               SEQUENCE LISTING

<110> ENZA ZADEN BEHEER B.V.

<120> MELON HAVING INCREASED SUCROSE CONTENT

<130> P182640PC00

<160> 8

<170> BiSSAP 1.3.6

<210> 1
<211> 630
<212> PRT
<213> Cucumis melo


<220> 
<223> AIN2 protein

<400> 1
Met Ala Ser Thr Ser Ser Asp Leu Ile Pro Pro Pro Tyr Ser Ala Leu 
1               5                   10                  15      
Pro Asp Arg His Pro Ser Pro Ala Leu Pro Arg Lys Ser Arg Asn Phe 
            20                  25                  30          
Thr Thr Thr Thr Ile Leu Ile Ser Phe Leu Leu Leu Val Ser Leu Leu 
        35                  40                  45              
Ala Leu Phe Ser Tyr Thr Asn Thr Ser Ser Pro Glu Ser Val Lys Leu 
    50                  55                  60                  
Pro Glu Asn Thr Gln Ser Arg Leu Ala Arg Gly Val Ala Glu Gly Val 
65                  70                  75                  80  
Ser Ala Lys Ser Asn Pro Tyr Phe Ser Asp Ser Val Asp Gly Ser Phe 
                85                  90                  95      
Asn Trp Thr Asn Ala Met Phe Ser Trp Gln Arg Thr Ala Phe His Phe 
            100                 105                 110         
Gln Pro Glu Gly Asn Trp Met Asn Asp Pro Asn Gly Pro Leu Tyr His 
        115                 120                 125             
Lys Gly Trp Tyr His Leu Phe Tyr Gln Tyr Asn Pro Glu Ser Ala Val 
    130                 135                 140                 
Trp Gly Asn Ile Ser Trp Gly His Ala Val Ser Arg Asp Leu Ile His 
145                 150                 155                 160 
Trp Leu Tyr Leu Pro Tyr Ala Met Val Pro Asp Gln Pro Tyr Asp Val 
                165                 170                 175     
Asn Gly Val Trp Thr Gly Ser Ala Thr Ile Leu Pro Asp Gly Arg Ile 
            180                 185                 190         
Ile Met Leu Tyr Thr Gly Asp Thr Ile Asp Gly Val Gln Val Gln Asn 
        195                 200                 205             
Leu Ala Tyr Pro Ala Asn Leu Thr Asp Pro Leu Leu Leu Asn Trp Val 
    210                 215                 220                 
Lys Tyr Pro Gly Asn Pro Val Leu Val Pro Pro Pro Gly Ile Gly Leu 
225                 230                 235                 240 
Lys Asp Phe Arg Asp Pro Thr Thr Ala Trp Leu Gly Pro Asp Gly Lys 
                245                 250                 255     
Trp Arg Ile Thr Ile Gly Ser Arg Val Gly Thr Thr Leu Gly Val Ser 
            260                 265                 270         
Leu Val Tyr Thr Thr Asn Asp Phe Ile Lys Tyr Glu Leu Val Asp Arg 
        275                 280                 285             
Phe Leu His Ala Val Pro Gly Thr Gly Met Trp Glu Cys Val Asp Phe 
    290                 295                 300                 
Tyr Pro Val Ser Val Asp Gly Ser Lys Gly Leu Asp Thr Ser Glu Asn 
305                 310                 315                 320 
Gly Gly Gly Val Lys His Val Leu Lys Ala Ser Leu Asp Asp Thr Lys 
                325                 330                 335     
Met Asp His Tyr Ala Ile Gly Thr Tyr Phe Ala Asn Asn Asp Thr Trp 
            340                 345                 350         
Val Pro Asp Asn Pro Glu Glu Asp Val Gly Ile Gly Leu Lys Tyr Asp 
        355                 360                 365             
Tyr Gly Arg Tyr Tyr Ala Ser Lys Thr Phe Tyr Asp Gln Asn Lys Glu 
    370                 375                 380                 
Arg Arg Ile Leu Trp Gly Trp Ile Asn Glu Thr Asp Thr Glu Ala Asn 
385                 390                 395                 400 
Asp Leu Ala Lys Gly Trp Ala Ser Val Gln Thr Val Pro Arg Thr Val 
                405                 410                 415     
Leu Phe Asp Gln Lys Thr Gly Ser Asn Ile Ile Gln Trp Pro Val Glu 
            420                 425                 430         
Glu Val Glu Ser Leu Arg Leu Gly Ser Asn Glu Phe Asn Asp Val Leu 
        435                 440                 445             
Leu Val Pro Gly Ser Val Val Glu Leu Glu Val Gly Pro Ala Thr Gln 
    450                 455                 460                 
Leu Asp Ile Leu Ala Glu Phe Glu Val Glu Val Leu Gly Ser Glu Asn 
465                 470                 475                 480 
Gly Thr Val Ser Glu Glu Gly Cys Gly Gly Gly Ala Ala Glu Arg Ser 
                485                 490                 495     
Ser Leu Gly Pro Phe Gly Val Leu Val Leu Ala His Gln Ser Leu Ser 
            500                 505                 510         
Glu Phe Thr Pro Ile Tyr Phe Asn Val Ala Asn Ser Ser Lys Gly Ser 
        515                 520                 525             
Gly Glu Ala Tyr Phe Cys Ala Asp Glu Thr Arg Ser Ser Lys Ala Pro 
    530                 535                 540                 
Asp Val Phe Lys Gln Val Tyr Gly Ser Lys Ile Pro Val Leu Glu Gly 
545                 550                 555                 560 
Glu Asn Tyr Ser Met Arg Val Leu Val Asp His Ser Ile Val Glu Ser 
                565                 570                 575     
Phe Gly Gln Gly Gly Arg Arg Val Ile Thr Ser Arg Ile Tyr Pro Thr 
            580                 585                 590         
Glu Ala Ile Tyr Gly Ala Ala Lys Leu Phe Leu Phe Asn Asn Gly Thr 
        595                 600                 605             
Ser Ala Asn Val Lys Ala Thr Val Lys Val Trp Gln Leu Asn Ser Ala 
    610                 615                 620                 
Phe Ile Gln Pro Tyr Phe 
625                 630 

