                         SEQUENCE LISTING

<110>  Oxford University Innovation Limited
 
<120>  POLYPEPTIDES RELATED TO HMGB1 USEFUL FOR PROMOTING TISSUE 
       REGENERATION, COMPSITIONS COMPRISING SAME, AND USES THEREOF

<130>  91203-B-PCT/JPW/AWG

<150>  63/190,429
<151>  2021-05-19

<160>  178   

<170>  PatentIn version 3.5

<210>  1
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  WT HMGB1

<400>  1

Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr Ala 
1               5                   10                  15      


Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro Asp 
            20                  25                  30          


Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg Trp 
        35                  40                  45              


Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala Lys 
    50                  55                  60                  


Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro Pro 
65                  70                  75                  80  


Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys Arg 
                85                  90                  95      


Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys Ile 
            100                 105                 110         


Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys Leu 
        115                 120                 125             


Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr Glu 
    130                 135                 140                 


Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala Ala 
145                 150                 155                 160 


Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val Val Lys 
                165                 170                 175     


Ala Glu Lys Ser Lys Lys Lys Lys Glu Glu Glu Glu Asp Glu Glu Asp 
            180                 185                 190         


Glu Glu Asp Glu Glu Glu Glu Glu Asp Glu Glu Asp Glu Asp Glu Glu 
        195                 200                 205             


Glu Asp Asp Asp Asp Glu 
    210                 


<210>  2
<211>  172
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Dbb12L

<400>  2

Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe 
1               5                   10                  15      


Cys Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser 
            20                  25                  30          


Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala 
        35                  40                  45              


Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu 
    50                  55                  60                  


Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp 
65                  70                  75                  80  


Ala Ala Lys Lys Gly Val Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro 
                85                  90                  95      


Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys Ile Lys Gly 
            100                 105                 110         


Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys Leu Gly Glu 
        115                 120                 125             


Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys 
    130                 135                 140                 


Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg 
145                 150                 155                 160 


Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val 
                165                 170         


<210>  3
<211>  89
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 portion containing Box A shown in alignment

<400>  3

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe 
                85                  


<210>  4
<211>  86
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 portion containing Box B shown in alignment

<400>  4

Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe 
1               5                   10                  15      


Cys Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser 
            20                  25                  30          


Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala 
        35                  40                  45              


Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu 
    50                  55                  60                  


Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp 
65                  70                  75                  80  


Ala Ala Lys Lys Gly Val 
                85      


<210>  5
<211>  85
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 Box B

<400>  5

Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys 
1               5                   10                  15      


Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser Ile 
            20                  25                  30          


Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala Ala 
        35                  40                  45              


Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys 
    50                  55                  60                  


Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala 
65                  70                  75                  80  


Ala Lys Lys Gly Val 
                85  


<210>  6
<211>  84
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 3S Box B

<400>  6

Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe Ser 
1               5                   10                  15      


Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser Ile 
            20                  25                  30          


Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala Ala 
        35                  40                  45              


Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys 
    50                  55                  60                  


Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala 
65                  70                  75                  80  


Lys Lys Gly Val 
                


<210>  7
<211>  85
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 3S Box A

<400>  7

Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr Ala Phe Phe Val 
1               5                   10                  15      


Gln Thr Ser Arg Glu Glu His Lys Lys Lys His Pro Asp Ala Ser Val 
            20                  25                  30          


Asn Phe Ser Glu Phe Ser Lys Lys Ser Ser Glu Arg Trp Lys Thr Met 
        35                  40                  45              


Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala Lys Ala Asp Lys 
    50                  55                  60                  


Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro Pro Lys Gly Glu 
65                  70                  75                  80  


Thr Lys Lys Lys Phe 
                85  


<210>  8
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  8

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser 
1               5                   10                  15  


<210>  9
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  9

Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr Ala 
1               5                   10                  15  


<210>  10
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  10

Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr Ala Phe Phe 
1               5                   10                  15  


<210>  11
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  11

Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr Ala Phe Phe Val Gln 
1               5                   10                  15  


<210>  12
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  12

Pro Arg Gly Lys Met Ser Ser Tyr Ala Phe Phe Val Gln Thr Cys 
1               5                   10                  15  


<210>  13
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  13

Gly Lys Met Ser Ser Tyr Ala Phe Phe Val Gln Thr Cys Arg Glu 
1               5                   10                  15  


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  14

Met Ser Ser Tyr Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His 
1               5                   10                  15  


<210>  15
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  15

Ser Tyr Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys 
1               5                   10                  15  


<210>  16
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  16

Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His 
1               5                   10                  15  


<210>  17
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  17

Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro Asp 
1               5                   10                  15  


<210>  18
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  18

Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro Asp Ala Ser 
1               5                   10                  15  


<210>  19
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  19

Cys Arg Glu Glu His Lys Lys Lys His Pro Asp Ala Ser Val Asn 
1               5                   10                  15  


<210>  20
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  20

Glu Glu His Lys Lys Lys His Pro Asp Ala Ser Val Asn Phe Ser 
1               5                   10                  15  


<210>  21
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  21

His Lys Lys Lys His Pro Asp Ala Ser Val Asn Phe Ser Glu Phe 
1               5                   10                  15  


<210>  22
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  22

Lys Lys His Pro Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys 
1               5                   10                  15  


<210>  23
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  23

His Pro Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys 
1               5                   10                  15  


<210>  24
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  24

Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu 
1               5                   10                  15  


<210>  25
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  25

Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg Trp 
1               5                   10                  15  


<210>  26
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  26

Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg Trp Lys Thr 
1               5                   10                  15  


