﻿                         序列表

<110>  浙江大学

<120>  一种SETD4蛋白抑制剂在制备激活休眠肿瘤细胞药物中的应用

<160>  25

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  375
<212>  PRT
<213>  人源DEK蛋白(Humanized)

<400>  1
Met Ser Ala Ser Ala Pro Ala Ala Glu Gly Glu Gly Thr Pro Thr Gln 
1               5                   10                  15      
Pro Ala Ser Glu Lys Glu Pro Glu Met Pro Gly Pro Arg Glu Glu Ser 
            20                  25                  30          
Glu Glu Glu Glu Asp Glu Asp Asp Glu Glu Glu Glu Glu Glu Glu Lys 
        35                  40                  45              
Glu Lys Ser Leu Ile Val Glu Gly Lys Arg Glu Lys Lys Lys Val Glu 
    50                  55                  60                  
Arg Leu Thr Met Gln Val Ser Ser Leu Gln Arg Glu Pro Phe Thr Ile 
65                  70                  75                  80  
Ala Gln Gly Lys Gly Gln Lys Leu Cys Glu Ile Glu Arg Ile His Phe 
                85                  90                  95      
Phe Leu Ser Lys Lys Lys Thr Asp Glu Leu Arg Asn Leu His Lys Leu 
            100                 105                 110         
Leu Tyr Asn Arg Pro Gly Thr Val Ser Ser Leu Lys Lys Asn Val Gly 
        115                 120                 125             
Gln Phe Ser Gly Phe Pro Phe Glu Lys Gly Ser Val Gln Tyr Lys Lys 
    130                 135                 140                 
Lys Glu Glu Met Leu Lys Lys Phe Arg Asn Ala Met Leu Lys Ser Ile 
145                 150                 155                 160 
Cys Glu Val Leu Asp Leu Glu Arg Ser Gly Val Asn Ser Glu Leu Val 
                165                 170                 175     
Lys Arg Ile Leu Asn Phe Leu Met His Pro Lys Pro Ser Gly Lys Pro 
            180                 185                 190         
Leu Pro Lys Ser Lys Lys Thr Cys Ser Lys Gly Ser Lys Lys Glu Arg 
        195                 200                 205             
Asn Ser Ser Gly Met Ala Arg Lys Ala Lys Arg Thr Lys Cys Pro Glu 
    210                 215                 220                 
Ile Leu Ser Asp Glu Ser Ser Ser Asp Glu Asp Glu Lys Lys Asn Lys 
225                 230                 235                 240 
Glu Glu Ser Ser Asp Asp Glu Asp Lys Glu Ser Glu Glu Glu Pro Pro 
                245                 250                 255     
Lys Lys Thr Ala Lys Arg Glu Lys Pro Lys Gln Lys Ala Thr Ser Lys 
            260                 265                 270         
Ser Lys Lys Ser Val Lys Ser Ala Asn Val Lys Lys Ala Asp Ser Ser 
        275                 280                 285             
Thr Thr Lys Lys Asn Gln Asn Ser Ser Lys Lys Glu Ser Glu Ser Glu 
    290                 295                 300                 
Asp Ser Ser Asp Asp Glu Pro Leu Ile Lys Lys Leu Lys Lys Pro Pro 
305                 310                 315                 320 
Thr Asp Glu Glu Leu Lys Glu Thr Ile Lys Lys Leu Leu Ala Ser Ala 
                325                 330                 335     
Asn Leu Glu Glu Val Thr Met Lys Gln Ile Cys Lys Lys Val Tyr Glu 
            340                 345                 350         
Asn Tyr Pro Thr Tyr Asp Leu Thr Glu Arg Lys Asp Phe Ile Lys Thr 
        355                 360                 365             
Thr Val Lys Glu Leu Ile Ser 
    370                 375 

<210>  2
<211>  17
<212>  PRT
<213>  NLS结构域(Humanized)

<400>  2
Lys Lys Glu Arg Asn Ser Ser Gly Met Ala Arg Lys Ala Lys Arg Thr 
1               5                   10                  15      
Lys 
    

<210>  3
<211>  35
<212>  PRT
<213>  SAP结构域(Humanized)

<400>  3
Leu Lys Lys Phe Arg Asn Ala Met Leu Lys Ser Ile Cys Glu Val Leu 
1               5                   10                  15      
Asp Leu Glu Arg Ser Gly Val Asn Ser Glu Leu Val Lys Arg Ile Leu 
            20                  25                  30          
Asn Phe Leu 
        35  

<210>  4
<211>  39
<212>  PRT
<213>  pseudo-SAP结构域(Humanized)

<400>  4
Ile Glu Arg Ile His Phe Phe Leu Ser Lys Lys Lys Thr Asp Glu Leu 
1               5                   10                  15      
Arg Asn Leu His Lys Leu Leu Tyr Asn Arg Pro Gly Thr Val Ser Ser 
            20                  25                  30          
Leu Lys Lys Asn Val Gly Gln 
        35                  

<210>  5
<211>  1128
<212>  DNA
<213>  DEK基因(Humanized)

<400>  5
atgtccgcct cggcccctgc tgcggagggg gagggaaccc ccacccagcc cgcgtccgag        60
aaagaacccg aaatgcccgg tcccagagag gagagcgagg aggaagagga cgaggacgac       120
gaggaggagg aggaggagga aaaagaaaag agtctcatcg tggaaggcaa gagggaaaag       180
aaaaaagtag agaggttgac aatgcaagtc tcttccttac agagagagcc atttacaatt       240
gcacaaggaa aggggcagaa actttgtgaa attgagagga tacatttttt tctaagtaag       300
aagaaaaccg atgaacttag aaatctacac aaactgcttt acaacaggcc aggcactgtg       360
tcctcattaa agaagaatgt gggtcagttc agtggctttc catttgaaaa aggaagtgtc       420
caatataaaa agaaggaaga aatgttgaaa aaatttagaa atgccatgtt aaagagcatc       480
tgtgaggttc ttgatttgga gagatcaggt gtaaatagtg aactagtgaa gaggatcttg       540
aatttcttaa tgcatccaaa gccttctggc aaaccattgc cgaaatctaa aaaaacttgt       600
agcaaaggca gtaaaaagga acggaacagt tctggaatgg caaggaaggc taagcgaacc       660
aaatgtcctg aaattctgtc agatgaatct agtagtgatg aagatgaaaa gaaaaacaag       720
gaagagtctt cagatgatga agataaagaa agtgaagagg agccaccaaa aaagacagcc       780
aaaagagaaa aacctaaaca gaaagctact tctaaaagta aaaaatctgt gaaaagtgcc       840
aatgttaaga aagcagatag cagcaccacc aagaagaatc aaaacagttc caaaaaagaa       900
agtgagtctg aggatagttc agatgatgaa cctttaatta aaaagttgaa gaaaccccct       960
acagatgaag agttaaagga aacaataaag aaattactgg ccagtgctaa cttggaagaa      1020
gtcacaatga aacagatttg caaaaaggtc tatgaaaatt atcctactta tgatttaact      1080
gaaagaaaag atttcataaa aacaactgta aaagagctaa tttcttga                   1128

