                         SEQUENCE LISTING

<110>  Transmab Pty Ltd
 
<120>  Improved Immunoglobulin I

<130>  534366PRV

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR1

<400>  1

Gly Phe Thr Phe Ser Asn Tyr Gly Met His 
1               5                   10  


<210>  2
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR2

<400>  2

Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR3

<400>  3

Ala Arg Arg Gly Leu Leu Leu Asp Tyr 
1               5                   


<210>  4
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR1

<400>  4

Arg Ala Ser Lys Thr Val Ser Thr Ser Ser Tyr Ser Tyr Met His 
1               5                   10                  15  


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR2

<400>  5

Tyr Ala Ser Tyr Leu Glu Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR3

<400>  6

Gln His Ser Arg Glu Phe Pro Trp Thr 
1               5                   


<210>  7
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH

<400>  7

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Gly Leu Leu Leu Asp Tyr Trp Gly Gln Gly Thr Thr Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  8
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL

<400>  8

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Ala Thr Ile Thr Cys Arg Ala Ser Lys Thr Val Ser Thr Ser 
            20                  25                  30          


Ser Tyr Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Lys Tyr Ala Ser Tyr Leu Glu Ser Gly Val Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Ala Ala Thr Tyr Tyr Cys Gln His Ser Arg 
                85                  90                  95      


Glu Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  9
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Full heavy chain of DX3

<400>  9

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Gly Leu Leu Leu Asp Tyr Trp Gly Gln Gly Thr Thr Val 
            100                 105                 110         


Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 
        115                 120                 125             


Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 
    130                 135                 140                 


Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 
145                 150                 155                 160 


Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 
                165                 170                 175     


Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 
            180                 185                 190         


Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 
        195                 200                 205             


Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
    210                 215                 220                 


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
225                 230                 235                 240 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
                245                 250                 255     


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
            260                 265                 270         


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        275                 280                 285             


Lys Pro Arg Glu Glu Gln Tyr Asp Ser Thr Tyr Arg Val Val Ser Val 
    290                 295                 300                 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
305                 310                 315                 320 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
                325                 330                 335     


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            340                 345                 350         


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        355                 360                 365             


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    370                 375                 380                 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
385                 390                 395                 400 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
                405                 410                 415     


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            420                 425                 430         


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445     


<210>  10
<211>  218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fulll light chain of DX3

<400>  10

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Ala Thr Ile Thr Cys Arg Ala Ser Lys Thr Val Ser Thr Ser 
            20                  25                  30          


Ser Tyr Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Lys Tyr Ala Ser Tyr Leu Glu Ser Gly Val Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Ala Ala Thr Tyr Tyr Cys Gln His Ser Arg 
                85                  90                  95      


Glu Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
        115                 120                 125             


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
    130                 135                 140                 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
145                 150                 155                 160 


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
                165                 170                 175     


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
            180                 185                 190         


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
        195                 200                 205             


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215             


<210>  11
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain CDR1 variant of DX3

<400>  11

Arg Ala Ser Lys Thr Val Ser Thr Ser Ser Tyr Gly Tyr Met His 
1               5                   10                  15  


<210>  12
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain CDR1 variant of DX3

<400>  12

Gly Phe Thr Phe Ser Asn Tyr Gly Met Asn 
1               5                   10  


<210>  13
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain CDR2 variant of DX3

<400>  13

Tyr Ile Ser Asn Thr Ser Ser Thr Ile Tyr Tyr Ala Asp Ala Arg Lys 
1               5                   10                  15      


Gly 
    


<210>  14
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain (VL) of DX3 variant 1

<400>  14

Asp Ile Gln Met Thr His Ser Pro Ala Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Ala Thr Ile Thr Cys Arg Ala Ser Lys Thr Val Ser Thr Ser 
            20                  25                  30          


Ser Tyr Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Lys Tyr Ala Ser Tyr Leu Glu Ser Gly Val Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Gln Ile Asn 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Ala Ala Thr Tyr Tyr Cys Gln His Ser Arg 
                85                  90                  95      


Glu Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  15
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain (VL) of DX3 variant 2

<400>  15

Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Ala Thr Ile Thr Cys Arg Ala Ser Lys Thr Val Ser Thr Ser 
            20                  25                  30          


Ser Tyr Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Lys Tyr Ala Ser Tyr Leu Glu Ser Gly Val Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Gln Ile Asn 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Ala Ala Asn Tyr Tyr Cys Gln His Ser Arg 
                85                  90                  95      


Glu Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  16
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain (VL) of DX3 variant 3

<400>  16

Asp Ile Gln Met Thr His Ser Pro Ala Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Ala Thr Ile Thr Cys Arg Ala Ser Lys Thr Val Ser Thr Ser 
            20                  25                  30          


Ser Tyr Gly Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Lys Tyr Ala Ser Tyr Leu Glu Ser Gly Val Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Gln Ile Asn 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Ala Ala Asn Tyr Tyr Cys Gln His Ser Arg 
                85                  90                  95      


Glu Phe Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  17
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain (VH) of DX3 variant 1

<400>  17

Glu Val Gln Leu Ala Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Tyr Thr Ile Ser Arg Glu Asn Ala Lys Asn Ser Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Gly Leu Leu Leu Asp Tyr Trp Gly Gln Gly Ser Asn Ile 
            100                 105                 110         


Asn Ile Ser Ser 
        115     


<210>  18
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain (VH) of DX3 variant 2

<400>  18

Glu Val Gln Leu Ala Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Tyr Thr Ile Ser Arg Glu Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Gly Leu Leu Leu Asp Tyr Trp Gly Gln Gly Ser Asn Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  19
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain (VH) of DX3 variant 3

<400>  19

Glu Val Gln Leu Ala Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Tyr Ile Ser Asn Thr Ser Ser Thr Ile Tyr Tyr Ala Asp Ala Arg 
    50                  55                  60                  


Lys Gly Arg Tyr Thr Ile Ser Arg Glu Asn Ala Lys Asn Ser Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Gly Leu Leu Leu Asp Tyr Trp Gly Gln Gly Ser Asn Ile 
            100                 105                 110         


Asn Ile Ser Ser 
        115     


<210>  20
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge sequence

<400>  20

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  21
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal sequence

<400>  21

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Phe Arg Gly 
1               5                   10                  15      


Val Gln Cys 
            