<210>  27
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  27

Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg Trp Lys Thr Met Ser 
1               5                   10                  15  


<210>  28
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  28

Phe Ser Lys Lys Cys Ser Glu Arg Trp Lys Thr Met Ser Ala Lys 
1               5                   10                  15  


<210>  29
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  29

Lys Lys Cys Ser Glu Arg Trp Lys Thr Met Ser Ala Lys Glu Lys 
1               5                   10                  15  


<210>  30
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  30

Cys Ser Glu Arg Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys 
1               5                   10                  15  


<210>  31
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  31

Glu Arg Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu 
1               5                   10                  15  


<210>  32
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  32

Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met 
1               5                   10                  15  


<210>  33
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  33

Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala Lys 
1               5                   10                  15  


<210>  34
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  34

Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala Lys Ala Asp 
1               5                   10                  15  


<210>  35
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  35

Lys Glu Lys Gly Lys Phe Glu Asp Met Ala Lys Ala Asp Lys Ala 
1               5                   10                  15  


<210>  36
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  36

Lys Gly Lys Phe Glu Asp Met Ala Lys Ala Asp Lys Ala Arg Tyr 
1               5                   10                  15  


<210>  37
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  37

Lys Phe Glu Asp Met Ala Lys Ala Asp Lys Ala Arg Tyr Glu Arg 
1               5                   10                  15  


<210>  38
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  38

Glu Asp Met Ala Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met 
1               5                   10                  15  


<210>  39
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  39

Met Ala Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr 
1               5                   10                  15  


<210>  40
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  40

Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile 
1               5                   10                  15  


<210>  41
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  41

Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro Pro 
1               5                   10                  15  


<210>  42
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  42

Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro Pro Lys Gly 
1               5                   10                  15  


<210>  43
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  43

Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro Pro Lys Gly Glu Thr 
1               5                   10                  15  


<210>  44
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  44

Arg Glu Met Lys Thr Tyr Ile Pro Pro Lys Gly Glu Thr Lys Lys 
1               5                   10                  15  


<210>  45
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  45

Met Lys Thr Tyr Ile Pro Pro Lys Gly Glu Thr Lys Lys Lys Phe 
1               5                   10                  15  


<210>  46
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  46

Thr Tyr Ile Pro Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp 
1               5                   10                  15  


<210>  47
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  47

Ile Pro Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn 
1               5                   10                  15  


<210>  48
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  48

Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro 
1               5                   10                  15  


<210>  49
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  49

Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys Arg 
1               5                   10                  15  


<210>  50
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  50

Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro 
1               5                   10                  15  


<210>  51
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  51

Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala 
1               5                   10                  15  


<210>  52
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  52

Phe Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe 
1               5                   10                  15  


<210>  53
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  53

Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe 
1               5                   10                  15  


<210>  54
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  54

Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser 
1               5                   10                  15  


<210>  55
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  55

Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr 
1               5                   10                  15  


<210>  56
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  56

Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro 
1               5                   10                  15  


<210>  57
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  57

Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys Ile 
1               5                   10                  15  


<210>  58
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  58

Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys Ile Lys Gly 
1               5                   10                  15  


<210>  59
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  59

Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His 
1               5                   10                  15  


<210>  60
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  60

Phe Cys Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly 
1               5                   10                  15  


<210>  61
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  61

Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser 
1               5                   10                  15  


<210>  62
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  62

Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly 
1               5                   10                  15  


<210>  63
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  63

Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val 
1               5                   10                  15  


<210>  64
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  64

Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys 
1               5                   10                  15  


<210>  65
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  65

Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys Leu 
1               5                   10                  15  


<210>  66
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  66

His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys Leu Gly Glu 
1               5                   10                  15  


<210>  67
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  67

Gly Leu Ser Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp 
1               5                   10                  15  


<210>  68
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  68

Ser Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn 
1               5                   10                  15  


<210>  69
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  69

Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala 
1               5                   10                  15  


<210>  70
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  70

Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp 
1               5                   10                  15  


<210>  71
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  71

Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys 
1               5                   10                  15  


<210>  72
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  72

Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro 
1               5                   10                  15  


<210>  73
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  73

Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr Glu 
1               5                   10                  15  


<210>  74
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  74

Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys 
1               5                   10                  15  


<210>  75
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  75

Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala 
1               5                   10                  15  


<210>  76
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  76

Ala Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu 
1               5                   10                  15  


<210>  77
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  77

Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu 
1               5                   10                  15  


<210>  78
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  78

Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr 
1               5                   10                  15  


<210>  79
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  79

Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys 
1               5                   10                  15  


<210>  80
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  80

Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile 
1               5                   10                  15  


<210>  81
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  81

Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala Ala 
1               5                   10                  15  


<210>  82
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  82

Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg 
1               5                   10                  15  


<210>  83
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  83

Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys 
1               5                   10                  15  


<210>  84
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  84

Glu Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys 
1               5                   10                  15  


<210>  85
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  85

Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp 
1               5                   10                  15  


<210>  86
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  86

Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala 
1               5                   10                  15  


<210>  87
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  87

Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys 
1               5                   10                  15  


<210>  88
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  88

Asp Ala Ala Lys Lys Gly Val Val Lys Ala Glu Lys Ser Lys Lys 
1               5                   10                  15  


<210>  89
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  89

Ala Lys Lys Gly Val Val Lys Ala Glu Lys Ser Lys Lys Lys Lys 
1               5                   10                  15  


<210>  90
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  90

Lys Gly Val Val Lys Ala Glu Lys Ser Lys Lys Lys Lys Glu Glu 
1               5                   10                  15  