<210>  6
<211>  1881
<212>  DNA
<213>  EGFP-DEK基因(Humanized)

<400>  6
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac        60
ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac       120
ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc       180
ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag       240
cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc       300
ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg       360
gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac       420
aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac       480
ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc       540
gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac       600
tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc       660
ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc       720
ggactcagat ctcgagctca agcttcgaat tctatgtccg cctcggcccc tgctgcggag       780
ggggagggaa cccccaccca gcccgcgtcc gagaaagaac ccgaaatgcc cggtcccaga       840
gaggagagcg aggaggaaga ggacgaggac gacgaggagg aggaggagga ggaaaaagaa       900
aagagtctca tcgtggaagg caagagggaa aagaaaaaag tagagaggtt gacaatgcaa       960
gtctcttcct tacagagaga gccatttaca attgcacaag gaaaggggca gaaactttgt      1020
gaaattgaga ggatacattt ttttctaagt aagaagaaaa ccgatgaact tagaaatcta      1080
cacaaactgc tttacaacag gccaggcact gtgtcctcat taaagaagaa tgtgggtcag      1140
ttcagtggct ttccatttga aaaaggaagt gtccaatata aaaagaagga agaaatgttg      1200
aaaaaattta gaaatgccat gttaaagagc atctgtgagg ttcttgattt ggagagatca      1260
ggtgtaaata gtgaactagt gaagaggatc ttgaatttct taatgcatcc aaagccttct      1320
ggcaaaccat tgccgaaatc taaaaaaact tgtagcaaag gcagtaaaaa ggaacggaac      1380
agttctggaa tggcaaggaa ggctaagcga accaaatgtc ctgaaattct gtcagatgaa      1440
tctagtagtg atgaagatga aaagaaaaac aaggaagagt cttcagatga tgaagataaa      1500
gaaagtgaag aggagccacc aaaaaagaca gccaaaagag aaaaacctaa acagaaagct      1560
acttctaaaa gtaaaaaatc tgtgaaaagt gccaatgtta agaaagcaga tagcagcacc      1620
accaagaaga atcaaaacag ttccaaaaaa gaaagtgagt ctgaggatag ttcagatgat      1680
gaacctttaa ttaaaaagtt gaagaaaccc cctacagatg aagagttaaa ggaaacaata      1740
aagaaattac tggccagtgc taacttggaa gaagtcacaa tgaaacagat ttgcaaaaag      1800
gtctatgaaa attatcctac ttatgattta actgaaagaa aagatttcat aaaaacaact      1860
gtaaaagagc taatttcttg a                                                1881

<210>  7
<211>  626
<212>  PRT
<213>  EGFP-DEK(Humanized)

<400>  7
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
225                 230                 235                 240 
Gly Leu Arg Ser Arg Ala Gln Ala Ser Asn Ser Met Ser Ala Ser Ala 
                245                 250                 255     
Pro Ala Ala Glu Gly Glu Gly Thr Pro Thr Gln Pro Ala Ser Glu Lys 
            260                 265                 270         
Glu Pro Glu Met Pro Gly Pro Arg Glu Glu Ser Glu Glu Glu Glu Asp 
        275                 280                 285             
Glu Asp Asp Glu Glu Glu Glu Glu Glu Glu Lys Glu Lys Ser Leu Ile 
    290                 295                 300                 
Val Glu Gly Lys Arg Glu Lys Lys Lys Val Glu Arg Leu Thr Met Gln 
305                 310                 315                 320 
Val Ser Ser Leu Gln Arg Glu Pro Phe Thr Ile Ala Gln Gly Lys Gly 
                325                 330                 335     
Gln Lys Leu Cys Glu Ile Glu Arg Ile His Phe Phe Leu Ser Lys Lys 
            340                 345                 350         
Lys Thr Asp Glu Leu Arg Asn Leu His Lys Leu Leu Tyr Asn Arg Pro 
        355                 360                 365             
Gly Thr Val Ser Ser Leu Lys Lys Asn Val Gly Gln Phe Ser Gly Phe 
    370                 375                 380                 
Pro Phe Glu Lys Gly Ser Val Gln Tyr Lys Lys Lys Glu Glu Met Leu 
385                 390                 395                 400 
Lys Lys Phe Arg Asn Ala Met Leu Lys Ser Ile Cys Glu Val Leu Asp 
                405                 410                 415     
Leu Glu Arg Ser Gly Val Asn Ser Glu Leu Val Lys Arg Ile Leu Asn 
            420                 425                 430         
Phe Leu Met His Pro Lys Pro Ser Gly Lys Pro Leu Pro Lys Ser Lys 
        435                 440                 445             
Lys Thr Cys Ser Lys Gly Ser Lys Lys Glu Arg Asn Ser Ser Gly Met 
    450                 455                 460                 
Ala Arg Lys Ala Lys Arg Thr Lys Cys Pro Glu Ile Leu Ser Asp Glu 
465                 470                 475                 480 
Ser Ser Ser Asp Glu Asp Glu Lys Lys Asn Lys Glu Glu Ser Ser Asp 
                485                 490                 495     
Asp Glu Asp Lys Glu Ser Glu Glu Glu Pro Pro Lys Lys Thr Ala Lys 
            500                 505                 510         
Arg Glu Lys Pro Lys Gln Lys Ala Thr Ser Lys Ser Lys Lys Ser Val 
        515                 520                 525             
Lys Ser Ala Asn Val Lys Lys Ala Asp Ser Ser Thr Thr Lys Lys Asn 
    530                 535                 540                 
Gln Asn Ser Ser Lys Lys Glu Ser Glu Ser Glu Asp Ser Ser Asp Asp 
545                 550                 555                 560 
Glu Pro Leu Ile Lys Lys Leu Lys Lys Pro Pro Thr Asp Glu Glu Leu 
                565                 570                 575     
Lys Glu Thr Ile Lys Lys Leu Leu Ala Ser Ala Asn Leu Glu Glu Val 
            580                 585                 590         
Thr Met Lys Gln Ile Cys Lys Lys Val Tyr Glu Asn Tyr Pro Thr Tyr 
        595                 600                 605             
Asp Leu Thr Glu Arg Lys Asp Phe Ile Lys Thr Thr Val Lys Glu Leu 
    610                 615                 620                 
Ile Ser 
625     

