glu 
        755                 760                 765             
Lys Asn Ala Lys Arg Thr Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu 
    770                 775                 780                 
Tyr Lys Ser Cys Lys Asn Asp Thr Arg Asn Leu Met Lys Glu Leu Gly 
785                 790                 795                 800 
Arg Asp Asp Asn Glu Leu Arg Ser Lys Lys Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Met Gly Lys Cys Met Tyr Ser Gly Glu Glu Ile Asp Leu Asp Asp 
            820                 825                 830         
Leu Leu Asn Trp Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Met Val Leu Val Arg Lys Glu Val Asn Ala 
    850                 855                 860                 
Lys Lys Ser Asn Glu Leu Leu Ser Pro Glu Ile Arg Glu Lys Met Lys 
865                 870                 875                 880 
Pro Tyr Trp Lys Met Leu Leu Gln Lys Gly Phe Ile Ser Lys Lys Lys 
                885                 890                 895     
Tyr Asp Arg Leu Thr Arg Thr Thr Arg Gly Glu Phe Thr Asp Glu Glu 
            900                 905                 910         
Leu Ser Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr 
        915                 920                 925             
Lys Ala Val Ala Glu Leu Leu Lys Gln Leu Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Arg Asp Ser Glu Val Val Tyr Val Lys Ala 
945                 950                 955                 960 
Asn Leu Val Ser Glu Phe Arg Gln Asp Phe Asp Leu Leu Lys Cys Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val 
            980                 985                 990         
Val Gly Asn Val Tyr His Val Arg Tyr Thr Asn Asn Pro Ala Lys Trp 
        995                 1000                1005            
Ile Lys Gly Asn Arg Asp Ser Asn Tyr Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Leu His Phe Tyr Ser 
1025                1030                1035                1040
Ser Ile Lys Lys Met Phe Ser Tyr Asp Val Lys Asn Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Lys Asn Gly Glu Ile 
            1060                1065                1070        
Val Trp Asn Lys Asp Ser Ser Ile Asp Gln Ile Arg Lys Ile Met Asn 
        1075                1080                1085            
Lys Asn Asn Ile Leu Tyr Thr Glu Tyr Thr Tyr Cys Glu Lys Gly Gln 
    1090                1095                1100                
Leu Phe Asn Glu Thr Leu Leu Lys Lys Gly Asn Ser Glu Lys Leu Ile 
1105                1110                1115                1120
Pro Leu Lys Lys Asn Leu Asp Pro Glu Lys Tyr Gly Gly Tyr Thr Thr 
                1125                1130                1135    
Pro Lys Ala Ser Tyr Phe Ala Leu Val Glu Phe Asp Ile Glu Glu Gly 
            1140                1145                1150        
Lys Lys Arg Lys Arg Lys Thr His Lys Gln Leu Ile Pro Val Pro Val 
        1155                1160                1165            
Tyr Glu Lys Ala Lys Leu Glu His Asn Ala Val Lys Tyr Leu Glu Glu 
    1170                1175                1180                
Arg Gly Leu Ser Asn Val Val Val Asp Leu Lys Tyr Lys Ile Lys Lys 
1185                1190                1195                1200
Asn Ser Leu Leu Lys Met Asp Gly Tyr Arg Met Arg Leu Arg Gly Ala 
                1205                1210                1215    
Asn Ile Ile Phe Lys Asn Ala Val Gln Leu Val Leu Asp Glu Glu His 
            1220                1225                1230        
Tyr Glu Ile Leu Arg Lys Val Glu Lys Tyr Leu Glu Lys Asn Ser Glu 
        1235                1240                1245            
Asn Gln Thr Ile Ala Lys Asn Glu Gly Phe Thr Glu Glu Leu Leu Ser 
    1250                1255                1260                
Leu Tyr Glu Glu Leu Leu Glu Lys Leu Lys Asp Tyr Lys Asn Arg Pro 
1265                1270                1275                1280
Ala Asn Gln Met Lys Thr Leu Glu Lys Gly Lys Glu Ala Phe Glu Ser 
                1285                1290                1295    
Ile Asn Leu Glu Asp Lys Ala Glu Val Ile Asn Glu Ile Ile Lys Leu 
            1300                1305                1310        
Phe Arg Cys Asn Ala Thr Thr Ala Ser Asn Ala Leu Lys Leu Ile Asn 
        1315                1320                1325            
Gly Ser Gln Asn Arg Met Lys Asn Lys Asn Ile Leu Asn Ser Asn Lys 
    1330                1335                1340                
Val Lys Leu Ile Asn Gln Ser Ile Thr Gly Leu Tyr Glu Thr Lys Ile 
1345                1350                1355                1360
Asp Leu Val Lys Leu Gly Glu Asp 
                1365            

<210> 127
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #66

<400> 127
Met Lys Lys Asp Tyr Tyr Ile Gly Leu Asp Met Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Cys Lys Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Glu Thr Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ala Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ser Glu Glu Ile Ser Lys Val Asp Ile Ala 
                85                  90                  95      
Phe Phe Leu Arg Leu Asn Glu Ser Lys Leu Tyr Pro Glu Asp Lys Thr 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Met Gln 
    130                 135                 140                 
Asn Asp Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Phe Glu Gly Asp Leu Asp Ser 
                165                 170                 175     
Glu Val Thr Asp Phe Gly Ala Val Phe Lys Glu Leu Leu Lys Val Ile 
            180                 185                 190         
Asn Asp Glu Leu Glu Ile Ala Leu Leu Ser Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Lys Ser Lys Ser Ala Lys Ser Lys Glu 
    210                 215                 220                 
Ile Val Lys Leu Leu Gly Ser Glu Lys Lys Gln Ala Cys Ile Lys Ala 
225                 230                 235                 240 
Leu Val Lys Leu Ile Val Gly Leu Thr Val Lys Leu Ser Glu Ile Phe 
                245                 250                 255     
Gly Asp Gly Glu Lys Ser Ser Ile Ser Phe Ala Glu Ser Asn Tyr Glu 
            260                 265                 270         
Glu Asn Val Glu Thr Val Glu Glu Thr Leu Pro Glu Arg Phe Ser Glu 
        275                 280                 285             
Leu Ile Glu Ala Ile Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Thr Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asp Leu Gln Gln Leu Lys 
                325                 330                 335     
Ala Leu Val Lys Thr His Cys Pro Glu Glu Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Leu Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Thr Leu Glu Glu Leu Glu 
    370                 375                 380                 
Lys Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Ser Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Ile Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Arg Gly Asn Gly Glu Asn Ala Trp 
    450                 455                 460                 
Met Val Arg Lys Glu Asp Gly Lys Ile Tyr Pro Trp Asn Phe Asp Asn 
465                 470                 475                 480 
Val Val Asp Val Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Glu Lys Ile Lys Lys Arg Gln Arg Phe Ser Val Glu Leu 
    530                 535                 540                 
Lys Gln Ala Ile Tyr Asn Asp Leu Phe Cys Arg Arg Arg Lys Val Thr 
545                 550                 555                 560 
Gly Lys Lys Leu Lys Asp Tyr Leu Lys Arg Glu Gly Gly Thr Gly Lys 
                565                 570                 575     
Glu Ala Glu Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ser Leu Arg 
            580                 585                 590         
Ala Tyr His Asp Phe Lys Lys Ile Val Gly Lys Gly Asn Ser Leu Asp 
        595                 600                 605             
Glu Glu Asp Asn Gln Asn Ile Lys Glu Glu Ile Ile Lys Ser Ile Thr 
    610                 615                 620                 
Ile Phe Asp Asp Lys Lys Met Leu Lys Lys Arg Leu Lys Lys Tyr Pro 
625                 630                 635                 640 
Glu Ile Leu Ser Glu Glu Gln Ile Lys Lys Ile Cys Lys Leu Lys Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Thr Glu Val Arg Asp 
            660                 665                 670         
Lys Glu Thr Gly Glu Asn Ile Ile Asn Ala Leu Trp Glu Thr Asp Ser 
        675                 680                 685             
Ser Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Lys Asp Tyr Ser Phe 
    690                 695                 700                 
Gln Glu Glu Ile Glu Gln Ile Asn Ala Glu Ala Ala Gly Glu Ser Ser 
705                 710                 715                 720 
Tyr Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Ala 
                725                 730                 735     
Ile Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Lys His Glu Pro Lys Arg Val Phe Val Glu Met Ala Arg Glu Lys Gly 
        755                 760                 765             
Lys Asn Gly Lys Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu 
    770                 775                 780                 
Tyr Lys Ser Cys Lys Asn Asp Ala Pro Asp Leu Trp Lys Glu Leu Gly 
785                 790                 795                 800 
Arg Asp Asp Asn Glu Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Met Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp 
            820                 825                 830         
Leu Phe Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Val Lys Lys Glu Ile Asn Ser 
    850                 855                 860                 
Lys Lys Ser Asp Asn Tyr Pro Ser Pro Glu Ile Arg Glu Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Met Leu Leu Lys Lys Gly Phe Ile Ser Lys Lys Lys 
                885                 890                 895     
Tyr Asp Arg Leu Thr Arg Thr Thr Arg Gly Glu Phe Thr Asp Asp Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr 
        915                 920                 925             
Lys Ala Val Ala Glu Leu Leu Lys Gln Leu Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Arg Asp Thr Glu Ile Val Tyr Val Lys Ala 
945                 950                 955                 960 
Asn Leu Val Ser Glu Phe Arg Gln Asp Phe Asp Phe Ile Lys Cys Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val 
            980                 985                 990         
Val Gly Asn Val Tyr His Val Lys Tyr Thr Lys Asn Ala Ala Lys Phe 
        995                 1000                1005            
Ile Lys Gly Asn Pro Gly Arg Asn Tyr Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Met His Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Lys Lys Met Phe Thr Ser Asp Val Pro Ser Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Arg Asn Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Asp Gly Ser Ile Ala Thr Val Arg Lys Ile Met Asn 
        1075                1080                1085            
Lys Asn Asn Ile Leu Tyr Thr Arg Tyr Ser Tyr Glu Glu Lys Gly Gly 
    1090                1095                1100                
Leu Phe Asp Gln Gln Ile Leu Lys Lys Gly Asn Gly Gln Ala Leu Ile 
1105                1110                1115                1120
Pro Leu Lys Lys Arg Leu Asp Ile Glu Lys Tyr Gly Gly Tyr Asn Lys 
                1125                1130                1135    
Pro Thr Ala Ala Tyr Phe Ala Leu Val Glu Tyr Asp Ile Glu Glu Gly 
            1140                1145                1150        
Lys Lys Gly Lys Lys Lys Thr Thr Lys Gln Leu Val Ala Ile Pro Ile 
        1155                1160                1165            
Tyr Glu Lys Ala Lys Leu Glu Glu Asn Pro Ile Lys Tyr Leu Glu Glu 
    1170                1175                1180                
Arg Gly Leu Lys Asn Pro Ala Val Asp Leu Leu Ile Lys Ile Lys Lys 
1185                1190                1195                1200
Asn Ser Leu Phe Glu Val Asp Gly Phe Arg Met His Leu Ser Gly Arg 
                1205                1210                1215    
Thr Ile Val Phe Lys Ser Ala Val Gln Leu Val Leu Asp Glu Glu Asn 
            1220                1225                1230        
Glu Ala Ile Leu Lys Lys Val Ala Lys Val Leu Glu Lys His Lys Lys 
        1235                1240                1245            
Asn Gln Lys Ile Ser Lys Lys Glu Gly Tyr Thr Glu Glu Leu Leu Ser 
    1250                1255                1260                
Leu Tyr Asp Ala Leu Leu Glu Lys Leu Lys Asp Tyr Lys Ile Arg Leu 
1265                1270                1275                1280
Ala Thr Gln Ala Lys Thr Leu Glu Asn Gly Lys Glu Lys Phe Glu Lys 
                1285                1290                1295    
Leu Ser Leu Glu Asp Lys Cys Gln Val Ile Asn Glu Ile Leu His Leu 
            1300                1305                1310        
Phe Gln Cys Asn Ala Thr Thr Ala Asn Asn Ala Leu Ser Leu Ile Gly 
        1315                1320                1325            
Gly Ala Lys Asn Ile Met Thr Asn Asn Asn Ile Ser Lys Cys Asn Lys 
    1330                1335                1340                
Val Ser Leu Ile Asn Gln Ser Ile Thr Gly Leu Tyr Glu Thr Lys Ile 
1345                1350                1355                1360
Asp Leu Lys Lys Ile Gly Glu Asp 
                1365            

<210> 128
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #67

<400> 128
Met Lys Lys Glu Tyr Tyr Val Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Ile Cys Arg Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Glu Thr Ala Asn Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ala Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Lys Val Asp Ile Gly 
                85                  90                  95      
Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro Glu Asp Lys Pro 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Asp Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Gln 
    130                 135                 140                 
Asn Glu Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Phe Glu Gly Asp Ile Ser Ser 
                165                 170                 175     
Glu Val Thr Asp Phe Gly Ala Ala Phe Ser Glu Phe Leu Lys Val Ile 
            180                 185                 190         
Asn Asp Glu Leu Glu Ile Ser Leu Leu Asp Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Lys Ser Lys Ser Ala Lys Lys Thr Glu 
    210                 215                 220                 
Leu Val Lys Leu Leu Ala Ala Glu Lys Lys Gln Lys Cys Glu Lys Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Ser Gly Cys Thr Val Lys Leu Ser Asp Ile Phe 
                245                 250                 255     
Gly Asp Gly Glu Arg Ser Lys Ile Ser Phe Ala Asp Ser Asn Tyr Glu 
            260                 265                 270         
Glu Tyr Ile Ala Thr Val Glu Glu Thr Leu Ala Glu Arg Phe Leu Glu 
        275                 280                 285             
Leu Ile Glu Ala Ala Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Thr Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Arg Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Ala Leu Val Lys Thr His Leu Pro Glu Glu Tyr Lys Asp Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Leu Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Lys Glu Asp Phe Tyr Ser Phe Leu Lys Lys Asn Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Ser Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Ile Phe Lys Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Lys Gly Asn Gly Glu Asn Ala Trp 
    450                 455                 460                 
Ala Val Arg Lys Gln Asp Gly Lys Ile Tyr Pro Trp Asn Phe Glu Asn 
465                 470                 475                 480 
Val Ile Asp Val Glu Glu Ser Ala Glu Lys Phe Ile Arg Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asp Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Glu Lys Ile Lys Lys Arg Gln Arg Phe Ser Val Glu Leu 
    530                 535                 540                 
Lys Gln Ala Val Tyr Glu Asp Val Phe Cys Arg Tyr Arg Lys Val Thr 
545                 550                 555                 560 
Gly Lys Lys Leu Lys Asp Tyr Leu Ile Arg Glu Gly Ile Thr Gly Lys 
                565                 570                 575     
Glu Ala Glu Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ser Leu Arg 
            580                 585                 590         
Ala Tyr His Asp Phe Lys Gln Ile Val Gly Lys Gly Lys Leu Leu Asp 
        595                 600                 605             
Val Glu Leu Thr Gln Asn Glu Lys Glu Asp Ile Ile Lys Asn Ile Thr 
    610                 615                 620                 
Ile Phe Asp Asp Lys Lys Met Leu Lys Lys Arg Leu Lys Lys Tyr Pro 
625                 630                 635                 640 
Glu Ile Leu Ser Glu Lys Gln Ile Lys Lys Ile Cys Ala Leu Lys Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Glu Glu Val Gln Ala 
            660                 665                 670         
Lys Glu Thr Gly Glu Asn Ile Ile Ser Ala Leu Trp Glu Thr Asp Ser 
        675                 680                 685             
Ser Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Lys Asp Tyr Gly Phe 
    690                 695                 700                 
Gln Glu Glu Ile Glu Gln Leu Asn Ala Glu Ala Ala Gly Lys Thr Ser 
705                 710                 715                 720 
Tyr Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Pro 
                725                 730                 735     
Ile Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Glu Lys Val Met Gly 
            740                 745                 750         
Lys Ser Lys Pro Lys Arg Val Phe Val Glu Met Ala Arg Glu Lys Gly 
        755                 760                 765             
Glu Asn Gly Arg Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu 
    770                 775                 780                 
Tyr Lys Ser Cys Lys Lys Glu Ala Pro Asp Leu Trp Lys Glu Leu Gly 
785                 790                 795                 800 
His Asp Asp His Lys Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Met Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp 
            820                 825                 830         
Leu Phe Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Met 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Val Lys Arg Glu Leu Asn Ala 
    850                 855                 860                 
Lys Lys Thr Asp Asn Tyr Pro Ser Ala Glu Ile Arg Glu Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Asp Met Leu Leu Lys Lys Gly Phe Ile Ser Lys Glu Lys 
                885                 890                 895     
Tyr Asp Arg Leu Thr Arg Ser Thr Arg Gly Glu Phe Thr Glu Asn Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr 
        915                 920                 925             
Lys Ala Val Ala Glu Leu Leu Lys Gln Ala Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Lys Glu Thr Glu Ile Val Tyr Val Lys Ala 
945                 950                 955                 960 
Asn Thr Val Ser Glu Phe Arg Gln Asp Phe Asp Phe Ile Lys Val Arg 
                965                 970                 975     
Asp Val Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val 
            980                 985                 990         
Val Gly Asn Thr Tyr Tyr Val Lys Phe Thr Lys Asn Ala Ala Trp Phe 
        995                 1000                1005            
Ile Lys Gly Asn Pro Gly Arg Thr Tyr Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Leu His Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Lys Lys Met Phe Thr Ser Asp Val Ala Ser Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Arg Asn Gly Glu Ile 
            1060                1065                1070        
Ala Trp Gly Lys Asp Gly Thr Ile Val Thr Val Arg Lys Val Met Asn 
        1075                1080                1085            
Lys Asn Asn Ile Leu Phe Thr Arg Arg Ser Tyr Glu Val Lys Gly Gly 
    1090                1095                1100                
Leu Phe Asp Gln Gln Ile Met Lys Lys Gly Lys Gly Gln Ala Leu Ile 
1105                1110                1115                1120
Pro Val Lys Glu Arg Leu Ser Ile Glu Lys Tyr Gly Gly Tyr Asn Lys 
                1125                1130                1135    
Ala Thr Gly Ala Tyr Phe Met Leu Val Glu Ser Asp Ile Glu Glu Gly 
            1140                1145                1150        
Lys Lys Gly Lys Lys Lys Thr Thr Lys Glu Leu Val Ala Ile Pro Leu 
        1155                1160                1165            
Tyr Glu Lys Ala Lys Leu Glu Asp Asn Ala Ile Arg Tyr Leu Glu Glu 
    1170                1175                1180                
Arg Gly Leu Lys Asn Pro Lys Val Asp Leu Leu Ile Lys Ile Lys Lys 
1185                1190                1195                1200
Asp Ser Leu Phe Glu Val Asp Gly Phe Arg Met His Leu Ser Gly Arg 
                1205                1210                1215    
Thr Leu Ile Phe Lys Cys Ala Asn Gln Leu Val Leu Asp Glu Glu Asn 
            1220                1225                1230        
Glu Lys Ile Leu Lys Lys Val Ala Lys Val Leu Glu Arg His Lys Lys 
        1235                1240                1245            
Asn Ile Lys Ile Ser Asp Lys Asp Gly Tyr Thr Glu Glu Met Leu Ile 
    1250                1255                1260                
Lys Tyr Glu Ala Phe Leu Asp Lys Leu Lys Asn Tyr Asn Ile Arg Leu 
1265                1270                1275                1280
Ser Thr Gln Ala Lys Thr Leu Glu Asn Gly Lys Glu Lys Phe Glu Lys 
                1285                1290                1295    
Leu Ser Leu Glu Asp Lys Cys Gln Val Ile Asn Glu Ile Leu His Leu 
            1300                1305                1310        
Phe Gln Cys Gln Ser Val Ala Ala Asn Asn Ala Leu Ser Met Ile Gly 
        1315                1320                1325            
Gly Ala Ala Asn Ile Leu Val Asn Asn Asn Ile Ser Lys Cys Asn Lys 
    1330                1335                1340                
Val Ser Ile Ile Asn Gln Ser Ile Thr Gly Leu Tyr Glu Gln Glu Ile 
1345                1350                1355                1360
Asp Leu Lys Lys Ile Gly Glu Asp 
                1365            

<210> 129
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #68

<400> 129
Met Lys Lys Glu Tyr Tyr Val Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Ser Tyr His Ile Cys Arg Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ala His Gly Lys Ala Leu Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Glu Thr Ala Asn Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ser Arg Arg Arg Leu Asp Arg Arg Lys Arg Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Lys Val Asp Pro Gly 
                85                  90                  95      
Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro Glu Asp Lys Pro 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Gln 
    130                 135                 140                 
Asp Glu Glu Pro Pro Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Met Ile Lys His Arg Gly His Phe Leu Phe Ala Gly Asp Ile Ser Asp 
                165                 170                 175     
Glu Val Thr Asp Phe Gly Ala Ala Phe Ser Glu Phe Leu Lys Ile Ile 
            180                 185                 190         
Lys Asp Glu Leu Asp Ile Ser Leu Leu Asp Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Leu Thr Arg Ser Ala Lys Lys Thr Glu 
    210                 215                 220                 
Leu Val Lys Gly Leu Lys Ala Glu Lys Lys Asn Gly Cys Glu Lys Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Ser Gly Cys Thr Val Lys Leu Ser Asp Ile Phe 
                245                 250                 255     
Gly Asp Gly Glu Arg Pro Lys Ile Ser Phe Ala Asp Ser Ser Tyr Glu 
            260                 265                 270         
Asp Tyr Ala Ala Val Val Glu Asp Glu Leu Gly Glu Arg Phe Ile Glu 
        275                 280                 285             
Ile Ile Glu Ala Ala Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Ser Ile Ser Glu Ala Lys Val Arg Thr Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Arg Asp Leu Lys Tyr Leu Lys 
                325                 330                 335     
Ala Leu Val Lys Thr Tyr Leu Pro Glu Glu Tyr Lys Asp Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Leu Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Lys Glu Asp Phe Tyr Asp Phe Leu Lys Lys Asn Ile Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Leu Glu Lys Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Tyr Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Ile Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Lys Gly Asn Gly Glu Phe Ala Trp 
    450                 455                 460                 
Ala Val Arg Lys Ser Asn Glu Lys Ile Tyr Pro Trp Asn Phe Glu Asn 
465                 470                 475                 480 
Val Ile Asp Val Glu Ala Ser Ala Glu Lys Phe Ile Arg Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asp Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Glu Lys Ile Lys Lys Arg Gln Arg Phe Ser Val Glu Leu 
    530                 535                 540                 
Lys Gln Glu Ile Tyr Glu Asp Val Phe Cys Arg Tyr Arg Lys Val Thr 
545                 550                 555                 560 
Ala Lys Lys Leu Lys Asp Tyr Leu Ile Arg Glu Gly Ile Thr Gly Lys 
                565                 570                 575     
Asp Ala Glu Ile Ser Gly Ile Asp Gly Asp Phe Lys Gly Ser Leu Arg 
            580                 585                 590         
Ala Tyr His Asp Phe Lys Glu Ile Leu Gly Lys Gly Lys Leu Leu Asp 
        595                 600                 605             
Val Glu Leu Thr Gln Asn Glu Lys Glu Asp Ile Ile Leu Asn Ile Thr 
    610                 615                 620                 
Leu Phe Asp Asp Lys Lys Leu Leu Lys Lys Arg Leu Lys Lys Tyr Pro 
625                 630                 635                 640 
Gln Ile Leu Thr Glu Lys Gln Ile Lys Lys Ile Cys Ala Leu Asn Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Glu Glu Val Gln Ala 
            660                 665                 670         
Pro Glu Thr Gly Glu Asn Ile Ile Thr Ala Leu Trp Glu Thr Asp Pro 
        675                 680                 685             
Ser Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Glu Asp Tyr Gly Phe 
    690                 695                 700                 
Gln Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Lys Thr Ser 
705                 710                 715                 720 
Tyr Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Gln 
                725                 730                 735     
Ile Trp Gln Thr Leu Gln Ile Val Lys Glu Ile Glu Lys Val Met Gly 
            740                 745                 750         
Lys Glu Lys Pro Lys Arg Val Phe Val Glu Met Ala Arg Glu Lys Gln 
        755                 760                 765             
Glu Asn Lys Arg Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Asp Leu 
    770                 775                 780                 
Tyr Lys Ser Cys Lys Asn Glu Glu Pro Asp Trp Trp Lys Glu Leu Gly 
785                 790                 795                 800 
His Glu Glu His Lys Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Lys Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp 
            820                 825                 830         
Leu Trp Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Met 
        835                 840                 845             
Asp Asp Ser Leu Asn Asn Arg Val Leu Val Lys Arg Glu Tyr Asn Ala 
    850                 855                 860                 
Lys Lys Thr Asp Asn Tyr Pro Ser Ala Glu Ile Arg Gln Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Lys Ser Leu Leu Thr Gly Gly Phe Ile Ser Lys Glu Lys 
                885                 890                 895     
Tyr Asp Arg Leu Ile Arg Ser Thr Arg Gly Glu Phe Thr Ala Asn Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Glu Arg Gln Leu Val Glu Thr Arg Gln Ser Thr 
        915                 920                 925             
Lys Ala Val Ala Glu Ile Leu Lys Gln Val Phe Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Lys Glu Thr Glu Ile Val Tyr Val Lys Ala 
945                 950                 955                 960 
Lys Thr Val Ser Glu Phe Arg Gln Asp Phe Asp Phe Ile Lys Val Arg 
                965                 970                 975     
Asp Val Asn Asp Phe His His Ala Lys Asp Ala Tyr Leu Asn Ile Val 
            980                 985                 990         
Val Gly Asn Thr Tyr Tyr Val Lys Phe Thr Lys Asn Ala Ala Trp Phe 
        995                 1000                1005            
Ile Lys Glu Asn Pro Gly Arg Thr Tyr Leu Lys Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Lys Lys Ala Asn Ala Lys Leu His Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Lys Lys Met Phe Thr Ser Asp Val Ala Val Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Lys Pro Ile Ile Glu Ile Asn Lys Arg Asn Gly Glu Ile 
            1060                1065                1070        
Ala Trp Gly Lys Ser Gly Thr Ile Val Thr Val Arg Lys Met Met Asn 
        1075                1080                1085            
Lys Asn Asn Ile Leu Phe Thr Arg Arg Ser Tyr Glu Val Lys Gly Gly 
    1090                1095                1100                
Leu Phe Asp Gln Gln Ile Met Lys Lys Gly Lys Gly Gln Val Tyr Ile 
1105                1110                1115                1120
Pro Val Lys Glu Arg Leu Ser Ile Glu Lys Tyr Gly Gly Tyr Asn Lys 
                1125                1130                1135    
Ala Thr Gly Ala Tyr Phe Met Leu Val Glu Ser Asp Ile Glu Glu Gly 
            1140                1145                1150        
Lys Lys Gly Lys Lys Lys Thr Thr Ile Glu Phe Val Pro Ile Pro Leu 
        1155                1160                1165            
Tyr Glu Lys Ala Lys Leu Glu Ala Asn Glu Ala Arg Phe Leu Glu Glu 
    1170                1175                1180                
Arg Gly Leu Lys Glu Pro Lys Val Asp Ile Leu Ile Lys Ile Lys Lys 
1185                1190                1195                1200
Asp Thr Leu Phe Glu Val Asp Gly Phe Lys Met His Leu Ser Gly Arg 
                1205                1210                1215    
Thr Leu Ile Phe Lys Cys Ala Asn Gln Leu Ile Leu Asp Glu Glu Asn 
            1220                1225                1230        
Glu Lys Ile Leu Lys Lys Val Ala Lys Val Ile Gln Arg Gln Lys Lys 
        1235                1240                1245            
Asn Val Lys Ile Ser Asp Lys Asp Gly Tyr Thr Glu Glu Leu Leu Met 
    1250                1255                1260                
Lys Tyr Glu Thr Phe Leu Asp Lys Leu Lys Asn Tyr Gly Ile Arg Leu 
1265                1270                1275                1280
Ser Thr Gln Ala Lys Thr Leu Glu Asn Gly Lys Glu Lys Phe Glu Lys 
                1285                1290                1295    
Leu Ser Leu Glu Asp Lys Cys Gln Val Leu Ser Glu Ile Leu His Leu 
            1300                1305                1310        
Phe Gln Cys Gln Ser Ser Ala Ala Asn Asn Ala Leu Ser Met Ile Gly 
        1315                1320                1325            
Gly Pro Gly Asn Ile Leu Val Asn Asn Asn Ile Ser Lys Cys Asn Lys 
    1330                1335                1340                
Ile Ser Ile Ile Asn Gln Ser Pro Thr Gly Ile Tyr Glu Gln Glu Ile 
1345                1350                1355                1360
Asp Leu Lys Lys Ile Gly Glu Asp 
                1365            

<210> 130
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #69

<400> 130
Met Glu Lys Glu Tyr Tyr Val Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Ser Tyr His Ile Leu Arg Arg His Met 
            20                  25                  30          
Lys Ile Leu Gly Asn Thr Glu Gly Lys Ala His Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Glu Ser Ala Lys Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ser Arg Arg Arg Leu Asp Arg Arg Asn Trp Arg Ile Glu 
65                  70                  75                  80  
Ile Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Lys Val Asp Pro Gly 
                85                  90                  95      
Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro Glu Asp Lys Pro 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Lys Asp Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Glu 
    130                 135                 140                 
Thr Glu Glu Thr Pro Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Met Met Lys His Arg Gly His Phe Leu Leu Ser Gly Asp Ile Ser Glu 
                165                 170                 175     
Glu Val Thr Glu Phe Gly Thr Thr Phe Ser Gln Phe Leu Glu Asn Ile 
            180                 185                 190         
Lys Asn Glu Leu Asp Phe Asn Leu Leu Asp Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Leu Thr Arg Ser Ala Lys Lys Thr Lys 
    210                 215                 220                 
Leu Val Lys Gly Leu Lys Ala Glu Lys Lys Asn Val Cys Glu Lys Ala 
225                 230                 235                 240 
Leu Leu Thr Leu Leu Ser Gly Gly Thr Val Lys Leu Ser Asp Ile Phe 
                245                 250                 255     
Gly Met Gly Glu Arg Pro Lys Ile Ser Phe Ala Asp Asn Gly Tyr Glu 
            260                 265                 270         
Asp Tyr Ala Ala Val Val Glu Asn Glu Leu Gly Glu Gln Phe Tyr Glu 
        275                 280                 285             
Ile Ile Glu Ala Ala Lys Ala Val Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Ser Val Ser Glu Ala Lys Val Arg Thr Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Lys His Lys Ser Asp Leu Lys Tyr Leu Lys 
                325                 330                 335     
Ala Ile Val Lys Lys Tyr Leu Pro Glu Glu Tyr Lys Asp Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Leu Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Lys Glu Glu Phe Tyr Asp Phe Leu Lys Lys Asn Ile Lys Glu Leu Glu 
    370                 375                 380                 
Ala Gln Glu Tyr Leu Gln Glu Glu Leu Glu Lys Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Asn Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Tyr Glu Leu Lys Lys Ile Leu Asp Asn Leu Gly Asp Lys Ile Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Leu Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Lys Gly Asn Gly Glu Phe Ala Trp 
    450                 455                 460                 
Ala Val Arg Lys Ser Asn Glu Lys Ile Tyr Pro Trp Asn Phe Glu Asn 
465                 470                 475                 480 
Val Ile Asp Val Glu Ala Ser Ala Glu Lys Phe Ile Arg Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Ile Gly Glu Asp Val Leu Pro Lys Asp Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Leu Asp Gly Glu Lys Ile Lys Lys Arg Gln Lys Phe Ser Val Glu Leu 
    530                 535                 540                 
Lys Gln Lys Ile Tyr Arg Asp Val Phe Cys Arg Tyr Arg Lys Val Thr 
545                 550                 555                 560 
Ala Lys Lys Leu Lys Asn Tyr Leu Ile Arg Glu Gly Ile Thr Gly Lys 
                565                 570                 575     
Asn Val Glu Ile Thr Gly Ile Asp Gly Asp Phe Lys Gly Ser Leu Thr 
            580                 585                 590         
Ala Tyr His Asp Phe Lys Glu Ile Leu Gly Lys Gly Lys Leu Leu Asp 
        595                 600                 605             
Val Glu Leu Thr Gln Asn Glu Lys Glu Glu Ile Ile Leu Asn Ile Thr 
    610                 615                 620                 
Leu Phe Asp Asp Lys Lys Leu Leu Lys Lys Arg Leu Lys Lys Tyr Pro 
625                 630                 635                 640 
Gln Ile Leu Thr Glu Lys Gln Ile Lys Lys Ile Cys Ala Leu Ser Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Lys Lys Phe Leu Glu Glu Val Thr Ala 
            660                 665                 670         
Pro Glu Thr Gly Glu Asn Ile Ile Thr Ala Leu Trp Glu Thr Asp Pro 
        675                 680                 685             
Ser Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Glu Asp Tyr Arg Phe 
    690                 695                 700                 
Met Glu Glu Ile Glu Gln Phe Asn Ala Glu Lys Ala Asp Lys Thr Ser 
705                 710                 715                 720 
Tyr Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Gln 
                725                 730                 735     
Ile Trp Gln Thr Leu Gln Ile Val Lys Glu Ile Glu Lys Val Met Gly 
            740                 745                 750         
Lys Glu Lys Pro Lys Arg Val Phe Val Glu Met Ala Arg Glu Lys Gln 
        755                 760                 765             
Glu Asn Lys Arg Gly Arg Glu Ser Arg Lys Lys Lys Leu Ser Asp Leu 
    770                 775                 780                 
Tyr Lys Ser Cys Lys Asn Glu Glu Pro Asp Trp Val Lys Glu Leu Gly 
785                 790                 795                 800 
His Glu Glu His Lys Leu Arg Ser Asp Lys Leu Tyr Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Lys Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp 
            820                 825                 830         
Leu Trp Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Thr Met 
        835                 840                 845             
Asp Asp Ser Leu Asn Asn Arg Val Leu Val Lys Lys Glu Tyr Asn Ala 
    850                 855                 860                 
Lys Lys Ser Asp Thr Tyr Pro Ser Ala Asp Ile Arg Gln Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Lys Ser Leu Leu Thr Gly Gly Phe Ile Ser Lys Glu Lys 
                885                 890                 895     
Tyr Asp Arg Leu Ile Arg Asn Asn Arg Gly Glu Leu Ser Ala Asn Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Glu Arg Gln Ile Val Glu Thr Arg Gln Ser Thr 
        915                 920                 925             
Lys Ala Val Ala Glu Ile Leu Lys Gln Val Phe Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Lys Glu Thr Glu Ile Val Tyr Val Lys Ala 
945                 950                 955                 960 
Lys Thr Val Ser Lys Phe Arg Gln Asp Phe Asp Phe Ile Lys Val Arg 
                965                 970                 975     
Asp Val Asn Asp Leu His His Ala Lys Asp Ala Tyr Leu Asn Ile Val 
            980                 985                 990         
Val Gly Asn Thr Tyr Tyr Val Lys Phe Thr Lys Asn Ala Ala Trp Phe 
        995                 1000                1005            
Ile Lys Glu Asn Pro Gly Arg Thr Tyr Leu Lys Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Lys Lys Ala Asn Ala Lys Leu His Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Lys Lys Met Phe Thr Ser Asp Val Ala Ile Glu Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Lys Pro Ile Ile Glu Ile Asn Lys Arg Asn Gly Glu Ile 
            1060                1065                1070        
Ala Trp Gly Lys Ser Gly Thr Ile Val Thr Val Arg Lys Met Met Asn 
        1075                1080                1085            
Lys Asn Asn Ile Leu Val Thr Arg Arg Ser Tyr Glu Val Lys Gly Gly 
    1090                1095                1100                
Leu Phe Asp Gln Gln Ile Met Lys Lys Gly Lys Gly Gln Val Tyr Val 
1105                1110                1115                1120
Pro Val Lys Glu Arg Leu Ser Ile Glu Lys Tyr Gly Gly Tyr Asn Lys 
                1125                1130                1135    
Ala Ala Gly Ala Tyr Phe Met Leu Val Glu Ser Asp Ile Glu Glu Gly 
            1140                1145                1150        
Lys Lys Gly Lys Glu Lys Arg Thr Ile Glu Phe Val Pro Ile Pro Leu 
        1155                1160                1165            
Tyr Asp Lys Ala Lys Leu Glu Ser Asp Glu Ala Arg Phe Leu Glu Glu 
    1170                1175                1180                
Arg Gly Leu Lys Glu Pro Lys Val Asp Ile Leu Ile Lys Lys Ile Lys 
1185                1190                1195                1200
Asp Thr Leu Phe Glu Val Asp Gly Phe Lys Met Trp Leu Ser Gly Arg 
                1205                1210                1215    
Thr Leu Ile Phe Lys Cys Ala Asn Gln Leu Ile Leu Asp Glu Glu Asn 
            1220                1225                1230        
Met Lys Thr Leu Lys Lys Val Val Lys Phe Ile Gln Arg Gln Lys Lys 
        1235                1240                1245            
Asn Val Lys Ile Ser Asp Lys Asp Gly Ile Thr Glu Glu Val Leu Met 
    1250                1255                1260                
Lys Tyr Glu Thr Phe Leu Asp Lys Leu Lys Asn Tyr Gly Ile Arg Leu 
1265                1270                1275                1280
Ser Ser Gln Ala Lys Thr Leu Val Asn Gly Lys Glu Lys Phe Glu Lys 
                1285                1290                1295    
Leu Ser Leu Glu Asp Lys Cys Gln Val Leu Tyr Glu Ile Leu His Leu 
            1300                1305                1310        
Phe Gln Cys Gln Ser Ser Ala Ala Asn Asn Ala Leu Lys Met Ile Gly 
        1315                1320                1325            
Gly Pro Gly Asn Ile Leu Val Asn Asn Asn Ile Ser Lys Cys Asn Lys 
    1330                1335                1340                
Ile Ser Ile Ile Asn Gln Ser Pro Thr Gly Ile Tyr Glu Lys Glu Ile 
1345                1350                1355                1360
Asp Leu Leu Lys Ile Gly Glu Asp 
                1365            

<210> 131
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #70

<400> 131
Met Lys Lys Asp Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Ser Ile Lys Lys Asn Leu Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Ser Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Asn Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Phe Val Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Glu Glu Gln His Leu Ser Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Lys Ser Arg Arg Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Leu Ile Lys Leu Ile Val Gly Leu Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Ala Met Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Asp Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Arg Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Val Thr Asp Arg Gly Lys Thr Gln Gln Asn Phe Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Glu Asn Tyr Leu Lys Lys Glu Tyr Glu Tyr Phe Asp 
                565                 570                 575     
Ser Pro Asp Ile Thr Gly Ile Glu Asp Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Ser Lys Asp Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Leu Thr Glu Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ser 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Val Ile Gly Glu Thr Glu Asn Leu 
705                 710                 715                 720 
His Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Arg Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Asn Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Glu Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu Asp Ser Arg Phe Asn Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Arg Lys Asn Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Thr Ala Lys Met His Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Ala Ser Glu Val Lys Ile Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Ile Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Leu Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Tyr Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Gln Glu Asp Val Leu Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile Asp Lys Ser Asn Ser Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Glu Glu His Arg Asn 
    1250                1255                1260                
Glu Phe Asp Glu Leu Leu Asp Tyr Ile Ile Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Gln 
                1285                1290                1295    
Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Met Gly Ala Pro Ala Ala Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Thr Ile Asp Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 132
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #71

<400> 132
Met Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Ser Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Asp Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Leu Phe Lys Gln Phe Val Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Leu Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Arg Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Ile Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Ala Met Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Thr Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Asp Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Arg Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Val Asp Asp Arg Gly Lys Thr Gln Gln Asn Phe Ser Ala Glu Glu 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Asn Tyr Ile Asp 
                565                 570                 575     
Ser Pro Thr Ile Lys Gly Ile Glu Asp Glu Phe Asn Ala Ser Phe Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Gly Val Ser Arg Glu Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ser 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ala Ile Asn Glu Thr Glu Asn Leu 
705                 710                 715                 720 
Gln Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Arg Tyr Gln Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu Asp Ser Arg Phe Asn Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Val Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Asn Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala His Glu Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ala Ser Glu Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Glu Lys Asp Ile Ala Thr Val Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Val Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Tyr Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asp Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Gln Met Val Leu Pro Ser His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile Ile Glu Ser Asn Ser Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Glu Glu His Arg Gln 
    1250                1255                1260                
Glu Phe Asp Glu Leu Leu Asp Tyr Ile Ile Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Gln 
                1285                1290                1295    
Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Arg Leu Gly Glu Leu Gly Glu Asp 
                1365            

<210> 133
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #72

<400> 133
Met Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Leu Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Lys Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Ala Met Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Asp Lys Tyr Tyr Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Asp Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Gln Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Lys Gln Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Asp 
                565                 570                 575     
Ser Pro Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Gly Val Ser Arg Glu Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ser Ile Asn Glu Thr Glu Asn Leu 
705                 710                 715                 720 
Gln Glu Ile Val Asn Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Arg Tyr Gln Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Ser Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ala Ser Glu Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Glu Lys Asp Ile Ala Thr Val Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Tyr Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asp Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Gln Met Val Leu Pro Ser His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Tyr Ile Glu Ser Asn Ser Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Gln 
    1250                1255                1260                
Glu Phe Asp Glu Leu Leu Asp Tyr Val Ile Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Gln 
                1285                1290                1295    
Asn Lys Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Arg Leu Gly Glu Leu Gly Glu Asp 
                1365            

<210> 134
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #73

<400> 134
Met Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Pro Met Ser Glu Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Thr Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Ile Ser Glu Thr Phe Glu Gln Phe Leu Asp Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Leu Val Asp Glu Thr Ile Lys Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Lys Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ile Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Gly Met Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Ala His Leu Pro Asp Lys Tyr Tyr Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Ser Lys Thr Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Asp Arg Gln Ser Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Val Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Lys Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Gln Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Ile Glu Ile Phe Asp Asp Leu Phe Lys Gln Lys Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Val Asn Tyr Leu Glu Asn Glu Tyr Gln Tyr Ile Asp 
                565                 570                 575     
Ser Pro Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Gly Val Ser Lys Glu Phe Leu Asp 
        595                 600                 605             
Asp Pro Glu Asn Glu Glu Ile Leu Glu Asp Ile Ile Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ser Ile Asn Glu Thr Glu Asn Leu 
705                 710                 715                 720 
Gln Glu Ile Val Asn Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Val Glu Ile Met Gly 
            740                 745                 750         
Arg Tyr Gln Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Asp Ser Asn Leu Leu Lys Glu Lys Pro 
785                 790                 795                 800 
Leu Asp Asn Glu Gln Leu Lys Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asn Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Leu Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Asn Val Asn Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Lys Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Asn Leu Lys Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Ser Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Met Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys His Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ser Ser Glu Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Glu Lys Asp Ile Ser Thr Val Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Arg Gln Met Asn Ile Val Lys Lys Val Glu Lys Gln Lys Gly Arg 
    1090                1095                1100                
Phe Tyr Lys Glu Thr Ile His Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Ile Lys Asn Asn Leu Asp Pro Lys Lys Tyr Gly Gly Phe Asp Glu 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Pro Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Thr Ala Tyr Leu Glu Lys 
    1170                1175                1180                
Thr Gly Tyr Asn Asn Pro Lys Glu Asp Val Leu Met Glu Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Gln Met Val Leu Pro Ser His Leu 
            1220                1225                1230        
Val Glu Leu Leu Tyr His Ala Lys Arg Tyr Ile Glu Ser Asn Ser Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Gln 
    1250                1255                1260                
Glu Phe Asp Glu Leu Leu Asp Tyr Val Ile Asp Phe Ser Asn Lys Tyr 
1265                1270                1275                1280
Ile Ser Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Lys Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Thr Ile Ser Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asp 
    1330                1335                1340                
Ser Thr Leu Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Arg Leu Glu Glu Leu Gly Glu Asp 
                1365            

<210> 135
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #74

<400> 135
Met Lys Lys Asn Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Lys Asp Asp Tyr Thr Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Gln Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Pro Met Ser Glu Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Asp Thr Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Ile Ser Glu Thr Phe Glu Gln Phe Leu Asp Thr Tyr 
            180                 185                 190         
Asn Glu Ile Phe Ser Glu Gln Glu Leu Val Asp Glu Ile Thr Lys Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Lys Lys Ala Glu Gln 
    210                 215                 220                 
Val Leu Ala Leu Phe Pro Asn Glu Lys Arg Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Asp Asp Ile Lys Leu Gln Leu Ser Glu Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Gly Met Ile Asp Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Asp Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Gln 
305                 310                 315                 320 
Met Ile Glu Leu Tyr Asp Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Ala His Leu Pro Asp Lys Tyr Tyr Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Val Leu Lys Lys Thr Asp 
    370                 375                 380                 
Asn Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Ser Phe Tyr Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Leu Arg Ala Ile Leu Asp Arg Gln Ser Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Arg Asp Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Glu Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Ile Val Asp Leu Glu Lys Ser Ala Glu Lys Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys Arg Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Glu Arg Gly Lys Thr Gln Gln Asn Phe Ser Ser Gln Glu 
    530                 535                 540                 
Lys Ile Glu Ile Phe Asp Asp Leu Phe Lys Gln Lys Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Val Asn Tyr Leu Glu Asn Glu Tyr Gln Tyr Ile Asp 
                565                 570                 575     
Ser Pro Asp Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Ala Lys Ile Gly Val Ser Lys Glu Tyr Leu Asp 
        595                 600                 605             
Asp Pro Glu Asn Glu Glu Ile Leu Glu Asp Ile Ile Lys Ile Leu Thr 
    610                 615                 620                 
Ile Phe Glu Asp Arg Lys Met Arg Lys Lys Gln Leu Gln Lys Tyr Ser 
625                 630                 635                 640 
Asp Thr Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Lys His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Glu Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Ser Phe 
    690                 695                 700                 
Lys Glu Lys Ile Glu Lys Ala Gln Thr Ile Asn Glu Thr Asn Asn Ile 
705                 710                 715                 720 
Gln Glu Ile Val Asn Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Ile Glu Ile Met Gly 
            740                 745                 750         
Arg Tyr Gln Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Lys Lys Gly Lys Asn Asn Ser Lys Ala Arg Leu Lys Lys Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Asp Ser Asn His Val Lys Glu Gln Pro 
785                 790                 795                 800 
Leu Asp Asn Glu Gln Leu Lys Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asn Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Lys Asp Ile Val Arg Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Lys Gln Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Arg Ala His Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Leu Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Asn Ile Asn Ile Val Leu Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Lys Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Asn Leu Lys Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Asn Ser Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Met Phe Lys Glu Asp Lys Ala Thr Ala Lys Lys His Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ser Lys Glu Lys Leu Val Asn Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Glu Lys Asp Leu Ser Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Lys Gln Met Asn Ile Val Lys Lys Val Glu Lys Gln Lys Gly Arg 
    1090                1095                1100                
Leu Phe Lys Glu Thr Ile His Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Ile Lys Asn Asn Leu Asp Pro Lys Lys Tyr Gly Gly Phe Ser Glu 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Pro Ile 
        1155                1160                1165            
Met Asp Arg Lys Ala Phe Glu Lys His Pro Thr Ala Tyr Leu Glu Lys 
    1170                1175                1180                
Thr Gly Tyr Asn Asn Pro Thr Glu Asp Val Leu His Glu Leu Ser Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Ser Arg Arg Met Ile Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Gln Met Val Leu Pro Ser His Leu 
            1220                1225                1230        
Val Glu Leu Leu Tyr His Ala Lys Arg Tyr Asp Glu Ser Asn Ser Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Ala 
    1250                1255                1260                
Lys Phe Asp Glu Leu Leu Asp Tyr Val Ile Asp Phe Ser Asn Lys Tyr 
1265                1270                1275                1280
Ile Asn Ala Asp Lys Asn Ile Glu Lys Ile Lys Lys Leu Tyr Lys Glu 
                1285                1290                1295    
Asn Lys Glu Ala Asp Val Lys Glu Leu Val Glu Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Thr Ile Ser Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Gly 
    1330                1335                1340                
Ser Thr Leu Ile Phe Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Arg Leu Glu Asp Leu Gly Glu Asp 
                1365            