<210> 2
<211> 1893
<212> DNA
<213> Cucumis melo


<220> 
<223> AIN2 coding sequence

<400> 2
atggcttcta cttcttcaga tctcatccct ccgccttact ccgccttgcc ggaccgccat      60

ccctccccgg cgcttccccg aaaatcccgc aacttcacta caactaccat tctcatctct     120

tttctccttc tcgtttcttt acttgctctg ttttcttaca ctaacacctc atctcccgaa     180

tccgtcaagt tgccggaaaa cacccaatcg cgactagcga gaggagtcgc tgagggcgtt     240

tccgccaaat cgaaccctta tttttccgat tctgttgatg gttctttcaa ttggaccaac     300

gctatgttct cttggcaacg aactgccttc cattttcaac ctgaaggcaa ttggatgaac     360

gatcccaatg ggccattata tcacaagggt tggtatcatc tattttacca atataaccct     420

gaatccgctg tatggggcaa cataagttgg ggccacgccg tttcaagaga tctcatccat     480

tggctctatc tcccttacgc catggtccca gatcaaccgt acgatgtcaa cggcgtttgg     540

accgggtccg ccacaatcct tccggacggt cggatcataa tgctctacac tggcgatacc     600

attgatggag tgcaggtcca aaatctagca taccctgcca acctaaccga tcccctcctc     660

ttaaattggg ttaagtaccc gggcaacccg gtcttggttc ctccacccgg cattggcctc     720

aaagacttcc gcgaccccac tacggcctgg ctcggccccg acggtaagtg gcggatcacc     780

attgggtcac gcgtggggac aacattggga gtttcgttgg tttatactac taatgatttt     840

attaagtacg agcttgtgga ccgattcttg cacgcagtcc cgggtacggg catgtgggaa     900

tgcgttgatt tttatccggt atcggttgac gggtcgaagg gtttggatac gtcggagaat     960

ggtggtggtg taaagcacgt gctcaaggcg agtttggatg atactaaaat ggatcattat    1020

gcaattggga cttattttgc taataatgat acttgggttc ccgataaccc ggaagaagat    1080

gtgggaattg gcttgaaata tgattatgga agatactatg cttccaagac attttacgac    1140

caaaataaag agaggaggat cttgtggggt tggatcaatg aaactgacac tgaagctaat    1200

gatttggcaa aaggctgggc ctccgttcag acggttccga ggacagtgct attcgatcag    1260

aagacaggca gcaacataat ccaatggcct gtggaagagg tggaaagttt gagactgggg    1320

agcaatgaat ttaatgacgt gctcctcgta cctggctctg tcgtggaact cgaagtcggc    1380

cctgctacac agttggatat attggcggag tttgaagtgg aagtgctcgg gtcagagaat    1440

ggtaccgtct ccgaggaagg ctgtggtggg ggtgcggcgg agagaagtag tttaggacca    1500

tttggtgtgt tggttttggc tcatcaatcg ctttccgagt ttactcctat ttacttcaat    1560

gttgctaatt caagtaaagg cagtggggaa gcttactttt gtgccgacga gacaaggtca    1620

tcaaaagctc ctgatgtttt caaacaagtt tatggaagca aaattcctgt tttagagggt    1680

gaaaattact ccatgagagt gttggtggat cactcaattg tggagagttt tggacaaggt    1740

gggagaagag tgataacatc acgaatttat ccaacagaag caatttatgg tgcagccaag    1800

cttttccttt tcaacaatgg cacatcagca aatgtaaagg ccactgtcaa agtatggcag    1860

ctcaattctg ccttcattca accatatttt tga                                 1893


<210> 3
<211> 630
<212> PRT
<213> Cucumis melo


<220> 
<223> AIN2 mutant protein

<400> 3
Met Ala Ser Thr Ser Ser Asp Leu Ile Pro Pro Pro Tyr Ser Ala Leu 
1               5                   10                  15      
Pro Asp Arg His Pro Ser Pro Ala Leu Pro Arg Lys Ser Arg Asn Phe 
            20                  25                  30          
Thr Thr Thr Thr Ile Leu Ile Ser Phe Leu Leu Leu Val Ser Leu Leu 
        35                  40                  45              
Ala Leu Phe Ser Tyr Thr Asn Thr Ser Ser Pro Glu Ser Val Lys Leu 
    50                  55                  60                  
Pro Glu Asn Thr Gln Ser Arg Leu Ala Arg Gly Val Ala Glu Gly Val 
65                  70                  75                  80  
Ser Ala Lys Ser Asn Pro Tyr Phe Ser Asp Ser Val Asp Gly Ser Phe 
                85                  90                  95      
Asn Trp Thr Asn Ala Met Phe Ser Trp Gln Arg Thr Ala Phe His Phe 
            100                 105                 110         
Gln Pro Glu Gly Asn Trp Met Asn Asp Pro Asn Gly Pro Leu Tyr His 
        115                 120                 125             
Lys Gly Trp Tyr His Leu Phe Tyr Gln Tyr Asn Pro Glu Ser Ala Val 
    130                 135                 140                 
Trp Gly Asn Ile Ser Trp Gly His Ala Val Ser Arg Asp Leu Ile His 
145                 150                 155                 160 