<210>  8
<211>  1830
<212>  DNA
<213>  EGFP-DEKΔNLS基因(Humanized)

<400>  8
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac        60
ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac       120
ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc       180
ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag       240
cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc       300
ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg       360
gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac       420
aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac       480
ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc       540
gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac       600
tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc       660
ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc       720
ggactcagat ctcgagctca agcttcgaat tctatgtccg cctcggcccc tgctgcggag       780
ggggagggaa cccccaccca gcccgcgtcc gagaaagaac ccgaaatgcc cggtcccaga       840
gaggagagcg aggaggaaga ggacgaggac gacgaggagg aggaggagga ggaaaaagaa       900
aagagtctca tcgtggaagg caagagggaa aagaaaaaag tagagaggtt gacaatgcaa       960
gtctcttcct tacagagaga gccatttaca attgcacaag gaaaggggca gaaactttgt      1020
gaaattgaga ggatacattt ttttctaagt aagaagaaaa ccgatgaact tagaaatcta      1080
cacaaactgc tttacaacag gccaggcact gtgtcctcat taaagaagaa tgtgggtcag      1140
ttcagtggct ttccatttga aaaaggaagt gtccaatata aaaagaagga agaaatgttg      1200
aaaaaattta gaaatgccat gttaaagagc atctgtgagg ttcttgattt ggagagatca      1260
ggtgtaaata gtgaactagt gaagaggatc ttgaatttct taatgcatcc aaagccttct      1320
ggcaaaccat tgccgaaatc taaaaaaact tgtagcaaag gcagttgtcc tgaaattctg      1380
tcagatgaat ctagtagtga tgaagatgaa aagaaaaaca aggaagagtc ttcagatgat      1440
gaagataaag aaagtgaaga ggagccacca aaaaagacag ccaaaagaga aaaacctaaa      1500
cagaaagcta cttctaaaag taaaaaatct gtgaaaagtg ccaatgttaa gaaagcagat      1560
agcagcacca ccaagaagaa tcaaaacagt tccaaaaaag aaagtgagtc tgaggatagt      1620
tcagatgatg aacctttaat taaaaagttg aagaaacccc ctacagatga agagttaaag      1680
gaaacaataa agaaattact ggccagtgct aacttggaag aagtcacaat gaaacagatt      1740
tgcaaaaagg tctatgaaaa ttatcctact tatgatttaa ctgaaagaaa agatttcata      1800
aaaacaactg taaaagagct aatttcttga                                       1830

<210>  9
<211>  609
<212>  PRT
<213>  EGFP-DEKΔNLS基因(Humanized)

<400>  9
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
225                 230                 235                 240 
Gly Leu Arg Ser Arg Ala Gln Ala Ser Asn Ser Met Ser Ala Ser Ala 
                245                 250                 255     
Pro Ala Ala Glu Gly Glu Gly Thr Pro Thr Gln Pro Ala Ser Glu Lys 
            260                 265                 270         
Glu Pro Glu Met Pro Gly Pro Arg Glu Glu Ser Glu Glu Glu Glu Asp 
        275                 280                 285             
Glu Asp Asp Glu Glu Glu Glu Glu Glu Glu Lys Glu Lys Ser Leu Ile 
    290                 295                 300                 
Val Glu Gly Lys Arg Glu Lys Lys Lys Val Glu Arg Leu Thr Met Gln 
305                 310                 315                 320 
Val Ser Ser Leu Gln Arg Glu Pro Phe Thr Ile Ala Gln Gly Lys Gly 
                325                 330                 335     
Gln Lys Leu Cys Glu Ile Glu Arg Ile His Phe Phe Leu Ser Lys Lys 
            340                 345                 350         
Lys Thr Asp Glu Leu Arg Asn Leu His Lys Leu Leu Tyr Asn Arg Pro 
        355                 360                 365             
Gly Thr Val Ser Ser Leu Lys Lys Asn Val Gly Gln Phe Ser Gly Phe 
    370                 375                 380                 
Pro Phe Glu Lys Gly Ser Val Gln Tyr Lys Lys Lys Glu Glu Met Leu 
385                 390                 395                 400 
Lys Lys Phe Arg Asn Ala Met Leu Lys Ser Ile Cys Glu Val Leu Asp 
                405                 410                 415     
Leu Glu Arg Ser Gly Val Asn Ser Glu Leu Val Lys Arg Ile Leu Asn 
            420                 425                 430         
Phe Leu Met His Pro Lys Pro Ser Gly Lys Pro Leu Pro Lys Ser Lys 
        435                 440                 445             
Lys Thr Cys Ser Lys Gly Ser Cys Pro Glu Ile Leu Ser Asp Glu Ser 
    450                 455                 460                 
Ser Ser Asp Glu Asp Glu Lys Lys Asn Lys Glu Glu Ser Ser Asp Asp 
465                 470                 475                 480 
Glu Asp Lys Glu Ser Glu Glu Glu Pro Pro Lys Lys Thr Ala Lys Arg 
                485                 490                 495     
Glu Lys Pro Lys Gln Lys Ala Thr Ser Lys Ser Lys Lys Ser Val Lys 
            500                 505                 510         
Ser Ala Asn Val Lys Lys Ala Asp Ser Ser Thr Thr Lys Lys Asn Gln 
        515                 520                 525             
Asn Ser Ser Lys Lys Glu Ser Glu Ser Glu Asp Ser Ser Asp Asp Glu 
    530                 535                 540                 
Pro Leu Ile Lys Lys Leu Lys Lys Pro Pro Thr Asp Glu Glu Leu Lys 
545                 550                 555                 560 
Glu Thr Ile Lys Lys Leu Leu Ala Ser Ala Asn Leu Glu Glu Val Thr 
                565                 570                 575     
Met Lys Gln Ile Cys Lys Lys Val Tyr Glu Asn Tyr Pro Thr Tyr Asp 
            580                 585                 590         
Leu Thr Glu Arg Lys Asp Phe Ile Lys Thr Thr Val Lys Glu Leu Ile 
        595                 600                 605             
Ser 
    

<210>  10
<211>  1896
<212>  DNA
<213>  EGFP-DEK基因(Murine)