<210> 136
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #75

<400> 136
Met Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Lys Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Tyr Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Asp Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Gln Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Lys Gln Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Glu 
                565                 570                 575     
Ser Pro Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Ile Gly Val Ser Arg Glu Val Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Leu Asp Glu Glu Ile Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ser Ile Thr Glu Thr Glu Asn Leu 
705                 710                 715                 720 
Gln Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Arg Tyr Gln Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Lys Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Glu Lys Leu Tyr Lys Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu His Gln Arg Phe Asn Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Ser Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ala Ser Glu Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Asp Ile Ala Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Tyr Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asp Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ser His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Tyr Ile Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Gln 
    1250                1255                1260                
Glu Phe Asp Glu Leu Leu Asp His Val Ile Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Gln 
                1285                1290                1295    
Asn Lys Glu Ala Asp Ile Lys Glu Leu Ala Gln Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Thr Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Arg Leu Gly Asp Leu Gly Glu Asp 
                1365            

<210> 137
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #76

<400> 137
Met Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Arg Met 
            20                  25                  30          
Lys Ile His Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Asp Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asn Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Asn Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Glu Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Asp Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ser Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Tyr Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Lys Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Tyr Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Asp Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Asn Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Glu Cys Gln Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asn Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Glu 
                565                 570                 575     
Ser Pro Thr Ile Lys Gly Ile Glu Asp Asn Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Ile Lys Ile Gly Val Ser Arg Glu Val Leu Asp 
        595                 600                 605             
Asp Glu Asp Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Leu Asp Glu Glu Ile Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Leu Thr Thr Glu Thr Glu Asn Leu 
705                 710                 715                 720 
Lys Asp Ile Val Asn Asn Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Arg Tyr Gln Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Gly Ser Asp Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Lys Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Lys Leu Tyr Lys Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu His Gln Arg Phe Asn Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Asp Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Ser Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Glu Phe Phe Ser Ser Glu Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Asp Ile Ala Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Ile Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Tyr Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asp Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ser His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Tyr Ile Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ala Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Gln 
    1250                1255                1260                
Gln Phe Asp Glu Leu Leu Asp His Val Ile Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Lys Glu Ala Asp Ile Lys Glu Leu Ala Gln Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Lys Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Lys Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 138
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #77

<400> 138
Met Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Arg Met 
            20                  25                  30          
Lys Ile Asn Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Lys Asp Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asn Glu Ser Phe Tyr Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Asn Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Glu Glu Thr Phe Lys Glu Phe Leu Gln Glu Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Leu Gln Pro Pro Val Asp Glu Asp Ile Asp Val 
        195                 200                 205             
Glu Ala Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Asn Leu Phe Pro Thr Glu Lys Ser Asn Gly Met Phe His Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ser Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Tyr Glu Ile Phe Arg 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Glu Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Ile Lys Lys Ile Asp Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Tyr Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Glu Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Lys Phe Ala Trp 
    450                 455                 460                 
Ile Glu Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Ser Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Glu Cys Gln Asn Phe Ser Ser Lys Glu 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asn Leu Phe Lys Lys Asn Arg Lys Val Ser 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Glu 
                565                 570                 575     
Ser Pro Thr Ile Lys Gly Ile Glu Asn Asn Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Ile Lys Ile Gly Val Ser Arg Glu Val Leu Asp 
        595                 600                 605             
Asn Glu Asp Tyr Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Leu Asp Glu Glu Ile Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Thr Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ala 
        675                 680                 685             
Leu Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Lys Glu Ile Glu Lys Ala Gln Leu Lys Thr Glu Ser Glu Asn Leu 
705                 710                 715                 720 
Lys Asp Ile Val Asp Asn Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Arg Tyr Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Gln Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Ile 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Gly Ser Asp Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Ser Lys Leu Tyr Lys Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Ser Ile Leu His Gln Arg Phe Asn Thr Glu Asn Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Asp Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Ser Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Glu Phe Phe Ser Ser Glu Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Asp Ile Ala Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Glu Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Ile Val Ile Thr His Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ile Phe Glu Lys Asn Gln Ile Ala Tyr Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr Val Asn Pro Lys Glu Asp Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Asn Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ser His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Tyr Ile Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ala Glu Ser Glu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg His 
    1250                1255                1260                
Gln Phe Asp Glu Leu Leu Asp His Val Ile Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Thr Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Lys Glu Ala Glu Ile Lys Glu Leu Ala Gln Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Thr Phe Asn Lys Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Asn Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Lys Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 139
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #78

<400> 139
Met Lys Lys Gln Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Ser Asp Asp Tyr Asp Leu Ile Arg Lys Arg Met 
            20                  25                  30          
Lys Ile Asn Gly Asp His Glu Lys Gly Ser Met Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Ile 
    50                  55                  60                  
Arg Arg Thr Thr Arg Arg Arg Leu Arg Arg Arg Arg Asn Arg Val His 
65                  70                  75                  80  
Tyr Leu Gln Ser Ile Phe Lys Asp Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asn Glu Ser Phe Tyr Val Pro Met Asp Lys Glu 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 
Ser Asn Glu Gln Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Glu Glu Thr Phe Lys Asp Phe Leu His His Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Leu Gln Pro Pro Ile Ser Glu Asp Ile Ser Val 
        195                 200                 205             
Glu Ala Asp Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Asn Lys Phe Gly Thr Glu Lys Ser Asn Gly Met Phe His Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Ser Leu Asp Glu Asp Arg Lys Leu Asn Ile Thr Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Glu Ile Gly Glu Asp Tyr Arg Glu 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Val His Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Val Ala Asp Asp Lys Thr Lys Ala Ser Leu Ser Ser Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Gln Asp Leu Ser Ala Leu Lys 
                325                 330                 335     
Asn Phe Ile Lys Ala His Leu Pro Asp Arg Tyr Tyr Asp Ile Phe Arg 
            340                 345                 350         
Asn Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Glu Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Val Leu Asp Lys Val Asp 
    370                 375                 380                 
Gly Ala Glu Ser Phe Ile Lys Lys Ile Asp Glu Glu Arg Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Tyr Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Glu Lys Gln Gly Gln Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Gln Ser Lys Phe Ala Trp 
    450                 455                 460                 
Ile Glu Arg Lys Gly Asp Glu Ser Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Glu Val Asp Leu Val Ser Ser Ala Glu Lys Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Ile Gln Lys Phe Asn Val Phe Asn Glu Leu Thr Lys Val Gln 
        515                 520                 525             
Tyr Val Asp Asp Gln Gly Lys Arg Cys Gln Asn Phe Ser Ser Lys Glu 
    530                 535                 540                 
Lys Gln Glu Ile Tyr Asp Asn Leu Phe Lys Lys Asn Arg Thr Val Ser 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asp Tyr Leu Lys Asn Glu Tyr Gln Tyr Leu Glu 
                565                 570                 575     
Asn Pro Thr Ile Lys Gly Ile Glu Asn Lys Phe Asn Ala Ser Leu Lys 
            580                 585                 590         
Thr Tyr His Asp Phe Ile Lys Leu Gly Val Ser Arg Asp Val Leu Asp 
        595                 600                 605             
Asn Glu Asn Tyr Glu Glu Arg Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Phe Ser 
625                 630                 635                 640 
Asp Val Leu Asp Gln Asp Ile Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Gln Lys Leu Ile Asp Gly Ile Arg Asp 
            660                 665                 670         
Glu His Thr Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ala 
        675                 680                 685             
Pro Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asp Leu Ser Phe 
    690                 695                 700                 
Lys Lys Thr Ile Glu Glu Ala Arg Leu Lys Thr Glu Ser Asp Asn Leu 
705                 710                 715                 720 
Lys Asp Ile Val Asp Lys Ile Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Glu Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Gln Gly Gln Arg Asn Ala Lys Glu Arg Leu Lys Arg Ile 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Lys Ser Asp Leu Leu Lys Lys His Pro 
785                 790                 795                 800 
Val Asp Gln Glu Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Asn Gln Glu Leu Asp Ile Asn Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Thr Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Asp Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Glu Glu Val Val Lys Asn Met Lys 
865                 870                 875                 880 
Pro Phe Trp Ser Ser Leu Tyr Arg Ser Asn Leu Ile Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Ser Ile Leu His Gln Arg Tyr Asn Thr Glu Asn Asp 
    930                 935                 940                 
Glu Glu Ala His Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Ala Phe Asp Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Asn Leu Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Asp Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Thr Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Ile Glu Arg Phe Ser His Glu Asp Gln Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Ser Lys Asp Ile Ala Lys Ile Lys Arg Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Thr Gly Asn 
    1090                1095                1100                
Phe Ser Lys Glu Thr Val Gln Pro Lys Gly Glu Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Ile Ile Phe Thr His Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Glu Val Lys Glu Ile Ile Gly Ile Thr Ile 
        1155                1160                1165            
Met Asp Gln Ser Ile Phe Glu Ala Asp Gln Ile Lys Tyr Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr Val Asn Pro Lys Glu Asp Ile Lys Phe Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Asn Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ser His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Cys Lys Arg Tyr Ile Asp Ser Ser Phe Asn 
        1235                1240                1245            
Ala Asp His Glu Glu Gln Lys Gln Thr Tyr Ile Asn Glu Asn Arg His 
    1250                1255                1260                
Gln Phe Asn Glu Leu Leu Asp His Val Leu Asn Phe Ser Ala Gln Tyr 
1265                1270                1275                1280
Thr Leu Ala Glu Ala Asn Glu Glu Lys Ile Lys Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asn Gln Ala Glu Val Ser Glu Leu Ala Thr Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Asp Phe Asn Lys Met Gly Ala Pro Ser Ala Phe Lys Phe Phe Gly 
        1315                1320                1325            
Thr Asn Ile Glu Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Lys 
1345                1350                1355                1360
Glu Val Gly Lys Leu Gly Glu Asp 
                1365            

<210> 140
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #79

<400> 140
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Asp Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Thr Lys Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu Asn Leu Pro Glu Lys Tyr His Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Asp Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Gln Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Lys Gln Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Ser Ala Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp 
        595                 600                 605             
Asp Pro Glu Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Ser Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Leu Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ser Ile Thr Asp Thr Glu Asn Leu 
705                 710                 715                 720 
Gln Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Arg Tyr Gln Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Arg Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Glu Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr His Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ala Ser Asp Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Asn Ile Ala Thr Val Lys Lys Val Leu Asn 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asp Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Arg Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asp Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys His Tyr Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Gln 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Glu His Val Ile Asp Phe Ala Glu Lys Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Gln Lys Leu Tyr Glu Gln 
                1285                1290                1295    
Asn Gln Glu Ala Asp Ile Lys Glu Leu Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Arg Leu Gly Asp Leu Gly Glu Asp 
                1365            

<210> 141
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #80

<400> 141
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Arg Leu Val Ala Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Ala Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Glu Leu Gln Glu Ile Phe Ala Glu Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      
Phe Phe Ala Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Ser Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Lys Ser Tyr 
        115                 120                 125             
His Gln Thr Tyr Pro Thr Ile Tyr His Leu Arg Gln His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Leu Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Arg Gln Phe Leu Gln Thr Tyr 
            180                 185                 190         
Asn Gln Gln Phe Ser Glu Pro Glu Pro Val Asp Glu Ser Val Asp Val 
        195                 200                 205             
Ala Val Ile Leu Thr Glu Lys Leu Ser Lys Thr Lys Lys Ala Glu Lys 
    210                 215                 220                 
Val Leu Lys Tyr Phe Pro Asn Glu Lys Ser Asn Gly Tyr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Met Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Glu Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Glu Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Gln Ala Lys Asn Val Tyr Asp Ala Ile Leu Leu Ser Glu 
    290                 295                 300                 
Ile Leu Ser Thr Thr Thr Lys Asn Thr Lys Ala Lys Leu Ser Ala Gly 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu Asn Leu Pro Glu Lys Tyr His Glu Leu Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Glu Leu Ser Gly Ile Asp 
    370                 375                 380                 
Gly Ser Glu Pro Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Thr Glu Leu Arg Ala Ile Ile Asp Arg Gln Lys Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Ala Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Gln Ser Pro Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Glu Glu Lys Ile Lys Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Met Glu Lys Ser Ala Met Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Met Ile Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Lys Asp Glu Arg Gly Lys Thr His Gln Tyr Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Arg Glu Ile Phe Asp Glu Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Gln Asn Phe Leu Tyr Leu Asn Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Asn Ala Glu Ile Lys Gly Ile Glu Lys Ala Phe Asn Ala Ser Tyr Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Leu Ser Glu Met Glu Glu Trp Leu Glu 
        595                 600                 605             
Asp Pro Glu Met Glu Ser Met Phe Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Glu Met Ile Arg Thr Gln Leu Ser Lys Tyr Glu 
625                 630                 635                 640 
Ala Val Leu Gly Glu Glu Ile Leu Lys Lys Leu Ala Lys Lys His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Gln Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly 
        675                 680                 685             
Phe Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ala Ile Thr Asp Thr Glu Asn Leu 
705                 710                 715                 720 
Gln Glu Ile Val Lys Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Leu Lys Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Asp Phe Gly Ser Gln Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Pro Leu Asp Ile His Arg 
            820                 825                 830         
Leu Ser Asp Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Lys Asp Asp Val Pro Ser Glu Lys Val Val Lys Lys Met Arg 
865                 870                 875                 880 
Pro Phe Trp Glu Lys Leu Tyr Arg Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ile Glu Arg Gly Gly Leu Thr Glu Glu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu His Gln Arg Phe Asn Ser Lys Lys Glu 
    930                 935                 940                 
Asp Glu Gly Lys Ala Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Ile Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Ile Ala Leu Leu Lys Lys Tyr Pro Gln Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Leu Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala His Lys Glu Asn Lys Ala Thr Ala Lys Lys Glu Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Glu Ser Asp Glu Pro Ile Cys Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Phe Trp Asp Lys Ser Lys Asn Ile Ala Gln Ile Lys Lys Val Ile Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Val Gln Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Ala Lys Tyr Gly Gly Phe Gly Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Thr Val Ala Phe Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Arg Lys Leu Lys Arg Ala Lys Ala Ile Glu Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Gln Asn Pro Ile Ala Phe Leu Lys Asn 
    1170                1175                1180                
Lys Gly Tyr Glu Asn Ala Lys Glu Asp Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Val Ala Ser 
                1205                1210                1215    
His Lys Glu Ala Gln Lys Ala Asn Ser Phe Ile Leu Pro Glu His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys His Tyr Asp Glu Ile Ser Phe His 
        1235                1240                1245            
Lys Glu Ser Phe Glu Gln Lys Gln Asp Tyr Val Asn Glu His Arg Ser 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Asp His Val Ile Asp Phe Ala Gly Lys Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Ile Glu Lys Ile Gln Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Gln Glu Asp Asp Ile Ala Glu Leu Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Asp 
        1315                1320                1325            
Val Thr Ile Pro Arg Lys Arg Tyr Thr Ser Leu Thr Glu Ile Trp Asp 
    1330                1335                1340                
Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Arg Met Gly Asn Leu Gly Glu Gln 
                1365            

<210> 142
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #81

<400> 142
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Arg Leu Val Ala Lys Lys Met 
            20                  25                  30          
Ser Val His Gly Asn Thr Glu Lys Lys Lys Val Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Ala Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Glu Leu Gln Glu Ile Phe Ala Glu Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      
Phe Phe Ala Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Thr Arg Phe Pro Ile Phe Pro Thr Leu Glu Glu Glu Lys Ser Tyr 
        115                 120                 125             
His Gln Thr Tyr Pro Thr Ile Tyr His Leu Arg Gln His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Leu Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Glu Leu Asp Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Arg Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Gln Phe Ser Glu Pro Glu Pro Val Asp Glu Ser Glu Gly Val 
        195                 200                 205             
Ala Val Ile Leu Thr Glu Lys Leu Ser Lys Thr Lys Lys Val Glu Lys 
    210                 215                 220                 
Val Leu Lys Tyr Tyr Pro Asn Glu Lys Ser Asn Gly Tyr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Glu Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Glu Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Gln Ala Lys Asn Val Tyr Asp Ala Ile Leu Leu Ser Glu 
    290                 295                 300                 
Ile Leu Ser Thr Thr Lys Lys Asn Thr Lys Ala Lys Leu Ser Ala Gly 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Lys Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu Asn Leu Pro Glu Lys Tyr Ala Glu Phe Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Glu Leu Ser Gly Ile Asp 
    370                 375                 380                 
Gly Ser Glu Pro Phe Leu Glu Lys Ile Asp Gln Glu Asn Phe Leu Leu 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Ala Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Thr Glu Leu Arg Ala Ile Ile Asp Arg Gln Lys Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Ala Gln Glu Lys Ile Glu Gln Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Gln Gly Gln Ser Pro Phe Ala Trp 
    450                 455                 460                 
Leu Glu Arg Lys Ser Ala Glu Lys Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Met Glu Lys Ser Ala Met Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Gln Asp Thr Tyr Leu Pro Thr Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Met Ile Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Lys Asp Glu Arg Gly Lys Thr His Gln Tyr Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Arg Glu Ile Phe Asp Glu Leu Phe Lys Lys His Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Gln Asn Phe Leu Tyr Leu Asn Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Asn Ala Glu Ile Lys Gly Ile Glu Lys Ala Phe Asn Ala Ser Tyr Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Leu Ala Glu Met Glu Glu Trp Leu Glu 
        595                 600                 605             
Asp Pro Glu Met Glu Ser Met Phe Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Ile Phe Glu Asp Arg Glu Met Ile Arg Thr Gln Leu Ser Lys Tyr Glu 
625                 630                 635                 640 
Ala Val Leu Gly Glu Gln Val Leu Lys Lys Leu Ala Lys Lys His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Glu Lys Leu Ile Gln Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Asp 
        675                 680                 685             
Phe Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp His Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Leu Ala Ile Ser Asp Ser Glu Ser Leu 
705                 710                 715                 720 
Leu Glu Val Val Lys Ala Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Trp Gln Ser Leu Lys Ile Val Glu Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Asn Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Leu Lys Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Asn Phe Gly Ser Pro Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Pro Leu Asp Ile His Arg 
            820                 825                 830         
Leu Ser Glu Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Ile Val 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Met Asp Asp Val Pro Ser Glu Lys Val Val Lys Lys Met Arg 
865                 870                 875                 880 
Pro Phe Trp Glu Lys Leu Gln Arg Ala Lys Leu Ile Ser Glu Arg Lys 
                885                 890                 895     
Phe Ala Asn Leu Thr Lys Ile Glu Arg Gly Gly Leu Thr Glu Glu Asp 
            900                 905                 910         
Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu His Gln His Phe Asn Gln Lys Lys Glu 
    930                 935                 940                 
Asp Glu Gly Lys Ala Ser Gly Lys Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Thr Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Ile Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn His His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Ile Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asn Tyr Ile Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Leu His Lys Glu Asn Lys Ala Thr Ala Lys Lys Glu Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Glu Ser Glu Glu Pro Ser Cys Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Phe Trp Asp Lys Ser Arg Asn Ile Ala Gln Ile Lys Lys Val Ile Ser 
        1075                1080                1085            
Ser His Gln Val Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Tyr Lys Glu Thr Val Asn Pro Lys Gly Asn Pro Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Ser Lys Tyr Gly Gly Phe Gly Ser 
                1125                1130                1135    
Pro Val Val Ala Tyr Thr Val Ala Phe Ile Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Arg Lys Leu Lys Arg Ala Lys Ala Ile Glu Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Gln Ser Ala Phe Glu Gln Asp Pro Ile Ala Phe Leu Lys Asn 
    1170                1175                1180                
Lys Gly Tyr Glu Asn Val Lys Glu Asp Glu Phe Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Val Ala Ser 
                1205                1210                1215    
His Lys Glu Ala Gln Lys Ala Asn Ser Phe Ile Leu Pro Glu His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Gln His Tyr Asp Glu Ile Ser Phe His 
        1235                1240                1245            
Lys Glu Ser Phe Glu Gln Lys Gln Asp Tyr Val Asn Glu His Arg Ser 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Asp His Val Ile Asp Phe Ala Gly Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Ile Glu Lys Ile Gln Lys Leu Tyr Glu Gln 
                1285                1290                1295    
Asn Gln Glu Asp Asp Ile Ala Glu Leu Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Asp 
        1315                1320                1325            
Val Thr Ile Pro Arg Lys Arg Tyr Thr Ser Leu Thr Glu Ile Trp Asp 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Arg Met Gly Asn Leu Gly Glu Gln 
                1365            

<210> 143
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #82

<400> 143
Met Ala Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Arg Leu Met Ala Lys Lys Met 
            20                  25                  30          
Ser Val His Gly Asn Thr Glu Lys Lys Lys Val Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Ala Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Glu Leu Gln Glu Ile Phe Ala Glu Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      
Phe Phe Val Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Gln 
            100                 105                 110         
Tyr Thr Arg Phe Pro Ile Phe Pro Thr Leu Glu Glu Glu Lys Ser Tyr 
        115                 120                 125             
Tyr Gln Thr Tyr Pro Thr Ile Tyr His Leu Arg Gln Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Leu Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Glu Leu Asp Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Glu Ile Tyr 
            180                 185                 190         
Asn Gln Gln Phe Asp Gln Pro Leu Ala Val Asp Glu Ser Glu Gly Val 
        195                 200                 205             
Ala Val Ile Leu Thr Glu Lys Leu Ser Lys Thr Lys Lys Val Glu Lys 
    210                 215                 220                 
Ile Leu Lys Tyr Tyr Pro Thr Glu Lys Thr Asn Gly Cys Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Glu Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ser Leu Leu Glu Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Gln Ala Lys Lys Val Tyr Asp Ala Ile Leu Leu Ser Glu 
    290                 295                 300                 
Ile Leu Ser Thr Thr Lys Lys Asn Thr Lys Ala Lys Leu Ser Ala Gly 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Ser His Lys Lys Asp Leu Ala Lys Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu Asn Leu Pro Glu Lys Thr Ala Val Phe Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Glu Leu Ser Gly Ile Val 
    370                 375                 380                 
Gly Ser Glu Pro Phe Leu Glu Lys Ile Asp Gln Glu Asn Phe Leu Leu 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Ala Asn Gly Val Ile Pro His Gln Val His Leu 
                405                 410                 415     
Thr Glu Leu Lys Ala Ile Ile Asp Arg Gln Lys Gln Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Glu Glu Ala Arg Asp Lys Ile Ile Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Gln Gly Gln Ser Ser Phe Ala Trp 
    450                 455                 460                 
Leu Glu Arg Lys Thr Ala Glu Lys Ile Thr Pro Trp Asn Phe Thr Glu 
465                 470                 475                 480 
Val Val Asp Ile Glu Lys Ser Ala Met Arg Phe Ile Gln Arg Met Thr 
                485                 490                 495     
Asn Gln Asp Thr Tyr Leu Pro Thr Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Tyr Met Ile Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Lys Asp Glu Arg Gly Ile Lys His Gln Tyr Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Arg Glu Ile Phe Lys Glu Leu Phe Gln Lys Gln Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Lys Leu Gln Asn Phe Leu Tyr Ala Asn Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Asn Ala Glu Ile Leu Gly Ile Glu Lys Ala Phe Asn Ala Ser Tyr Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Leu Ala Lys Met Gln Glu Trp Leu Glu 
        595                 600                 605             
Gln Pro Glu Met Glu Ser Met Phe Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Ile Phe Glu Asp Arg Glu Met Ile Arg Thr Gln Leu Ser Lys Tyr Gln 
625                 630                 635                 640 
Ala Val Leu Gly Glu Lys Leu Leu Lys Lys Leu Ala Lys Lys His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Glu Lys Leu Ile His Gly Ile Arg Asp 
            660                 665                 670         
Lys Lys Thr Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Asp 
        675                 680                 685             
Phe Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp His Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Leu Ala Leu Ser Asp Asn Gln Ser Leu 
705                 710                 715                 720 
Leu Glu Val Val Gln Ala Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Trp Gln Ser Leu Lys Ile Val Glu Glu Leu Ile Glu Ile Ile Gly 
            740                 745                 750         
Gly Tyr Asn Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Lys Thr Asn Arg Gly Leu Lys Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Asn Phe Gly Ser Pro Leu Leu Lys Glu Gln Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Pro Leu Asp Ile Asp Arg 
            820                 825                 830         
Leu Ser Glu Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Ile Val 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Met Asp Asp Val Pro Ser Glu Lys Val Val Lys Lys Met Arg 
865                 870                 875                 880 
Arg Phe Trp Glu Lys Leu Gln Arg Ala Lys Leu Ile Ser Glu Arg Lys 
                885                 890                 895     
Phe Ala Tyr Leu Thr Lys Ile Glu Arg Gly Gly Leu Thr Pro Glu Asp 
            900                 905                 910         
Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu His Gln His Phe Asn Gln Pro Gln Glu 
    930                 935                 940                 
Asp Glu Gly Lys Gly Cys Gly Lys Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Thr Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Ile Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn His His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Ile Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asn Tyr Ile Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Leu Ala Lys Glu Asn Lys Ala Thr Ala Lys Lys Glu Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Leu Lys Phe Phe Glu Ser Glu Glu Pro Ser Cys Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Phe Trp Asp Lys Ser Arg His Ile Pro Gln Ile Lys Lys Val Ile Ser 
        1075                1080                1085            
Ser His Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Lys Gly Gly 
    1090                1095                1100                
Phe Tyr Lys Glu Thr Val Asn Pro Lys Gly Lys Pro Asp Lys Leu Ile 
1105                1110                1115                1120
Gln Arg Lys Asn Asn Trp Asp Val Ser Lys Tyr Gly Gly Phe Gly Ser 
                1125                1130                1135    
Pro Val Val Ala Tyr Ala Val Ala Phe Ile Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Arg Lys Leu Lys Lys Ala Lys Ala Ile Glu Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Gln Ser Ala Phe Glu Gln Asp Pro Ile Ala Phe Leu Lys Asn 
    1170                1175                1180                
Lys Gly Tyr Ser Asn Val Thr Glu Asp Lys Phe Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Val Ala Ser 
                1205                1210                1215    
His Lys Glu Ala Gln Lys Ala Asn Ser Phe Ile Leu Pro Glu Gln Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Gln His Tyr Asp Glu Ile Ser Phe His 
        1235                1240                1245            
Lys Glu Ser Phe Glu Gln Lys Gln Asp Tyr Val Asn Asp His Leu Ser 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Asp Gln Val Ile Asp Phe Ala Gly Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Ile Glu Lys Ile Gln Glu Leu Tyr Glu Gln 
                1285                1290                1295    
Asn Gln Glu Gly Asp Thr Gln Asn Leu Ala Gln Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Asp 
        1315                1320                1325            
Val Thr Ile Pro Arg Lys Arg Tyr Thr Ser Leu Thr Glu Ile Trp Asp 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Arg Met Gly Asn Leu Gly Glu Gln 
                1365            

<210> 144
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #83

<400> 144
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val Tyr Gly Asn Thr Glu Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Thr Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Thr Lys Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Val Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Glu Asn Leu Pro Glu Lys Tyr His Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile His Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Gln Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Gln Gln Ile Phe Asp Asp Leu Phe Lys Gln Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Ser Ala Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp 
        595                 600                 605             
Asp Pro Glu Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Leu Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ser Thr Thr Asn Thr Glu Asp Leu 
705                 710                 715                 720 
Gln Glu Ile Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Arg Tyr Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Arg Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Glu Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Ala Phe Trp Glu Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr His Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ala Ser Asp Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Asn Ile Ala Thr Val Lys Lys Val Leu Asn 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asp Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Arg Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asp Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys His Cys Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Gly Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Ala 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Glu His Val Ile Asp Phe Ala Glu Lys Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Leu Lys Leu Tyr Glu Gln 
                1285                1290                1295    
Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Arg Leu Gly Asp Leu Gly Glu Asp 
                1365            

<210> 145
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #84

<400> 145
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Glu Asp Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val Tyr Gly Asn Thr Glu Lys Lys Tyr Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Thr Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Ile 
        195                 200                 205             
Glu Glu Ile Phe Thr Glu Lys Val Ser Arg Ala Lys Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Thr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Thr Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Glu Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Ala Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Lys Asn Leu Pro Glu Lys Tyr His Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Ser Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile His Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Leu Ala Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Met Leu Tyr Glu Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Gln Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Gln Gln Ile Phe Asp Asp Leu Phe Lys Gln Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Ser Ala Thr Ile Lys Gly Ile Glu Asn Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Ile Gly Ile Thr Gln Glu Val Leu Asp 
        595                 600                 605             
Asp Pro Glu Asn Glu Glu Met Phe Glu Glu Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Leu Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ser Glu Thr Asn Thr Glu Asp Leu 
705                 710                 715                 720 
His Glu Ile Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Arg Tyr Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Arg Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Glu Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Ala Phe Trp Glu Lys Leu Tyr Arg Ser Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr His Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ala Ser Asp Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Asn Ile Ala Thr Val Lys Lys Val Met Asn 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ser Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Arg Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asp Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys His Cys Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Gly Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Ala 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Glu His Val Lys Asp Phe Ala Glu Lys Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Leu Lys Leu Tyr Glu Gln 
                1285                1290                1295    
Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Arg Leu Gly Asp Leu Gly Glu Asp 
                1365            

<210> 146
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #85

<400> 146
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Glu Asn Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val Tyr Gly Asn Thr Glu Lys Lys Tyr Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Glu Lys Arg 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Glu Glu Thr Phe Lys Thr Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Ile 
        195                 200                 205             
Glu Glu Ile Phe Thr Glu Lys Val Ser Arg Ala Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Thr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Thr Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Glu Tyr Glu 
            260                 265                 270         
Glu Glu Leu Glu Ala Leu Leu Ala Ile Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Ala Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Lys Glu Leu Pro Glu Lys Tyr His Glu Ile Phe Arg 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Ser Val Ser 
        355                 360                 365             
Gln Ala Glu Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Pro 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile His Lys Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asp Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Leu Ala Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Met Leu Tyr Glu Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Gln Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Lys Gln Ile Phe Asp Glu Leu Phe Lys Gln Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Leu Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Asp Ala Thr Ile Lys Gly Ile Glu Asn Ser Phe Asn Ala Ser Tyr Gly 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Ile Gly Ile Thr Gln Glu Met Leu Asp 
        595                 600                 605             
Asp Pro Glu Asn Glu Glu Met Phe Glu Glu Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Phe Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Gln Ser Glu Thr Asn Thr Glu Asp Leu 
705                 710                 715                 720 
His Glu Ile Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Arg Tyr Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Arg Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Glu Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu Tyr Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Ile 
865                 870                 875                 880 
Ala Phe Trp Glu Lys Leu Lys Arg Ser Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Leu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala His Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Thr Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr His Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ala Ser Asp Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Asn Ile Ala Thr Val Lys Lys Val Met Asn 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ser Arg Lys Thr Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Arg Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asp Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Asn Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys His Cys Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Gly Lys Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Ala 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Glu Tyr Val Lys Asp Phe Ala Glu Lys Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Leu Lys Leu Tyr Glu Gln 
                1285                1290                1295    
Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Arg Leu Gly Asp Leu Gly Glu Asp 
                1365            

<210> 147
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #86

<400> 147
Met Asn Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Asp Glu Asn Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Ile Gln Gly Asn Gly Asp Lys Lys Lys Val Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Ala Gly Glu Thr Ala Glu Gly Thr Arg Met 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Gly Glu Met Asp Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Glu Lys Arg 
            100                 105                 110         
Asn Glu Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Lys Glu Thr Phe Lys Thr Phe Leu Gln Ala Tyr 
            180                 185                 190         
Asn Gln Ala Phe Thr Val Gln Glu Pro Val Asn Glu Thr Ile Asp Ile 
        195                 200                 205             
Gly Ser Leu Phe Gln Glu Arg Val Ser Arg Gln Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Thr Phe Met Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Thr Phe 
                245                 250                 255     
Gly Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Glu Tyr Glu 
            260                 265                 270         
Glu Glu Leu Glu Gly Leu Leu Ala Ile Val Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Ala Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Lys Asp Ile Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Leu Ile Lys Arg Tyr Glu Glu His Lys Thr Asp Leu Glu Gln Leu Lys 
                325                 330                 335     
Lys Phe Val Arg Lys Glu Leu Pro Glu Lys Tyr Tyr Glu Val Phe Arg 
            340                 345                 350         
Asp Ser Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Ser Val Ser 
        355                 360                 365             
Gln Ala Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Glu Lys Ile Pro 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Arg Lys Gln Ser Arg Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asp Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Thr Asp Gly Ser Ile Arg Pro Trp Asn Phe Thr Glu 
465                 470                 475                 480 
Val Val Asp Glu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Ser Tyr Leu Pro Gln Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Met Leu Tyr Glu Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Glu Thr Gln Gln Asn Phe Ser Ser Val Glu 
    530                 535                 540                 
Lys Lys Glu Ile Phe Asp Glu Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Tyr Ala Leu Leu Glu Asn Phe Leu Lys Asn Gln Tyr Met Tyr Ile Glu 
                565                 570                 575     
Thr Ala Glu Ile Lys Gly Ile Glu Lys Ser Phe Asn Ala Ser Tyr Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Gly Ile Ser Gln Glu Met Leu Asp 
        595                 600                 605             
Asp Pro Glu Asn Glu Glu Met Phe Glu Glu Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Lys Glu Gln Leu Ser Lys Tyr Ala 
625                 630                 635                 640 
Asp Tyr Phe Glu Ala Gly Val Leu Lys Lys Leu Val Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Lys Lys Thr Ile Leu Asp Tyr Leu Met Arg Asp Asp Asp 
        675                 680                 685             
Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Ser Ile Ala Gln Gln Gln Ser Glu Asp Gln Thr Glu Asp Leu 
705                 710                 715                 720 
His Glu Ile Val Lys Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Pro Pro Thr Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Arg Lys Lys Ser Lys Pro Arg Gln Lys Val Leu 
    770                 775                 780                 
Glu Glu Ala Met Lys Glu Phe Gly Ser Asn Leu Leu Lys Glu Tyr Pro 
785                 790                 795                 800 
Pro Asp Asn Ala Asn Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Lys Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Met Val Leu Val Ser Ser Thr Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Lys Glu Val Val Glu Lys Met Ile 
865                 870                 875                 880 
Gly Phe Trp Glu Lys Leu Lys Arg Ser Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Leu Asn Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala His Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu His Gln Lys Tyr Asn Ser Glu Asp Glu 
    930                 935                 940                 
Asp Glu Gly Asn Thr Ile Arg Thr Thr Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Gln Ile Tyr Gln Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Arg Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Gln Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Leu Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Asn Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Arg Phe Phe Ala Ser Asp Gln Pro Lys Val Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Gln Ser Lys Asn Ile Ala Met Val Lys Lys Val Met Asn 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Thr Glu Ile Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Gln Pro Lys Gly Asp Ser Ala Lys Leu Ile 
1105                1110                1115                1120
Ala Arg Lys Thr Glu Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Ile Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Arg Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Arg Gln Phe Glu Lys Asn Glu Val Glu Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Arg Glu Asp Val Leu Val Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Leu Glu Asn Gly Arg Arg Arg Leu Ile Ala Ser 
                1205                1210                1215    
Ala Ser Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Asn His Leu 
            1220                1225                1230        
Val Glu Leu Leu Tyr His Ala Lys His Cys Asp Asp Leu Asn Phe Asp 
        1235                1240                1245            
Gly Lys Ser Leu Glu Gln Lys Gln Ala Tyr Leu Asn Ala His Arg Ala 
    1250                1255                1260                
Glu Phe Asn Glu Leu Phe Glu Tyr Val Lys His Phe Ala Glu Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Leu Lys Lys Leu Ser Lys Leu Tyr Glu Gln 
                1285                1290                1295    
Asn Gln Glu Ala Asp Ile Lys Val Met Ala Gln Ser Phe Leu Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Asp 
        1315                1320                1325            
Thr Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Ile Leu Thr 
    1330                1335                1340                
Ala Thr Ile Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr His Trp 
1345                1350                1355                1360
Lys Leu Gly Glu Leu Gly Glu Asp 
                1365            

<210> 148
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #87

<400> 148
Met Lys Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val Tyr Gly Asn Thr Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Ile Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Thr Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ala Asn Gly Glu Pro Leu Asp Glu Ser Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Thr Lys Lys Ala Glu Lys 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Lys Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Ile Gln Phe Ala Lys Glu Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Val Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Ser 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Lys Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Glu Asn Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Gln Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Asn Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Gln Ala Ile Ile His Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Leu Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Gln Asn Phe Ser Gly Glu Glu 
    530                 535                 540                 
Lys Gln Gln Ile Phe Asp Asp Leu Phe Lys Gln Lys Arg Lys Val Lys 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Gln Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Ser Ala Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp 
        595                 600                 605             
Asp Pro Glu Asn Ala Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Phe Ser 
625                 630                 635                 640 
Asp Val Leu Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe 
    690                 695                 700                 
Lys Asn Glu Ile Glu Lys Ala Gln Ser Ser Thr Asn Asp Glu Asp Leu 
705                 710                 715                 720 
Gln Glu Ile Val Asn Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Arg Tyr Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Lys Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Arg Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Ala Phe Trp Glu Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Gln Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr His Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Thr 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ala Lys Asp Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Tyr Leu Ala Thr Ile Lys Lys Val Leu Asn 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Lys Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Arg Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Thr Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asp Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys His Cys Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Gly Glu Ser Leu Glu Gln Lys Gln Ala Tyr Val Glu Glu His Arg Ala 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Glu His Val Ile Asp Phe Ala Glu Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Ala Asn Leu Glu Lys Ile Asn Lys Leu Phe Glu Gln 
                1285                1290                1295    
Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Ser Phe Lys Phe Phe Glu 
        1315                1320                1325            
Lys Thr Ile Glu Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Arg Leu Gly Asp Leu Gly Glu Asp 
                1365            

<210> 149
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #88

<400> 149
Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          
Lys Val Ala Gly Asn Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  
Asn Arg Thr Thr Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Ala Asn 
                85                  90                  95      
Phe Phe Tyr Arg Leu Glu Asp Ser Phe Tyr Val Glu Ser Glu Lys Arg 
            100                 105                 110         
His Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Lys Leu Val Asn 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Lys Leu Asp Thr 
                165                 170                 175     
Lys Asn Thr Ser Val Asp Glu Val Phe Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         
Asn Gln Val Phe Ala Ser Asp Ile Arg Leu Glu Glu Asn Lys Asp Val 
        195                 200                 205             
Ala Lys Ile Leu Ser Glu Lys Val Thr Arg Arg Glu Lys Leu Glu Lys 
    210                 215                 220                 
Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser Thr Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Ile Ser Leu Ile Val Gly Ser Lys Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Asp Glu Lys Thr Asp Ile Glu Cys Ala Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Glu 
        275                 280                 285             
Ile Phe Val Ala Ala Lys Asn Ala Tyr Asn Ala Val Val Leu Ser Ser 
    290                 295                 300                 
Ile Ile Thr Val Thr Asp Thr Glu Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Asp Lys His Glu Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Ala Phe Ile Lys Val His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Asn 
            340                 345                 350         
Asp Val Glu Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Lys 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Met Lys Lys Met Leu Glu Asn Ile Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Ile Asp Lys Ile Glu Glu Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile Pro His Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Glu Ala Ile Leu His Gln Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Tyr Glu Lys Ile Lys Ser Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly Gln Ser Glu Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Lys Val Asp Phe Gly Lys Ser Ala Ile Asp Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Gln Gly Lys Thr His Gln Tyr Phe Ser Gly Gln Glu 
    530                 535                 540                 
Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Lys Arg Lys Val Lys 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Gln Phe Leu Arg Asn Met Ser Asn His Ile Glu 
                565                 570                 575     
Ser Pro Thr Ile Glu Gly Val Glu Asp Ser Phe Asn Ser Ser Phe Ala 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Val Gly Val Thr Gln Glu Ile Leu Asp 
        595                 600                 605             
Asn Pro Leu Asn Ala Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu Gln Gln Phe Ser 
625                 630                 635                 640 
Asp Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Ile Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe 
    690                 695                 700                 
Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Ala Asp Lys Asp Ile 
705                 710                 715                 720 
Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Gly Ile Met Gly 
            740                 745                 750         
Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gly Lys Gly Lys Asn Asn Ser Lys Pro Arg Phe Lys Ser Leu 
    770                 775                 780                 
Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Glu Leu Arg Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Ala Asn Arg 
    850                 855                 860                 
Glu Lys Gly Asp Asp Val Pro Ser Leu Glu Val Val Arg Lys Arg Lys 
865                 870                 875                 880 
Val Phe Trp Glu Lys Leu Tyr Gln Ala Asn Leu Met Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Cys Glu Lys Asp 
    930                 935                 940                 
Glu Asn Gly Asn Thr Ile Glu Gln Val Arg Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Asn Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr His Gln Phe Asp Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Trp Phe Lys Ala Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Thr 
1025                1030                1035                1040
Asn Ile Met Leu Phe Phe Ala Lys Lys Asp Arg Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Tyr Leu Asp Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Arg Gln Met Asn Ile Val Lys Lys Thr Glu Ile Gln Lys Gly Glu 
    1090                1095                1100                
Phe Ser Lys Ala Thr Ile Lys Pro Lys Gly Asn Ser Ser Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ala Asn Trp Asp Pro Met Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Met Ala Tyr Ala Val Val Ile Glu Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Ile Ile Lys Glu Lys Glu Ile Ile Arg Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Thr Ala Phe Glu Lys Asp Gln Lys Ala Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Arg Asn Pro Lys Glu Asp Val Leu Thr Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Cys Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Asn Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Asn His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Asn Cys Glu Ala Ser Asn Phe Asp 
        1235                1240                1245            
Gly Lys Ser Leu Glu Gln Lys Gln Ala Tyr Ile Glu Ser His Arg Glu 
    1250                1255                1260                
Met Phe Ala Glu Leu Leu Ala His Val Ser Glu Phe Ala Lys Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Ala Asn Leu Glu Lys Ile Asn Gln Leu Phe Glu Gln 
                1285                1290                1295    
Asn Lys Glu Gly Asp Ile Lys Val Ile Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Ala Phe Asn Ala Met Gly Ala Pro Ala Ser Phe Lys Phe Phe Glu 
        1315                1320                1325            
Thr Thr Ile Glu Arg Lys Arg Tyr Asn Asn Leu Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Ala Arg Lys 
1345                1350                1355                1360
Arg Leu Asp Asp Leu Gly Glu Asp 
                1365            

<210> 150
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #89

<400> 150
Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          
Lys Val Ala Gly Asn Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  
Asn Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Ile Asp Ala Asn 
                85                  90                  95      
Phe Phe Cys Arg Leu Asn Asp Ser Phe Tyr Val Asp Ser Glu Lys Arg 
            100                 105                 110         
Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
                165                 170                 175     
Lys Asn Thr Ser Val Asp Gly Val Tyr Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         
Asn Gln Val Phe Ala Ser Asn Ile Lys Leu Glu Glu Asn Lys Asp Val 
        195                 200                 205             
Ala Lys Ile Leu Ala Glu Lys Val Thr Arg Lys Glu Lys Leu Glu Arg 
    210                 215                 220                 
Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser Thr Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Ile Ser Leu Ile Val Gly Ser Lys Gly Asn Phe Gln Lys Val Phe 
                245                 250                 255     
Asp Leu Val Glu Lys Thr Asp Ile Glu Cys Ala Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Ile Ile Gly Asp Glu Tyr Ala Glu 
        275                 280                 285             
Leu Phe Val Ala Ala Lys Asn Ala Tyr Asn Ala Val Val Leu Ser Ser 
    290                 295                 300                 
Ile Ile Thr Val Thr Asp Thr Glu Thr Asn Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Asp Thr His Glu Lys Asp Leu Ser Glu Leu Lys 
                325                 330                 335     
Ala Phe Ile Lys Leu His Leu Pro Lys Gln Tyr Glu Glu Ile Phe Ser 
            340                 345                 350         
Asn Val Glu Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Lys 
        355                 360                 365             
Gln Ala Asp Phe Tyr Lys Tyr Met Lys Thr Met Leu Glu Asn Ile Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Ile Ala Lys Ile Glu Glu Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile Pro His Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Glu Ala Ile Leu His Gln Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Tyr Asp Lys Ile Lys Ser Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly Gln Ser Glu Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Lys Val Asp Phe Gly Lys Ser Ala Val Asp Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Gln Gly Lys Thr Asn Gln Tyr Phe Ser Gly Gln Glu 
    530                 535                 540                 
Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Lys Arg Lys Val Lys 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Gln Phe Leu Arg Asn Met Ser His His Ile Glu 
                565                 570                 575     
Ser Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn Ser Ser Tyr Ala 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Val Gly Ile Lys Gln Glu Ile Leu Asp 
        595                 600                 605             
Asn Pro Leu Asn Thr Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu Gln Gln Phe Ser 
625                 630                 635                 640 
Asp Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Val Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe 
    690                 695                 700                 
Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Ala Asp Lys Asp Ile 
705                 710                 715                 720 
Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Ser Ile Met Gly 
            740                 745                 750         
Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gly Lys Gly Lys Asn Asn Ser Arg Pro Arg Tyr Lys Ser Leu 
    770                 775                 780                 
Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Glu Leu Arg Asn Asn Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala Gly Asn Arg 
    850                 855                 860                 
Glu Lys Gly Asp Asp Val Pro Pro Leu Glu Ile Val Arg Lys Arg Lys 
865                 870                 875                 880 
Val Phe Trp Glu Lys Leu Tyr Gln Gly Asn Leu Met Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Tyr Glu Lys Asp 
    930                 935                 940                 
Asp His Gly Asn Thr Met Glu Gln Val Arg Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Asn Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr His Gln Phe Asp Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Trp Phe Lys Ala Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Thr 
1025                1030                1035                1040
Asn Ile Met Leu Phe Phe Ala Gln Lys Asp Arg Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Tyr Leu Asp Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Arg Gln Met Asn Ile Val Lys Lys Thr Glu Ile Gln Lys Gly Glu 
    1090                1095                1100                
Phe Ser Lys Ala Thr Ile Lys Pro Lys Gly Asn Ser Ser Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Thr Asn Trp Asp Pro Met Lys Tyr Gly Gly Leu Asp Ser 
                1125                1130                1135    
Pro Asn Met Ala Tyr Ala Val Val Ile Glu Tyr Asp Ile Ala Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Ile Val Phe Glu Lys Lys Ile Ile Arg Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Lys Ala Phe Glu Lys Asp Glu Lys Ala Phe Leu Glu Glu 
    1170                1175                1180                
Gln Gly Tyr Arg Gln Pro Lys Glu Asp Val Leu Thr Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Cys Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Asn Glu Ala Gln Lys Gly Asn Gln Gln Val Leu Pro Asn His Leu 
            1220                1225                1230        
Val Thr Leu Leu His His Ala Lys Asn Cys Glu Ala Ser Asn Phe Asp 
        1235                1240                1245            
Gly Lys Ser Leu Glu Gln Lys Gln Asp Tyr Ile Glu Ser Asn Arg Glu 
    1250                1255                1260                
Met Phe Ala Glu Leu Leu Ala His Val Ser Glu Phe Ala Lys Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Ala Asn Leu Asn Lys Ile Asn Gln Leu Phe Glu Gln 
                1285                1290                1295    
Asn Lys Glu Gly Asp Ile Lys Val Ile Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Ala Phe Asn Ala Met Gly Ala Pro Ala Ser Phe Lys Phe Phe Glu 
        1315                1320                1325            
Thr Thr Ile Glu Arg Lys Arg Tyr Asn Asn Leu Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Ala Arg Lys 
1345                1350                1355                1360
Arg Leu Asp Asp Leu Gly Glu Asp 
                1365            

