                         SEQUENCE LISTING

<110>  Transmab Pty Ltd
 
<120>  Improved Immunoglobulin II

<130>  534367PRV

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3E10 VH CDR1

<400>  1

Asp Tyr Gly Met His 
1               5   


<210>  2
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3E10 VH CDR2

<400>  2

Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Thr Val Lys 
1               5                   10                  15      


Gly Arg 
        


<210>  3
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3E10 VH CDR3

<400>  3

Arg Gly Leu Leu Leu Asp Tyr 
1               5           


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3E10 VL CDR1

<400>  4

Ser Tyr Met His Trp 
1               5   


<210>  5
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3E10 VL CDR2

<400>  5

Ala Ser Tyr Leu Glu Ser 
1               5       


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3E10 VL CDR3

<400>  6

Gln His Ser Arg Glu Phe Pro Trp Thr 
1               5                   


<210>  7
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3E10 VH

<400>  7

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Arg Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Glu Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Thr Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Phe 
65                  70                  75                  80  


Leu Gln Met Thr Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Gly Leu Leu Leu Asp Tyr Trp Gly Gln Gly Thr Thr Leu 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  8
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3E10 VL

<400>  8

Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Lys Ser Val Ser Thr Ser 
            20                  25                  30          


Ser Tyr Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Lys Tyr Ala Ser Tyr Leu Glu Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln His Ser Arg 
                85                  90                  95      


Glu Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105                 110         


Ala Asp Ala Ala Pro 
        115         


<210>  9
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR1 of DX1/DX3

<400>  9

Gly Phe Thr Phe Ser Asn Tyr Gly Met His 
1               5                   10  


<210>  10
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR1 variant of DX3

<400>  10

Gly Phe Thr Phe Ser Asn Tyr Gly Met Asn 
1               5                   10  


<210>  11
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR2 of DX1/DX3

<400>  11

Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  12
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR2 variant of DX3

<400>  12

Tyr Ile Ser Asn Thr Ser Ser Thr Ile Tyr Tyr Ala Asp Ala Arg Lys 
1               5                   10                  15      


Gly 
    


<210>  13
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR3 of DX1/DX3

<400>  13

Ala Arg Arg Gly Leu Leu Leu Asp Tyr 
1               5                   


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR1 of DX1/DX3

<400>  14

Arg Ala Ser Lys Thr Val Ser Thr Ser Ser Tyr Ser Tyr Met His 
1               5                   10                  15  


<210>  15
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR1 variant of DX3

<400>  15

Arg Ala Ser Lys Thr Val Ser Thr Ser Ser Tyr Gly Tyr Met His 
1               5                   10                  15  


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR2 of DX1/DX3

<400>  16

Tyr Ala Ser Tyr Leu Glu Ser 
1               5           


<210>  17
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR3 of DX1/DX3

<400>  17

Gln His Ser Arg Glu Phe Pro Trp Thr 
1               5                   


<210>  18
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of DX1/DX3

<400>  18

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Gly Leu Leu Leu Asp Tyr Trp Gly Gln Gly Thr Thr Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  19
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of DX3 variant 1

<400>  19

Glu Val Gln Leu Ala Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Tyr Thr Ile Ser Arg Glu Asn Ala Lys Asn Ser Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Gly Leu Leu Leu Asp Tyr Trp Gly Gln Gly Ser Asn Ile 
            100                 105                 110         


Asn Ile Ser Ser 
        115     


<210>  20
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of DX3 variant 2

<400>  20

Glu Val Gln Leu Ala Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Tyr Thr Ile Ser Arg Glu Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Gly Leu Leu Leu Asp Tyr Trp Gly Gln Gly Ser Asn Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  21
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of DX3 variant 3

<400>  21

Glu Val Gln Leu Ala Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Tyr Ile Ser Asn Thr Ser Ser Thr Ile Tyr Tyr Ala Asp Ala Arg 
    50                  55                  60                  


Lys Gly Arg Tyr Thr Ile Ser Arg Glu Asn Ala Lys Asn Ser Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Gly Leu Leu Leu Asp Tyr Trp Gly Gln Gly Ser Asn Ile 
            100                 105                 110         