<400>  10
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac        60
ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac       120
ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc       180
ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag       240
cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc       300
ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg       360
gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac       420
aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac       480
ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc       540
gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac       600
tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc       660
ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc       720
ggactcagat ctcgagctca agcttcgaat tctatgtcgg cggcggcggc ccccgctgcg       780
gagggagagg acgcccccgt gccgccctca tccgagaagg aacccgagat gccgggtccc       840
agggaagaga gtgaggagga ggaggaggat gacgaagacg atgatgaaga ggacgaggag       900
gaagaaaaag aaaagagtct tatcgtggaa ggcaagagag agaagaagaa agtagagaga       960
ctgacgatgc aagtgtcttc cttacagaga gagccattta cagtgacaca agggaagggt      1020
cagaaacttt gtgaaattga aaggatacat ttctttctga gtaagaaaaa accagatgaa      1080
cttagaaatc tacacaaact gctttacaac aggccgggca cagtgtcctc gttgaagaag      1140
aacgtgggtc agttcagtgg ctttccattc gaaaaaggca gtacccagta taaaaagaag      1200
gaagaaatgt tgaaaaagtt tcgaaatgcc atgttaaaga gcatctgtga ggttcttgat      1260
ttagagaggt caggcgtgaa cagcgaactc gtgaagagga tcttgaactt cttaatgcat      1320
ccaaagcctt ctggcaaacc attaccaaaa tccaaaaaat cttccagcaa aggtagtaaa      1380
aaggaacgga acagttctgg aacaacaagg aagtcaaagc aaactaaatg ccctgaaatt      1440
ctgtcagatg agtctagtag tgatgaagat gagaagaaaa ataaggaaga gtcttcggaa      1500
gatgaagaga aagaaagtga agaggagcaa ccaccaaaaa agacatctaa aaaagaaaaa      1560
gcaaaacaga aagctactgc taaaagtaaa aaatctgtga agagtgctaa tgttaagaag      1620
gcagacagca gtaccaccaa gaagaatcaa aaaagttcca aaaaagagtc tgaatctgaa      1680
gacagttctg atgatgaacc cttaattaaa aaattgaaaa agccacctac agatgaagag      1740
ctaaaggaaa cagtgaagaa attactggct gatgctaact tggaagaagt cacaatgaag      1800
cagatttgca aagaggtata tgaaaattat cctgcttatg atttgactga gaggaaagat      1860
ttcattaaaa caactgtaaa agagctaatt tcttga                                1896

<210>  11
<211>  631
<212>  PRT
<213>  EGFP-DEK基因(Murine)

<400>  11
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
225                 230                 235                 240 
Gly Leu Arg Ser Arg Ala Gln Ala Ser Asn Ser Met Ser Ala Ala Ala 
                245                 250                 255     
Ala Pro Ala Ala Glu Gly Glu Asp Ala Pro Val Pro Pro Ser Ser Glu 
            260                 265                 270         
Lys Glu Pro Glu Met Pro Gly Pro Arg Glu Glu Ser Glu Glu Glu Glu 
        275                 280                 285             
Glu Asp Asp Glu Asp Asp Asp Glu Glu Asp Glu Glu Glu Glu Lys Glu 
    290                 295                 300                 
Lys Ser Leu Ile Val Glu Gly Lys Arg Glu Lys Lys Lys Val Glu Arg 
305                 310                 315                 320 
Leu Thr Met Gln Val Ser Ser Leu Gln Arg Glu Pro Phe Thr Val Thr 
                325                 330                 335     
Gln Gly Lys Gly Gln Lys Leu Cys Glu Ile Glu Arg Ile His Phe Phe 
            340                 345                 350         
Leu Ser Lys Lys Lys Pro Asp Glu Leu Arg Asn Leu His Lys Leu Leu 
        355                 360                 365             
Tyr Asn Arg Pro Gly Thr Val Ser Ser Leu Lys Lys Asn Val Gly Gln 
    370                 375                 380                 
Phe Ser Gly Phe Pro Phe Glu Lys Gly Ser Thr Gln Tyr Lys Lys Lys 
385                 390                 395                 400 
Glu Glu Met Leu Lys Lys Phe Arg Asn Ala Met Leu Lys Ser Ile Cys 
                405                 410                 415     
Glu Val Leu Asp Leu Glu Arg Ser Gly Val Asn Ser Glu Leu Val Lys 
            420                 425                 430         
Arg Ile Leu Asn Phe Leu Met His Pro Lys Pro Ser Gly Lys Pro Leu 
        435                 440                 445             
Pro Lys Ser Lys Lys Ser Ser Ser Lys Gly Ser Lys Lys Glu Arg Asn 
    450                 455                 460                 
Ser Ser Gly Thr Thr Arg Lys Ser Lys Gln Thr Lys Cys Pro Glu Ile 
465                 470                 475                 480 
Leu Ser Asp Glu Ser Ser Ser Asp Glu Asp Glu Lys Lys Asn Lys Glu 
                485                 490                 495     
Glu Ser Ser Glu Asp Glu Glu Lys Glu Ser Glu Glu Glu Gln Pro Pro 
            500                 505                 510         
Lys Lys Thr Ser Lys Lys Glu Lys Ala Lys Gln Lys Ala Thr Ala Lys 
        515                 520                 525             
Ser Lys Lys Ser Val Lys Ser Ala Asn Val Lys Lys Ala Asp Ser Ser 
    530                 535                 540                 
Thr Thr Lys Lys Asn Gln Lys Ser Ser Lys Lys Glu Ser Glu Ser Glu 
545                 550                 555                 560 
Asp Ser Ser Asp Asp Glu Pro Leu Ile Lys Lys Leu Lys Lys Pro Pro 
                565                 570                 575     
Thr Asp Glu Glu Leu Lys Glu Thr Val Lys Lys Leu Leu Ala Asp Ala 
            580                 585                 590         
Asn Leu Glu Glu Val Thr Met Lys Gln Ile Cys Lys Glu Val Tyr Glu 
        595                 600                 605             
Asn Tyr Pro Ala Tyr Asp Leu Thr Glu Arg Lys Asp Phe Ile Lys Thr 
    610                 615                 620                 
Thr Val Lys Glu Leu Ile Ser 
625                 630     

<210>  12
<211>  1845
<212>  DNA
<213>  EGFP-DEKΔNLS基因(Murine)