<210> 151
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #90

<400> 151
Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          
Lys Val Ala Gly Asn Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  
Asn Arg Thr Thr Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Ala Glu Met Ser Glu Val Asp Ala Asn 
                85                  90                  95      
Phe Phe Tyr Arg Leu Glu Asp Ser Phe Tyr Ile Glu Ser Glu Lys Arg 
            100                 105                 110         
His Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Lys Leu Val Asn 
    130                 135                 140                 
Ser Ser Asp Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Lys Leu Asp Thr 
                165                 170                 175     
Lys Asn Thr Ser Val Asp Glu Val Phe Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         
Asn Gln Val Phe Ala Ser Asp Ile Arg Leu Glu Glu Asn Lys Glu Val 
        195                 200                 205             
Ala Lys Ile Leu Ser Glu Lys Leu Thr Arg Arg Glu Lys Leu Asp Lys 
    210                 215                 220                 
Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser Thr Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Ile Ser Leu Ile Ile Gly Ser Lys Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Asp Glu Lys Thr Asp Ile Glu Cys Ala Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Glu 
        275                 280                 285             
Ile Phe Val Ala Ala Lys Asn Ala Tyr Asn Ala Val Val Leu Ser Ser 
    290                 295                 300                 
Ile Ile Thr Val Thr Asp Thr Glu Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Asp Lys His Glu Lys Asp Leu Lys Arg Leu Lys 
                325                 330                 335     
Ala Phe Phe Lys Val His Leu Pro Glu Lys Phe Asn Glu Val Phe Asn 
            340                 345                 350         
Asp Val Glu Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Lys 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Met Lys Lys Met Leu Glu Asn Ile Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Ile Asp Gln Ile Glu Glu Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile Pro His Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Glu Ala Ile Leu His Gln Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asp Tyr Glu Lys Ile Lys Ser Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly Gln Ser Glu Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Lys Val Asp Phe Gly Lys Ser Ala Ile Asp Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn Glu Leu Thr Lys Ile Arg 
        515                 520                 525             
Tyr Ile Asp Asp Gln Gly Lys Thr His Gln Tyr Phe Ser Gly Gln Glu 
    530                 535                 540                 
Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Lys Arg Lys Val Lys 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Gln Phe Leu Tyr Asn Met Ile Asn His Ile Glu 
                565                 570                 575     
Ser Pro Thr Ile Glu Gly Val Glu Asp Ala Phe Asn Ser Ser Phe Ala 
            580                 585                 590         
Thr Tyr His Asp Leu Gln Lys Val Gly Val Thr Gln Glu Ile Leu Asp 
        595                 600                 605             
Asn Pro Leu Asn Ala Glu Met Leu Glu Glu Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu Gln Gln Phe Ser 
625                 630                 635                 640 
Asp Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Ile Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe 
    690                 695                 700                 
Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Ala Asp Lys Asp Ile 
705                 710                 715                 720 
Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Gly Ile Met Gly 
            740                 745                 750         
Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gly Lys Gly Lys Asn Asn Ser Lys Pro Arg Phe Thr Ser Leu 
    770                 775                 780                 
Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Gly Leu Lys Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Ala Asn Arg 
    850                 855                 860                 
Glu Lys Gly Asp Asn Val Pro Ser Leu Glu Val Val Arg Lys Arg Lys 
865                 870                 875                 880 
Val Phe Trp Glu Lys Leu Tyr Gln Ala Lys Leu Met Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Cys Lys Lys Asp 
    930                 935                 940                 
Glu Asn Gly Asn Val Ile Glu Gln Val Arg Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Asn Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr His Gln Phe Asp Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Trp Phe Lys Ala Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Thr 
1025                1030                1035                1040
Asn Ile Met Leu Phe Phe Ala Lys Lys Asp Arg Ile Ile Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Tyr Leu Asp Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Arg Gln Met Asn Ile Val Lys Lys Thr Glu Ile Gln Lys Gly Glu 
    1090                1095                1100                
Phe Ser Asn Ala Thr Val Asn Pro Lys Gly Asn Ser Ser Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ala Asp Trp Asp Pro Ile Lys Tyr Gly Gly Phe Asp Gly 
                1125                1130                1135    
Ser Asn Met Ala Tyr Ala Ile Val Ile Glu Tyr Asp Ile Glu Lys Arg 
            1140                1145                1150        
Lys Lys Lys Thr Val Ile Lys Glu Lys Glu Leu Ile Gln Ile Asn Ile 
        1155                1160                1165            
Met Glu Arg Thr Ala Phe Glu Lys Asp Gln Lys Ala Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Arg Asn Pro Lys Glu Asp Val Leu Thr Lys Ile Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Cys Glu Asn Gly Arg Arg Arg Met Leu Gly Ser 
                1205                1210                1215    
Ala Asn Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Asn His Leu 
            1220                1225                1230        
Met Thr Leu Leu Tyr His Ala Lys Asn Cys Glu Ala Ser Asn Phe Asp 
        1235                1240                1245            
Gly Lys Ser Leu Glu Gln Lys Gln Ala Tyr Ile Glu Ser His Arg Glu 
    1250                1255                1260                
Met Phe Ala Glu Leu Leu Ala His Ile Ser Glu Phe Ala Lys Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Ala Asn Leu Glu Lys Ile Asn Thr Leu Phe Glu Gln 
                1285                1290                1295    
Asn Lys Glu Gly Asp Ile Lys Val Ile Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Ala Phe Asn Ala Met Gly Ala Pro Ala Ser Phe Lys Phe Phe Glu 
        1315                1320                1325            
Thr Thr Ile Glu Arg Lys Arg Tyr Asn Asn Leu Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Ala Arg Lys 
1345                1350                1355                1360
Arg Leu Asp Asp Leu Gly Glu Asp 
                1365            

<210> 152
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #91

<400> 152
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Gln Phe Val Glu Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Glu Glu Ser His Leu Ser Glu Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Ala Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Asp Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Leu His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Arg Gly Lys Thr Gln Gln Phe Phe Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Lys Asp Tyr Leu Glu Lys Glu Phe Glu Glu Phe Asp 
                565                 570                 575     
Ser Val Asp Ile Thr Gly Val Glu Asp Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Leu Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu 
705                 710                 715                 720 
His Glu Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Arg Arg Asn Ser Arg Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Glu Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Lys Tyr Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Asp Arg Glu Arg Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Ile Gln Glu Asp Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile Asp Lys Ser Asn Gly Asn 
        1235                1240                1245            
Ser Glu Asn Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Lys Asn 
    1250                1255                1260                
Glu Phe Asp Glu Leu Leu Asp Tyr Ile Ile Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu Leu Tyr Asp Gln 
                1285                1290                1295    
Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 153
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #92

<400> 153
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe His Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asp Gln Leu Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Gln Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Asn Asp Glu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Gln Phe Leu Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Asp Ile Thr Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala 
625                 630                 635                 640 
Asp Leu Phe Asp Lys Lys Val Leu Lys Lys Leu Lys Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Val Ile Gly Gln Thr Asp Ser Leu 
705                 710                 715                 720 
His Glu Val Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ala Gln Gly Ile Lys Asn Ser Arg Gln Arg Met Lys Arg Leu 
    770                 775                 780                 
Glu Glu Val Leu Lys Lys Leu Gly Ser Asn Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Thr Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Lys Val Leu Thr Ser Ser Glu Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Tyr Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ser Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Pro Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Lys Tyr Asp Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Asp Lys Glu Arg Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asn Gly Thr 
                1045                1050                1055    
Ile Ile Lys Arg Pro Val Ile Glu Thr Asn Glu Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Gln Asp Ile Gln Glu Asp Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Phe Leu Tyr His Ala Ser Arg Ile Asp Lys Ser Thr Gly Ser 
        1235                1240                1245            
Ser Glu Asn Leu Glu Gln Lys Gln Glu Tyr Val Glu Gln His Lys His 
    1250                1255                1260                
Glu Phe Asp Glu Ile Leu Asp Tyr Ile Ile Asp Phe Ser Glu Arg Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ser Leu Tyr Asn Gln 
                1285                1290                1295    
Asn Asp Asp Ser Asp Ile Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Phe Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Ala Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 154
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #93

<400> 154
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
Tyr Glu Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe His Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asp Gln Leu Asp Thr Glu Ala Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Gln Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Asn Asp Glu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His His Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Glu Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Glu Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asn Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Glu Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Glu Phe Leu Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Asp Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Lys Arg Leu Ala Thr Tyr Ala 
625                 630                 635                 640 
Asp Leu Phe Asp Lys Lys Val Leu Lys Gln Leu Lys Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Val Ser Gly Gln Thr Asp Ser Leu 
705                 710                 715                 720 
His Glu Gln Ile Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ala Lys Gly Ile Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 
Glu Glu Val Leu Lys Lys Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Thr Gln Leu Gln Asn Glu Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asn Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Glu Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Ser Tyr Trp Gln Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ser Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Pro Ile Arg Asn Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gly Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Lys Tyr Asp Leu Arg Lys Met Ile Ala Lys 
    1010                1015                1020                
Ser Asp Lys Glu Arg Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asn Gly Thr 
                1045                1050                1055    
Ile Ile Lys Arg Pro Leu Ile Glu Thr Asn Glu Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Gln Asp Ile Gln Glu Asp Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Thr Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Phe Leu Tyr His Ala Ser Arg Ile Asp Lys Ser Thr Gly Ser 
        1235                1240                1245            
Ser Glu Asn Leu Glu Gln Lys Gln Glu Tyr Val Glu Gln His Lys His 
    1250                1255                1260                
Glu Phe Asp Glu Ile Leu Asp Tyr Ile Ile Asp Phe Ser Glu Arg Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ser Leu Phe Asn Gln 
                1285                1290                1295    
Asn Asp Asp Ser Asp Ile Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe Lys Phe Leu Asp 
        1315                1320                1325            
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 155
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #94

<400> 155
Met Glu Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Lys Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Ser Asp Val Asp Lys Leu Phe Tyr Gln Leu Val Gln Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Leu Asp Ala Ser Gly Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Gln Phe Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Ala Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Glu Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Glu Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 
His Glu Gln Ile Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Thr Lys Gly Leu Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         
Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ala Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Pro Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys 
    1010                1015                1020                
Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Arg Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asn Gly Glu 
                1045                1050                1055    
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Ser Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Gly Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Lys Asp Ile Gln Lys Asp Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Thr Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Phe Leu Tyr His Ala Ser Lys Ile Glu Lys Ser Thr Gly Ser 
        1235                1240                1245            
Ser Glu Asn Leu Glu Gln Lys Gln Ala Tyr Ile Glu Gln His Lys His 
    1250                1255                1260                
Glu Phe Asp Glu Ile Leu Glu Tyr Ile Ile Asp Phe Ser Glu Arg Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Ser Lys Val Lys Ser Leu Phe Asn Lys 
                1285                1290                1295    
His Glu Asp Ser Asp Ile Ser Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe Lys Phe Leu Asp 
        1315                1320                1325            
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 156
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #95

<400> 156
Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 
Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 
His Glu Gln Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         
Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 
Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys 
    1010                1015                1020                
Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Arg Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu 
                1045                1050                1055    
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Ser Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Lys Asp Val Gln Lys Asp Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Gly Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala Lys Leu 
            1220                1225                1230        
Val Thr Phe Leu Tyr His Ala Ser His Ile Glu Lys Ser Lys Gly Ser 
        1235                1240                1245            
Pro Glu Asn Asn Glu Gln Lys Gln Ala Tyr Ile Glu Gln His Lys His 
    1250                1255                1260                
Asp Leu Asp Glu Ile Leu Glu Tyr Ile Ser Glu Phe Ser Lys Arg Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Ser Lys Val Lys Ser Leu Phe Asn Lys 
                1285                1290                1295    
His Glu Asp Ser Ser Ile Ser Glu Leu Ala Ser Ser Ile Ile Asn Leu 
            1300                1305                1310        
Phe Thr Leu Thr Ser Leu Gly Ala Pro Ala Ala Phe Lys Phe Leu Asp 
        1315                1320                1325            
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 157
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #96

<400> 157
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Lys Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Glu Glu Ser Phe Leu Val Pro Glu Asp Lys Glu 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe His Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Leu Leu Phe Glu Glu Asp Pro Leu Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Gln Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Gly Leu Ser Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Ser Asp Glu Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Lys Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Gln Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Gln Phe Leu Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asn Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Asp Ile Thr Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Gln Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Lys Lys Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala 
625                 630                 635                 640 
Asp Leu Phe Asp Lys Ser Val Leu Lys Lys Leu Lys Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Ser Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Val Ile Gly Lys Thr Glu Ser Leu 
705                 710                 715                 720 
Lys Glu Val Val Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ala Gln Gly Ile Lys Asn Ser Arg Gln Arg Met Lys Lys Leu 
    770                 775                 780                 
Glu Glu Val Leu Lys Lys Leu Gly Ser Asn Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Thr Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Asp Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Lys Val Leu Thr Ser Gln Glu Ala Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Tyr Trp Gln Lys Leu Leu Lys Ala Gly Ala Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ser Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Leu Asp 
    930                 935                 940                 
Glu Asn Asn Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Lys Tyr Asp Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Asp Lys Glu Arg Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asn Gly Thr 
                1045                1050                1055    
Ile Ile Lys Arg Pro Val Ile Glu Thr Asn Thr Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Ile Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Gln Asp Ile Gln Glu His Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Leu Val Leu Pro Ala His Tyr 
            1220                1225                1230        
Val Thr Phe Leu Tyr Leu Ala Ser Arg Tyr Asp Lys Leu Thr Gly Ser 
        1235                1240                1245            
Ser Glu Asp Leu Glu Gln Lys Lys His Phe Val Glu Gln His Leu His 
    1250                1255                1260                
Tyr Phe Asp Glu Ile Leu Asp Ile Ile Ile Asp Phe Ser Glu Arg Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Ser Asn Leu Glu Lys Ile Lys Ser Leu Tyr Asn Gln 
                1285                1290                1295    
Asn Asp Asp Phe Ser Ile Asn Glu Gln Ala Ser Asn Met Ile Asn Leu 
            1300                1305                1310        
Phe Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Ala Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 158
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #97

<400> 158
Met Arg Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Lys Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Asn Ile Phe Ser Asp Glu Met Ala Lys Val Asp Asp Ala 
                85                  90                  95      
Phe Phe Gln Arg Leu Ser Glu Ser Phe Leu Val Pro Glu Asp Lys Glu 
            100                 105                 110         
Phe Asp Arg His Pro Ile Phe Gly Asn Leu Lys Asp Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Asp Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Leu Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Leu Leu Phe Glu Glu Asp Pro Ile Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Thr Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Glu Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Gly Leu Ser Glu Asp Ala Lys Leu Gln Ile Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Ser Asp Glu Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Val Lys Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Lys Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asn Lys Glu Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Lys Glu Leu His Ala Ile Leu Arg Arg Gln Glu Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Ser Phe Ala Trp 
    450                 455                 460                 
Leu Lys Arg Arg Ser Asp Glu Ser Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Gln Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Pro Leu Tyr Glu Met Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Gln Phe Leu Ser Ala Glu Gln 
    530                 535                 540                 
Lys Glu Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asn Tyr Phe Ser Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Asp Ile Thr Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Ala Phe Leu Asp 
        595                 600                 605             
Asp Glu Ala Asn Gln Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Glu Lys Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala 
625                 630                 635                 640 
Asp Leu Phe Asp Lys Ser Val Leu Lys Lys Leu Lys Lys Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Ser Leu Asp Phe 
    690                 695                 700                 
Ala Lys Ile Ile Glu Asn Ala Gln Glu Lys Ala Lys Ser Glu Ser Leu 
705                 710                 715                 720 
Lys Glu Thr Val Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Arg His Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ala Gln Gly Ile Lys Asn Ser Arg Gln Arg Leu Lys Lys Leu 
    770                 775                 780                 
Glu Glu Val His Lys Lys Thr Gly Ser Lys Ile Leu Lys Glu His Ser 
785                 790                 795                 800 
Val Ser Asn Thr Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Leu Leu 
                805                 810                 815     
Gln Asp Gly Lys Asp Met Tyr Thr Gly Gln Asp Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Ile Val Leu Thr Ser Gln Glu Ala Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Tyr Trp Gln Lys Gln Leu Lys Ser Gly Ala Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp His Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ser Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Ser Lys Leu Asp 
    930                 935                 940                 
Glu Asn Asn Lys Ser Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Met Val Ser Asp Phe Arg Lys Asp Phe Gly Leu Tyr Lys Leu Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Ala Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys His Tyr Asp Leu Ala Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Asp Ser Ser Leu Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Lys Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Lys Arg Pro Val Ile Glu Thr Asn Thr Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Val Lys Asp Phe Lys Thr Ile Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Ile Ala Tyr Ser Val Leu Val Val Ala Lys Val Ala Lys Gly 
            1140                1145                1150        
Lys Ala Gln Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Gln Ser Glu Phe Glu Lys Asp Pro Ile Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asp Ile Gln Glu Asn Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Asn Lys Tyr 
            1220                1225                1230        
Val Lys Phe Leu Tyr Leu Ala Ser Arg Tyr Asp Lys Leu Thr Gly Lys 
        1235                1240                1245            
Ser Glu Asp Arg Glu Gln Lys Lys His Phe Val Glu Gln His Leu His 
    1250                1255                1260                
Tyr Phe Asp Glu Ile Leu Asp Ile Ile Val Asp Phe Ser Asn Arg Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Ser Asn Leu Glu Lys Ile Lys Ser Leu Tyr Asn Glu 
                1285                1290                1295    
Lys Asp Asp Phe Ser Ile Asn Glu Gln Ala Thr Asn Met Leu Asn Leu 
            1300                1305                1310        
Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Ala Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Asn Glu Val Leu Asn 
    1330                1335                1340                
Ser Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Gly Asp 
                1365            

<210> 159
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #98

<400> 159
Met Arg Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Lys 
65                  70                  75                  80  
Tyr Leu Gln Asn Ile Phe Ser Asp Glu Met Ser Lys Val Asp Ser Ala 
                85                  90                  95      
Phe Phe Gln Arg Leu Ser Glu Ser Phe Leu Val Pro Asp Asp Lys Glu 
            100                 105                 110         
Phe Asp Arg His Pro Ile Phe Gly Asn Leu Lys Asp Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Asp Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asp Ser 
                165                 170                 175     
Glu Asn Thr Asp Val His Ala Leu Phe Leu Lys Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Leu Leu Phe Glu Glu Asp Lys Ile Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Thr Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Glu Ile Pro Asn Gln Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ser Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Glu Leu Ser Glu Asp Ala Lys Leu Gln Ile Ser Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Ile Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Lys Gly Ala Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Val Gln Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Lys Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Asp 
            340                 345                 350         
Asn Lys Glu Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Lys Leu Ile Ser Lys Leu Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Asn Glu Leu Lys Ala Ile Ile Arg Arg Gln Glu Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Leu Phe Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Ser Phe Ala Trp 
    450                 455                 460                 
Leu Lys Arg Arg Ser Asn Glu Ser Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Gln Glu Ala Ser Ala Arg Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Pro Leu Tyr Glu Met Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Gln Thr Glu Gly Met Lys Lys Pro Glu Phe Leu Ser Ser Glu Glu 
    530                 535                 540                 
Lys Glu Glu Ile Val Asn Leu Leu Phe Lys Lys Glu Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asn Tyr Phe Ser Lys Ile Lys Cys Phe Asp 
                565                 570                 575     
Ser Val Thr Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Ala Phe Leu Asp 
        595                 600                 605             
Asp Glu Ala Asn Gln Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Glu Lys Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala 
625                 630                 635                 640 
Asp Val Phe Asp Lys Ser Val Leu Lys Lys Leu Lys Lys Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Gln Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Thr Gly Lys Thr Ile Leu Asp Phe Leu Lys Asp Asp Gly Val 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Ser Leu Asp Phe 
    690                 695                 700                 
Ala Lys Ile Ile Lys Asn Ala Gln Glu Lys Thr Lys Ser Glu Ser Leu 
705                 710                 715                 720 
Glu Glu Thr Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Arg His Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Ser Thr Ala Gln Gly Ile Lys Asn Ser Arg Gln Arg Leu Arg Lys Leu 
    770                 775                 780                 
Glu Glu Val His Lys Asn Thr Gly Ser Lys Ile Leu Lys Glu His Asn 
785                 790                 795                 800 
Val Ser Asn Thr Gln Leu Gln Ser Asp Arg Leu Tyr Leu Tyr Leu Leu 
                805                 810                 815     
Gln Asp Gly Lys Asp Met Tyr Thr Gly Lys Asp Leu Asp Phe Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Ile Val Leu Thr Ser Gln Glu Ser Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Ile Val Asn Lys Met Lys 
865                 870                 875                 880 
Ser Tyr Trp Gln Lys Gln Leu Lys Ser Gly Ala Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp His Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Phe Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Ser Asn Leu Asp 
    930                 935                 940                 
Glu Asn Ser Lys Ser Asn Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Met Val Ser Asp Phe Arg Lys Asp Phe Gly Phe Tyr Lys Leu Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Ala Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys His Tyr Asp Leu Ala Lys Leu Ile Val Lys 
    1010                1015                1020                
Ser Asp Ser Ser Leu Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Lys Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Thr Arg Pro Gln Ile Glu Thr Asn Thr Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Val Lys Asp Ile Lys Thr Ile Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Ile Ile Ala Tyr Ser Val Leu Val Val Ala Lys Val Ala Lys Gly 
            1140                1145                1150        
Lys Ala Gln Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Gln Asn Glu Phe Glu Lys Asp Pro Ile Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asp Ile Gln Glu Ser Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Asn Lys Tyr 
            1220                1225                1230        
Val Lys Phe Leu Tyr Leu Ala Ser Arg Tyr Thr Lys Phe Thr Gly Lys 
        1235                1240                1245            
Glu Glu Asp Arg Glu Lys Lys Arg His Phe Val Glu Ser His Leu His 
    1250                1255                1260                
Tyr Phe Asp Glu Ile Met Asp Ile Ile Val Asp Phe Ser Asn Arg Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Ser Asn Leu Glu Lys Ile Gln Asn Leu Tyr Asn Glu 
                1285                1290                1295    
Lys Asp Asn Phe Ser Ile Glu Glu Gln Ala Thr Asn Met Leu Asn Leu 
            1300                1305                1310        
Phe Thr Phe Thr Gly Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asn 
        1315                1320                1325            
Ala Asp Ile Asp Arg Lys Arg Tyr Thr Ser Thr Asn Glu Ile Leu Asn 
    1330                1335                1340                
Ser Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Gly Asp 
                1365            

<210> 160
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #99

<400> 160
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Glu Ser His Leu Ser Glu Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu Asn Leu Pro Asp Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Lys Thr Gln Gln Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Leu Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Leu Asp Tyr Leu Glu Lys Glu Phe Glu Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu 
705                 710                 715                 720 
Lys Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Asp Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Lys Tyr Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Val Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Ile Gln Glu Asp Asn Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile Asp Lys Ser Asn Gly Glu 
        1235                1240                1245            
Pro Glu His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Lys Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu Leu Tyr Asp Gln 
                1285                1290                1295    
Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 161
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #100

<400> 161
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Ile Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Glu Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asn Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu Asn Val Pro Asp Gln Tyr Asn Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Ser Tyr Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Ser Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ala Gln Gln Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp 
        595                 600                 605             
Asp Glu Ala Asn Glu Glu Ile Ile Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Val Asp Asp Leu 
705                 710                 715                 720 
Ala Gln Val Val His Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Asn His Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Gln Asp Ser Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu Lys Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Ala Arg Lys 
865                 870                 875                 880 
Ser Phe Trp Val Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Val Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Lys Lys Tyr Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser His Arg Glu Arg Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Val Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr His Asn Ile Gln Glu Asp Lys Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Gln Arg Ile Asp Lys Ser Asn Gly Glu 
        1235                1240                1245            
Pro Glu His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Lys Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Val Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ala Leu Tyr Asp Gln 
                1285                1290                1295    
Asn Asp Asp Ala Asp Ile Glu Glu Ile Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Cys Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 162
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #101

<400> 162
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Val Asp Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Ile Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Glu Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Val Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Val Asn Ala Pro Asp Gln Tyr Asn Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ala Lys Gln Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp 
        595                 600                 605             
Asp Lys Ala Asn Glu Glu Ile Ile Glu Asp Ile Ile Gln Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Gln Arg Leu Gln Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Asp Val Asp Asp Ile 
705                 710                 715                 720 
Ala Asn Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Asn His Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Gln Asp Ser Leu Lys Asn Leu Gly Ser Asn Ile Asn Lys Lys Lys Pro 
785                 790                 795                 800 
Val Glu Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Ile Glu Val Val Arg Ala Arg Lys 
865                 870                 875                 880 
Ser Phe Trp Val Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Val Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Lys Lys Tyr Asn Val Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser His Arg Glu Arg Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asn Lys Gln Lys Asp Phe Glu Ile Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr His Asn Ile Gln Glu Asp Lys Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala His Arg Ile Asp Lys Ser Asn Gly Glu 
        1235                1240                1245            
Pro Glu His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Lys Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Val Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ala Leu Tyr Asp Gln 
                1285                1290                1295    
Asn Asp Asp Ala Ser Ile Glu Glu Ile Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Asn Phe Phe Gly 
        1315                1320                1325            
Glu Lys Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Cys Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 163
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #102

<400> 163
Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Asp Glu Asp Lys Arg 
            100                 105                 110         
Gly Glu Arg His Pro Ile Phe Gly Asn Ile Ala Ala Glu Val Lys Tyr 
        115                 120                 125             
His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Thr Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Lys Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Ala Leu Phe Lys Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Lys Thr Ile Glu Glu Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Leu Ser Ile Leu Thr Glu Lys Val Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Gly Leu Leu Gly Glu Val Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Ala Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Val Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Lys Lys Leu Lys 
                325                 330                 335     
Asp Phe Ile Lys Val Asn Ala Pro Asp Gln Tyr Asn Ala Ile Phe Lys 
            340                 345                 350         
Asp Lys Asn Lys Lys Gly Tyr Ala Gly Tyr Ile Glu Ser Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Leu Lys Ile Asn 
    370                 375                 380                 
Gly Ser Gly Asp Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Lys Ala Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Ile Leu Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Glu Ala Lys Gln Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Asp Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg 
                565                 570                 575     
Ile Val Asn Leu Thr Gly Leu Asp Lys Glu Phe Asn Ser Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Arg Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp 
        595                 600                 605             
Asp Lys Ala Asn Glu Gln Ile Ile Glu Asp Ile Ile Gln Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Gln Arg Leu Gln Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Ala Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Asn Lys Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Tyr 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Gln Ile Ile Gly Asp Val Asp Asp Ile 
705                 710                 715                 720 
Ala Asn Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Asn His Asn Pro Ala Asn Ile Ile Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asp Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Leu Leu 
    770                 775                 780                 
Gln Asp Ser Leu Lys Asn Leu Asp Asn Pro Val Asn Ile Lys Asn Pro 
785                 790                 795                 800 
Val Glu Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asn Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Ile Glu Val Val Arg Ala Arg Lys 
865                 870                 875                 880 
Ser Phe Trp Met Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Glu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu His Asp 
    930                 935                 940                 
Glu Asn Lys Lys Val Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Lys Lys Tyr Asn Val Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Lys Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Asp Ile Glu Thr Asn Lys Glu Asp Gly Glu Ile 
            1060                1065                1070        
Ala Trp Asn Lys Gln Lys Asp Phe Lys Ile Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ser Tyr Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Val Glu Lys Gly 
            1140                1145                1150        
Lys Ser Lys Lys Leu Lys Thr Val Gln Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Arg Asn Ile Arg Glu Asp Lys Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Leu Val Ile Pro Gln Arg Phe 
            1220                1225                1230        
Thr Thr Leu Leu Tyr His Ala His Arg Ile Asp Lys Ser Asn Gly Glu 
        1235                1240                1245            
Pro Glu His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Lys Asp 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Val Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ala Leu Phe Ala Gln 
                1285                1290                1295    
Asn Asp Asp Ala Ser Ile Glu Glu Ile Ala Thr Ser Phe Ile Ser Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Asn Phe Phe Gly 
        1315                1320                1325            
Glu Lys Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Cys Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 164
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #103

<400> 164
Met Lys Lys Ser Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Val Asn Lys Ser Glu Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Arg Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ser Ser Gln Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg 
                565                 570                 575     
Ile Asn Asp Leu Ile Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp 
        595                 600                 605             
Asp Lys Thr Asn Glu Glu Ile Ile Glu Asp Ile Ile Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Gln Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Gln Ile Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp Ile 
705                 710                 715                 720 
Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Asn Asp Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Arg Ser Lys Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Gln Asp Ser Leu Lys Glu Leu Gly Ser Asn Leu Asn Lys Lys Lys Pro 
785                 790                 795                 800 
Val Glu Asn Ser His Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp His 
            820                 825                 830         
Leu Ser Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Ile Glu Ile Val Arg Ala Arg Lys 
865                 870                 875                 880 
Ser Tyr Trp Val Lys Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Lys Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Lys Lys Tyr Asp Val Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser His Ser Glu Met Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Thr Glu Val Lys Tyr Ala Asp Gly Lys 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Asp Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asn Lys Gln Lys Asp Phe Glu Ile Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Val Tyr Trp Asp Thr Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Ser Ile 
        1155                1160                1165            
Met Glu Arg Ser Phe Phe Glu Glu Asn Pro Val Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr His Asn Val Gln Glu Asp Lys Leu Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Phe Glu Gly Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Thr Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Glu Leu Leu Tyr His Ala His Arg Ile Asp Asn Phe Asn Gly Ser 
        1235                1240                1245            
Thr Glu His Leu Glu Gln Lys Gln Lys Tyr Val Ser Glu His Lys Lys 
    1250                1255                1260                
Glu Phe Glu Glu Val Leu Ser Cys Val Glu Asn Phe Ala Asn Leu Tyr 
1265                1270                1275                1280
Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala Ala Ala Asp Ser 
                1285                1290                1295    
Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Asn Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe Asn Phe Leu Gly 
        1315                1320                1325            
Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys Glu Cys Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Glu 
                1365            

<210> 165
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #104

<400> 165
Met Glu Lys Ser Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Lys Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Glu Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Pro Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys Gln Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Val Asn Lys Leu Glu Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Ser Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ser Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Ala Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Asn Tyr His Ser Asp Glu Pro Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ser Phe Gln Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Ala Lys Leu Leu Ser Tyr Leu Asn Asn Glu Phe Glu Glu Phe Arg 
                565                 570                 575     
Ile Asn Asp Leu Ile Gly Leu Asp Lys Asp Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp 
        595                 600                 605             
Asp Lys Thr Asn Glu Gln Ile Ile Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Ser Gln Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Asn Asn Lys Thr Ile Leu Asp Phe Leu Ile Asp Asp Gly His 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe 
    690                 695                 700                 
Lys Thr Ile Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp Ile 
705                 710                 715                 720 
Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Asn Asp Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gly Tyr Gly Arg Asn Lys Ser Asn Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Gln Asp Ser Leu Lys Glu Phe Gly Ser Asp Leu Ser Lys Lys Lys Pro 
785                 790                 795                 800 
Val Glu Asn Ser His Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp His 
            820                 825                 830         
Leu Ser Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Ile Glu Ile Val Arg Asn Arg Lys 
865                 870                 875                 880 
Ser Tyr Trp Tyr Lys Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Lys Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Lys Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Lys Lys Tyr Asp Val Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser His Ser Glu Met Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Thr Glu Val Lys Tyr Ala Asp Gly Arg 
                1045                1050                1055    
Val Phe Glu Arg Pro Asp Ile Glu Thr Asn Lys Ala Asp Gly Glu Val 
            1060                1065                1070        
Val Trp Asn Lys Gln Lys Asp Phe Asp Ile Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Val Tyr Trp Asp Thr Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Ser Ile 
        1155                1160                1165            
Met Glu Arg Ser Phe Phe Glu Glu Asn Pro Val Ser Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr His Asn Val Gln Glu Asp Lys Leu Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Phe Glu Gly Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Thr Glu Leu Gln Lys Gly Asn Glu Val Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Glu Leu Leu Tyr His Ala His Arg Ile Asp Asn Phe Asn Gly Ser 
        1235                1240                1245            
Thr Glu His Leu Glu Gln Lys Gln Lys Tyr Val Ser Glu His Lys Lys 
    1250                1255                1260                
Glu Phe Glu Glu Val Leu Ser Cys Val Glu Asn Phe Ala Asn Leu Tyr 
1265                1270                1275                1280
Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala Ala Ala Glu Ser 
                1285                1290                1295    
Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Ala Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe Asn Phe Leu Gly 
        1315                1320                1325            
Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys Glu Cys Leu Ser 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Val Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Glu 
                1365            

<210> 166
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #105

<400> 166
Thr Lys Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Ala Asn Lys Ser Glu Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Arg Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Lys 
465                 470                 475                 480 
Val Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Asp Ser Gln Phe Phe Asp Ser Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu Tyr Arg 
                565                 570                 575     
Ile Lys Asp Leu Ile Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp 
        595                 600                 605             
Asp Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile Lys Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Gln Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Gln Ile Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp Ile 
705                 710                 715                 720 
Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Asn Asp Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu Lys Lys Leu 
    770                 775                 780                 
Gln Asn Ser Leu Lys Glu Leu Gly Ser Asn Leu Asn Glu Glu Lys Pro 
785                 790                 795                 800 
Val Glu Asn Ser His Leu Gln Asn Asp Gln Leu Phe Leu Tyr Tyr Ile 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Asp Glu Leu Asp Ile Asp His 
            820                 825                 830         
Leu Ser Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Leu Asp Ile Val Arg Ala Arg Lys 
865                 870                 875                 880 
Ala Glu Trp Val Arg Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Lys Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Lys Lys Tyr Asp Val Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser His Ser Glu Met Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Thr Val Val Arg Tyr Ala Asp Gly Lys 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Asp Thr Gly Glu Ile 
            1060                1065                1070        
Ala Trp Asn Lys Gln Thr Asp Phe Glu Lys Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Thr Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Val Tyr Trp Asp Thr Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Ser Ile 
        1155                1160                1165            
Met Glu Arg Ser Phe Phe Glu Glu Asn Pro Val Glu Phe Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr His Asn Val Gln Glu Asp Lys Leu Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Phe Glu Gly Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Thr Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Glu Leu Leu Tyr His Ala His Arg Ile Asp Asn Phe Asn Gly Ser 
        1235                1240                1245            
Thr Glu His Leu Glu Gln Lys Gln Lys Tyr Val Ser Glu His Lys Lys 
    1250                1255                1260                
Glu Phe Glu Glu Val Leu Ser Cys Val Glu Asn Phe Ala Asn Leu Tyr 
1265                1270                1275                1280
Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala Ala Ala Asp Ser 
                1285                1290                1295    
Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Asn Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe Asn Phe Leu Gly 
        1315                1320                1325            
Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys Glu Cys Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Glu 
                1365            

<210> 167
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #106

<400> 167
Thr Lys Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Ile Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys Tyr Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Ala Asn Lys Ser Glu Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Asn Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Arg Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Lys 
465                 470                 475                 480 
Val Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Tyr Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Asp Ser Gln Phe Phe Asp Ser Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu Tyr Arg 
                565                 570                 575     
Ile Lys Asp Leu Ile Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ala Phe Leu Asp 
        595                 600                 605             
Asp Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile Lys Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Lys Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Ala Asn Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Pro Phe 
    690                 695                 700                 
Lys Gln Ile Ile Gln Lys Ser Gln Val Val Gly Asp Val Asp Asp Ile 
705                 710                 715                 720 
Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Asn Asp Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu Lys Lys Leu 
    770                 775                 780                 
Gln Asn Ser Leu Lys Glu Leu Gly Ser Asn Leu Asn Glu Glu Lys Pro 
785                 790                 795                 800 
Val Glu Asn Ser His Leu Gln Asn Asp Gln Leu Phe Leu Tyr Tyr Ile 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Asp Glu Leu Asp Ile Asp His 
            820                 825                 830         
Leu Ser Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Leu Asp Ile Val Arg Ala Arg Lys 
865                 870                 875                 880 
Ala Glu Trp Val Arg Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Asp Phe Glu Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Ser Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Lys Lys Tyr Asp Val Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser His Ser Glu Met Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Thr Val Val Arg Tyr Ala Asp Gly Lys 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Asp Thr Gly Glu Ile 
            1060                1065                1070        
Ala Trp Asn Lys Gln Thr Asp Phe Glu Lys Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Thr Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Val Tyr Trp Asp Thr Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Phe Val Val Ala Asp Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Ser Ile 
        1155                1160                1165            
Met Glu Arg Ser Phe Phe Glu Glu Asn Pro Val Glu Phe Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr His Asn Ile Arg Glu Asp Lys Leu Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Phe Glu Gly Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Ser Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Gly His Leu 
            1220                1225                1230        
Val Glu Leu Leu Tyr His Ala His Arg Ile Asp Asn Phe Asn Gly Ser 
        1235                1240                1245            
Thr Glu Tyr Leu Glu Gln Lys Gln Asn Tyr Val Ser Glu His Lys Lys 
    1250                1255                1260                
Glu Phe Glu Lys Val Leu Ser Cys Val Glu Asp Phe Ala Asn Leu Tyr 
1265                1270                1275                1280
Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala Val Ala Asp Ser 
                1285                1290                1295    
Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Asn Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe Asn Phe Leu Gly 
        1315                1320                1325            
Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys Glu Cys Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Glu 
                1365            

<210> 168
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #107

<400> 168
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Val Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Leu Leu Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Phe Ile Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Ser Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Gln Asn Leu Pro Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Lys Thr Tyr Gln Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Leu Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Leu Asp Tyr Leu Glu Lys Glu Phe Asp Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Leu Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu 
705                 710                 715                 720 
Lys Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Asp Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Ala Arg Phe Asn Thr Glu Leu Asp 
    930                 935                 940                 
Glu Asn Asn Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Ala Leu Leu Lys Lys Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Pro Lys Tyr Asn Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Lys Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Val Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Ile Gln Glu Asp Asn Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile His Lys Val Asn Gly Glu 
        1235                1240                1245            
Pro Glu His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Lys Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Asn Asn Leu Glu Lys Ile Lys Glu Leu Tyr Asp Gln 
                1285                1290                1295    
Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Ile Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 169
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #108

<400> 169
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Glu Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Val Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Leu Leu Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Phe Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asp Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Gln Asn Leu Pro Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Ala Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Asn 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Arg Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Lys Thr Tyr Gln Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Gly Leu Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Leu Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asp Ser Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Leu Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu 
705                 710                 715                 720 
Lys Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Glu Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Asp Ala Ile Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Asp Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Leu Asp 
    930                 935                 940                 
Glu Asn Asn Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Lys Ala Leu Leu Lys Lys Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Pro Lys Tyr Asn Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Lys Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Val Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys His Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Arg 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Ile Gln Glu Asp Asn Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile His Lys Val Asn Gly Glu 
        1235                1240                1245            
Pro Glu His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Lys Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu Leu Tyr Asp Gln 
                1285                1290                1295    
Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Ile Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 170
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #109

<400> 170
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Val Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Ser His Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Gly Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Thr Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Val Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Leu Leu Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Phe Ile Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Glu Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Glu Leu Glu Val Leu Leu Ala Gln Ile Gly Asp Asn Tyr Ala Glu 
        275                 280                 285             
Leu Phe Leu Ser Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Val Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asn Glu His Gln Met Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Gln Lys Leu Ser Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Val Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
        355                 360                 365             
Gln Glu Ala Phe Tyr Lys Tyr Leu Lys Gly Leu Leu Asn Lys Ile Glu 
    370                 375                 380                 
Gly Ser Gly Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Arg Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro Phe 
            420                 425                 430         
Leu Ala Asp Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Ala Asp Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Ile Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asn Gln Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Lys Thr Glu Gln Gly Lys Thr Ala Gln Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr 
545                 550                 555                 560 
Lys Asp Lys Leu Met Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Tyr Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Arg Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Ser Lys Asn Glu Lys Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Asn Tyr Ser 
625                 630                 635                 640 
Asp Leu Leu Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Glu Leu Ile His Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Asn 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu 
705                 710                 715                 720 
Asn Gln Val Val Ser Asp Ile Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Ile Met Gly 
            740                 745                 750         
Gly His Gln Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Phe Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Gly Leu 
    770                 775                 780                 
Thr Asp Ser Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Ser Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Tyr 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Lys Asp Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Tyr Trp Ser Lys Leu Leu Ser Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Thr Asp 
    930                 935                 940                 
Glu Asn Asn Lys Lys Ile Arg Gln Val Lys Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Ile Gly Lys Ala Leu Leu Gly Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Pro His Phe His Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Gly His Lys Glu Asn Lys Ala Thr Ala Lys Lys Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Lys Asp Asp Val Arg Thr Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Val Asn Lys Lys Asn Gly Glu Ile 
            1060                1065                1070        
Ile Trp Lys Lys Asp Glu His Ile Ser Asn Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Phe Tyr Trp Asp Thr Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Ile Val Ala Tyr Ser Ile Leu Val Ile Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ser Lys Lys Leu Lys Thr Val Lys Asp Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Thr Thr Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Arg 
    1170                1175                1180                
Lys Gly Tyr Arg Asn Val Gln Glu Glu Asn Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Arg Glu Leu Gln Lys Gly Asn Glu Ile Val Leu Pro Asn His Leu 
            1220                1225                1230        
Gly Thr Leu Leu Tyr His Ala Lys Asn Ile His Lys Val Asp Gly Glu 
        1235                1240                1245            
Pro Lys His Leu Glu Gln Lys Gln Asp Tyr Val Glu Lys His Lys Asp 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asp Val Val Ser Asn Phe Ser Lys Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Glu Ser Asn Leu Glu Lys Ile Lys Glu Leu Tyr Ala Gln 
                1285                1290                1295    
Asn Asn Asn Glu Asp Ile Lys Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Ile Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Ile Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Gly Asp 
                1365            

<210> 171
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #110

<400> 171
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Lys Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Leu Asp Val 
                165                 170                 175     
Arg Asn Thr Asp Val Gln Lys Leu Phe Gln Glu Phe Leu Glu Val Tyr 
            180                 185                 190         
Asp Thr Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Lys Leu Glu Arg 
    210                 215                 220                 
Leu Leu Ala Gln Phe Pro Asn Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Phe Leu Ala Leu Ile Val Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Val Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asp Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Ala Asn Leu Pro Asp Lys Tyr Lys Glu Val Phe Ser 
            340                 345                 350         
Asp Ala Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Asn 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His Leu 
                405                 410                 415     
Ala Glu Met Lys Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Asn Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Glu Thr Tyr Gln Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Gly Leu Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Leu Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Ile Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asp Ser Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Lys Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Leu Phe Thr Lys Lys Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Arg 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Asp Ser Asp Asn Leu 
705                 710                 715                 720 
Lys Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly Tyr Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Ile Lys Asn Leu Gly Ser Asp Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Asp Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Leu Asp 
    930                 935                 940                 
Glu Asn Asn Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Lys Ala Leu Leu Thr Lys Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Pro Lys Tyr Asn Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Lys Ser Ala Thr Glu Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Lys Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Val Asn Asn Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys His Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Leu Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Arg Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Arg 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Ile Gln Glu Asp Lys Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Lys Leu Leu Tyr His Ala Lys Arg Ile Asn Lys Val Asn Gly Glu 
        1235                1240                1245            
Pro Glu His Ile Glu Gln Lys Gln Glu Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Lys Asp Leu Leu Asn Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu Leu Tyr Glu Gln 
                1285                1290                1295    
Asn Glu Asp Asp Ser Val Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Val Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 172
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #111

<400> 172
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Ile Ala Asp Glu Val Lys Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Asp Asp Pro Leu Asp Val 
                165                 170                 175     
Arg Asn Thr Asp Ser Gln Lys Leu Phe Gln Glu Phe Leu Glu Ala Tyr 
            180                 185                 190         
Asp Ser Thr Gln Glu Asp Ser His Leu Ser Glu Gln Lys Ile Asp Ile 
        195                 200                 205             
Ser Asp Ile Ile Thr Glu Lys Ile Ser Lys Ser Arg Lys Val Glu Ala 
    210                 215                 220                 
Leu Leu Ala Gln Phe Pro Asn Gln Lys Lys Asn Ser Phe Phe Gly Asn 
225                 230                 235                 240 
Leu Val Ser Leu Val Phe Gly Leu Met Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Val Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asn Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asp Glu His Asn Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Asp Phe Ile Arg Ala Asn Leu Pro Asp Val Phe Lys Glu Val Phe Ser 
            340                 345                 350         
Asp Ala Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Gly Lys Val Asp 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Ile Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His Leu 
                405                 410                 415     
Ala Glu Met Lys Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Gln Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Ala Ser Phe Asn Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Ile Asp Lys Asn Ser Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Asn Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Thr Gly Glu Ala Arg Glu Phe Phe Asp Ala Asn Leu 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Gly Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Leu Asp Phe Leu Glu Lys Asn Phe Asp Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Ile Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Tyr Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Glu Phe Met Asp 
        595                 600                 605             
Asp Ser Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Lys Tyr Ala 
625                 630                 635                 640 
Asp Leu Phe Asp Lys Lys Val Ile Asp Lys Leu Ala Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Arg 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Asp Glu Ile Ala Lys Ala Gln Val Ile Gly Asp Ser Asp Asp Leu 
705                 710                 715                 720 
His Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly Tyr Glu Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ala Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Ile Lys Asn Leu Gly Ser Asp Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Asp Val Val Lys Arg Met Lys 
865                 870                 875                 880 
Ser Phe Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Arg Glu Phe Asp 
    930                 935                 940                 
Glu Asn Asn Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Lys Ala Leu Leu Thr Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Pro Lys Tyr Asn Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Lys Ser Ala Thr Glu Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Met Phe Lys Thr Lys Val Lys Leu Ala Asp Gly Arg 
                1045                1050                1055    
Val Ile Glu Arg Pro Met Ile Glu Val Asn Glu Glu Thr Gly Glu Ile 
            1060                1065                1070        
Ala Trp Asp Lys Thr Lys His Phe Ala Thr Val Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ala Arg Lys Asn Tyr Leu Asp Thr Gln Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Ile Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Arg Phe Glu Asn Asn Ser Val Ala Phe Leu Glu Arg 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Ile Gln Glu Asn Lys Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Lys Leu Leu Tyr His Ala Lys His Ile Asn Ser Val Asn Gly Glu 
        1235                1240                1245            
Pro Glu His Ile Glu Gln Lys Gln Asn Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Lys Asp Leu Leu Asn Tyr Ile Arg Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Asn Glu Leu Phe Glu Glu 
                1285                1290                1295    
Asn Glu Glu Asp Ser Val Ser Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Val Leu Ser 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 173
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #112

<400> 173
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Met 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         
Gly Glu Arg His Pro Ile Phe Gly Asn Ile Ala Asp Glu Val Lys Tyr 
        115                 120                 125             
His Glu Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Asp Glu Pro Ile Asp Ile 
                165                 170                 175     
Arg Asn Thr Asp Ser Gln Lys Leu Phe Gln Glu Phe Leu Asp Ala Tyr 
            180                 185                 190         
Asp Ser Ile Gln Glu Asp Ser His Leu Ser Glu Glu Lys Thr Asp Ile 
        195                 200                 205             
Ser Asp Ile Ile Thr Ala Lys Ile Ser Lys Ser Arg Lys Val Glu Ala 
    210                 215                 220                 
Leu Leu Ala Gln Phe Pro Asn Gln Lys Lys Asn Ser Phe Phe Gly Asn 
225                 230                 235                 240 
Leu Val Ser Leu Val Phe Gly Leu Met Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Met Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Val Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Thr Asn Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Asp Glu His Asn Lys Asp Lys Gln Leu Leu Lys 
                325                 330                 335     
Asp Phe Ile Arg Ala Asn Ile Gly Asp Val Phe Lys Glu Val Phe Tyr 
            340                 345                 350         
Asp Thr Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Gly Lys Val Asp 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Ile Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His Leu 
                405                 410                 415     
Ala Glu Met Lys Ala Ile Leu Arg Arg Gln Gly Glu Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Gln Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Ser Phe Asn Ser Asn Glu Lys Ile Thr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Val Ile Asp Lys Ser Ser Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Asn Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Thr Gly Glu Ala Arg Glu Phe Phe Asp Ala Asn Leu 
    530                 535                 540                 
Lys Lys Glu Ile Phe Asp Gly Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Leu Asp Phe Leu Glu Lys Asn Phe Asp Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Ile Thr Gly Leu Asp Asn Glu Phe Asn Ala Ser Tyr Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Glu Phe Met Asp 
        595                 600                 605             
Asp Ser Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Leu Lys Tyr Glu 
625                 630                 635                 640 
Asp Leu Phe Asp Lys Lys Val Ile Asp Lys Leu Ser Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Lys Asp 
            660                 665                 670         
Lys Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly His 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Asp Glu Ile Ala Lys Ala Gln Val Ile Gly Asp Gly Asp Asp Leu 
705                 710                 715                 720 
His Gln Val Val Gln Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly Tyr Asp Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ala Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Gly Arg Leu 
    770                 775                 780                 
Lys Glu Ala Ile Gln Asp Leu Gly Ser Asp Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Asn Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Leu Asp Asn Arg Val Leu Val Ser Ser Ala Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Asn Asp Val Val Lys Arg Met Lys 
865                 870                 875                 880 
Ser Phe Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Ala Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Arg Glu Phe Asp 
    930                 935                 940                 
Glu Asn Asn Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Lys Ala Leu Leu Thr Arg Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Pro Lys Tyr Asn Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Lys Ser Ala Thr Glu Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Met Phe Lys Thr Thr Ile Lys Leu Ala Asp Gly Arg 
                1045                1050                1055    
Val Val Glu Arg Pro Met Ile Glu Ala Asn Glu Glu Thr Gly Glu Ile 
            1060                1065                1070        
Ala Trp Asp Lys Thr Lys His Phe Ala Thr Val Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ala Arg Lys Asn Tyr Leu Asn Thr Gln Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Phe Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ser Lys Lys Leu Lys Thr Val Lys Glu Met Ile Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Arg Phe Glu Ser Asn Ser Val Ala Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Ile Arg Glu Asn Thr Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Gln His Leu 
            1220                1225                1230        
Val Lys Leu Leu Tyr His Ala Lys His Ile Asn Ser Leu Asn Gly Lys 
        1235                1240                1245            
Pro Glu His Ile Glu Lys Lys Arg Asn Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Thr Asp Leu Leu His His Ile Lys Asp Phe Ser Glu Lys Asn 
1265                1270                1275                1280
Ile Leu Ala Ala Lys Asn Leu Glu Lys Ile Asn Glu Ala Phe Glu Glu 
                1285                1290                1295    
Asn Glu Glu Asp Ser Val Ser Glu Leu Ala Gln Ser Phe Ile Ser Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Val Leu Ser 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Glu 
                1365            