<210>  91
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  91

Val Val Lys Ala Glu Lys Ser Lys Lys Lys Lys Glu Glu Glu Glu 
1               5                   10                  15  


<210>  92
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  92

Lys Ala Glu Lys Ser Lys Lys Lys Lys Glu Glu Glu Glu Asp Glu 
1               5                   10                  15  


<210>  93
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  93

Glu Lys Ser Lys Lys Lys Lys Glu Glu Glu Glu Asp Glu Glu Asp 
1               5                   10                  15  


<210>  94
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  94

Ser Lys Lys Lys Lys Glu Glu Glu Glu Asp Glu Glu Asp Glu Glu 
1               5                   10                  15  


<210>  95
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  95

Lys Lys Lys Glu Glu Glu Glu Asp Glu Glu Asp Glu Glu Asp Glu 
1               5                   10                  15  


<210>  96
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  96

Lys Glu Glu Glu Glu Asp Glu Glu Asp Glu Glu Asp Glu Glu Glu 
1               5                   10                  15  


<210>  97
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  97

Glu Glu Glu Asp Glu Glu Asp Glu Glu Asp Glu Glu Glu Glu Glu 
1               5                   10                  15  


<210>  98
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  98

Glu Asp Glu Glu Asp Glu Glu Asp Glu Glu Glu Glu Glu Asp Glu 
1               5                   10                  15  


<210>  99
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  99

Glu Glu Asp Glu Glu Asp Glu Glu Glu Glu Glu Asp Glu Glu Asp 
1               5                   10                  15  


<210>  100
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  100

Asp Glu Glu Asp Glu Glu Glu Glu Glu Asp Glu Glu Asp Glu Asp 
1               5                   10                  15  


<210>  101
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  101

Glu Asp Glu Glu Glu Glu Glu Asp Glu Glu Asp Glu Asp Glu Glu 
1               5                   10                  15  


<210>  102
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  102

Glu Glu Glu Glu Glu Asp Glu Glu Asp Glu Asp Glu Glu Glu Asp 
1               5                   10                  15  


<210>  103
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  103

Glu Glu Glu Asp Glu Glu Asp Glu Asp Glu Glu Glu Asp Asp Asp 
1               5                   10                  15  


<210>  104
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  104

Glu Asp Glu Glu Asp Glu Asp Glu Glu Glu Asp Asp Asp Asp Glu 
1               5                   10                  15  


<210>  105
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  105

Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr Ala Phe Phe Val 
1               5                   10                  15      


Gln 
    


<210>  106
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  106

Met Ser Ser Tyr Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His 
1               5                   10                  15  


<210>  107
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  107

His Lys Lys Lys His Pro Asp Ala Ser Val Asn Phe Ser Glu Phe 
1               5                   10                  15  


<210>  108
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  108

Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
1               5                   10                  15      


Pro Lys Gly 
            


<210>  109
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  109

Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys 
1               5                   10                  15      


Ser Glu Tyr 
            


<210>  110
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  110

Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly 
1               5                   10                  15  


<210>  111
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 peptide

<400>  111

Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly 
1               5                   10                  15      


Lys Pro Asp 
            


<210>  112
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-214 (HMGB1A-s001) pNIC-CTHF Forward LIC cloning primer

<400>  112
ttaagaagga gatatactat gggcaaagga gatcctaaga agccg                       45


<210>  113
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-214 (HMGB1A-s001) pNIC-CTHF Reverse LIC cloning primer

<400>  113
ttaagaagga gatatactat gggcaaagga gatcctaaga agccg                       45


<210>  114
<211>  645
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-214 (HMGB1A-s001) pNIC-CTHF DNA sequence cloned into 
       the vector

<400>  114
atgggcaaag gagatcctaa gaagccgaga ggcaaaatgt catcatatgc attttttgtg       60

caaacttgtc gggaggagca taagaagaag cacccagatg cttcagtcaa cttctcagag      120

ttttctaaga agtgctcaga gaggtggaag accatgtctg ctaaagagaa aggaaaattt      180

gaagatatgg caaaagcgga caaggcccgt tatgaaagag aaatgaaaac ctatatccct      240

cccaaagggg agacaaaaaa gaagttcaag gatcccaatg cacccaagag gcctccttcg      300

gccttcttcc tcttctgctc tgagtatcgc ccaaaaatca aaggagaaca tcctggcctg      360

tccattggtg atgttgcgaa gaaactggga gagatgtgga ataacactgc tgcagatgac      420

aagcagcctt atgaaaagaa ggctgcgaag ctgaaggaaa aatacgaaaa ggatattgct      480

gcatatcgag ctaaaggaaa gcctgatgca gcaaaaaagg gagttgtcaa ggctgaaaaa      540

agcaagaaaa agaaggaaga ggaggaagat gaggaagatg aagaggatga ggaggaggag      600

gaagatgaag aagatgaaga tgaagaagaa gatgatgatg atgaa                      645


<210>  115
<211>  522
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dBB12L pNIC-CTHF DNA sequence cloned into the vector