<400>  12
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac        60
ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac       120
ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc       180
ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag       240
cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc       300
ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg       360
gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac       420
aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac       480
ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc       540
gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac       600
tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc       660
ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc       720
ggactcagat ctcgagctca agcttcgaat tctatgtcgg cggcggcggc ccccgctgcg       780
gagggagagg acgcccccgt gccgccctca tccgagaagg aacccgagat gccgggtccc       840
agggaagaga gtgaggagga ggaggaggat gacgaagacg atgatgaaga ggacgaggag       900
gaagaaaaag aaaagagtct tatcgtggaa ggcaagagag agaagaagaa agtagagaga       960
ctgacgatgc aagtgtcttc cttacagaga gagccattta cagtgacaca agggaagggt      1020
cagaaacttt gtgaaattga aaggatacat ttctttctga gtaagaaaaa accagatgaa      1080
cttagaaatc tacacaaact gctttacaac aggccgggca cagtgtcctc gttgaagaag      1140
aacgtgggtc agttcagtgg ctttccattc gaaaaaggca gtacccagta taaaaagaag      1200
gaagaaatgt tgaaaaagtt tcgaaatgcc atgttaaaga gcatctgtga ggttcttgat      1260
ttagagaggt caggcgtgaa cagcgaactc gtgaagagga tcttgaactt cttaatgcat      1320
ccaaagcctt ctggcaaacc attaccaaaa tccaaaaaat cttccagcaa aggtagttgc      1380
cctgaaattc tgtcagatga gtctagtagt gatgaagatg agaagaaaaa taaggaagag      1440
tcttcggaag atgaagagaa agaaagtgaa gaggagcaac caccaaaaaa gacatctaaa      1500
aaagaaaaag caaaacagaa agctactgct aaaagtaaaa aatctgtgaa gagtgctaat      1560
gttaagaagg cagacagcag taccaccaag aagaatcaaa aaagttccaa aaaagagtct      1620
gaatctgaag acagttctga tgatgaaccc ttaattaaaa aattgaaaaa gccacctaca      1680
gatgaagagc taaaggaaac agtgaagaaa ttactggctg atgctaactt ggaagaagtc      1740
acaatgaagc agatttgcaa agaggtatat gaaaattatc ctgcttatga tttgactgag      1800
aggaaagatt tcattaaaac aactgtaaaa gagctaattt cttga                      1845

<210>  13
<211>  614
<212>  PRT
<213>  EGFP-DEKΔNLS(Murine)

<400>  13
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
225                 230                 235                 240 
Gly Leu Arg Ser Arg Ala Gln Ala Ser Asn Ser Met Ser Ala Ala Ala 
                245                 250                 255     
Ala Pro Ala Ala Glu Gly Glu Asp Ala Pro Val Pro Pro Ser Ser Glu 
            260                 265                 270         
Lys Glu Pro Glu Met Pro Gly Pro Arg Glu Glu Ser Glu Glu Glu Glu 
        275                 280                 285             
Glu Asp Asp Glu Asp Asp Asp Glu Glu Asp Glu Glu Glu Glu Lys Glu 
    290                 295                 300                 
Lys Ser Leu Ile Val Glu Gly Lys Arg Glu Lys Lys Lys Val Glu Arg 
305                 310                 315                 320 
Leu Thr Met Gln Val Ser Ser Leu Gln Arg Glu Pro Phe Thr Val Thr 
                325                 330                 335     
Gln Gly Lys Gly Gln Lys Leu Cys Glu Ile Glu Arg Ile His Phe Phe 
            340                 345                 350         
Leu Ser Lys Lys Lys Pro Asp Glu Leu Arg Asn Leu His Lys Leu Leu 
        355                 360                 365             
Tyr Asn Arg Pro Gly Thr Val Ser Ser Leu Lys Lys Asn Val Gly Gln 
    370                 375                 380                 
Phe Ser Gly Phe Pro Phe Glu Lys Gly Ser Thr Gln Tyr Lys Lys Lys 
385                 390                 395                 400 
Glu Glu Met Leu Lys Lys Phe Arg Asn Ala Met Leu Lys Ser Ile Cys 
                405                 410                 415     
Glu Val Leu Asp Leu Glu Arg Ser Gly Val Asn Ser Glu Leu Val Lys 
            420                 425                 430         
Arg Ile Leu Asn Phe Leu Met His Pro Lys Pro Ser Gly Lys Pro Leu 
        435                 440                 445             
Pro Lys Ser Lys Lys Ser Ser Ser Lys Gly Ser Cys Pro Glu Ile Leu 
    450                 455                 460                 
Ser Asp Glu Ser Ser Ser Asp Glu Asp Glu Lys Lys Asn Lys Glu Glu 
465                 470                 475                 480 
Ser Ser Glu Asp Glu Glu Lys Glu Ser Glu Glu Glu Gln Pro Pro Lys 
                485                 490                 495     
Lys Thr Ser Lys Lys Glu Lys Ala Lys Gln Lys Ala Thr Ala Lys Ser 
            500                 505                 510         
Lys Lys Ser Val Lys Ser Ala Asn Val Lys Lys Ala Asp Ser Ser Thr 
        515                 520                 525             
Thr Lys Lys Asn Gln Lys Ser Ser Lys Lys Glu Ser Glu Ser Glu Asp 
    530                 535                 540                 
Ser Ser Asp Asp Glu Pro Leu Ile Lys Lys Leu Lys Lys Pro Pro Thr 
545                 550                 555                 560 
Asp Glu Glu Leu Lys Glu Thr Val Lys Lys Leu Leu Ala Asp Ala Asn 
                565                 570                 575     
Leu Glu Glu Val Thr Met Lys Gln Ile Cys Lys Glu Val Tyr Glu Asn 
            580                 585                 590         
Tyr Pro Ala Tyr Asp Leu Thr Glu Arg Lys Asp Phe Ile Lys Thr Thr 
        595                 600                 605             
Val Lys Glu Leu Ile Ser 
    610                 

<210>  14
<211>  20
<212>  DNA
<213>  未知(Unknown)

<400>  14
ggatagttca gatgatgaac                                                    20

<210>  15
<211>  20
<212>  DNA
<213>  未知(Unknown)

<400>  15
gtgatgaaga tgaaaagaaa                                                    20

<210>  16
<211>  21
<212>  DNA
<213>  未知(Unknown)

<400>  16
gtgaagaaat tactggctga t                                                  21

<210>  17
<211>  21
<212>  DNA
<213>  未知(Unknown)

<400>  17
cgaactcgtg aagaggatct t                                                  21

<210>  18
<211>  21
<212>  DNA
<213>  未知(Unknown)

<400>  18
atgttaacag ctgtactggt g                                                  21

<210>  19
<211>  1077
<212>  DNA
<213>  DEKΔNLS基因(Humanized)