Trp Leu Tyr Leu Pro Tyr Ala Met Val Pro Asp Gln Pro Tyr Asp Val 
                165                 170                 175     
Asn Gly Val Trp Thr Gly Ser Ala Thr Ile Leu Pro Asp Gly Arg Ile 
            180                 185                 190         
Ile Met Leu Tyr Thr Gly Asp Thr Ile Asp Gly Val Gln Val Gln Asn 
        195                 200                 205             
Leu Ala Tyr Pro Ala Asn Leu Thr Asp Pro Leu Leu Leu Asn Trp Val 
    210                 215                 220                 
Lys Tyr Pro Gly Asn Pro Val Leu Val Pro Pro Pro Gly Ile Gly Leu 
225                 230                 235                 240 
Lys Asp Phe Arg Asp Pro Thr Thr Ala Trp Leu Gly Pro Asp Gly Lys 
                245                 250                 255     
Trp Arg Ile Thr Ile Gly Ser Arg Val Gly Thr Thr Leu Gly Val Ser 
            260                 265                 270         
Leu Val Tyr Thr Thr Asn Asp Phe Ile Lys Tyr Glu Leu Val Asp Arg 
        275                 280                 285             
Phe Leu His Ala Val Pro Gly Thr Gly Met Trp Glu Cys Val Asp Phe 
    290                 295                 300                 
Tyr Pro Val Ser Val Asp Gly Ser Lys Gly Leu Asp Thr Ser Glu Asn 
305                 310                 315                 320 
Gly Gly Gly Val Lys His Val Leu Lys Ala Ser Leu Asp Asp Thr Lys 
                325                 330                 335     
Met Asp His Tyr Ala Ile Gly Thr Tyr Phe Ala Asn Asn Asp Thr Trp 
            340                 345                 350         
Val Pro Asp Asn Pro Glu Glu Asp Val Gly Ile Gly Leu Lys Tyr Asp 
        355                 360                 365             
Tyr Gly Arg Tyr Tyr Ala Ser Lys Ile Phe Tyr Asp Gln Asn Lys Glu 
    370                 375                 380                 
Arg Arg Ile Leu Trp Gly Trp Ile Asn Glu Thr Asp Thr Glu Ala Asn 
385                 390                 395                 400 
Asp Leu Ala Lys Gly Trp Ala Ser Val Gln Thr Val Pro Arg Thr Val 
                405                 410                 415     
Leu Phe Asp Gln Lys Thr Gly Ser Asn Ile Ile Gln Trp Pro Val Glu 
            420                 425                 430         
Glu Val Glu Ser Leu Arg Leu Gly Ser Asn Glu Phe Asn Asp Val Leu 
        435                 440                 445             
Leu Val Pro Gly Ser Val Val Glu Leu Glu Val Gly Pro Ala Thr Gln 
    450                 455                 460                 
Leu Asp Ile Leu Ala Glu Phe Glu Val Glu Val Leu Gly Ser Glu Asn 
465                 470                 475                 480 
Gly Thr Val Ser Glu Glu Gly Cys Gly Gly Gly Ala Ala Glu Arg Ser 
                485                 490                 495     
Ser Leu Gly Pro Phe Gly Val Leu Val Leu Ala His Gln Ser Leu Ser 
            500                 505                 510         
Glu Phe Thr Pro Ile Tyr Phe Asn Val Ala Asn Ser Ser Lys Gly Ser 
        515                 520                 525             
Gly Glu Ala Tyr Phe Cys Ala Asp Glu Thr Arg Ser Ser Lys Ala Pro 
    530                 535                 540                 
Asp Val Phe Lys Gln Val Tyr Gly Ser Lys Ile Pro Val Leu Glu Gly 
545                 550                 555                 560 
Glu Asn Tyr Ser Met Arg Val Leu Val Asp His Ser Ile Val Glu Ser 
                565                 570                 575     
Phe Gly Gln Gly Gly Arg Arg Val Ile Thr Ser Arg Ile Tyr Pro Thr 
            580                 585                 590         
Glu Ala Ile Tyr Gly Ala Ala Lys Leu Phe Leu Phe Asn Asn Gly Thr 
        595                 600                 605             
Ser Ala Asn Val Lys Ala Thr Val Lys Val Trp Gln Leu Asn Ser Ala 
    610                 615                 620                 
Phe Ile Gln Pro Tyr Phe 
625                 630 