Asn Ile Ser Ser 
        115     


<210>  22
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of DX1/DX3

<400>  22

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Ala Thr Ile Thr Cys Arg Ala Ser Lys Thr Val Ser Thr Ser 
            20                  25                  30          


Ser Tyr Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Lys Tyr Ala Ser Tyr Leu Glu Ser Gly Val Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Ala Ala Thr Tyr Tyr Cys Gln His Ser Arg 
                85                  90                  95      


Glu Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  23
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of DX3 variant 1

<400>  23

Asp Ile Gln Met Thr His Ser Pro Ala Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Ala Thr Ile Thr Cys Arg Ala Ser Lys Thr Val Ser Thr Ser 
            20                  25                  30          


Ser Tyr Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Lys Tyr Ala Ser Tyr Leu Glu Ser Gly Val Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Gln Ile Asn 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Ala Ala Thr Tyr Tyr Cys Gln His Ser Arg 
                85                  90                  95      


Glu Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  24
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of DX3 variant 2

<400>  24

Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Ala Thr Ile Thr Cys Arg Ala Ser Lys Thr Val Ser Thr Ser 
            20                  25                  30          


Ser Tyr Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Lys Tyr Ala Ser Tyr Leu Glu Ser Gly Val Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Gln Ile Asn 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Ala Ala Asn Tyr Tyr Cys Gln His Ser Arg 
                85                  90                  95      


Glu Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  25
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of DX3 variant 3

<400>  25

Asp Ile Gln Met Thr His Ser Pro Ala Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Ala Thr Ile Thr Cys Arg Ala Ser Lys Thr Val Ser Thr Ser 
            20                  25                  30          


Ser Tyr Gly Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Lys Tyr Ala Ser Tyr Leu Glu Ser Gly Val Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Gln Ile Asn 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Ala Ala Asn Tyr Tyr Cys Gln His Ser Arg 
                85                  90                  95      


Glu Phe Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  26
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 1

<400>  26

Thr Val Asp Ala Ala Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Gly Ser 
            20      


<210>  27
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 2

<400>  27

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Leu Glu Ser 
1               5                   10                  15      


Thr Gly Ser 
            


<210>  28
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge sequence

<400>  28

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  29
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal sequence

<400>  29

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Phe Arg Gly 
1               5                   10                  15      


Val Gln Cys 
            


<210>  30
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of 5C6

<400>  30

Gln Leu Lys Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Thr Met Ser Trp Val Arg Gln Thr Pro Ala Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser Ser Gly Gly Gly Ser Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Ala Tyr Ser Lys Arg Gly Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser 
        115                 120 


<210>  31
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR1 of 5C6

<400>  31

Ser Tyr Thr Met Ser 
1               5   


<210>  32
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR2 of 5C6

<400>  32

Thr Ile Ser Ser Gly Gly Gly Ser Thr Tyr Tyr Pro Asp Ser Val Lys 
1               5                   10                  15      


<210>  33
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR3 of 5C6

<400>  33

Arg Ala Tyr Ser Lys Arg Gly Ala Met Asp Tyr 
1               5                   10      


<210>  34
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of 5C6

<400>  34

Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Ala Val Ser Leu 
1               5                   10                  15      


Gly Glu Arg Ala Thr Ile Ser Tyr Arg Ala Ser Lys Ser Val Ser Thr 
            20                  25                  30          


Ser Gly Tyr Ser Tyr Met His Trp Asn Gln Gln Lys Pro Gly Gln Ala 
        35                  40                  45              


Pro Arg Leu Leu Ile Tyr Leu Val Ser Asn Leu Glu Ser Gly Val Pro 
    50                  55                  60                  


Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile 
65                  70                  75                  80  


His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln His Ile 
                85                  90                  95      


Arg Glu Leu Asp Thr Phe Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  35
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR1 of 5C6

<400>  35

Arg Ala Ser Lys Ser Val Ser Thr Ser Gly Tyr Ser Tyr Met His 
1               5                   10                  15  


<210>  36
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR2 of 5C6

<400>  36

Leu Val Ser Asn Leu Glu Ser 
1               5           


<210>  37
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR3 of 5C6

<400>  37

Gln His Ile Arg Glu Leu Asp Thr Phe 
1               5                   