<400>  115
atgaaggacc cgaacgcgcc gaagcgccca ccgtccgctt tttttctgtt ctgtagcgaa       60

taccgtccaa aaattaaagg tgaacaccca ggcctttcta tcggcgatgt tgcgaaaaaa      120

ctgggtgaga tgtggaacaa tactgcagca gatgataaac aaccgtatga aaagaaagcg      180

gcgaagctga aagaaaaata cgaaaaagat atcgctgcgt atcgggccaa ggggaaaccg      240

gatgcggcga aaaaaggcgt caaggatccc aacgcgccga aacgtccacc gagcgcgttc      300

tttctgtttt gttcagaata ccgcccgaaa attaaaggcg aacacccggg cctttcgatt      360

ggtgacgttg cgaaaaaact tggcgaaatg tggaataaca cggcagcgga cgataagcag      420

ccttatgaaa aaaaagccgc caaacttaaa gaaaaatacg agaaagacat tgcagcttac      480

cgcgcgaagg gtaaacctga cgctgcaaaa aaaggtgttt aa                         522


<210>  116
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3S-HMGB1 1-214 (HMGB1A-s002) pNIC-CTHF Forward LIC cloning primer

<400>  116
ttaagaagga gatatactat gggcaaagga gatcctaaga agccg                       45


<210>  117
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3S-HMGB1 1-214 (HMGB1A-s002) pNIC-CTHF Reverse LIC cloning primer

<400>  117
ttaagaagga gatatactat gggcaaagga gatcctaaga agccg                       45


<210>  118
<211>  645
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3S-HMGB1 1-214 (HMGB1A-s002) pNIC-CTHF DNA sequence cloned into 
       the vector

<400>  118
atgggcaaag gagatcctaa gaagccgaga ggcaaaatgt catcatatgc attttttgtg       60

caaactagtc gggaggagca taagaagaag cacccagatg cttcagtcaa cttctcagag      120

ttttctaaga agagctcaga gaggtggaag accatgtctg ctaaagagaa aggaaaattt      180

gaagatatgg caaaagcgga caaggcccgt tatgaaagag aaatgaaaac ctatatccct      240

cccaaagggg agacaaaaaa gaagttcaag gatcccaatg cacccaagag gcctccttcg      300

gccttcttcc tcttcagctc tgagtatcgc ccaaaaatca aaggagaaca tcctggcctg      360

tccattggtg atgttgcgaa gaaactggga gagatgtgga ataacactgc tgcagatgac      420

aagcagcctt atgaaaagaa ggctgcgaag ctgaaggaaa aatacgaaaa ggatattgct      480

gcatatcgag ctaaaggaaa gcctgatgca gcaaaaaagg gagttgtcaa ggctgaaaaa      540

agcaagaaaa agaaggaaga ggaggaagat gaggaagatg aagaggatga ggaggaggag      600

gaagatgaag aagatgaaga tgaagaagaa gatgatgatg atgaa                      645


<210>  119
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-184 pNIC-CTHF Forward LIC cloning primer

<400>  119
ttaagaagga gatatactat gggcaaagga gatcctaaga agccg                       45


<210>  120
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-184 pNIC-CTHF Reverse LIC cloning primer

<400>  120
tatccacctt tactgctttc atcatcatca tcttcttctt catcttcatc                  50


<210>  121
<211>  555
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-184 pNIC-CTHF DNA sequence cloned into the vector

<400>  121
atgggcaaag gagatcctaa gaagccgaga ggcaaaatgt catcatatgc attttttgtg       60

caaacttgtc gggaggagca taagaagaag cacccagatg cttcagtcaa cttctcagag      120

ttttctaaga agtgctcaga gaggtggaag accatgtctg ctaaagagaa aggaaaattt      180

gaagatatgg caaaagcgga caaggcccgt tatgaaagag aaatgaaaac ctatatccct      240

cccaaagggg agacaaaaaa gaagttcaag gatcccaatg cacccaagag gcctccttcg      300

gccttcttcc tcttctgctc tgagtatcgc ccaaaaatca aaggagaaca tcctggcctg      360

tccattggtg atgttgcgaa gaaactggga gagatgtgga ataacactgc tgcagatgac      420

aagcagcctt atgaaaagaa ggctgcgaag ctgaaggaaa aatacgaaaa ggatattgct      480

gcatatcgag ctaaaggaaa gcctgatgca gcaaaaaagg gagttgtcaa ggctgaaaaa      540

agcaagaaaa agaag                                                       555


<210>  122
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-164 pNIC-CTHF Forward LIC cloning primer

<400>  122
ttaagaagga gatatactat gggcaaagga gatcctaaga agccg                       45


<210>  123
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-164 pNIC-CTHF Reverse LIC cloning primer

<400>  123
tacttccaat ccatgccgag aggcaaaatg tca                                    33


<210>  124
<211>  495
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-164 pNIC-CTHF DNA sequence cloned into the vector

<400>  124
atgggcaaag gagatcctaa gaagccgaga ggcaaaatgt catcatatgc attttttgtg       60

caaacttgtc gggaggagca taagaagaag cacccagatg cttcagtcaa cttctcagag      120

ttttctaaga agtgctcaga gaggtggaag accatgtctg ctaaagagaa aggaaaattt      180

gaagatatgg caaaagcgga caaggcccgt tatgaaagag aaatgaaaac ctatatccct      240

cccaaagggg agacaaaaaa gaagttcaag gatcccaatg cacccaagag gcctccttcg      300

gccttcttcc tcttctgctc tgagtatcgc ccaaaaatca aaggagaaca tcctggcctg      360

tccattggtg atgttgcgaa gaaactggga gagatgtgga ataacactgc tgcagatgac      420

aagcagcctt atgaaaagaa ggctgcgaag ctgaaggaaa aatacgaaaa ggatattgct      480

gcatatcgag ctaaa                                                       495


<210>  125
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 1-88, biotinylated pNIC-Bio3 Forward LIC cloning primer

<400>  125
tacttccaat ccatgggcaa aggagatcct aagaagc                                37


<210>  126
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 1-88, biotinylated pNIC-Bio3 Reverse LIC cloning primer