<210> 4
<211> 1893
<212> DNA
<213> Cucumis melo


<220> 
<223> AIN2 mutant coding sequence

<400> 4
atggcttcta cttcttcaga tctcatccct ccgccttact ccgccttgcc ggaccgccat      60

ccctccccgg cgcttccccg aaaatcccgc aacttcacta caactaccat tctcatctct     120

tttctccttc tcgtttcttt acttgctctg ttttcttaca ctaacacctc atctcccgaa     180

tccgtcaagt tgccggaaaa cacccaatcg cgactagcga gaggagtcgc tgagggcgtt     240

tccgccaaat cgaaccctta tttttccgat tctgttgatg gttctttcaa ttggaccaac     300

gctatgttct cttggcaacg aactgccttc cattttcaac ctgaaggcaa ttggatgaac     360

gatcccaatg ggccattata tcacaagggt tggtatcatc tattttacca atataaccct     420

gaatccgctg tatggggcaa cataagttgg ggccacgccg tttcaagaga tctcatccat     480

tggctctatc tcccttacgc catggtccca gatcaaccgt acgatgtcaa cggcgtttgg     540

accgggtccg ccacaatcct tccggacggt cggatcataa tgctctacac tggcgatacc     600

attgatggag tgcaggtcca aaatctagca taccctgcca acctaaccga tcccctcctc     660

ttaaattggg ttaagtaccc gggcaacccg gtcttggttc ctccacccgg cattggcctc     720

aaagacttcc gcgaccccac tacggcctgg ctcggccccg acggtaagtg gcggatcacc     780

attgggtcac gcgtggggac aacattggga gtttcgttgg tttatactac taatgatttt     840

attaagtacg agcttgtgga ccgattcttg cacgcagtcc cgggtacggg catgtgggaa     900

tgcgttgatt tttatccggt atcggttgac gggtcgaagg gtttggatac gtcggagaat     960

ggtggtggtg taaagcacgt gctcaaggcg agtttggatg atactaaaat ggatcattat    1020

gcaattggga cttattttgc taataatgat acttgggttc ccgataaccc ggaagaagat    1080

gtgggaattg gcttgaaata tgattatgga agatactatg cttccaagat attttacgac    1140

caaaataaag agaggaggat cttgtggggt tggatcaatg aaactgacac tgaagctaat    1200

gatttggcaa aaggctgggc ctccgttcag acggttccga ggacagtgct attcgatcag    1260

aagacaggca gcaacataat ccaatggcct gtggaagagg tggaaagttt gagactgggg    1320

agcaatgaat ttaatgacgt gctcctcgta cctggctctg tcgtggaact cgaagtcggc    1380

cctgctacac agttggatat attggcggag tttgaagtgg aagtgctcgg gtcagagaat    1440

ggtaccgtct ccgaggaagg ctgtggtggg ggtgcggcgg agagaagtag tttaggacca    1500

tttggtgtgt tggttttggc tcatcaatcg ctttccgagt ttactcctat ttacttcaat    1560

gttgctaatt caagtaaagg cagtggggaa gcttactttt gtgccgacga gacaaggtca    1620

tcaaaagctc ctgatgtttt caaacaagtt tatggaagca aaattcctgt tttagagggt    1680

gaaaattact ccatgagagt gttggtggat cactcaattg tggagagttt tggacaaggt    1740

gggagaagag tgataacatc acgaatttat ccaacagaag caatttatgg tgcagccaag    1800

cttttccttt tcaacaatgg cacatcagca aatgtaaagg ccactgtcaa agtatggcag    1860

ctcaattctg ccttcattca accatatttt tga                                 1893


<210> 5
<211> 626
<212> PRT
<213> Citrullus lanatus


<220> 
<223> AIN2 protein

<400> 5
Met Asp Ser Ala Ser Asp Leu Thr Pro Thr Pro Tyr Thr Pro Leu Pro 
1               5                   10                  15      
Asp His His Leu Ser Pro Pro Leu Pro Arg Lys Ser Leu Asn Phe Thr 
            20                  25                  30          
Thr Ala Thr Ile Leu Ile Ser Leu Leu Leu Leu Leu Val Ser Leu Leu 
        35                  40                  45              
Thr Leu Leu Ser Tyr His Thr Pro Ser Pro His Ala Thr Gln Ser Pro 
    50                  55                  60                  
Pro Pro Phe Arg Leu Ala Arg Gly Val Ala Glu Gly Val Ser Ala Lys 
65                  70                  75                  80  
Ser Asn Pro Ser Phe Ser Asp Ser Val His Ser Phe Asn Trp Thr Asn 
                85                  90                  95      
Ala Met Phe Ser Trp Gln Arg Thr Ala Phe His Phe Gln Pro Glu Gly 
            100                 105                 110         
Asn Trp Met Asn Asp Pro Asn Gly Pro Leu Tyr His Lys Gly Trp Tyr 
        115                 120                 125             
His Leu Phe Tyr Gln Tyr Asn Pro Glu Ser Ala Val Trp Gly Asn Ile 
    130                 135                 140                 
Ser Trp Gly His Ala Val Ser Arg Asp Leu Ile His Trp Leu Tyr Leu 