<400>  19
atgtccgcct cggcccctgc tgcggagggg gagggaaccc ccacccagcc cgcgtccgag        60
aaagaacccg aaatgcccgg tcccagagag gagagcgagg aggaagagga cgaggacgac       120
gaggaggagg aggaggagga aaaagaaaag agtctcatcg tggaaggcaa gagggaaaag       180
aaaaaagtag agaggttgac aatgcaagtc tcttccttac agagagagcc atttacaatt       240
gcacaaggaa aggggcagaa actttgtgaa attgagagga tacatttttt tctaagtaag       300
aagaaaaccg atgaacttag aaatctacac aaactgcttt acaacaggcc aggcactgtg       360
tcctcattaa agaagaatgt gggtcagttc agtggctttc catttgaaaa aggaagtgtc       420
caatataaaa agaaggaaga aatgttgaaa aaatttagaa atgccatgtt aaagagcatc       480
tgtgaggttc ttgatttgga gagatcaggt gtaaatagtg aactagtgaa gaggatcttg       540
aatttcttaa tgcatccaaa gccttctggc aaaccattgc cgaaatctaa aaaaacttgt       600
agcaaaggca gttgtcctga aattctgtca gatgaatcta gtagtgatga agatgaaaag       660
aaaaacaagg aagagtcttc agatgatgaa gataaagaaa gtgaagagga gccaccaaaa       720
aagacagcca aaagagaaaa acctaaacag aaagctactt ctaaaagtaa aaaatctgtg       780
aaaagtgcca atgttaagaa agcagatagc agcaccacca agaagaatca aaacagttcc       840
aaaaaagaaa gtgagtctga ggatagttca gatgatgaac ctttaattaa aaagttgaag       900
aaacccccta cagatgaaga gttaaaggaa acaataaaga aattactggc cagtgctaac       960
ttggaagaag tcacaatgaa acagatttgc aaaaaggtct atgaaaatta tcctacttat      1020
gatttaactg aaagaaaaga tttcataaaa acaactgtaa aagagctaat ttcttga         1077

<210>  20
<211>  358
<212>  PRT
<213>  DEKΔNLS(Humanized)

<400>  20
Met Ser Ala Ser Ala Pro Ala Ala Glu Gly Glu Gly Thr Pro Thr Gln 
1               5                   10                  15      
Pro Ala Ser Glu Lys Glu Pro Glu Met Pro Gly Pro Arg Glu Glu Ser 
            20                  25                  30          
Glu Glu Glu Glu Asp Glu Asp Asp Glu Glu Glu Glu Glu Glu Glu Lys 
        35                  40                  45              
Glu Lys Ser Leu Ile Val Glu Gly Lys Arg Glu Lys Lys Lys Val Glu 
    50                  55                  60                  
Arg Leu Thr Met Gln Val Ser Ser Leu Gln Arg Glu Pro Phe Thr Ile 
65                  70                  75                  80  
Ala Gln Gly Lys Gly Gln Lys Leu Cys Glu Ile Glu Arg Ile His Phe 
                85                  90                  95      
Phe Leu Ser Lys Lys Lys Thr Asp Glu Leu Arg Asn Leu His Lys Leu 
            100                 105                 110         
Leu Tyr Asn Arg Pro Gly Thr Val Ser Ser Leu Lys Lys Asn Val Gly 
        115                 120                 125             
Gln Phe Ser Gly Phe Pro Phe Glu Lys Gly Ser Val Gln Tyr Lys Lys 
    130                 135                 140                 
Lys Glu Glu Met Leu Lys Lys Phe Arg Asn Ala Met Leu Lys Ser Ile 
145                 150                 155                 160 
Cys Glu Val Leu Asp Leu Glu Arg Ser Gly Val Asn Ser Glu Leu Val 
                165                 170                 175     
Lys Arg Ile Leu Asn Phe Leu Met His Pro Lys Pro Ser Gly Lys Pro 
            180                 185                 190         
Leu Pro Lys Ser Lys Lys Thr Cys Ser Lys Gly Ser Cys Pro Glu Ile 
        195                 200                 205             
Leu Ser Asp Glu Ser Ser Ser Asp Glu Asp Glu Lys Lys Asn Lys Glu 
    210                 215                 220                 
Glu Ser Ser Asp Asp Glu Asp Lys Glu Ser Glu Glu Glu Pro Pro Lys 
225                 230                 235                 240 
Lys Thr Ala Lys Arg Glu Lys Pro Lys Gln Lys Ala Thr Ser Lys Ser 
                245                 250                 255     
Lys Lys Ser Val Lys Ser Ala Asn Val Lys Lys Ala Asp Ser Ser Thr 
            260                 265                 270         
Thr Lys Lys Asn Gln Asn Ser Ser Lys Lys Glu Ser Glu Ser Glu Asp 
        275                 280                 285             
Ser Ser Asp Asp Glu Pro Leu Ile Lys Lys Leu Lys Lys Pro Pro Thr 
    290                 295                 300                 
Asp Glu Glu Leu Lys Glu Thr Ile Lys Lys Leu Leu Ala Ser Ala Asn 
305                 310                 315                 320 
Leu Glu Glu Val Thr Met Lys Gln Ile Cys Lys Lys Val Tyr Glu Asn 
                325                 330                 335     
Tyr Pro Thr Tyr Asp Leu Thr Glu Arg Lys Asp Phe Ile Lys Thr Thr 
            340                 345                 350         
Val Lys Glu Leu Ile Ser 
        355             

<210>  21
<211>  1143
<212>  DNA
<213>  DEK基因(Murine)

<400>  21
atgtcggcgg cggcggcccc cgctgcggag ggagaggacg cccccgtgcc gccctcatcc        60
gagaaggaac ccgagatgcc gggtcccagg gaagagagtg aggaggagga ggaggatgac       120
gaagacgatg atgaagagga cgaggaggaa gaaaaagaaa agagtcttat cgtggaaggc       180
aagagagaga agaagaaagt agagagactg acgatgcaag tgtcttcctt acagagagag       240
ccatttacag tgacacaagg gaagggtcag aaactttgtg aaattgaaag gatacatttc       300
tttctgagta agaaaaaacc agatgaactt agaaatctac acaaactgct ttacaacagg       360
ccgggcacag tgtcctcgtt gaagaagaac gtgggtcagt tcagtggctt tccattcgaa       420
aaaggcagta cccagtataa aaagaaggaa gaaatgttga aaaagtttcg aaatgccatg       480
ttaaagagca tctgtgaggt tcttgattta gagaggtcag gcgtgaacag cgaactcgtg       540
aagaggatct tgaacttctt aatgcatcca aagccttctg gcaaaccatt accaaaatcc       600
aaaaaatctt ccagcaaagg tagtaaaaag gaacggaaca gttctggaac aacaaggaag       660
tcaaagcaaa ctaaatgccc tgaaattctg tcagatgagt ctagtagtga tgaagatgag       720
aagaaaaata aggaagagtc ttcggaagat gaagagaaag aaagtgaaga ggagcaacca       780
ccaaaaaaga catctaaaaa agaaaaagca aaacagaaag ctactgctaa aagtaaaaaa       840
tctgtgaaga gtgctaatgt taagaaggca gacagcagta ccaccaagaa gaatcaaaaa       900
agttccaaaa aagagtctga atctgaagac agttctgatg atgaaccctt aattaaaaaa       960
ttgaaaaagc cacctacaga tgaagagcta aaggaaacag tgaagaaatt actggctgat      1020
gctaacttgg aagaagtcac aatgaagcag atttgcaaag aggtatatga aaattatcct      1080
gcttatgatt tgactgagag gaaagatttc attaaaacaa ctgtaaaaga gctaatttct      1140
tga                                                                    1143