<400>  126
tatccacctt tactgctgaa cttctttttt gtctcccct                              39


<210>  127
<211>  333
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 1-88, biotinylated pNIC-Bio3 DNA sequence cloned into the 
       vector

<400>  127
atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc       60

caatccatgg gcaaaggaga tcctaagaag ccgagaggca aaatgtcatc atatgcattt      120

tttgtgcaaa cttgtcggga ggagcataag aagaagcacc cagatgcttc agtcaacttc      180

tcagagtttt ctaagaagtg ctcagagagg tggaagacca tgtctgctaa agagaaagga      240

aaatttgaag atatggcaaa agcggacaag gcccgttatg aaagagaaat gaaaacctat      300

atccctccca aaggggagac aaaaaagaag ttc                                   333


<210>  128
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 89-174, biotinylated pNIC-Bio3 Forward LIC cloning primer

<400>  128
tacttccaat ccatgaagga tcccaatgca ccc                                    33


<210>  129
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 89-174, biotinylated pNIC-Bio3 Reverse LIC cloning primer

<400>  129
tatccacctt tactgctaac tccctttttt gctgcatca                              39


<210>  130
<211>  327
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 89-174, biotinylated pNIC-Bio3 DNA sequence cloned into the
       vector

<400>  130
atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc       60

caatccatga aggatcccaa tgcacccaag aggcctcctt cggccttctt cctcttctgc      120

tctgagtatc gcccaaaaat caaaggagaa catcctggcc tgtccattgg tgatgttgcg      180

aagaaactgg gagagatgtg gaataacact gctgcagatg acaagcagcc ttatgaaaag      240

aaggctgcga agctgaagga aaaatacgaa aaggatattg ctgcatatcg agctaaagga      300

aagcctgatg cagcaaaaaa gggagtt                                          327


<210>  131
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 1-88 pNIC-CTHF Forward LIC cloning primer

<400>  131
ttaagaagga gatatactat gggcaaagga gatcctaaga agccg                       45


<210>  132
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 1-88 pNIC-CTHF Reverse LIC cloning primer

<400>  132
gattggaagt agaggttctc tgcgaacttc ttttttgtct cccc                        44


<210>  133
<211>  267
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 1-88 pNIC-CTHF DNA sequence cloned into the vector

<400>  133
atgggcaaag gagatcctaa gaagccgaga ggcaaaatgt catcatatgc attttttgtg       60

caaacttgtc gggaggagca taagaagaag cacccagatg cttcagtcaa cttctcagag      120

ttttctaaga agtgctcaga gaggtggaag accatgtctg ctaaagagaa aggaaaattt      180

gaagatatgg caaaagcgga caaggcccgt tatgaaagag aaatgaaaac ctatatccct      240

cccaaagggg agacaaaaaa gaagttc                                          267


<210>  134
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 89-174 pNIC-CTHF Forward LIC cloning primer

<400>  134
ttaagaagga gatatactat gaaggatccc aatgcaccca agag                        44


<210>  135
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 89-174 pNIC-CTHF Reverse LIC cloning primer

<400>  135
gattggaagt agaggttctc tgcaactccc ttttttgctg ca                          42


<210>  136
<211>  261
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 89-174 pNIC-CTHF DNA sequence cloned into the vector

<400>  136
atgaaggatc ccaatgcacc caagaggcct ccttcggcct tcttcctctt ctgctctgag       60

tatcgcccaa aaatcaaagg agaacatcct ggcctgtcca ttggtgatgt tgcgaagaaa      120

ctgggagaga tgtggaataa cactgctgca gatgacaagc agccttatga aaagaaggct      180

gcgaagctga aggaaaaata cgaaaaggat attgctgcat atcgagctaa aggaaagcct      240

gatgcagcaa aaaagggagt t                                                261


<210>  137
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 8-78, biotinylated pNIC-Bio3 Forward LIC cloning primer

<400>  137
tacttccaat ccatgccgag aggcaaaatg tca                                    33


<210>  138
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 8-78, biotinylated pNIC-Bio3 Reverse LIC cloning primer

<400>  138
tatccacctt tactgctgat ataggttttc atttctcttt cataacggg                   49


<210>  139
<211>  282
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 8-78, biotinylated pNIC-Bio3 DNA sequence cloned into the 
       vector

<400>  139
atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc       60

caatccatgc cgagaggcaa aatgtcatca tatgcatttt ttgtgcaaac ttgtcgggag      120

gagcataaga agaagcaccc agatgcttca gtcaacttct cagagttttc taagaagtgc      180

tcagagaggt ggaagaccat gtctgctaaa gagaaaggaa aatttgaaga tatggcaaaa      240

gcggacaagg cccgttatga aagagaaatg aaaacctata tc                         282


<210>  140
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 94-162, biotinylated pNIC-Bio3 Forward LIC cloning primer

<400>  140
tacttccaat ccatgcccaa gaggcctcct tcg                                    33


<210>  141
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 94-162, biotinylated pNIC-Bio3 Reverse LIC cloning primer

<400>  141
tatccacctt tactgcttcg atatgcagca atatcctttt cg                          42


<210>  142
<211>  276
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1 94-162, biotinylated pNIC-Bio3 DNA sequence cloned into the
       vector

<400>  142
atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc       60

caatccatgc ccaagaggcc tccttcggcc ttcttcctct tctgctctga gtatcgccca      120

aaaatcaaag gagaacatcc tggcctgtcc attggtgatg ttgcgaagaa actgggagag      180

atgtggaata acactgctgc agatgacaag cagccttatg aaaagaaggc tgcgaagctg      240

aaggaaaaat acgaaaagga tattgctgca tatcga                                276


<210>  143
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wt CXCL12 with SUMO pDsbC-HT-CBio Forward LIC cloning primer