145                 150                 155                 160 
Pro Tyr Ala Met Val Pro Asp Gln Pro Tyr Asp Val Asn Gly Val Trp 
                165                 170                 175     
Thr Gly Ser Ala Thr Ile Leu Pro Asp Gly Arg Ile Ile Met Leu Tyr 
            180                 185                 190         
Thr Gly Asp Thr Ile Asp Gly Val Gln Val Gln Asn Leu Ala Tyr Pro 
        195                 200                 205             
Ala Asn Leu Ser Asp Pro Leu Leu Leu Asn Trp Val Lys Tyr Pro Gly 
    210                 215                 220                 
Asn Pro Val Leu Val Pro Pro Pro Gly Ile Gly Pro Lys Asp Phe Arg 
225                 230                 235                 240 
Asp Pro Thr Thr Ala Trp Leu Gly His Asp Gly Lys Trp Arg Ile Thr 
                245                 250                 255     
Ile Gly Ser Arg Val Gly Thr Thr Leu Gly Val Ser Leu Val Tyr Thr 
            260                 265                 270         
Thr Asn Asp Phe Ile Lys Tyr Glu Leu Val Asp Arg Phe Leu His Ala 
        275                 280                 285             
Val Pro Gly Thr Gly Met Trp Glu Cys Val Asp Phe Tyr Pro Ile Ser 
    290                 295                 300                 
Val Gln Gly Ser Lys Gly Leu Asp Thr Ser Ser Asn Gly Gly Gly Ile 
305                 310                 315                 320 
Lys His Val Leu Lys Ala Ser Leu Asp Asp Thr Lys Met Asp His Tyr 
                325                 330                 335     
Ala Ile Gly Thr Tyr Phe Gly Asn Asn Asp Thr Trp Val Pro Asp Asn 
            340                 345                 350         
Pro Glu Glu Asp Val Gly Ile Gly Leu Lys Leu Asp Tyr Gly Arg Tyr 
        355                 360                 365             
Tyr Ala Ser Lys Thr Phe Tyr Asp Gln His Lys Glu Arg Arg Ile Leu 
    370                 375                 380                 
Trp Gly Trp Ile Asn Glu Thr Asp Thr Glu Ala Asn Asp Leu Ala Lys 
385                 390                 395                 400 
Gly Trp Ala Ser Val Gln Thr Val Pro Arg Thr Val Leu Phe Asp Gln 
                405                 410                 415     
Lys Thr Gly Ser Asn Ile Ile Gln Trp Pro Val Glu Glu Val Glu Ser 
            420                 425                 430         
Leu Arg Leu Gly Ser Thr Glu Phe Asn Gly Val Leu Val Glu Pro Gly 
        435                 440                 445             
Ser Val Val Glu Leu Glu Val Gly Thr Ala Thr Gln Leu Asp Ile Leu 
    450                 455                 460                 
Ala Glu Phe Glu Leu Glu Ala Ile Gly Ser Glu Asn Ala Thr Val Ser 
465                 470                 475                 480 
Glu Glu Gly Cys Gly Asp Gly Ala Ala Glu Arg Ser Ser Leu Gly Pro 
                485                 490                 495     
Phe Gly Leu Leu Val Leu Ala His Gln Ser Leu Ser Glu Leu Thr Pro 
            500                 505                 510         
Ile Tyr Phe Asn Val Ala Asn Ser Ser Lys Gly Ser Arg Glu Ala Tyr 
        515                 520                 525             
Phe Cys Ala Asp Glu Lys Arg Ser Ser Lys Ala Pro Asp Val Phe Lys 
    530                 535                 540                 
Gln Val Tyr Gly Ser Lys Ile Pro Val Leu Glu Gly Glu Asn Phe Ser 
545                 550                 555                 560 
Met Arg Val Leu Val Asp His Ser Ile Val Glu Ser Phe Gly Gln Gly 
                565                 570                 575     
Gly Arg Arg Val Ile Thr Ser Arg Ile Tyr Pro Thr Glu Ala Ile Tyr 
            580                 585                 590         
Gly Ala Ala Lys Leu Phe Leu Phe Asn Asn Ala Thr Ser Ala Asn Val 
        595                 600                 605             
Lys Ala Thr Val Lys Val Trp Gln Leu Asn Ser Ala Phe Ile Gln Pro 
    610                 615                 620                 
Phe Ser 
625     