<210>  22
<211>  380
<212>  PRT
<213>  DEK(Murine)

<400>  22
Met Ser Ala Ala Ala Ala Pro Ala Ala Glu Gly Glu Asp Ala Pro Val 
1               5                   10                  15      
Pro Pro Ser Ser Glu Lys Glu Pro Glu Met Pro Gly Pro Arg Glu Glu 
            20                  25                  30          
Ser Glu Glu Glu Glu Glu Asp Asp Glu Asp Asp Asp Glu Glu Asp Glu 
        35                  40                  45              
Glu Glu Glu Lys Glu Lys Ser Leu Ile Val Glu Gly Lys Arg Glu Lys 
    50                  55                  60                  
Lys Lys Val Glu Arg Leu Thr Met Gln Val Ser Ser Leu Gln Arg Glu 
65                  70                  75                  80  
Pro Phe Thr Val Thr Gln Gly Lys Gly Gln Lys Leu Cys Glu Ile Glu 
                85                  90                  95      
Arg Ile His Phe Phe Leu Ser Lys Lys Lys Pro Asp Glu Leu Arg Asn 
            100                 105                 110         
Leu His Lys Leu Leu Tyr Asn Arg Pro Gly Thr Val Ser Ser Leu Lys 
        115                 120                 125             
Lys Asn Val Gly Gln Phe Ser Gly Phe Pro Phe Glu Lys Gly Ser Thr 
    130                 135                 140                 
Gln Tyr Lys Lys Lys Glu Glu Met Leu Lys Lys Phe Arg Asn Ala Met 
145                 150                 155                 160 
Leu Lys Ser Ile Cys Glu Val Leu Asp Leu Glu Arg Ser Gly Val Asn 
                165                 170                 175     
Ser Glu Leu Val Lys Arg Ile Leu Asn Phe Leu Met His Pro Lys Pro 
            180                 185                 190         
Ser Gly Lys Pro Leu Pro Lys Ser Lys Lys Ser Ser Ser Lys Gly Ser 
        195                 200                 205             
Lys Lys Glu Arg Asn Ser Ser Gly Thr Thr Arg Lys Ser Lys Gln Thr 
    210                 215                 220                 
Lys Cys Pro Glu Ile Leu Ser Asp Glu Ser Ser Ser Asp Glu Asp Glu 
225                 230                 235                 240 
Lys Lys Asn Lys Glu Glu Ser Ser Glu Asp Glu Glu Lys Glu Ser Glu 
                245                 250                 255     
Glu Glu Gln Pro Pro Lys Lys Thr Ser Lys Lys Glu Lys Ala Lys Gln 
            260                 265                 270         
Lys Ala Thr Ala Lys Ser Lys Lys Ser Val Lys Ser Ala Asn Val Lys 
        275                 280                 285             
Lys Ala Asp Ser Ser Thr Thr Lys Lys Asn Gln Lys Ser Ser Lys Lys 
    290                 295                 300                 
Glu Ser Glu Ser Glu Asp Ser Ser Asp Asp Glu Pro Leu Ile Lys Lys 
305                 310                 315                 320 
Leu Lys Lys Pro Pro Thr Asp Glu Glu Leu Lys Glu Thr Val Lys Lys 
                325                 330                 335     
Leu Leu Ala Asp Ala Asn Leu Glu Glu Val Thr Met Lys Gln Ile Cys 
            340                 345                 350         
Lys Glu Val Tyr Glu Asn Tyr Pro Ala Tyr Asp Leu Thr Glu Arg Lys 
        355                 360                 365             
Asp Phe Ile Lys Thr Thr Val Lys Glu Leu Ile Ser 
    370                 375                 380 

<210>  23
<211>  1092
<212>  DNA
<213>  DEKΔNLS(Murine)

<400>  23
atgtcggcgg cggcggcccc cgctgcggag ggagaggacg cccccgtgcc gccctcatcc        60
gagaaggaac ccgagatgcc gggtcccagg gaagagagtg aggaggagga ggaggatgac       120
gaagacgatg atgaagagga cgaggaggaa gaaaaagaaa agagtcttat cgtggaaggc       180
aagagagaga agaagaaagt agagagactg acgatgcaag tgtcttcctt acagagagag       240
ccatttacag tgacacaagg gaagggtcag aaactttgtg aaattgaaag gatacatttc       300
tttctgagta agaaaaaacc agatgaactt agaaatctac acaaactgct ttacaacagg       360
ccgggcacag tgtcctcgtt gaagaagaac gtgggtcagt tcagtggctt tccattcgaa       420
aaaggcagta cccagtataa aaagaaggaa gaaatgttga aaaagtttcg aaatgccatg       480
ttaaagagca tctgtgaggt tcttgattta gagaggtcag gcgtgaacag cgaactcgtg       540
aagaggatct tgaacttctt aatgcatcca aagccttctg gcaaaccatt accaaaatcc       600
aaaaaatctt ccagcaaagg tagttgccct gaaattctgt cagatgagtc tagtagtgat       660
gaagatgaga agaaaaataa ggaagagtct tcggaagatg aagagaaaga aagtgaagag       720
gagcaaccac caaaaaagac atctaaaaaa gaaaaagcaa aacagaaagc tactgctaaa       780
agtaaaaaat ctgtgaagag tgctaatgtt aagaaggcag acagcagtac caccaagaag       840
aatcaaaaaa gttccaaaaa agagtctgaa tctgaagaca gttctgatga tgaaccctta       900
attaaaaaat tgaaaaagcc acctacagat gaagagctaa aggaaacagt gaagaaatta       960
ctggctgatg ctaacttgga agaagtcaca atgaagcaga tttgcaaaga ggtatatgaa      1020
aattatcctg cttatgattt gactgagagg aaagatttca ttaaaacaac tgtaaaagag      1080
ctaatttctt ga                                                          1092

<210>  24
<211>  363
<212>  PRT
<213>  DEKΔNLS(Murine)