<210> 174
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #113

<400> 174
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Arg Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys His Pro Ile Phe Gly Asn Leu Ala Glu Glu Lys Ala Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Asn Asp Val Gln Lys Leu Phe Asn Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Lys Thr Phe Glu Gly Ser His Leu Ser Glu Gln Asn Ala Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Ile Leu Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Phe Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Ser Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Gln Asn Leu Pro Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Met Arg Asp Tyr Gln Phe Leu Asp Ser Gly Gln 
    530                 535                 540                 
Lys Lys Glu Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val Thr 
545                 550                 555                 560 
Glu Lys Asp Ile Ile Asp Tyr Leu Glu Asn Glu Ile Asp Glu Tyr Asp 
                565                 570                 575     
Gly Val Glu Leu Lys Gly Leu Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Glu Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Thr 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asp Leu 
705                 710                 715                 720 
Lys Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Tyr Lys Arg Leu 
    770                 775                 780                 
Glu Asp Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Gln 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Arg Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Glu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Ala Arg Phe Asn Thr Glu Leu Asp 
    930                 935                 940                 
Glu Asn Asn Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Ala Leu Leu Lys Lys Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Pro Lys Tyr Asn Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Lys Lys Ala Thr Glu Lys Val Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Lys Glu Val Lys Tyr Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Val Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Leu Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Ile Ala Tyr Ser Val Leu Val Ile Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Thr Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Lys Asn Ile Gln Glu Asp Asn Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile His Lys Ile Asn Gly Glu 
        1235                1240                1245            
Pro Ile His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Lys Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asn Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Asn Asn Leu Glu Lys Ile Lys Glu Leu Tyr Asp Gln 
                1285                1290                1295    
Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Leu Gly 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Ile Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 175
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #114

<400> 175
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Arg Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Thr Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys Tyr Pro Ile Phe Gly Asn Leu Ala Glu Glu Lys Ala Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Phe Asn Ser 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Asp Phe Val Glu Ile Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Gly Ser His Leu Ser Glu Gln Asn Ala Gln Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 
Ile Leu Lys Leu Phe Pro Asn Glu Lys Asn Asn Gly Leu Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     
Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Ser Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Met Arg Asp Tyr Gln Phe Leu Asp Ser Gly Gln 
    530                 535                 540                 
Lys Lys Glu Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val Thr 
545                 550                 555                 560 
Glu Lys Asp Ile Ile Asp Tyr Leu His Asn Glu Val Asp Gly Tyr Asp 
                565                 570                 575     
Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Leu Asp 
        595                 600                 605             
Asp Ala Glu Asn Glu Ala Ile Leu Glu Asp Ile Val His Thr Leu Thr 
    610                 615                 620                 
Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Lys Tyr Asp 
625                 630                 635                 640 
Asn Leu Phe Asp Glu Lys Val Leu Lys Lys Leu Thr Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Thr Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Thr 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asp Val 
705                 710                 715                 720 
Lys Gln Val Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Gln Gly Lys Arg Asn Ser Gln Gln Arg Tyr Lys Arg Leu 
    770                 775                 780                 
Glu Asp Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu Asn Pro 
785                 790                 795                 800 
Thr Asp Asn Asn Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Gln 
            820                 825                 830         
Leu Ser Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Asp Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Arg Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Glu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Ala Arg Phe Asn Thr Glu Leu Asp 
    930                 935                 940                 
Glu Asn Asp Lys Lys Ile Arg Thr Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Pro Lys Tyr Asn Ser Lys Arg Tyr Ile Ser Arg 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Lys Lys Ala Thr Glu Lys Val Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Lys Glu Val Ser Tyr Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Val Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asn Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Leu Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Ile Ala Tyr Ser Val Leu Val Ile Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Thr Ala Phe Glu Lys Asn Pro Ile Thr Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Lys Asn Ile Gln Glu Glu Asn Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Ile Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile His Lys Ile Asn Gly Glu 
        1235                1240                1245            
Pro Ile His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Lys Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asn Tyr Ile Ser Glu Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Asn Asn Leu Glu Lys Ile Lys Ser Leu Tyr Asp Gln 
                1285                1290                1295    
Asn Glu Asp Ala Asp Ile Asp Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Leu Gly 
        1315                1320                1325            
Val Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Ser Glu Ile Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 176
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #115

<400> 176
Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Ile Thr Ala Glu Gly Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala Thr Leu Asp Asp Ala 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Pro Asp Asp Lys Arg 
            100                 105                 110         
Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val Glu Glu Lys Ala Tyr 
        115                 120                 125             
His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 
Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Phe Asn Ser 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp Phe Leu Asp Thr Tyr 
            180                 185                 190         
Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu Asn Ser Lys Gln Leu 
        195                 200                 205             
Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp Arg 
    210                 215                 220                 
Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser Gly Ile Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys Tyr Phe 
                245                 250                 255     
Asn Leu Asp Glu Lys Ala Pro Leu His Phe Ser Lys Glu Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly Asp Asp Tyr Ser Asp 
        275                 280                 285             
Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Gly Thr Glu Ala Pro Leu Ser Ser Ala 
305                 310                 315                 320 
Met Ile Met Arg Tyr Lys Glu His Glu Glu Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Ala Tyr Ile Arg Asn Ile Ser Leu Glu Thr Tyr Asn Glu Val Phe Lys 
            340                 345                 350         
Asp Asn Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Val Tyr Leu Lys Asn Leu Leu Ser Asn Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala Lys Phe Tyr Pro Phe 
            420                 425                 430         
Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro Trp Asn Phe Glu Asp 
465                 470                 475                 480 
Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Phe Ile Ala Glu Ser Met Arg Asp Tyr Gln Phe Leu Asp Ser Lys Gln 
    530                 535                 540                 
Lys Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp Lys Arg Lys Val Thr 
545                 550                 555                 560 
Asp Lys Asp Ile Ile Glu Tyr Leu His Ala Glu Ile Asp Gly Tyr Asp 
                565                 570                 575     
Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ser Ser Leu Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys Glu Phe Leu Asp 
        595                 600                 605             
Asp Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile His Thr Leu Thr 
    610                 615                 620                 
Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ser Lys Phe Glu 
625                 630                 635                 640 
Asn Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Glu Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ile 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Lys Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp Lys Asp Asn Ile 
705                 710                 715                 720 
Lys Gln Val Val Gln Ala Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly Arg Lys Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Tyr Thr Asn Gln Gly Lys Ser Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ser Leu Lys Glu Leu Gly Ser Lys Ile Leu Lys Glu Asn Ile 
785                 790                 795                 800 
Ile Asp Asn Asn Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Arg 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Leu Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Lys Val Leu Val Ser Ser Ala Ser Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Val Val Lys Lys Arg Lys 
865                 870                 875                 880 
Thr Leu Trp Tyr Gln Leu Leu Lys Ser Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Arg Glu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Leu Leu Asp Glu Arg Phe Asn Asn Lys Lys Asp 
    930                 935                 940                 
Glu Asn Asn Lys Thr Val Arg Thr Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ser Leu Val Ser Gln Phe Arg Lys Asp Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Phe His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Ser Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Pro Lys Tyr Asn Ser Lys Arg Tyr Ile Ser Lys 
    1010                1015                1020                
Ser Phe Arg Glu Arg Lys Ser Ala Thr Glu Lys Val Tyr Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Ile Phe Lys Lys Ser Ile Ser Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Leu Ile Glu Val Asn Glu Glu Thr Gly Glu Ser 
            1060                1065                1070        
Val Trp Asn Lys Glu Ser Asp Leu Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Asn His Gly 
    1090                1095                1100                
Leu Asp Arg Lys Gly Leu Phe Asn Ala Asn Leu Ser Ser Lys Pro Lys 
1105                1110                1115                1120
Pro Asn Ser Glu Tyr Leu Asp Pro Lys Lys Tyr Gly Gly Tyr Ala Gly 
                1125                1130                1135    
Ile Ser Asn Ser Phe Ala Val Leu Val Lys Gly Thr Ile Glu Lys Gly 
            1140                1145                1150        
Ala Lys Lys Lys Ile Thr Asn Val Leu Glu Phe Gln Gly Ile Ser Ile 
        1155                1160                1165            
Leu Asp Arg Ile Thr Tyr Arg Lys Asp Lys Leu Asn Phe Leu Leu Glu 
    1170                1175                1180                
Lys Gly Tyr Lys Asp Ile Glu Glu Glu Leu Ile Ile Glu Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Ser Asp Gly Ser Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ile Gly Glu Ile His Lys Gly Asn Gln Ile Phe Leu Ser Gln Lys Phe 
            1220                1225                1230        
Val Lys Leu Leu Tyr His Ala Lys Arg Ile Ser Asn Thr Ile Gly Asn 
        1235                1240                1245            
Glu Asn His Arg Glu Gln Lys Gln Lys Tyr Val Glu Asn His Lys Lys 
    1250                1255                1260                
Glu Phe Glu Glu Leu Phe Tyr Tyr Ile Leu Glu Phe Asn Glu Asn Tyr 
1265                1270                1275                1280
Val Gly Ala Lys Lys Asn Gly Glu Leu Leu Lys Ser Ala Phe Gln Ser 
                1285                1290                1295    
Trp Gln Asn His Ser Ile Asp Glu Leu Cys Ser Ser Phe Ile Gly Leu 
            1300                1305                1310        
Phe Glu Leu Thr Ser Arg Gly Ser Ala Ala Asp Phe Glu Phe Leu Gly 
        1315                1320                1325            
Val Lys Ile Pro Arg Tyr Arg Tyr Thr Pro Ser Ser Leu Leu Lys Asp 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Val Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ala Lys Leu Gly Glu Asp 
                1365            

<210> 177
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #116

<400> 177
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Arg Phe Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Ser Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ala Phe Asp Ile 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln Val 
        195                 200                 205             
Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     
Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Asp Glu Val Phe Ser 
            340                 345                 350         
Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Asn Ser Asp Glu Ala Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Ile Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly Gln 
    530                 535                 540                 
Lys Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val Thr 
545                 550                 555                 560 
Glu Lys Asp Ile Ile His Tyr Leu His Asn Glu Val Asp Gly Tyr Asp 
                565                 570                 575     
Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp 
        595                 600                 605             
Asp Ala Lys Asn Glu Ala Ile Leu Glu Asn Ile Val His Thr Leu Thr 
    610                 615                 620                 
Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp 
625                 630                 635                 640 
Ser Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Thr Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Asn 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe 
    690                 695                 700                 
Lys Glu Ile Ile Gln Lys Ala Gln Val Ile Gly Lys Thr Asp Asp Val 
705                 710                 715                 720 
Lys Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile 
    770                 775                 780                 
Glu Asp Ser Leu Lys Asn Leu Ala Ser Gly Ile Leu Lys Glu Asn Pro 
785                 790                 795                 800 
Thr Asp Asn Asn Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Ala Leu Asp Ile Asn Gln 
            820                 825                 830         
Leu Ser Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys Asp Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Gln Lys Arg Lys 
865                 870                 875                 880 
Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Glu Arg Lys 
                885                 890                 895     
Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp Glu Arg Asp 
            900                 905                 910         
Lys Val Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Val Asn 
    930                 935                 940                 
Glu Lys Asp Lys Lys Asn Arg Thr Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Arg Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Lys Ala Ile Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Gln Lys Tyr Asp Leu Lys Arg Tyr Ile Ser Arg 
    1010                1015                1020                
Ser Pro Lys Glu Val Glu Lys Ala Thr Glu Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Leu Asn Phe Phe Lys Glu Glu Val His Tyr Ala Asp Gly Thr 
                1045                1050                1055    
Ile Val Lys Arg Glu Asn Ile Glu Tyr Ser Lys Asp Thr Gly Glu Ile 
            1060                1065                1070        
Ala Trp Asn Lys Glu Lys Asp Phe Ala Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Leu Pro Gln Val Asn Ile Val Lys Lys Thr Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Ser Leu Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Ile Ala Tyr Ser Val Leu Val Ile Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Lys Ala Ala Phe Glu Glu Asn Pro Ile Thr Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Lys Asn Ile Arg Lys Glu Asn Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Ile Val Leu Pro Val His Leu 
            1220                1225                1230        
Thr Thr Leu Leu Tyr His Ala Lys Arg Ile His Lys Ile Asn Gly Glu 
        1235                1240                1245            
Pro Ile His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asn Leu Ile Ser Glu Phe Ser Gln Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Asn Asn Leu Glu Lys Ile Lys Ser Leu Tyr Asp Gln 
                1285                1290                1295    
Asn Glu Asp Ala Asp Ile Asp Gln Leu Ala Asn Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Leu Gly 
        1315                1320                1325            
Val Asp Ile Asp Arg Lys Arg Tyr Thr Ser Val Ser Glu Ile Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 178
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - marginal ancestral sequence reconstruction method–
      node #117

<400> 178
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Ser Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu Tyr 
        115                 120                 125             
His Lys Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp Ile 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln Val 
        195                 200                 205             
Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 
Val Leu Lys Leu Phe Ser Asp Glu Lys Ser Thr Gly Leu Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     
Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala Asp 
        275                 280                 285             
Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Asp Glu Val Phe Ser 
            340                 345                 350         
Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Ile Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly Gln 
    530                 535                 540                 
Lys Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val Thr 
545                 550                 555                 560 
Glu Lys Asp Ile Ile His Tyr Leu His Asn Glu Val Asp Gly Tyr Asp 
                565                 570                 575     
Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp 
        595                 600                 605             
Asp Ala Lys Asn Glu Ala Ile Leu Glu Asn Ile Ile His Thr Leu Thr 
    610                 615                 620                 
Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp 
625                 630                 635                 640 
Ser Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Thr Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Asp 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe 
    690                 695                 700                 
Lys Glu Ile Ile Gln Lys Ala Gln Val Val Gly Lys Thr Asp Asp Val 
705                 710                 715                 720 
Lys Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile 
    770                 775                 780                 
Glu Asp Ala Leu Lys Asn Leu Ala Pro Gly Ile Leu Lys Glu Asn Pro 
785                 790                 795                 800 
Thr Asp Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Lys Ala Leu Asp Ile Asn Gln 
            820                 825                 830         
Leu Ser Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys Asp Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Gln Lys Arg Lys 
865                 870                 875                 880 
Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Glu Arg Lys 
                885                 890                 895     
Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp Glu Arg Asp 
            900                 905                 910         
Lys Val Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Val Asn 
    930                 935                 940                 
Glu Lys Asp Lys Lys Asn Arg Thr Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Lys Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Lys Ala Ile Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Gln Lys Tyr Asp Leu Lys Arg Tyr Ile Ser Arg 
    1010                1015                1020                
Ser Pro Lys Asp Val Glu Lys Ala Thr Glu Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Leu Asn Phe Phe Lys Glu Glu Val His Tyr Ala Asp Gly Thr 
                1045                1050                1055    
Ile Val Lys Arg Glu Asn Ile Glu Tyr Ser Asn Asp Thr Gly Glu Ile 
            1060                1065                1070        
Ala Trp Asn Lys Glu Lys Asp Phe Ala Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Leu Pro Gln Val Asn Ile Val Lys Lys Thr Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Leu Phe Asp Asn Asn Ile Val Ser Lys Lys Asp Ala Ser Lys Leu Ile 
1105                1110                1115                1120
Pro Ile Lys Ser Ser Leu Ser Pro Glu Lys Tyr Gly Gly Tyr Ala Arg 
                1125                1130                1135    
Pro Thr Ile Ala Tyr Ser Val Leu Val Ile Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Arg Ile Lys Glu Met Val Gly Ile Thr Ile 
        1155                1160                1165            
Gln Asp Lys Lys Lys Phe Glu Ala Asn Pro Ile Thr Tyr Leu Glu Glu 
    1170                1175                1180                
Arg Gly Tyr Lys Asn Ile Asn Ser Asn Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Phe Asn Asp Gly Gln Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ser Ile Glu Leu Gln Lys Gly Asn Glu Leu Ile Leu Pro Tyr His Leu 
            1220                1225                1230        
Thr Ala Leu Leu Tyr His Ala Gln Arg Ile Asn Lys Ile Asn Gly Glu 
        1235                1240                1245            
Pro Ile His Lys Glu Gln Lys Gln Gln Tyr Val Glu Thr His Gln Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Thr Thr Ile Ile Ser Leu Ser Lys Lys Tyr 
1265                1270                1275                1280
Ile Gln Lys Pro Asn Val Glu Ser Leu Leu Gln Gln Ala Phe Asp Gln 
                1285                1290                1295    
Asn Ala Asp Lys Asp Ile Tyr Gln Leu Ser Glu Ser Phe Ile Ser Leu 
            1300                1305                1310        
Leu Lys Leu Thr Ser Phe Gly Ala Pro Gly Ala Phe Lys Phe Leu Gly 
        1315                1320                1325            
Val Glu Ile Ser Gln Ser Arg Tyr Lys Ser Val Ser Gln Phe Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 179
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #60

<400> 179
Met Lys Lys Glu Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Lys Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Leu Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Ser Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
His Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Gln Glu Glu Ile Ser Lys Val Asp Pro Ser 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Asp Asp Arg His Pro Leu Phe Gly Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Met Glu 
    130                 135                 140                 
Asn Asp Glu Lys His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Ile Glu Gly Asp Leu Ser Ser 
                165                 170                 175     
Lys Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Leu Val Gln Val Phe 
            180                 185                 190         
Asn Asp Ala Leu Glu Glu Glu His Leu Ser Glu Glu Ala Ile Asp Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Arg Ser Ala Arg Ala Lys Glu 
    210                 215                 220                 
Val Val Lys Leu Phe Ala Ser Glu Lys Lys Gln Ala Cys Ile Ser Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Val Gly Leu Lys Gly Asn Leu Lys Lys Val Phe 
                245                 250                 255     
Gly Asp Ser Glu Asp Thr Ala Ile His Phe Ser Lys Asp Asn Tyr Glu 
            260                 265                 270         
Glu Asp Val Glu Ala Ile Arg Asp Thr Ile Pro Asp Glu Tyr Ser Glu 
        275                 280                 285             
Leu Phe Glu Ala Ile Lys Ala Leu Tyr Asp Ala Ile Val Leu Ser Gly 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Asn Asn Thr Glu Ala Lys Val Ser Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Gln His Arg Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Asn Phe Val Lys Thr Tyr Leu Pro Glu Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Asp Thr Lys Asn Gly Tyr Ser Ala Tyr Ile Lys Gly Gly Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Asn Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Thr Ser Asp Asn Gly Val Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Ser Ile Leu Ala Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Gly Asn Gly Arg Phe Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Glu Asp Gly Val Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Asn Cys Thr Tyr Leu Pro Gly Glu Thr Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Leu Asn Glu Leu Asn Asn Val Arg 
        515                 520                 525             
Leu Thr Ile Asp Lys Gly Lys Trp Arg Glu Arg Phe Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Arg Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Glu Asp Tyr Leu Lys Arg Glu Tyr Gly Glu Phe Asp 
                565                 570                 575     
Ser Ala Asp Ile Ser Gly Ile Asp Gly Glu Phe Lys Ser Ser Leu Gly 
            580                 585                 590         
Ser Tyr His Asp Phe Cys Lys Ile Leu Val Lys Ala Asp Ser Leu Asp 
        595                 600                 605             
Glu Glu Asp Tyr Lys Asp Ile Ile Glu Glu Ile Ile Lys Trp Lys Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Leu Lys Arg Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Glu Ile Leu Thr Glu Glu Gln Ile Lys Lys Ile Cys Lys Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Val Arg Asp 
            660                 665                 670         
Lys Glu Thr Gly Lys Ser Ile Ile Asp Val Leu Arg Glu Thr Asp Pro 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Leu Ser Asp Glu Asp Leu Gly Phe 
    690                 695                 700                 
Gln Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Glu Asn Leu 
705                 710                 715                 720 
Lys Glu Ile Val Glu Asp Leu Pro Gly Ser Pro Ala Val Lys Arg Gly 
                725                 730                 735     
Ile Asn Gln Ala Leu Arg Ile Val Asp Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Gly His Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Glu Lys Gln 
        755                 760                 765             
Gln Ser Lys Arg Gly Arg Arg Ser Thr Lys Lys Arg Leu Lys Arg Leu 
    770                 775                 780                 
Gln Glu Ala Leu Lys Asn Leu Gly Thr Asn Leu Trp Lys Glu Leu Leu 
785                 790                 795                 800 
Arg Asp Asp Asn Glu Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Met Gly Lys Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Asp Asp 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Val Ser Arg Glu Glu Asn Ala 
    850                 855                 860                 
Arg Lys Thr Asp Asn Leu Pro Ser Pro Glu Ile Arg Arg Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Met Leu Leu Asn Ala Gly Leu Ile Ser Lys Lys Lys 
                885                 890                 895     
Phe Asp Arg Leu Thr Arg Thr Asp Arg Gly Glu Phe Thr Glu Asp Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Thr Leu Leu Lys Gln Arg Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Lys Asn Thr Lys Ile Val Ser Val Lys Ala 
945                 950                 955                 960 
Asn Leu Val Ser Glu Phe Arg Gln Asp Phe Gly Leu Ile Lys Cys Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Asn Ala Leu Leu Lys Lys Tyr Pro Asn Leu Ala Ala Glu Phe 
        995                 1000                1005            
Ile Lys Gly Asp Tyr Arg Arg Asn Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Met His Phe Tyr Gly 
1025                1030                1035                1040
Asn Ile Met Asn Ser Phe Thr Ser Asp Glu Pro Ile Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Ile Ala Thr Val Arg Lys Val Met Asn 
        1075                1080                1085            
Tyr His Gln Val Leu Ile Thr Arg Lys Val Glu Glu Glu Thr Gly Gly 
    1090                1095                1100                
Phe Phe Asp Gln Thr Ile Leu Ser Lys Gly Asn Ser Lys Lys Leu Ile 
1105                1110                1115                1120
Pro Leu Lys Lys Asn Leu Asp Pro Glu Lys Tyr Gly Gly Tyr Asn Ser 
                1125                1130                1135    
Pro Thr Ala Ala Tyr Ser Val Leu Val Glu Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Glu Lys Thr Val Lys Gln Leu Val Pro Ile Pro Val 
        1155                1160                1165            
Arg Glu Lys Ala Ala Leu Glu Lys Asn Pro Ile Lys Tyr Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Glu Asn Pro Lys Val Asn Leu Leu Ile Lys Ile Pro Lys 
1185                1190                1195                1200
Asn Ser Leu Leu Glu Leu Asp Gly Gly Arg Arg Arg Ile Arg Ala Ala 
                1205                1210                1215    
Ala Lys Glu Leu Lys Asn Ala Thr Gln Leu Val Leu Pro Ala Glu Glu 
            1220                1225                1230        
Tyr Thr Leu Leu Glu Lys Val Ala Lys Ile Ala Glu Lys Asn Ser Asn 
        1235                1240                1245            
Ser Glu Ser Ile Ala Lys Lys Glu Gly Tyr Val Glu Glu Leu Leu Ala 
    1250                1255                1260                
Gln Phe Asp Glu Leu Leu Asp Ser Leu Ile Asp Tyr Ser Pro Lys Leu 
1265                1270                1275                1280
Ala Leu Gln Asp Lys Thr Leu Glu Lys Leu Lys Glu Ala Phe Glu Lys 
                1285                1290                1295    
Leu Asn Leu Ala Asp Lys Lys Glu Val Ala Lys Glu Ile Ile Gly Leu 
            1300                1305                1310        
Leu His Cys Thr Ala Thr Thr Ala Asn Thr Ala Leu Lys Ser Leu Gly 
        1315                1320                1325            
Gly Ser Lys Asn Arg Met Arg Tyr Thr Ser Ile Lys Lys Leu Asn Asn 
    1330                1335                1340                
Ala Ser Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 180
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #61

<400> 180
Met Lys Lys Glu Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Lys Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Leu Leu 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Ser Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
His Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Trp Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Gln Glu Glu Ile Ser Lys Val Asp Pro Ser 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Asp Asp Arg His Pro Leu Phe Gly Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Met Glu 
    130                 135                 140                 
Asn Asp Glu Lys His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Ile Glu Gly Asp Leu Ser Ser 
                165                 170                 175     
Lys Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Leu Val Gln Val Phe 
            180                 185                 190         
Asn Asp Ala Leu Glu Glu Glu His Leu Ser Glu Glu Ala Asn Asp Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Arg Ser Ala Arg Ala Lys Glu 
    210                 215                 220                 
Val Val Lys Leu Phe Ala Ser Glu Lys Lys Gln Ala Cys Ile Ser Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Val Gly Leu Lys Gly Asn Leu Lys Lys Val Phe 
                245                 250                 255     
Gly Asp Ser Glu Asp Thr Ala Ile His Phe Ser Lys Asp Asn Tyr Glu 
            260                 265                 270         
Glu Asp Val Glu Ala Ile Arg Asp Thr Ile Pro Asp Glu Tyr Ser Glu 
        275                 280                 285             
Leu Phe Glu Ala Ile Lys Ala Leu Tyr Asp Ala Ile Val Leu Ser Gly 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Asn Asn Thr Glu Ala Lys Val Ser Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Gln His Arg Lys Asp Leu Lys Leu Leu Lys 
                325                 330                 335     
Asn Phe Val Lys Thr Tyr Leu Pro Glu Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Asp Thr Lys Asn Gly Tyr Ser Ala Tyr Ile Lys Gly Gly Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Asn Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Thr Ser Asp Asn Gly Val Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Ala Glu Lys Ile Glu Ser Ile Leu Ala Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Gly Asn Gly Arg Phe Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Glu Asp Gly Val Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Gly Asn Cys Thr Tyr Leu Pro Gly Glu Thr Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Leu Asn Glu Leu Asn Asn Val Arg 
        515                 520                 525             
Leu Thr Ile Asp Lys Gly Lys Trp Arg Glu Arg Phe Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Arg Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Glu Asp Tyr Leu Lys Arg Glu Tyr Gly Glu Phe Asp 
                565                 570                 575     
Ser Ala Asp Ile Ser Gly Ile Asp Gly Glu Phe Lys Ser Ser Leu Gly 
            580                 585                 590         
Ser Tyr His Asp Phe Cys Lys Ile Leu Val Lys Ala Asp Ser Leu Asp 
        595                 600                 605             
Glu Glu Asp Tyr Lys Asp Ile Ile Glu Glu Ile Ile Lys Trp Lys Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Leu Lys Arg Arg Leu Glu Lys Tyr Gly 
625                 630                 635                 640 
Glu Ile Leu Thr Glu Glu Gln Ile Lys Lys Ile Cys Lys Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Val Arg Asp 
            660                 665                 670         
Ala Glu Thr Gly Lys Ser Ile Ile Asp Val Leu Arg Glu Thr Asp Pro 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Leu Ser Asp Glu Asp Leu Gly Phe 
    690                 695                 700                 
Gln Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Glu Asn Leu 
705                 710                 715                 720 
Lys Glu Ile Val Glu Asp Leu Pro Gly Ser Pro Ala Val Lys Arg Gly 
                725                 730                 735     
Ile Asn Gln Ala Leu Arg Ile Val Asp Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Gly His Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Glu Lys Gln 
        755                 760                 765             
Gln Ser Lys Arg Gly Arg Arg Ser Thr Lys Lys Arg Leu Lys Arg Leu 
    770                 775                 780                 
Gln Glu Ala Leu Lys Asn Leu Gly Thr Asn Leu Trp Lys Glu Leu Leu 
785                 790                 795                 800 
Arg Asp Asp Asn Glu Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Met Gly Lys Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Asp Asp 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Val Ser Arg Glu Glu Asn Ala 
    850                 855                 860                 
Arg Lys Thr Asp Asn Leu Pro Ser Pro Glu Ile Arg Arg Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Met Leu Leu Asn Ala Gly Leu Ile Ser Lys Lys Lys 
                885                 890                 895     
Phe Asp Arg Leu Thr Arg Thr Asp Arg Gly Glu Phe Thr Glu Asp Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Thr Leu Leu Lys Gln Arg Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Lys Asn Thr Lys Ile Val Ser Val Lys Ala 
945                 950                 955                 960 
Asn Leu Val Ser Glu Phe Arg Gln Asp Phe Gly Leu Ile Lys Cys Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Asn Ala Leu Leu Lys Lys Tyr Pro Asn Leu Ala Ala Glu Phe 
        995                 1000                1005            
Ile Lys Gly Asp Tyr Arg Arg Asn Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Met His Phe Tyr Gly 
1025                1030                1035                1040
Asn Ile Met Asn Ser Phe Thr Ser Asp Glu Pro Ile Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Ile Ala Thr Val Arg Lys Val Met Asn 
        1075                1080                1085            
Tyr His Gln Val Leu Ile Thr Arg Lys Val Glu Glu Glu Thr Gly Gly 
    1090                1095                1100                
Phe Phe Asp Gln Thr Ile Tyr Ser Lys Gly Asn Ser Lys Lys Leu Ile 
1105                1110                1115                1120
Pro Leu Lys Lys Asn Leu Asp Pro Glu Lys Tyr Gly Gly Tyr Asn Ser 
                1125                1130                1135    
Glu Thr Ala Ala Tyr Ser Val Leu Val Glu Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Glu Lys Thr Val Lys Gln Leu Val Pro Ile Pro Val 
        1155                1160                1165            
Arg Glu Lys Ala Ala Leu Glu Lys Asn Pro Ser Lys Tyr Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Glu Asn Pro Lys Val Asn Leu Leu Ile Lys Ile Pro Lys 
1185                1190                1195                1200
Asn Gln Leu Leu Glu Leu Asp Gly Gly Arg Arg Arg Ile Arg Ala Ala 
                1205                1210                1215    
Ala Lys Glu Leu Lys Asn Ala Thr Gln Leu Val Leu Pro Ala Glu Glu 
            1220                1225                1230        
Tyr Thr Leu Leu Glu Lys Val Ala Lys Ile Ala Glu Lys Asn Ser Asn 
        1235                1240                1245            
Ser Glu Ser Ile Ala Lys Lys Glu Gly Tyr Val Glu Glu Leu Leu Ala 
    1250                1255                1260                
Gln Phe Asp Glu Leu Leu Asp Ser Leu Ile Asp Tyr Ser Pro Lys Leu 
1265                1270                1275                1280
Ala Leu Gln Asp Lys Thr Leu Glu Lys Leu Lys Glu Ala Phe Glu Lys 
                1285                1290                1295    
Leu Asn Leu Ala Asp Lys Lys Glu Val Ala Lys Glu Ile Ile Gly Leu 
            1300                1305                1310        
Leu His Cys Thr Ala Thr Thr Ala Asn Thr Ala Leu Lys Ser Leu Gly 
        1315                1320                1325            
Gly Ser Lys Asn Arg Met Arg Tyr Thr Ser Ile Lys Lys Leu Asn Asn 
    1330                1335                1340                
Ala Ser Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 181
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #62

<400> 181
Thr Thr Asp Glu Tyr Ser Ile Gly Leu Asp Met Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Lys Asn Gly Ser Met Ala His Lys Lys Met 
            20                  25                  30          
Pro Thr Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Pro Thr Leu 
        35                  40                  45              
Gly Ser Arg Leu Phe Asp Ala Ala Glu Thr Ala Ala Glu Ala Arg Ala 
    50                  55                  60                  
His Arg Gly Gln Arg Arg Arg Tyr Val Arg Arg Arg Trp Arg Leu Asp 
65                  70                  75                  80  
Leu Leu Gln Gly Leu Phe Gln Asp Glu Ile Asn Lys Val Asp Ser Glu 
                85                  90                  95      
Phe Phe Ile Arg Leu Arg Gln Ser Arg Leu His Val Glu Asp Arg Ala 
            100                 105                 110         
Asp Tyr Arg Trp Pro Leu Phe Asn Asp Thr Asp Thr Glu Lys Asp Tyr 
        115                 120                 125             
Tyr Lys Arg Phe Pro Thr Ile Tyr His Leu Arg Lys Trp Leu Met Glu 
    130                 135                 140                 
Thr Asp Glu Lys Ala Asp Ile Arg Leu Ile Tyr Leu Ala Val His Asn 
145                 150                 155                 160 
Ile Val Lys His Arg Gly Asn Phe Leu Arg Glu Lys Asn Leu Ser Ser 
                165                 170                 175     
Arg Asn Ala Asp Pro Ala Glu Ala Val Arg His Leu Arg Gln Ala Leu 
            180                 185                 190         
Asn Asp Trp Leu Glu Arg Gly Tyr Leu Ala Glu Ala Asp Asn His Ile 
        195                 200                 205             
Leu Asp Ile Leu Thr Asp Ser Lys Arg Arg Ser Asp Gln Ala Lys Glu 
    210                 215                 220                 
Ile Ala Lys Leu Phe Gly Thr Glu Gln Ser Lys Ala Cys Asn Ser Ala 
225                 230                 235                 240 
Leu Ala Lys Ala Ile Val Gly Leu Lys Ala Glu Leu Lys Asn Val Phe 
                245                 250                 255     
Gly Glu Ala Glu Lys Thr Ala Ile His Leu Ser Ser Asp Asn Tyr Glu 
            260                 265                 270         
Asp Asp Val Glu Ala Ile Arg Asp Ala Ile Pro Asp Asp Ser Gly Glu 
        275                 280                 285             
Leu Phe Gln Ala Leu Cys Ala Leu Tyr Ser Ala Tyr Val Leu Gln Gly 
    290                 295                 300                 
Leu Leu Ser Tyr Ala Pro Gly Gln Thr Glu Ala Lys Ile Ser Ala Asn 
305                 310                 315                 320 
Met Val Ala Lys Tyr Asp Gln Tyr Gly Lys Asp Leu Lys Leu Leu Lys 
                325                 330                 335     
Asn Leu Ala Arg Thr Tyr Val Ser Glu Lys Tyr Asp Ala Phe Phe Arg 
            340                 345                 350         
Gly Glu Thr Tyr Glu Gly Ser Ser Asp Tyr Val Gln Gly Tyr Ala Ser 
        355                 360                 365             
Tyr Asp Asp Phe Gln Lys Ser Val Lys Lys Leu Phe Lys Gln Leu Glu 
    370                 375                 380                 
Glu Asp Arg Tyr Met Met Lys Ala Phe Gly Glu Glu Lys Phe Leu Arg 
385                 390                 395                 400 
Arg Leu Lys Thr Ser Asp Asn Gly Ser Ile Tyr Tyr Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys His Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Glu Glu Glu Lys Ile Glu Ser Leu Val Ser Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Glu Asn Glu Arg Phe Ala Trp 
    450                 455                 460                 
Ser Ser Arg Met Glu Asp Ala Val Ile Thr Pro Trp Asn Trp Asp Glu 
465                 470                 475                 480 
Ile Ile Asp Arg Asp Lys Ser Ala Glu Glu Phe Ile Leu Arg Met Thr 
                485                 490                 495     
Gly Asp Cys Thr Tyr Leu Gln Gly Glu Pro Val Leu Pro Lys Cys Ser 
            500                 505                 510         
Leu Leu Tyr Glu Glu Phe Cys Val Leu Asn Glu Leu Asn Gly Ala Arg 
        515                 520                 525             
Trp Thr Ile Asp Glu Asp Asp Trp Arg Glu Arg Phe Asp Ala Ala Gln 
    530                 535                 540                 
Arg Glu Gly Ile Leu His Asp Leu Phe Arg Lys Thr Arg Lys Val Thr 
545                 550                 555                 560 
Tyr Lys Lys Val Ala Asp Trp Leu Glu Arg Glu Tyr Glu Asn Phe Ala 
                565                 570                 575     
Asn Ala His Val Arg Gly Gly Gln Gly Glu Phe Glu Ser Lys Leu Gly 
            580                 585                 590         
Ser Tyr Ile Phe Phe Cys Lys Asp Val Phe Lys Thr Asp Ser Leu Asp 
        595                 600                 605             
Glu Ala Asp Tyr Lys Pro Met Ile Glu Gln Ile Ile Leu Trp Asn Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Asp Ile Leu Lys Arg Lys Ile Glu Ala Tyr Gly 
625                 630                 635                 640 
Glu His Leu Thr Asp Glu Gln Ile Lys Val Ile Cys Lys Lys Arg Phe 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Ile Lys Asp 
            660                 665                 670         
Ala Asp Thr Gly Arg Ser Ile Ile Asp Val Leu Arg Glu Gly Asn Pro 
        675                 680                 685             
Asn Ser Arg Thr Phe Met Glu Val Leu Arg Asp Glu Asp Leu Gly Phe 
    690                 695                 700                 
Gln Lys Lys Ile Asp Asp Phe Asn Lys Asp Tyr Ala Gly Glu Asn Leu 
705                 710                 715                 720 
Met Asp Ile Val Asn Asp Leu Pro Gly Ser Pro Ala Ile Arg Arg Ser 
                725                 730                 735     
Ile Asn Gln Ala Ile Arg Ile Val Asp Glu Ile Val Ser Ile Ala Gly 
            740                 745                 750         
Gly Lys Glu Pro Ser Asn Ile Phe Ile Glu Val Thr Arg Glu Glu Asp 
        755                 760                 765             
Asp Pro Arg Lys Lys Gly Arg Arg Thr Lys Arg Arg Tyr Glu Ala Ile 
    770                 775                 780                 
Lys Asn Ala Leu Glu Asn Phe Lys Thr Asp Leu Trp Lys Glu Leu Leu 
785                 790                 795                 800 
Lys Lys Asp Ser Asn Asn Leu Asp Glu Arg Leu Ser Leu Tyr Phe Met 
                805                 810                 815     
Gln Arg Gly Lys Cys Met Tyr Ser Gly Arg Pro Ile Lys Leu Glu Glu 
            820                 825                 830         
Leu His Lys Tyr Glu Val Asp His Ile Ile Pro Arg Ser Tyr Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Glu Asn Lys Val Leu Val Cys Arg Glu Glu Asn Gln 
    850                 855                 860                 
Arg Lys Thr Asp Thr Leu Leu Asp Pro Ala Ile Arg Arg Lys Met Gly 
865                 870                 875                 880 
Lys Tyr Trp Arg Met Leu His Asn Ala Lys Leu Ile Gly Asp Lys Lys 
                885                 890                 895     
Phe Asn Asn Leu Phe Arg Arg Asp Arg Gly Glu Ile Asp Asp Lys Thr 
            900                 905                 910         
Leu Lys Gly Phe Val Ala Arg Gln Leu Val Glu Thr Ser Gln Met Val 
        915                 920                 925             
Lys Leu Val Gln Thr Leu Leu Glu Ala Arg Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Lys Asn Thr Lys Ile Val Pro Val Lys Ala 
945                 950                 955                 960 
Asn Ile Ser His Asp Leu Arg Glu Ala Ala Gly Leu Val Lys Cys Arg 
                965                 970                 975     
Glu Ala Asn Asp Phe His His Ala His Asp Ala Tyr Leu Ala Cys Arg 
            980                 985                 990         
Val Gly Leu Phe Ile Gln Lys Arg His Pro Ser Val Tyr Ala Asn His 
        995                 1000                1005            
Ile Met Arg Asp Tyr Ala Arg Lys Gln Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Tyr Asn Lys Thr His Ser Met Ala His Thr Lys Gly 
1025                1030                1035                1040
Phe Ile Val Asn Ser Phe Met Ser Ser Glu Phe Asp Lys Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Phe Trp Asp Ala Glu Ala Glu Val Glu Asp Ile Arg Arg Val Leu Asn 
        1075                1080                1085            
Tyr Arg Gln Cys Phe Ile Ser Arg Met Pro Val Glu Asp Thr Gly Ala 
    1090                1095                1100                
Phe Trp Asp Gln Thr Ile Tyr Ser Pro Arg Ser Asn Lys Lys Leu Leu 
1105                1110                1115                1120
Pro Leu Lys Arg Asp Leu Asp Pro Asp Lys Tyr Gly Ser Tyr Ser Arg 
                1125                1130                1135    
Glu Gln Phe Ala Tyr Ser Val Phe Phe Ile Tyr Asp Ile Glu Ala Arg 
            1140                1145                1150        
Asp Ala Lys Lys Glu Lys Thr Val Leu Gln Phe Ala Gln Val Pro Val 
        1155                1160                1165            
Arg Val Thr Ala Ala Ile Ala Lys Asp Pro Ser Lys Tyr Ala Ser Lys 
    1170                1175                1180                
Leu Gly Gln Glu Asn Leu Glu Phe Thr Arg Ile Ile Arg Ile Leu Lys 
1185                1190                1195                1200
Lys Gln Leu Leu Glu Leu Asp Asn Gly Asp Arg Leu Ile Ile Thr Gly 
                1205                1210                1215    
Ala Lys Glu Val Arg Asn Ala Thr Glu Phe Ala Phe Ser Leu Glu Glu 
            1220                1225                1230        
Thr Thr Leu Val Glu Arg Cys Ala Gln Ile Ala Lys Glu Gly Ser Asn 
        1235                1240                1245            
Pro Glu Asp Ile Ala Ser Asp Glu Gly Tyr Val Glu Gln Leu Ser Ala 
    1250                1255                1260                
Gln Leu Asn Glu Leu Phe Ser Ser Leu Ile Glu Arg Gly Pro Lys Leu 
1265                1270                1275                1280
Gly Val Arg Leu His Ala Gln Leu Lys Leu Asp Gln Tyr Leu Arg Lys 
                1285                1290                1295    
Phe Asp Asp Ala Ala Leu Arg Asp Lys Ala Lys Val Ile Ile Gly Leu 
            1300                1305                1310        
Ile Ser Ile Thr Asn Gly Ala Thr Asn Thr Ala Leu Ser Ser Leu Gly 
        1315                1320                1325            
Gly Ser Lys Asn Arg Met Arg Tyr Thr His Asn Lys Leu Leu Asn Asn 
    1330                1335                1340                
Ala Ser Leu Ile Asp Gln Ser Val Thr Gly Met Phe Glu Arg Arg Thr 
1345                1350                1355                1360
His Ile Gly Leu Leu Gly Glu Asp 
                1365            

<210> 182
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #63

<400> 182
Met Lys Lys Glu Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Lys Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Leu Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Ser Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
His Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Gln Glu Glu Ile Ser Lys Val Asp Pro Ser 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Asp Asp Arg His Pro Leu Phe Gly Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Met Glu 
    130                 135                 140                 
Asn Asp Glu Lys His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Ile Glu Gly Asp Leu Ser Ser 
                165                 170                 175     
Lys Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Leu Val Gln Val Phe 
            180                 185                 190         
Asn Asp Ala Leu Glu Glu Glu His Leu Ser Glu Glu Ala Ile Asp Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Arg Ser Ala Arg Ala Lys Glu 
    210                 215                 220                 
Val Val Lys Leu Phe Ala Ser Glu Lys Lys Gln Ala Cys Ile Ser Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Val Gly Leu Lys Gly Asn Leu Lys Lys Val Phe 
                245                 250                 255     
Gly Asp Ser Glu Asp Thr Ala Ile His Phe Ser Lys Asp Asn Tyr Glu 
            260                 265                 270         
Glu Asp Val Glu Ala Ile Arg Asp Thr Ile Pro Asp Glu Tyr Ser Glu 
        275                 280                 285             
Leu Phe Glu Ala Ile Lys Ala Leu Tyr Asp Ala Ile Val Leu Ser Gly 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Asn Asn Thr Glu Ala Lys Val Ser Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Gln His Arg Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Asn Phe Val Lys Thr Tyr Leu Pro Glu Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Asp Thr Lys Asn Gly Tyr Ser Ala Tyr Ile Lys Gly Gly Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Asn Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Thr Ser Asp Asn Gly Val Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Lys Asn Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Ser Ile Leu Ala Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Gly Asn Gly Arg Phe Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Glu Asp Gly Val Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Asn Cys Thr Tyr Leu Pro Gly Glu Thr Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Leu Asn Glu Leu Asn Asn Val Arg 
        515                 520                 525             
Leu Thr Ile Asp Lys Gly Lys Trp Arg Glu Arg Phe Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Arg Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Glu Asp Tyr Leu Lys Arg Glu Tyr Gly Glu Phe Asp 
                565                 570                 575     
Ser Ala Asp Ile Ser Gly Ile Asp Gly Glu Phe Lys Ser Ser Leu Gly 
            580                 585                 590         
Ser Tyr His Asp Phe Cys Lys Ile Leu Val Lys Ala Asp Ser Leu Asp 
        595                 600                 605             
Glu Glu Asp Tyr Lys Asp Ile Ile Glu Glu Ile Ile Lys Trp Lys Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Leu Lys Arg Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Glu Ile Leu Thr Glu Glu Gln Ile Lys Lys Ile Cys Lys Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Leu Thr Gly Val Arg Asp 
            660                 665                 670         
Lys Glu Thr Gly Lys Ser Ile Ile Asp Val Leu Arg Glu Thr Asp Pro 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Leu Ser Asp Glu Asp Leu Gly Phe 
    690                 695                 700                 
Gln Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Glu Asn Leu 
705                 710                 715                 720 
Lys Glu Ile Val Glu Asp Leu Pro Gly Ser Pro Ala Val Lys Arg Gly 
                725                 730                 735     
Ile Asn Gln Ala Leu Arg Ile Val Asp Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Gly His Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Glu Lys Gln 
        755                 760                 765             
Gln Ser Lys Arg Gly Arg Arg Ser Thr Lys Lys Arg Leu Lys Arg Leu 
    770                 775                 780                 
Gln Glu Ala Leu Lys Asn Leu Gly Thr Asn Leu Trp Lys Glu Leu Leu 
785                 790                 795                 800 
Arg Asp Asp Asn Glu Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Met Gly Lys Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Asp Asp 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Val Ser Arg Glu Glu Asn Ala 
    850                 855                 860                 
Arg Lys Thr Asp Asn Leu Pro Ser Pro Glu Ile Arg Arg Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Met Leu Leu Asn Ala Gly Leu Ile Ser Lys Lys Lys 
                885                 890                 895     
Phe Asp Arg Leu Thr Arg Thr Asp Arg Gly Glu Phe Thr Glu Asp Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Thr Leu Leu Lys Gln Arg Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Lys Asn Thr Lys Ile Val Ser Val Lys Ala 
945                 950                 955                 960 
Asn Leu Val Ser Glu Phe Arg Gln Asp Phe Gly Leu Ile Lys Cys Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Asn Ala Leu Leu Lys Lys Tyr Pro Asn Leu Ala Ala Glu Phe 
        995                 1000                1005            
Ile Lys Gly Asp Tyr Arg Arg Asn Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Met His Phe Tyr Gly 
1025                1030                1035                1040
Asn Ile Met Asn Ser Phe Thr Ser Asp Glu Pro Ile Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Ile Ala Thr Val Arg Lys Val Met Asn 
        1075                1080                1085            
Tyr His Gln Val Leu Ile Thr Arg Lys Val Glu Glu Glu Thr Gly Gly 
    1090                1095                1100                
Phe Phe Asp Gln Thr Ile Leu Ser Lys Gly Asn Ser Lys Lys Leu Ile 
1105                1110                1115                1120
Pro Leu Lys Lys Asn Leu Asp Pro Glu Lys Tyr Gly Gly Tyr Asn Ser 
                1125                1130                1135    
Pro Thr Ala Ala Tyr Ser Val Leu Val Glu Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Glu Lys Thr Val Lys Gln Leu Val Pro Ile Pro Val 
        1155                1160                1165            
Arg Glu Lys Ala Ala Leu Glu Lys Asn Pro Ile Lys Tyr Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Glu Asn Pro Lys Val Asn Leu Leu Ile Lys Ile Pro Lys 
1185                1190                1195                1200
Asn Ser Leu Leu Glu Leu Asp Gly Gly Arg Arg Arg Ile Arg Ala Ala 
                1205                1210                1215    
Ala Lys Glu Leu Lys Asn Ala Thr Gln Leu Val Leu Pro Ala Glu Glu 
            1220                1225                1230        
Tyr Thr Leu Leu Glu Lys Val Ala Lys Ile Ala Glu Lys Asn Ser Asn 
        1235                1240                1245            
Ser Glu Ser Ile Ala Lys Lys Glu Gly Tyr Val Glu Glu Leu Leu Ala 
    1250                1255                1260                
Gln Phe Asp Glu Leu Leu Asp Ser Leu Ile Asp Tyr Ser Pro Lys Leu 
1265                1270                1275                1280
Ala Leu Gln Asp Lys Thr Leu Glu Lys Leu Lys Glu Ala Phe Glu Lys 
                1285                1290                1295    
Leu Asn Leu Ala Asp Lys Lys Glu Val Ala Lys Glu Ile Ile Gly Leu 
            1300                1305                1310        
Leu His Cys Thr Ala Thr Thr Ala Asn Thr Ala Leu Lys Ser Leu Gly 
        1315                1320                1325            
Gly Ser Lys Asn Arg Met Arg Tyr Thr Ser Ile Lys Lys Leu Asn Asn 
    1330                1335                1340                
Ala Ser Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 183
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #64