<210> 6
<211> 1881
<212> DNA
<213> Citrullus lanatus


<220> 
<223> AIN2 coding sequence

<400> 6
atggattcag cttcagatct cacccctacg ccctacacgc ccttgccgga ccaccatctc      60

tctcccccac ttccccggaa atccctcaac ttcactactg ctaccattct catctctctt     120

cttcttcttc ttgtctctct acttactctg ctttcatacc acaccccatc tccccatgcc     180

acccaatctc cgccgccgtt taggctcgct agaggagttg ccgagggcgt ctccgccaaa     240

tccaaccctt ccttttccga ttccgtccat tctttcaatt ggactaacgc tatgttctct     300

tggcaacgaa ctgccttcca ttttcaacct gaaggcaatt ggatgaacga tcccaatggc     360

ccactgtacc acaagggttg gtatcatctc ttttatcaat acaaccctga atcagccgtg     420

tggggcaaca taagttgggg ccacgccgtc tcaagagatc tcatccattg gttatatctc     480

ccctacgcca tggtccctga tcaaccctac gatgtcaatg gcgtatggac cggatccgcc     540

acaattcttc ctgacggtag gatcataatg ctctatacag gcgataccat tgatggagtg     600

caagtccaaa atcttgcata ccctgccaac ctatccgatc ccctcctttt aaattgggtc     660

aagtacccgg gcaacccggt cttggttccc ccacccggca ttggcccaaa agactttcgg     720

gaccccacta ccgcatggct tggccacgac ggtaagtggc ggatcaccat cgggtcacgc     780

gttgggacga cattgggcgt ttcgttggtt tatactacta atgattttat taagtatgag     840

cttgtggatc ggttcttaca tgctgtcccg ggtacgggta tgtgggaatg tgtcgatttt     900

tatccgatat cggtccaggg gtcgaagggt ttggacacgt cgtcaaatgg aggtggaatt     960

aagcacgtgc ttaaggcaag tttggatgat actaaaatgg atcattatgc aattggaact    1020

tattttggta ataatgatac ttgggtaccc gataacccgg aagaggatgt gggaattggg    1080

ttgaaattgg attatggaag gtattatgct tccaagacat tttatgacca acataaagag    1140

aggagaatct tgtggggttg gattaatgaa actgacactg aagctaatga cttggcaaaa    1200

ggctgggcct ctgtacagac tgttccgagg acagtgctat tcgaccagaa gaccggcagc    1260

aacataatcc aatggcctgt ggaagaggtg gagagtttga gattggggag cactgaattt    1320

aatggcgtgc tcgtcgaacc tggctccgtc gtcgaactag aagtcggcac tgctacacaa    1380

ttggacatat tggcggagtt tgaactggaa gcgatcgggt cggagaatgc taccgtctcc    1440

gaggaaggct gtggcgacgg tgcagcagag agaagtagtt taggaccatt tgggctgttg    1500

gttctggctc atcaatcact ttccgagctt actcctattt atttcaatgt tgccaattca    1560

agtaaaggca gtcgggaggc ttacttctgt gccgacgaga aaagatcatc aaaggctcca    1620

gatgttttca aacaagttta tggaagcaaa attcctgttt tagagggtga aaatttctcc    1680

atgagagtgt tggtggatca ctcaattgtg gagagttttg gacaaggtgg gagaagagta    1740

ataacatcac gcatttatcc aacagaagca atatatggag cagcaaagct tttccttttc    1800

aacaatgcta catcagcaaa tgtgaaggca actgtgaaag tatggcaact caattctgct    1860

ttcattcaac cattttcctg a                                              1881


<210> 7
<211> 626
<212> PRT
<213> Citrullus lanatus


<220> 
<223> AIN2 mutant protein

<400> 7
Met Asp Ser Ala Ser Asp Leu Thr Pro Thr Pro Tyr Thr Pro Leu Pro 
1               5                   10                  15      
Asp His His Leu Ser Pro Pro Leu Pro Arg Lys Ser Leu Asn Phe Thr 
            20                  25                  30          
Thr Ala Thr Ile Leu Ile Ser Leu Leu Leu Leu Leu Val Ser Leu Leu 
        35                  40                  45              
Thr Leu Leu Ser Tyr His Thr Pro Ser Pro His Ala Thr Gln Ser Pro 
    50                  55                  60                  
Pro Pro Phe Arg Leu Ala Arg Gly Val Ala Glu Gly Val Ser Ala Lys 
65                  70                  75                  80  
Ser Asn Pro Ser Phe Ser Asp Ser Val His Ser Phe Asn Trp Thr Asn 
                85                  90                  95      
Ala Met Phe Ser Trp Gln Arg Thr Ala Phe His Phe Gln Pro Glu Gly 
            100                 105                 110         
Asn Trp Met Asn Asp Pro Asn Gly Pro Leu Tyr His Lys Gly Trp Tyr 
        115                 120                 125             
His Leu Phe Tyr Gln Tyr Asn Pro Glu Ser Ala Val Trp Gly Asn Ile 
    130                 135                 140                 
Ser Trp Gly His Ala Val Ser Arg Asp Leu Ile His Trp Leu Tyr Leu 
