                         SEQUENCE LISTING

<110>  Index Biosystems Inc.
 
<120>  METHODS AND COMPOSITIONS OF MATTER FOR INERT BIOENGINEERING OF A 
       BIOLOGICAL ENTITY

<130>  08948485WO

<140>  PCT/CA2022/050865
<141>  2022-05-27

<150>  US 63/194,761
<151>  2021-05-28

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  313
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  complete sequence of an inert cassette

<400>  1
ttaattaatt atttatatat ttaaatttag gacactcccc ccacttcatt caggctgcgc       60

aactgaggct gagggtggac ctgatcaacc tgaaccagga ccccagttta attaattaac      120

ccccacgaga gcgagttccc cgacactcgg ccaggtgccc accgtggtga gcctggagca      180

cttccccaag ggccacgcct acaccgccct ggagtgcggg cctcttcgct attacgccag      240

ctggcgaaag ggggatctgc tgggctagtt aattgctcag cggttaatta tattatattt      300

atatattaat taa                                                         313


<210>  2
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  stop codons

<400>  2
ttaattaatt atttatatat ttaaa                                             25


<210>  3
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  qPCR primer forward (Top strand)

<400>  3
tttaggacac tccccccact tc                                                22


<210>  4
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LAMP F3

<400>  4
actcccccca cttcattca                                                    19


<210>  5
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LAMP FIP 2

<400>  5
gctgagggtg gacctgat                                                     18


<210>  6
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  optimal stop codon configuration

<400>  6
aattaattaa tt                                                           12


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FIP 1

<400>  7
cacgagagcg agttccccga                                                   20


<210>  8
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe qPCR

<400>  8
tcggccaggt gcccaccgtg gtg                                               23


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BIP 1

<400>  9
gtggtgagcc tggagcactt                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BIP 2

<400>  10
cctcttcgct attacgccag                                                   20


<210>  11
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LAMP B3

<400>  11
cgaaaggggg atctgctg                                                     18


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  qPCR primer reverse (5' - 3')

<400>  12
ccgctgagca attaactagc c                                                 21


<210>  13
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  stop codons

<400>  13
ttaattatat tatatttata tattaattaa                                        30


<210>  14
<211>  322
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  a complete sequence of an inert cassette

<400>  14
ttaattaatt aaaagcttta gtggcccccc taatcaagtt ccgctatcca gagtcgtcgt       60

gagcacgact gtctctccca gcgaatcgct acgagcaata gaccgcggac tcgcgaacga      120

acaatactaa gggggcctta gcgcgttaaa ggttaattaa ttaactggaa gcttcgccag      180

ttattccccc tatcgaaaga gtcgcagcca caattgccta tttcgtgaaa gggatcagag      240

ccctgatcgg aatcgatacc tcgcctgtac gggtcaatac ggaaaattcc aattgttgcg      300

acaaggccgg ttaattaatt aa                                               322


<210>  15
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  stop codons

<400>  15
ttaattaatt aa                                                           12


<210>  16
<211>  4
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM site

<400>  16
ttta                                                                     4


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA binding site

<400>  17
gtggcccccc taatcaagtt c                                                 21


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequencing primer forward

<400>  18
gcacgactgt ctctcccagc                                                   20


<210>  19
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  F3

<400>  19
gcaatagacc gcggactc                                                     18


<210>  20
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RPA_F

<400>  20
ccgcggactc gcgaacgaac aatactaagg                                        30


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  qPCR_primer_F

<400>  21
ggactcgcga acgaacaata                                                   20


<210>  22
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  F2

<400>  22
actaaggggg ccttagcg                                                     18


<210>  23
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  stop codons

<400>  23
ttaattaatt aa                                                           12


<210>  24
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe_qPCR

<400>  24
actggaagct tcgccagtta ttccc                                             25


<210>  25
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  F1c

<400>  25
ccccctatcg aaagagtcgc ag                                                22


<210>  26
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RPA_R (5' - 3')

<400>  26
tttcacgaaa taggcaattg tggctgcgac                                        30


<210>  27
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  qPCR_primer_R (5' - 3')

<400>  27
attgtggctg cgactcttt                                                    19


<210>  28
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B1c

<400>  28
ttcgtgaaag ggatcagagc cc                                                22


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequencing primer_R (5' - 3')

<400>  29
ttgacccgta caggcgaggt                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B2

<400>  30
cctgtacggg tcaatacgga                                                   20


<210>  31
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B3

<400>  31
ccaattgttg cgacaaggc                                                    19


<210>  32
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  stop codons

<400>  32
ttaattaatt aa                                                           12


<210>  33
<211>  291
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  33
ttttgaggga atattcaact gttttttttt atcatgttga tgctctgcat aataatgccc       60

ataaatattt ccgacctgct tttatatctt tgctagccaa actaactgaa catagctaca      120

cattattttc agcttggcta ttttgtgaac actgtatagc cagtccttcg gatcacggtc      180

aacagttgtc cgagcgcttt ttggaccctt tcccttattt ttgggttaag gaaaatgaca      240

gaaaatatat ctaatgagcc ttcgctcaac agtgctccga agtatagctt t               291


<210>  34
<211>  6
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  34
ccaaaa                                                                   6


<210>  35
<211>  137
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  35
ggagaggcaa agcaatttaa gaatgtatga acaaaataaa ggggaaaaat taccccctct       60

actttaccaa acgaatacta ccaataatat ttacaacttt tccttatgat tttttcactg      120

aagcgcttcg caatagt                                                     137


<210>  36
<211>  177
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  36
gccagcaaaa agttggttgc ctttttttac ctaaaaaaga cacatctaac tgattagttt       60

tccgttttag gatattgacg ccaagcgtgc gtctgatttc tacgatatgt ctctaatttt      120

ggaagggcca ttttattttt gaggattggg ctgatcattt tccttacgtg gattgag         177


<210>  37
<211>  24
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  37
ttacgtggat tgagccagca atac                                              24


<210>  38
<211>  310
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  38
ccagcaatac agatcattat taaactgttt tgtacatgat gttagtatat aatcgtaaag       60

cttttctaat atgtatacct tatacatgga actccacaga acttgcaaac ataccaaaaa      120

tcctttattc ttgttcactc attttacatc aaaaaataat atttcagtta ttaaggaaaa      180

taaaaaaata gattagagaa gcattttgaa gaaatagtat attcttttat tgaacctaag      240

agcgtgatat ttttactcga aataaaatac gaaaaatcta tacactcatc tttccgacta      300

ctattggctc                                                             310


<210>  39
<211>  250
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  39
taatttgtca tttgactgac gaatcgttag gcacagttat atagttatat acacatagac       60

atacatatgt tcgcatatat attatattac tatgcattca cctaaatagc tactgctcta      120

ttaatacagt gctttttcga tggctttaat ttaacttatt agacattact ttgcgtttat      180

tcccctccaa tatattttta tgtttgtttt tcttgaacat tttatgattt cttgctcttg      240

gcggctactg                                                             250


<210>  40
<211>  24
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  40
ctttatgaaa atttaacgag gttg                                              24


<210>  41
<211>  250
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  41
ctcgattttg gtattgcacg gctatacaca tctaagaaac tttgtataaa aggtggctac       60

cctattcata gcttgatatc aataggccat ctcatcactt tttattgaaa aggaaaggag      120

ggaaatatat ctgattcaaa ttacttgttt gcttctcttt aagacaaaag catagataat      180

ttcagcgtgg aacgccggaa taagattggt accctcgtca gaaagttaca aataccgctt      240

catcttcaaa                                                             250