<400> 183
Met Lys Lys Glu Tyr Tyr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Val Lys Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Thr Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Tyr Arg Thr Ser Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ser Glu Glu Ile Ser Lys Val Asp Pro Ser 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Glu Asp Arg Tyr Pro Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Met Glu 
    130                 135                 140                 
Asn Asp Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Ile Glu Gly Asp Leu Ser Ser 
                165                 170                 175     
Lys Asn Thr Asp Leu Gly Glu Val Phe Ser Gln Leu Val Lys Val Phe 
            180                 185                 190         
Asn Asp Ala Leu Glu Ile Glu Leu Leu Ser Glu Glu Ala Leu Glu Ile 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Lys Ser Ala Lys Ala Lys Glu 
    210                 215                 220                 
Val Val Lys Leu Phe Ala Ser Glu Lys Lys Gln Ala Cys Ile Lys Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Val Gly Leu Thr Gly Asn Leu Ser Glu Ile Phe 
                245                 250                 255     
Gly Asp Ser Glu Asp Thr Ser Ile Asn Phe Ser Asp Ser Asn Tyr Glu 
            260                 265                 270         
Glu Asn Val Glu Ala Ile Glu Asp Thr Ile Pro Asp Glu Phe Ser Glu 
        275                 280                 285             
Leu Ile Glu Ala Ile Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Gly 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Thr Tyr Leu Pro Glu Glu Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Lys Asn Gly Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Arg Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Gln Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Ser Ile Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Gly Asn Gly Ser Asn Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Glu Asp Gly Lys Ile Tyr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Val Val Asp Val Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Asp Lys Ile Lys Trp Arg Glu Arg Phe Ser Val Glu Leu 
    530                 535                 540                 
Lys Gln Glu Ile Tyr Asn Asp Leu Phe Cys Arg Asn Arg Lys Val Thr 
545                 550                 555                 560 
Gly Lys Lys Leu Lys Asp Tyr Leu Lys Arg Glu Tyr Gly Glu Leu Asp 
                565                 570                 575     
Glu Ala Asp Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ser Leu Gly 
            580                 585                 590         
Ser Tyr His Asp Phe Lys Lys Ile Leu Gly Lys Gly Asn Ser Leu Asp 
        595                 600                 605             
Glu Glu Asp Tyr Lys Asp Ile Ile Glu Glu Ile Ile Lys Trp Lys Thr 
    610                 615                 620                 
Ile Phe Asp Asp Lys Lys Met Leu Lys Arg Arg Leu Lys Lys Tyr Pro 
625                 630                 635                 640 
Glu Ile Leu Ser Glu Glu Gln Ile Lys Lys Ile Cys Lys Leu Lys Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Thr Gly Val Arg Asp 
            660                 665                 670         
Lys Glu Thr Gly Glu Ser Ile Ile Asn Ala Leu Trp Glu Thr Asp Pro 
        675                 680                 685             
Ser Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Gln Asp Tyr Gly Phe 
    690                 695                 700                 
Gln Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Glu Asn Ser 
705                 710                 715                 720 
Tyr Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Ala 
                725                 730                 735     
Ile Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Gly His Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Glu Lys Gln 
        755                 760                 765             
Gln Asn Lys Arg Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu 
    770                 775                 780                 
Tyr Lys Ser Cys Lys Asn Asp Ala Thr Asn Leu Trp Lys Glu Leu Leu 
785                 790                 795                 800 
Arg Asp Asp Asn Glu Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Met Gly Lys Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Asp Asp 
            820                 825                 830         
Leu Phe Asn Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Val Arg Lys Glu Leu Asn Ala 
    850                 855                 860                 
Lys Lys Thr Asp Asn Leu Pro Ser Pro Glu Ile Arg Glu Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Met Leu Leu Asn Lys Gly Phe Ile Ser Lys Lys Lys 
                885                 890                 895     
Tyr Asp Arg Leu Thr Arg Thr Thr Arg Gly Glu Phe Thr Glu Asp Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr 
        915                 920                 925             
Lys Ala Val Ala Glu Leu Leu Lys Gln Leu Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Lys Asn Thr Glu Ile Val Tyr Val Lys Ala 
945                 950                 955                 960 
Asn Leu Val Ser Glu Phe Arg Gln Asp Phe Asp Leu Ile Lys Cys Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val 
            980                 985                 990         
Val Gly Asn Val Tyr His Val Lys Tyr Thr Asn Asn Ala Ala Lys Phe 
        995                 1000                1005            
Ile Lys Gly Asn Arg Gly Arg Asn Tyr Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Met His Phe Tyr Gly 
1025                1030                1035                1040
Asn Ile Lys Lys Met Phe Thr Ser Asp Val Pro Ile Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Arg Asn Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Asp Lys Ser Ile Ala Thr Val Arg Lys Val Met Asn 
        1075                1080                1085            
Lys Asn Asn Ile Leu Tyr Thr Arg Tyr Thr Tyr Glu Glu Lys Gly Gly 
    1090                1095                1100                
Leu Phe Asp Gln Thr Ile Leu Lys Lys Gly Asn Ser Gln Lys Leu Ile 
1105                1110                1115                1120
Pro Leu Lys Lys Asn Leu Asp Pro Glu Lys Tyr Gly Gly Tyr Asn Ser 
                1125                1130                1135    
Pro Thr Ala Ala Tyr Phe Ala Leu Val Glu Tyr Asp Ile Glu Glu Gly 
            1140                1145                1150        
Lys Lys Lys Lys Glu Lys Thr Thr Lys Gln Leu Val Pro Ile Pro Val 
        1155                1160                1165            
Tyr Glu Lys Ala Lys Leu Glu Ser Asn Ala Ile Lys Tyr Leu Glu Glu 
    1170                1175                1180                
Arg Gly Leu Lys Asn Pro Lys Val Asn Leu Leu Ile Lys Ile Lys Lys 
1185                1190                1195                1200
Asn Ser Leu Leu Glu Met Asp Gly Tyr Arg Met Arg Ile Arg Gly Ala 
                1205                1210                1215    
Thr Ile Ile Phe Lys Asn Ala Val Gln Leu Val Leu Asp Glu Glu Asn 
            1220                1225                1230        
Tyr Lys Ile Leu Lys Lys Val Ala Lys Val Leu Glu Lys Asn Ser Glu 
        1235                1240                1245            
Asn Gln Thr Ile Ala Lys Lys Glu Gly Tyr Thr Glu Glu Leu Leu Ala 
    1250                1255                1260                
Leu Tyr Glu Glu Leu Leu Asp Lys Leu Lys Asp Tyr Lys Asn Arg Leu 
1265                1270                1275                1280
Ala Asn Gln Ala Lys Thr Leu Glu Lys Gly Lys Glu Ala Phe Glu Lys 
                1285                1290                1295    
Leu Asn Leu Glu Asp Lys Ala Glu Val Ile Asn Glu Ile Ile Arg Leu 
            1300                1305                1310        
Phe His Cys Asn Ala Thr Thr Ala Asn Asn Ala Leu Lys Leu Ile Gly 
        1315                1320                1325            
Gly Ser Lys Asn Arg Met Thr Asn Asn Asn Ile Ser Lys Ser Asn Lys 
    1330                1335                1340                
Val Ser Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Lys Ile 
1345                1350                1355                1360
Asp Leu Gly Lys Ile Gly Glu Asp 
                1365            

<210> 184
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #65

<400> 184
Met Lys Lys Glu Tyr Tyr Leu Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Val Lys Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Ile Arg Leu Phe Asp Thr Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Tyr Arg Ser Ser Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ser Glu Glu Ile Ala Lys Val Asp Pro Thr 
                85                  90                  95      
Phe Phe Ile Arg Leu Asn Glu Ser Arg Leu His Pro Glu Asp Lys Thr 
            100                 105                 110         
Glu Asp Lys Tyr Pro Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Ile Glu 
    130                 135                 140                 
Asn Pro Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Ile Glu Gly Asp Ser Asp Ser 
                165                 170                 175     
Lys Asn Thr Asp Leu Ser Glu Val Phe Ser Glu Leu Val Lys Val Phe 
            180                 185                 190         
Asn Asp Ala Leu Glu Ile Glu Leu Leu Ser Thr Glu Ala Leu Glu Leu 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Lys Ser Lys Ser Ala Lys Ala Lys Glu 
    210                 215                 220                 
Val Ala Lys Leu Phe Ala Asp Glu Lys Glu Gln Gln Cys Ile Lys Ala 
225                 230                 235                 240 
Leu Ser Lys Leu Ile Val Gly Leu Thr Gly Asn Ile Ser Glu Ile Phe 
                245                 250                 255     
His Gln Ser Glu Asp Thr Ser Ile Asn Phe Ser Asp Ser Asn Tyr Glu 
            260                 265                 270         
Glu Asn Val Glu Ala Ile Glu Asp Thr Ile Pro Asp Glu Phe Ser Glu 
        275                 280                 285             
Leu Ile Asp Ala Ile Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Gly 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Ala Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asn Leu Lys Gln Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Thr Tyr Leu Pro Glu Glu Tyr Lys Glu Phe Phe Ser 
            340                 345                 350         
Ser Asp Glu Lys Asn Gly Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Glu Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Lys Glu Lys Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Ala Glu Ile Glu Asn Glu Thr Leu Leu Pro 
385                 390                 395                 400 
Leu Gln Arg Ser Lys Asp Asn Gly Thr Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Gln Glu Leu Lys Lys Ile Leu Glu Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Ile Ser Ile Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ser Arg Gly Asn Gly Ser Asn Ala Trp 
    450                 455                 460                 
Met Ile Arg Lys Glu Asp Gly Lys Ile Tyr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Val Val Asp Val Glu Lys Ser Asn Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Lys 
        515                 520                 525             
Ile Arg Gly Asn Lys Ile Lys Trp Arg Glu Arg Phe Ser Val Glu Leu 
    530                 535                 540                 
Lys Gln Glu Ile Tyr Asn Asp Leu Phe Cys Arg Asn Arg Lys Val Thr 
545                 550                 555                 560 
Gly Lys Lys Ile Leu Asp Tyr Leu Lys Arg Glu Tyr Gly Glu Leu Asp 
                565                 570                 575     
Glu Ala Asp Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ala Leu Ala 
            580                 585                 590         
Ser Tyr His Asp Phe Lys Lys Leu Leu Gly Lys Gly Asn Ser Leu Asp 
        595                 600                 605             
Glu Glu Asp Tyr Lys Glu Ile Ile Glu Glu Ile Ile Lys Trp Lys Thr 
    610                 615                 620                 
Ile Tyr Asp Asp Asn Lys Met Leu Lys Arg Met Leu Lys Lys Tyr Pro 
625                 630                 635                 640 
Glu Ile Leu Ser Glu Glu Gln Ile Lys Lys Ile Cys Lys Leu Lys Tyr 
                645                 650                 655     
Thr Gly Trp Gly Asn Phe Ser Arg Glu Phe Leu Thr Gly Val Arg Gly 
            660                 665                 670         
Lys Glu Thr Gly Glu Ser Ile Ile Asn Ala Leu Trp Glu Thr Asp Pro 
        675                 680                 685             
Ser Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Gln Asp Tyr Ser Phe 
    690                 695                 700                 
Gln Glu Glu Ile Asp Gln Ile Asn Ala Glu Lys Ala Gly Glu Asn Ser 
705                 710                 715                 720 
Tyr Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Asn Lys Arg Ala 
                725                 730                 735     
Ile Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Gly His Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Gly Lys Glu 
        755                 760                 765             
Lys Asn Lys Lys Arg Thr Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu 
    770                 775                 780                 
Tyr Lys Ser Cys Lys Asn Asp Thr Thr Asn Leu Trp Lys Glu Leu Leu 
785                 790                 795                 800 
Arg Asp Asp Asn Glu Leu Arg Ser Lys Lys Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Met Gly Lys Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Asp Asp 
            820                 825                 830         
Leu Leu Asn Trp Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Met Val Leu Val Arg Lys Glu Leu Asn Ala 
    850                 855                 860                 
Lys Lys Thr Asn Asn Leu Leu Ser Pro Glu Ile Arg Glu Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Met Leu Leu Asn Lys Gly Phe Ile Ser Lys Lys Lys 
                885                 890                 895     
Tyr Asp Arg Leu Thr Arg Thr Thr Arg Gly Glu Phe Thr Glu Glu Glu 
            900                 905                 910         
Leu Ser Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr 
        915                 920                 925             
Lys Ala Val Ala Glu Leu Leu Lys Gln Leu Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Lys Asn Ser Glu Val Val Tyr Val Lys Ala 
945                 950                 955                 960 
Asn Leu Val Ser Glu Phe Arg Gln Asp Phe Asp Leu Leu Lys Cys Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val 
            980                 985                 990         
Val Gly Asn Val Tyr His Val Lys Tyr Thr Asn Asn Pro Ala Lys Trp 
        995                 1000                1005            
Ile Lys Gly Asn Arg Asp Ser Asn Tyr Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Met His Phe Tyr Gly 
1025                1030                1035                1040
Ser Ile Lys Lys Met Phe Thr Ser Asp Val Lys Asn Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Arg Asn Gly Glu Ile 
            1060                1065                1070        
Val Trp Asn Lys Asp Lys Ser Ile Asp Gln Ile Arg Lys Ile Met Asn 
        1075                1080                1085            
Lys Asn Asn Ile Leu Tyr Thr Glu Tyr Thr Tyr Cys Glu Lys Gly Gln 
    1090                1095                1100                
Leu Phe Asn Gln Thr Ile Leu Lys Lys Gly Asn Ser Glu Lys Leu Ile 
1105                1110                1115                1120
Pro Leu Lys Lys Asn Leu Asp Pro Glu Lys Tyr Gly Gly Tyr Thr Ser 
                1125                1130                1135    
Pro Lys Ala Ser Tyr Phe Ala Leu Val Glu Phe Asp Ile Glu Glu Gly 
            1140                1145                1150        
Lys Lys Lys Lys Glu Lys Thr Thr Lys Gln Leu Ile Pro Val Pro Val 
        1155                1160                1165            
Tyr Glu Lys Ala Lys Leu Glu Ser Asn Ala Ile Lys Tyr Leu Glu Glu 
    1170                1175                1180                
Arg Gly Leu Thr Asn Val Val Val Asn Leu Lys Ile Lys Ile Lys Lys 
1185                1190                1195                1200
Asn Ser Leu Leu Glu Met Asp Gly Tyr Arg Met Arg Ile Arg Gly Ala 
                1205                1210                1215    
Thr Ile Ile Phe Lys Asn Ala Val Gln Leu Val Leu Asp Glu Glu Asn 
            1220                1225                1230        
Tyr Glu Ile Leu Arg Lys Val Glu Lys Tyr Leu Glu Lys Asn Ser Glu 
        1235                1240                1245            
Asn Gln Thr Ile Ala Lys Lys Glu Gly Phe Thr Glu Glu Leu Leu Ser 
    1250                1255                1260                
Leu Tyr Glu Glu Leu Leu Asp Lys Leu Lys Asp Tyr Lys Asn Arg Pro 
1265                1270                1275                1280
Ala Asn Gln Ala Lys Thr Leu Glu Lys Gly Lys Glu Ala Phe Glu Ser 
                1285                1290                1295    
Leu Asn Leu Glu Asp Lys Ala Glu Val Ile Asn Glu Ile Ile Arg Leu 
            1300                1305                1310        
Phe His Cys Asn Ala Thr Thr Ala Ser Asn Ala Leu Lys Leu Ile Asn 
        1315                1320                1325            
Gly Ser Lys Asn Arg Met Thr Asn Lys Asn Ile Leu Lys Ser Asn Lys 
    1330                1335                1340                
Val Lys Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Lys Ile 
1345                1350                1355                1360
Asp Leu Gly Lys Ile Gly Glu Asp 
                1365            

<210> 185
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #66

<400> 185
Met Lys Lys Glu Tyr Tyr Ile Gly Leu Asp Met Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Leu Cys Lys Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Thr Ala Glu Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ser Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ser Glu Glu Ile Ser Lys Val Asp Pro Ser 
                85                  90                  95      
Phe Phe Leu Arg Leu Asn Glu Ser Lys Leu Tyr Pro Glu Asp Lys Thr 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Glu Leu Met Glu 
    130                 135                 140                 
Asn Asp Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Phe Glu Gly Asp Leu Ser Ser 
                165                 170                 175     
Lys Asn Thr Asp Phe Gly Ala Val Phe Ser Gln Leu Leu Lys Val Ile 
            180                 185                 190         
Asn Asp Glu Leu Glu Ile Glu Leu Leu Ser Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Lys Ser Lys Ser Ala Lys Ala Thr Glu 
    210                 215                 220                 
Val Val Lys Leu Leu Ala Ser Glu Lys Lys Gln Ala Cys Ile Lys Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Ser Gly Leu Thr Val Lys Leu Ser Glu Ile Phe 
                245                 250                 255     
Gly Asp Gly Glu Arg Thr Ser Ile Ser Phe Ala Asp Ser Asn Tyr Glu 
            260                 265                 270         
Glu Asn Val Glu Ala Ile Glu Asp Thr Leu Pro Glu Arg Phe Ser Glu 
        275                 280                 285             
Leu Ile Glu Ala Ile Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Thr Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Thr Tyr Leu Pro Glu Glu Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Lys Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Asn Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Ser Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Ile Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Arg Gly Asn Gly Glu Asn Ala Trp 
    450                 455                 460                 
Met Val Arg Lys Glu Asp Gly Lys Ile Tyr Pro Trp Asn Phe Asp Asn 
465                 470                 475                 480 
Val Val Asp Val Glu Lys Ser Ala Glu Glu Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asn Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Glu Lys Ile Lys Trp Arg Glu Arg Phe Ser Val Glu Leu 
    530                 535                 540                 
Lys Gln Glu Ile Tyr Asn Asp Leu Phe Cys Arg Asn Arg Lys Val Thr 
545                 550                 555                 560 
Gly Lys Lys Leu Lys Asp Tyr Leu Lys Arg Glu Gly Gly Glu Leu Lys 
                565                 570                 575     
Glu Ala Glu Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ser Leu Arg 
            580                 585                 590         
Ser Tyr His Asp Phe Lys Lys Ile Leu Gly Lys Gly Asn Ser Leu Asp 
        595                 600                 605             
Glu Glu Asp Thr Lys Asp Ile Ile Glu Glu Ile Ile Lys Asn Ile Thr 
    610                 615                 620                 
Ile Phe Asp Asp Lys Lys Met Leu Lys Arg Arg Leu Lys Lys Tyr Pro 
625                 630                 635                 640 
Glu Ile Leu Ser Glu Glu Gln Ile Lys Lys Ile Cys Lys Leu Lys Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Thr Glu Val Arg Asp 
            660                 665                 670         
Lys Glu Thr Gly Glu Asn Ile Ile Asn Ala Leu Trp Glu Thr Asp Pro 
        675                 680                 685             
Ser Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Gln Asp Tyr Gly Phe 
    690                 695                 700                 
Gln Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Glu Asn Ser 
705                 710                 715                 720 
Tyr Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Ala 
                725                 730                 735     
Ile Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Gly His Glu Pro Lys Arg Ile Phe Val Glu Met Ala Arg Glu Lys Gln 
        755                 760                 765             
Gln Asn Lys Arg Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu 
    770                 775                 780                 
Tyr Lys Ser Cys Lys Asn Asp Ala Thr Asp Leu Trp Lys Glu Leu Leu 
785                 790                 795                 800 
Arg Asp Asp Asn Glu Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Met Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp 
            820                 825                 830         
Leu Phe Asn Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Val Lys Lys Glu Leu Asn Ala 
    850                 855                 860                 
Lys Lys Thr Asp Asn Tyr Pro Ser Pro Glu Ile Arg Glu Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Met Leu Leu Asn Lys Gly Phe Ile Ser Lys Lys Lys 
                885                 890                 895     
Tyr Asp Arg Leu Thr Arg Thr Thr Arg Gly Glu Phe Thr Glu Asp Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr 
        915                 920                 925             
Lys Ala Val Ala Glu Leu Leu Lys Gln Leu Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Lys Asn Thr Glu Ile Val Tyr Val Lys Ala 
945                 950                 955                 960 
Asn Leu Val Ser Glu Phe Arg Gln Asp Phe Asp Phe Ile Lys Cys Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val 
            980                 985                 990         
Val Gly Asn Val Tyr His Val Lys Tyr Thr Asn Asn Ala Ala Lys Phe 
        995                 1000                1005            
Ile Lys Gly Asn Pro Gly Arg Asn Tyr Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Met His Phe Tyr Gly 
1025                1030                1035                1040
Asn Leu Lys Lys Met Phe Thr Ser Asp Val Pro Ile Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Arg Asn Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Asp Lys Ser Ile Ala Thr Val Arg Lys Val Met Asn 
        1075                1080                1085            
Lys Asn Asn Ile Leu Tyr Thr Arg Tyr Ser Tyr Glu Glu Lys Gly Gly 
    1090                1095                1100                
Leu Phe Asp Gln Gln Ile Leu Lys Lys Gly Asn Gly Gln Ala Leu Ile 
1105                1110                1115                1120
Pro Leu Lys Lys Arg Leu Asp Ile Glu Lys Tyr Gly Gly Tyr Asn Lys 
                1125                1130                1135    
Pro Thr Ala Ala Tyr Phe Ala Leu Val Glu Tyr Asp Ile Glu Glu Gly 
            1140                1145                1150        
Lys Lys Gly Lys Glu Lys Thr Thr Lys Gln Leu Val Pro Ile Pro Val 
        1155                1160                1165            
Tyr Glu Lys Ala Lys Leu Glu Ser Asn Ala Ile Lys Tyr Leu Glu Glu 
    1170                1175                1180                
Arg Gly Leu Lys Asn Pro Lys Val Asn Leu Leu Ile Lys Ile Lys Lys 
1185                1190                1195                1200
Asn Ser Leu Leu Glu Val Asp Gly Phe Arg Met His Ile Ser Gly Arg 
                1205                1210                1215    
Thr Ile Ile Phe Lys Ser Ala Val Gln Leu Val Leu Asp Glu Glu Asn 
            1220                1225                1230        
Glu Lys Ile Leu Lys Lys Val Ala Lys Val Leu Glu Lys Gln Ser Glu 
        1235                1240                1245            
Asn Gln Lys Ile Ala Lys Lys Glu Gly Tyr Thr Glu Glu Leu Leu Ala 
    1250                1255                1260                
Leu Tyr Glu Glu Leu Leu Asp Lys Leu Lys Asp Tyr Lys Ile Arg Leu 
1265                1270                1275                1280
Ala Asn Gln Ala Lys Thr Leu Glu Asn Gly Lys Glu Lys Phe Glu Lys 
                1285                1290                1295    
Leu Asn Leu Glu Asp Lys Cys Gln Val Ile Asn Glu Ile Leu Arg Leu 
            1300                1305                1310        
Phe His Cys Asn Ala Thr Thr Ala Asn Asn Ala Leu Ser Leu Ile Gly 
        1315                1320                1325            
Gly Ala Lys Asn Ile Met Thr Asn Asn Asn Ile Ser Lys Ser Asn Lys 
    1330                1335                1340                
Val Ser Leu Ile Asn Gln Ser Ile Thr Gly Leu Tyr Glu Thr Lys Ile 
1345                1350                1355                1360
Asp Leu Gly Lys Ile Gly Glu Asp 
                1365            

<210> 186
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #67

<400> 186
Met Lys Lys Glu Tyr Tyr Val Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Asn Tyr Asn Ile Cys Lys Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Glu Thr Ala Lys Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ser Arg Arg Arg Leu Gln Arg Arg Lys Gln Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Lys Val Asp Pro Gly 
                85                  90                  95      
Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro Glu Asp Lys Pro 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Glu 
    130                 135                 140                 
Asn Glu Glu Pro His Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Ile Ile Lys His Arg Gly His Phe Leu Phe Glu Gly Asp Ile Ser Ser 
                165                 170                 175     
Lys Val Thr Asp Phe Gly Ala Ala Phe Ser Gln Phe Leu Lys Val Ile 
            180                 185                 190         
Asn Asp Glu Leu Glu Ile Glu Leu Leu Asp Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Lys Ser Lys Ser Ala Lys Lys Thr Glu 
    210                 215                 220                 
Leu Val Lys Leu Leu Ala Ala Glu Lys Lys Gln Ala Cys Glu Lys Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Ser Gly Cys Thr Val Lys Leu Ser Glu Ile Phe 
                245                 250                 255     
Gly Asp Gly Glu Arg Thr Lys Ile Ser Phe Ala Asp Ser Asn Tyr Glu 
            260                 265                 270         
Glu Tyr Val Ala Ala Val Glu Asp Thr Leu Ala Glu Arg Phe Leu Glu 
        275                 280                 285             
Leu Ile Glu Ala Ala Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Tyr Ile Ser Glu Ala Lys Val Lys Thr Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Thr Tyr Leu Pro Glu Glu Tyr Lys Asp Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Lys Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Lys Glu Asp Phe Tyr Ala Phe Leu Lys Lys Asn Leu Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Ile Glu Asn Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Ser Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Ile Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Lys Gly Asn Gly Glu Asn Ala Trp 
    450                 455                 460                 
Ala Val Arg Lys Glu Asp Gly Lys Ile Tyr Pro Trp Asn Phe Glu Asn 
465                 470                 475                 480 
Val Ile Asp Val Glu Glu Ser Ala Glu Lys Phe Ile Gly Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asp Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Glu Lys Ile Lys Trp Arg Glu Arg Phe Ser Val Glu Leu 
    530                 535                 540                 
Lys Gln Glu Ile Tyr Glu Asp Val Phe Cys Arg Tyr Arg Lys Val Thr 
545                 550                 555                 560 
Gly Lys Lys Leu Lys Asp Tyr Leu Ile Arg Glu Gly Ile Ile Gly Lys 
                565                 570                 575     
Glu Ala Glu Ile Ser Gly Ile Asp Gly Asp Phe Lys Ser Ser Leu Arg 
            580                 585                 590         
Ala Tyr His Asp Phe Lys Lys Ile Leu Gly Lys Gly Lys Leu Leu Asp 
        595                 600                 605             
Val Glu Leu Thr Gln Asn Glu Lys Glu Glu Ile Ile Lys Asn Ile Thr 
    610                 615                 620                 
Ile Phe Asp Asp Lys Lys Met Leu Lys Lys Arg Leu Lys Lys Tyr Pro 
625                 630                 635                 640 
Glu Ile Leu Ser Glu Lys Gln Ile Lys Lys Ile Cys Ala Leu Lys Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Glu Glu Val Gln Ala 
            660                 665                 670         
Lys Glu Thr Gly Glu Asn Ile Ile Thr Ala Leu Trp Glu Thr Asp Pro 
        675                 680                 685             
Ser Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Gln Asp Tyr Gly Phe 
    690                 695                 700                 
Gln Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Lys Asn Ser 
705                 710                 715                 720 
Tyr Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Pro 
                725                 730                 735     
Ile Trp Gln Thr Leu Gln Ile Val Glu Glu Ile Glu Lys Val Met Gly 
            740                 745                 750         
Gly His Lys Pro Lys Arg Ile Phe Val Glu Met Ala Arg Glu Lys Gln 
        755                 760                 765             
Gln Asn Lys Arg Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Glu Leu 
    770                 775                 780                 
Tyr Lys Ser Cys Lys Asn Glu Ala Pro Asp Leu Trp Lys Glu Leu Leu 
785                 790                 795                 800 
His Asp Asp Asn Lys Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Met Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp 
            820                 825                 830         
Leu Phe Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Met 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Val Lys Lys Glu Leu Asn Ala 
    850                 855                 860                 
Lys Lys Thr Asp Asn Tyr Pro Ser Ala Glu Ile Arg Glu Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Asp Met Leu Leu Asn Lys Gly Phe Ile Ser Lys Glu Lys 
                885                 890                 895     
Tyr Asp Arg Leu Thr Arg Asn Thr Arg Gly Glu Phe Thr Glu Asn Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Ala Arg Gln Leu Val Glu Thr Arg Gln Ser Thr 
        915                 920                 925             
Lys Ala Val Ala Glu Leu Leu Lys Gln Ala Tyr Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Lys Glu Thr Glu Ile Val Tyr Val Lys Ala 
945                 950                 955                 960 
Asn Thr Val Ser Glu Phe Arg Gln Asp Phe Asp Phe Ile Lys Val Arg 
                965                 970                 975     
Asp Val Asn Asp Tyr His His Ala Lys Asp Ala Tyr Leu Asn Ile Val 
            980                 985                 990         
Val Gly Asn Thr Tyr Tyr Val Lys Phe Thr Lys Asn Ala Ala Trp Phe 
        995                 1000                1005            
Ile Lys Gly Asn Pro Gly Arg Thr Tyr Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Met His Phe Tyr Gly 
1025                1030                1035                1040
Asn Leu Lys Lys Met Phe Thr Ser Asp Val Ala Ile Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Arg Asn Gly Glu Ile 
            1060                1065                1070        
Ala Trp Gly Lys Asp Gly Thr Ile Val Thr Val Arg Lys Val Met Asn 
        1075                1080                1085            
Lys Asn Asn Ile Leu Phe Thr Arg Arg Ser Tyr Glu Val Lys Gly Gly 
    1090                1095                1100                
Leu Phe Asp Gln Gln Ile Met Lys Lys Gly Lys Gly Gln Ala Leu Ile 
1105                1110                1115                1120
Pro Val Lys Glu Arg Leu Ser Ile Glu Lys Tyr Gly Gly Tyr Asn Lys 
                1125                1130                1135    
Ala Thr Gly Ala Tyr Phe Met Leu Val Glu Ser Asp Ile Glu Glu Gly 
            1140                1145                1150        
Lys Lys Gly Lys Glu Lys Thr Thr Lys Gln Leu Val Pro Ile Pro Val 
        1155                1160                1165            
Tyr Glu Lys Ala Lys Leu Glu Ser Asn Ala Ile Arg Tyr Leu Glu Glu 
    1170                1175                1180                
Arg Gly Leu Lys Asn Pro Lys Val Asn Leu Leu Ile Lys Ile Lys Lys 
1185                1190                1195                1200
Asp Ser Leu Phe Glu Val Asp Gly Phe Arg Met His Leu Ser Gly Arg 
                1205                1210                1215    
Thr Leu Ile Phe Lys Cys Ala Asn Gln Leu Val Leu Asp Glu Glu Asn 
            1220                1225                1230        
Glu Lys Ile Leu Lys Lys Val Ala Lys Val Leu Glu Lys Gln Lys Lys 
        1235                1240                1245            
Asn Val Lys Ile Ser Lys Lys Asp Gly Tyr Thr Glu Glu Leu Leu Ala 
    1250                1255                1260                
Lys Tyr Glu Glu Phe Leu Asp Lys Leu Lys Asp Tyr Asn Ile Arg Leu 
1265                1270                1275                1280
Ser Thr Gln Ala Lys Thr Leu Glu Asn Gly Lys Glu Lys Phe Glu Lys 
                1285                1290                1295    
Leu Ser Leu Glu Asp Lys Cys Gln Val Ile Asn Glu Ile Leu His Leu 
            1300                1305                1310        
Phe Gln Cys Gln Ser Thr Ala Ala Asn Asn Ala Leu Ser Leu Ile Gly 
        1315                1320                1325            
Gly Ala Gly Asn Ile Leu Val Asn Asn Asn Ile Ser Lys Cys Asn Lys 
    1330                1335                1340                
Val Ser Ile Ile Asn Gln Ser Ile Thr Gly Leu Tyr Glu Gln Glu Ile 
1345                1350                1355                1360
Asp Leu Lys Lys Ile Gly Glu Asp 
                1365            

<210> 187
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #68

<400> 187
Met Lys Lys Glu Tyr Tyr Val Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Ser Tyr His Ile Cys Arg Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Glu Thr Ala Lys Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ser Arg Arg Arg Leu Asp Arg Arg Lys Arg Arg Ile Glu 
65                  70                  75                  80  
Leu Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Lys Val Asp Pro Gly 
                85                  90                  95      
Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro Glu Asp Lys Pro 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Glu 
    130                 135                 140                 
Asn Glu Glu Pro Pro Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Met Ile Lys His Arg Gly His Phe Leu Phe Ala Gly Asp Ile Ser Glu 
                165                 170                 175     
Lys Val Thr Asp Phe Gly Ala Ala Phe Ser Gln Phe Leu Lys Val Ile 
            180                 185                 190         
Lys Asp Glu Leu Asp Ile Glu Leu Leu Asp Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Leu Thr Arg Ser Ala Lys Lys Thr Glu 
    210                 215                 220                 
Leu Val Lys Gly Leu Lys Ala Glu Lys Lys Asn Ala Cys Glu Lys Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Ser Gly Cys Thr Val Lys Leu Ser Glu Ile Phe 
                245                 250                 255     
Gly Asp Gly Glu Arg Pro Lys Ile Ser Phe Ala Asp Ser Asn Tyr Glu 
            260                 265                 270         
Glu Tyr Val Ala Val Val Glu Asp Glu Leu Gly Glu Arg Phe Leu Glu 
        275                 280                 285             
Ile Ile Glu Ala Ala Lys Ala Leu Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Ser Ile Ser Glu Ala Lys Val Arg Thr Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Arg Lys Asp Leu Lys Tyr Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Thr Tyr Leu Pro Glu Glu Tyr Lys Asp Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Lys Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Lys Glu Asp Phe Tyr Ala Phe Leu Lys Lys Asn Ile Lys Glu Leu Glu 
    370                 375                 380                 
Glu Ala Glu Tyr Leu Leu Glu Glu Leu Glu Lys Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Ser Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Ser Glu Leu Lys Lys Ile Leu Lys Asn Ala Gly Glu Tyr Leu Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Ile Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Lys Gly Asn Gly Glu Phe Ala Trp 
    450                 455                 460                 
Ala Val Arg Lys Ser Asn Glu Lys Ile Tyr Pro Trp Asn Phe Glu Asn 
465                 470                 475                 480 
Val Ile Asp Val Glu Ala Ser Ala Glu Lys Phe Ile Arg Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Pro Gly Glu Asp Val Leu Pro Lys Asp Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Ile Asp Gly Glu Lys Ile Lys Trp Arg Glu Arg Phe Ser Val Glu Leu 
    530                 535                 540                 
Lys Gln Glu Ile Tyr Glu Asp Val Phe Cys Arg Tyr Arg Lys Val Thr 
545                 550                 555                 560 
Ala Lys Lys Leu Lys Asp Tyr Leu Ile Arg Glu Gly Ile Ile Gly Lys 
                565                 570                 575     
Asp Ala Glu Ile Ser Gly Ile Asp Gly Asp Phe Lys Gly Ser Leu Arg 
            580                 585                 590         
Ala Tyr His Asp Phe Lys Lys Ile Leu Gly Lys Gly Lys Leu Leu Asp 
        595                 600                 605             
Val Glu Leu Thr Gln Asn Glu Lys Glu Glu Ile Ile Leu Asn Ile Thr 
    610                 615                 620                 
Leu Phe Asp Asp Lys Lys Leu Leu Lys Lys Arg Leu Lys Lys Tyr Pro 
625                 630                 635                 640 
Gln Ile Leu Thr Glu Lys Gln Ile Lys Lys Ile Cys Ala Leu Asn Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Glu Phe Leu Glu Glu Val Gln Ala 
            660                 665                 670         
Pro Glu Thr Gly Glu Asn Ile Ile Thr Ala Leu Trp Glu Thr Asp Pro 
        675                 680                 685             
Ser Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Gln Asp Tyr Gly Phe 
    690                 695                 700                 
Gln Glu Glu Ile Glu Gln Leu Asn Ala Glu Lys Ala Gly Lys Asn Ser 
705                 710                 715                 720 
Tyr Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Gln 
                725                 730                 735     
Ile Trp Gln Thr Leu Gln Ile Val Lys Glu Ile Glu Lys Val Met Gly 
            740                 745                 750         
Gly Glu Lys Pro Lys Arg Val Phe Val Glu Met Ala Arg Glu Lys Gln 
        755                 760                 765             
Glu Asn Lys Arg Gly Arg Thr Ser Arg Lys Lys Arg Leu Lys Asp Leu 
    770                 775                 780                 
Tyr Lys Ser Cys Lys Asn Glu Glu Pro Asp Trp Trp Lys Glu Leu Leu 
785                 790                 795                 800 
His Glu Glu His Lys Leu Arg Ser Asp Lys Leu Phe Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Lys Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp 
            820                 825                 830         
Leu Trp Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Val Met 
        835                 840                 845             
Asp Asp Ser Leu Asn Asn Arg Val Leu Val Lys Lys Glu Tyr Asn Ala 
    850                 855                 860                 
Lys Lys Thr Asp Asn Tyr Pro Ser Ala Glu Ile Arg Gln Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Asp Ser Leu Leu Asn Gly Gly Phe Ile Ser Lys Glu Lys 
                885                 890                 895     
Tyr Asp Arg Leu Ile Arg Asn Thr Arg Gly Glu Phe Thr Ala Asn Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Glu Arg Gln Leu Val Glu Thr Arg Gln Ser Thr 
        915                 920                 925             
Lys Ala Val Ala Glu Ile Leu Lys Gln Ala Phe Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Lys Glu Thr Glu Ile Val Tyr Val Lys Ala 
945                 950                 955                 960 
Lys Thr Val Ser Glu Phe Arg Gln Asp Phe Asp Phe Ile Lys Val Arg 
                965                 970                 975     
Asp Val Asn Asp Phe His His Ala Lys Asp Ala Tyr Leu Asn Ile Val 
            980                 985                 990         
Val Gly Asn Thr Tyr Tyr Val Lys Phe Thr Lys Asn Ala Ala Trp Phe 
        995                 1000                1005            
Ile Lys Gly Asn Pro Gly Arg Thr Tyr Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Met His Phe Tyr Gly 
1025                1030                1035                1040
Asn Leu Lys Lys Met Phe Thr Ser Asp Val Ala Ile Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Arg Asn Gly Glu Ile 
            1060                1065                1070        
Ala Trp Gly Lys Ser Gly Thr Ile Val Thr Val Arg Lys Met Met Asn 
        1075                1080                1085            
Lys Asn Asn Ile Leu Phe Thr Arg Arg Ser Tyr Glu Val Lys Gly Gly 
    1090                1095                1100                
Leu Phe Asp Gln Gln Ile Met Lys Lys Gly Lys Gly Gln Ala Leu Ile 
1105                1110                1115                1120
Pro Val Lys Glu Arg Leu Ser Ile Glu Lys Tyr Gly Gly Tyr Asn Lys 
                1125                1130                1135    
Ala Thr Gly Ala Tyr Phe Met Leu Val Glu Ser Asp Ile Glu Glu Gly 
            1140                1145                1150        
Lys Lys Gly Lys Glu Lys Thr Thr Ile Glu Phe Val Pro Ile Pro Leu 
        1155                1160                1165            
Tyr Glu Lys Ala Lys Leu Glu Ser Asn Glu Ala Arg Phe Leu Glu Glu 
    1170                1175                1180                
Arg Gly Leu Lys Glu Pro Lys Val Asn Leu Leu Ile Lys Ile Lys Lys 
1185                1190                1195                1200
Asp Thr Leu Phe Glu Val Asp Gly Phe Lys Met His Leu Ser Gly Arg 
                1205                1210                1215    
Thr Leu Ile Phe Lys Cys Ala Asn Gln Leu Ile Leu Asp Glu Glu Asn 
            1220                1225                1230        
Glu Lys Ile Leu Lys Lys Val Ala Lys Val Ile Glu Arg Gln Lys Lys 
        1235                1240                1245            
Asn Val Lys Ile Ser Lys Lys Asp Gly Tyr Thr Glu Glu Leu Leu Ala 
    1250                1255                1260                
Lys Tyr Glu Glu Phe Leu Asp Lys Leu Lys Asn Tyr Gly Ile Arg Leu 
1265                1270                1275                1280
Ser Thr Gln Ala Lys Thr Leu Glu Asn Gly Lys Glu Lys Phe Glu Lys 
                1285                1290                1295    
Leu Ser Leu Glu Asp Lys Cys Gln Val Leu Asn Glu Ile Leu His Leu 
            1300                1305                1310        
Phe Gln Cys Gln Ser Ser Ala Ala Asn Asn Ala Leu Ser Met Ile Gly 
        1315                1320                1325            
Gly Pro Gly Asn Ile Leu Val Asn Asn Asn Ile Ser Lys Cys Asn Lys 
    1330                1335                1340                
Ile Ser Ile Ile Asn Gln Ser Pro Thr Gly Ile Tyr Glu Gln Glu Ile 
1345                1350                1355                1360
Asp Leu Lys Lys Ile Gly Glu Asp 
                1365            

<210> 188
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #69

<400> 188
Met Glu Lys Glu Tyr Tyr Val Gly Leu Asp Met Gly Thr Gly Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Asp Glu Ser Tyr His Ile Leu Arg Arg His Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Gly Lys Ser Lys Gly Lys Ala Met Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Glu Ser Ala Lys Thr Ala Glu Glu Arg Arg Met 
    50                  55                  60                  
Phe Arg Thr Ser Arg Arg Arg Leu Asp Arg Arg Asn Trp Arg Ile Glu 
65                  70                  75                  80  
Ile Leu Gln Glu Ile Phe Ala Glu Glu Ile Ser Lys Val Asp Pro Gly 
                85                  90                  95      
Phe Phe Leu Arg Met Lys Glu Ser Lys Tyr Tyr Pro Glu Asp Lys Pro 
            100                 105                 110         
Glu Leu Pro Tyr Ala Leu Phe Val Asp Lys Glu Thr Asp Lys Asp Tyr 
        115                 120                 125             
His Lys Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Met Leu Met Glu 
    130                 135                 140                 
Thr Glu Glu Thr Pro Asp Ile Arg Leu Val Tyr Leu Ala Leu His His 
145                 150                 155                 160 
Met Met Lys His Arg Gly His Phe Leu Leu Ser Gly Asp Ile Ser Glu 
                165                 170                 175     
Lys Val Thr Glu Phe Gly Thr Thr Phe Ser Gln Phe Leu Glu Asn Ile 
            180                 185                 190         
Lys Asn Glu Leu Asp Phe Glu Leu Leu Asp Glu Glu Ala Leu Gln Ile 
        195                 200                 205             
Glu Glu Ile Leu Lys Asp Lys Leu Thr Arg Ser Ala Lys Lys Thr Lys 
    210                 215                 220                 
Leu Val Lys Gly Leu Lys Ala Glu Lys Lys Asn Val Cys Glu Lys Ala 
225                 230                 235                 240 
Leu Leu Lys Leu Leu Ser Gly Gly Thr Val Lys Leu Ser Asp Ile Phe 
                245                 250                 255     
Gly Met Gly Glu Arg Pro Lys Ile Ser Phe Ala Asp Asn Gly Tyr Glu 
            260                 265                 270         
Glu Tyr Val Ala Val Val Glu Asp Glu Leu Gly Glu Arg Phe Tyr Glu 
        275                 280                 285             
Ile Ile Glu Ala Ala Lys Ala Val Tyr Asp Trp Ala Val Leu Val Asp 
    290                 295                 300                 
Ile Leu Gly Gly Ser Thr Ser Ile Ser Glu Ala Lys Val Arg Thr Ser 
305                 310                 315                 320 
Met Ile Ala Arg Tyr Glu Lys His Lys Ser Asp Leu Lys Tyr Leu Lys 
                325                 330                 335     
Asn Ile Val Lys Lys Tyr Leu Pro Glu Glu Tyr Lys Asp Ile Phe Ser 
            340                 345                 350         
Ser Asp Lys Leu Asn Asn Tyr Ser Ala Tyr Ile Lys Asn Gly Cys Ser 
        355                 360                 365             
Lys Glu Glu Phe Tyr Asp Phe Leu Lys Lys Asn Ile Lys Glu Leu Glu 
    370                 375                 380                 
Glu Gln Glu Tyr Leu Gln Glu Glu Leu Glu Lys Glu Thr Phe Leu Pro 
385                 390                 395                 400 
Lys Gln Val Asn Lys Asp Asn Gly Val Ile Pro Tyr Gln Val His Leu 
                405                 410                 415     
Tyr Glu Leu Lys Lys Ile Leu Asp Asn Leu Gly Asp Lys Ile Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Glu Gln Leu Phe Glu Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Asn Lys Gly Asn Gly Glu Phe Ala Trp 
    450                 455                 460                 
Ala Val Arg Lys Ser Asn Glu Lys Ile Tyr Pro Trp Asn Phe Glu Asn 
465                 470                 475                 480 
Val Ile Asp Val Glu Ala Ser Ala Glu Lys Phe Ile Arg Arg Met Thr 
                485                 490                 495     
Asn Lys Cys Thr Tyr Leu Met Gly Glu Asp Val Leu Pro Lys Asp Ser 
            500                 505                 510         
Leu Leu Tyr Ser Lys Phe Met Val Leu Asn Glu Leu Asn Asn Leu Arg 
        515                 520                 525             
Leu Asp Gly Glu Lys Ile Lys Trp Arg Glu Arg Phe Ser Val Glu Leu 
    530                 535                 540                 
Lys Gln Lys Ile Tyr Glu Asp Val Phe Cys Arg Tyr Arg Lys Val Thr 
545                 550                 555                 560 
Ala Lys Lys Leu Lys Asn Tyr Leu Ile Arg Glu Gly Ile Ile Gly Lys 
                565                 570                 575     
Asn Val Glu Ile Thr Gly Ile Asp Gly Asp Phe Lys Gly Ser Leu Thr 
            580                 585                 590         
Ala Tyr His Asp Phe Lys Lys Ile Leu Gly Lys Gly Lys Leu Leu Asp 
        595                 600                 605             
Val Glu Leu Thr Gln Asn Glu Lys Glu Glu Ile Ile Leu Asn Ile Thr 
    610                 615                 620                 
Leu Phe Asp Asp Lys Lys Leu Leu Lys Lys Arg Leu Lys Lys Tyr Pro 
625                 630                 635                 640 
Gln Ile Leu Thr Glu Lys Gln Ile Lys Lys Ile Cys Ala Leu Ser Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Lys Lys Phe Leu Glu Glu Val Thr Ala 
            660                 665                 670         
Pro Glu Thr Gly Glu Asn Ile Ile Thr Ala Leu Trp Glu Thr Asp Pro 
        675                 680                 685             
Ser Asn Asp Asn Leu Met Gln Leu Leu Ser Asn Glu Asp Tyr Arg Phe 
    690                 695                 700                 
Gln Glu Glu Ile Glu Gln Phe Asn Ala Glu Lys Ala Gly Lys Asn Ser 
705                 710                 715                 720 
Tyr Glu Thr Val Glu Asp Leu Tyr Val Ser Pro Ala Val Lys Arg Gln 
                725                 730                 735     
Ile Trp Gln Thr Leu Gln Ile Val Lys Glu Ile Glu Lys Val Met Gly 
            740                 745                 750         
Gly Glu Lys Pro Lys Arg Val Phe Val Glu Met Ala Arg Glu Lys Gln 
        755                 760                 765             
Glu Asn Lys Arg Gly Arg Glu Ser Arg Lys Lys Arg Leu Ser Asp Leu 
    770                 775                 780                 
Tyr Lys Ser Cys Lys Asn Glu Glu Pro Asp Trp Trp Lys Glu Leu Leu 
785                 790                 795                 800 
His Glu Glu His Lys Leu Arg Ser Asp Lys Leu Tyr Leu Tyr Tyr Thr 
                805                 810                 815     
Gln Lys Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Asp Leu Glu Asp 
            820                 825                 830         
Leu Trp Lys Tyr Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Thr Met 
        835                 840                 845             
Asp Asp Ser Leu Asn Asn Arg Val Leu Val Lys Lys Glu Tyr Asn Ala 
    850                 855                 860                 
Lys Lys Ser Asp Asn Tyr Pro Ser Ala Asp Ile Arg Gln Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Lys Ser Leu Leu Asn Gly Gly Phe Ile Ser Lys Glu Lys 
                885                 890                 895     
Tyr Asp Arg Leu Ile Arg Asn Thr Arg Gly Glu Leu Thr Ala Asn Glu 
            900                 905                 910         
Leu Ala Gly Phe Ile Glu Arg Gln Ile Val Glu Thr Arg Gln Ser Thr 
        915                 920                 925             
Lys Ala Val Ala Glu Ile Leu Lys Gln Val Phe Pro Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Lys Glu Thr Glu Ile Val Tyr Val Lys Ala 
945                 950                 955                 960 
Lys Thr Val Ser Lys Phe Arg Gln Asp Phe Asp Phe Ile Lys Val Arg 
                965                 970                 975     
Asp Val Asn Asp Leu His His Ala Lys Asp Ala Tyr Leu Asn Ile Val 
            980                 985                 990         
Val Gly Asn Thr Tyr Tyr Val Lys Phe Thr Lys Asn Ala Ala Trp Phe 
        995                 1000                1005            
Ile Lys Glu Asn Pro Gly Arg Thr Tyr Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Asn Ala Lys Met His Phe Tyr Gly 
1025                1030                1035                1040
Asn Leu Lys Lys Met Phe Thr Ser Asp Val Ala Ile Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Arg Asn Gly Glu Ile 
            1060                1065                1070        
Ala Trp Gly Lys Ser Gly Thr Ile Val Thr Val Arg Lys Met Met Asn 
        1075                1080                1085            
Lys Asn Asn Ile Leu Val Thr Arg Arg Ser Tyr Glu Val Lys Gly Gly 
    1090                1095                1100                
Leu Phe Asp Gln Gln Ile Met Lys Lys Gly Lys Gly Gln Ala Leu Ile 
1105                1110                1115                1120
Pro Val Lys Glu Arg Leu Ser Ile Glu Lys Tyr Gly Gly Tyr Asn Lys 
                1125                1130                1135    
Ala Ala Gly Ala Tyr Phe Met Leu Val Glu Ser Asp Ile Glu Glu Gly 
            1140                1145                1150        
Lys Lys Gly Lys Glu Lys Thr Thr Ile Glu Phe Val Pro Ile Pro Leu 
        1155                1160                1165            
Tyr Glu Lys Ala Lys Leu Glu Ser Asp Glu Ala Arg Phe Leu Glu Glu 
    1170                1175                1180                
Arg Gly Leu Lys Glu Pro Lys Val Asn Ile Leu Ile Lys Lys Ile Lys 
1185                1190                1195                1200
Asp Thr Leu Phe Glu Val Asp Gly Phe Lys Met Trp Leu Ser Gly Arg 
                1205                1210                1215    
Thr Leu Ile Phe Lys Cys Ala Asn Gln Leu Ile Leu Asp Glu Glu Asn 
            1220                1225                1230        
Met Lys Thr Leu Lys Lys Val Val Lys Phe Ile Gln Arg Gln Lys Lys 
        1235                1240                1245            
Asn Val Lys Ile Ser Lys Lys Asp Gly Ile Thr Glu Glu Val Leu Met 
    1250                1255                1260                
Lys Tyr Glu Thr Phe Leu Asp Lys Leu Lys Asn Tyr Gly Ile Arg Leu 
1265                1270                1275                1280
Ser Thr Gln Ala Lys Thr Leu Val Asn Gly Lys Glu Lys Phe Glu Lys 
                1285                1290                1295    
Leu Ser Leu Glu Asp Lys Cys Gln Val Leu Tyr Glu Ile Leu His Leu 
            1300                1305                1310        
Phe Gln Cys Gln Ser Ser Ala Ala Asn Asn Ala Leu Lys Met Ile Gly 
        1315                1320                1325            
Gly Pro Gly Asn Ile Leu Val Asn Asn Asn Ile Ser Lys Cys Asn Lys 
    1330                1335                1340                
Ile Ser Ile Ile Asn Gln Ser Pro Thr Gly Ile Tyr Glu Lys Glu Ile 
1345                1350                1355                1360
Asp Leu Leu Lys Ile Gly Glu Asp 
                1365            