145                 150                 155                 160 
Pro Tyr Ala Met Val Pro Asp Gln Pro Tyr Asp Val Asn Gly Val Trp 
                165                 170                 175     
Thr Gly Ser Ala Thr Ile Leu Pro Asp Gly Arg Ile Ile Met Leu Tyr 
            180                 185                 190         
Thr Gly Asp Thr Ile Asp Gly Val Gln Val Gln Asn Leu Ala Tyr Pro 
        195                 200                 205             
Ala Asn Leu Ser Asp Pro Leu Leu Leu Asn Trp Val Lys Tyr Pro Gly 
    210                 215                 220                 
Asn Pro Val Leu Val Pro Pro Pro Gly Ile Gly Pro Lys Asp Phe Arg 
225                 230                 235                 240 
Asp Pro Thr Thr Ala Trp Leu Gly His Asp Gly Lys Trp Arg Ile Thr 
                245                 250                 255     
Ile Gly Ser Arg Val Gly Thr Thr Leu Gly Val Ser Leu Val Tyr Thr 
            260                 265                 270         
Thr Asn Asp Phe Ile Lys Tyr Glu Leu Val Asp Arg Phe Leu His Ala 
        275                 280                 285             
Val Pro Gly Thr Gly Met Trp Glu Cys Val Asp Phe Tyr Pro Ile Ser 
    290                 295                 300                 
Val Gln Gly Ser Lys Gly Leu Asp Thr Ser Ser Asn Gly Gly Gly Ile 
305                 310                 315                 320 
Lys His Val Leu Lys Ala Ser Leu Asp Asp Thr Lys Met Asp His Tyr 
                325                 330                 335     
Ala Ile Gly Thr Tyr Phe Gly Asn Asn Asp Thr Trp Val Pro Asp Asn 
            340                 345                 350         
Pro Glu Glu Asp Val Gly Ile Gly Leu Lys Leu Asp Tyr Gly Arg Tyr 
        355                 360                 365             
Tyr Ala Ser Lys Ile Phe Tyr Asp Gln His Lys Glu Arg Arg Ile Leu 
    370                 375                 380                 
Trp Gly Trp Ile Asn Glu Thr Asp Thr Glu Ala Asn Asp Leu Ala Lys 
385                 390                 395                 400 
Gly Trp Ala Ser Val Gln Thr Val Pro Arg Thr Val Leu Phe Asp Gln 
                405                 410                 415     
Lys Thr Gly Ser Asn Ile Ile Gln Trp Pro Val Glu Glu Val Glu Ser 
            420                 425                 430         
Leu Arg Leu Gly Ser Thr Glu Phe Asn Gly Val Leu Val Glu Pro Gly 
        435                 440                 445             
Ser Val Val Glu Leu Glu Val Gly Thr Ala Thr Gln Leu Asp Ile Leu 
    450                 455                 460                 
Ala Glu Phe Glu Leu Glu Ala Ile Gly Ser Glu Asn Ala Thr Val Ser 
465                 470                 475                 480 
Glu Glu Gly Cys Gly Asp Gly Ala Ala Glu Arg Ser Ser Leu Gly Pro 
                485                 490                 495     
Phe Gly Leu Leu Val Leu Ala His Gln Ser Leu Ser Glu Leu Thr Pro 
            500                 505                 510         
Ile Tyr Phe Asn Val Ala Asn Ser Ser Lys Gly Ser Arg Glu Ala Tyr 
        515                 520                 525             
Phe Cys Ala Asp Glu Lys Arg Ser Ser Lys Ala Pro Asp Val Phe Lys 
    530                 535                 540                 
Gln Val Tyr Gly Ser Lys Ile Pro Val Leu Glu Gly Glu Asn Phe Ser 
545                 550                 555                 560 
Met Arg Val Leu Val Asp His Ser Ile Val Glu Ser Phe Gly Gln Gly 
                565                 570                 575     
Gly Arg Arg Val Ile Thr Ser Arg Ile Tyr Pro Thr Glu Ala Ile Tyr 
            580                 585                 590         
Gly Ala Ala Lys Leu Phe Leu Phe Asn Asn Ala Thr Ser Ala Asn Val 
        595                 600                 605             
Lys Ala Thr Val Lys Val Trp Gln Leu Asn Ser Ala Phe Ile Gln Pro 
    610                 615                 620                 
Phe Ser 
625     