<400>  143
tacttccaat ccatgtctga ccaggaggca aaaccttcaa ctg                         43


<210>  144
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wt CXCL12 with SUMO pDsbC-HT-CBio Reverse LIC cloning primer

<400>  144
tatccacctt tactgtcatt acttgtttaa agctttctcc aggta                       45


<210>  145
<211>  495
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wt CXCL12 with SUMO pDsbC-HT-CBio DNA sequence cloned into the 
       vector

<400>  145
atgtctgacc aggaggcaaa accttcaact gaggacttgg gggataagaa ggaaggtgaa       60

tatattaaac tcaaagtcat tggacaggat agcagtgaga ttcacttcaa agtgaaaatg      120

acaacacatc tcaagaaact caaagaatca tactgtcaaa gacagggtgt tccaatgaat      180

tcactcaggt ttctctttga gggtcagaga attgctgata atcatactcc aaaagaactg      240

ggaatggagg aagaagatgt gattgaagtt taccaggagc aaacgggagg taagcccgtc      300

agcctgagct acagatgccc atgccgattc ttcgaaagcc atgttgccag agccaacgtc      360

aagcatctca aaattctcaa cactccaaac tgtgcccttc agattgtagc ccggctgaag      420

aacaacaaca gacaagtgtg cattgacccg aagctaaagt ggattcagga gtacctggag      480

aaagctttaa acaag                                                       495


<210>  146
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1WTLICF

<400>  146
atgggcaaag gcgatccgaa aaaaccgc                                          28


<210>  147
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1C106S

<400>  147
gcgatactca gagctgaaga ggaagaaggc                                        30


<210>  148
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1C45S

<400>  148
ttttctaaga agagctcaga gaggtggaa                                         29


<210>  149
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1WTLICR

<400>  149
ttattcatca tcatcatctt cttcttca                                          28


<210>  150
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGB1C23S

<400>  150
tttttgtgca gaccagccgc gaagaaca                                          28


<210>  151
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-214 (HMGB1A-s001) pNIC-CTHF

<400>  151

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys 
                85                  90                  95      


Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys 
            100                 105                 110         


Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys 
        115                 120                 125             


Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr 
    130                 135                 140                 


Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala 
145                 150                 155                 160 


Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val Val 
                165                 170                 175     


Lys Ala Glu Lys Ser Lys Lys Lys Lys Glu Glu Glu Glu Asp Glu Glu 
            180                 185                 190         


Asp Glu Glu Asp Glu Glu Glu Glu Glu Asp Glu Glu Asp Glu Asp Glu 
        195                 200                 205             


Glu Glu Asp Asp Asp Asp Glu 
    210                 215 


<210>  152
<211>  222
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-214 (HMGB1A-s001) pNIC-CTHF purified protein sequence

<400>  152

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys 
                85                  90                  95      


Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys 
            100                 105                 110         


Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys 
        115                 120                 125             


Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr 
    130                 135                 140                 


Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala 
145                 150                 155                 160 


Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val Val 
                165                 170                 175     


Lys Ala Glu Lys Ser Lys Lys Lys Lys Glu Glu Glu Glu Asp Glu Glu 
            180                 185                 190         


Asp Glu Glu Asp Glu Glu Glu Glu Glu Asp Glu Glu Asp Glu Asp Glu 
        195                 200                 205             


Glu Glu Asp Asp Asp Asp Glu Ala Glu Asn Leu Tyr Phe Gln 
    210                 215                 220         


<210>  153
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  dBB12L pNIC-CTHF

<400>  153

Met Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu 
1               5                   10                  15      


Phe Cys Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu 
            20                  25                  30          


Ser Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr 
        35                  40                  45              


Ala Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys 
    50                  55                  60                  


Glu Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro 
65                  70                  75                  80  


Asp Ala Ala Lys Lys Gly Val Lys Asp Pro Asn Ala Pro Lys Arg Pro 
                85                  90                  95      


Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys Ile Lys 
            100                 105                 110         


Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys Leu Gly 
        115                 120                 125             


Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr Glu Lys 
    130                 135                 140                 


Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala Ala Tyr 
145                 150                 155                 160 


Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val 
                165                 170             


<210>  154
<211>  180
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  dBB12L pNIC-CTHF purified protein sequence

<400>  154

Met Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu 
1               5                   10                  15      


Phe Cys Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu 
            20                  25                  30          


Ser Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr 
        35                  40                  45              


Ala Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys 
    50                  55                  60                  


Glu Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro 
65                  70                  75                  80  


Asp Ala Ala Lys Lys Gly Val Lys Asp Pro Asn Ala Pro Lys Arg Pro 
                85                  90                  95      


Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys Ile Lys 
            100                 105                 110         


Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys Leu Gly 
        115                 120                 125             


Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr Glu Lys 
    130                 135                 140                 


Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala Ala Tyr 
145                 150                 155                 160 


Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val Ala Glu Asn 
                165                 170                 175     


Leu Tyr Phe Gln 
            180 


<210>  155
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3S-HMGB1 1-214 (HMGB1A-s002) pNIC-CTHF

<400>  155

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Ser Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Ser Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys 
                85                  90                  95      


Arg Pro Pro Ser Ala Phe Phe Leu Phe Ser Ser Glu Tyr Arg Pro Lys 
            100                 105                 110         


Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys 
        115                 120                 125             


Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr 
    130                 135                 140                 


Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala 
145                 150                 155                 160 


Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val Val 
                165                 170                 175     


Lys Ala Glu Lys Ser Lys Lys Lys Lys Glu Glu Glu Glu Asp Glu Glu 
            180                 185                 190         


Asp Glu Glu Asp Glu Glu Glu Glu Glu Asp Glu Glu Asp Glu Asp Glu 
        195                 200                 205             


Glu Glu Asp Asp Asp Asp Glu 
    210                 215 


<210>  156
<211>  222
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3S-HMGB1 1-214 (HMGB1A-s002) pNIC-CTHF purified protein sequence

<400>  156

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Ser Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Ser Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys 
                85                  90                  95      


Arg Pro Pro Ser Ala Phe Phe Leu Phe Ser Ser Glu Tyr Arg Pro Lys 
            100                 105                 110         


Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys 
        115                 120                 125             


Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr 
    130                 135                 140                 


Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala 
145                 150                 155                 160 


Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val Val 
                165                 170                 175     


Lys Ala Glu Lys Ser Lys Lys Lys Lys Glu Glu Glu Glu Asp Glu Glu 
            180                 185                 190         


Asp Glu Glu Asp Glu Glu Glu Glu Glu Asp Glu Glu Asp Glu Asp Glu 
        195                 200                 205             


Glu Glu Asp Asp Asp Asp Glu Ala Glu Asn Leu Tyr Phe Gln 
    210                 215                 220         


<210>  157
<211>  185
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-184 pNIC-CTHF

<400>  157

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys 
                85                  90                  95      


Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys 
            100                 105                 110         


Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys 
        115                 120                 125             


Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr 
    130                 135                 140                 


Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala 
145                 150                 155                 160 


Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val Val 
                165                 170                 175     


Lys Ala Glu Lys Ser Lys Lys Lys Lys 
            180                 185 


<210>  158
<211>  192
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-184 pNIC-CTHF purified protein sequence

<400>  158

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys 
                85                  90                  95      


Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys 
            100                 105                 110         


Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys 
        115                 120                 125             


Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr 
    130                 135                 140                 


Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala 
145                 150                 155                 160 


Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val Val 
                165                 170                 175     


Lys Ala Glu Lys Ser Lys Lys Lys Lys Ala Glu Asn Leu Tyr Phe Gln 
            180                 185                 190         


<210>  159
<211>  165
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-164 pNIC-CTHF

<400>  159

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys 
                85                  90                  95      


Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys 
            100                 105                 110         


Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys 
        115                 120                 125             


Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr 
    130                 135                 140                 


Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala 
145                 150                 155                 160 


Ala Tyr Arg Ala Lys 
                165 


<210>  160
<211>  172
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wt HMGB1 1-164 pNIC-CTHF purified protein sequence

<400>  160

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys 
                85                  90                  95      


Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys 
            100                 105                 110         


Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys 
        115                 120                 125             


Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr 
    130                 135                 140                 


Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala 
145                 150                 155                 160 


Ala Tyr Arg Ala Lys Ala Glu Asn Leu Tyr Phe Gln 
                165                 170         


<210>  161
<211>  88
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 1-88, biotinylated pNIC-Bio3

<400>  161

Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr Ala 
1               5                   10                  15      


Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro Asp 
            20                  25                  30          


Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg Trp 
        35                  40                  45              


Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala Lys 
    50                  55                  60                  


Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro Pro 
65                  70                  75                  80  


Lys Gly Glu Thr Lys Lys Lys Phe 
                85              


<210>  162
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 1-88, biotinylated pNIC-Bio3 purified protein sequence

<400>  162

Ser Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser 
1               5                   10                  15      


Tyr Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His 
            20                  25                  30          


Pro Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu 
        35                  40                  45              


Arg Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met 
    50                  55                  60                  


Ala Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile 
65                  70                  75                  80  


Pro Pro Lys Gly Glu Thr Lys Lys Lys Phe Ser Ser Lys Gly Gly Tyr 
                85                  90                  95      


Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
            100                 105                 110     


<210>  163
<211>  86
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 89-174, biotinylated pNIC-Bio3

<400>  163

Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe 
1               5                   10                  15      


Cys Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser 
            20                  25                  30          


Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala 
        35                  40                  45              


Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu 
    50                  55                  60                  


Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp 
65                  70                  75                  80  


Ala Ala Lys Lys Gly Val 
                85      


<210>  164
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 89-174, biotinylated pNIC-Bio3 purified protein sequence

<400>  164

Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe 
1               5                   10                  15      


Cys Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser 
            20                  25                  30          


Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala 
        35                  40                  45              


Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu 
    50                  55                  60                  


Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp 
65                  70                  75                  80  


Ala Ala Lys Lys Gly Val Ser Ser Lys Gly Gly Tyr Gly Leu Asn Asp 
                85                  90                  95      


Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
            100                 105         


<210>  165
<211>  89
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 1-88 pNIC-CTHF

<400>  165

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe 
                85                  


<210>  166
<211>  96
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 1-88 pNIC-CTHF purified protein sequence

<400>  166

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Ala Glu Asn Leu Tyr Phe Gln 
                85                  90                  95      


<210>  167
<211>  87
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 89-174 pNIC-CTHF

<400>  167

Met Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu 
1               5                   10                  15      


Phe Cys Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu 
            20                  25                  30          


Ser Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr 
        35                  40                  45              


Ala Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys 
    50                  55                  60                  


Glu Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro 
65                  70                  75                  80  


Asp Ala Ala Lys Lys Gly Val 
                85          


<210>  168
<211>  94
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 89-174 pNIC-CTHF purified protein sequence