<400>  24
Met Ser Ala Ala Ala Ala Pro Ala Ala Glu Gly Glu Asp Ala Pro Val 
1               5                   10                  15      
Pro Pro Ser Ser Glu Lys Glu Pro Glu Met Pro Gly Pro Arg Glu Glu 
            20                  25                  30          
Ser Glu Glu Glu Glu Glu Asp Asp Glu Asp Asp Asp Glu Glu Asp Glu 
        35                  40                  45              
Glu Glu Glu Lys Glu Lys Ser Leu Ile Val Glu Gly Lys Arg Glu Lys 
    50                  55                  60                  
Lys Lys Val Glu Arg Leu Thr Met Gln Val Ser Ser Leu Gln Arg Glu 
65                  70                  75                  80  
Pro Phe Thr Val Thr Gln Gly Lys Gly Gln Lys Leu Cys Glu Ile Glu 
                85                  90                  95      
Arg Ile His Phe Phe Leu Ser Lys Lys Lys Pro Asp Glu Leu Arg Asn 
            100                 105                 110         
Leu His Lys Leu Leu Tyr Asn Arg Pro Gly Thr Val Ser Ser Leu Lys 
        115                 120                 125             
Lys Asn Val Gly Gln Phe Ser Gly Phe Pro Phe Glu Lys Gly Ser Thr 
    130                 135                 140                 
Gln Tyr Lys Lys Lys Glu Glu Met Leu Lys Lys Phe Arg Asn Ala Met 
145                 150                 155                 160 
Leu Lys Ser Ile Cys Glu Val Leu Asp Leu Glu Arg Ser Gly Val Asn 
                165                 170                 175     
Ser Glu Leu Val Lys Arg Ile Leu Asn Phe Leu Met His Pro Lys Pro 
            180                 185                 190         
Ser Gly Lys Pro Leu Pro Lys Ser Lys Lys Ser Ser Ser Lys Gly Ser 
        195                 200                 205             
Cys Pro Glu Ile Leu Ser Asp Glu Ser Ser Ser Asp Glu Asp Glu Lys 
    210                 215                 220                 
Lys Asn Lys Glu Glu Ser Ser Glu Asp Glu Glu Lys Glu Ser Glu Glu 
225                 230                 235                 240 
Glu Gln Pro Pro Lys Lys Thr Ser Lys Lys Glu Lys Ala Lys Gln Lys 
                245                 250                 255     
Ala Thr Ala Lys Ser Lys Lys Ser Val Lys Ser Ala Asn Val Lys Lys 
            260                 265                 270         
Ala Asp Ser Ser Thr Thr Lys Lys Asn Gln Lys Ser Ser Lys Lys Glu 
        275                 280                 285             
Ser Glu Ser Glu Asp Ser Ser Asp Asp Glu Pro Leu Ile Lys Lys Leu 
    290                 295                 300                 
Lys Lys Pro Pro Thr Asp Glu Glu Leu Lys Glu Thr Val Lys Lys Leu 
305                 310                 315                 320 
Leu Ala Asp Ala Asn Leu Glu Glu Val Thr Met Lys Gln Ile Cys Lys 
                325                 330                 335     
Glu Val Tyr Glu Asn Tyr Pro Ala Tyr Asp Leu Thr Glu Arg Lys Asp 
            340                 345                 350         
Phe Ile Lys Thr Thr Val Lys Glu Leu Ile Ser 
        355                 360             

<210>  25
<211>  375
<212>  PRT
<213>  未知(Unknown)

<400>  25
Met Ser Ala Xaa Ala Pro Ala Ala Glu Gly Glu Xaa Xaa Pro Xaa Xaa 
1               5                   10                  15      
Pro Xaa Ser Glu Lys Glu Pro Glu Met Pro Gly Pro Arg Glu Glu Ser 
            20                  25                  30          
Glu Glu Glu Glu Xaa Xaa Asp Asp Glu Glu Xaa Glu Glu Glu Glu Lys 
        35                  40                  45              
Glu Lys Ser Leu Ile Val Glu Gly Lys Arg Glu Lys Lys Lys Val Glu 
    50                  55                  60                  
Arg Leu Thr Met Gln Val Ser Ser Leu Gln Arg Glu Pro Phe Thr Xaa 
65                  70                  75                  80  
Xaa Gln Gly Lys Gly Gln Lys Leu Cys Glu Ile Glu Arg Ile His Phe 
                85                  90                  95      
Phe Leu Ser Lys Lys Lys Xaa Asp Glu Leu Arg Asn Leu His Lys Leu 
            100                 105                 110         
Leu Tyr Asn Arg Pro Gly Thr Val Ser Ser Leu Lys Lys Asn Val Gly 
        115                 120                 125             
Gln Phe Ser Gly Phe Pro Phe Glu Lys Gly Ser Xaa Gln Tyr Lys Lys 
    130                 135                 140                 
Lys Glu Glu Met Leu Lys Lys Phe Arg Asn Ala Met Leu Lys Ser Ile 
145                 150                 155                 160 
Cys Glu Val Leu Asp Leu Glu Arg Ser Gly Val Asn Ser Glu Leu Val 
                165                 170                 175     
Lys Arg Ile Leu Asn Phe Leu Met His Pro Lys Pro Ser Gly Lys Pro 
            180                 185                 190         
Leu Pro Lys Ser Lys Lys Xaa Xaa Ser Lys Gly Ser Lys Lys Glu Arg 
        195                 200                 205             
Asn Ser Ser Gly Xaa Xaa Arg Lys Xaa Lys Xaa Thr Lys Cys Pro Glu 
    210                 215                 220                 
Ile Leu Ser Asp Glu Ser Ser Ser Asp Glu Asp Glu Lys Lys Asn Lys 
225                 230                 235                 240 
Glu Glu Ser Ser Xaa Asp Glu Xaa Lys Glu Ser Glu Glu Glu Pro Pro 
                245                 250                 255     
Lys Lys Thr Xaa Lys Xaa Glu Lys Xaa Lys Gln Lys Ala Thr Xaa Lys 
            260                 265                 270         
Ser Lys Lys Ser Val Lys Ser Ala Asn Val Lys Lys Ala Asp Ser Ser 
        275                 280                 285             
Thr Thr Lys Lys Asn Gln Xaa Ser Ser Lys Lys Glu Ser Glu Ser Glu 
    290                 295                 300                 
Asp Ser Ser Asp Asp Glu Pro Leu Ile Lys Lys Leu Lys Lys Pro Pro 
305                 310                 315                 320 
Thr Asp Glu Glu Leu Lys Glu Thr Xaa Lys Lys Leu Leu Ala Xaa Ala 
                325                 330                 335     
Asn Leu Glu Glu Val Thr Met Lys Gln Ile Cys Lys Xaa Val Tyr Glu 
            340                 345                 350         
Asn Tyr Pro Xaa Tyr Asp Leu Thr Glu Arg Lys Asp Phe Ile Lys Thr 
        355                 360                 365             
Thr Val Lys Glu Leu Ile Ser 
    370                 375 