<210> 8
<211> 1881
<212> DNA
<213> Citrullus lanatus


<220> 
<223> AIN2 mutant coding sequence

<400> 8
atggattcag cttcagatct cacccctacg ccctacacgc ccttgccgga ccaccatctc      60

tctcccccac ttccccggaa atccctcaac ttcactactg ctaccattct catctctctt     120

cttcttcttc ttgtctctct acttactctg ctttcatacc acaccccatc tccccatgcc     180

acccaatctc cgccgccgtt taggctcgct agaggagttg ccgagggcgt ctccgccaaa     240

tccaaccctt ccttttccga ttccgtccat tctttcaatt ggactaacgc tatgttctct     300

tggcaacgaa ctgccttcca ttttcaacct gaaggcaatt ggatgaacga tcccaatggc     360

ccactgtacc acaagggttg gtatcatctc ttttatcaat acaaccctga atcagccgtg     420

tggggcaaca taagttgggg ccacgccgtc tcaagagatc tcatccattg gttatatctc     480

ccctacgcca tggtccctga tcaaccctac gatgtcaatg gcgtatggac cggatccgcc     540

acaattcttc ctgacggtag gatcataatg ctctatacag gcgataccat tgatggagtg     600

caagtccaaa atcttgcata ccctgccaac ctatccgatc ccctcctttt aaattgggtc     660

aagtacccgg gcaacccggt cttggttccc ccacccggca ttggcccaaa agactttcgg     720

gaccccacta ccgcatggct tggccacgac ggtaagtggc ggatcaccat cgggtcacgc     780

gttgggacga cattgggcgt ttcgttggtt tatactacta atgattttat taagtatgag     840

cttgtggatc ggttcttaca tgctgtcccg ggtacgggta tgtgggaatg tgtcgatttt     900

tatccgatat cggtccaggg gtcgaagggt ttggacacgt cgtcaaatgg aggtggaatt     960

aagcacgtgc ttaaggcaag tttggatgat actaaaatgg atcattatgc aattggaact    1020

tattttggta ataatgatac ttgggtaccc gataacccgg aagaggatgt gggaattggg    1080

ttgaaattgg attatggaag gtattatgct tccaagatat tttatgacca acataaagag    1140

aggagaatct tgtggggttg gattaatgaa actgacactg aagctaatga cttggcaaaa    1200

ggctgggcct ctgtacagac tgttccgagg acagtgctat tcgaccagaa gaccggcagc    1260

aacataatcc aatggcctgt ggaagaggtg gagagtttga gattggggag cactgaattt    1320

aatggcgtgc tcgtcgaacc tggctccgtc gtcgaactag aagtcggcac tgctacacaa    1380

ttggacatat tggcggagtt tgaactggaa gcgatcgggt cggagaatgc taccgtctcc    1440

gaggaaggct gtggcgacgg tgcagcagag agaagtagtt taggaccatt tgggctgttg    1500

gttctggctc atcaatcact ttccgagctt actcctattt atttcaatgt tgccaattca    1560

agtaaaggca gtcgggaggc ttacttctgt gccgacgaga aaagatcatc aaaggctcca    1620

gatgttttca aacaagttta tggaagcaaa attcctgttt tagagggtga aaatttctcc    1680

atgagagtgt tggtggatca ctcaattgtg gagagttttg gacaaggtgg gagaagagta    1740

ataacatcac gcatttatcc aacagaagca atatatggag cagcaaagct tttccttttc    1800

aacaatgcta catcagcaaa tgtgaaggca actgtgaaag tatggcaact caattctgct    1860

ttcattcaac cattttcctg a                                              1881