<210> 189
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #70

<400> 189
Met Lys Lys Glu Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Ser Ile Lys Lys Asn Leu Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Ser Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Asn Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Asp Val Glu Glu Leu Phe Lys Gln Phe Val Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Glu Glu Gln His Leu Ser Glu Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Arg Ala Glu Asn 
    210                 215                 220                 
Val Ile Lys Leu Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Leu Leu Lys Leu Ile Val Gly Leu Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Asn Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Arg Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Val Thr Asp Arg Gly Lys Thr Arg Glu Arg Phe Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Glu Asp Tyr Leu Lys Lys Glu Tyr Glu Glu Phe Asp 
                565                 570                 575     
Ser Ala Asp Ile Thr Gly Ile Glu Asp Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Val Ser Lys Asp Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Leu Thr Glu Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ser 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ala Ile Lys Glu Thr Glu Asn Leu 
705                 710                 715                 720 
Lys Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Arg Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Asn Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Glu Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Thr Ile Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Lys Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Arg Lys Asn Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Arg Glu Glu Asn Lys Ala Thr Ala Lys Met His Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Ser Ser Glu Glu Pro Ile Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Ile Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Leu Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Tyr Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asn Leu Leu Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile Asp Glu Ser Asn Ser Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Glu Glu His Arg Ala 
    1250                1255                1260                
Gln Phe Asp Glu Leu Leu Asp Tyr Ile Ile Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Met Gly Ala Pro Ala Ala Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Asn Ile Asp Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 190
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #71

<400> 190
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Ser Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Ser Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Asp Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Leu Phe Lys Gln Phe Val Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Leu Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Arg Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Asn Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Asn 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Arg Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Val Asp Asp Arg Gly Lys Thr Gln Glu Arg Phe Ser Ala Glu Glu 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Asp 
                565                 570                 575     
Ser Pro Thr Ile Lys Gly Ile Glu Asp Glu Phe Asn Ala Ser Phe Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Gly Val Ser Lys Glu Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ser 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ala Ile Lys Glu Thr Glu Asn Leu 
705                 710                 715                 720 
Lys Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Thr Ile Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Asp Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Val Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Asn Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala His Glu Glu Asn Lys Ala Thr Ala Lys Lys His Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ser Ser Glu Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Glu Lys Asp Ile Ala Thr Val Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Val Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Tyr Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asn Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Glu Glu His Arg Ala 
    1250                1255                1260                
Gln Phe Asp Glu Leu Leu Asp Tyr Ile Ile Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Asn Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Arg Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 191
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #72

<400> 191
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Leu Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Asn Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Asp Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Glu Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Asp 
                565                 570                 575     
Ser Pro Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Gly Val Ser Lys Glu Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ala Ile Lys Glu Thr Glu Asn Leu 
705                 710                 715                 720 
Lys Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asn Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Lys Leu Leu Arg Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Asn Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys His Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ser Ser Glu Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Glu Lys Asp Ile Ala Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Tyr Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asn Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Tyr Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Ala 
    1250                1255                1260                
Gln Phe Asp Glu Leu Leu Asp Tyr Val Ile Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Asn Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Arg Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 192
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #73

<400> 192
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Pro Met Ser Glu Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Ile Ser Glu Thr Phe Glu Gln Phe Leu Asp Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Leu Val Asp Glu Thr Ile Lys Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ile Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Gly Asn Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Ala His Leu Pro Glu Lys Tyr Tyr Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Ser Lys Thr Asp 
    370                 375                 380                 
Asn Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Leu Asp Arg Gln Ser Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Arg Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Glu Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Ile Glu Ile Phe Asp Asp Leu Phe Lys Lys Lys Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Val Asn Tyr Leu Glu Asn Glu Tyr Gln Tyr Ile Asp 
                565                 570                 575     
Ser Pro Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Gly Val Ser Lys Glu Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Ile Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Ser Phe 
    690                 695                 700                 
Lys Glu Lys Ile Glu Lys Ala Gln Ala Ile Lys Glu Thr Glu Asn Leu 
705                 710                 715                 720 
Lys Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Asp Ser Asn Leu Leu Lys Glu Lys Pro 
785                 790                 795                 800 
Leu Asp Asn Glu Gln Leu Lys Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asn Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Lys Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Lys Leu Leu Arg Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Leu Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Asn Val Asn Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Lys Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Asn Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Asn Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Met Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys His Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ser Ser Glu Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Glu Lys Asp Ile Ser Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Arg Gln Met Asn Ile Val Lys Lys Val Glu Lys Gln Lys Gly Arg 
    1090                1095                1100                
Phe Tyr Lys Glu Thr Ile His Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Ile Lys Asn Asn Leu Asp Pro Lys Lys Tyr Gly Gly Phe Asn Glu 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Pro Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Thr Ala Tyr Leu Glu Lys 
    1170                1175                1180                
Thr Gly Tyr Asn Asn Pro Lys Glu Asn Val Leu Met Glu Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Glu Leu Leu Tyr His Ala Lys Arg Tyr Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Ala 
    1250                1255                1260                
Gln Phe Asp Glu Leu Leu Asp Tyr Val Ile Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Asn Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Asn Ile Ser Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ser Thr Leu Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Arg Leu Glu Lys Leu Gly Glu Asp 
                1365            

<210> 193
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #74

<400> 193
Met Lys Lys Asn Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Lys Asp Asp Tyr Thr Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Gln Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Pro Met Ser Glu Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Ile Ser Glu Thr Phe Glu Gln Phe Leu Asp Thr Tyr 
            180                 185                 190         
Asn Glu Ile Phe Ser Glu Gln Glu Leu Val Asp Glu Ile Thr Lys Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Gln 
    210                 215                 220                 
Val Leu Ala Leu Phe Pro Asn Glu Lys Arg Asn Gly Leu Phe Gly Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Asp Asp Ile Lys Leu Gln Leu Ser Glu Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Gly Asn Ile Asp Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Asp Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Gln 
305                 310                 315                 320 
Met Ile Glu Leu Tyr Asp Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Ala His Leu Pro Glu Lys Tyr Tyr Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Lys Lys Thr Asp 
    370                 375                 380                 
Asn Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Ser Phe Tyr Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Leu Arg Ala Ile Leu Asp Arg Gln Ser Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Arg Asp Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Glu Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Ile Val Asp Leu Glu Lys Ser Ala Glu Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys Arg Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Glu Arg Gly Lys Thr Gln Glu Asn Phe Ser Ser Gln Glu 
    530                 535                 540                 
Lys Ile Glu Ile Phe Asp Asp Leu Phe Lys Lys Lys Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Val Asn Tyr Leu Glu Asn Glu Tyr Gln Tyr Ile Asp 
                565                 570                 575     
Ser Pro Asp Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Ser Lys Ile Gly Val Ser Lys Glu Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Glu Ile Leu Glu Asp Ile Ile Lys Ile Leu Thr 
    610                 615                 620                 
Ile Phe Glu Asp Arg Lys Met Arg Lys Lys Gln Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Thr Leu Ser Glu Glu Thr Leu Lys Lys Leu Glu Arg Lys His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Lys Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Glu Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Ser Phe 
    690                 695                 700                 
Lys Glu Lys Ile Glu Lys Ala Gln Ala Ile Lys Glu Thr Glu Asn Ile 
705                 710                 715                 720 
Lys Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Ile Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Lys Asn Asn Ser Lys Gln Arg Leu Lys Lys Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Asp Ser Asn His Val Lys Glu Lys Pro 
785                 790                 795                 800 
Leu Asp Asn Glu Gln Leu Lys Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asn Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Lys Glu Ile Val Arg Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Gln Leu Leu Arg Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Arg Ala His Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Leu Leu Asp Gln Arg Phe Asn Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Asn Ile Asn Ile Val Leu Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Lys Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Asn Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Asn Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Met Phe Lys Glu Asp Lys Ala Thr Ala Lys Lys His Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ser Lys Glu Glu Leu Ile Asn Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Glu Lys Asp Leu Ser Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Lys Gln Met Asn Ile Val Lys Lys Val Glu Lys Gln Lys Gly Arg 
    1090                1095                1100                
Leu Tyr Lys Glu Thr Ile His Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Ile Lys Asn Asn Leu Asp Pro Lys Lys Tyr Gly Gly Phe Asn Glu 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Pro Ile 
        1155                1160                1165            
Met Asp Arg Lys Ala Phe Glu Lys His Pro Thr Ala Tyr Leu Glu Lys 
    1170                1175                1180                
Thr Gly Tyr Asn Asn Pro Thr Glu Asn Val Leu His Glu Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Ser Arg Arg Met Ile Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Glu Leu Leu Tyr His Ala Lys Arg Tyr Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Ala 
    1250                1255                1260                
Gln Phe Asp Glu Leu Leu Asp Tyr Val Ile Asp Phe Ser Asn Lys Tyr 
1265                1270                1275                1280
Ile Asn Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asn Glu Ala Asp Ile Lys Glu Leu Val Glu Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Thr Phe Thr Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Asn Ile Ser Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Gly 
    1330                1335                1340                
Ser Thr Leu Ile Phe Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Arg Leu Glu Lys Leu Gly Glu Asp 
                1365            

<210> 194
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #75

<400> 194
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Asp Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Asn Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Asp Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Glu Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Asp 
                565                 570                 575     
Ser Pro Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Ile Gly Val Ser Lys Glu Val Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Leu Asp Glu Glu Ile Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ala Ile Thr Glu Thr Glu Asn Leu 
705                 710                 715                 720 
Lys Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Lys Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu His Gln Arg Phe Asn Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Ser Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ser Ser Glu Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Asp Ile Ala Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Tyr Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asn Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Tyr Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Ala 
    1250                1255                1260                
Gln Phe Asp Glu Leu Leu Asp His Val Ile Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Thr Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Asn Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Arg Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 195
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #76

<400> 195
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Arg Met 
            20                  25                  30          
Lys Ile His Gly Asn Thr Asp Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Lys Asp Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asn Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Asn Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Glu Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Asp Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Asn Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ser Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     
Asn Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Lys Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Tyr Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Asp Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Asn Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Glu Cys Glu Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asn Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Asp 
                565                 570                 575     
Ser Pro Thr Ile Lys Gly Ile Glu Asp Asn Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Ile Gly Val Ser Arg Glu Val Leu Asp 
        595                 600                 605             
Asp Glu Asp Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Leu Asp Glu Asp Ile Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Leu Ile Thr Glu Thr Glu Asn Leu 
705                 710                 715                 720 
Lys Asp Ile Val Gln Asn Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Gly Ser Asp Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Lys Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu His Gln Arg Phe Asn Thr Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Asp Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Ser Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Glu Phe Phe Ser Ser Glu Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Asp Ile Ala Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Ile Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Tyr Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asn Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Tyr Ile Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ala Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg His 
    1250                1255                1260                
Gln Phe Asp Glu Leu Leu Asp His Val Ile Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asn Glu Ala Asp Ile Lys Glu Leu Ala Lys Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Thr Phe Asn Lys Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Asn Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Lys Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 196
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #77

<400> 196
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Asp Leu Val Arg Lys Arg Met 
            20                  25                  30          
Lys Ile Asn Gly Asn Thr Asp Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Lys Asp Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asn Glu Ser Phe Tyr Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Lys Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Asn Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Glu Glu Thr Phe Lys Glu Phe Leu Gln Glu Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Leu Gln Pro Pro Val Asp Glu Asp Ile Asp Val 
        195                 200                 205             
Glu Ala Ile Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Asn Leu Phe Pro Thr Glu Lys Ser Asn Gly Met Phe His Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Asn Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ser Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     
Asn Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Phe Glu Ile Phe Arg 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Glu Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Ser Phe Leu Lys Lys Ile Asp Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Tyr Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Glu Arg Gln Gly Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ile Glu Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Asn Ser Ala Thr Arg Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Thr Val Phe Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Glu Cys Glu Asn Phe Ser Ser Lys Glu 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asn Leu Phe Lys Lys Asn Arg Lys Val Ser 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asn Tyr Leu Lys Asn Glu Tyr Gln Tyr Ile Asp 
                565                 570                 575     
Ser Pro Thr Ile Lys Gly Ile Glu Asn Asn Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Ile Gly Val Ser Arg Glu Val Leu Asp 
        595                 600                 605             
Asn Glu Asp Tyr Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Leu Asp Glu Asp Ile Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Thr Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ala 
        675                 680                 685             
Leu Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Lys Glu Ile Glu Lys Ala Gln Leu Lys Thr Glu Ser Glu Asn Leu 
705                 710                 715                 720 
Lys Asp Ile Val Asp Asn Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Gln Gly Lys Arg Asn Ser Lys Gln Arg Leu Lys Arg Ile 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Gly Ser Asp Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Gln Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Asn Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Ser Ile Leu His Gln Arg Phe Asn Thr Glu Asn Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Asp Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Ser Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Glu Phe Phe Ser Ser Glu Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Asp Ile Ala Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Ile Val Ile Thr His Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ile Phe Glu Lys Asn Pro Ile Ala Tyr Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr Val Asn Pro Lys Glu Asn Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Tyr Ile Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ala Glu Ser Glu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg His 
    1250                1255                1260                
Gln Phe Asp Glu Leu Leu Asp His Val Ile Asn Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Ala Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asn Glu Ala Glu Ile Lys Glu Leu Ala Lys Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Thr Phe Asn Lys Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Asn Ile Glu Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Lys Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 197
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #78

<400> 197
Met Lys Lys Gln Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Ser Asp Asp Tyr Asp Leu Ile Arg Lys Arg Met 
            20                  25                  30          
Lys Ile Asn Gly Asp His Asp Lys Gly Lys Met Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Ile 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Arg Arg Arg Arg Asn Arg Ile His 
65                  70                  75                  80  
Tyr Leu Gln Ser Ile Phe Lys Asp Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asn Glu Ser Phe Tyr Val Pro Met Asp Lys Glu 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 
Ser Asn Glu Gln Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Glu Glu Thr Phe Lys Glu Phe Leu His His Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Leu Gln Pro Pro Ile Ser Glu Asp Ile Ser Val 
        195                 200                 205             
Glu Ala Glu Leu Thr Glu Lys Val Ser Arg Ser Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Asn Lys Phe Gly Thr Glu Lys Ser Asn Gly Met Phe His Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Ser Leu Glu Glu Asp Arg Lys Leu Asn Ile Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Asp Ile Gly Glu Asp Tyr Arg Glu 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Val His Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asp Lys Thr Lys Ala Ser Leu Ser Ser Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Gln Asp Leu Ser Ala Leu Lys 
                325                 330                 335     
Asn Phe Ile Lys Ala His Leu Pro Asp Arg Tyr Phe Asp Ile Phe Arg 
            340                 345                 350         
Asn Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Glu Glu Asp Phe Tyr Lys Tyr Leu Lys Lys Val Leu Asn Lys Val Asp 
    370                 375                 380                 
Gly Ala Glu Ser Phe Leu Lys Lys Ile Asp Glu Glu Arg Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Tyr Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Glu Lys Gln Gly Gln Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Leu Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Gln Ser Lys Phe Ala Trp 
    450                 455                 460                 
Ile Glu Arg Lys Gly Asp Glu Ser Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Glu Val Asp Leu Val Ser Ser Ala Glu Arg Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Ile Gln Lys Phe Asn Val Phe Asn Glu Leu Thr Lys Val Gln 
        515                 520                 525             
Tyr Ile Asp Asp Gln Gly Lys Arg Cys Glu Asn Phe Ser Ser Lys Glu 
    530                 535                 540                 
Lys Gln Glu Ile Tyr Asp Asn Leu Phe Lys Lys Asn Arg Thr Val Ser 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asp Tyr Leu Lys Asn Glu Tyr Gln Tyr Leu Asp 
                565                 570                 575     
Asn Pro Thr Ile Lys Gly Ile Glu Asn Lys Phe Asn Ala Ser Leu Lys 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Ile Gly Val Ser Arg Glu Val Leu Asp 
        595                 600                 605             
Asn Glu Asn Tyr Glu Glu Arg Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Glu Lys Phe Ser 
625                 630                 635                 640 
Asp Val Leu Asp Gln Asp Ile Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Glu Gln Thr Gln Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Asp Ala 
        675                 680                 685             
Pro Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asp Leu Ser Phe 
    690                 695                 700                 
Lys Lys Thr Ile Glu Lys Ala Arg Leu Lys Thr Glu Ser Asp Asn Leu 
705                 710                 715                 720 
Lys Asp Ile Val Asp Lys Ile Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Glu Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Gln Gly Gln Arg Asn Ala Lys Glu Arg Leu Lys Arg Ile 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Lys Ser Asp Leu Leu Lys Lys His Pro 
785                 790                 795                 800 
Val Asp Gln Glu Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Asn Gln Glu Leu Asp Ile Asn Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Thr Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Glu Glu Val Val Lys Asn Met Lys 
865                 870                 875                 880 
Pro Phe Trp Ser Ser Leu Tyr Arg Ser Asn Leu Ile Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Ser Ile Leu His Gln Arg Tyr Asn Thr Glu Asn Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Ala Phe Asp Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Leu Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Asp Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Arg Lys Thr Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Ile Glu Arg Phe Ser Ser Glu Glu Gln Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Ser Lys Asp Ile Ala Lys Ile Lys Arg Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Thr Gly Asn 
    1090                1095                1100                
Phe Ser Lys Glu Thr Val Gln Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Ile Ile Phe Thr His Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Lys Arg Val Lys Glu Ile Ile Gly Ile Thr Ile 
        1155                1160                1165            
Met Asp Gln Ser Ile Phe Glu Ala Asp Gln Ile Lys Tyr Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr Val Asn Pro Lys Glu Asn Ile Lys Phe Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Asn Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Thr His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Cys Lys Arg Tyr Ile Asp Ser Ser Phe Asn 
        1235                1240                1245            
Ala Asp His Glu Glu Gln Lys Gln Thr Tyr Ile Asn Glu Asn Arg His 
    1250                1255                1260                
Gln Phe Asn Glu Leu Leu Asp His Val Leu Asn Phe Ser Ala Gln Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Ala Asn Glu Glu Lys Ile Lys Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asn Gln Ala Glu Val Ser Glu Leu Ala Thr Ser Phe Ile Asn Leu 
            1300                1305                1310        
Met Asp Phe Asn Lys Met Gly Ala Pro Ser Ala Phe Lys Phe Phe Gly 
        1315                1320                1325            
Thr Asn Ile Glu Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Lys 
1345                1350                1355                1360
Glu Val Gly Lys Leu Gly Glu Asp 
                1365            

<210> 198
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #79

<400> 198
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Thr Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Ile Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Ser Thr Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu Asn Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Asp Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Glu Lys Ser Ala Thr Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Glu Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Asp Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Ser Ala Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp 
        595                 600                 605             
Asp Pro Glu Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Lys Gln Leu Ser Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Leu Asp Glu Glu Ile Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ala Ile Thr Asp Thr Glu Asn Leu 
705                 710                 715                 720 
Gln Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Pro Phe Trp Glu Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr His Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ala Ser Asp Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Asn Ile Ala Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Arg Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asn Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys His Tyr Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Ala 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Asp His Val Ile Asp Phe Ala Glu Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Gln Glu Ala Asp Ile Lys Glu Leu Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Arg Leu Gly Lys Leu Gly Glu Asp 
                1365            

<210> 199
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #80

<400> 199
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Arg Leu Val Ala Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Ala Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Glu Leu Gln Glu Ile Phe Ala Glu Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      
Phe Phe Ala Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Thr Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Lys Ser Tyr 
        115                 120                 125             
His Gln Gln Tyr Pro Thr Ile Tyr His Leu Arg Gln His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Leu Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Gln Phe Ser Glu Pro Glu Pro Val Asp Glu Thr Val Asp Val 
        195                 200                 205             
Ala Val Ile Leu Thr Glu Lys Leu Ser Lys Thr Lys Lys Ala Glu Lys 
    210                 215                 220                 
Val Leu Lys Tyr Phe Pro Asn Glu Lys Ser Asn Gly Tyr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Glu Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Glu Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Gln Ala Lys Asn Val Tyr Asp Ala Ile Leu Leu Ser Glu 
    290                 295                 300                 
Ile Leu Ser Thr Thr Thr Lys Asn Thr Lys Ala Lys Leu Ser Ala Gly 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu Asn Leu Pro Glu Lys Tyr Asn Glu Phe Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Glu Leu Ser Gly Ile Asp 
    370                 375                 380                 
Gly Ser Glu Pro Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Thr Glu Leu Arg Ala Ile Ile Asp Arg Gln Lys Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Ala Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Gln Ser Pro Phe Ala Trp 
    450                 455                 460                 
Leu Ile Arg Lys Ser Glu Glu Lys Ile Lys Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Met Glu Lys Ser Ala Met Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Met Ile Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Lys Asp Glu Arg Gly Lys Thr His Glu Tyr Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Arg Glu Ile Phe Asp Glu Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Gln Asn Phe Leu Tyr Leu Asn Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Asn Ala Glu Ile Lys Gly Ile Glu Lys Ala Phe Asn Ala Ser Tyr Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Leu Ala Glu Met Gln Glu Trp Leu Glu 
        595                 600                 605             
Asp Pro Glu Met Glu Ser Met Phe Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Glu Met Ile Arg Thr Gln Leu Ser Lys Tyr Glu 
625                 630                 635                 640 
Ala Val Leu Gly Glu Glu Ile Leu Lys Lys Leu Ala Lys Lys His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Glu Lys Leu Ile Gln Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly 
        675                 680                 685             
Phe Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ala Ile Thr Asp Thr Glu Asn Leu 
705                 710                 715                 720 
Gln Glu Ile Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Glu Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Asp Phe Gly Ser Gln Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Pro Leu Asp Ile His Arg 
            820                 825                 830         
Leu Ser Asp Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Arg Asp Asp Val Pro Ser Glu Lys Val Val Lys Lys Met Arg 
865                 870                 875                 880 
Pro Phe Trp Glu Lys Leu Tyr Arg Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ile Glu Arg Gly Gly Leu Thr Glu Glu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu His Gln Arg Phe Asn Gln Lys Gln Glu 
    930                 935                 940                 
Asp Glu Gly Lys Ala Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Ile Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Ile Ala Leu Leu Lys Lys Tyr Pro Gln Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Leu Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala His Lys Glu Asn Lys Ala Thr Ala Lys Lys Glu Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Glu Ser Asp Glu Pro Ile Cys Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Phe Trp Asp Lys Ser Lys Asn Ile Ala Gln Ile Lys Lys Val Ile Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Val Gln Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Ser Lys Tyr Gly Gly Phe Gly Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Thr Val Ala Phe Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Arg Lys Leu Lys Arg Ala Lys Ala Ile Glu Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Gln Asn Pro Ile Ala Phe Leu Lys Asn 
    1170                1175                1180                
Lys Gly Tyr Glu Asn Ala Lys Glu Asn Val Phe Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Val Ala Ser 
                1205                1210                1215    
His Lys Glu Ala Gln Lys Ala Asn Ser Phe Ile Leu Pro Glu His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys His Tyr Asp Glu Ile Ser Phe His 
        1235                1240                1245            
Lys Glu Ser Phe Glu Gln Lys Gln Asp Tyr Val Asn Glu His Arg Ser 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Asp His Val Ile Asp Phe Ala Gly Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Ile Glu Lys Ile Gln Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Gln Glu Ala Asp Ile Ala Glu Leu Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Asp 
        1315                1320                1325            
Val Thr Ile Pro Arg Lys Arg Tyr Thr Ser Leu Thr Glu Ile Trp Asp 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Arg Met Gly Lys Leu Gly Glu Gln 
                1365            

<210> 200
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #81

<400> 200
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Arg Leu Val Ala Lys Lys Met 
            20                  25                  30          
Ser Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Ala Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Glu Leu Gln Glu Ile Phe Ala Glu Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      
Phe Phe Ala Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
Tyr Thr Arg Phe Pro Ile Phe Ala Thr Leu Glu Glu Glu Lys Ser Tyr 
        115                 120                 125             
His Gln Gln Tyr Pro Thr Ile Tyr His Leu Arg Gln His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Leu Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Glu Leu Asp Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Gln Phe Ser Glu Pro Glu Pro Val Asp Glu Thr Glu Gly Val 
        195                 200                 205             
Ala Val Ile Leu Thr Glu Lys Leu Ser Lys Thr Lys Lys Val Glu Lys 
    210                 215                 220                 
Val Leu Lys Tyr Tyr Pro Asn Glu Lys Ser Asn Gly Tyr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Glu Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Glu Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Gln Ala Lys Asn Val Tyr Asp Ala Ile Leu Leu Ser Glu 
    290                 295                 300                 
Ile Leu Ser Thr Thr Thr Lys Asn Thr Lys Ala Lys Leu Ser Ala Gly 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Lys Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu Asn Leu Pro Glu Lys Tyr Asn Glu Phe Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Glu Leu Ser Gly Ile Asp 
    370                 375                 380                 
Gly Ser Glu Pro Phe Leu Glu Lys Ile Asp Gln Glu Asn Phe Leu Leu 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Ala Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Thr Glu Leu Arg Ala Ile Ile Asp Arg Gln Lys Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Ala Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Gln Ser Pro Phe Ala Trp 
    450                 455                 460                 
Leu Glu Arg Lys Ser Ala Glu Lys Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Met Glu Lys Ser Ala Met Arg Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Phe Met Ile Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Lys Asp Glu Arg Gly Lys Thr His Glu Tyr Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Arg Glu Ile Phe Asp Glu Leu Phe Lys Lys His Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Gln Asn Phe Leu Tyr Leu Asn Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Asn Ala Glu Ile Lys Gly Ile Glu Lys Ala Phe Asn Ala Ser Tyr Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Leu Ala Glu Met Gln Glu Trp Leu Glu 
        595                 600                 605             
Asp Pro Glu Met Glu Ser Met Phe Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Ile Phe Glu Asp Arg Glu Met Ile Arg Thr Gln Leu Ser Lys Tyr Glu 
625                 630                 635                 640 
Ala Val Leu Gly Glu Glu Ile Leu Lys Lys Leu Ala Lys Lys His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Glu Lys Leu Ile Gln Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Asp 
        675                 680                 685             
Phe Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp His Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Leu Ala Ile Ser Asp Thr Gln Ser Leu 
705                 710                 715                 720 
Leu Glu Val Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Trp Gln Ser Leu Lys Ile Val Glu Glu Leu Val Glu Ile Ile Gly 
            740                 745                 750         
Gly Tyr Asn Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Lys Thr Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Asn Phe Gly Ser Pro Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Pro Leu Asp Ile His Arg 
            820                 825                 830         
Leu Ser Glu Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Ile Val 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Arg Asp Asp Val Pro Ser Glu Lys Val Val Lys Lys Met Arg 
865                 870                 875                 880 
Pro Phe Trp Glu Lys Leu Gln Arg Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Ala Asn Leu Thr Lys Ile Glu Arg Gly Gly Leu Thr Glu Glu Asp 
            900                 905                 910         
Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu His Gln His Phe Asn Gln Lys Gln Glu 
    930                 935                 940                 
Asp Glu Gly Lys Ala Ser Gly Lys Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Thr Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Ile Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn His His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Ile Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asn Tyr Ile Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Leu His Lys Glu Asn Lys Ala Thr Ala Lys Lys Glu Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Glu Ser Asp Glu Pro Ser Cys Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Phe Trp Asp Lys Ser Arg Asn Ile Ala Gln Ile Lys Lys Val Ile Ser 
        1075                1080                1085            
Ser His Gln Val Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Tyr Lys Glu Thr Val Asn Pro Lys Gly Asn Pro Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Ser Lys Tyr Gly Gly Phe Gly Ser 
                1125                1130                1135    
Pro Val Val Ala Tyr Thr Val Ala Phe Ile Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Arg Lys Leu Lys Arg Ala Lys Ala Ile Glu Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Gln Ser Ala Phe Glu Gln Asp Pro Ile Ala Phe Leu Lys Asn 
    1170                1175                1180                
Lys Gly Tyr Glu Asn Val Lys Glu Asn Glu Phe Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Val Ala Ser 
                1205                1210                1215    
His Lys Glu Ala Gln Lys Ala Asn Ser Phe Ile Leu Pro Glu His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys His Tyr Asp Glu Ile Ser Phe His 
        1235                1240                1245            
Lys Glu Ser Phe Glu Gln Lys Gln Asp Tyr Val Asn Glu His Arg Ser 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Asp His Val Ile Asp Phe Ala Gly Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Ile Glu Lys Ile Gln Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Gln Glu Ala Asp Ile Ala Glu Leu Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Asp 
        1315                1320                1325            
Val Thr Ile Pro Arg Lys Arg Tyr Thr Ser Leu Thr Glu Ile Trp Asp 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Arg Met Gly Asn Leu Gly Glu Gln 
                1365            

<210> 201
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #82

<400> 201
Met Thr Lys Asp Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Arg Leu Met Ala Lys Lys Met 
            20                  25                  30          
Ser Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Ala Arg Leu Phe Asp Glu Gly Gln Thr Ala Glu Ala Arg Arg Ser 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Leu Ala Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Glu Leu Gln Glu Ile Phe Ala Glu Glu Ile Leu Lys Ile Asp Glu His 
                85                  90                  95      
Phe Phe Val Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Gln 
            100                 105                 110         
Tyr Thr Arg Phe Pro Ile Phe Pro Thr Leu Glu Glu Glu Lys Ser Tyr 
        115                 120                 125             
Tyr Gln Gln Tyr Pro Thr Ile Tyr His Leu Arg Gln Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Ile Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Leu Lys Tyr Arg Gly His Phe Leu Phe Glu Gly Glu Leu Asp Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Glu Val Tyr 
            180                 185                 190         
Asn Gln Gln Phe Asp Gln Pro Glu Ala Val Asp Glu Thr Glu Gly Val 
        195                 200                 205             
Ala Val Ile Leu Thr Glu Lys Leu Ser Lys Thr Lys Lys Val Glu Lys 
    210                 215                 220                 
Ile Leu Lys Tyr Tyr Pro Thr Glu Lys Thr Asn Gly Tyr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Glu Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ser Leu Leu Glu Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Gln Ala Lys Lys Val Tyr Asp Ala Ile Leu Leu Ser Glu 
    290                 295                 300                 
Ile Leu Ser Thr Thr Thr Lys Asn Thr Lys Ala Lys Leu Ser Ala Gly 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Ala Lys Leu Lys 
                325                 330                 335     
Gln Phe Val Lys Glu Asn Leu Pro Glu Lys Thr Thr Val Phe Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Phe Val Lys Lys Glu Leu Ser Gly Ile Val 
    370                 375                 380                 
Gly Ser Glu Pro Phe Leu Glu Lys Ile Asp Gln Glu Asn Phe Leu Leu 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Ala Asn Gly Val Ile Pro His Gln Val His Leu 
                405                 410                 415     
Thr Glu Leu Lys Ala Ile Ile Asp Arg Gln Lys Gln Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Glu Glu Ala Arg Glu Lys Ile Ile Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Gln Ser Ser Phe Ala Trp 
    450                 455                 460                 
Leu Glu Arg Lys Thr Ala Glu Lys Ile Thr Pro Trp Asn Phe Thr Glu 
465                 470                 475                 480 
Val Val Asp Met Glu Lys Ser Ala Met Arg Phe Ile Gln Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Thr Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Tyr Met Ile Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Lys Asp Glu Arg Gly Ile Lys His Glu Tyr Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Arg Glu Ile Phe Lys Glu Leu Phe Gln Lys Gln Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Lys Leu Gln Asn Phe Leu Tyr Ala Asn Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Asn Ala Glu Ile Leu Gly Ile Glu Lys Ala Phe Asn Ala Ser Tyr Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Leu Ala Lys Met Gln Glu Trp Leu Glu 
        595                 600                 605             
Gln Pro Glu Met Glu Ser Met Phe Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Ile Phe Glu Asp Arg Glu Met Ile Arg Thr Gln Leu Ser Lys Tyr Gln 
625                 630                 635                 640 
Ala Val Leu Gly Glu Glu Ile Leu Lys Lys Leu Ala Lys Lys His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Glu Lys Leu Ile His Gly Ile Arg Asp 
            660                 665                 670         
Lys Lys Thr Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Asp 
        675                 680                 685             
Phe Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp His Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Leu Ala Leu Ser Asp Asn Gln Ser Leu 
705                 710                 715                 720 
Leu Glu Val Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Trp Gln Ser Leu Lys Ile Val Glu Glu Leu Ile Glu Ile Ile Gly 
            740                 745                 750         
Gly Tyr Asn Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Lys Thr Asn Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Asn Phe Gly Ser Pro Leu Leu Lys Glu Gln Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Lys Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Pro Leu Asp Ile Asp Arg 
            820                 825                 830         
Leu Ser Glu Tyr Asp Ile Asp His Ile Ile Pro Arg Ser Phe Ile Val 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Lys Val Leu Val Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Arg Asp Asp Val Pro Ser Glu Lys Val Val Lys Lys Met Arg 
865                 870                 875                 880 
Pro Phe Trp Glu Lys Leu Gln Arg Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Ala Tyr Leu Thr Lys Ile Glu Arg Gly Gly Leu Thr Pro Glu Asp 
            900                 905                 910         
Lys Ala Arg Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu His Gln His Phe Asn Gln Lys Gln Glu 
    930                 935                 940                 
Asp Glu Gly Lys Ala Ser Gly Lys Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Thr Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Ile Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn His His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Ile Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asn Tyr Ile Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Leu Ala Lys Glu Asn Lys Ala Thr Ala Lys Lys Glu Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Leu Lys Phe Phe Glu Ser Asp Glu Pro Ser Cys Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Phe Trp Asp Lys Ser Arg His Ile Pro Gln Ile Lys Lys Val Ile Ser 
        1075                1080                1085            
Ser His Gln Val Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Tyr Lys Glu Thr Val Asn Pro Lys Gly Lys Pro Asp Lys Leu Ile 
1105                1110                1115                1120
Gln Arg Lys Asn Asn Trp Asp Val Ser Lys Tyr Gly Gly Phe Gly Ser 
                1125                1130                1135    
Pro Val Val Ala Tyr Ala Val Ala Phe Ile Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Arg Lys Leu Lys Lys Ala Lys Ala Ile Glu Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Gln Ser Ala Phe Glu Gln Asp Pro Ile Ala Phe Leu Lys Asn 
    1170                1175                1180                
Lys Gly Tyr Ser Asn Val Thr Glu Asn Glu Phe Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Val Ala Ser 
                1205                1210                1215    
His Lys Glu Ala Gln Lys Ala Asn Ser Phe Ile Leu Pro Glu Gln Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Gln His Tyr Asp Glu Ile Ser Phe His 
        1235                1240                1245            
Lys Glu Ser Phe Glu Gln Lys Gln Asp Tyr Val Asn Glu His Leu Ser 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Asp Gln Val Ile Asp Phe Ala Gly Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Ile Glu Lys Ile Gln Glu Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Gln Glu Ala Asp Thr Ala Glu Leu Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Asp 
        1315                1320                1325            
Val Thr Ile Pro Arg Lys Arg Tyr Thr Ser Leu Thr Glu Ile Trp Asp 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Arg Met Gly Asn Leu Gly Glu Gln 
                1365            

<210> 202
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #83

<400> 202
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Thr Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Glu Asn Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile His Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Leu Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Glu Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Gln Gln Ile Phe Asp Asp Leu Phe Lys Gln Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Ser Ala Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp 
        595                 600                 605             
Asp Pro Glu Asn Glu Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Leu Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ala Ser Thr Asn Thr Glu Asp Leu 
705                 710                 715                 720 
Gln Glu Ile Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Glu Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Ala Phe Trp Glu Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala His Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Asn Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr His Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ala Ser Asp Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Asn Ile Ala Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Arg Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asn Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys His Cys Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Ala 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Asp His Val Ile Asp Phe Ala Glu Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Arg Leu Gly Asp Leu Gly Glu Asp 
                1365            

<210> 203
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #84

<400> 203
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Glu Asp Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Thr Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Phe Thr Glu Lys Val Ser Arg Ala Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Thr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Thr Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Glu Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Ala Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Lys Asn Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Ser Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile His Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Leu Ala Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Met Leu Tyr Glu Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Glu Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Gln Gln Ile Phe Asp Asp Leu Phe Lys Gln Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Ser Ala Thr Ile Lys Gly Ile Glu Asn Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp 
        595                 600                 605             
Asp Pro Glu Asn Glu Glu Met Leu Glu Glu Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Leu Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Glu Lys Ala Gln Ala Glu Thr Asn Thr Glu Asp Leu 
705                 710                 715                 720 
His Glu Ile Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Arg Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Glu Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Ala Phe Trp Glu Lys Leu Tyr Arg Ser Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala His Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr His Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ala Ser Asp Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Asn Ile Ala Thr Val Lys Lys Val Met Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ser Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Arg Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Glu Phe Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asn Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys His Cys Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Ala 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Asp His Val Lys Asp Phe Ala Glu Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Leu Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Asn Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Arg Leu Gly Asp Leu Gly Glu Asp 
                1365            

<210> 204
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #85

<400> 204
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Glu Asn Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Glu Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Glu Glu Thr Phe Lys Thr Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ser Glu Gln Glu Pro Val Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Leu Phe Thr Glu Lys Val Ser Arg Ala Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Thr Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Thr Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Glu Tyr Glu 
            260                 265                 270         
Glu Glu Leu Glu Ala Leu Leu Ala Ile Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Ala Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Lys Glu Leu Pro Glu Lys Tyr Asn Glu Ile Phe Arg 
            340                 345                 350         
Asp Ser Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Ser Val Ser 
        355                 360                 365             
Gln Ala Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Lys Ile Pro 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile His Lys Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asp Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Leu Ala Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Met Leu Tyr Glu Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Glu Asn Phe Ser Ser Glu Glu 
    530                 535                 540                 
Lys Lys Gln Ile Phe Asp Glu Leu Phe Lys Gln Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Leu Leu Glu Asn Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Ser Ala Thr Ile Lys Gly Ile Glu Asn Ser Phe Asn Ala Ser Tyr Gly 
            580                 585                 590         
Thr Tyr His Asp Phe Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp 
        595                 600                 605             
Asp Pro Glu Asn Glu Glu Met Leu Glu Glu Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Tyr Ser 
625                 630                 635                 640 
Asp Val Phe Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Gln Ala Glu Thr Asn Thr Glu Asp Leu 
705                 710                 715                 720 
His Glu Ile Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Arg Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Glu Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu Tyr Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Ile 
865                 870                 875                 880 
Ala Phe Trp Glu Lys Leu Lys Arg Ser Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Leu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala His Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Thr Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr His Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ala Ser Asp Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Asn Ile Ala Thr Val Lys Lys Val Met Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Gln Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ser Arg Lys Thr Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Arg Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Glu Phe Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asn Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys His Cys Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Gly Lys Ser Leu Glu Gln Lys Gln Glu Tyr Val Asn Glu His Arg Ala 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Asp Tyr Val Lys Asp Phe Ala Glu Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Leu Glu Lys Ile Leu Lys Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Asn Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Arg Leu Gly Asp Leu Gly Glu Asp 
                1365            

<210> 205
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #86

<400> 205
Met Asn Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Asp Glu Asn Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Ile Gln Gly Asn Gly Asp Lys Lys Lys Val Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Ala Gly Glu Thr Ala Glu Gly Thr Arg Met 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Gly Glu Met Asp Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Glu Lys Arg 
            100                 105                 110         
Asn Glu Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Ser Ser Val Lys Glu Thr Phe Lys Thr Phe Leu Gln Ala Tyr 
            180                 185                 190         
Asn Gln Ala Phe Thr Val Gln Glu Pro Val Asn Glu Thr Ile Asp Val 
        195                 200                 205             
Gly Ser Leu Phe Gln Glu Arg Val Ser Arg Gln Arg Lys Ala Glu Asn 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Thr Phe Met Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Gly Asn Phe Lys Lys Thr Phe 
                245                 250                 255     
Gly Leu Glu Glu Asp Ala Lys Leu Gln Phe Ser Lys Glu Glu Tyr Glu 
            260                 265                 270         
Glu Glu Leu Glu Gly Leu Leu Ala Ile Val Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Ala Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Lys Asp Ile Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Leu Ile Lys Arg Tyr Glu Glu His Lys Thr Asp Leu Glu Gln Leu Lys 
                325                 330                 335     
Lys Phe Val Arg Lys Glu Leu Pro Glu Lys Tyr Tyr Glu Val Phe Arg 
            340                 345                 350         
Asp Ser Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Ser Val Ser 
        355                 360                 365             
Gln Ala Asp Phe Tyr Lys Tyr Leu Lys Lys Ile Leu Glu Lys Ile Pro 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Arg Ala Ile Ile Arg Lys Gln Ser Arg Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asp Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Thr Asp Gly Ser Ile Arg Pro Trp Asn Phe Thr Glu 
465                 470                 475                 480 
Val Val Asp Glu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Ser Tyr Leu Pro Gln Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Met Leu Tyr Glu Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Glu Thr Gln Glu Asn Phe Ser Ser Val Glu 
    530                 535                 540                 
Lys Lys Glu Ile Phe Asp Glu Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Tyr Ala Leu Leu Glu Asn Phe Leu Lys Asn Gln Tyr Met Tyr Ile Glu 
                565                 570                 575     
Thr Ala Glu Ile Lys Gly Ile Glu Lys Ser Phe Asn Ala Ser Tyr Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Gly Val Ser Gln Glu Met Leu Asp 
        595                 600                 605             
Asp Pro Glu Asn Glu Glu Met Phe Glu Glu Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Lys Glu Gln Leu Ser Lys Tyr Ala 
625                 630                 635                 640 
Asp Tyr Phe Glu Ala Gly Val Leu Lys Lys Leu Val Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Lys Lys Thr Ile Leu Asp Tyr Leu Met Arg Asp Asp Asp 
        675                 680                 685             
Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Glu Ser Ile Ala Gln Gln Gln Ala Glu Asp Gln Thr Glu Asp Leu 
705                 710                 715                 720 
His Glu Ile Val Lys Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Ile Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Pro Pro Thr Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Arg Lys Lys Ser Lys Pro Arg Gln Lys Val Leu 
    770                 775                 780                 
Glu Glu Ala Met Lys Glu Phe Gly Ser Asn Leu Leu Lys Glu Tyr Pro 
785                 790                 795                 800 
Pro Asp Asn Ala Asn Leu Arg Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Lys Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Met Val Leu Val Ser Ser Thr Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Ile 
865                 870                 875                 880 
Gly Phe Trp Glu Lys Leu Lys Arg Ser Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Leu Asn Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala His Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu His Gln Lys Tyr Asn Ser Glu Asp Glu 
    930                 935                 940                 
Asp Glu Gly Asn Thr Ile Arg Thr Thr Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Gln Ile Tyr Gln Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Arg Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Gln Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Leu Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Asn Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Arg Phe Phe Ala Ser Asp Gln Pro Lys Val Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Gln Ser Lys Asn Ile Ala Met Val Lys Lys Val Met Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Thr Glu Ile Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Gln Pro Lys Gly Asp Ser Ala Lys Leu Ile 
1105                1110                1115                1120
Ala Arg Lys Thr Gly Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Ile Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Arg Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Arg Gln Phe Glu Lys Asn Glu Val Glu Phe Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Arg Glu Asn Val Leu Val Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Leu Glu Asn Gly Arg Arg Arg Leu Ile Ala Ser 
                1205                1210                1215    
Ala Thr Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Asn His Leu 
            1220                1225                1230        
Val Glu Leu Leu Tyr His Ala Lys His Cys Asp Asp Leu Asn Phe Asp 
        1235                1240                1245            
Gly Lys Ser Leu Glu Gln Lys Gln Ala Tyr Leu Asn Ala His Arg Ala 
    1250                1255                1260                
Glu Phe Asn Glu Leu Phe Glu Tyr Val Lys His Phe Ala Glu Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Lys Asn Leu Lys Lys Leu Ser Lys Leu Tyr Glu Gln 
                1285                1290                1295    
Asn Gln Glu Ala Asp Ile Lys Val Met Ala Gln Ser Phe Leu Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Asp Phe Lys Phe Phe Asp 
        1315                1320                1325            
Thr Thr Ile Pro Arg Lys Arg Tyr Thr Ser Ile Lys Glu Ile Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Asn Gln Ser Ile Thr Gly Leu Tyr Glu Thr His Trp 
1345                1350                1355                1360
Lys Leu Gly Glu Leu Gly Glu Asp 
                1365            