<400>  168

Met Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu 
1               5                   10                  15      


Phe Cys Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu 
            20                  25                  30          


Ser Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr 
        35                  40                  45              


Ala Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys 
    50                  55                  60                  


Glu Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro 
65                  70                  75                  80  


Asp Ala Ala Lys Lys Gly Val Ala Glu Asn Leu Tyr Phe Gln 
                85                  90                  


<210>  169
<211>  71
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 8-78, biotinylated pNIC-Bio3

<400>  169

Pro Arg Gly Lys Met Ser Ser Tyr Ala Phe Phe Val Gln Thr Cys Arg 
1               5                   10                  15      


Glu Glu His Lys Lys Lys His Pro Asp Ala Ser Val Asn Phe Ser Glu 
            20                  25                  30          


Phe Ser Lys Lys Cys Ser Glu Arg Trp Lys Thr Met Ser Ala Lys Glu 
        35                  40                  45              


Lys Gly Lys Phe Glu Asp Met Ala Lys Ala Asp Lys Ala Arg Tyr Glu 
    50                  55                  60                  


Arg Glu Met Lys Thr Tyr Ile 
65                  70      


<210>  170
<211>  94
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 8-78, biotinylated pNIC-Bio3 purified protein sequence

<400>  170

Ser Met Pro Arg Gly Lys Met Ser Ser Tyr Ala Phe Phe Val Gln Thr 
1               5                   10                  15      


Cys Arg Glu Glu His Lys Lys Lys His Pro Asp Ala Ser Val Asn Phe 
            20                  25                  30          


Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg Trp Lys Thr Met Ser Ala 
        35                  40                  45              


Lys Glu Lys Gly Lys Phe Glu Asp Met Ala Lys Ala Asp Lys Ala Arg 
    50                  55                  60                  


Tyr Glu Arg Glu Met Lys Thr Tyr Ile Ser Ser Lys Gly Gly Tyr Gly 
65                  70                  75                  80  


Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
                85                  90                  


<210>  171
<211>  69
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 94-162, biotinylated pNIC-Bio3

<400>  171

Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg 
1               5                   10                  15      


Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala 
            20                  25                  30          


Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln 
        35                  40                  45              


Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp 
    50                  55                  60                  


Ile Ala Ala Tyr Arg 
65                  


<210>  172
<211>  90
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HMGB1 94-162, biotinylated pNIC-Bio3 purified protein sequence

<400>  172

Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg 
1               5                   10                  15      


Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala 
            20                  25                  30          


Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln 
        35                  40                  45              


Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp 
    50                  55                  60                  


Ile Ala Ala Tyr Arg Ser Ser Lys Gly Gly Tyr Gly Leu Asn Asp Ile 
65                  70                  75                  80  


Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
                85                  90  


<210>  173
<211>  165
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wt CXCL12 with SUMO pDsbC-HT-Cbio

<400>  173

Met Ser Asp Gln Glu Ala Lys Pro Ser Thr Glu Asp Leu Gly Asp Lys 
1               5                   10                  15      


Lys Glu Gly Glu Tyr Ile Lys Leu Lys Val Ile Gly Gln Asp Ser Ser 
            20                  25                  30          


Glu Ile His Phe Lys Val Lys Met Thr Thr His Leu Lys Lys Leu Lys 
        35                  40                  45              


Glu Ser Tyr Cys Gln Arg Gln Gly Val Pro Met Asn Ser Leu Arg Phe 
    50                  55                  60                  


Leu Phe Glu Gly Gln Arg Ile Ala Asp Asn His Thr Pro Lys Glu Leu 
65                  70                  75                  80  


Gly Met Glu Glu Glu Asp Val Ile Glu Val Tyr Gln Glu Gln Thr Gly 
                85                  90                  95      


Gly Lys Pro Val Ser Leu Ser Tyr Arg Cys Pro Cys Arg Phe Phe Glu 
            100                 105                 110         


Ser His Val Ala Arg Ala Asn Val Lys His Leu Lys Ile Leu Asn Thr 
        115                 120                 125             


Pro Asn Cys Ala Leu Gln Ile Val Ala Arg Leu Lys Asn Asn Asn Arg 
    130                 135                 140                 


Gln Val Cys Ile Asp Pro Lys Leu Lys Trp Ile Gln Glu Tyr Leu Glu 
145                 150                 155                 160 


Lys Ala Leu Asn Lys 
                165 


<210>  174
<211>  68
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wt CXCL12 with SUMO pDsbC-HT-CBio purified protein sequence

<400>  174

Lys Pro Val Ser Leu Ser Tyr Arg Cys Pro Cys Arg Phe Phe Glu Ser 
1               5                   10                  15      


His Val Ala Arg Ala Asn Val Lys His Leu Lys Ile Leu Asn Thr Pro 
            20                  25                  30          


Asn Cys Ala Leu Gln Ile Val Ala Arg Leu Lys Asn Asn Asn Arg Gln 
        35                  40                  45              


Val Cys Ile Asp Pro Lys Leu Lys Trp Ile Gln Glu Tyr Leu Glu Lys 
    50                  55                  60                  


Ala Leu Asn Lys 
65              


<210>  175
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glycine-Serine Linker

<400>  175

Gly Ser Gly Ser Gly 
1               5   


<210>  176
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glycine-Serine Linker

<400>  176

Gly Ser Ser Ser Gly 
1               5   


<210>  177
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glycine-Serine Linker

<400>  177

Gly Ser Ser Gly Ser 
1               5   


<210>  178
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glycine-Serine Linker

<400>  178

Gly Gly Ser Gly Gly 
1               5   