<210> 206
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #87

<400> 206
Met Lys Lys Glu Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Asp Tyr Asp Leu Val Lys Lys Lys Met 
            20                  25                  30          
Lys Val His Gly Asn Thr Glu Lys Lys Lys Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Ile Thr Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Gln Glu Glu Met Asn Lys Val Asp Glu Asn 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Ala Thr Leu Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Lys Leu Asn Thr 
                165                 170                 175     
Glu Asn Thr Ser Val Glu Glu Thr Phe Lys Gln Phe Leu Gln Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Ala Asn Gly Glu Pro Leu Asp Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Val Ser Arg Thr Arg Lys Ala Glu Lys 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Lys Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Glu Glu Asp Ala Lys Ile Gln Phe Ala Lys Glu Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Leu Ala Ala Lys Asn Val Tyr Asp Ala Val Glu Leu Ser Ser 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Asn Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Glu Glu His Lys Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Glu Asn Leu Pro Glu Lys Tyr Asn Glu Ile Phe Lys 
            340                 345                 350         
Asp Ser Gln Lys Asp Gly Tyr Ala Gly Tyr Ile Glu Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Lys Ile Leu Glu Asn Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Glu Lys Ile Glu Gln Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Val Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu Gln Ala Ile Ile His Arg Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Ser Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Pro Ile Arg Pro Trp Asn Leu Glu Glu 
465                 470                 475                 480 
Val Val Asp Leu Gly Lys Ser Ala Thr Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Gln Lys Tyr Met Val Phe Asn Glu Leu Thr Lys Val Ser 
        515                 520                 525             
Tyr Ile Asp Asp Arg Gly Lys Thr Gln Glu Asn Phe Ser Gly Glu Glu 
    530                 535                 540                 
Lys Gln Gln Ile Phe Asp Asp Leu Phe Lys Gln Lys Arg Lys Val Lys 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Gln Phe Leu Arg Asn Glu Tyr Asn Tyr Ile Glu 
                565                 570                 575     
Ser Ala Thr Ile Lys Gly Ile Glu Asp Ser Phe Asn Ala Ser Phe Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Gly Val Thr Gln Glu Val Leu Asp 
        595                 600                 605             
Asp Pro Glu Asn Ala Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Arg Lys Met Ile Arg Glu Gln Leu Ser Lys Phe Ser 
625                 630                 635                 640 
Asp Val Leu Asp Glu Glu Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe 
    690                 695                 700                 
Lys Asn Glu Ile Glu Lys Ala Gln Ala Ser Thr Asn Asp Glu Asp Leu 
705                 710                 715                 720 
Gln Glu Ile Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Glu Ile Met Gly 
            740                 745                 750         
Gly Tyr Pro Pro Lys Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ser Arg Gly Lys Arg Asn Ser Lys Pro Arg Leu Lys Ala Leu 
    770                 775                 780                 
Glu Lys Ala Met Lys Glu Leu Gly Ser Asn Leu Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Ala Leu Arg Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Thr Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Lys Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Ala Phe Trp Glu Lys Leu Tyr Arg Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala His Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Ser Glu Lys Asp 
    930                 935                 940                 
Glu Glu Gly Asn Thr Ile Arg Lys Val Arg Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Thr Ser Gln Phe Arg Lys Thr Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Thr Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr His Lys Phe Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Ala Phe Lys Glu Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Thr 
1025                1030                1035                1040
Asn Ile Met Lys Phe Phe Ala Lys Asp Glu Pro Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Tyr Leu Ala Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr His Gln Met Asn Ile Val Lys Lys Val Glu Ile Gln Lys Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Thr Ile Lys Pro Lys Gly Asn Ser Asn Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Val Ala Tyr Ser Val Val Ile Thr Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Arg Lys Leu Lys Arg Val Lys Glu Ile Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Thr Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Pro Lys Glu Asn Val Leu Met Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys His Cys Asp Glu Ser Asn Phe Asn 
        1235                1240                1245            
Ser Glu Ser Leu Glu Gln Lys Gln Ala Tyr Val Glu Glu His Arg Ala 
    1250                1255                1260                
Glu Phe Ser Glu Ile Leu Asp His Val Ile Asp Phe Ala Glu Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Ala Asn Leu Glu Lys Ile Lys Lys Leu Phe Glu Glu 
                1285                1290                1295    
Asn Gln Glu Ala Asp Ile Lys Glu Ile Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Gln Phe Asn Ala Met Gly Ala Pro Ala Ser Phe Lys Phe Phe Glu 
        1315                1320                1325            
Lys Thr Ile Glu Arg Lys Arg Tyr Thr Ser Ile Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Arg 
1345                1350                1355                1360
Arg Leu Gly Asp Leu Gly Glu Asp 
                1365            

<210> 207
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #88

<400> 207
Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          
Lys Val Ala Gly Asn Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  
Asn Arg Thr Thr Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Val Asp Ala Asn 
                85                  90                  95      
Phe Phe Tyr Arg Leu Asp Asp Ser Phe Tyr Val Glu Ser Glu Lys Arg 
            100                 105                 110         
His Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Lys Leu Val Asn 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Lys Leu Asp Thr 
                165                 170                 175     
Lys Asn Thr Ser Val Asp Glu Val Phe Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         
Asn Gln Val Phe Ala Ser Gly Ile Arg Leu Glu Glu Asn Lys Asp Val 
        195                 200                 205             
Ala Lys Ile Leu Ser Glu Lys Val Thr Arg Lys Glu Lys Leu Glu Lys 
    210                 215                 220                 
Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser Thr Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Ile Ser Leu Ile Val Gly Ser Lys Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Asp Glu Lys Thr Asp Ile Glu Cys Ala Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Glu 
        275                 280                 285             
Ile Phe Val Ala Ala Lys Asn Ala Tyr Asn Ala Val Val Leu Ser Ser 
    290                 295                 300                 
Ile Ile Thr Val Thr Asp Thr Glu Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Asp Lys His Glu Lys Asp Leu Lys Gln Leu Lys 
                325                 330                 335     
Ala Phe Ile Lys Val His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Asn 
            340                 345                 350         
Asp Val Glu Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Lys 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Met Lys Lys Met Leu Glu Asn Ile Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Ile Asp Lys Ile Glu Glu Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile Pro His Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Glu Ala Ile Leu His Gln Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Tyr Glu Lys Ile Lys Ser Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly Gln Ser Glu Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Lys Val Asp Phe Gly Lys Ser Ala Ile Asp Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Gln Gly Lys Thr His Glu Tyr Phe Ser Gly Gln Glu 
    530                 535                 540                 
Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Lys Arg Lys Val Lys 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Gln Phe Leu Arg Asn Met Ser Asn His Ile Glu 
                565                 570                 575     
Ser Pro Thr Ile Glu Gly Val Glu Asp Ser Phe Asn Ser Ser Phe Ala 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Val Gly Val Thr Gln Glu Ile Leu Asp 
        595                 600                 605             
Asn Pro Leu Asn Ala Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu Gln Gln Phe Ser 
625                 630                 635                 640 
Asp Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Ile Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe 
    690                 695                 700                 
Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Ala Asp Lys Asp Ile 
705                 710                 715                 720 
Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Gly Ile Met Gly 
            740                 745                 750         
Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gly Lys Gly Lys Asn Asn Ser Lys Pro Arg Phe Lys Ser Leu 
    770                 775                 780                 
Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Gly Leu Arg Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Ala Asn Arg 
    850                 855                 860                 
Glu Lys Gly Asp Asp Val Pro Ser Leu Glu Val Val Arg Lys Arg Lys 
865                 870                 875                 880 
Val Phe Trp Glu Lys Leu Tyr Gln Ala Asn Leu Met Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Cys Glu Lys Asp 
    930                 935                 940                 
Glu Asn Gly Asn Thr Ile Glu Gln Val Arg Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Asn Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr His Gln Phe Asp Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Trp Phe Lys Ala Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Thr 
1025                1030                1035                1040
Asn Ile Met Leu Phe Phe Ala Lys Lys Asp Arg Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Tyr Leu Asp Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Arg Gln Met Asn Ile Val Lys Lys Thr Glu Ile Gln Lys Gly Glu 
    1090                1095                1100                
Phe Ser Lys Ala Thr Ile Lys Pro Lys Gly Asn Ser Ser Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ala Asn Trp Asp Pro Met Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Asn Met Ala Tyr Ala Val Val Ile Glu Tyr Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Ile Lys Glu Lys Glu Ile Ile Arg Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Thr Ala Phe Glu Lys Asp Gln Lys Ala Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Arg Asn Pro Lys Glu Asn Val Leu Thr Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Cys Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Asn Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Asn His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Asn Cys Glu Ala Ser Asn Phe Asp 
        1235                1240                1245            
Gly Lys Ser Leu Glu Gln Lys Gln Ala Tyr Ile Glu Ser His Arg Glu 
    1250                1255                1260                
Met Phe Ala Glu Leu Leu Ala His Val Ser Glu Phe Ala Lys Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Ala Asn Leu Glu Lys Ile Asn Gln Leu Phe Glu Gln 
                1285                1290                1295    
Asn Lys Glu Gly Asp Ile Lys Val Ile Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Ala Phe Asn Ala Met Gly Ala Pro Ala Ser Phe Lys Phe Phe Glu 
        1315                1320                1325            
Thr Thr Ile Glu Arg Lys Arg Tyr Asn Asn Leu Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Ala Arg Lys 
1345                1350                1355                1360
Arg Leu Asp Asp Leu Gly Glu Asp 
                1365            

<210> 208
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #89

<400> 208
Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          
Lys Val Ala Gly Asn Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  
Asn Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ser Lys Ile Asp Ala Asn 
                85                  90                  95      
Phe Phe Cys Arg Leu Asn Asp Ser Phe Tyr Val Asp Ser Glu Lys Arg 
            100                 105                 110         
Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
                165                 170                 175     
Lys Asn Thr Ser Val Asp Gly Val Tyr Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         
Asn Gln Val Phe Ala Ser Gly Ile Lys Leu Glu Glu Asn Lys Asp Val 
        195                 200                 205             
Ala Lys Ile Leu Ala Glu Lys Val Thr Arg Lys Glu Lys Leu Glu Arg 
    210                 215                 220                 
Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser Thr Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Ile Ser Leu Ile Val Gly Ser Lys Gly Asn Phe Gln Lys Val Phe 
                245                 250                 255     
Asp Leu Val Glu Lys Thr Asp Ile Glu Cys Ala Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Ile Ile Gly Asp Glu Tyr Ala Glu 
        275                 280                 285             
Leu Phe Val Ala Ala Lys Asn Ala Tyr Asn Ala Val Val Leu Ser Ser 
    290                 295                 300                 
Ile Ile Thr Val Thr Asp Thr Glu Thr Asn Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Asp Thr His Glu Lys Asp Leu Ser Glu Leu Lys 
                325                 330                 335     
Ala Phe Ile Lys Leu His Leu Pro Lys Gln Tyr Glu Glu Ile Phe Ser 
            340                 345                 350         
Asn Val Glu Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Lys 
        355                 360                 365             
Gln Ala Asp Phe Tyr Lys Tyr Met Lys Thr Met Leu Glu Asn Ile Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Ile Ala Lys Ile Glu Glu Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile Pro His Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Glu Ala Ile Leu His Gln Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Tyr Asp Lys Ile Lys Ser Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly Gln Ser Glu Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Lys Val Asp Phe Gly Lys Ser Ala Val Asp Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Tyr Ile Asp Asp Gln Gly Lys Thr Asn Glu Tyr Phe Ser Gly Gln Glu 
    530                 535                 540                 
Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Lys Arg Lys Val Lys 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Gln Phe Leu Arg Asn Met Ser His His Ile Glu 
                565                 570                 575     
Ser Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn Ser Ser Tyr Ala 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Val Gly Ile Lys Gln Glu Ile Leu Asp 
        595                 600                 605             
Asn Pro Leu Asn Thr Glu Met Leu Glu Asp Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu Gln Gln Phe Ser 
625                 630                 635                 640 
Asp Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Ile Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe 
    690                 695                 700                 
Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Ala Asp Lys Asp Ile 
705                 710                 715                 720 
Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Ser Ile Met Gly 
            740                 745                 750         
Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gly Lys Gly Lys Asn Asn Ser Arg Pro Arg Tyr Lys Ser Leu 
    770                 775                 780                 
Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Glu Leu Arg Asn Asn Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala Gly Asn Arg 
    850                 855                 860                 
Glu Lys Gly Asp Asp Val Pro Pro Leu Glu Ile Val Arg Lys Arg Lys 
865                 870                 875                 880 
Val Phe Trp Glu Lys Leu Tyr Gln Gly Asn Leu Met Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Tyr Glu Lys Asp 
    930                 935                 940                 
Asp His Gly Asn Thr Met Glu Gln Val Arg Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Asn Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr His Gln Phe Asp Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Trp Phe Lys Ala Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Thr 
1025                1030                1035                1040
Asn Ile Met Leu Phe Phe Ala Gln Lys Asp Arg Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Tyr Leu Asp Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Arg Gln Met Asn Ile Val Lys Lys Thr Glu Ile Gln Lys Gly Glu 
    1090                1095                1100                
Phe Ser Lys Ala Thr Ile Lys Pro Lys Gly Asn Ser Ser Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ala Asn Trp Asp Pro Met Lys Tyr Gly Gly Leu Asp Ser 
                1125                1130                1135    
Pro Asn Met Ala Tyr Ala Val Val Ile Glu Tyr Asp Ile Ala Lys Gly 
            1140                1145                1150        
Lys Lys Lys Lys Leu Val Phe Glu Lys Lys Ile Ile Arg Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Lys Ala Phe Glu Lys Asp Glu Lys Ala Phe Leu Glu Glu 
    1170                1175                1180                
Gln Gly Tyr Arg Gln Pro Lys Glu Asn Val Leu Thr Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Cys Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Asn Glu Ala Gln Lys Gly Asn Gln Gln Val Leu Pro Asn His Leu 
            1220                1225                1230        
Val Thr Leu Leu His His Ala Lys Asn Cys Glu Ala Ser Asn Phe Asp 
        1235                1240                1245            
Gly Lys Ser Leu Glu Gln Lys Gln Asp Tyr Ile Glu Ser Asn Arg Glu 
    1250                1255                1260                
Met Phe Ala Glu Leu Leu Ala His Val Ser Glu Phe Ala Lys Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Ala Asn Leu Asn Lys Ile Asn Gln Leu Phe Glu Gln 
                1285                1290                1295    
Asn Lys Glu Gly Asp Ile Lys Val Ile Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Ala Phe Asn Ala Met Gly Ala Pro Ala Ser Phe Lys Phe Phe Glu 
        1315                1320                1325            
Thr Thr Ile Glu Arg Lys Arg Tyr Asn Asn Leu Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Ala Arg Lys 
1345                1350                1355                1360
Arg Leu Asp Asp Leu Gly Glu Asp 
                1365            

<210> 209
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #90

<400> 209
Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          
Lys Val Ala Gly Asn Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Glu Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  
Asn Arg Thr Thr Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Ala Glu Met Ser Glu Val Asp Ala Asn 
                85                  90                  95      
Phe Phe Tyr Arg Leu Glu Asp Ser Phe Tyr Ile Glu Ser Glu Lys Arg 
            100                 105                 110         
His Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Arg Thr Ile Tyr His Leu Arg Glu Lys Leu Val Asn 
    130                 135                 140                 
Ser Ser Asp Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Lys Leu Asp Thr 
                165                 170                 175     
Lys Asn Thr Ser Val Asp Glu Val Phe Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         
Asn Gln Val Phe Ala Ser Asp Ile Arg Leu Glu Glu Asn Lys Glu Val 
        195                 200                 205             
Ala Lys Ile Leu Ser Glu Lys Leu Thr Arg Arg Glu Lys Leu Asp Lys 
    210                 215                 220                 
Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser Thr Gly Met Phe Ala Gln 
225                 230                 235                 240 
Phe Ile Ser Leu Ile Ile Gly Ser Lys Gly Asn Phe Lys Lys Val Phe 
                245                 250                 255     
Asp Leu Asp Glu Lys Thr Asp Ile Glu Cys Ala Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Ala Leu Leu Ala Lys Ile Gly Asp Glu Tyr Ala Glu 
        275                 280                 285             
Ile Phe Val Ala Ala Lys Asn Ala Tyr Asn Ala Val Val Leu Ser Ser 
    290                 295                 300                 
Ile Ile Thr Val Thr Asp Thr Glu Thr Lys Ala Lys Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Phe Asp Lys His Glu Lys Asp Leu Lys Arg Leu Lys 
                325                 330                 335     
Ala Phe Phe Lys Val His Leu Pro Glu Lys Phe Asn Glu Val Phe Asn 
            340                 345                 350         
Asp Val Glu Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Lys 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Met Lys Lys Met Leu Glu Asn Ile Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Ile Asp Gln Ile Glu Glu Glu Asn Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile Pro His Gln Leu His Leu 
                405                 410                 415     
Glu Glu Leu Glu Ala Ile Leu His Gln Gln Ala Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asp Tyr Glu Lys Ile Lys Ser Leu Val Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly Gln Ser Glu Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg Pro Trp Asn Ile Glu Glu 
465                 470                 475                 480 
Lys Val Asp Phe Gly Lys Ser Ala Ile Asp Phe Ile Glu Lys Met Thr 
                485                 490                 495     
Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Cys Tyr Gln Lys Tyr Met Val Tyr Asn Glu Leu Thr Lys Ile Arg 
        515                 520                 525             
Tyr Ile Asp Asp Gln Gly Lys Thr His Glu Tyr Phe Ser Gly Gln Glu 
    530                 535                 540                 
Lys Gln Gln Ile Phe Asn Asp Leu Phe Lys Gln Lys Arg Lys Val Lys 
545                 550                 555                 560 
Lys Lys Asp Leu Glu Gln Phe Leu Tyr Asn Met Met Asn His Ile Glu 
                565                 570                 575     
Ser Pro Thr Ile Glu Gly Val Glu Asp Ala Phe Asn Ser Ser Phe Ala 
            580                 585                 590         
Thr Tyr His Asp Leu Gln Lys Val Gly Val Thr Gln Glu Ile Leu Asp 
        595                 600                 605             
Asn Pro Leu Asn Ala Glu Met Leu Glu Glu Ile Val Lys Ile Leu Thr 
    610                 615                 620                 
Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu Gln Gln Phe Ser 
625                 630                 635                 640 
Asp Val Leu Asp Gly Ala Val Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Ile Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met Asn Asp Asp Gly 
        675                 680                 685             
Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser Asn Leu Ser Phe 
    690                 695                 700                 
Lys Ser Ile Ile Glu Lys Glu Gln Val Ser Thr Ala Asp Lys Asp Ile 
705                 710                 715                 720 
Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Gly Ile Met Gly 
            740                 745                 750         
Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gly Lys Gly Lys Asn Asn Ser Lys Pro Arg Phe Thr Ser Leu 
    770                 775                 780                 
Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Gly Leu Lys Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile His Asn 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser Phe Ile Thr 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Ala Asn Arg 
    850                 855                 860                 
Glu Lys Gly Asp Asn Val Pro Ser Leu Glu Val Val Arg Lys Arg Lys 
865                 870                 875                 880 
Val Phe Trp Glu Lys Leu Tyr Gln Ala Lys Leu Met Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Cys Lys Lys Asp 
    930                 935                 940                 
Glu Asn Gly Asn Thr Ile Glu Gln Val Arg Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Gly Val 
            980                 985                 990         
Val Ala Asn Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr His Gln Phe Asp Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Trp Phe Lys Ala Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Thr 
1025                1030                1035                1040
Asn Ile Met Leu Phe Phe Ala Lys Lys Asp Arg Ile Ile Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Asn Gly Glu Ile 
            1060                1065                1070        
Leu Trp Asp Lys Ser Lys Tyr Leu Asp Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Arg Gln Met Asn Ile Val Lys Lys Thr Glu Ile Gln Lys Gly Glu 
    1090                1095                1100                
Phe Ser Asn Ala Thr Val Asn Pro Lys Gly Asn Ser Ser Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ala Asp Trp Asp Pro Ile Lys Tyr Gly Gly Phe Asp Gly 
                1125                1130                1135    
Ser Asn Met Ala Tyr Ala Ile Val Ile Glu Tyr Asp Ile Glu Lys Arg 
            1140                1145                1150        
Lys Lys Lys Thr Val Ile Lys Glu Lys Glu Leu Ile Gln Ile Asn Ile 
        1155                1160                1165            
Met Glu Arg Thr Ala Phe Glu Lys Asp Gln Lys Ala Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Arg Asn Pro Lys Glu Asn Val Leu Thr Lys Ile Pro Lys 
1185                1190                1195                1200
Tyr Thr Leu Tyr Glu Cys Glu Asn Gly Arg Arg Arg Met Leu Gly Ser 
                1205                1210                1215    
Ala Asn Glu Ala Gln Lys Gly Asn Gln Met Val Leu Pro Asn His Leu 
            1220                1225                1230        
Met Thr Leu Leu Tyr His Ala Lys Asn Cys Glu Ala Ser Asn Phe Asp 
        1235                1240                1245            
Gly Lys Ser Leu Glu Gln Lys Gln Ala Tyr Ile Glu Ser His Arg Glu 
    1250                1255                1260                
Met Phe Ala Glu Leu Leu Ala His Ile Ser Glu Phe Ala Lys Arg Tyr 
1265                1270                1275                1280
Thr Leu Ala Asp Ala Asn Leu Glu Lys Ile Asn Thr Leu Phe Glu Gln 
                1285                1290                1295    
Asn Lys Glu Gly Asp Ile Lys Val Ile Ala Gln Ser Phe Val Asn Leu 
            1300                1305                1310        
Met Ala Phe Asn Ala Met Gly Ala Pro Ala Ser Phe Lys Phe Phe Glu 
        1315                1320                1325            
Thr Thr Ile Glu Arg Lys Arg Tyr Asn Asn Leu Lys Glu Leu Leu Asn 
    1330                1335                1340                
Ala Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Ala Arg Lys 
1345                1350                1355                1360
Arg Leu Asp Asp Leu Gly Glu Asp 
                1365            

<210> 210
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #91

<400> 210
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Tyr Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Gln Phe Val Glu Val Tyr 
            180                 185                 190         
Asn Gln Thr Phe Glu Glu Ser His Leu Ser Glu Glu Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Ala Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu His Leu Pro Glu Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ala Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Arg Gly Lys Thr Arg Glu Phe Phe Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Lys Asp Tyr Leu Lys Lys Glu Phe Glu Glu Phe Asp 
                565                 570                 575     
Ser Val Asp Ile Thr Gly Ile Glu Asp Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu 
705                 710                 715                 720 
Lys Glu Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Glu Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Lys Tyr Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Asp Arg Glu Arg Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Glu Arg Pro Leu Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Ile Gln Glu Asn Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile Asp Lys Ser Asn Gly Asn 
        1235                1240                1245            
Ser Glu Asn Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Asp Glu Leu Leu Asp Tyr Ile Ile Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 211
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #92

<400> 211
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Pro Glu Asp Lys Lys 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe His Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asp Pro Leu Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Gln Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Ser Asp Glu Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Glu Phe Leu Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asn Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Asp Ile Thr Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala 
625                 630                 635                 640 
Asp Leu Phe Asp Lys Asn Val Leu Lys Lys Leu Lys Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Gln Thr Asp Ser Leu 
705                 710                 715                 720 
Lys Glu Val Val Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Gln Gly Ile Lys Asn Ser Arg Gln Arg Met Lys Arg Leu 
    770                 775                 780                 
Glu Glu Val Leu Lys Lys Leu Gly Ser Asn Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Thr Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Lys Val Leu Thr Ser Ser Glu Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Tyr Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ser Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Lys Tyr Asp Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Asp Lys Glu Arg Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asn Gly Thr 
                1045                1050                1055    
Ile Ile Lys Arg Pro Leu Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Gln Asp Ile Gln Glu Asn Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Phe Leu Tyr His Ala Ser Arg Ile Asp Lys Ser Thr Gly Ser 
        1235                1240                1245            
Ser Glu Asn Leu Glu Gln Lys Gln Glu Tyr Val Glu Gln His Arg His 
    1250                1255                1260                
Glu Phe Asp Glu Ile Leu Asp Tyr Ile Ile Asp Phe Ser Glu Arg Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Asn Leu Tyr Asn Glu 
                1285                1290                1295    
Asn Asp Asp Ser Asp Ile Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Ala Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 212
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #93

<400> 212
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe His Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asp Pro Leu Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Gln Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Asn Asp Glu Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Glu Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asn Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Glu Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Glu Phe Leu Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Asp Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Lys Arg Leu Ala Thr Tyr Ala 
625                 630                 635                 640 
Asp Leu Phe Asp Lys Asn Val Leu Lys Gln Leu Lys Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ser Gly Gln Thr Asp Ser Leu 
705                 710                 715                 720 
Asn Glu Gln Ile Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Gln Gly Ile Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 
Glu Glu Val Leu Lys Lys Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Thr Gln Leu Gln Asn Glu Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Glu Leu Asp Ile Asn Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Glu Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Ser Tyr Trp Gln Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ser Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Pro Ile Arg Asn Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Lys Tyr Asp Leu Arg Lys Met Ile Ala Lys 
    1010                1015                1020                
Ser Asp Lys Glu Arg Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asn Gly Thr 
                1045                1050                1055    
Ile Ile Lys Arg Pro Leu Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Gln Asp Ile Gln Glu Asn Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Thr Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Phe Leu Tyr His Ala Ser Lys Ile Asp Lys Ser Thr Gly Ser 
        1235                1240                1245            
Ser Glu Asn Leu Glu Gln Lys Gln Glu Tyr Val Glu Gln His Arg His 
    1250                1255                1260                
Glu Phe Asp Glu Ile Leu Asp Tyr Ile Ile Asp Phe Ser Glu Arg Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Asn Leu Phe Asn Glu 
                1285                1290                1295    
Asn Asp Asp Ser Asp Ile Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe Lys Phe Leu Asp 
        1315                1320                1325            
Ala Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 213
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #94

<400> 213
Met Glu Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Ser Asp Val Asp Lys Leu Phe Tyr Gln Leu Val Gln Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Leu Asp Ala Ser Gly Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Ala Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Glu Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Glu Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Glu Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 
His Glu Gln Ile Ala Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Lys Gly Leu Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         
Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ser Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Pro Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys 
    1010                1015                1020                
Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Arg Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asn Gly Glu 
                1045                1050                1055    
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Ser Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Lys Asp Ile Gln Lys Asp Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Thr Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Phe Leu Tyr His Ala Ser Lys Ile Glu Lys Ser Thr Gly Ser 
        1235                1240                1245            
Ser Glu Asn Leu Glu Gln Lys Gln Ala Tyr Val Glu Gln His Arg His 
    1250                1255                1260                
Glu Phe Asp Glu Ile Leu Glu Tyr Ile Ile Asp Phe Ser Glu Arg Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Ser Lys Val Lys Ser Leu Phe Asn Glu 
                1285                1290                1295    
His Glu Asp Ser Asp Ile Ser Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe Lys Phe Leu Asp 
        1315                1320                1325            
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 214
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #95

<400> 214
Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 
His Glu Gln Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         
Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 
Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys 
    1010                1015                1020                
Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Arg Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu 
                1045                1050                1055    
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Ser Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Lys Asp Val Gln Lys Asp Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Gly Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala Lys Leu 
            1220                1225                1230        
Val Thr Phe Leu Tyr His Ala Ser Lys Ile Glu Lys Ser Lys Gly Ser 
        1235                1240                1245            
Pro Glu Asn Asn Glu Gln Lys Gln Ala Tyr Val Glu Gln His Arg His 
    1250                1255                1260                
Asp Leu Asp Glu Ile Leu Glu Tyr Ile Ser Glu Phe Ser Lys Arg Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Ser Lys Val Lys Ser Leu Phe Asn Lys 
                1285                1290                1295    
His Glu Asp Ser Ser Ile Ser Glu Leu Ala Ser Ser Ile Ile Asn Leu 
            1300                1305                1310        
Phe Thr Leu Thr Ser Leu Gly Ala Pro Ala Ala Phe Lys Phe Leu Asp 
        1315                1320                1325            
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 215
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #96

<400> 215
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Glu Glu Ser Phe Leu Val Pro Glu Asp Lys Glu 
            100                 105                 110         
His Asp Arg His Pro Ile Phe Gly Asn Leu Ala Asp Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Ser Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe His Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Leu Leu Phe Glu Glu Asp Pro Leu Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Gln Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gly Leu Ser Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Ser Asp Glu Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Glu Glu Leu His Ala Ile Leu Arg Arg Gln Glu Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Lys Arg Lys Ser Asp Glu Ala Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Glu Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Glu Phe Leu Ser Ala Glu Gln 
    530                 535                 540                 
Lys Gln Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asn Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Asp Ile Thr Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Gln Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Lys Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala 
625                 630                 635                 640 
Asp Leu Phe Asp Lys Ser Val Leu Lys Lys Leu Lys Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Ser Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Lys Thr Glu Ser Leu 
705                 710                 715                 720 
Lys Glu Val Val Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ala Gln Gly Ile Lys Asn Ser Arg Gln Arg Met Lys Lys Leu 
    770                 775                 780                 
Glu Glu Val Leu Lys Lys Leu Gly Ser Asn Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Thr Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Asp Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Lys Val Leu Thr Ser Gln Glu Ala Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Tyr Trp Gln Lys Leu Leu Lys Ala Gly Ala Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ser Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Thr Glu Leu Asp 
    930                 935                 940                 
Glu Asn Asn Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gly Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Lys Tyr Asp Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Asp Lys Glu Arg Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asn Gly Thr 
                1045                1050                1055    
Ile Ile Lys Arg Pro Leu Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Ile Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Gln Asp Ile Gln Glu Asn Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Leu Val Leu Pro Ala His Tyr 
            1220                1225                1230        
Val Thr Phe Leu Tyr Leu Ala Ser Arg Tyr Asp Lys Leu Thr Gly Ser 
        1235                1240                1245            
Ser Glu Asp Leu Glu Gln Lys Lys His Phe Val Glu Gln His Leu His 
    1250                1255                1260                
Tyr Phe Asp Glu Ile Leu Asp Ile Ile Ile Asp Phe Ser Glu Arg Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Ser Asn Leu Glu Lys Ile Lys Asn Leu Tyr Asn Glu 
                1285                1290                1295    
Asn Asp Asp Phe Ser Ile Asn Glu Gln Ala Ser Asn Met Ile Asn Leu 
            1300                1305                1310        
Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Ala Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 216
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #97

<400> 216
Met Arg Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Asn Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ala 
                85                  90                  95      
Phe Phe Gln Arg Leu Ser Glu Ser Phe Leu Val Pro Glu Asp Lys Glu 
            100                 105                 110         
Phe Asp Arg His Pro Ile Phe Gly Asn Leu Lys Asp Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Asp Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Leu Gln Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Leu Leu Phe Glu Glu Asp Pro Ile Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Thr Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ser Glu Ile Pro Gly Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gly Leu Ser Glu Asp Ala Lys Leu Gln Ile Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Ser Asp Glu Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Lys Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Ser 
            340                 345                 350         
Asn Lys Glu Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Glu Phe Leu Ala Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Lys Glu Leu His Ala Ile Leu Arg Arg Gln Glu Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Leu Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Lys Gly Asn Ser Ser Phe Ala Trp 
    450                 455                 460                 
Leu Lys Arg Arg Ser Asp Glu Ser Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Glu Glu Ala Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Pro Leu Tyr Glu Met Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Thr Lys Pro Glu Phe Leu Ser Ala Glu Gln 
    530                 535                 540                 
Lys Glu Ala Ile Val Asp Leu Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asn Tyr Phe Ser Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Asp Ile Thr Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Ala Phe Leu Asp 
        595                 600                 605             
Asp Glu Ala Asn Gln Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Glu Lys Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala 
625                 630                 635                 640 
Asp Leu Phe Asp Lys Ser Val Leu Lys Lys Leu Lys Lys Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ala Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Ser Leu Asp Phe 
    690                 695                 700                 
Glu Lys Ile Ile Gln Asn Ala Gln Glu Lys Ala Lys Ser Glu Ser Leu 
705                 710                 715                 720 
Lys Glu Ala Val Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ala Gln Gly Ile Lys Asn Ser Arg Gln Arg Leu Lys Lys Leu 
    770                 775                 780                 
Glu Glu Val His Lys Lys Thr Gly Ser Lys Ile Leu Lys Glu His Ser 
785                 790                 795                 800 
Val Ser Asn Thr Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Leu Leu 
                805                 810                 815     
Gln Asp Gly Lys Asp Met Tyr Thr Gly Gln Asp Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Ile Val Leu Thr Ser Gln Glu Ala Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Tyr Trp Gln Lys Gln Leu Lys Ser Gly Ala Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp His Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Ser Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Ser Lys Leu Asp 
    930                 935                 940                 
Glu Asn Asn Lys Ser Asn Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Met Val Ser Asp Phe Arg Lys Asp Phe Gly Leu Tyr Lys Leu Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Ala Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys His Tyr Asp Leu Ala Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Asp Ser Ser Leu Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Lys Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Lys Arg Pro Leu Ile Glu Thr Asn Thr Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Val Lys Asp Phe Lys Thr Ile Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Ile Ala Tyr Ser Val Leu Val Val Ala Lys Val Ala Lys Gly 
            1140                1145                1150        
Lys Ala Gln Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Gln Ser Glu Phe Glu Lys Asp Pro Ile Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asp Ile Gln Glu Asn Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ala Lys Tyr 
            1220                1225                1230        
Val Lys Phe Leu Tyr Leu Ala Ser Arg Tyr Asp Lys Leu Thr Gly Lys 
        1235                1240                1245            
Ser Glu Asp Arg Glu Gln Lys Lys His Phe Val Glu Gln His Leu His 
    1250                1255                1260                
Tyr Phe Asp Glu Ile Leu Asp Ile Ile Val Asp Phe Ser Asn Arg Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Ser Asn Leu Glu Lys Ile Lys Asn Leu Tyr Asn Glu 
                1285                1290                1295    
Lys Asp Asp Phe Ser Ile Asn Glu Gln Ala Ser Asn Met Leu Asn Leu 
            1300                1305                1310        
Phe Thr Phe Thr Ser Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Ala Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Asn Glu Val Leu Asn 
    1330                1335                1340                
Ser Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Gly Asp 
                1365            

<210> 217
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #98

<400> 217
Met Arg Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg Gln Thr Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Lys 
65                  70                  75                  80  
Tyr Leu Gln Asn Ile Phe Ser Asn Glu Met Ser Lys Val Asp Ser Ala 
                85                  90                  95      
Phe Phe Gln Arg Leu Ser Glu Ser Phe Leu Val Pro Asp Asp Lys Glu 
            100                 105                 110         
Phe Asp Arg His Pro Ile Phe Gly Asn Leu Lys Asp Glu Val Ala Tyr 
        115                 120                 125             
His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Asp Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asp Ser 
                165                 170                 175     
Glu Asn Thr Asp Val His Ala Leu Phe Leu Lys Leu Val Asp Thr Tyr 
            180                 185                 190         
Asn Leu Leu Phe Glu Glu Asp Lys Ile Asp Thr Glu Thr Ile Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Thr Ala Lys Thr Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Glu Ile Pro Asn Gln Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ser Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Glu Leu Ser Glu Asp Ala Lys Leu Gln Ile Ser Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Ile Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Thr Val Lys Gly Ala Asn Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Val Gln Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Lys Leu Val Lys Glu Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Asp 
            340                 345                 350         
Asn Lys Glu Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Ser Lys Leu Asp 
    370                 375                 380                 
Gly Ala Glu Lys Leu Ile Ser Lys Leu Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Asn Glu Leu Lys Ala Ile Ile Arg Arg Gln Glu Lys Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Gln Glu Lys Ile Glu Lys Leu Phe Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Asn Ser Ser Phe Ala Trp 
    450                 455                 460                 
Leu Lys Arg Arg Ser Asn Glu Ser Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Gln Glu Ala Ser Ala Arg Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Pro Leu Tyr Glu Met Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Gln Thr Glu Gly Met Lys Lys Pro Glu Phe Leu Ser Ser Glu Glu 
    530                 535                 540                 
Lys Glu Glu Ile Val Asn Leu Leu Phe Lys Lys Glu Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asn Tyr Phe Ser Lys Ile Lys Cys Phe Asp 
                565                 570                 575     
Ser Val Thr Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Ala Phe Leu Asp 
        595                 600                 605             
Asp Glu Ala Asn Gln Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Glu Lys Met Ile Glu Lys Arg Leu Ala Lys Tyr Ala 
625                 630                 635                 640 
Asp Val Phe Asp Lys Ser Val Leu Lys Lys Leu Lys Lys Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Gln Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Thr Gly Lys Thr Ile Leu Asp Phe Leu Lys Asp Asp Gly Val 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Ser Leu Asp Phe 
    690                 695                 700                 
Ala Lys Ile Ile Lys Asn Ala Gln Glu Lys Thr Lys Ser Glu Ser Leu 
705                 710                 715                 720 
Glu Glu Thr Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Ile Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Ser Thr Ala Gln Gly Ile Lys Asn Ser Arg Gln Arg Leu Arg Lys Leu 
    770                 775                 780                 
Glu Glu Val His Lys Asn Thr Gly Ser Lys Ile Leu Lys Glu His Asn 
785                 790                 795                 800 
Val Ser Asn Thr Gln Leu Gln Ser Asp Arg Leu Tyr Leu Tyr Leu Leu 
                805                 810                 815     
Gln Asp Gly Lys Asp Met Tyr Thr Gly Lys Asp Leu Asp Phe Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Ile Val Leu Thr Ser Gln Glu Ser Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Ile Val Asn Lys Met Lys 
865                 870                 875                 880 
Ser Tyr Trp Gln Lys Gln Leu Lys Ser Gly Ala Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp His Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Phe Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Ser Asn Leu Asp 
    930                 935                 940                 
Glu Asn Ser Lys Ser Asn Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Met Val Ser Asp Phe Arg Lys Asp Phe Gly Phe Tyr Lys Leu Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Ala Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys His Tyr Asp Leu Ala Lys Leu Ile Val Lys 
    1010                1015                1020                
Ser Asp Ser Ser Leu Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Lys Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Ile Ile Thr Arg Pro Gln Ile Glu Thr Asn Thr Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Val Lys Asp Ile Lys Thr Ile Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Asn Asn Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Ile Ile Ala Tyr Ser Val Leu Val Val Ala Lys Val Ala Lys Gly 
            1140                1145                1150        
Lys Ala Gln Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Gln Asn Glu Phe Glu Lys Asp Pro Ile Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asp Ile Gln Glu Ser Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Asn Lys Tyr 
            1220                1225                1230        
Val Lys Phe Leu Tyr Leu Ala Ser Arg Tyr Thr Lys Phe Thr Gly Lys 
        1235                1240                1245            
Glu Glu Asp Arg Glu Lys Lys Arg His Phe Val Glu Ser His Leu His 
    1250                1255                1260                
Tyr Phe Asp Glu Ile Met Asp Ile Ile Val Asp Phe Ser Asn Arg Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Ser Asn Leu Glu Lys Ile Gln Asn Leu Tyr Asn Glu 
                1285                1290                1295    
Lys Asp Asn Phe Ser Ile Glu Glu Gln Ala Thr Asn Met Leu Asn Leu 
            1300                1305                1310        
Phe Thr Phe Thr Gly Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asn 
        1315                1320                1325            
Ala Asp Ile Asp Arg Lys Arg Tyr Thr Ser Thr Asn Glu Ile Leu Asn 
    1330                1335                1340                
Ser Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Gly Asp 
                1365            

<210> 218
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #99

<400> 218
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Asp Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Gln Thr Phe Glu Glu Ser His Leu Ser Glu Gln Thr Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Glu Asn Leu Pro Asp Lys Tyr Asn Glu Ile Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Lys Thr Arg Glu Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Leu Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Leu Asp Tyr Leu Lys Lys Glu Phe Glu Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu 
705                 710                 715                 720 
Lys Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Asp Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Lys Leu Leu Asn Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Lys Tyr Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Met Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Ile Gln Glu Asn Lys Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile Asp Lys Ser Asn Gly Asn 
        1235                1240                1245            
Pro Glu His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 219
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #100

<400> 219
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Glu Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Ile Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Gln Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Glu Ser His Leu Ser Glu Ile Thr Ile Asp Ala 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ala Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asn Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Glu Asn Val Pro Asp Gln Tyr Asn Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Thr Tyr Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Ser Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ala Lys Glu Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Glu Lys Leu Leu Asn Tyr Leu Lys Lys Glu Phe Glu Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp 
        595                 600                 605             
Asp Glu Ala Asn Glu Asp Ile Ile Glu Asp Ile Ile Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Val Asp Asn Leu 
705                 710                 715                 720 
Ala Gln Val Val His Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Gln Asp Ser Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Lys Lys Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Ala Arg Lys 
865                 870                 875                 880 
Ser Phe Trp Val Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Val Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Lys Lys Tyr Asn Leu Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Gly Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Met Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Gln Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr His Asn Ile Gln Glu Asp Lys Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile Asp Lys Ser Asn Gly Asn 
        1235                1240                1245            
Pro Glu His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Val Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ala Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asp Asp Ala Asp Ile Glu Glu Ile Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Gly 
        1315                1320                1325            
Glu Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Cys Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 220
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #101

<400> 220
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Val Asp Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Ile Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Gln Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Glu Glu Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Val Asn Ala Pro Asp Gln Tyr Asn Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ala Lys Glu Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp 
        595                 600                 605             
Asp Lys Ala Asn Glu Gln Ile Ile Glu Asp Ile Ile Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Gln Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Asp Val Asp Asp Ile 
705                 710                 715                 720 
Ala Gln Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Gln Asp Ser Leu Lys Asn Leu Gly Ser Asn Ile Asn Lys Lys Lys Pro 
785                 790                 795                 800 
Val Glu Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Ala Arg Lys 
865                 870                 875                 880 
Ser Phe Trp Val Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Val Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Lys Lys Tyr Asn Val Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Asp Ile Glu Thr Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asn Lys Gln Lys Asp Phe Glu Ile Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr His Asn Ile Gln Glu Asp Lys Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile Asp Lys Ser Asn Gly Asn 
        1235                1240                1245            
Pro Glu His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Val Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ala Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asp Asp Ala Ser Ile Glu Glu Ile Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Asn Phe Phe Gly 
        1315                1320                1325            
Glu Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Cys Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 221
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #102

<400> 221
Met Asn Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Ser Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Asp Glu Asp Lys Arg 
            100                 105                 110         
Gly Glu Arg His Pro Ile Phe Gly Asn Ile Ala Ala Glu Val Lys Tyr 
        115                 120                 125             
His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Thr Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Lys Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Ala Leu Phe Lys Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Lys Thr Ile Glu Glu Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Leu Ser Ile Leu Thr Glu Lys Val Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Gln Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Gly Leu Leu Gly Glu Val Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Ala Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Val Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Lys Lys Leu Lys 
                325                 330                 335     
Asp Phe Ile Lys Val Asn Ala Pro Asp Gln Tyr Asn Ala Ile Phe Lys 
            340                 345                 350         
Asp Lys Asn Lys Lys Gly Tyr Ala Gly Tyr Ile Glu Ser Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Leu Lys Ile Asn 
    370                 375                 380                 
Gly Ser Gly Asp Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Gly Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Lys Ala Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Ile Leu Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Glu Ala Lys Glu Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Asp Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg 
                565                 570                 575     
Ile Val Asn Leu Thr Gly Leu Asp Lys Glu Phe Asn Ser Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Arg Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp 
        595                 600                 605             
Asp Lys Ala Asn Glu Gln Ile Ile Glu Asp Ile Ile Gln Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Gln Arg Leu Gln Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Ala Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Asn Lys Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Tyr 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Gln Ile Ile Gly Asp Val Asp Asp Ile 
705                 710                 715                 720 
Ala Asn Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Ala Asn Ile Ile Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asp Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Leu Leu 
    770                 775                 780                 
Gln Asp Ser Leu Lys Asn Leu Asp Asn Pro Val Asn Ile Lys Asn Pro 
785                 790                 795                 800 
Val Glu Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asn Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Ala Arg Lys 
865                 870                 875                 880 
Ser Phe Trp Val Lys Leu Leu Lys Ala Gly Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu His Asp 
    930                 935                 940                 
Glu Asn Lys Lys Val Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Lys Lys Tyr Asn Val Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Lys Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Asp Ile Glu Thr Asn Lys Glu Asp Gly Glu Ile 
            1060                1065                1070        
Ala Trp Asn Lys Gln Lys Asp Phe Lys Ile Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ser Tyr Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ser Lys Lys Leu Lys Thr Val Gln Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Arg Asn Ile Arg Glu Asp Lys Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Leu Val Ile Pro Gln Arg Phe 
            1220                1225                1230        
Thr Thr Leu Leu Tyr His Ala Lys Arg Ile Asp Lys Ser Asn Gly Asn 
        1235                1240                1245            
Pro Glu His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Arg Asp 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Val Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ala Leu Phe Ala Glu 
                1285                1290                1295    
Asn Asp Asp Ala Ser Ile Glu Glu Ile Ala Thr Ser Phe Ile Ser Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Asn Phe Phe Gly 
        1315                1320                1325            
Glu Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Cys Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 222
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #103

<400> 222
Met Lys Lys Ser Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Val Asn Lys Ser Asp Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Arg Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ser Ser Glu Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Glu Glu Phe Arg 
                565                 570                 575     
Ile Asn Asp Leu Ile Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp 
        595                 600                 605             
Asp Lys Thr Asn Glu Gln Ile Ile Glu Asp Ile Ile Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Gln Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Gln Ile Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp Ile 
705                 710                 715                 720 
Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly Asp Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Arg Ser Lys Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Gln Asp Ser Leu Lys Glu Leu Gly Ser Asn Leu Asn Lys Lys Lys Pro 
785                 790                 795                 800 
Val Glu Asn Ser His Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp His 
            820                 825                 830         
Leu Ser Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Ile Glu Ile Val Arg Ala Arg Lys 
865                 870                 875                 880 
Ser Tyr Trp Val Lys Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Lys Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Lys Lys Tyr Asp Val Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser His Ser Glu Met Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Thr Glu Val Lys Tyr Ala Asp Gly Lys 
                1045                1050                1055    
Val Ile Glu Arg Pro Asp Ile Glu Thr Asn Lys Asp Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asn Lys Gln Lys Asp Phe Glu Ile Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Val Tyr Trp Asp Thr Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Ser Ile 
        1155                1160                1165            
Met Glu Arg Ser Phe Phe Glu Glu Asn Pro Val Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr His Asn Val Gln Glu Asp Lys Leu Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Phe Glu Gly Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Thr Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Glu Leu Leu Tyr His Ala His Arg Ile Asp Asn Phe Asn Gly Ser 
        1235                1240                1245            
Thr Glu His Leu Glu Gln Lys Gln Lys Tyr Val Ser Glu His Lys Lys 
    1250                1255                1260                
Glu Phe Glu Glu Val Leu Ser Cys Val Glu Asn Phe Ala Asn Leu Tyr 
1265                1270                1275                1280
Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala Ala Ala Glu Ser 
                1285                1290                1295    
Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe Asn Phe Leu Gly 
        1315                1320                1325            
Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys Glu Cys Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Glu 
                1365            

<210> 223
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #104

<400> 223
Met Glu Lys Ser Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Lys Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Glu Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Pro Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys Gln Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Val Asn Lys Leu Asp Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Ser Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ser Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Ala Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Lys Tyr His Ser Asp Glu Pro Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Ser Phe Glu Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Ala Lys Leu Leu Ser Tyr Leu Asn Asn Glu Phe Glu Glu Phe Arg 
                565                 570                 575     
Ile Asn Asp Leu Ile Gly Leu Asp Lys Asp Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp 
        595                 600                 605             
Asp Lys Thr Asn Glu Gln Ile Ile Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Ser Gln Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Asn Asn Lys Thr Ile Leu Asp Phe Leu Ile Asp Asp Gly His 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ser Leu Ser Phe 
    690                 695                 700                 
Lys Thr Ile Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp Ile 
705                 710                 715                 720 
Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly Asp Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gly Tyr Gly Arg Asn Lys Ser Asn Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Gln Asp Ser Leu Lys Glu Phe Gly Ser Asp Leu Ser Lys Lys Lys Pro 
785                 790                 795                 800 
Val Glu Asn Ser His Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Ile 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp His 
            820                 825                 830         
Leu Ser Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Ile Glu Ile Val Arg Asn Arg Lys 
865                 870                 875                 880 
Ser Tyr Trp Tyr Lys Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Lys Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Lys Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Lys Lys Tyr Asp Val Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser His Ser Glu Met Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Thr Glu Val Lys Tyr Ala Asp Gly Arg 
                1045                1050                1055    
Val Phe Glu Arg Pro Asp Ile Glu Thr Asn Lys Ala Asp Gly Glu Val 
            1060                1065                1070        
Val Trp Asn Lys Gln Lys Asp Phe Asp Ile Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Val Tyr Trp Asp Thr Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Ser Ile 
        1155                1160                1165            
Met Glu Arg Ser Phe Phe Glu Glu Asn Pro Val Ser Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr His Asn Val Gln Glu Asp Lys Leu Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Phe Glu Gly Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Thr Glu Leu Gln Lys Gly Asn Glu Val Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Glu Leu Leu Tyr His Ala His Arg Ile Asp Asn Phe Asn Gly Ser 
        1235                1240                1245            
Thr Glu His Leu Glu Gln Lys Gln Lys Tyr Val Ser Glu His Lys Lys 
    1250                1255                1260                
Glu Phe Glu Glu Val Leu Ser Cys Val Glu Asn Phe Ala Asn Leu Tyr 
1265                1270                1275                1280
Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala Ala Ala Glu Ser 
                1285                1290                1295    
Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Ala Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe Asn Phe Leu Gly 
        1315                1320                1325            
Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys Glu Cys Leu Ser 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Val Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Glu 
                1365            

<210> 224
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #105

<400> 224
Thr Lys Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys His Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Ala Asn Lys Ser Glu Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Gly Ile Leu Ser Lys Ile Asn 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Arg Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Lys 
465                 470                 475                 480 
Val Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Asp Ser Glu Phe Phe Asp Ser Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu Tyr Arg 
                565                 570                 575     
Ile Lys Asp Leu Ile Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ser Phe Leu Asp 
        595                 600                 605             
Asp Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile Lys Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Gln Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Gln Ile Ile Gln Glu Ala Gln Val Val Gly Asp Val Asp Asp Ile 
705                 710                 715                 720 
Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly Asp Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu Lys Lys Leu 
    770                 775                 780                 
Gln Asn Ser Leu Lys Glu Leu Gly Ser Asn Leu Asn Glu Glu Lys Pro 
785                 790                 795                 800 
Val Glu Asn Ser His Leu Gln Asn Asp Gln Leu Phe Leu Tyr Tyr Ile 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Asp Glu Leu Asp Ile Asp His 
            820                 825                 830         
Leu Ser Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Leu Asp Ile Val Arg Ala Arg Lys 
865                 870                 875                 880 
Ala Glu Trp Val Arg Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Lys Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Lys Lys Tyr Asp Val Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser His Ser Glu Met Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Thr Val Val Lys Tyr Ala Asp Gly Lys 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Asp Thr Gly Glu Ile 
            1060                1065                1070        
Ala Trp Asn Lys Gln Thr Asp Phe Glu Lys Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Thr Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Val Tyr Trp Asp Thr Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Ser Ile 
        1155                1160                1165            
Met Glu Arg Ser Phe Phe Glu Glu Asn Pro Val Glu Phe Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr His Asn Val Gln Glu Asp Lys Leu Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Phe Glu Gly Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Thr Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Glu Leu Leu Tyr His Ala His Arg Ile Asp Asn Phe Asn Gly Ser 
        1235                1240                1245            
Thr Glu His Leu Glu Gln Lys Gln Lys Tyr Val Ser Glu His Lys Lys 
    1250                1255                1260                
Glu Phe Glu Glu Val Leu Ser Cys Val Glu Asn Phe Ala Asn Leu Tyr 
1265                1270                1275                1280
Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala Ala Ala Asp Ser 
                1285                1290                1295    
Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Asn Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe Asn Phe Leu Gly 
        1315                1320                1325            
Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys Glu Cys Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Glu 
                1365            

<210> 225
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #106

<400> 225
Thr Lys Lys Asn Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ala Glu Glu Met Ala Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Glu Ser Phe Leu Thr Asp Asp Asp Lys Thr 
            100                 105                 110         
Phe Asp Ser His Pro Ile Phe Gly Asn Lys Ala Glu Glu Asp Ala Tyr 
        115                 120                 125             
His Gln Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Ile Phe Ala Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Arg Thr Phe Asp Asp Ser His Leu Ser Glu Ile Thr Val Asp Ala 
        195                 200                 205             
Ala Ser Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Lys Tyr Tyr Pro Thr Glu Lys Lys Asn Thr Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ala Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Lys Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Glu Leu Leu Gly Lys Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Thr Ser Ala Lys Asn Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asp Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Val Glu His His Glu Asp Leu Glu Lys Leu Lys 
                325                 330                 335     
Glu Phe Ile Lys Ala Asn Lys Ser Glu Leu Tyr His Asp Ile Phe Lys 
            340                 345                 350         
Asp Lys Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Glu Asn Gly Val Lys 
        355                 360                 365             
Gln Asp Glu Phe Tyr Lys Tyr Leu Lys Asn Ile Leu Ser Lys Ile Asp 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Asp Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Lys Asp Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Glu Tyr Arg Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Lys 
465                 470                 475                 480 
Val Ile Asp Lys Glu Lys Ser Ala Glu Lys Phe Ile Thr Arg Met Thr 
                485                 490                 495     
Leu Asn Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
His Val Tyr Glu Thr Tyr Ala Val Tyr Asn Glu Leu Thr Lys Ile Lys 
        515                 520                 525             
Tyr Val Asn Glu Gln Gly Lys Asp Ser Glu Phe Phe Asp Ser Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Val Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Glu Lys Leu Leu Asn Tyr Leu Asn Lys Glu Phe Pro Glu Tyr Arg 
                565                 570                 575     
Ile Lys Asp Leu Ile Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Lys Lys Ile Leu Lys Asp Lys Ala Phe Leu Asp 
        595                 600                 605             
Asp Lys Val Asn Glu Glu Val Ile Glu Asp Ile Ile Lys Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Lys Asp Met Ile His Glu Arg Leu Gln Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Ala Asn Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Tyr Lys Leu Ile Asn Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Asn Asn Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Thr Leu Pro Phe 
    690                 695                 700                 
Lys Gln Ile Ile Gln Lys Ser Gln Val Val Gly Asp Val Asp Asp Ile 
705                 710                 715                 720 
Glu Ala Val Val His Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly Asp Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Arg Gly Arg Ser Gln Ser Gln Gln Arg Leu Lys Lys Leu 
    770                 775                 780                 
Gln Asn Ser Leu Lys Glu Leu Gly Ser Asn Leu Asn Glu Glu Lys Pro 
785                 790                 795                 800 
Val Glu Asn Ser His Leu Gln Asn Asp Gln Leu Phe Leu Tyr Tyr Ile 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Asp Glu Leu Asp Ile Asp His 
            820                 825                 830         
Leu Ser Asp Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Leu Asp Ile Val Arg Ala Arg Lys 
865                 870                 875                 880 
Ala Glu Trp Val Arg Leu Tyr Lys Ser Gly Leu Ile Ser Lys Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Ala Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Arg Asp 
    930                 935                 940                 
Glu Asn Asp Lys Val Ile Arg Asp Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Asp Phe Glu Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Ala Ser Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Lys Lys Tyr Asp Val Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser His Ser Glu Met Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Thr Val Val Lys Tyr Ala Asp Gly Lys 
                1045                1050                1055    
Val Ile Glu Arg Pro Val Ile Glu Thr Asn Lys Asp Thr Gly Glu Ile 
            1060                1065                1070        
Ala Trp Asn Lys Gln Thr Asp Phe Glu Lys Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Thr Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Val Tyr Trp Asp Thr Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Phe Val Val Ala Asp Val Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Ser Ile 
        1155                1160                1165            
Met Glu Arg Ser Phe Phe Glu Glu Asn Pro Val Glu Phe Leu Glu Asn 
    1170                1175                1180                
Lys Gly Tyr His Asn Ile Arg Glu Asp Lys Leu Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Phe Glu Gly Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Ser Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Gly His Leu 
            1220                1225                1230        
Val Glu Leu Leu Tyr His Ala His Arg Ile Asp Asn Phe Asn Gly Ser 
        1235                1240                1245            
Thr Glu Tyr Leu Glu Gln Lys Gln Asn Tyr Val Ser Glu His Lys Lys 
    1250                1255                1260                
Glu Phe Glu Lys Val Leu Ser Cys Val Glu Asp Phe Ala Asn Leu Tyr 
1265                1270                1275                1280
Val Asp Val Glu Lys Asn Leu Ser Lys Ile Arg Ala Val Ala Asp Ser 
                1285                1290                1295    
Met Asp Asn Phe Ser Ile Glu Glu Ile Ser Asn Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Leu Thr Ala Leu Gly Ala Pro Ala Asp Phe Asn Phe Leu Gly 
        1315                1320                1325            
Glu Lys Ile Pro Arg Lys Arg Tyr Thr Ser Thr Lys Glu Cys Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Glu 
                1365            

<210> 226
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #107

<400> 226
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Leu Leu Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Gln Asn Leu Pro Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Lys Thr Arg Glu Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp His Leu Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Leu Asp Tyr Leu Glu Lys Glu Phe Asp Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu 
705                 710                 715                 720 
Lys Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Asp Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Ala Arg Phe Asn Thr Glu Leu Asp 
    930                 935                 940                 
Glu Asn Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Pro Lys Tyr Asn Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Lys Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Met Ile Glu Val Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Arg 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Ile Gln Glu Asn Lys Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile His Lys Val Asn Gly Glu 
        1235                1240                1245            
Pro Glu His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Gly 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Val Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 227
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #108

<400> 227
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Gln Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Asp Phe Val Glu Val Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Leu Leu Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asp Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Gln Asn Leu Pro Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Ala Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Arg Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Lys Thr Arg Glu Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Gly Leu Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Leu Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Tyr Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asp Ser Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Leu Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ser 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu 
705                 710                 715                 720 
Lys Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Asp Ala Ile Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Asp Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Leu Asp 
    930                 935                 940                 
Glu Asn Asp Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Lys Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Pro Lys Tyr Asn Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Lys Lys Ala Thr Ala Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Met Ile Glu Val Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys His Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ala Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Arg 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Ile Gln Glu Asn Lys Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile His Lys Val Asn Gly Glu 
        1235                1240                1245            
Pro Glu His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asp Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Val Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 228
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #109

<400> 228
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Val Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Ser His Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Gly Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Thr Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Thr Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Glu Leu Asn Ala 
                165                 170                 175     
Glu Asn Thr Asp Val Gln Lys Leu Phe Gln Asp Phe Val Gly Val Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Val Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Leu Leu Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Glu Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Glu Leu Glu Val Leu Leu Ala Gln Ile Gly Asp Asn Tyr Ala Glu 
        275                 280                 285             
Leu Phe Leu Ser Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Val Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asn Glu His Gln Met Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Arg Gln Lys Leu Ser Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Val Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
        355                 360                 365             
Gln Glu Ala Phe Tyr Lys Tyr Leu Lys Gly Leu Leu Asn Lys Ile Glu 
    370                 375                 380                 
Gly Ser Gly Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Arg Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro Phe 
            420                 425                 430         
Leu Ala Asp Asn Gln Asp Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Ser Arg Lys Ser Ala Asp Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Ile Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asn Gln Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Lys Thr Glu Gln Gly Lys Thr Ala Glu Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr 
545                 550                 555                 560 
Lys Asp Lys Leu Met Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Leu Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Tyr Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Arg Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Ser Lys Asn Glu Lys Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Asn Tyr Ser 
625                 630                 635                 640 
Asp Leu Leu Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Glu Leu Ile His Gly Ile Arg Asn 
            660                 665                 670         
Lys Glu Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Asn 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu 
705                 710                 715                 720 
Asn Gln Val Val Ser Asp Ile Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Ile Met Gly 
            740                 745                 750         
Gly His Gln Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Phe Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Gly Leu 
    770                 775                 780                 
Thr Asp Ser Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Ser Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Tyr 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Lys Asp Val Val Arg Lys Met Lys 
865                 870                 875                 880 
Ser Tyr Trp Ser Lys Leu Leu Ser Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Thr Asp 
    930                 935                 940                 
Glu Asn Asn Lys Lys Ile Arg Gln Val Lys Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Ile Gly Lys Ala Leu Leu Gly Val Tyr Pro Gln Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Pro His Phe His Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Gly His Lys Glu Asn Lys Ala Thr Ala Lys Lys Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Lys Asp Asp Val Arg Thr Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Met Ile Glu Val Asn Lys Lys Asn Gly Glu Ile 
            1060                1065                1070        
Ile Trp Lys Lys Asp Glu His Ile Ser Asn Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Phe Tyr Trp Asp Thr Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Ile Val Ala Tyr Ser Ile Leu Val Ile Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ser Lys Lys Leu Lys Thr Val Lys Asp Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Thr Thr Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Arg 
    1170                1175                1180                
Lys Gly Tyr Arg Asn Val Gln Glu Glu Asn Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Arg Glu Leu Gln Lys Gly Asn Glu Ile Val Leu Pro Asn His Leu 
            1220                1225                1230        
Gly Thr Leu Leu Tyr His Ala Lys Asn Ile His Lys Val Asp Gly Glu 
        1235                1240                1245            
Pro Lys His Leu Glu Gln Lys Gln Asp Tyr Val Glu Lys His Lys Asp 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asp Val Val Ser Asn Phe Ser Lys Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Glu Ser Asn Leu Glu Lys Ile Lys Glu Leu Tyr Ala Gln 
                1285                1290                1295    
Asn Asn Asn Glu Asp Ile Lys Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Ile Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Ile Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Gly Asp 
                1365            

<210> 229
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #110

<400> 229
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Leu Ala Glu Glu Val Ala Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Asp Asp Leu Asp Val 
                165                 170                 175     
Arg Asn Thr Asp Val Gln Lys Leu Phe Gln Asp Phe Leu Glu Val Tyr 
            180                 185                 190         
Asp Ser Thr Phe Glu Asp Ser His Leu Ser Glu Gln Asn Ile Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Lys Leu Glu Arg 
    210                 215                 220                 
Leu Leu Ala His Phe Pro Asn Gln Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asp Glu His Gln Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Ala Asn Leu Pro Asp Lys Tyr Lys Glu Val Phe Ser 
            340                 345                 350         
Asp Ala Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His Leu 
                405                 410                 415     
Ala Glu Met Lys Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Asn Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gln Gly Glu Thr Arg Glu Phe Phe Asp Ala Asn Met 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Gly Leu Phe Lys Glu Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Leu Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Ile Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Tyr Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asp Ser Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Lys Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Leu Phe Thr Lys Glu Gln Leu Lys Lys Leu Ser Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Arg 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Asp Ser Asp Asn Leu 
705                 710                 715                 720 
Lys Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly Tyr Asn Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Ile Lys Asn Leu Gly Ser Asp Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Asp Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Ala Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Leu Asp 
    930                 935                 940                 
Glu Asn Asp Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Lys Ala Leu Leu Thr Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Pro Lys Tyr Asn Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Lys Ser Ala Thr Glu Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Lys Val Lys Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Met Ile Glu Val Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys His Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Leu Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Leu Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Arg Phe Glu Lys Asn Pro Val Ala Phe Leu Glu Arg 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Ile Gln Glu Asn Lys Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Lys Leu Leu Tyr His Ala Lys Arg Ile Asn Lys Val Asn Gly Glu 
        1235                1240                1245            
Pro Glu His Ile Glu Gln Lys Gln Glu Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Lys Asp Leu Leu His Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Asn Glu Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Glu Asp Ala Ser Val Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Val Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 230
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #111

<400> 230
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         
Gly Asp Arg His Pro Ile Phe Gly Asn Ile Ala Asp Glu Val Lys Tyr 
        115                 120                 125             
His Glu Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Asp Asp Pro Leu Asp Val 
                165                 170                 175     
Arg Asn Thr Asp Ser Gln Lys Leu Phe Gln Glu Phe Leu Glu Ala Tyr 
            180                 185                 190         
Asp Ser Thr Gln Glu Asp Ser His Leu Ser Glu Gln Lys Ile Asp Ile 
        195                 200                 205             
Ser Asp Ile Ile Thr Glu Lys Ile Ser Lys Ser Arg Lys Val Glu Ala 
    210                 215                 220                 
Leu Leu Ala His Phe Pro Asn Gln Lys Lys Asn Ser Phe Phe Gly Asn 
225                 230                 235                 240 
Leu Val Ser Leu Val Phe Gly Leu Met Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Val Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Asn Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Gln Arg Tyr Asp Glu His Asn Lys Asp Leu Ala Gln Leu Lys 
                325                 330                 335     
Asp Phe Ile Lys Ala Asn Leu Pro Asp Val Phe Lys Glu Val Phe Ser 
            340                 345                 350         
Asp Ala Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Gly Lys Val Asp 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Ile Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His Leu 
                405                 410                 415     
Ala Glu Met Lys Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Gln Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Ala Ser Phe Asn Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Ile Asp Lys Ser Ser Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Asn Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Thr Gly Glu Ala Arg Glu Phe Phe Asp Ala Asn Leu 
    530                 535                 540                 
Lys Gln Glu Ile Phe Asp Gly Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Leu Asp Phe Leu Glu Lys Asn Phe Asp Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Ile Thr Gly Leu Asp Lys Glu Phe Asn Ala Ser Tyr Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Glu Phe Met Asp 
        595                 600                 605             
Asp Ser Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Lys Tyr Ala 
625                 630                 635                 640 
Asp Leu Phe Asp Lys Lys Val Ile Asp Lys Leu Ser Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Arg 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Asp Glu Ile Ala Lys Ala Gln Val Ile Gly Asp Ser Asp Asp Leu 
705                 710                 715                 720 
His Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly Tyr Asn Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ala Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ala Ile Lys Asn Leu Gly Ser Asp Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Val Ser Ser Ala Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Asn Asp Val Val Lys Arg Met Lys 
865                 870                 875                 880 
Ser Phe Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Ala Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Arg Glu Phe Asp 
    930                 935                 940                 
Glu Asn Asp Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Lys Ala Leu Leu Thr Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Pro Lys Tyr Asn Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Lys Ser Ala Thr Glu Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Met Phe Lys Thr Lys Val Lys Leu Ala Asp Gly Arg 
                1045                1050                1055    
Val Ile Glu Arg Pro Met Ile Glu Val Asn Glu Glu Thr Gly Glu Ile 
            1060                1065                1070        
Ala Trp Asp Lys Thr Lys His Phe Ala Thr Val Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ala Arg Lys Asn Tyr Leu Asp Thr Gln Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Leu Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Arg Phe Glu Asn Asn Ser Val Ala Phe Leu Glu Arg 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Ile Gln Glu Asn Lys Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Lys Leu Leu Tyr His Ala Lys His Ile Asn Ser Val Asn Gly Glu 
        1235                1240                1245            
Pro Glu His Ile Glu Gln Lys Gln Asn Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Lys Asp Leu Leu His Tyr Ile Arg Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Asn Glu Leu Phe Glu Glu 
                1285                1290                1295    
Asn Glu Glu Asp Ser Val Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Val Leu Ser 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 231
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #112

<400> 231
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Glu Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Met 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Glu Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Ala Glu Asp Lys Arg 
            100                 105                 110         
Gly Glu Arg His Pro Ile Phe Gly Asn Ile Ala Asp Glu Val Lys Tyr 
        115                 120                 125             
His Glu Glu Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly His Phe Leu Ile Asp Glu Pro Ile Asp Ile 
                165                 170                 175     
Arg Asn Thr Asp Ser Gln Lys Leu Phe Gln Glu Phe Leu Glu Ala Tyr 
            180                 185                 190         
Asp Ser Ile Gln Glu Asp Ser His Leu Ser Glu Gln Lys Thr Asp Ile 
        195                 200                 205             
Ser Asp Ile Ile Thr Ala Lys Ile Ser Lys Ser Arg Lys Val Glu Ala 
    210                 215                 220                 
Leu Leu Ala Gln Phe Pro Asn Gln Lys Lys Asn Ser Phe Phe Gly Asn 
225                 230                 235                 240 
Leu Val Ser Leu Val Phe Gly Leu Met Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Met Ile Gly Asp Glu Tyr Ala Asp 
        275                 280                 285             
Val Phe Val Ala Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Thr Asn Asp Leu Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Asp Glu His Asn Lys Asp Lys Gln Leu Leu Lys 
                325                 330                 335     
Asp Phe Ile Arg Ala Asn Ile Gly Asp Val Phe Lys Glu Val Phe Tyr 
            340                 345                 350         
Asp Ala Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Gly Lys Val Asp 
    370                 375                 380                 
Gly Ser Glu Tyr Phe Ile Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Val His Leu 
                405                 410                 415     
Ala Glu Met Lys Ala Ile Leu Arg Arg Gln Gly Glu Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Ala Glu Lys Ile Gln Gln Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Ala Ser Phe Asn Ser Asn Glu Lys Ile Thr Pro Trp Asn Phe Asp Asp 
465                 470                 475                 480 
Val Ile Asp Lys Ser Ser Ser Ala Gln Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Asn Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Thr Gly Glu Ala Arg Glu Phe Phe Asp Ala Asn Leu 
    530                 535                 540                 
Lys Lys Glu Ile Phe Asp Gly Leu Phe Lys Lys Asn Arg Lys Val Thr 
545                 550                 555                 560 
Lys Lys Lys Leu Leu Asp Phe Leu Glu Lys Asn Phe Asp Glu Phe Arg 
                565                 570                 575     
Ile Val Asp Ile Thr Gly Leu Asp Asn Glu Phe Asn Ala Ser Tyr Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Glu Phe Met Asp 
        595                 600                 605             
Asp Ser Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Leu Lys Tyr Glu 
625                 630                 635                 640 
Asp Leu Phe Asp Lys Lys Val Ile Asp Lys Leu Ser Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Lys Asp 
            660                 665                 670         
Lys Gln Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly His 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Asn Leu Ser Phe 
    690                 695                 700                 
Lys Asp Glu Ile Ala Lys Ala Gln Val Ile Gly Asp Gly Asp Asp Leu 
705                 710                 715                 720 
His Gln Val Val Gln Glu Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly Tyr Asn Pro Glu Gln Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ala Arg Gly Arg Arg Asn Ser Gln Gln Arg Leu Gly Arg Leu 
    770                 775                 780                 
Lys Glu Ala Ile Gln Asp Leu Gly Ser Asp Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Asn Gln Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Thr Leu Asp Ile Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Leu Asp Asn Arg Val Leu Val Ser Ser Ala Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Asn Asp Val Val Lys Arg Met Lys 
865                 870                 875                 880 
Ser Phe Trp Ser Lys Leu Leu Asn Ala Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Ala Asp Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Arg Glu Phe Asp 
    930                 935                 940                 
Glu Asn Asp Lys Arg Ile Arg Lys Val Lys Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Lys Ala Leu Leu Thr Arg Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Glu Tyr Pro Lys Tyr Asn Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Lys Ser Ala Thr Glu Lys Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Met Phe Lys Thr Thr Ile Lys Leu Ala Asp Gly Arg 
                1045                1050                1055    
Val Ile Glu Arg Pro Met Ile Glu Ala Asn Glu Glu Thr Gly Glu Ile 
            1060                1065                1070        
Ala Trp Asp Lys Thr Lys His Phe Ala Thr Val Lys Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ala Arg Lys Asn Tyr Leu Asn Thr Gln Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Phe Val Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ser Lys Lys Leu Lys Thr Val Lys Glu Met Ile Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Arg Phe Glu Ser Asn Ser Val Ala Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Gln Asn Ile Arg Glu Asn Thr Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Lys Leu Leu Tyr His Ala Lys His Ile Asn Ser Val Asn Gly Lys 
        1235                1240                1245            
Pro Glu His Ile Glu Gln Lys Gln Asn Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Thr Asp Leu Leu His His Ile Arg Asp Phe Ser Glu Lys Asn 
1265                1270                1275                1280
Ile Leu Ala Ala Lys Asn Leu Glu Lys Ile Asn Glu Ala Phe Glu Glu 
                1285                1290                1295    
Asn Glu Glu Asp Ser Val Asn Glu Leu Ala Gln Ser Phe Ile Ser Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Phe Asp 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Val Leu Ser 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Glu 
                1365            

<210> 232
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #113

<400> 232
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Arg Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Asn Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys His Pro Ile Phe Gly Asn Leu Ala Glu Glu Lys Ala Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Ser 
                165                 170                 175     
Glu Asn Asn Asp Val Gln Lys Leu Phe Gln Asp Phe Val Glu Ile Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Gly Ser His Leu Ser Glu Gln Asn Ala Asp Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Glu Lys Ile Ser Lys Ser Arg Arg Leu Glu Arg 
    210                 215                 220                 
Ile Leu Lys His Phe Pro Asn Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Phe Leu Ala Leu Ile Leu Gly Leu Gln Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Asp Leu Ser Glu Asp Ala Lys Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Gln Asn Leu Pro Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Ser Lys Ile Glu 
    370                 375                 380                 
Gly Ser Asp Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Asp Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Met Arg Asp Tyr Gln Phe Leu Asp Ser Gly Gln 
    530                 535                 540                 
Lys Lys Glu Ile Val Asn His Leu Phe Lys Glu Lys Arg Lys Val Thr 
545                 550                 555                 560 
Glu Lys Asp Ile Ile Asp Tyr Leu Glu Asn Glu Ile Asp Glu Tyr Asp 
                565                 570                 575     
Gly Val Glu Leu Lys Gly Leu Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Leu Lys Asp Lys Glu Phe Leu Asp 
        595                 600                 605             
Asp Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Lys Tyr Ser 
625                 630                 635                 640 
Asp Ile Phe Thr Lys Glu Gln Leu Lys Lys Leu Glu Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Thr 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu 
705                 710                 715                 720 
Lys Gln Val Val Gln Asp Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Tyr Lys Arg Leu 
    770                 775                 780                 
Glu Asp Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Thr Asp Asn Gln Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Gln 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Glu Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Arg Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Glu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Ala Arg Phe Asn Thr Glu Leu Asp 
    930                 935                 940                 
Glu Asn Asp Lys Arg Ile Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Pro Lys Tyr Asn Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Lys Lys Ala Thr Glu Lys Val Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Lys Glu Val Lys Tyr Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Met Ile Glu Val Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Leu Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Ile Ala Tyr Ser Val Leu Val Ile Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ala Ala Phe Glu Lys Asn Pro Ile Ala Phe Leu Glu Arg 
    1170                1175                1180                
Lys Gly Tyr Lys Asn Ile Gln Glu Asn Asn Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Met Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile His Lys Ile Asn Gly Glu 
        1235                1240                1245            
Pro Ile His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asn Tyr Ile Ser Asp Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Glu Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Asp Asp Ala Asp Ile Asn Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Leu Gly 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Thr Glu Ile Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 233
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #114

<400> 233
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Arg Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Thr Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys Tyr Pro Ile Phe Gly Asn Leu Ala Glu Glu Lys Ala Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Asp Phe Asn Ser 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Ile Phe Gln Asp Phe Val Glu Ile Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Gly Ser His Leu Ser Glu Gln Asn Ala Gln Val 
        195                 200                 205             
Glu Glu Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 
Ile Leu Lys Leu Phe Pro Asn Glu Lys Asn Asn Gly Leu Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     
Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Glu Glu His Gln Lys Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         
Asp Lys Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Lys Tyr Leu Lys Asn Leu Leu Ser Lys Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Asp Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met His Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Lys Ser Asp Glu Lys Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Met Arg Asp Tyr Gln Phe Leu Asp Ser Gly Gln 
    530                 535                 540                 
Lys Lys Glu Ile Val Asn His Leu Phe Lys Glu Lys Arg Lys Val Thr 
545                 550                 555                 560 
Glu Lys Asp Ile Ile Asp Tyr Leu His Asn Glu Ile Asp Gly Tyr Asp 
                565                 570                 575     
Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Leu Asp 
        595                 600                 605             
Asp Ala Glu Asn Glu Ala Ile Leu Glu Asp Ile Val His Thr Leu Thr 
    610                 615                 620                 
Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Lys Tyr Asp 
625                 630                 635                 640 
Asn Ile Phe Asp Lys Glu Val Leu Lys Lys Leu Thr Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Thr 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Glu Glu Ile Gln Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Val 
705                 710                 715                 720 
Lys Gln Val Val Gln Asp Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Asn Gln Gly Lys Arg Asn Ser Gln Gln Arg Tyr Lys Arg Leu 
    770                 775                 780                 
Glu Asp Ala Leu Lys Asn Leu Gly Ser Asn Ile Leu Lys Glu Asn Pro 
785                 790                 795                 800 
Thr Asp Asn Asn Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Gln 
            820                 825                 830         
Leu Ser Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Ala Asp Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Arg Lys Arg Lys 
865                 870                 875                 880 
Ser Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu Glu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Ile Leu Asp Ala Arg Phe Asn Thr Glu Leu Asp 
    930                 935                 940                 
Glu Asn Asp Lys Arg Ile Arg Thr Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Gln Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Pro Lys Tyr Asn Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Tyr Arg Glu Arg Lys Lys Ala Thr Glu Lys Val Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Lys Glu Val Lys Tyr Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Met Ile Glu Val Asn Lys Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asn Lys Glu Lys Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Tyr Leu Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Ile Ala Tyr Ser Val Leu Val Ile Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ala Ala Phe Glu Lys Asn Pro Ile Thr Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Lys Asn Ile Gln Glu Asn Asn Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Ile Val Leu Pro Ala His Leu 
            1220                1225                1230        
Val Thr Leu Leu Tyr His Ala Lys Arg Ile His Lys Ile Asn Gly Glu 
        1235                1240                1245            
Pro Ile His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asn Tyr Ile Ser Glu Phe Ser Glu Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Lys Asn Leu Glu Lys Ile Lys Ser Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Glu Asp Ala Asp Ile Asp Glu Leu Ala Ser Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Leu Gly 
        1315                1320                1325            
Lys Asn Ile Asp Arg Lys Arg Tyr Thr Ser Thr Ser Glu Ile Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 234
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #115

<400> 234
Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Ile Thr Ala Glu Gly Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala Thr Leu Asp Asp Ala 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Pro Asp Asp Lys Arg 
            100                 105                 110         
Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val Glu Glu Lys Ala Tyr 
        115                 120                 125             
His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 
Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Phe Asn Ser 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp Phe Leu Asp Thr Tyr 
            180                 185                 190         
Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu Asn Ser Lys Gln Leu 
        195                 200                 205             
Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp Arg 
    210                 215                 220                 
Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser Gly Ile Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys Tyr Phe 
                245                 250                 255     
Asn Leu Asp Glu Lys Ala Pro Leu His Phe Ser Lys Glu Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly Asp Asp Tyr Ser Asp 
        275                 280                 285             
Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Asn Gly Thr Glu Ala Pro Leu Ser Ser Ala 
305                 310                 315                 320 
Met Ile Met Arg Tyr Lys Glu His Glu Glu Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Ala Tyr Ile Arg Asn Ile Ser Leu Glu Thr Tyr Asn Glu Val Phe Lys 
            340                 345                 350         
Asp Asn Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Asp Phe Tyr Val Tyr Leu Lys Asn Leu Leu Ser Asn Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala Lys Phe Tyr Pro Phe 
            420                 425                 430         
Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro Trp Asn Phe Glu Asp 
465                 470                 475                 480 
Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Phe Ile Ala Glu Ser Met Arg Asp Tyr Gln Phe Leu Asp Ser Lys Gln 
    530                 535                 540                 
Lys Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp Lys Arg Lys Val Thr 
545                 550                 555                 560 
Asp Lys Asp Ile Ile Glu Tyr Leu His Ala Glu Ile Asp Gly Tyr Asp 
                565                 570                 575     
Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ser Ser Leu Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys Glu Phe Leu Asp 
        595                 600                 605             
Asp Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile His Thr Leu Thr 
    610                 615                 620                 
Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ser Lys Phe Glu 
625                 630                 635                 640 
Asn Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Glu Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ile 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ala Leu Ser Phe 
    690                 695                 700                 
Lys Lys Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp Lys Asp Asn Ile 
705                 710                 715                 720 
Lys Gln Val Val Gln Ala Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly Arg Lys Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Tyr Thr Asn Gln Gly Lys Ser Asn Ser Gln Gln Arg Leu Lys Arg Leu 
    770                 775                 780                 
Glu Glu Ser Leu Lys Glu Leu Gly Ser Lys Ile Leu Lys Glu Asn Ile 
785                 790                 795                 800 
Ile Asp Asn Asn Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Arg 
            820                 825                 830         
Leu Ser Asn Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Leu Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Lys Val Leu Val Ser Ser Ala Ser Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asp Val Pro Ser Leu Glu Val Val Lys Lys Arg Lys 
865                 870                 875                 880 
Thr Leu Trp Tyr Gln Leu Leu Lys Ser Lys Leu Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Arg Glu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Arg Leu Leu Asp Glu Arg Phe Asn Asn Lys Lys Asp 
    930                 935                 940                 
Glu Asn Asn Lys Thr Val Arg Thr Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Ser Leu Val Ser Gln Phe Arg Lys Asp Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Phe His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Ser Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Pro Lys Tyr Asn Ser Arg Lys Leu Ile Ala Lys 
    1010                1015                1020                
Ser Phe Arg Glu Arg Lys Ser Ala Thr Glu Lys Val Tyr Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Ile Phe Lys Lys Ser Ile Ser Leu Ala Asp Gly Thr 
                1045                1050                1055    
Val Ile Glu Arg Pro Leu Ile Glu Val Asn Glu Glu Thr Gly Glu Ser 
            1060                1065                1070        
Val Trp Asn Lys Glu Ser Asp Leu Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Lys Lys Val Glu Glu Gln Asn His Gly 
    1090                1095                1100                
Leu Asp Arg Lys Gly Leu Phe Asn Ala Asn Leu Ser Ser Lys Pro Lys 
1105                1110                1115                1120
Pro Asn Ser Glu Tyr Leu Asp Pro Lys Lys Tyr Gly Gly Tyr Ala Gly 
                1125                1130                1135    
Ile Ser Asn Ser Phe Ala Val Leu Val Lys Gly Thr Ile Glu Lys Gly 
            1140                1145                1150        
Ala Lys Lys Lys Ile Thr Asn Val Leu Glu Phe Gln Gly Ile Ser Ile 
        1155                1160                1165            
Leu Asp Arg Ile Thr Tyr Arg Lys Asp Lys Leu Asn Phe Leu Leu Glu 
    1170                1175                1180                
Lys Gly Tyr Lys Asp Ile Glu Glu Asn Leu Ile Ile Glu Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Ser Asp Gly Ser Arg Arg Met Leu Ala Ser 
                1205                1210                1215    
Ile Gly Glu Ile His Lys Gly Asn Gln Ile Phe Leu Ser Gln Lys Phe 
            1220                1225                1230        
Val Lys Leu Leu Tyr His Ala Lys Arg Ile Ser Asn Thr Ile Gly Asn 
        1235                1240                1245            
Glu Asn His Arg Glu Gln Lys Gln Lys Tyr Val Glu Asn His Lys Lys 
    1250                1255                1260                
Glu Phe Glu Glu Leu Phe Tyr Tyr Ile Leu Glu Phe Asn Glu Asn Tyr 
1265                1270                1275                1280
Val Gly Ala Lys Lys Asn Gly Glu Leu Leu Lys Ser Ala Phe Gln Ser 
                1285                1290                1295    
Trp Gln Asn His Ser Ile Asp Glu Leu Cys Ser Ser Phe Ile Gly Leu 
            1300                1305                1310        
Phe Glu Leu Thr Ser Arg Gly Ser Ala Ala Asp Phe Glu Phe Leu Gly 
        1315                1320                1325            
Val Lys Ile Pro Arg Tyr Arg Tyr Thr Pro Ser Ser Leu Leu Lys Asp 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Val Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ala Lys Leu Gly Glu Asp 
                1365            

<210> 235
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #116

<400> 235
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys Arg Phe Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Ser Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu Tyr 
        115                 120                 125             
His Lys Gln Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ala Phe Asp Ile 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln Val 
        195                 200                 205             
Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 
Val Leu Lys Leu Phe Pro Asp Glu Lys Ser Thr Gly Leu Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     
Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Asp Glu Val Phe Ser 
            340                 345                 350         
Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu Tyr Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Asn Ser Asp Glu Ala Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Ile Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly Gln 
    530                 535                 540                 
Lys Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val Thr 
545                 550                 555                 560 
Glu Lys Asp Ile Ile His Tyr Leu His Asn Glu Val Asp Gly Tyr Asp 
                565                 570                 575     
Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp 
        595                 600                 605             
Asp Ala Lys Asn Glu Ala Ile Leu Glu Asn Ile Val His Thr Leu Thr 
    610                 615                 620                 
Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp 
625                 630                 635                 640 
Ser Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Thr Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Lys 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe 
    690                 695                 700                 
Lys Glu Ile Ile Gln Lys Ala Gln Val Ile Gly Lys Thr Asp Asp Val 
705                 710                 715                 720 
Lys Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile 
    770                 775                 780                 
Glu Asp Ala Leu Lys Asn Leu Ala Ser Gly Ile Leu Lys Glu Asn Pro 
785                 790                 795                 800 
Thr Asp Asn Asn Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Glu Ala Leu Asp Ile Asn Gln 
            820                 825                 830         
Leu Ser Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys Asp Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Gln Lys Arg Lys 
865                 870                 875                 880 
Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Glu Arg Lys 
                885                 890                 895     
Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp Glu Arg Asp 
            900                 905                 910         
Lys Val Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Val Asn 
    930                 935                 940                 
Glu Lys Asp Lys Lys Asn Arg Thr Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Lys Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Lys Ala Ile Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Gln Lys Tyr Asp Leu Lys Arg Tyr Ile Ser Arg 
    1010                1015                1020                
Ser Pro Lys Glu Val Glu Lys Ala Thr Glu Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Leu Asn Phe Phe Lys Glu Glu Val His Tyr Ala Asp Gly Thr 
                1045                1050                1055    
Ile Val Lys Arg Glu Asn Ile Glu Tyr Ser Lys Asp Thr Gly Glu Ile 
            1060                1065                1070        
Ala Trp Asn Lys Glu Lys Asp Phe Ala Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Leu Pro Gln Val Asn Ile Val Lys Lys Thr Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Pro Arg Lys Ile Ser Leu Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Ile Ala Tyr Ser Val Leu Val Ile Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Thr Val Lys Glu Leu Val Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Lys Ala Ala Phe Glu Glu Asn Pro Ile Thr Phe Leu Glu Glu 
    1170                1175                1180                
Lys Gly Tyr Lys Asn Ile Arg Lys Asn Asn Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Ile Val Leu Pro Val His Leu 
            1220                1225                1230        
Thr Thr Leu Leu Tyr His Ala Lys Arg Ile His Lys Ile Asn Gly Glu 
        1235                1240                1245            
Pro Ile His Leu Glu Gln Lys Gln Glu Tyr Val Glu Lys His Arg Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Asn Leu Ile Ser Glu Phe Ser Gln Lys Tyr 
1265                1270                1275                1280
Ile Leu Ala Asp Asn Asn Leu Glu Lys Ile Lys Ser Leu Tyr Glu Glu 
                1285                1290                1295    
Asn Glu Asp Ala Asp Ile Asp Gln Leu Ala Asn Ser Phe Ile Asn Leu 
            1300                1305                1310        
Leu Thr Phe Thr Ala Leu Gly Ala Pro Ala Ala Phe Lys Phe Leu Gly 
        1315                1320                1325            
Lys Asp Ile Asp Arg Lys Arg Tyr Thr Ser Val Ser Glu Ile Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 236
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> 1368 residue - joint ancestral sequence reconstruction method–
      node #117

<400> 236
Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Lys His Phe Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Glu Gly Thr Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Leu Arg 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Ser Lys Val Asp Ser Ser 
                85                  90                  95      
Phe Phe His Arg Leu Asp Asp Ser Phe Leu Val Pro Glu Asp Lys Arg 
            100                 105                 110         
Gly Ser Lys Tyr Pro Ile Phe Ala Thr Leu Ala Glu Glu Lys Glu Tyr 
        115                 120                 125             
His Lys Lys Phe Pro Thr Ile Tyr His Leu Arg Lys His Leu Ala Asp 
    130                 135                 140                 
Ser Lys Glu Lys Thr Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Tyr Glu Glu Ser Phe Asp Ile 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Ile Phe Asn Glu Phe Ile Ser Ile Tyr 
            180                 185                 190         
Asp Asn Thr Phe Glu Gly Ser Ser Leu Ser Gly Gln Asn Ala Gln Val 
        195                 200                 205             
Glu Ala Ile Phe Thr Asp Lys Ile Ser Lys Ser Ala Lys Arg Glu Arg 
    210                 215                 220                 
Val Leu Lys Leu Phe Ser Asp Glu Lys Ser Thr Gly Leu Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     
Asp Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Asn Leu Leu Gly Gln Ile Gly Asp Asp Phe Ala Asp 
        275                 280                 285             
Leu Phe Leu Val Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Ile Leu Thr Val Thr Asp Pro Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Glu Arg Tyr Glu Asn His Gln Lys Asp Leu Ala Ala Leu Lys 
                325                 330                 335     
Gln Phe Ile Lys Asn Asn Leu Pro Glu Lys Tyr Asp Glu Val Phe Ser 
            340                 345                 350         
Asp Gln Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Thr 
        355                 360                 365             
Gln Glu Ala Phe Tyr Lys Tyr Ile Lys Asn Leu Leu Ser Lys Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Asn Ala Ile Leu Arg Arg Gln Gly Glu His Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Lys Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Arg Asp Phe Ala Trp 
    450                 455                 460                 
Leu Thr Arg Asn Ser Asp Gln Ala Ile Arg Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Ile Val Asp Lys Ala Ser Ser Ala Glu Asp Phe Ile Asn Lys Met Thr 
                485                 490                 495     
Asn Tyr Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Ala Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Phe Ile Ala Glu Gly Leu Arg Asp Tyr Gln Phe Leu Asp Ser Gly Gln 
    530                 535                 540                 
Lys Lys Gln Ile Val Asn Gln Leu Phe Lys Glu Lys Arg Lys Val Thr 
545                 550                 555                 560 
Glu Lys Asp Ile Ile His Tyr Leu His Asn Glu Val Asp Gly Tyr Asp 
                565                 570                 575     
Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ala Ser Leu Ser 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Glu Phe Met Asp 
        595                 600                 605             
Asp Ala Lys Asn Glu Ala Ile Leu Glu Asn Ile Ile His Thr Leu Thr 
    610                 615                 620                 
Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ala Gln Tyr Asp 
625                 630                 635                 640 
Ser Leu Phe Asp Glu Lys Val Ile Lys Ala Leu Thr Arg Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Thr Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Asp 
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Gly Leu Ser Phe 
    690                 695                 700                 
Lys Glu Ile Ile Gln Lys Ala Gln Val Val Gly Lys Thr Asp Asp Val 
705                 710                 715                 720 
Lys Gln Val Val Gln Glu Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Gly His Asn Pro Glu Ser Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Ala Arg Gly Lys Lys Asn Ser Gln Gln Arg Tyr Lys Arg Ile 
    770                 775                 780                 
Glu Asp Ala Leu Lys Asn Leu Ala Pro Gly Ile Leu Lys Glu Asn Pro 
785                 790                 795                 800 
Thr Asp Asn Ile Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Lys Asp Met Tyr Thr Gly Lys Ala Leu Asp Ile Asn Gln 
            820                 825                 830         
Leu Ser Ser Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             
Asp Asp Ser Leu Asp Asn Arg Val Leu Thr Ser Ser Lys Asp Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Ile Glu Val Val Gln Lys Arg Lys 
865                 870                 875                 880 
Ala Phe Trp Gln Gln Leu Leu Asp Ser Lys Leu Ile Ser Glu Arg Lys 
                885                 890                 895     
Phe Asn Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Asp Glu Arg Asp 
            900                 905                 910         
Lys Val Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ala Arg Phe Asn Thr Glu Val Asn 
    930                 935                 940                 
Glu Lys Asp Lys Lys Asn Arg Thr Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Lys Leu Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Ala Lys Ala Ile Leu Lys Lys Tyr Pro Lys Leu Glu Pro Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Gln Lys Tyr Asp Leu Lys Arg Tyr Ile Ser Arg 
    1010                1015                1020                
Ser Pro Lys Asp Val Glu Lys Ala Thr Glu Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Leu Asn Phe Phe Lys Glu Glu Val His Tyr Ala Asp Gly Thr 
                1045                1050                1055    
Ile Val Lys Arg Glu Asn Ile Glu Tyr Ser Asn Asp Thr Gly Glu Ile 
            1060                1065                1070        
Ala Trp Asn Lys Glu Lys Asp Phe Ala Thr Ile Lys Lys Val Leu Ser 
        1075                1080                1085            
Leu Pro Gln Val Asn Ile Val Lys Lys Thr Glu Glu Gln Thr Gly Gly 
    1090                1095                1100                
Leu Phe Asp Asn Asn Ile Val Ser Lys Lys Asp Ala Ser Lys Leu Ile 
1105                1110                1115                1120
Pro Ile Lys Ser Ser Leu Ser Pro Glu Lys Tyr Gly Gly Tyr Ala Arg 
                1125                1130                1135    
Pro Thr Ile Ala Tyr Ser Val Leu Val Ile Ala Asp Ile Glu Lys Gly 
            1140                1145                1150        
Lys Ala Lys Lys Leu Lys Arg Ile Lys Glu Met Val Gly Ile Thr Ile 
        1155                1160                1165            
Gln Asp Lys Lys Lys Phe Glu Ala Asn Pro Ile Thr Tyr Leu Glu Glu 
    1170                1175                1180                
Arg Gly Tyr Lys Asn Ile Asn Ser Asn Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Phe Asn Asp Gly Gln Arg Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ser Ile Glu Leu Gln Lys Gly Asn Glu Leu Ile Leu Pro Tyr His Leu 
            1220                1225                1230        
Thr Ala Leu Leu Tyr His Ala Gln Arg Ile Asn Lys Ile Asn Gly Glu 
        1235                1240                1245            
Pro Ile His Lys Glu Gln Lys Gln Gln Tyr Val Glu Thr His Gln Asn 
    1250                1255                1260                
Glu Phe Lys Glu Leu Leu Thr Thr Ile Ile Ser Leu Ser Lys Lys Tyr 
1265                1270                1275                1280
Ile Gln Lys Pro Asn Val Glu Ser Leu Leu Gln Gln Ala Phe Glu Gln 
                1285                1290                1295    
Asn Ala Asp Lys Asp Ile Tyr Gln Leu Ser Glu Ser Phe Ile Ser Leu 
            1300                1305                1310        
Leu Lys Leu Thr Ser Phe Gly Ala Pro Gly Ala Phe Lys Phe Leu Gly 
        1315                1320                1325            
Val Glu Ile Ser Gln Ser Arg Tyr Lys Ser Val Ser Gln Phe Leu Asn 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Lys Leu Gly Glu Asp 
                1365            

<210> 237
<211> 104
<212> DNA
<213> Artificial Sequence


<220> 
<223> gRNA1

<400> 237
gtcaggagac taggaaggag ggttttagag ctagaaatag caagttaaaa taaggctagt     60

ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttt                     104


<210> 238
<211> 103
<212> DNA
<213> Artificial Sequence


<220> 
<223> gRNA2

<400> 238
gttaatgtgg ctctggttct gttttagagc tagaaatagc aagttaaaat aaggctagtc     60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                      103


<210> 239
<211> 103
<212> DNA
<213> Artificial Sequence


<220> 
<223> gRNA3

<400> 239
ggggccacta gggacaggat gttttagagc tagaaatagc aagttaaaat aaggctagtc     60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                      103


<210> 240
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> P5_2

<400> 240
aatgatacgg cgaccaccga gatctacac                                      29


<210> 241
<211> 65
<212> DNA
<213> Artificial Sequence


<220> 
<223> P705

<400> 241
caagcagaag acggcatacg agataggagt ccgtgactgg agtcctctct atgggcagtc     60

ggtga                                                                 65


<210> 242
<211> 65
<212> DNA
<213> Artificial Sequence


<220> 
<223> P706

<400> 242
caagcagaag acggcatacg agatcatgcc tagtgactgg agtcctctct atgggcagtc     60

ggtga                                                                 65


<210> 243
<211> 65
<212> DNA
<213> Artificial Sequence


<220> 
<223> P707

<400> 243
caagcagaag acggcatacg agatgtagag aggtgactgg agtcctctct atgggcagtc     60

ggtga                                                                 65


<210> 244
<211> 65
<212> DNA
<213> Artificial Sequence


<220> 
<223> P708

<400> 244
caagcagaag acggcatacg agatcctctc tggtgactgg agtcctctct atgggcagtc     60

ggtga                                                                 65


<210> 245
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Index1

<400> 245
atcaccgact gcccatagag aggactccag tcac                                 34


<210> 246
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Read2

<400> 246
gtgactggag tcctctctat gggcagtcgg tgat                                 34


